Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754980 | RHN53807.1 | 81.522 | 92 | 17 | 0 | 25 | 116 | 2 | 93 | 1.48e-46 | 157 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754980 | A0A396HSW0 | 81.522 | 92 | 17 | 0 | 25 | 116 | 2 | 93 | 7.08e-47 | 157 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0754980 | TF | Tify |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754980 | MtrunA17_Chr5g0399851 | 81.522 | 92 | 17 | 0 | 25 | 116 | 2 | 93 | 1.36e-50 | 157 |
| Msa0754980 | MtrunA17_Chr5g0399861 | 66.379 | 116 | 33 | 1 | 1 | 116 | 1 | 110 | 9.65e-50 | 156 |
| Msa0754980 | MtrunA17_Chr5g0399881 | 65.517 | 116 | 34 | 1 | 1 | 116 | 1 | 110 | 8.46e-47 | 149 |
| Msa0754980 | MtrunA17_Chr2g0301131 | 37.895 | 95 | 45 | 3 | 41 | 125 | 109 | 199 | 8.58e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACAAGAGGTTTCAAATT+TGG | 0.130518 | 5_2:+8731568 | Msa0754980:CDS |
| CCATCCACACCTGTTATTTA+TGG | 0.168614 | 5_2:+8731977 | Msa0754980:CDS |
| AGAGGATGTAGTTCCTTTCT+TGG | 0.251859 | 5_2:-8731861 | None:intergenic |
| CTACAAGAGGTTTCAAATTT+GGG | 0.272831 | 5_2:+8731569 | Msa0754980:CDS |
| CATCATAAAATATTGTCATT+TGG | 0.312886 | 5_2:-8731778 | None:intergenic |
| ATCATAAAATATTGTCATTT+GGG | 0.325400 | 5_2:-8731777 | None:intergenic |
| TTTCAAATTTGGGACATTCC+AGG | 0.386089 | 5_2:+8731579 | Msa0754980:CDS |
| GGAGCTTGTTGAAAATTCAT+AGG | 0.431112 | 5_2:-8731536 | None:intergenic |
| GAATTTCTAGCAAGAAATGA+TGG | 0.446507 | 5_2:-8731932 | None:intergenic |
| TGGTACTTGAAGAGAATTGT+AGG | 0.475609 | 5_2:-8731957 | None:intergenic |
| TCCTCATCTCTTTCTACAAG+AGG | 0.490417 | 5_2:+8731556 | Msa0754980:CDS |
| AATGAAGGCTAATATTATCA+AGG | 0.546904 | 5_2:+8731739 | Msa0754980:CDS |
| AAGCATAGTTGTTATTGTTG+TGG | 0.572885 | 5_2:-8731889 | None:intergenic |
| ACCTCTTGTAGAAAGAGATG+AGG | 0.589295 | 5_2:-8731557 | None:intergenic |
| CCATAAATAACAGGTGTGGA+TGG | 0.595900 | 5_2:-8731977 | None:intergenic |
| TTTACCATAAATAACAGGTG+TGG | 0.596443 | 5_2:-8731981 | None:intergenic |
| AATTTCTAGCAAGAAATGAT+GGG | 0.637681 | 5_2:-8731931 | None:intergenic |
| TTCTAGCAAGAAATGATGGG+TGG | 0.652275 | 5_2:-8731928 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATGGTAAAAAAAATAAT+TGG | + | chr5_2:8731993-8732012 | Msa0754980:CDS | 10.0% |
| !!! | ATCATAAAATATTGTCATTT+GGG | - | chr5_2:8731780-8731799 | None:intergenic | 15.0% |
| !!! | AAATGACAATATTTTATGAT+GGG | + | chr5_2:8731780-8731799 | Msa0754980:CDS | 15.0% |
| !!! | TTTTTTTTACCATAAATAAC+AGG | - | chr5_2:8731989-8732008 | None:intergenic | 15.0% |
| !! | CATCATAAAATATTGTCATT+TGG | - | chr5_2:8731781-8731800 | None:intergenic | 20.0% |
| !!! | CAAATGACAATATTTTATGA+TGG | + | chr5_2:8731779-8731798 | Msa0754980:CDS | 20.0% |
| ! | AGTAGCTAAAAAAATGTTGA+TGG | - | chr5_2:8731639-8731658 | None:intergenic | 25.0% |
| ! | CATGATTCATGTTTATAACA+TGG | + | chr5_2:8731671-8731690 | Msa0754980:intron | 25.0% |
| !!! | GTTTTATTACAGTGTAATGA+AGG | + | chr5_2:8731724-8731743 | Msa0754980:intron | 25.0% |
| ! | AATGAAGGCTAATATTATCA+AGG | + | chr5_2:8731739-8731758 | Msa0754980:CDS | 25.0% |
| !!! | AATTTCTAGCAAGAAATGAT+GGG | - | chr5_2:8731934-8731953 | None:intergenic | 25.0% |
| TCTACAAGAGGTTTCAAATT+TGG | + | chr5_2:8731568-8731587 | Msa0754980:CDS | 30.0% | |
| CTACAAGAGGTTTCAAATTT+GGG | + | chr5_2:8731569-8731588 | Msa0754980:CDS | 30.0% | |
| ! | AAGCATAGTTGTTATTGTTG+TGG | - | chr5_2:8731892-8731911 | None:intergenic | 30.0% |
| !! | GAATTTCTAGCAAGAAATGA+TGG | - | chr5_2:8731935-8731954 | None:intergenic | 30.0% |
| TTTACCATAAATAACAGGTG+TGG | - | chr5_2:8731984-8732003 | None:intergenic | 30.0% | |
| !! | GGAGCTTGTTGAAAATTCAT+AGG | - | chr5_2:8731539-8731558 | None:intergenic | 35.0% |
| ! | TTTCAAATTTGGGACATTCC+AGG | + | chr5_2:8731579-8731598 | Msa0754980:CDS | 35.0% |
| TGAAAAAGTCAACGACATCA+TGG | + | chr5_2:8731832-8731851 | Msa0754980:CDS | 35.0% | |
| ! | ATCATGGTCTTTTCCAAGAA+AGG | + | chr5_2:8731848-8731867 | Msa0754980:CDS | 35.0% |
| !!! | GTTATTGTTGTGGTTTTGAG+AGG | - | chr5_2:8731882-8731901 | None:intergenic | 35.0% |
| TGGTACTTGAAGAGAATTGT+AGG | - | chr5_2:8731960-8731979 | None:intergenic | 35.0% | |
| TCCTCATCTCTTTCTACAAG+AGG | + | chr5_2:8731556-8731575 | Msa0754980:CDS | 40.0% | |
| ACCTCTTGTAGAAAGAGATG+AGG | - | chr5_2:8731560-8731579 | None:intergenic | 40.0% | |
| GTAGCAAAAGAACATGTACC+TGG | - | chr5_2:8731600-8731619 | None:intergenic | 40.0% | |
| !!! | ATGTCGTTGACTTTTTCAGC+TGG | - | chr5_2:8731830-8731849 | None:intergenic | 40.0% |
| AGAGGATGTAGTTCCTTTCT+TGG | - | chr5_2:8731864-8731883 | None:intergenic | 40.0% | |
| ! | TTCTAGCAAGAAATGATGGG+TGG | - | chr5_2:8731931-8731950 | None:intergenic | 40.0% |
| CCATAAATAACAGGTGTGGA+TGG | - | chr5_2:8731980-8731999 | None:intergenic | 40.0% | |
| ! | CCATCCACACCTGTTATTTA+TGG | + | chr5_2:8731977-8731996 | Msa0754980:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 8731515 | 8732027 | 8731515 | ID=Msa0754980;Name=Msa0754980 |
| chr5_2 | mRNA | 8731515 | 8732027 | 8731515 | ID=Msa0754980-mRNA-1;Parent=Msa0754980;Name=Msa0754980-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|1|1|2|0|125 |
| chr5_2 | exon | 8731515 | 8731600 | 8731515 | ID=Msa0754980-mRNA-1:exon:3964;Parent=Msa0754980-mRNA-1 |
| chr5_2 | exon | 8731736 | 8732027 | 8731736 | ID=Msa0754980-mRNA-1:exon:3965;Parent=Msa0754980-mRNA-1 |
| chr5_2 | CDS | 8731515 | 8731600 | 8731515 | ID=Msa0754980-mRNA-1:cds;Parent=Msa0754980-mRNA-1 |
| chr5_2 | CDS | 8731736 | 8732027 | 8731736 | ID=Msa0754980-mRNA-1:cds;Parent=Msa0754980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
