Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0756390 | sp|Q9SY66|FRS11_ARATH | 34.586 | 133 | 50 | 2 | 64 | 161 | 48 | 178 | 4.55e-18 | 83.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0756390 | A0A834L9S8 | 44.737 | 152 | 51 | 2 | 43 | 161 | 103 | 254 | 1.86e-29 | 119 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0756390 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0756390 | MtrunA17_Chr2g0276841 | 35.338 | 133 | 49 | 2 | 64 | 161 | 70 | 200 | 8.55e-20 | 85.1 |
| Msa0756390 | MtrunA17_Chr2g0290391 | 30.435 | 138 | 61 | 2 | 57 | 161 | 44 | 179 | 7.26e-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0756390 | AT1G10240.2 | 34.586 | 133 | 50 | 2 | 64 | 161 | 48 | 178 | 4.62e-19 | 83.6 |
| Msa0756390 | AT1G10240.1 | 34.586 | 133 | 50 | 2 | 64 | 161 | 48 | 178 | 4.62e-19 | 83.6 |
Find 22 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCACATGGTTCATTATT+TGG | 0.136012 | 5_2:-10641103 | None:intergenic |
| TCAATAACTTGCAATTCTTT+TGG | 0.220230 | 5_2:-10641470 | None:intergenic |
| AGAAAGCATTGCCTAATAAA+AGG | 0.305675 | 5_2:-10641196 | None:intergenic |
| CTCAGTGAGGAAGAAGCTTT+CGG | 0.314082 | 5_2:+10641217 | Msa0756390:CDS |
| GATTGAGAAACCACTCCTTT+TGG | 0.331753 | 5_2:-10641257 | None:intergenic |
| GCAAGGAAGTGCACTTGATC+TGG | 0.386234 | 5_2:-10641527 | None:intergenic |
| AAAGGAGTGGTTTCTCAATC+CGG | 0.480907 | 5_2:+10641260 | Msa0756390:CDS |
| TTTCTTCAATTTGATTGTCA+TGG | 0.488862 | 5_2:-10641432 | None:intergenic |
| TTTATGAGAATTATGCCAAA+AGG | 0.493635 | 5_2:+10641242 | Msa0756390:CDS |
| GAGAATTATGCCAAAAGGAG+TGG | 0.544730 | 5_2:+10641247 | Msa0756390:CDS |
| CTCAATCAATTGTTCTCATG+TGG | 0.560735 | 5_2:+10640772 | Msa0756390:CDS |
| TAGTAAATCATGATTGTGGT+TGG | 0.562568 | 5_2:-10641501 | None:intergenic |
| CATCTTCCTCAGAAATGTTG+CGG | 0.581639 | 5_2:-10641556 | None:intergenic |
| AAATGTTGCGGTAAGATGCA+AGG | 0.600031 | 5_2:-10641544 | None:intergenic |
| AATAGACAATCTGCAAGGTG+TGG | 0.611195 | 5_2:+10641398 | Msa0756390:CDS |
| AGGAAGTGCACTTGATCTGG+AGG | 0.619326 | 5_2:-10641524 | None:intergenic |
| ATCTTACCGCAACATTTCTG+AGG | 0.633790 | 5_2:+10641550 | Msa0756390:CDS |
| AACAAAATAGACAATCTGCA+AGG | 0.646541 | 5_2:+10641393 | Msa0756390:CDS |
| AGTGGTTTCTCAATCCGGAA+AGG | 0.654585 | 5_2:+10641265 | Msa0756390:CDS |
| TAGGCAATGCTTTCTCAGTG+AGG | 0.672173 | 5_2:+10641204 | Msa0756390:CDS |
| TGCAAGGTGTGGATGCAAAG+CGG | 0.693182 | 5_2:+10641409 | Msa0756390:CDS |
| GAGGTAGTAAATCATGATTG+TGG | 0.741310 | 5_2:-10641505 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTTCTTAAAAAAAAAAAT+GGG | + | chr5_2:10641290-10641309 | Msa0756390:intron | 10.0% |
| !! | GATTTCTTAAAAAAAAAAAT+GGG | + | chr5_2:10641290-10641309 | Msa0756390:intron | 10.0% |
| !!! | AATTCAATAGTAATATTTTG+TGG | + | chr5_2:10640978-10640997 | Msa0756390:intron | 15.0% |
| !!! | AATATTTTGTGGATAAATAA+TGG | + | chr5_2:10640989-10641008 | Msa0756390:intron | 15.0% |
| !! | TAATATGAATTTATTTGTGT+AGG | + | chr5_2:10641044-10641063 | Msa0756390:intron | 15.0% |
| !! | CGATTTCTTAAAAAAAAAAA+TGG | + | chr5_2:10641289-10641308 | Msa0756390:intron | 15.0% |
| !!! | AATTCAATAGTAATATTTTG+TGG | + | chr5_2:10640978-10640997 | Msa0756390:intron | 15.0% |
| !!! | AATATTTTGTGGATAAATAA+TGG | + | chr5_2:10640989-10641008 | Msa0756390:intron | 15.0% |
| !! | TAATATGAATTTATTTGTGT+AGG | + | chr5_2:10641044-10641063 | Msa0756390:intron | 15.0% |
| !! | CGATTTCTTAAAAAAAAAAA+TGG | + | chr5_2:10641289-10641308 | Msa0756390:intron | 15.0% |
| !! | AATTCGTATATAAGGAATTA+GGG | - | chr5_2:10640962-10640981 | None:intergenic | 20.0% |
| !! | TACTATTGAATTCGTATATA+AGG | - | chr5_2:10640970-10640989 | None:intergenic | 20.0% |
| !!! | ATTGTTTTGAACCTTTTATT+AGG | + | chr5_2:10641185-10641204 | Msa0756390:CDS | 20.0% |
| !!! | ATCATGATATTTTTTTTTCC+TGG | - | chr5_2:10641360-10641379 | None:intergenic | 20.0% |
| !! | AATTCGTATATAAGGAATTA+GGG | - | chr5_2:10640962-10640981 | None:intergenic | 20.0% |
| !! | TACTATTGAATTCGTATATA+AGG | - | chr5_2:10640970-10640989 | None:intergenic | 20.0% |
| !!! | ATTGTTTTGAACCTTTTATT+AGG | + | chr5_2:10641185-10641204 | Msa0756390:CDS | 20.0% |
| !!! | ATCATGATATTTTTTTTTCC+TGG | - | chr5_2:10641360-10641379 | None:intergenic | 20.0% |
| ! | ATTCGTATATAAGGAATTAG+GGG | - | chr5_2:10640961-10640980 | None:intergenic | 25.0% |
| ! | GAATTCGTATATAAGGAATT+AGG | - | chr5_2:10640963-10640982 | None:intergenic | 25.0% |
| ! | TGAATTTATTTGTGTAGGTT+AGG | + | chr5_2:10641049-10641068 | Msa0756390:intron | 25.0% |
| ! | TTATCACATGGTTCATTATT+TGG | - | chr5_2:10641106-10641125 | None:intergenic | 25.0% |
| ! | TTTATGAGAATTATGCCAAA+AGG | + | chr5_2:10641242-10641261 | Msa0756390:CDS | 25.0% |
| ! | AAAAATGGGATGATATACAT+AGG | + | chr5_2:10641304-10641323 | Msa0756390:intron | 25.0% |
| !!! | CTATTTTGTTGTTCTTTAGA+TGG | - | chr5_2:10641383-10641402 | None:intergenic | 25.0% |
| ! | TTTCTTCAATTTGATTGTCA+TGG | - | chr5_2:10641435-10641454 | None:intergenic | 25.0% |
| !!! | TTCTTTTGGAAAAATCTCAA+AGG | - | chr5_2:10641459-10641478 | None:intergenic | 25.0% |
| !!! | TCAATAACTTGCAATTCTTT+TGG | - | chr5_2:10641473-10641492 | None:intergenic | 25.0% |
| ! | ATTCGTATATAAGGAATTAG+GGG | - | chr5_2:10640961-10640980 | None:intergenic | 25.0% |
| ! | GAATTCGTATATAAGGAATT+AGG | - | chr5_2:10640963-10640982 | None:intergenic | 25.0% |
| ! | TGAATTTATTTGTGTAGGTT+AGG | + | chr5_2:10641049-10641068 | Msa0756390:intron | 25.0% |
| ! | TTATCACATGGTTCATTATT+TGG | - | chr5_2:10641106-10641125 | None:intergenic | 25.0% |
| ! | TTTATGAGAATTATGCCAAA+AGG | + | chr5_2:10641242-10641261 | Msa0756390:CDS | 25.0% |
| ! | AAAAATGGGATGATATACAT+AGG | + | chr5_2:10641304-10641323 | Msa0756390:intron | 25.0% |
| !!! | CTATTTTGTTGTTCTTTAGA+TGG | - | chr5_2:10641383-10641402 | None:intergenic | 25.0% |
| ! | TTTCTTCAATTTGATTGTCA+TGG | - | chr5_2:10641435-10641454 | None:intergenic | 25.0% |
| !!! | TTCTTTTGGAAAAATCTCAA+AGG | - | chr5_2:10641459-10641478 | None:intergenic | 25.0% |
| !!! | TCAATAACTTGCAATTCTTT+TGG | - | chr5_2:10641473-10641492 | None:intergenic | 25.0% |
| GTTGAGAACATATGAGAAAA+AGG | - | chr5_2:10640846-10640865 | None:intergenic | 30.0% | |
| GGTATATAATGTGTGACATA+AGG | - | chr5_2:10640874-10640893 | None:intergenic | 30.0% | |
| AAGGAAAGAAATTGAGAACA+AGG | - | chr5_2:10640900-10640919 | None:intergenic | 30.0% | |
| TAATATGTGTGTGTGACTTA+AGG | - | chr5_2:10640919-10640938 | None:intergenic | 30.0% | |
| ! | TCATTTTCTGAGTTATCACA+TGG | - | chr5_2:10641118-10641137 | None:intergenic | 30.0% |
| !! | AGAAAGCATTGCCTAATAAA+AGG | - | chr5_2:10641199-10641218 | None:intergenic | 30.0% |
| !! | TTTTAAGAAATCGACCTTTC+CGG | - | chr5_2:10641282-10641301 | None:intergenic | 30.0% |
| AACAAAATAGACAATCTGCA+AGG | + | chr5_2:10641393-10641412 | Msa0756390:CDS | 30.0% | |
| TAGTAAATCATGATTGTGGT+TGG | - | chr5_2:10641504-10641523 | None:intergenic | 30.0% | |
| GTTGAGAACATATGAGAAAA+AGG | - | chr5_2:10640846-10640865 | None:intergenic | 30.0% | |
| GGTATATAATGTGTGACATA+AGG | - | chr5_2:10640874-10640893 | None:intergenic | 30.0% | |
| AAGGAAAGAAATTGAGAACA+AGG | - | chr5_2:10640900-10640919 | None:intergenic | 30.0% | |
| TAATATGTGTGTGTGACTTA+AGG | - | chr5_2:10640919-10640938 | None:intergenic | 30.0% | |
| ! | TCATTTTCTGAGTTATCACA+TGG | - | chr5_2:10641118-10641137 | None:intergenic | 30.0% |
| !! | AGAAAGCATTGCCTAATAAA+AGG | - | chr5_2:10641199-10641218 | None:intergenic | 30.0% |
| !! | TTTTAAGAAATCGACCTTTC+CGG | - | chr5_2:10641282-10641301 | None:intergenic | 30.0% |
| AACAAAATAGACAATCTGCA+AGG | + | chr5_2:10641393-10641412 | Msa0756390:CDS | 30.0% | |
| TAGTAAATCATGATTGTGGT+TGG | - | chr5_2:10641504-10641523 | None:intergenic | 30.0% | |
| CTCAATCAATTGTTCTCATG+TGG | + | chr5_2:10640772-10640791 | Msa0756390:CDS | 35.0% | |
| AAGAAATTGAGAACAAGGTG+TGG | - | chr5_2:10640895-10640914 | None:intergenic | 35.0% | |
| AATGGGATGATATACATAGG+AGG | + | chr5_2:10641307-10641326 | Msa0756390:intron | 35.0% | |
| ATGGGATGATATACATAGGA+GGG | + | chr5_2:10641308-10641327 | Msa0756390:intron | 35.0% | |
| !!! | GATTTTTTCTGTCACTGAGA+AGG | + | chr5_2:10641330-10641349 | Msa0756390:intron | 35.0% |
| GAGGTAGTAAATCATGATTG+TGG | - | chr5_2:10641508-10641527 | None:intergenic | 35.0% | |
| CTCAATCAATTGTTCTCATG+TGG | + | chr5_2:10640772-10640791 | Msa0756390:CDS | 35.0% | |
| AAGAAATTGAGAACAAGGTG+TGG | - | chr5_2:10640895-10640914 | None:intergenic | 35.0% | |
| AATGGGATGATATACATAGG+AGG | + | chr5_2:10641307-10641326 | Msa0756390:intron | 35.0% | |
| ATGGGATGATATACATAGGA+GGG | + | chr5_2:10641308-10641327 | Msa0756390:intron | 35.0% | |
| !!! | GATTTTTTCTGTCACTGAGA+AGG | + | chr5_2:10641330-10641349 | Msa0756390:intron | 35.0% |
| GAGGTAGTAAATCATGATTG+TGG | - | chr5_2:10641508-10641527 | None:intergenic | 35.0% | |
| GAGAATTATGCCAAAAGGAG+TGG | + | chr5_2:10641247-10641266 | Msa0756390:CDS | 40.0% | |
| ! | GATTGAGAAACCACTCCTTT+TGG | - | chr5_2:10641260-10641279 | None:intergenic | 40.0% |
| AAAGGAGTGGTTTCTCAATC+CGG | + | chr5_2:10641260-10641279 | Msa0756390:CDS | 40.0% | |
| AATAGACAATCTGCAAGGTG+TGG | + | chr5_2:10641398-10641417 | Msa0756390:CDS | 40.0% | |
| AAATGTTGCGGTAAGATGCA+AGG | - | chr5_2:10641547-10641566 | None:intergenic | 40.0% | |
| ATCTTACCGCAACATTTCTG+AGG | + | chr5_2:10641550-10641569 | Msa0756390:CDS | 40.0% | |
| CATCTTCCTCAGAAATGTTG+CGG | - | chr5_2:10641559-10641578 | None:intergenic | 40.0% | |
| GAGAATTATGCCAAAAGGAG+TGG | + | chr5_2:10641247-10641266 | Msa0756390:CDS | 40.0% | |
| ! | GATTGAGAAACCACTCCTTT+TGG | - | chr5_2:10641260-10641279 | None:intergenic | 40.0% |
| AAAGGAGTGGTTTCTCAATC+CGG | + | chr5_2:10641260-10641279 | Msa0756390:CDS | 40.0% | |
| AATAGACAATCTGCAAGGTG+TGG | + | chr5_2:10641398-10641417 | Msa0756390:CDS | 40.0% | |
| AAATGTTGCGGTAAGATGCA+AGG | - | chr5_2:10641547-10641566 | None:intergenic | 40.0% | |
| ATCTTACCGCAACATTTCTG+AGG | + | chr5_2:10641550-10641569 | Msa0756390:CDS | 40.0% | |
| CATCTTCCTCAGAAATGTTG+CGG | - | chr5_2:10641559-10641578 | None:intergenic | 40.0% | |
| TAGGCAATGCTTTCTCAGTG+AGG | + | chr5_2:10641204-10641223 | Msa0756390:CDS | 45.0% | |
| CTCAGTGAGGAAGAAGCTTT+CGG | + | chr5_2:10641217-10641236 | Msa0756390:CDS | 45.0% | |
| AGTGGTTTCTCAATCCGGAA+AGG | + | chr5_2:10641265-10641284 | Msa0756390:CDS | 45.0% | |
| TGTCACTGAGAAGGATTTCC+AGG | + | chr5_2:10641339-10641358 | Msa0756390:intron | 45.0% | |
| TAGGCAATGCTTTCTCAGTG+AGG | + | chr5_2:10641204-10641223 | Msa0756390:CDS | 45.0% | |
| CTCAGTGAGGAAGAAGCTTT+CGG | + | chr5_2:10641217-10641236 | Msa0756390:CDS | 45.0% | |
| AGTGGTTTCTCAATCCGGAA+AGG | + | chr5_2:10641265-10641284 | Msa0756390:CDS | 45.0% | |
| TGTCACTGAGAAGGATTTCC+AGG | + | chr5_2:10641339-10641358 | Msa0756390:intron | 45.0% | |
| TGCAAGGTGTGGATGCAAAG+CGG | + | chr5_2:10641409-10641428 | Msa0756390:CDS | 50.0% | |
| ! | AGGAAGTGCACTTGATCTGG+AGG | - | chr5_2:10641527-10641546 | None:intergenic | 50.0% |
| ! | GCAAGGAAGTGCACTTGATC+TGG | - | chr5_2:10641530-10641549 | None:intergenic | 50.0% |
| TGCAAGGTGTGGATGCAAAG+CGG | + | chr5_2:10641409-10641428 | Msa0756390:CDS | 50.0% | |
| ! | AGGAAGTGCACTTGATCTGG+AGG | - | chr5_2:10641527-10641546 | None:intergenic | 50.0% |
| ! | GCAAGGAAGTGCACTTGATC+TGG | - | chr5_2:10641530-10641549 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 10640749 | 10641595 | 10640749 | ID=Msa0756390;Name=Msa0756390 |
| chr5_2 | mRNA | 10640749 | 10641595 | 10640749 | ID=Msa0756390-mRNA-1;Parent=Msa0756390;Name=Msa0756390-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|1|1|3|0|161 |
| chr5_2 | exon | 10640749 | 10640807 | 10640749 | ID=Msa0756390-mRNA-1:exon:4815;Parent=Msa0756390-mRNA-1 |
| chr5_2 | exon | 10641066 | 10641286 | 10641066 | ID=Msa0756390-mRNA-1:exon:4816;Parent=Msa0756390-mRNA-1 |
| chr5_2 | exon | 10641390 | 10641595 | 10641390 | ID=Msa0756390-mRNA-1:exon:4817;Parent=Msa0756390-mRNA-1 |
| chr5_2 | CDS | 10640749 | 10640807 | 10640749 | ID=Msa0756390-mRNA-1:cds;Parent=Msa0756390-mRNA-1 |
| chr5_2 | CDS | 10641066 | 10641286 | 10641066 | ID=Msa0756390-mRNA-1:cds;Parent=Msa0756390-mRNA-1 |
| chr5_2 | CDS | 10641390 | 10641595 | 10641390 | ID=Msa0756390-mRNA-1:cds;Parent=Msa0756390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
