AlfalfaGEDB | Msa0763360

M. sativa cultivar zhongmu-4 / Msa0763360

KEGG: K17498
GO: GO:0000414 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010793 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032386 GO:0032784 GO:0032870 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0033157 GO:0033993 GO:0035065 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043401 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046822 GO:0046825 GO:0046831 GO:0048545 GO:0050684 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051171 GO:0051223 GO:0051246 GO:0051252 GO:0051716 GO:0060255 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0080090 GO:0090087 GO:0090239 GO:1901700 GO:1901701 GO:1901983 GO:1902275 GO:1903311 GO:1903506 GO:1903827 GO:2000112 GO:2000197 GO:2000756 GO:2001141 GO:2001253
PFAM: PF08711.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0763360	sp\|F4ICK8\|IWS1_ARATH	63.048	525	154	11	1	508	1	502	0.0	581

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0763360	G7K9K8	96.071	509	6	2	1	508	1	496	0.0	970

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TFs/TRs:

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Gene ID	Type	Classification
Msa0763360	TR	IWS1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0763360	MtrunA17_Chr5g0410141	96.071	509	6	2	1	508	1	496	0.0	970

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0763360	AT1G32130.1	63.048	525	154	11	1	508	1	502	0.0	581
Msa0763360	AT1G32130.2	62.095	525	150	12	1	508	1	493	0.0	563
Msa0763360	AT4G19000.1	51.839	299	129	5	215	508	125	413	2.76e-88	277

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Find 168 sgRNAs with CRISPR-Local

Find 350 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAAGGAAGGTGGGTC+TGG	0.285737	5_2:+22530433	Msa0763360:CDS
AAAAGTGGGAATGATCGTGA+AGG	0.734592	5_2:+22530541	Msa0763360:CDS
AAACTGAGTGTCGAAGGTCG+TGG	0.635870	5_2:+22534367	Msa0763360:CDS
AAAGGAAGGAAGGTGGGTCT+GGG	0.392644	5_2:+22530434	Msa0763360:CDS
AAAGTAAAGCCACAGTACAT+TGG	0.528148	5_2:+22534632	Msa0763360:three_prime_UTR
AAATCCAAAGGTGTGGCTTC+TGG	0.295709	5_2:-22533161	None:intergenic
AAATTTGGTGGTTCTAAGAG+AGG	0.579251	5_2:+22530508	Msa0763360:CDS
AAGAAGGAGAAGAGGCTGAA+AGG	0.431465	5_2:+22530457	Msa0763360:CDS
AAGAGACGGTGATCTTGATT+TGG	0.370436	5_2:+22532999	Msa0763360:CDS
AAGAGGATGCTGAGCTTAAT+CGG	0.405962	5_2:+22531860	Msa0763360:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAATTGTGTTGTGAATTG+GGG	-	chr5_2:22530013-22530032	None:intergenic	30.0%
TCTCTATGATGTGTACTTTA+TGG	+	chr5_2:22531176-22531195	Msa0763360:intron	30.0%
ACTAATAAAGCAATTTCTGC+AGG	-	chr5_2:22531805-22531824	None:intergenic	30.0%
ATGTGAACAAAACAACGATA+GGG	+	chr5_2:22532334-22532353	Msa0763360:intron	30.0%
GGAATTTGGAAGTAATATAC+TGG	+	chr5_2:22532380-22532399	Msa0763360:intron	30.0%
AAATGCTAGAGAAGAAAACT+CGG	+	chr5_2:22532600-22532619	Msa0763360:intron	30.0%
CGAGTACTCTTGTTAAATAT+AGG	-	chr5_2:22532789-22532808	None:intergenic	30.0%
ATGATTTAAACATGGGATAC+TGG	+	chr5_2:22533618-22533637	Msa0763360:intron	30.0%
AAACATGATATCCACAAGTT+GGG	-	chr5_2:22533793-22533812	None:intergenic	30.0%
AAAACATGATATCCACAAGT+TGG	-	chr5_2:22533794-22533813	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	22529932	22534801	22529932	ID=Msa0763360;Name=Msa0763360
chr5_2	mRNA	22529932	22534801	22529932	ID=Msa0763360-mRNA-1;Parent=Msa0763360;Name=Msa0763360-mRNA-1;_AED=0.11;_eAED=0.11;_QI=109\|0.9\|0.90\|1\|1\|1\|11\|393\|508
chr5_2	exon	22529932	22530060	22529932	ID=Msa0763360-mRNA-1:exon:9227;Parent=Msa0763360-mRNA-1
chr5_2	exon	22530150	22530606	22530150	ID=Msa0763360-mRNA-1:exon:9228;Parent=Msa0763360-mRNA-1
chr5_2	exon	22531307	22531459	22531307	ID=Msa0763360-mRNA-1:exon:9229;Parent=Msa0763360-mRNA-1
chr5_2	exon	22531724	22531944	22531724	ID=Msa0763360-mRNA-1:exon:9230;Parent=Msa0763360-mRNA-1
chr5_2	exon	22532035	22532164	22532035	ID=Msa0763360-mRNA-1:exon:9231;Parent=Msa0763360-mRNA-1
chr5_2	exon	22532254	22532316	22532254	ID=Msa0763360-mRNA-1:exon:9232;Parent=Msa0763360-mRNA-1
chr5_2	exon	22532428	22532505	22532428	ID=Msa0763360-mRNA-1:exon:9233;Parent=Msa0763360-mRNA-1
chr5_2	exon	22532780	22532871	22532780	ID=Msa0763360-mRNA-1:exon:9234;Parent=Msa0763360-mRNA-1
chr5_2	exon	22532969	22533031	22532969	ID=Msa0763360-mRNA-1:exon:9235;Parent=Msa0763360-mRNA-1
chr5_2	exon	22533118	22533268	22533118	ID=Msa0763360-mRNA-1:exon:9236;Parent=Msa0763360-mRNA-1
chr5_2	exon	22534310	22534801	22534310	ID=Msa0763360-mRNA-1:exon:9237;Parent=Msa0763360-mRNA-1
chr5_2	five_prime_UTR	22529932	22530040	22529932	ID=Msa0763360-mRNA-1:five_prime_utr;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22530041	22530060	22530041	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22530150	22530606	22530150	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22531307	22531459	22531307	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22531724	22531944	22531724	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22532035	22532164	22532035	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22532254	22532316	22532254	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22532428	22532505	22532428	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22532780	22532871	22532780	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22532969	22533031	22532969	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22533118	22533268	22533118	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	CDS	22534310	22534408	22534310	ID=Msa0763360-mRNA-1:cds;Parent=Msa0763360-mRNA-1
chr5_2	three_prime_UTR	22534409	22534801	22534409	ID=Msa0763360-mRNA-1:three_prime_utr;Parent=Msa0763360-mRNA-1

Gene Sequence

>Msa0763360

ATGGGTTACGAAGACAATCCGTATCGTGATGAAGACGGTGAACCGTTGATGGATTACGACGACGTACAATCCGACGGCGAAGGATCACCGGAGCCACAACAACTCGACGATTTCGAAGAAGAAGATGTCGACAATTTTCATGATCGTGCCCGGTCACAGACACCGGTTTACGACAACGATTCGTCGAAATCAAAGCCGAGGAAACGCTTGATTAAGAAAAGTGATACCGGAAAACAGTCAATGGCGCTGCCACCGGAGCTGGAAGATGAATTGGAGGAGGAAGATGAAGGGAGGAAGCGGAAGAAAAGGAAGGAAGGTGGGTCTGGGAAGAAGGAGAAGAGGCTGAAAGGGGGTAGTAGTAGTGGAAAGGGTGGGTCTAAATTTGGTGGTTCTAAGAGAGGGGTTGGTGGGAAAAGTGGGAATGATCGTGAAGGTGAAGTTAATGAGATGTGGGATGCACTTGCTGGAAATTCTGAGTATCTGATGTTAGTTTACTTTGTTGTTTCTGTCATCAAGGATGATAATGAAGGTGCCAGGAATATGGATGATGATAACTTTATAGACGACACTGGAGTAGAACCTGCTTTGTATGGTTACGATGAACCGCGTTCTCCTGGCGATGCTCCACAGGCCGAGGAAGGTGAGGAAGATGATGAAATCAAGGATCTTTTCAAGATGGGGAAGAAAAAGAAGAAGAACGAGAGAAGTCCTGCAGAAATTGCTTTATTAGTTGAGAATGTTATGGCTGAGCTTGAGGTTACAGCAGAAGAGGATGCTGAGCTTAATCGGCAGCACAAGCCCGCAGTCAATAAACTCAAGAAGTTGCCTCTTCTTATAGAAGTCCTCTCAAAGAAACAGCTTCAGCTAGAGTTTTTAGATCACGGAGTGCTAAATTTATTGAAGAGTTGGCTTGAGCCACTTCCTGATGGAAGTCTGCCAAATATCAACATACGGACAGCAATTTTGAAGATTTTGAATGATTTACCTATAGATCTAGAGCATTATGATAGAAGAGAACAGCTGAAGAGAAGTGGTCTTGGAAAGGTTATTATGTTTTTATCAAGGTCCGATGAAGAAATCAATGTAAATAGGAGATTAGCCAAGGATTTGGTTGATAAATGGAGCCGACCTATATTTAACAAGAGTACTCGGTTTGAAGACATGCGAAATACTGAGGATGATAGGGTTCCTTACAGGAGGCCATCGGTTAAAAAGCCAGCAACTAAAGCTGCAGGGATGCAATCAAGAGACGGTGATCTTGATTTGGATCTTTCACAGCCTAGGTCCGGAGAGTCGTCTTCCAGGCAACATGCATCAAGGCCAGAAGCCACACCTTTGGATTTTGTGATCCGTCCCCAATCTAAAATTGACCCCGATGAAATCAGAGCACGAGCAAAACAAGCTACACAAGACCAGCATCGTATGAAGATGAATAAGAAATTGCAGCAGTTGAGGGCACCAAAAAAGAAGCAGCTTCAGGCTACAAAACTGAGTGTCGAAGGTCGTGGTATGGCCAAATACTTTTAA

Protein sequence

>Msa0763360

MGYEDNPYRDEDGEPLMDYDDVQSDGEGSPEPQQLDDFEEEDVDNFHDRARSQTPVYDNDSSKSKPRKRLIKKSDTGKQSMALPPELEDELEEEDEGRKRKKRKEGGSGKKEKRLKGGSSSGKGGSKFGGSKRGVGGKSGNDREGEVNEMWDALAGNSEYLMLVYFVVSVIKDDNEGARNMDDDNFIDDTGVEPALYGYDEPRSPGDAPQAEEGEEDDEIKDLFKMGKKKKKNERSPAEIALLVENVMAELEVTAEEDAELNRQHKPAVNKLKKLPLLIEVLSKKQLQLEFLDHGVLNLLKSWLEPLPDGSLPNINIRTAILKILNDLPIDLEHYDRREQLKRSGLGKVIMFLSRSDEEINVNRRLAKDLVDKWSRPIFNKSTRFEDMRNTEDDRVPYRRPSVKKPATKAAGMQSRDGDLDLDLSQPRSGESSSRQHASRPEATPLDFVIRPQSKIDPDEIRARAKQATQDQHRMKMNKKLQQLRAPKKKQLQATKLSVEGRGMAKYF*