AlfalfaGEDB | Msa0760680

M. sativa cultivar zhongmu-4 / Msa0760680

KEGG: -
GO: -
PFAM: PF08880.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760680	sp\|Q6AWY4\|GRF5_ORYSJ	35.758	330	157	9	64	365	25	327	2.03e-43	157

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760680	G7K6W0	95.482	332	7	2	48	379	1	324	0.0	650

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TFs/TRs:

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Gene ID	Type	Classification
Msa0760680	TF	GRF

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760680	MtrunA17_Chr5g0409471	95.482	332	7	2	48	379	1	324	0.0	650
Msa0760680	MtrunA17_Chr4g0021261	47.418	213	77	9	63	262	25	215	7.00e-49	168
Msa0760680	MtrunA17_Chr4g0070591	34.058	276	146	7	63	307	9	279	4.99e-42	150
Msa0760680	MtrunA17_Chr2g0300511	60.177	113	29	2	63	159	7	119	7.53e-40	144
Msa0760680	MtrunA17_Chr8g0343881	58.333	108	33	2	64	159	12	119	1.65e-38	141
Msa0760680	MtrunA17_Chr1g0152191	56.897	116	34	2	63	162	144	259	2.54e-36	139
Msa0760680	MtrunA17_Chr7g0265931	62.037	108	29	4	64	159	79	186	2.08e-35	133
Msa0760680	MtrunA17_Chr3g0127641	53.000	100	39	2	61	159	110	202	9.19e-27	111
Msa0760680	MtrunA17_Chr8g0351521	47.619	105	43	2	62	161	53	150	1.01e-19	90.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760680	AT3G13960.1	57.273	110	35	2	62	159	15	124	6.00e-38	141
Msa0760680	AT4G37740.1	53.600	125	48	2	63	177	164	288	3.43e-37	141
Msa0760680	AT3G52910.1	56.250	112	33	3	64	159	83	194	3.97e-33	127
Msa0760680	AT2G36400.1	56.250	112	34	3	64	160	77	188	6.22e-33	127
Msa0760680	AT2G22840.1	57.944	107	35	2	63	159	133	239	2.65e-30	122
Msa0760680	AT2G06200.2	50.400	125	39	3	56	157	38	162	4.36e-30	117
Msa0760680	AT2G06200.1	52.066	121	35	3	60	157	3	123	7.50e-30	115
Msa0760680	AT4G24150.3	46.610	118	47	2	64	165	112	229	2.35e-23	101
Msa0760680	AT4G24150.2	46.610	118	47	2	64	165	150	267	5.76e-23	100
Msa0760680	AT5G53660.1	49.485	97	44	1	63	159	59	150	3.52e-22	97.1

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Find 98 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTGACCCAGAACCAGGT+AGG	0.671324	5_2:-17284741	Msa0760680:CDS
AACCTCTTACAGAAGGATCT+AGG	0.500686	5_2:-17282571	Msa0760680:CDS
AAGAAGCATAGGGTCCATGT+GGG	0.631241	5_2:+17284393	None:intergenic
AAGGTTTAAGTTTCAATCTT+GGG	0.373864	5_2:-17284426	Msa0760680:CDS
AATGCAACAAATCAAGAAAA+AGG	0.318694	5_2:+17282036	None:intergenic
AATGGAGTATAGGCCAAGCA+AGG	0.658290	5_2:-17282260	Msa0760680:CDS
ACACTTCCTTTGCATACAAA+TGG	0.453684	5_2:-17284457	Msa0760680:CDS
ACAGTAGGAAAATTGAAGCT+TGG	0.552947	5_2:+17282127	None:intergenic
ACTGTGAACGGTGGATGAGT+TGG	0.621139	5_2:+17284996	None:intergenic
ACTTCACAGCAACTTGGTGC+TGG	0.533655	5_2:-17284541	Msa0760680:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAGGAAGATTAACGTTGTT+GGG	+	chr5_2:17282287-17282306	None:intergenic	30.0%
ATAAGGAAGATTAACGTTGT+TGG	+	chr5_2:17282288-17282307	None:intergenic	30.0%
TAACTCTTCACAAGAAATCA+TGG	+	chr5_2:17283042-17283061	None:intergenic	30.0%
AAGTTACATAACATGTGATG+TGG	-	chr5_2:17283143-17283162	Msa0760680:intron	30.0%
CACATTATTTATTCGATACC+TGG	-	chr5_2:17283451-17283470	Msa0760680:intron	30.0%
TAGTTCACTCACATCTAAAA+AGG	+	chr5_2:17283487-17283506	None:intergenic	30.0%
ATTGGAAATCAAGGGAATTA+TGG	-	chr5_2:17283527-17283546	Msa0760680:intron	30.0%
CGATAGAGTTAAAGCAATTA+AGG	-	chr5_2:17283597-17283616	Msa0760680:intron	30.0%
ATTATGGGATAACATGTTGA+AGG	-	chr5_2:17283804-17283823	Msa0760680:intron	30.0%
GATAACATGTTGAAGGAATT+TGG	-	chr5_2:17283811-17283830	Msa0760680:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	17281899	17285248	17281899	ID=Msa0760680;Name=Msa0760680
chr5_2	mRNA	17281899	17285248	17281899	ID=Msa0760680-mRNA-1;Parent=Msa0760680;Name=Msa0760680-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0.66\|0.5\|1\|1\|1\|4\|326\|379
chr5_2	exon	17281899	17282624	17281899	ID=Msa0760680-mRNA-1:exon:7661;Parent=Msa0760680-mRNA-1
chr5_2	exon	17284364	17284781	17284364	ID=Msa0760680-mRNA-1:exon:7660;Parent=Msa0760680-mRNA-1
chr5_2	exon	17284871	17285121	17284871	ID=Msa0760680-mRNA-1:exon:7659;Parent=Msa0760680-mRNA-1
chr5_2	exon	17285178	17285248	17285178	ID=Msa0760680-mRNA-1:exon:7658;Parent=Msa0760680-mRNA-1
chr5_2	CDS	17285178	17285248	17285178	ID=Msa0760680-mRNA-1:cds;Parent=Msa0760680-mRNA-1
chr5_2	CDS	17284871	17285121	17284871	ID=Msa0760680-mRNA-1:cds;Parent=Msa0760680-mRNA-1
chr5_2	CDS	17284364	17284781	17284364	ID=Msa0760680-mRNA-1:cds;Parent=Msa0760680-mRNA-1
chr5_2	CDS	17282225	17282624	17282225	ID=Msa0760680-mRNA-1:cds;Parent=Msa0760680-mRNA-1
chr5_2	three_prime_UTR	17281899	17282224	17281899	ID=Msa0760680-mRNA-1:three_prime_utr;Parent=Msa0760680-mRNA-1

Gene Sequence

>Msa0760680

ATGGCTCAACTGGTTTCCTCTAAGGTAAGCTCCCCACCCTTTCCTCCTCCCTCACCACCACCACAACCACCATTCAATCCTTCTTCATCATCATCATCATCTTCACAACATCATCACCTTAAAAACCCCAAAAACAAAAACATGAGCATTCAACAACCGCCGTTGATGTGGTCACCAACTCATCCACCGTTCACAGTCTCCCAATGGCAAGAGCTAGAACATCAAGCTCTCATCTTCAAGTATCTGAAAGCTGGTTTATCTGTTCCTCCTGACCTCCTTTTACCTATTCGGAAAAGTCTTCAATTCATGTCTCATCCATCTCTGGGTTATTATGGAAAAAAAATTGACCCAGAACCAGGTAGGTGTAGAAGAACAGATGGCAAAAAATGGAGATGTGCTAGGGATGCACATCCTGATTCTAAGTACTGTGACCGTCATATGATTCGACGTCGATACCGTTCAAGAAAGCCTGTGGAATCTTCTTCTTCTTCTTCTTGTTCACAATCTTCATCTTCAGTTTCAGTTTCTACTACTAGTACTACTTCACAGCAACTTGGTGCTGGTGGTTCTAGTGGAAGTACTAGTACTGTTTCTGCTTCTGTTACTGCTACTTCAACTTTTCATACACTTCCTTTGCATACAAATGGTACTCGTGAAGGTTTAAGTTTCAATCTTGGGAACACCATGCCCCACATGGACCCTATGCTTCTTCCTCTTCAGGGTTCTAAGAAACCTTATAGGTTTGGACTGAACTCGGAAGCAGAGGAGAACAACCTCTTACAGAAGGATCTAGGAACTGTGAAGTATCAAGGTTATGACTTCACTTCAGATGGGATGTGGTATAATAACATGTCTCAGATTTCATCAAACACTGTTTCAGAATCAAGGAATTGTTCTTCCATGGTTGACAACTACAACTTCCAACAGCGAACAACAAGAGAACCTGAGCTGTTTAACCTCGACGCTGCAAGATCCAAGGAAATTGTCTTTAACGGCCAGCTAGGTAGTTTGAAACAGGAATATCCATCCTCTCAGTCTCTCTTCAGTGACTGGCAGTGGAAGAAAGATTTAGCCTCCTCTGCAATGGAGTATAGGCCAAGCAAGGATTTTAACACCAATCCAGATGTCAATGTTGATTGA

Protein sequence

>Msa0760680

MAQLVSSKVSSPPFPPPSPPPQPPFNPSSSSSSSSQHHHLKNPKNKNMSIQQPPLMWSPTHPPFTVSQWQELEHQALIFKYLKAGLSVPPDLLLPIRKSLQFMSHPSLGYYGKKIDPEPGRCRRTDGKKWRCARDAHPDSKYCDRHMIRRRYRSRKPVESSSSSSCSQSSSSVSVSTTSTTSQQLGAGGSSGSTSTVSASVTATSTFHTLPLHTNGTREGLSFNLGNTMPHMDPMLLPLQGSKKPYRFGLNSEAEENNLLQKDLGTVKYQGYDFTSDGMWYNNMSQISSNTVSESRNCSSMVDNYNFQQRTTREPELFNLDAARSKEIVFNGQLGSLKQEYPSSQSLFSDWQWKKDLASSAMEYRPSKDFNTNPDVNVD*