AlfalfaGEDB | Msa0751280

M. sativa cultivar zhongmu-4 / Msa0751280

KEGG: -
GO: -
PFAM: PF13302.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751280	sp\|P05332\|YP20_BACLI	30.723	166	103	4	19	177	2	162	1.23e-17	79.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751280	G7K3P7	87.640	178	20	1	1	178	1	176	1.56e-109	320

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TFs/TRs:

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Gene ID	Type	Classification
Msa0751280	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751280	MtrunA17_Chr5g0394631	85.083	181	22	3	1	178	208	386	7.58e-105	306
Msa0751280	MtrunA17_Chr5g0394631	65.341	176	54	3	9	178	1	175	1.89e-70	218
Msa0751280	MtrunA17_Chr5g0394621	71.023	176	44	3	9	178	1	175	1.60e-82	242
Msa0751280	MtrunA17_Chr1g0204951	61.677	167	57	4	16	178	12	175	2.12e-66	201
Msa0751280	MtrunA17_Chr1g0204971	59.627	161	60	3	20	178	18	175	1.01e-64	197
Msa0751280	MtrunA17_Chr7g0223361	45.570	158	81	3	20	176	3	156	6.72e-39	130
Msa0751280	MtrunA17_Chr7g0273661	43.312	157	83	3	21	175	4	156	1.07e-37	127
Msa0751280	MtrunA17_Chr7g0223351	43.671	158	81	4	24	179	7	158	1.51e-35	122

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751280	AT2G32030.1	48.045	179	80	6	1	176	3	171	2.07e-47	153
Msa0751280	AT2G32020.1	50.641	156	67	5	24	176	18	166	2.74e-45	147
Msa0751280	AT3G22560.1	39.130	161	90	4	20	176	2	158	3.77e-30	108

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Find 34 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCCAATAGCATGATCGTTA+TGG	0.457877	5_2:-4794233	None:intergenic
ACCTCGATGATCTCATGGTT+TGG	0.401354	5_2:+4794097	Msa0751280:CDS
AGGAAGTATGTATTCTTTAA+AGG	0.161729	5_2:+4794498	Msa0751280:CDS
AGGATCACATGTGCAGAACT+TGG	0.424567	5_2:+4794294	Msa0751280:CDS
ATGTTCTAGGCTCTAAATAC+TGG	0.313478	5_2:+4794319	Msa0751280:CDS
ATTCAGTGAATTTCCATACT+TGG	0.413337	5_2:+4794389	Msa0751280:CDS
CAAAGAGTAGTTGAGAAAGT+TGG	0.450786	5_2:+4794453	Msa0751280:CDS
CAAATCAATGATCTCTTCTT+TGG	0.204931	5_2:-4794040	None:intergenic
CACATGTGCTGTGAAACAAA+TGG	0.647600	5_2:+4794356	Msa0751280:CDS
CATTTGTTTCACAGCACATG+TGG	0.625218	5_2:-4794355	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAATCAATGATCTCTTCTT+TGG	-	chr5_2:4794043-4794062	None:intergenic	30.0%
TCATTAAAAATATGGCAAGC+AGG	+	chr5_2:4794187-4794206	Msa0751280:CDS	30.0%
ATTCAGTGAATTTCCATACT+TGG	+	chr5_2:4794389-4794408	Msa0751280:CDS	30.0%
GGTCAGAAAGATGAAAAGAT+CGG	-	chr5_2:4794080-4794099	None:intergenic	35.0%
TTGGCATACTGATGAAAAAG+TGG	+	chr5_2:4794116-4794135	Msa0751280:CDS	35.0%
CTTATCATCAAGTGAACTCA+AGG	-	chr5_2:4794271-4794290	None:intergenic	35.0%
TGCTGTGAAACAAATGGTTA+AGG	+	chr5_2:4794362-4794381	Msa0751280:CDS	35.0%
GTGAATTTCCATACTTGGAA+AGG	+	chr5_2:4794394-4794413	Msa0751280:CDS	35.0%
CAAAGAGTAGTTGAGAAAGT+TGG	+	chr5_2:4794453-4794472	Msa0751280:CDS	35.0%
TTCAAAAAGAGGGAGTTCTT+AGG	+	chr5_2:4794478-4794497	Msa0751280:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	4793994	4794536	4793994	ID=Msa0751280;Name=Msa0751280
chr5_2	mRNA	4793994	4794536	4793994	ID=Msa0751280-mRNA-1;Parent=Msa0751280;Name=Msa0751280-mRNA-1;_AED=0.46;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|180
chr5_2	exon	4793994	4794536	4793994	ID=Msa0751280-mRNA-1:exon:1905;Parent=Msa0751280-mRNA-1
chr5_2	CDS	4793994	4794536	4793994	ID=Msa0751280-mRNA-1:cds;Parent=Msa0751280-mRNA-1

Gene Sequence

>Msa0751280

ATGGAGCAAAACACAAGTATTATGATAGAGCGACTTTCTATTTCTTCCAAAGAAGAGATCATTGATTTGAACCAAATCACACTCCGATCTTTTCATCTTTCTGACCTCGATGATCTCATGGTTTGGCATACTGATGAAAAAGTGGCCAAGTTCTGGGGTGAACCTTACACCAGCAAAGACCAAGGCATCAACTTCATTAAAAATATGGCAAGCAGGTATCTAAGTTGCAAAGCAATATGCCATAACGATCATGCTATTGGGTGTATTAAGTTGTCCTTGAGTTCACTTGATGATAAGAGGAGGATCACATGTGCAGAACTTGGGTATGTTCTAGGCTCTAAATACTGGGGTAAAGGGATTGCCACATGTGCTGTGAAACAAATGGTTAAGGTTGCATTCAGTGAATTTCCATACTTGGAAAGGCTTCAAGCTCTTGTTGATGTGGATAATTTAAGGTCGCAAAGAGTAGTTGAGAAAGTTGGTTTTCAAAAAGAGGGAGTTCTTAGGAAGTATGTATTCTTTAAAGGAAAAACAGAGATATGA

Protein sequence

>Msa0751280

MEQNTSIMIERLSISSKEEIIDLNQITLRSFHLSDLDDLMVWHTDEKVAKFWGEPYTSKDQGINFIKNMASRYLSCKAICHNDHAIGCIKLSLSSLDDKRRITCAELGYVLGSKYWGKGIATCAVKQMVKVAFSEFPYLERLQALVDVDNLRSQRVVEKVGFQKEGVLRKYVFFKGKTEI*