AlfalfaGEDB | Msa0753030

M. sativa cultivar zhongmu-4 / Msa0753030

KEGG: K13412
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010119 GO:0010359 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903959
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0753030	sp\|Q6I587\|CDPKF_ORYSJ	73.554	484	88	3	53	514	75	540	0.0	754

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0753030	G7KAQ8	91.418	536	6	3	1	515	1	517	0.0	962

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0753030	MtrunA17_Chr5g0397101	91.418	536	6	3	1	515	1	517	962
Msa0753030	MtrunA17_Chr8g0387671	79.151	518	60	5	27	515	18	516	823
Msa0753030	MtrunA17_Chr0c02g0489761	67.002	497	119	4	45	514	50	528	693
Msa0753030	MtrunA17_Chr5g0441691	62.570	537	159	5	1	514	1	518	688
Msa0753030	MtrunA17_Chr3g0098951	62.764	521	142	6	18	505	30	531	667
Msa0753030	MtrunA17_Chr5g0444171	64.729	499	129	6	35	504	52	532	665
Msa0753030	MtrunA17_Chr4g0074811	59.347	551	161	6	1	506	1	533	662
Msa0753030	MtrunA17_Chr1g0157131	63.312	477	135	4	53	507	59	517	614
Msa0753030	MtrunA17_Chr1g0170281	59.499	479	153	4	56	512	106	565	592
Msa0753030	MtrunA17_Chr5g0448721	59.958	472	148	4	56	505	84	536	582

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0753030	AT4G23650.1	73.085	483	90	3	54	514	64	528	746
Msa0753030	AT5G12180.1	64.971	511	133	4	27	515	38	524	692
Msa0753030	AT5G19360.1	64.971	511	133	4	27	515	33	519	687
Msa0753030	AT3G20410.2	59.820	555	152	8	1	506	1	533	658
Msa0753030	AT3G20410.1	59.820	555	152	8	1	506	1	533	658
Msa0753030	AT1G50700.1	59.889	541	156	8	1	506	1	515	648
Msa0753030	AT4G04720.2	59.065	535	167	7	5	505	4	520	641
Msa0753030	AT4G04720.1	59.065	535	167	7	5	505	4	520	641
Msa0753030	AT1G50700.2	57.219	561	165	8	1	506	1	541	634
Msa0753030	AT4G21940.1	59.029	515	168	4	15	504	45	541	629

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Find 147 sgRNAs with CRISPR-Local

Find 383 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTGGTCTCCCCAAACT+AGG	0.529317	5_2:-6715440	Msa0753030:CDS
AAATCTTAGTACCTAGTTTG+GGG	0.615766	5_2:+6715429	None:intergenic
AACAATCAATACGAATTACA+AGG	0.595886	5_2:+6713853	None:intergenic
AACCAGAATGAAACAATTCA+GGG	0.543661	5_2:-6715841	Msa0753030:CDS
AACGGGGCAACGATTTGTGA+TGG	0.622134	5_2:+6713810	None:intergenic
AACTTATAAGTTGTTGGTCT+TGG	0.411704	5_2:-6713761	Msa0753030:three_prime_UTR
AACTTATAAGTTTGTAATTA+TGG	0.160394	5_2:+6713772	None:intergenic
AAGCTGATATTTGGAGTGCT+GGG	0.530045	5_2:-6717635	Msa0753030:CDS
AAGTGTGAGAAAGATCAGAA+AGG	0.650240	5_2:+6716973	None:intergenic
AATATTCAAAAGCTTTGTAT+AGG	0.371934	5_2:+6714774	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CGGAATATTGTTGAACTTAA+AGG	-	chr5_2:6714020-6714039	Msa0753030:intron	30.0%
CTAAGAAACAAGAAATTCTC+AGG	+	chr5_2:6714227-6714246	None:intergenic	30.0%
AGCTTAATTAAGTGTGTGTT+TGG	-	chr5_2:6714327-6714346	Msa0753030:intron	30.0%
TTGGTTTACAATGAGTTTGT+TGG	-	chr5_2:6714384-6714403	Msa0753030:CDS	30.0%
AAATGCGTCATTTCAAACAT+AGG	-	chr5_2:6715185-6715204	Msa0753030:intron	30.0%
GATGTATTCAAAGATCTTGT+TGG	-	chr5_2:6715277-6715296	Msa0753030:intron	30.0%
ATGTAGATTCATTTGTTTCC+TGG	-	chr5_2:6715491-6715510	Msa0753030:CDS	30.0%
TGTAGATTCATTTGTTTCCT+GGG	-	chr5_2:6715492-6715511	Msa0753030:CDS	30.0%
TGTGTATTAATGTGCAACTT+TGG	-	chr5_2:6715570-6715589	Msa0753030:intron	30.0%
CATCAATTGCTTAGTTAGTT+AGG	-	chr5_2:6715644-6715663	Msa0753030:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	6713642	6719365	6713642	ID=Msa0753030;Name=Msa0753030
chr5_2	mRNA	6713642	6719365	6713642	ID=Msa0753030-mRNA-1;Parent=Msa0753030;Name=Msa0753030-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0.75\|0.77\|1\|0.87\|0.77\|9\|365\|515
chr5_2	exon	6713642	6713953	6713642	ID=Msa0753030-mRNA-1:exon:2918;Parent=Msa0753030-mRNA-1
chr5_2	exon	6714058	6714194	6714058	ID=Msa0753030-mRNA-1:exon:2917;Parent=Msa0753030-mRNA-1
chr5_2	exon	6714344	6714443	6714344	ID=Msa0753030-mRNA-1:exon:2916;Parent=Msa0753030-mRNA-1
chr5_2	exon	6714755	6714882	6714755	ID=Msa0753030-mRNA-1:exon:2915;Parent=Msa0753030-mRNA-1
chr5_2	exon	6715397	6715564	6715397	ID=Msa0753030-mRNA-1:exon:2914;Parent=Msa0753030-mRNA-1
chr5_2	exon	6715809	6715921	6715809	ID=Msa0753030-mRNA-1:exon:2913;Parent=Msa0753030-mRNA-1
chr5_2	exon	6716798	6717004	6716798	ID=Msa0753030-mRNA-1:exon:2912;Parent=Msa0753030-mRNA-1
chr5_2	exon	6717589	6717732	6717589	ID=Msa0753030-mRNA-1:exon:2911;Parent=Msa0753030-mRNA-1
chr5_2	exon	6718762	6719365	6718762	ID=Msa0753030-mRNA-1:exon:2910;Parent=Msa0753030-mRNA-1
chr5_2	CDS	6718762	6719365	6718762	ID=Msa0753030-mRNA-1:cds;Parent=Msa0753030-mRNA-1
chr5_2	CDS	6717589	6717732	6717589	ID=Msa0753030-mRNA-1:cds;Parent=Msa0753030-mRNA-1
chr5_2	CDS	6716798	6717004	6716798	ID=Msa0753030-mRNA-1:cds;Parent=Msa0753030-mRNA-1
chr5_2	CDS	6715809	6715921	6715809	ID=Msa0753030-mRNA-1:cds;Parent=Msa0753030-mRNA-1
chr5_2	CDS	6715397	6715564	6715397	ID=Msa0753030-mRNA-1:cds;Parent=Msa0753030-mRNA-1
chr5_2	CDS	6714755	6714882	6714755	ID=Msa0753030-mRNA-1:cds;Parent=Msa0753030-mRNA-1
chr5_2	CDS	6714344	6714443	6714344	ID=Msa0753030-mRNA-1:cds;Parent=Msa0753030-mRNA-1
chr5_2	CDS	6714111	6714194	6714111	ID=Msa0753030-mRNA-1:cds;Parent=Msa0753030-mRNA-1
chr5_2	three_prime_UTR	6714058	6714110	6714058	ID=Msa0753030-mRNA-1:three_prime_utr;Parent=Msa0753030-mRNA-1
chr5_2	three_prime_UTR	6713642	6713953	6713642	ID=Msa0753030-mRNA-1:three_prime_utr;Parent=Msa0753030-mRNA-1

Gene Sequence

>Msa0753030

ATGGGCAACCGCATCAGCTCCAAACACAAACCCTCTTCCGATTCTTCTCAAACTCATTCCAAATCACAACCCTCACATTCCACTCCTACTTCCTTCCATCCTCCTAATCAAACCCCCAAACAATCCACTCACACCACCACTCCTTCCTCTTCCCCCCTCGGCCGCGTCTTAGGCAAGCCCATGGAAGACGTCCGATCAACCTACACCTTTGGCCGCGAACTCGGTCGCGGCCAATTCGGTGTAACCTACTTAGTCACACACAAACTCACCAAAGAACAATTCGCCTGCAAATCTATCGCCACTCGGAAACTCATCCGCCGCGATGATCTCGACGATATTCGCCGTGAAGTTCAGATCATGCATCATCTCACCGGTCACCGGAATATTGTTGAACTTAAAGGTGCTTATGAGGATCGTCATTCGGTTAATCTCATCATGGAGCTTTGTGCTGGTGGTGAGCTTTTTGATAGGATTATATCCAAAGGTCATTACTCTGAACGTGCTGCAGCTGAGCTGTGTCGCCAGATTGTCACTGTTGTTCATAATTGTCATACTATGGGTGTTATGCATAGAGATTTGAAGCCTGAGAATTTCTTGTTTCTTAAAGATGTATTCAAAGATCTTGTTGGGAGTGCATATTATGTTGCTCCTGAGGTGTTGCGTAGAAGTTATGGACCTGAAGCTGATATTTGGAGTGCTGGGATTATTTTGTACATTCTCCTCTCTGGTGTTCCTCCCTTCTGGGCAGTATATGGTACCTTTCTGATCTTTCTCACACTTTCGTCCTCTACTTTATGTGAAGAAAATGAACAAGGTATCTTTGATGCTATTCTTCGTGGACATCTTGATTTCGCATCTGATCCTTGGCCTAAAATATCGAGTATTGCTAAAGATCTGATCAAGAAGATGTTGCGAGCTGACCCTAAAGAACGAATTTCAGCTGTTGAAGTCCTTGACCATCCTTGGATGAAAGAAGATGGAGCATCTGATAAGCCTCTCGATATTGCTGTCTTAACCAGAATGAAACAATTCAGGGCAATGAACAAGCTAAAGAAAGTAGCTCTAAAGGTTATTGCTGAAAATCTTTCTGAAGAAGAAATTATAGGCTTGAAGGAAATGTTTAAATCCATGGATACAGATAACAGTGGAACAATCACTTTTGAAGAGTTGAAAGCTGGTCTCCCCAAACTAGGTACTAAGATTTCGGAGTCTGAAGTAAGGCAGTTGATGGAAGCGGCTGATGTTGACGGAAATGGAACCATTGATTATATTGAATTTATAACAGCTACCATGCACTTGAATAGAATGGAAAGAGAGGACCACCTATACAAAGCTTTTGAATATTTTGACAATGACAAGAGCGGGTACATTACAAAAGAAGAGTTGGAATCTGCCCTTACAAAGTACAACATGGGTGATGAGAAAACAATAAAGGAGATTATTGACGAAGTTGATTCAGACAATGATGGAAGAATTAACTATGAGGAGTTTGTAGCCATGATGAGGAAAGGCAATCCAGACTTGATAACCAACAAACGTCGCAAATAA

Protein sequence

>Msa0753030

MGNRISSKHKPSSDSSQTHSKSQPSHSTPTSFHPPNQTPKQSTHTTTPSSSPLGRVLGKPMEDVRSTYTFGRELGRGQFGVTYLVTHKLTKEQFACKSIATRKLIRRDDLDDIRREVQIMHHLTGHRNIVELKGAYEDRHSVNLIMELCAGGELFDRIISKGHYSERAAAELCRQIVTVVHNCHTMGVMHRDLKPENFLFLKDVFKDLVGSAYYVAPEVLRRSYGPEADIWSAGIILYILLSGVPPFWAVYGTFLIFLTLSSSTLCEENEQGIFDAILRGHLDFASDPWPKISSIAKDLIKKMLRADPKERISAVEVLDHPWMKEDGASDKPLDIAVLTRMKQFRAMNKLKKVALKVIAENLSEEEIIGLKEMFKSMDTDNSGTITFEELKAGLPKLGTKISESEVRQLMEAADVDGNGTIDYIEFITATMHLNRMEREDHLYKAFEYFDNDKSGYITKEELESALTKYNMGDEKTIKEIIDEVDSDNDGRINYEEFVAMMRKGNPDLITNKRRK*