AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0752730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0752730 MtrunA17_Chr5g0396901 97.424 893 23 0 1 893 25 917 0.0 1803
Msa0752730 MtrunA17_Chr8g0347481 53.167 600 247 8 1 595 1 571 0.0 632
Msa0752730 MtrunA17_Chr4g0042731 29.773 309 168 15 247 539 142 417 1.03e-24 108
Msa0752730 MtrunA17_Chr4g0075541 31.474 251 130 9 280 525 131 344 1.44e-24 107
Msa0752730 MtrunA17_Chr4g0059121 29.845 258 142 10 282 536 104 325 1.17e-23 103
Msa0752730 MtrunA17_Chr4g0065211 30.556 288 150 11 264 535 1139 1392 2.16e-23 107
Msa0752730 MtrunA17_Chr3g0117731 30.233 258 141 9 282 536 103 324 2.93e-23 102
Msa0752730 MtrunA17_Chr5g0406251 26.104 249 146 8 282 528 337 549 4.95e-23 104
Msa0752730 MtrunA17_Chr4g0047191 27.273 253 145 10 282 531 92 308 3.81e-22 99.4
Msa0752730 MtrunA17_Chr4g0047211 27.273 253 145 10 282 531 87 303 4.30e-22 99.0
Msa0752730 MtrunA17_Chr4g0047201 26.616 263 155 9 282 541 248 475 6.00e-22 100
Msa0752730 MtrunA17_Chr2g0305141 29.373 303 166 12 264 549 1101 1372 8.03e-22 102
Msa0752730 MtrunA17_Chr5g0397931 28.247 308 163 17 246 534 421 689 8.55e-22 101
Msa0752730 MtrunA17_Chr1g0211611 26.908 249 144 8 282 528 343 555 9.28e-22 100
Msa0752730 MtrunA17_Chr2g0282321 25.954 262 158 9 282 541 209 436 7.01e-21 97.1
Msa0752730 MtrunA17_Chr6g0461841 31.618 272 152 8 270 536 1001 1243 9.87e-21 98.6
Msa0752730 MtrunA17_Chr6g0450871 28.137 263 143 10 279 526 118 349 1.28e-20 95.1
Msa0752730 MtrunA17_Chr7g0244621 29.134 254 143 9 278 523 82 306 1.71e-20 94.4
Msa0752730 MtrunA17_Chr3g0143531 27.092 251 145 9 282 530 67 281 1.78e-20 92.8
Msa0752730 MtrunA17_Chr4g0059181 26.996 263 146 8 279 526 135 366 1.85e-20 94.7
Msa0752730 MtrunA17_Chr3g0117811 25.779 353 192 13 212 526 17 337 1.96e-20 94.4
Msa0752730 MtrunA17_Chr2g0310761 27.687 307 166 12 242 526 933 1205 2.33e-20 97.4
Msa0752730 MtrunA17_Chr8g0357611 26.687 326 185 11 222 526 1344 1636 3.15e-20 97.1
Msa0752730 MtrunA17_Chr1g0202101 24.315 292 167 10 245 519 273 527 5.09e-20 95.1
Msa0752730 MtrunA17_Chr4g0072741 29.153 295 163 10 247 524 834 1099 6.65e-20 95.9
Msa0752730 MtrunA17_Chr2g0310781 27.213 305 166 13 242 524 839 1109 4.52e-18 89.7
Msa0752730 MtrunA17_Chr4g0068411 28.772 285 152 13 246 516 458 705 5.49e-18 89.0
Msa0752730 MtrunA17_Chr6g0449241 26.316 304 172 12 242 526 20 290 1.50e-17 85.5
Msa0752730 MtrunA17_Chr2g0294351 27.857 280 145 14 265 528 485 723 9.78e-17 85.1
Msa0752730 MtrunA17_Chr3g0090261 25.101 247 146 9 280 523 126 336 1.32e-16 82.8
Msa0752730 MtrunA17_Chr3g0116981 25.413 303 169 11 242 523 20 286 1.33e-16 82.4
Msa0752730 MtrunA17_Chr1g0196631 28.629 248 139 7 289 530 955 1170 1.65e-16 84.7
Msa0752730 MtrunA17_Chr5g0403721 26.531 245 150 9 279 523 28 242 4.35e-16 80.9
Msa0752730 MtrunA17_Chr1g0171571 28.696 230 115 9 326 526 106 315 6.18e-16 82.4
Msa0752730 MtrunA17_Chr5g0443471 27.881 269 156 11 280 541 205 442 1.02e-15 80.9
Msa0752730 MtrunA17_Chr5g0394681 27.461 193 111 6 334 526 201 364 5.03e-15 78.2
Msa0752730 MtrunA17_Chr1g0191171 24.252 301 173 13 246 530 687 948 5.31e-15 79.7
Msa0752730 MtrunA17_Chr5g0427651 25.581 301 165 15 247 528 645 905 1.18e-14 78.6
Msa0752730 MtrunA17_Chr1g0157231 25.789 190 105 6 282 470 188 342 1.92e-14 76.3
Msa0752730 MtrunA17_Chr8g0380551 28.226 248 136 11 282 519 266 481 3.55e-14 76.3
Msa0752730 MtrunA17_Chr1g0179331 24.497 298 172 12 247 528 554 814 9.28e-14 75.5
Msa0752730 MtrunA17_Chr4g0000431 23.288 292 182 10 243 521 19 281 2.43e-13 73.9
Msa0752730 MtrunA17_Chr3g0105891 25.410 244 143 8 285 523 371 580 5.61e-13 72.8
Msa0752730 MtrunA17_Chr1g0209511 26.766 269 150 11 277 524 352 594 6.14e-13 72.8
Msa0752730 MtrunA17_Chr3g0115171 26.778 239 137 8 277 513 322 524 1.68e-12 71.2
Msa0752730 MtrunA17_Chr1g0171451 28.000 275 149 13 265 526 433 671 2.05e-12 71.2
Msa0752730 MtrunA17_Chr4g0051191 27.273 242 134 8 277 514 203 406 3.97e-12 69.3
Msa0752730 MtrunA17_Chr8g0359691 26.733 303 147 16 255 523 674 935 5.63e-12 70.1
Msa0752730 MtrunA17_Chr6g0450571 23.675 283 178 9 243 514 11 266 6.63e-12 69.3
Msa0752730 MtrunA17_Chr4g0053411 25.298 336 168 15 239 523 458 761 7.38e-12 69.3
Msa0752730 MtrunA17_Chr2g0280191 26.190 252 131 11 241 469 676 895 1.22e-11 68.9
Msa0752730 MtrunA17_Chr1g0209521 24.752 303 166 13 255 527 368 638 2.01e-11 67.8
Msa0752730 MtrunA17_Chr8g0384301 26.293 232 133 8 285 514 349 544 3.35e-11 67.0
Msa0752730 MtrunA17_Chr5g0423121 25.769 260 144 11 277 521 377 602 4.76e-11 66.6
Msa0752730 MtrunA17_Chr4g0041201 26.316 285 160 13 257 531 559 803 5.37e-11 66.6
Msa0752730 MtrunA17_Chr7g0276571 26.374 273 142 13 280 528 457 694 5.62e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0752730 AT1G64300.2 52.510 777 297 14 1 767 1 715 0.0 786
Msa0752730 AT1G64300.1 52.510 777 297 14 1 767 1 715 0.0 786
Msa0752730 AT5G41730.2 52.291 742 295 11 1 726 1 699 0.0 762
Msa0752730 AT5G41730.1 52.291 742 295 11 1 726 1 699 0.0 762
Msa0752730 AT1G01450.1 53.333 390 152 7 211 594 2 367 8.50e-131 401
Msa0752730 AT1G14000.1 31.494 308 164 17 247 539 161 436 1.74e-24 108
Msa0752730 AT1G62400.1 30.645 248 131 8 280 523 130 340 5.73e-24 105
Msa0752730 AT3G46930.2 25.959 339 198 10 224 545 179 481 9.25e-24 106
Msa0752730 AT3G46930.1 25.959 339 198 10 224 545 144 446 1.42e-23 105
Msa0752730 AT3G46920.1 28.378 370 191 16 192 530 750 1076 1.30e-22 105
Msa0752730 AT3G46920.2 28.378 370 191 16 192 530 816 1142 1.32e-22 105
Msa0752730 AT5G58950.1 27.451 255 140 11 276 523 247 463 1.65e-21 99.8
Msa0752730 AT2G35050.1 28.618 304 166 14 242 526 968 1239 4.06e-21 100
Msa0752730 AT2G35050.2 28.618 304 166 14 242 526 968 1239 4.06e-21 100
Msa0752730 AT5G01850.1 27.642 246 145 7 282 523 63 279 1.05e-20 94.7
Msa0752730 AT4G38470.2 25.794 252 149 8 282 531 332 547 3.15e-20 96.3
Msa0752730 AT5G57610.1 27.723 303 173 10 247 532 780 1053 3.42e-20 97.1
Msa0752730 AT5G01850.2 29.907 214 120 6 314 523 116 303 1.27e-19 92.0
Msa0752730 AT4G35780.1 24.590 244 146 8 282 523 334 541 1.42e-19 94.0
Msa0752730 AT4G38470.1 25.506 247 146 8 282 526 332 542 1.62e-19 94.0
Msa0752730 AT5G50000.1 26.616 263 147 8 279 526 128 359 1.78e-19 92.0
Msa0752730 AT3G01490.1 28.244 262 144 11 279 526 154 385 2.39e-19 92.0
Msa0752730 AT1G04700.1 30.380 237 137 6 289 524 821 1030 2.41e-19 94.4
Msa0752730 AT1G04700.2 30.380 237 137 6 289 524 821 1030 2.41e-19 94.4
Msa0752730 AT2G17700.1 26.000 250 145 10 282 528 328 540 3.79e-19 92.8
Msa0752730 AT3G24715.1 28.873 284 172 9 243 522 842 1099 4.01e-19 93.6
Msa0752730 AT3G24715.3 28.873 284 172 9 243 522 842 1099 4.01e-19 93.6
Msa0752730 AT3G24715.2 28.873 284 172 9 243 522 842 1099 4.01e-19 93.6
Msa0752730 AT1G79570.2 26.403 303 175 10 242 526 958 1230 4.06e-19 93.6
Msa0752730 AT1G79570.1 26.403 303 175 10 242 526 958 1230 4.06e-19 93.6
Msa0752730 AT1G16270.1 27.143 280 167 8 258 533 890 1136 4.46e-19 93.6
Msa0752730 AT1G16270.2 27.143 280 167 8 258 533 890 1136 4.46e-19 93.6
Msa0752730 AT1G16270.3 27.143 280 167 8 258 533 890 1136 4.46e-19 93.6
Msa0752730 AT4G31170.7 27.092 251 136 9 280 523 175 385 4.72e-19 91.3
Msa0752730 AT4G31170.6 27.092 251 136 9 280 523 175 385 4.72e-19 91.3
Msa0752730 AT4G31170.5 27.092 251 136 9 280 523 175 385 4.72e-19 91.3
Msa0752730 AT4G31170.1 27.092 251 136 9 280 523 175 385 4.72e-19 91.3
Msa0752730 AT4G31170.2 27.092 251 136 9 280 523 175 385 4.72e-19 91.3
Msa0752730 AT4G31170.3 27.092 251 136 9 280 523 175 385 4.72e-19 91.3
Msa0752730 AT4G31170.8 27.092 251 136 9 280 523 175 385 4.72e-19 91.3
Msa0752730 AT4G23050.2 27.815 302 158 14 246 528 466 726 2.80e-18 90.5
Msa0752730 AT4G23050.1 27.815 302 158 14 246 528 465 725 2.82e-18 90.5
Msa0752730 AT3G06630.2 28.283 297 160 15 247 528 433 691 3.25e-18 90.1
Msa0752730 AT5G66710.1 25.641 273 163 8 278 544 113 351 4.49e-18 88.2
Msa0752730 AT2G40860.4 30.964 197 98 6 340 514 24 204 1.15e-17 88.2
Msa0752730 AT5G49470.3 27.857 280 145 14 265 528 510 748 1.21e-17 88.6
Msa0752730 AT3G50730.1 25.000 248 157 8 277 523 77 296 1.23e-17 86.3
Msa0752730 AT5G49470.4 27.857 280 145 14 265 528 484 722 1.41e-17 88.2
Msa0752730 AT2G40860.2 30.144 209 108 6 340 526 122 314 1.53e-17 87.8
Msa0752730 AT5G11850.1 25.676 296 169 12 246 527 607 865 1.58e-17 88.2
Msa0752730 AT5G49470.7 27.857 280 145 14 265 528 383 621 1.77e-17 87.8
Msa0752730 AT2G40860.1 30.964 197 98 6 340 514 122 302 1.82e-17 87.8
Msa0752730 AT2G40860.3 30.964 197 98 6 340 514 122 302 1.86e-17 87.4
Msa0752730 AT3G27560.1 27.600 250 146 10 282 526 71 290 1.88e-17 85.5
Msa0752730 AT5G49470.1 27.857 280 145 14 265 528 223 461 2.36e-17 86.7
Msa0752730 AT2G24360.1 26.050 238 143 7 286 523 180 384 2.56e-17 85.9
Msa0752730 AT5G40540.1 27.668 253 148 11 276 523 64 286 3.12e-17 84.7
Msa0752730 AT3G22750.1 24.528 265 151 8 279 526 120 352 3.78e-17 85.1
Msa0752730 AT3G57830.3 27.737 274 162 10 279 527 398 660 6.71e-17 85.9
Msa0752730 AT3G57830.1 27.737 274 162 10 279 527 398 660 6.71e-17 85.9
Msa0752730 AT4G18950.2 28.571 252 138 10 282 526 200 416 9.19e-17 84.3
Msa0752730 AT4G18950.1 28.571 252 138 10 282 526 200 416 1.01e-16 84.3
Msa0752730 AT3G63260.1 24.060 266 155 8 279 526 129 365 1.35e-16 83.6
Msa0752730 AT4G24480.1 26.073 303 166 13 247 528 668 933 1.94e-16 84.7
Msa0752730 AT5G50180.1 27.236 246 145 9 282 523 65 280 2.26e-16 82.0
Msa0752730 AT1G67890.1 27.957 279 145 15 265 528 506 743 2.30e-16 84.3
Msa0752730 AT2G42290.1 28.731 268 159 9 282 527 389 646 2.45e-16 84.0
Msa0752730 AT5G40540.3 29.439 214 120 9 314 523 3 189 4.97e-16 79.3
Msa0752730 AT4G24480.2 25.589 297 170 13 247 528 668 928 5.49e-16 83.6
Msa0752730 AT4G24480.3 25.589 297 170 13 247 528 668 928 5.81e-16 83.2
Msa0752730 AT5G40540.2 29.439 214 120 9 314 523 24 210 8.38e-16 79.3
Msa0752730 AT3G06620.1 27.798 277 149 13 265 528 513 751 9.64e-16 82.4
Msa0752730 AT3G58760.1 29.000 300 156 18 247 526 165 427 7.23e-15 78.6
Msa0752730 AT3G50720.1 26.459 257 160 9 276 531 88 316 8.28e-15 77.8
Msa0752730 AT3G58760.3 29.000 300 156 18 247 526 228 490 8.90e-15 78.6
Msa0752730 AT3G58760.5 29.000 300 156 18 247 526 228 490 8.90e-15 78.6
Msa0752730 AT3G06630.1 28.261 276 145 15 247 507 433 670 1.01e-14 79.0
Msa0752730 AT4G14780.1 26.316 190 109 5 339 526 178 338 1.08e-14 77.4
Msa0752730 AT3G46930.4 24.715 263 149 8 224 473 179 405 3.72e-14 76.3
Msa0752730 AT3G46930.3 24.715 263 149 8 224 473 179 405 4.03e-14 76.3
Msa0752730 AT2G31800.1 25.987 304 177 14 243 530 188 459 4.44e-14 76.3
Msa0752730 AT3G46930.5 24.715 263 149 8 224 473 144 370 6.70e-14 75.1
Msa0752730 AT3G46930.6 24.715 263 149 8 224 473 83 309 8.31e-14 74.3
Msa0752730 AT3G58640.1 28.205 273 146 13 257 519 565 797 9.76e-14 75.9
Msa0752730 AT3G58640.2 28.205 273 146 13 257 519 565 797 9.76e-14 75.9
Msa0752730 AT1G50240.3 24.211 285 161 12 243 513 1 244 2.42e-13 75.1
Msa0752730 AT1G50240.2 24.211 285 161 12 243 513 1 244 2.42e-13 75.1
Msa0752730 AT5G66850.1 25.703 249 153 10 283 530 394 611 3.69e-13 73.9
Msa0752730 AT5G01890.1 23.743 358 209 13 191 527 646 960 3.84e-13 74.3
Msa0752730 AT3G59830.1 27.586 290 162 13 247 519 193 451 3.85e-13 73.2
Msa0752730 AT5G66850.2 25.703 249 153 10 283 530 394 611 4.65e-13 73.6
Msa0752730 AT3G06630.6 28.571 238 120 13 247 470 349 550 6.00e-13 73.2
Msa0752730 AT3G06630.4 28.571 238 120 13 247 470 433 634 6.45e-13 73.2
Msa0752730 AT3G06630.3 28.571 238 120 13 247 470 433 634 6.45e-13 73.2
Msa0752730 AT3G06630.5 28.571 238 120 13 247 470 433 634 6.45e-13 73.2
Msa0752730 AT5G18700.1 25.847 236 142 10 285 520 46 248 8.52e-13 73.2
Msa0752730 AT1G67890.3 27.907 258 130 15 265 507 506 722 3.22e-12 70.9
Msa0752730 AT1G67890.2 27.907 258 130 15 265 507 506 722 3.22e-12 70.9
Msa0752730 AT1G67890.4 27.907 258 130 15 265 507 378 594 4.24e-12 70.5
Msa0752730 AT2G31010.1 25.685 292 159 13 257 538 531 774 5.99e-12 70.1
Msa0752730 AT2G31010.2 25.685 292 159 13 257 538 531 774 5.99e-12 70.1
Msa0752730 AT3G53930.1 27.586 261 134 13 247 491 19 240 7.40e-12 69.7
Msa0752730 AT3G53930.2 27.586 261 134 13 247 491 19 240 7.47e-12 69.7
Msa0752730 AT2G42640.1 28.044 271 141 13 265 524 547 774 8.61e-12 69.7
Msa0752730 AT2G42640.4 28.044 271 141 13 265 524 509 736 1.05e-11 69.3
Msa0752730 AT5G49470.5 28.182 220 104 12 265 469 510 690 1.06e-11 69.3
Msa0752730 AT5G49470.6 28.182 220 104 12 265 469 510 690 1.16e-11 69.3
Msa0752730 AT5G38210.3 24.739 287 162 10 280 544 391 645 1.58e-11 68.6
Msa0752730 AT5G38210.2 25.175 286 162 10 280 544 409 663 1.69e-11 68.6
Msa0752730 AT3G01490.2 27.632 228 122 10 279 492 154 352 3.34e-11 66.6
Msa0752730 AT4G08480.1 26.172 256 147 9 261 514 529 744 3.51e-11 67.8
Msa0752730 AT5G40540.4 28.061 196 114 8 276 469 64 234 5.23e-11 65.1
Msa0752730 AT5G38210.1 25.175 286 162 10 280 544 397 651 5.91e-11 67.0
Msa0752730 AT1G69270.1 25.820 244 144 10 270 510 291 500 6.15e-11 66.6
Msa0752730 AT4G08500.1 26.336 262 153 9 255 514 353 576 6.32e-11 66.6
Msa0752730 AT3G19700.1 28.497 193 105 8 282 469 730 894 6.45e-11 67.0
Msa0752730 AT3G02880.1 31.500 200 99 9 272 467 375 540 6.82e-11 66.6

Find 164 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCCTGCTTTGATATTT+TGG 0.080066 5_2:+6479570 None:intergenic
CTCTCAGATTCTGAGACTTT+TGG 0.146039 5_2:+6478732 None:intergenic
ATCAAACTCACAAGATATTT+TGG 0.164131 5_2:+6479524 None:intergenic
TTGATTCCCTTTGTTAATAT+TGG 0.196770 5_2:+6478704 None:intergenic
CAAGCAAAAGTTGTTGGATT+TGG 0.204645 5_2:-6479851 Msa0752730:CDS
GAGATTTGGCAAACAATATT+TGG 0.205668 5_2:-6480524 Msa0752730:CDS
GATCCTAACAAAACACTAAT+TGG 0.207436 5_2:+6480295 None:intergenic
AATTGCTTGCAAATTTCTTT+TGG 0.212048 5_2:+6480499 None:intergenic
TCTCCTCACTTGGTAATCTT+TGG 0.230174 5_2:+6480273 None:intergenic
GCTTATAAAGTTGTTGAAAA+AGG 0.240086 5_2:-6479251 Msa0752730:CDS
GTGTCATCAGAAATTGATTT+TGG 0.241755 5_2:+6479056 None:intergenic
TATTCCTACTATAGATAATA+AGG 0.252638 5_2:-6479195 Msa0752730:CDS
AAGAGATTAACCCTATAATT+TGG 0.257480 5_2:-6479757 Msa0752730:CDS
GTCCTCTTAGAGAGCTTTCC+AGG 0.263161 5_2:-6480807 Msa0752730:CDS
AACTGACCTTGGTCTTTCTT+TGG 0.265277 5_2:+6478966 None:intergenic
GGTTACCAGATCTTTAATTA+TGG 0.266121 5_2:+6479082 None:intergenic
CATAGATATTGCAATATGTT+TGG 0.282076 5_2:+6480124 None:intergenic
TCAGGTGCATACCAAATTAT+AGG 0.292831 5_2:+6479746 None:intergenic
CTGGTTTCTCATCTATTTGT+AGG 0.294229 5_2:-6479466 Msa0752730:CDS
TTTGTCTTTAACCTGTTAAA+TGG 0.297516 5_2:+6478579 None:intergenic
CGGCACATTGGTGAGGTTCT+TGG 0.303933 5_2:-6480994 Msa0752730:CDS
TATGGTCATGTCTCAGATTA+AGG 0.304997 5_2:-6478252 None:intergenic
TGGAAGTCTAAAAGCGCTTA+TGG 0.306735 5_2:-6480974 Msa0752730:CDS
TGAAACATGGCAGCATCTTC+TGG 0.314429 5_2:+6478422 None:intergenic
AAAAGAAATTTGCAAGCAAT+TGG 0.319977 5_2:-6480497 Msa0752730:CDS
GGATTGTCATCTGTTGAAAA+TGG 0.333696 5_2:-6479830 Msa0752730:CDS
ATTCTTTGAAGAAGTGAGTT+TGG 0.334226 5_2:+6480411 None:intergenic
GTCCTTTGATTGCTCACATA+TGG 0.339488 5_2:+6478336 None:intergenic
AGCATTGTGAGGATGCAAAA+AGG 0.341134 5_2:-6478486 Msa0752730:CDS
CATTATCTGGTCGGGTTTCA+AGG 0.346406 5_2:-6478818 Msa0752730:CDS
ATTATACATTAAGCATTGTT+TGG 0.350263 5_2:-6480767 Msa0752730:CDS
TTGACACGTCTTATTCTTGT+TGG 0.355333 5_2:+6478522 None:intergenic
GAAATTGATGAATGGTAATT+CGG 0.363355 5_2:-6480359 Msa0752730:CDS
TCGGGTTTCAAGGACAAATA+AGG 0.364030 5_2:-6478808 Msa0752730:CDS
TTTAAGGAAATTCAATGGTT+AGG 0.370820 5_2:-6480229 Msa0752730:CDS
GAGACCAATCACCATTTAAC+AGG 0.371640 5_2:-6478590 Msa0752730:CDS
ATTGTTTGGATTCAAAAGAT+TGG 0.378122 5_2:-6480753 Msa0752730:CDS
GTGAGAAAGTGATAACAAAT+TGG 0.378799 5_2:+6480147 None:intergenic
TTCTTTGGCTTCATTTGTGA+TGG 0.379395 5_2:+6478981 None:intergenic
TTAACCTGTTAAATGGTGAT+TGG 0.382051 5_2:+6478586 None:intergenic
ATAGATATTGCAATATGTTT+GGG 0.387928 5_2:+6480125 None:intergenic
AGTTCAAGCTGATTTAGTTC+AGG 0.389760 5_2:+6479386 None:intergenic
ATCTCATGCTTCAAATGGCC+AGG 0.399868 5_2:-6479970 Msa0752730:CDS
TTGAAGACAGAACTACAAAA+TGG 0.399911 5_2:-6480843 Msa0752730:CDS
ATTGTAACATTCTTCTTTGA+CGG 0.401100 5_2:+6479011 None:intergenic
TGAGGAGAAGATTGGGAAGG+GGG 0.403016 5_2:-6480258 Msa0752730:CDS
GGGTTAATCTCTTCACCAAT+TGG 0.403263 5_2:+6479767 None:intergenic
CCATATGTTGTTCTTACATA+AGG 0.409101 5_2:+6478270 None:intergenic
TACCAAGTGAGGAGAAGATT+GGG 0.409714 5_2:-6480265 Msa0752730:CDS
ACAGTAGCCACATCCTTTGT+TGG 0.413590 5_2:+6479158 None:intergenic
TTGGATTCAAAAGATTGGTC+AGG 0.420334 5_2:-6480748 Msa0752730:CDS
TCATTACCACCAACATTATC+TGG 0.424357 5_2:-6478831 Msa0752730:CDS
ATGCTGCCATGTTTCAGATA+TGG 0.424537 5_2:-6478415 Msa0752730:CDS
GAGAATGCTGGGGAGATGTC+AGG 0.427765 5_2:-6480634 Msa0752730:CDS
TGCATCCATAATTAAAGATC+TGG 0.429270 5_2:-6479087 Msa0752730:CDS
TTATAAGCAAACATTTCATA+AGG 0.434328 5_2:+6479266 None:intergenic
TTGTAACATTCTTCTTTGAC+GGG 0.441892 5_2:+6479012 None:intergenic
CTCAAAGGGCTCAATGCTAA+TGG 0.444928 5_2:+6478294 None:intergenic
GTAGGATATTACAAGGACTA+AGG 0.446067 5_2:+6479906 None:intergenic
CTCAAAGGACTCAATGGAAA+TGG 0.446507 5_2:+6478360 None:intergenic
TTCCCAATCTTCTCCTCACT+TGG 0.447928 5_2:+6480263 None:intergenic
ATTCCTACTATAGATAATAA+GGG 0.450157 5_2:-6479194 Msa0752730:CDS
TGAAAATGATTATCCTCAAA+AGG 0.452553 5_2:+6479608 None:intergenic
CATCTTCACAAACTGTGCTT+TGG 0.453158 5_2:+6479114 None:intergenic
GCAAGCAATTGGAGAATGCT+TGG 0.459665 5_2:-6480486 Msa0752730:CDS
GAGAACAAAATCTGTCTCCT+TGG 0.464084 5_2:+6480010 None:intergenic
GGAGATGTCAGGGTTGGATC+AGG 0.464317 5_2:-6480623 Msa0752730:CDS
TTACCAAGTGAGGAGAAGAT+TGG 0.469923 5_2:-6480266 Msa0752730:CDS
AGGAATTTAAGGAAATTCAA+TGG 0.476317 5_2:-6480234 Msa0752730:CDS
ACCACCAACATTATCTGGTC+GGG 0.478543 5_2:-6478826 Msa0752730:CDS
CACCGCCAATGAGTCCTTCA+AGG 0.481111 5_2:-6478452 Msa0752730:CDS
AAAGCCGATGCATATAGCTT+TGG 0.483467 5_2:-6479665 Msa0752730:CDS
CAGGTGCATACCAAATTATA+GGG 0.486379 5_2:+6479747 None:intergenic
TCACCAACACATAATCCAAT+TGG 0.489553 5_2:-6479782 Msa0752730:CDS
TTCACCTTCTTTGAATACCC+TGG 0.491717 5_2:+6480789 None:intergenic
TATGGACTCAAAGGACTCAA+TGG 0.498751 5_2:+6478354 None:intergenic
CTACCCTTATTATCTATAGT+AGG 0.503856 5_2:+6479191 None:intergenic
ACCAACCAAAATATCAAAGC+AGG 0.507591 5_2:-6479575 Msa0752730:CDS
GTTCTTACATAAGGACTCAA+AGG 0.508350 5_2:+6478279 None:intergenic
ATTGAAGCGATCGAGAATGC+TGG 0.508806 5_2:-6480646 Msa0752730:CDS
CTTTCCAGGGTATTCAAAGA+AGG 0.511780 5_2:-6480793 Msa0752730:CDS
TCTAGCTCAGTTAAAACCTC+AGG 0.514317 5_2:+6479728 None:intergenic
TTGAAGCGATCGAGAATGCT+GGG 0.515187 5_2:-6480645 Msa0752730:CDS
ACTGATCCGTCTCAGCGTCC+TGG 0.518950 5_2:-6479485 Msa0752730:CDS
CTTCTGGTGTATGACCTTGA+AGG 0.519264 5_2:+6478438 None:intergenic
AGAATGCTTGGAGAGAAGAT+AGG 0.519476 5_2:-6480474 Msa0752730:CDS
AGTTGATGAACAAGGATTTG+TGG 0.524686 5_2:-6480057 Msa0752730:CDS
TGGATTCAAAAGATTGGTCA+GGG 0.524926 5_2:-6480747 Msa0752730:CDS
TGCTGGGGAGATGTCAGGGT+TGG 0.526191 5_2:-6480629 Msa0752730:CDS
GGAAATCCAATATTAACAAA+GGG 0.528711 5_2:-6478710 Msa0752730:CDS
CCTTATGTAAGAACAACATA+TGG 0.529072 5_2:-6478270 Msa0752730:CDS
CTATAACCCCAGTTAAAAGA+AGG 0.532741 5_2:-6478758 Msa0752730:CDS
AGGAAATCCAATATTAACAA+AGG 0.532942 5_2:-6478711 Msa0752730:CDS
TACAAGGACTAAGGTTTCCA+TGG 0.534289 5_2:+6479915 None:intergenic
TATGACCTTGAAGGACTCAT+TGG 0.538589 5_2:+6478447 None:intergenic
CAAGAAAATATGATGTGGAA+TGG 0.539842 5_2:-6480570 Msa0752730:CDS
ACACAATCGCAGATGAGATA+AGG 0.544064 5_2:-6480879 Msa0752730:CDS
GTGCCGGAATGGAACAGCTC+CGG 0.546196 5_2:-6481014 None:intergenic
TTTGTTCTCTATACCTGTGG+TGG 0.547282 5_2:-6479996 Msa0752730:CDS
ATATTATATGTCCTCTCCAT+AGG 0.548736 5_2:+6479308 None:intergenic
ATGCTTGGAGAGAAGATAGG+TGG 0.548742 5_2:-6480471 Msa0752730:CDS
TATAATTTGGTATGCACCTG+AGG 0.550991 5_2:-6479744 Msa0752730:CDS
TTCTTACATAAGGACTCAAA+GGG 0.551714 5_2:+6478280 None:intergenic
GGTTGATCTCATGCTTCAAA+TGG 0.555778 5_2:-6479975 Msa0752730:CDS
AACATATTGCAATATCTATG+TGG 0.560041 5_2:-6480121 Msa0752730:CDS
ACCCTGGAAAGCTCTCTAAG+AGG 0.560256 5_2:+6480805 None:intergenic
ATGGAACAGCTCCGGCACAT+TGG 0.561500 5_2:-6481006 Msa0752730:CDS
AGCAATCAAAGGACTAGAAG+AGG 0.562315 5_2:-6478327 Msa0752730:CDS
GGCGAGCGATATGGAAACAA+AGG 0.564210 5_2:+6479545 None:intergenic
CAAGAACCTCACCAATGTGC+CGG 0.564531 5_2:+6480995 None:intergenic
GTGGAGATATTCAATGCCCC+TGG 0.567677 5_2:+6479952 None:intergenic
GACCTTGAAGGACTCATTGG+CGG 0.570717 5_2:+6478450 None:intergenic
TTGTTGAAGAAATTGATGAA+TGG 0.570718 5_2:-6480367 Msa0752730:CDS
TCACCAATTGGATTATGTGT+TGG 0.571772 5_2:+6479779 None:intergenic
GAGAAACCAGGACGCTGAGA+CGG 0.572704 5_2:+6479479 None:intergenic
TACCACCAACATTATCTGGT+CGG 0.572730 5_2:-6478827 Msa0752730:CDS
GTCTGAGCCAACAAAGGATG+TGG 0.574953 5_2:-6479165 Msa0752730:CDS
TTGCTCACATATGGACTCAA+AGG 0.582014 5_2:+6478345 None:intergenic
GTGGATTGTTGTGACATTGA+AGG 0.582514 5_2:-6479350 Msa0752730:CDS
TCTCATGCTTCAAATGGCCA+GGG 0.583448 5_2:-6479969 Msa0752730:CDS
AGTGAGGAGAAGATTGGGAA+GGG 0.587219 5_2:-6480260 Msa0752730:CDS
TCGATCGCTTCAATAACCGC+AGG 0.587690 5_2:+6480655 None:intergenic
GTGAGGAGAAGATTGGGAAG+GGG 0.591731 5_2:-6480259 Msa0752730:CDS
GAGGCTCATAGAAGCACTCA+AGG 0.595114 5_2:-6480449 Msa0752730:CDS
GTCCATATGTGAGCAATCAA+AGG 0.596259 5_2:-6478338 Msa0752730:CDS
CATACCAAAGCTATATGCAT+CGG 0.599646 5_2:+6479661 None:intergenic
AAAGCTAGAAACTCACAAGA+AGG 0.600879 5_2:-6479881 Msa0752730:CDS
AACTTGATGTCAATGATGAC+TGG 0.601724 5_2:+6478781 None:intergenic
AGAATGCTGGGGAGATGTCA+GGG 0.601811 5_2:-6480633 Msa0752730:CDS
TGAGAGAGAATTGTGGTCCA+AGG 0.604253 5_2:-6480027 Msa0752730:CDS
TCCTCTTAGAGAGCTTTCCA+GGG 0.606681 5_2:-6480806 Msa0752730:CDS
TGAACTTCAGAACCCACCTG+TGG 0.609326 5_2:-6479369 Msa0752730:CDS
TTTCTCTGAGTACTTACAAG+AGG 0.613541 5_2:+6479685 None:intergenic
ACCCGACCAGATAATGTTGG+TGG 0.618092 5_2:+6478825 None:intergenic
AGCATGAGATCAACCACCAC+AGG 0.618436 5_2:+6479983 None:intergenic
CTTGGAGAGAAGATAGGTGG+AGG 0.618932 5_2:-6480468 Msa0752730:CDS
AAAATATCAAAGCAGGAGAG+AGG 0.620693 5_2:-6479568 Msa0752730:CDS
GAAACCCGACCAGATAATGT+TGG 0.622199 5_2:+6478822 None:intergenic
CTACAAATAGATGAGAAACC+AGG 0.623694 5_2:+6479467 None:intergenic
AACTATCCATATCTGAAACA+TGG 0.625924 5_2:+6478409 None:intergenic
ACAGTAATGATAAGGTATTG+TGG 0.632731 5_2:-6480318 Msa0752730:CDS
ATGTCACAACAATCCACAGG+TGG 0.636860 5_2:+6479356 None:intergenic
TCAATGTCACAACAATCCAC+AGG 0.638708 5_2:+6479353 None:intergenic
ATGCTAGAGTGAATATGTCG+TGG 0.640538 5_2:+6480905 None:intergenic
AGGATTGATCAAAACATACT+CGG 0.640765 5_2:+6479406 None:intergenic
CAAAAGTCTCAGAATCTGAG+AGG 0.650694 5_2:-6478731 Msa0752730:CDS
TCTCTAAGAGGACATTCAAG+AGG 0.651164 5_2:+6480817 None:intergenic
ACAGCTCCGGCACATTGGTG+AGG 0.651948 5_2:-6481001 Msa0752730:CDS
GCTTGCAAGAAAATATGATG+TGG 0.653459 5_2:-6480575 Msa0752730:CDS
TAGTGAGTCTGAGCCAACAA+AGG 0.653550 5_2:-6479171 Msa0752730:CDS
CGATCGCTTCAATAACCGCA+GGG 0.654532 5_2:+6480656 None:intergenic
AAGTGAGGAGAAGATTGGGA+AGG 0.655591 5_2:-6480261 Msa0752730:CDS
AATTGGATTATGTGTTGGTG+AGG 0.655784 5_2:+6479784 None:intergenic
TGTGACGGAGTTGATGAACA+AGG 0.656381 5_2:-6480065 Msa0752730:CDS
CTCATGCTTCAAATGGCCAG+GGG 0.668271 5_2:-6479968 Msa0752730:CDS
GATCCAAAGATTACCAAGTG+AGG 0.673638 5_2:-6480276 Msa0752730:CDS
ATGAAGCCAAAGAAAGACCA+AGG 0.681190 5_2:-6478972 Msa0752730:intron
TGTAACATTCTTCTTTGACG+GGG 0.687811 5_2:+6479013 None:intergenic
GTACTAACAACAGCATTGTG+AGG 0.690491 5_2:-6478497 Msa0752730:CDS
GATAGTTCTTTGAAGATACG+AGG 0.692687 5_2:-6478393 Msa0752730:CDS
TGAAGCGATCGAGAATGCTG+GGG 0.704654 5_2:-6480644 Msa0752730:CDS
TTTGCTTTGCTATTTCCCTG+CGG 0.707457 5_2:-6480671 Msa0752730:CDS
TGTCACAACAATCCACAGGT+GGG 0.708247 5_2:+6479357 None:intergenic
ACATACATGAGAGAGAATTG+TGG 0.732943 5_2:-6480034 Msa0752730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATACATTAAGCATTGTT+TGG - chr5_2:6478493-6478512 Msa0752730:CDS 20.0%
!! ATAGATATTGCAATATGTTT+GGG + chr5_2:6479138-6479157 None:intergenic 20.0%
!! TTATAAGCAAACATTTCATA+AGG + chr5_2:6479997-6480016 None:intergenic 20.0%
!! CTTATAAAGTTGTTGAAAAA+GGG - chr5_2:6480010-6480029 Msa0752730:CDS 20.0%
!! TATTCCTACTATAGATAATA+AGG - chr5_2:6480065-6480084 Msa0752730:CDS 20.0%
!! ATTCCTACTATAGATAATAA+GGG - chr5_2:6480066-6480085 Msa0752730:CDS 20.0%
!! TGTATATATGTATATATAGC+AGG + chr5_2:6480347-6480366 None:intergenic 20.0%
!!! TCAGTTAGAAAAAAAGTATA+TGG - chr5_2:6480603-6480622 Msa0752730:CDS 20.0%
! ATTGTTTGGATTCAAAAGAT+TGG - chr5_2:6478507-6478526 Msa0752730:CDS 25.0%
!! AATTGCTTGCAAATTTCTTT+TGG + chr5_2:6478764-6478783 None:intergenic 25.0%
! AAAAGAAATTTGCAAGCAAT+TGG - chr5_2:6478763-6478782 Msa0752730:CDS 25.0%
! TTGTTGAAGAAATTGATGAA+TGG - chr5_2:6478893-6478912 Msa0752730:intron 25.0%
! GAAATTGATGAATGGTAATT+CGG - chr5_2:6478901-6478920 Msa0752730:intron 25.0%
! TCACAAAAACAGTAATGATA+AGG - chr5_2:6478934-6478953 Msa0752730:intron 25.0%
! AGGAATTTAAGGAAATTCAA+TGG - chr5_2:6479026-6479045 Msa0752730:CDS 25.0%
! TTTAAGGAAATTCAATGGTT+AGG - chr5_2:6479031-6479050 Msa0752730:CDS 25.0%
! CATAGATATTGCAATATGTT+TGG + chr5_2:6479139-6479158 None:intergenic 25.0%
! AACATATTGCAATATCTATG+TGG - chr5_2:6479139-6479158 Msa0752730:CDS 25.0%
!!! TATTTTCAAGCAAAAGTTGT+TGG - chr5_2:6479403-6479422 Msa0752730:CDS 25.0%
! AAGAGATTAACCCTATAATT+TGG - chr5_2:6479503-6479522 Msa0752730:CDS 25.0%
! TGAAAATGATTATCCTCAAA+AGG + chr5_2:6479655-6479674 None:intergenic 25.0%
!! TTTGAGGATAATCATTTTCA+AGG - chr5_2:6479655-6479674 Msa0752730:CDS 25.0%
!! TTGAGGATAATCATTTTCAA+GGG - chr5_2:6479656-6479675 Msa0752730:CDS 25.0%
!! ATCAAACTCACAAGATATTT+TGG + chr5_2:6479739-6479758 None:intergenic 25.0%
! TGAGTTTGATCAAAAAATGT+TGG - chr5_2:6479749-6479768 Msa0752730:CDS 25.0%
! GCTTATAAAGTTGTTGAAAA+AGG - chr5_2:6480009-6480028 Msa0752730:CDS 25.0%
!!! ATCAGAAATTGATTTTGGAT+AGG + chr5_2:6480202-6480221 None:intergenic 25.0%
! ATTGTAACATTCTTCTTTGA+CGG + chr5_2:6480252-6480271 None:intergenic 25.0%
! CAATAAAATTAAACTGACCT+TGG + chr5_2:6480308-6480327 None:intergenic 25.0%
! TGTATTGAATTGATTGAAAC+AGG - chr5_2:6480381-6480400 Msa0752730:CDS 25.0%
! AGGAAATCCAATATTAACAA+AGG - chr5_2:6480549-6480568 Msa0752730:CDS 25.0%
! GGAAATCCAATATTAACAAA+GGG - chr5_2:6480550-6480569 Msa0752730:CDS 25.0%
! TTGATTCCCTTTGTTAATAT+TGG + chr5_2:6480559-6480578 None:intergenic 25.0%
!! GTTAGAAAAAAAGTATATGG+AGG - chr5_2:6480606-6480625 Msa0752730:CDS 25.0%
!!! CTTGTTTTCAGATTTAAACT+AGG + chr5_2:6480651-6480670 None:intergenic 25.0%
! TTTGTCTTTAACCTGTTAAA+TGG + chr5_2:6480684-6480703 None:intergenic 25.0%
TTGAAGACAGAACTACAAAA+TGG - chr5_2:6478417-6478436 Msa0752730:CDS 30.0%
CAAGAAAATATGATGTGGAA+TGG - chr5_2:6478690-6478709 Msa0752730:CDS 30.0%
!!! ATGATCCAAAACTTTTTCAG+TGG - chr5_2:6478714-6478733 Msa0752730:CDS 30.0%
! AATCTCCACTGAAAAAGTTT+TGG + chr5_2:6478722-6478741 None:intergenic 30.0%
!!! AAACTTTTTCAGTGGAGATT+TGG - chr5_2:6478722-6478741 Msa0752730:CDS 30.0%
GAGATTTGGCAAACAATATT+TGG - chr5_2:6478736-6478755 Msa0752730:CDS 30.0%
! ATTCTTTGAAGAAGTGAGTT+TGG + chr5_2:6478852-6478871 None:intergenic 30.0%
!! ACAGTAATGATAAGGTATTG+TGG - chr5_2:6478942-6478961 Msa0752730:intron 30.0%
GATCCTAACAAAACACTAAT+TGG + chr5_2:6478968-6478987 None:intergenic 30.0%
GTGAGAAAGTGATAACAAAT+TGG + chr5_2:6479116-6479135 None:intergenic 30.0%
! GTTTCCATGGTAAATGTTTT+TGG + chr5_2:6479335-6479354 None:intergenic 30.0%
! GCTTTTAGTAGGATATTACA+AGG + chr5_2:6479364-6479383 None:intergenic 30.0%
!!! ATATGTTTTGAGTTGTTGAC+AGG - chr5_2:6479622-6479641 Msa0752730:CDS 30.0%
!!! TATGTTTTGAGTTGTTGACA+GGG - chr5_2:6479623-6479642 Msa0752730:CDS 30.0%
AGGATTGATCAAAACATACT+CGG + chr5_2:6479857-6479876 None:intergenic 30.0%
! TTTTCTCAAGAGTTTTCCTA+TGG - chr5_2:6479936-6479955 Msa0752730:CDS 30.0%
ATATTATATGTCCTCTCCAT+AGG + chr5_2:6479955-6479974 None:intergenic 30.0%
CTACCCTTATTATCTATAGT+AGG + chr5_2:6480072-6480091 None:intergenic 30.0%
TGCATCCATAATTAAAGATC+TGG - chr5_2:6480173-6480192 Msa0752730:CDS 30.0%
GGTTACCAGATCTTTAATTA+TGG + chr5_2:6480181-6480200 None:intergenic 30.0%
!! GTGTCATCAGAAATTGATTT+TGG + chr5_2:6480207-6480226 None:intergenic 30.0%
TTGTAACATTCTTCTTTGAC+GGG + chr5_2:6480251-6480270 None:intergenic 30.0%
!! GATTTTGTTGCTTGCAATTT+AGG + chr5_2:6480411-6480430 None:intergenic 30.0%
TTAACCTGTTAAATGGTGAT+TGG + chr5_2:6480677-6480696 None:intergenic 30.0%
! AACTATCCATATCTGAAACA+TGG + chr5_2:6480854-6480873 None:intergenic 30.0%
TTCTTACATAAGGACTCAAA+GGG + chr5_2:6480983-6481002 None:intergenic 30.0%
CCATATGTTGTTCTTACATA+AGG + chr5_2:6480993-6481012 None:intergenic 30.0%
CCTTATGTAAGAACAACATA+TGG - chr5_2:6480990-6481009 Msa0752730:CDS 30.0%
! TTGGATTCAAAAGATTGGTC+AGG - chr5_2:6478512-6478531 Msa0752730:CDS 35.0%
! TGGATTCAAAAGATTGGTCA+GGG - chr5_2:6478513-6478532 Msa0752730:CDS 35.0%
GCTTGCAAGAAAATATGATG+TGG - chr5_2:6478685-6478704 Msa0752730:CDS 35.0%
!! TGGCCAATTAGTGTTTTGTT+AGG - chr5_2:6478962-6478981 Msa0752730:intron 35.0%
! GAAAGAGTTTTCTCTTGTGA+CGG - chr5_2:6479180-6479199 Msa0752730:CDS 35.0%
!! AGTTGATGAACAAGGATTTG+TGG - chr5_2:6479203-6479222 Msa0752730:CDS 35.0%
ACATACATGAGAGAGAATTG+TGG - chr5_2:6479226-6479245 Msa0752730:CDS 35.0%
! GATTTTGTTCTCTATACCTG+TGG - chr5_2:6479261-6479280 Msa0752730:CDS 35.0%
!!! CATGGTAAATGTTTTTGGAG+TGG + chr5_2:6479330-6479349 None:intergenic 35.0%
CACTCCAAAAACATTTACCA+TGG - chr5_2:6479328-6479347 Msa0752730:CDS 35.0%
GTAGGATATTACAAGGACTA+AGG + chr5_2:6479357-6479376 None:intergenic 35.0%
!!! GTGAGTTTCTAGCTTTTAGT+AGG + chr5_2:6479375-6479394 None:intergenic 35.0%
AAAGCTAGAAACTCACAAGA+AGG - chr5_2:6479379-6479398 Msa0752730:CDS 35.0%
CAAGCAAAAGTTGTTGGATT+TGG - chr5_2:6479409-6479428 Msa0752730:CDS 35.0%
GGATTGTCATCTGTTGAAAA+TGG - chr5_2:6479430-6479449 Msa0752730:CDS 35.0%
! AATTGGATTATGTGTTGGTG+AGG + chr5_2:6479479-6479498 None:intergenic 35.0%
TCACCAACACATAATCCAAT+TGG - chr5_2:6479478-6479497 Msa0752730:CDS 35.0%
TCACCAATTGGATTATGTGT+TGG + chr5_2:6479484-6479503 None:intergenic 35.0%
CAGGTGCATACCAAATTATA+GGG + chr5_2:6479516-6479535 None:intergenic 35.0%
TCAGGTGCATACCAAATTAT+AGG + chr5_2:6479517-6479536 None:intergenic 35.0%
TATAATTTGGTATGCACCTG+AGG - chr5_2:6479516-6479535 Msa0752730:CDS 35.0%
TTTCTCTGAGTACTTACAAG+AGG + chr5_2:6479578-6479597 None:intergenic 35.0%
CATACCAAAGCTATATGCAT+CGG + chr5_2:6479602-6479621 None:intergenic 35.0%
ACCAACCAAAATATCAAAGC+AGG - chr5_2:6479685-6479704 Msa0752730:CDS 35.0%
!!! TCCTGCTTTGATATTTTGGT+TGG + chr5_2:6479689-6479708 None:intergenic 35.0%
! TCTCTCCTGCTTTGATATTT+TGG + chr5_2:6479693-6479712 None:intergenic 35.0%
AAAATATCAAAGCAGGAGAG+AGG - chr5_2:6479692-6479711 Msa0752730:CDS 35.0%
CTACAAATAGATGAGAAACC+AGG + chr5_2:6479796-6479815 None:intergenic 35.0%
CTGGTTTCTCATCTATTTGT+AGG - chr5_2:6479794-6479813 Msa0752730:CDS 35.0%
! AGTTCAAGCTGATTTAGTTC+AGG + chr5_2:6479877-6479896 None:intergenic 35.0%
TGTAACATTCTTCTTTGACG+GGG + chr5_2:6480250-6480269 None:intergenic 35.0%
! TTCTTTGGCTTCATTTGTGA+TGG + chr5_2:6480282-6480301 None:intergenic 35.0%
TCATTACCACCAACATTATC+TGG - chr5_2:6480429-6480448 Msa0752730:CDS 35.0%
AACTTGATGTCAATGATGAC+TGG + chr5_2:6480482-6480501 None:intergenic 35.0%
CTATAACCCCAGTTAAAAGA+AGG - chr5_2:6480502-6480521 Msa0752730:CDS 35.0%
! TTGACACGTCTTATTCTTGT+TGG + chr5_2:6480741-6480760 None:intergenic 35.0%
! GATAGTTCTTTGAAGATACG+AGG - chr5_2:6480867-6480886 Msa0752730:CDS 35.0%
GTTCTTACATAAGGACTCAA+AGG + chr5_2:6480984-6481003 None:intergenic 35.0%
!! TGGAAGTCTAAAAGCGCTTA+TGG - chr5_2:6478286-6478305 Msa0752730:CDS 40.0%
ATGCTAGAGTGAATATGTCG+TGG + chr5_2:6478358-6478377 None:intergenic 40.0%
ACACAATCGCAGATGAGATA+AGG - chr5_2:6478381-6478400 Msa0752730:CDS 40.0%
! TCTCTAAGAGGACATTCAAG+AGG + chr5_2:6478446-6478465 None:intergenic 40.0%
! CTTTCCAGGGTATTCAAAGA+AGG - chr5_2:6478467-6478486 Msa0752730:CDS 40.0%
TTCACCTTCTTTGAATACCC+TGG + chr5_2:6478474-6478493 None:intergenic 40.0%
!! TTTGCTTTGCTATTTCCCTG+CGG - chr5_2:6478589-6478608 Msa0752730:CDS 40.0%
AGAATGCTTGGAGAGAAGAT+AGG - chr5_2:6478786-6478805 Msa0752730:CDS 40.0%
! GAAGCACTCAAGGAGAAAAA+AGG - chr5_2:6478821-6478840 Msa0752730:CDS 40.0%
GATCCAAAGATTACCAAGTG+AGG - chr5_2:6478984-6479003 Msa0752730:CDS 40.0%
! TCTCCTCACTTGGTAATCTT+TGG + chr5_2:6478990-6479009 None:intergenic 40.0%
TTACCAAGTGAGGAGAAGAT+TGG - chr5_2:6478994-6479013 Msa0752730:CDS 40.0%
TACCAAGTGAGGAGAAGATT+GGG - chr5_2:6478995-6479014 Msa0752730:CDS 40.0%
! TAGGACAAAGTTTTGCTCTG+AGG - chr5_2:6479050-6479069 Msa0752730:CDS 40.0%
!!! TTTGCTCTGAGGCATTTTCT+TGG - chr5_2:6479061-6479080 Msa0752730:CDS 40.0%
!!! TTGCTCTGAGGCATTTTCTT+GGG - chr5_2:6479062-6479081 Msa0752730:CDS 40.0%
GAGAACAAAATCTGTCTCCT+TGG + chr5_2:6479253-6479272 None:intergenic 40.0%
TTTGTTCTCTATACCTGTGG+TGG - chr5_2:6479264-6479283 Msa0752730:CDS 40.0%
GGTTGATCTCATGCTTCAAA+TGG - chr5_2:6479285-6479304 Msa0752730:CDS 40.0%
TACAAGGACTAAGGTTTCCA+TGG + chr5_2:6479348-6479367 None:intergenic 40.0%
GGGTTAATCTCTTCACCAAT+TGG + chr5_2:6479496-6479515 None:intergenic 40.0%
! TCTAGCTCAGTTAAAACCTC+AGG + chr5_2:6479535-6479554 None:intergenic 40.0%
AAAGCCGATGCATATAGCTT+TGG - chr5_2:6479595-6479614 Msa0752730:CDS 40.0%
! GACAGGGAAAATTCCTTTTG+AGG - chr5_2:6479639-6479658 Msa0752730:CDS 40.0%
!!! AGATATTTTGGCGAGCGATA+TGG + chr5_2:6479727-6479746 None:intergenic 40.0%
TCAATGTCACAACAATCCAC+AGG + chr5_2:6479910-6479929 None:intergenic 40.0%
! GTGGATTGTTGTGACATTGA+AGG - chr5_2:6479910-6479929 Msa0752730:CDS 40.0%
! TCAAGAGTTTTCCTATGGAG+AGG - chr5_2:6479941-6479960 Msa0752730:CDS 40.0%
CATCTTCACAAACTGTGCTT+TGG + chr5_2:6480149-6480168 None:intergenic 40.0%
ATGAAGCCAAAGAAAGACCA+AGG - chr5_2:6480288-6480307 Msa0752730:CDS 40.0%
! AACTGACCTTGGTCTTTCTT+TGG + chr5_2:6480297-6480316 None:intergenic 40.0%
TACCACCAACATTATCTGGT+CGG - chr5_2:6480433-6480452 Msa0752730:CDS 40.0%
TCGGGTTTCAAGGACAAATA+AGG - chr5_2:6480452-6480471 Msa0752730:CDS 40.0%
!! TTGGAGGCCTTCTTTTAACT+GGG + chr5_2:6480512-6480531 None:intergenic 40.0%
! TTTGGAGGCCTTCTTTTAAC+TGG + chr5_2:6480513-6480532 None:intergenic 40.0%
!!! TCAGATTCTGAGACTTTTGG+AGG + chr5_2:6480528-6480547 None:intergenic 40.0%
!!! CTCTCAGATTCTGAGACTTT+TGG + chr5_2:6480531-6480550 None:intergenic 40.0%
CAAAAGTCTCAGAATCTGAG+AGG - chr5_2:6480529-6480548 Msa0752730:CDS 40.0%
GAGACCAATCACCATTTAAC+AGG - chr5_2:6480670-6480689 Msa0752730:CDS 40.0%
GTACTAACAACAGCATTGTG+AGG - chr5_2:6480763-6480782 Msa0752730:CDS 40.0%
AGCATTGTGAGGATGCAAAA+AGG - chr5_2:6480774-6480793 Msa0752730:CDS 40.0%
GCATTGTGAGGATGCAAAAA+GGG - chr5_2:6480775-6480794 Msa0752730:CDS 40.0%
TATGACCTTGAAGGACTCAT+TGG + chr5_2:6480816-6480835 None:intergenic 40.0%
ATGCTGCCATGTTTCAGATA+TGG - chr5_2:6480845-6480864 Msa0752730:CDS 40.0%
CTCAAAGGACTCAATGGAAA+TGG + chr5_2:6480903-6480922 None:intergenic 40.0%
TATGGACTCAAAGGACTCAA+TGG + chr5_2:6480909-6480928 None:intergenic 40.0%
TTGCTCACATATGGACTCAA+AGG + chr5_2:6480918-6480937 None:intergenic 40.0%
GTCCATATGTGAGCAATCAA+AGG - chr5_2:6480922-6480941 Msa0752730:CDS 40.0%
GTCCTTTGATTGCTCACATA+TGG + chr5_2:6480927-6480946 None:intergenic 40.0%
AGCAATCAAAGGACTAGAAG+AGG - chr5_2:6480933-6480952 Msa0752730:CDS 40.0%
TCCTCTTAGAGAGCTTTCCA+GGG - chr5_2:6478454-6478473 Msa0752730:CDS 45.0%
ATTGAAGCGATCGAGAATGC+TGG - chr5_2:6478614-6478633 Msa0752730:CDS 45.0%
TTGAAGCGATCGAGAATGCT+GGG - chr5_2:6478615-6478634 Msa0752730:CDS 45.0%
GCAAGCAATTGGAGAATGCT+TGG - chr5_2:6478774-6478793 Msa0752730:CDS 45.0%
ATGCTTGGAGAGAAGATAGG+TGG - chr5_2:6478789-6478808 Msa0752730:CDS 45.0%
TTCCCAATCTTCTCCTCACT+TGG + chr5_2:6479000-6479019 None:intergenic 45.0%
AAGTGAGGAGAAGATTGGGA+AGG - chr5_2:6478999-6479018 Msa0752730:CDS 45.0%
AGTGAGGAGAAGATTGGGAA+GGG - chr5_2:6479000-6479019 Msa0752730:CDS 45.0%
!!! TGCTCTGAGGCATTTTCTTG+GGG - chr5_2:6479063-6479082 Msa0752730:CDS 45.0%
!! TGTGACGGAGTTGATGAACA+AGG - chr5_2:6479195-6479214 Msa0752730:CDS 45.0%
TGAGAGAGAATTGTGGTCCA+AGG - chr5_2:6479233-6479252 Msa0752730:CDS 45.0%
ATCTCATGCTTCAAATGGCC+AGG - chr5_2:6479290-6479309 Msa0752730:CDS 45.0%
TCTCATGCTTCAAATGGCCA+GGG - chr5_2:6479291-6479310 Msa0752730:CDS 45.0%
TGTCACAACAATCCACAGGT+GGG + chr5_2:6479906-6479925 None:intergenic 45.0%
ATGTCACAACAATCCACAGG+TGG + chr5_2:6479907-6479926 None:intergenic 45.0%
TAGTGAGTCTGAGCCAACAA+AGG - chr5_2:6480089-6480108 Msa0752730:CDS 45.0%
ACAGTAGCCACATCCTTTGT+TGG + chr5_2:6480105-6480124 None:intergenic 45.0%
ACCACCAACATTATCTGGTC+GGG - chr5_2:6480434-6480453 Msa0752730:CDS 45.0%
GAAACCCGACCAGATAATGT+TGG + chr5_2:6480441-6480460 None:intergenic 45.0%
CATTATCTGGTCGGGTTTCA+AGG - chr5_2:6480442-6480461 Msa0752730:CDS 45.0%
!! TGGAGGCCTTCTTTTAACTG+GGG + chr5_2:6480511-6480530 None:intergenic 45.0%
CTTCTGGTGTATGACCTTGA+AGG + chr5_2:6480825-6480844 None:intergenic 45.0%
TGAAACATGGCAGCATCTTC+TGG + chr5_2:6480841-6480860 None:intergenic 45.0%
CTCAAAGGGCTCAATGCTAA+TGG + chr5_2:6480969-6480988 None:intergenic 45.0%
CAAGAACCTCACCAATGTGC+CGG + chr5_2:6478268-6478287 None:intergenic 50.0%
GTCCTCTTAGAGAGCTTTCC+AGG - chr5_2:6478453-6478472 Msa0752730:CDS 50.0%
ACCCTGGAAAGCTCTCTAAG+AGG + chr5_2:6478458-6478477 None:intergenic 50.0%
CGATCGCTTCAATAACCGCA+GGG + chr5_2:6478607-6478626 None:intergenic 50.0%
TCGATCGCTTCAATAACCGC+AGG + chr5_2:6478608-6478627 None:intergenic 50.0%
TGAAGCGATCGAGAATGCTG+GGG - chr5_2:6478616-6478635 Msa0752730:CDS 50.0%
AGAATGCTGGGGAGATGTCA+GGG - chr5_2:6478627-6478646 Msa0752730:CDS 50.0%
CTTGGAGAGAAGATAGGTGG+AGG - chr5_2:6478792-6478811 Msa0752730:CDS 50.0%
!! GAGGCTCATAGAAGCACTCA+AGG - chr5_2:6478811-6478830 Msa0752730:CDS 50.0%
GTGAGGAGAAGATTGGGAAG+GGG - chr5_2:6479001-6479020 Msa0752730:CDS 50.0%
TGAGGAGAAGATTGGGAAGG+GGG - chr5_2:6479002-6479021 Msa0752730:CDS 50.0%
GGGAAGGGGGAAGGAATTTA+AGG - chr5_2:6479015-6479034 Msa0752730:CDS 50.0%
AGCATGAGATCAACCACCAC+AGG + chr5_2:6479280-6479299 None:intergenic 50.0%
CTCATGCTTCAAATGGCCAG+GGG - chr5_2:6479292-6479311 Msa0752730:CDS 50.0%
GTGGAGATATTCAATGCCCC+TGG + chr5_2:6479311-6479330 None:intergenic 50.0%
GGCGAGCGATATGGAAACAA+AGG + chr5_2:6479718-6479737 None:intergenic 50.0%
TGAACTTCAGAACCCACCTG+TGG - chr5_2:6479891-6479910 Msa0752730:CDS 50.0%
GTCTGAGCCAACAAAGGATG+TGG - chr5_2:6480095-6480114 Msa0752730:CDS 50.0%
ACCCGACCAGATAATGTTGG+TGG + chr5_2:6480438-6480457 None:intergenic 50.0%
GACCTTGAAGGACTCATTGG+CGG + chr5_2:6480813-6480832 None:intergenic 50.0%
ATGGAACAGCTCCGGCACAT+TGG - chr5_2:6478254-6478273 Msa0752730:CDS 55.0%
CGGCACATTGGTGAGGTTCT+TGG - chr5_2:6478266-6478285 Msa0752730:CDS 55.0%
GAGAATGCTGGGGAGATGTC+AGG - chr5_2:6478626-6478645 Msa0752730:CDS 55.0%
GGAGATGTCAGGGTTGGATC+AGG - chr5_2:6478637-6478656 Msa0752730:CDS 55.0%
GAGAAGATTGGGAAGGGGGA+AGG - chr5_2:6479006-6479025 Msa0752730:CDS 55.0%
GAGAAACCAGGACGCTGAGA+CGG + chr5_2:6479784-6479803 None:intergenic 55.0%
CACCGCCAATGAGTCCTTCA+AGG - chr5_2:6480808-6480827 Msa0752730:CDS 55.0%
ACAGCTCCGGCACATTGGTG+AGG - chr5_2:6478259-6478278 Msa0752730:CDS 60.0%
TGCTGGGGAGATGTCAGGGT+TGG - chr5_2:6478631-6478650 Msa0752730:CDS 60.0%
ACTGATCCGTCTCAGCGTCC+TGG - chr5_2:6479775-6479794 Msa0752730:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 6478254 6481028 6478254 ID=Msa0752730;Name=Msa0752730
chr5_2 mRNA 6478254 6481028 6478254 ID=Msa0752730-mRNA-1;Parent=Msa0752730;Name=Msa0752730-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|893
chr5_2 exon 6478973 6481028 6478973 ID=Msa0752730-mRNA-1:exon:2764;Parent=Msa0752730-mRNA-1
chr5_2 exon 6478254 6478879 6478254 ID=Msa0752730-mRNA-1:exon:2763;Parent=Msa0752730-mRNA-1
chr5_2 CDS 6478973 6481028 6478973 ID=Msa0752730-mRNA-1:cds;Parent=Msa0752730-mRNA-1
chr5_2 CDS 6478254 6478879 6478254 ID=Msa0752730-mRNA-1:cds;Parent=Msa0752730-mRNA-1
Gene Sequence

>Msa0752730

ATGGAACAGCTCCGGCACATTGGTGAGGTTCTTGGAAGTCTAAAAGCGCTTATGGTTTTAAGCGACGAAATTCAAATCAATCAACGACAATGTTGTTTGCTCCACGACATATTCACTCTAGCATTCGACACAATCGCAGATGAGATAAGGCAGAATCTGAAGCTTGAAGACAGAACTACAAAATGGAAGCCTCTTGAATGTCCTCTTAGAGAGCTTTCCAGGGTATTCAAAGAAGGTGAATTATACATTAAGCATTGTTTGGATTCAAAAGATTGGTCAGGGAAAGCACTCACACTTTCTCAGAACAGAGAATGTGTTGAGTTTCACATTCATAATTTGCTTTGCTATTTCCCTGCGGTTATTGAAGCGATCGAGAATGCTGGGGAGATGTCAGGGTTGGATCAGGATGAGAATTCGAAGAAGAAATTGATGCTTGCAAGAAAATATGATGTGGAATGGAATGATCCAAAACTTTTTCAGTGGAGATTTGGCAAACAATATTTGGTTCCAAAAGAAATTTGCAAGCAATTGGAGAATGCTTGGAGAGAAGATAGGTGGAGGCTCATAGAAGCACTCAAGGAGAAAAAAGGTTCTTCCAAACTCACTTCTTCAAAGAATGATCAACATTTTGCAGATATGTTGTTGAAGAAATTGATGAATGGTAATTCGGATAAAACTAATCACAAAAACAGTAATGATAAGGTATTGTGGCCAATTAGTGTTTTGTTAGGATCCAAAGATTACCAAGTGAGGAGAAGATTGGGAAGGGGGAAGGAATTTAAGGAAATTCAATGGTTAGGACAAAGTTTTGCTCTGAGGCATTTTCTTGGGGAGAGAGAAGCTTATGAAAATGAGATTTCCAATTTGTTATCACTTTCTCACCCAAACATATTGCAATATCTATGTGGTTTTTATGATGAAGAGAAGAAAGAGTTTTCTCTTGTGACGGAGTTGATGAACAAGGATTTGTGGACATACATGAGAGAGAATTGTGGTCCAAGGAGACAGATTTTGTTCTCTATACCTGTGGTGGTTGATCTCATGCTTCAAATGGCCAGGGGCATTGAATATCTCCACTCCAAAAACATTTACCATGGAAACCTTAGTCCTTGTAATATCCTACTAAAAGCTAGAAACTCACAAGAAGGTTATTTTCAAGCAAAAGTTGTTGGATTTGGATTGTCATCTGTTGAAAATGGTGAAATTTATAATGCTTCTAGATCCTCACCAACACATAATCCAATTGGTGAAGAGATTAACCCTATAATTTGGTATGCACCTGAGGTTTTAACTGAGCTAGAACAAATGAAAAATGCTTCTACCTCTTGTAAGTACTCAGAGAAAGCCGATGCATATAGCTTTGGTATGATATGTTTTGAGTTGTTGACAGGGAAAATTCCTTTTGAGGATAATCATTTTCAAGGGATTAGAACCAACCAAAATATCAAAGCAGGAGAGAGGCCTTTGTTTCCATATCGCTCGCCAAAATATCTTGTGAGTTTGATCAAAAAATGTTGGCAAACTGATCCGTCTCAGCGTCCTGGTTTCTCATCTATTTGTAGGATTTTGCGCTACATCAAAAAGTTTCTTTCAATGAACACCGAGTATGTTTTGATCAATCCTGAACTAAATCAGCTTGAACTTCAGAACCCACCTGTGGATTGTTGTGACATTGAAGGAATTTTTCTCAAGAGTTTTCCTATGGAGAGGACATATAATATGTCTTGTGTTTCACAAATACCTTATGAAATGTTTGCTTATAAAGTTGTTGAAAAAGGGAAGATTAATAATCAGAATCTGAGTAATAATACTATTCCTACTATAGATAATAAGGGTAGTGAGTCTGAGCCAACAAAGGATGTGGCTACTGTTCGTAGCGAAGAAAGTGATGACCAAAGCACAGTTTGTGAAGATGTAGATGCATCCATAATTAAAGATCTGGTAACCTATCCAAAATCAATTTCTGATGACACACAGTCTATTTTTTATGATACCCCGTCAAAGAAGAATGTTACAATAAAGAAACCATCACAAATGAAGCCAAAGAAAGACCAAGGAACGCCTAAATTGCAAGCAACAAAATCATTACCACCAACATTATCTGGTCGGGTTTCAAGGACAAATAAGGCGAACCAGTCATCATTGACATCAAGTTCTATAACCCCAGTTAAAAGAAGGCCTCCAAAAGTCTCAGAATCTGAGAGGAAATCCAATATTAACAAAGGGAATCAATCAGCATCAGTGTCAACTTCAAGCTCAGTTAGAAAAAAAGTATATGGAGGCAATGTCTCAGATTCTAAGCCTAGTTTAAATCTGAAAACAAGAGACCAATCACCATTTAACAGGTTAAAGACAAAAAAATCACAACTAACGATGAATTCTTCTTCGAGTCCAACAAGAATAAGACGTGTCAATAGTACTAACAACAGCATTGTGAGGATGCAAAAAGGGTTCATTTCAACACCGCCAATGAGTCCTTCAAGGTCATACACCAGAAGATGCTGCCATGTTTCAGATATGGATAGTTCTTTGAAGATACGAGGTAGATTGTCACCATTTCCATTGAGTCCTTTGAGTCCATATGTGAGCAATCAAAGGACTAGAAGAGGAAGCTTGTCACCATTAGCATTGAGCCCTTTGAGTCCTTATGTAAGAACAACATATGGTCATGTCTCAGATTAA
Protein sequence

>Msa0752730

MEQLRHIGEVLGSLKALMVLSDEIQINQRQCCLLHDIFTLAFDTIADEIRQNLKLEDRTTKWKPLECPLRELSRVFKEGELYIKHCLDSKDWSGKALTLSQNRECVEFHIHNLLCYFPAVIEAIENAGEMSGLDQDENSKKKLMLARKYDVEWNDPKLFQWRFGKQYLVPKEICKQLENAWREDRWRLIEALKEKKGSSKLTSSKNDQHFADMLLKKLMNGNSDKTNHKNSNDKVLWPISVLLGSKDYQVRRRLGRGKEFKEIQWLGQSFALRHFLGEREAYENEISNLLSLSHPNILQYLCGFYDEEKKEFSLVTELMNKDLWTYMRENCGPRRQILFSIPVVVDLMLQMARGIEYLHSKNIYHGNLSPCNILLKARNSQEGYFQAKVVGFGLSSVENGEIYNASRSSPTHNPIGEEINPIIWYAPEVLTELEQMKNASTSCKYSEKADAYSFGMICFELLTGKIPFEDNHFQGIRTNQNIKAGERPLFPYRSPKYLVSLIKKCWQTDPSQRPGFSSICRILRYIKKFLSMNTEYVLINPELNQLELQNPPVDCCDIEGIFLKSFPMERTYNMSCVSQIPYEMFAYKVVEKGKINNQNLSNNTIPTIDNKGSESEPTKDVATVRSEESDDQSTVCEDVDASIIKDLVTYPKSISDDTQSIFYDTPSKKNVTIKKPSQMKPKKDQGTPKLQATKSLPPTLSGRVSRTNKANQSSLTSSSITPVKRRPPKVSESERKSNINKGNQSASVSTSSSVRKKVYGGNVSDSKPSLNLKTRDQSPFNRLKTKKSQLTMNSSSSPTRIRRVNSTNNSIVRMQKGFISTPPMSPSRSYTRRCCHVSDMDSSLKIRGRLSPFPLSPLSPYVSNQRTRRGSLSPLALSPLSPYVRTTYGHVSD*