AlfalfaGEDB | Msa0758110

M. sativa cultivar zhongmu-4 / Msa0758110

KEGG: -
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010648 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046982 GO:0046983 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF05142.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0758110	sp\|Q9SD40\|SRS1_ARATH	42.784	388	138	13	1	322	1	370	1.03e-73	234

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0758110	G7KGA2	86.269	335	21	8	1	322	1	323	0.0	521

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TFs/TRs:

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Gene ID	Type	Classification
Msa0758110	TF	SRS

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0758110	MtrunA17_Chr5g0404781	86.269	335	21	8	1	322	1	323	0.0	521
Msa0758110	MtrunA17_Chr8g0372461	54.624	346	118	12	1	322	1	331	3.10e-94	283
Msa0758110	MtrunA17_Chr3g0142171	42.331	326	141	14	1	317	1	288	2.18e-60	195
Msa0758110	MtrunA17_Chr1g0155791	52.609	230	84	7	107	322	120	338	9.49e-60	194
Msa0758110	MtrunA17_Chr5g0441921	50.314	159	55	4	109	249	124	276	4.15e-40	143
Msa0758110	MtrunA17_Chr4g0035591	46.575	146	58	1	106	251	17	142	4.91e-40	139
Msa0758110	MtrunA17_Chr3g0082511	36.889	225	113	5	57	254	69	291	1.11e-38	139
Msa0758110	MtrunA17_Chr8g0353111	44.048	168	74	3	109	257	124	290	4.78e-35	129
Msa0758110	MtrunA17_Chr2g0281741	58.140	43	18	0	101	143	142	184	2.01e-13	68.2
Msa0758110	MtrunA17_Chr0c01g0489181	59.459	37	15	0	107	143	65	101	6.23e-12	61.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0758110	AT3G51060.1	42.784	388	138	13	1	322	1	370	1.04e-74	234
Msa0758110	AT5G66350.1	47.154	369	110	19	1	322	1	331	1.89e-71	224
Msa0758110	AT5G66350.3	47.191	356	105	18	1	309	1	320	6.47e-69	218
Msa0758110	AT5G66350.2	47.191	356	105	18	1	309	1	320	1.01e-68	218
Msa0758110	AT1G75520.1	43.836	365	143	15	1	322	1	346	7.69e-66	211
Msa0758110	AT1G19790.2	40.872	367	148	14	1	321	1	344	2.04e-65	209
Msa0758110	AT1G19790.1	40.872	367	148	14	1	321	1	344	2.04e-65	209
Msa0758110	AT4G36260.1	37.537	341	170	14	1	320	1	319	9.59e-47	160
Msa0758110	AT2G18120.1	41.860	258	86	11	1	251	6	206	2.93e-45	154
Msa0758110	AT2G21400.2	52.740	146	54	3	106	249	7	139	2.62e-44	150

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Find 93 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAGTGTTGTTGATGT+TGG	0.482819	5_2:+13209265	None:intergenic
AAACTCATGTTAAGAGTACT+TGG	0.425175	5_2:-13208993	Msa0758110:CDS
AACTCATGTTAAGAGTACTT+GGG	0.622414	5_2:-13208992	Msa0758110:CDS
AAGAATTGCGTACCACTAGA+AGG	0.471301	5_2:+13207852	None:intergenic
AAGGTTTGGTTAGTTGGTTG+AGG	0.513889	5_2:+13209655	None:intergenic
AATAGATCTACTGTGTTTGT+AGG	0.376062	5_2:-13209135	Msa0758110:CDS
AATAGTTACAATATGGCTAC+CGG	0.553111	5_2:-13208017	Msa0758110:CDS
AATTCCTTCATGCCTTCTAG+TGG	0.576741	5_2:-13207864	Msa0758110:CDS
AATTTGTGGTTAAATTTGAT+TGG	0.429606	5_2:-13209527	Msa0758110:five_prime_UTR
ACAGAAGAGATAAGGTTTAA+GGG	0.430955	5_2:+13207948	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAATCCAACAACAGAATTC+TGG	-	chr5_2:13208287-13208306	Msa0758110:intron	30.0%
TTATACCAGAATTCTGTTGT+TGG	+	chr5_2:13208295-13208314	None:intergenic	30.0%
AATAGATCTACTGTGTTTGT+AGG	-	chr5_2:13208499-13208518	Msa0758110:intron	30.0%
GTATAAGTTGTCAAGATTGT+GGG	-	chr5_2:13208548-13208567	Msa0758110:intron	30.0%
ATTCTCTTCCAAAACTTTCT+CGG	+	chr5_2:13208951-13208970	None:intergenic	30.0%
AATGAATGGGATCAATAACA+GGG	-	chr5_2:13209039-13209058	Msa0758110:CDS	30.0%
TATTCAACAGCTGTAAACAT+TGG	-	chr5_2:13209542-13209561	Msa0758110:five_prime_UTR	30.0%
TAACTATTATTCCCATGATC+TGG	+	chr5_2:13209605-13209624	None:intergenic	30.0%
AATAGTTACAATATGGCTAC+CGG	-	chr5_2:13209617-13209636	Msa0758110:five_prime_UTR	30.0%
ACAGAAGAGATAAGGTTTAA+GGG	+	chr5_2:13209689-13209708	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	13207745	13209911	13207745	ID=Msa0758110;Name=Msa0758110
chr5_2	mRNA	13207745	13209911	13207745	ID=Msa0758110-mRNA-1;Parent=Msa0758110;Name=Msa0758110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=487\|1\|1\|1\|0\|0.5\|2\|88\|322
chr5_2	exon	13207745	13208215	13207745	ID=Msa0758110-mRNA-1:exon:5945;Parent=Msa0758110-mRNA-1
chr5_2	exon	13208839	13209911	13208839	ID=Msa0758110-mRNA-1:exon:5944;Parent=Msa0758110-mRNA-1
chr5_2	five_prime_UTR	13209425	13209911	13209425	ID=Msa0758110-mRNA-1:five_prime_utr;Parent=Msa0758110-mRNA-1
chr5_2	CDS	13208839	13209424	13208839	ID=Msa0758110-mRNA-1:cds;Parent=Msa0758110-mRNA-1
chr5_2	CDS	13207833	13208215	13207833	ID=Msa0758110-mRNA-1:cds;Parent=Msa0758110-mRNA-1
chr5_2	three_prime_UTR	13207745	13207832	13207745	ID=Msa0758110-mRNA-1:three_prime_utr;Parent=Msa0758110-mRNA-1

Gene Sequence

>Msa0758110

ATGGCTGGTTTATTTTCATTAGGAAGAACTGAAGAAGAAGAAAACCAACAGAATAATAATCCAACAACAGAATTCTGGTATAATAAGAATGATGATTCTGTTGTTTCTAATTCATACCGTGGTTTTGAGATTTGGAACCAACATCAACAACACTTGTTTTCTTTACAACAACCTCAGCAGCAGCAGCAGCAGCAGCAGCAACAAGATCTTTACTCCTCTGGTGTTGTGTTAGGAGTGGGACCTACTAGGGTTTGTTCTTCTGATGAGAATAGATCTACTGTGTTTGTAGGAAGTGGAAGTGGAAGTGGAAGTGGAGGTATAAGTTGTCAAGATTGTGGGAATCAAGCTAAGAAAGATTGTCCTCATATGAGATGTAGAACTTGTTGTAAGAGTAGAGGTTTTGATTGTCAAACTCATGTTAAGAGTACTTGGGTTCCTGCTGCTAGAAGAAGAGAAAGACAACAACAACAACTTTCTGCTCCTAAATCATCTCAACAACATGGAGAGAGAGATCTTAACAATAACAATTCCTCTTTAGCTTGCACTCGTTTACCTACAAACCCTTCACTCACTCTCCCTTCAGCAGGGTTATTGGATGAGATGAATTTTCCAGCAGTGGTGAATTCTCCAGCAGAGTTCAGGTGCGTGAGAGTTAGCTCAATTGATGAGAACGAAGAGGAGTATGCATATTCAACAGCTGTAAACATTGGAGGGCATGTTTTTAGAGGAATTTTGTATGATTATGGTCCAGATCATGGGAATAATAGTTACAATATGGCTACCGGAAACGCCTCTAATTCTAGTGGTACACTCCTTGAAGGGGTGGCTCAGCCCTTAAACCTTATCTCTTCTGTCCCTGATGCTACTGCTGTTGGGGAAATTGTTGATCCTTCTTCTTTGTATCCAGCTCCACTTAATTCCTTCATGCCTTCTAGTGGTACGCAATTCTTCCCCCACCCAAGATCTTGA

Protein sequence

>Msa0758110

MAGLFSLGRTEEEENQQNNNPTTEFWYNKNDDSVVSNSYRGFEIWNQHQQHLFSLQQPQQQQQQQQQQDLYSSGVVLGVGPTRVCSSDENRSTVFVGSGSGSGSGGISCQDCGNQAKKDCPHMRCRTCCKSRGFDCQTHVKSTWVPAARRRERQQQQLSAPKSSQQHGERDLNNNNSSLACTRLPTNPSLTLPSAGLLDEMNFPAVVNSPAEFRCVRVSSIDENEEEYAYSTAVNIGGHVFRGILYDYGPDHGNNSYNMATGNASNSSGTLLEGVAQPLNLISSVPDATAVGEIVDPSSLYPAPLNSFMPSSGTQFFPHPRS*