AlfalfaGEDB | Msa0766060

M. sativa cultivar zhongmu-4 / Msa0766060

KEGG: -
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766060	AES71801.2	86.134	238	13	3	1	225	99	329	1.63e-137	406

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766060	sp\|Q8VYT3\|Y4052_ARATH	74.803	127	15	2	3	112	392	518	5.94e-53	186

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766060	G7J8X0	86.134	238	13	3	1	225	99	329	7.76e-138	406

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766060	MtrunA17_Chr4g0073471	83.594	128	4	2	2	112	383	510	4.00e-62	207
Msa0766060	MtrunA17_Chr7g0220741	72.656	128	18	2	2	112	394	521	3.84e-52	181
Msa0766060	MtrunA17_Chr0c03g0490031	72.656	128	18	2	2	112	389	516	4.94e-52	180
Msa0766060	MtrunA17_Chr6g0456231	71.875	128	19	2	2	112	392	519	1.18e-50	176
Msa0766060	MtrunA17_Chr5g0413291	66.406	128	26	2	2	112	394	521	2.27e-49	173
Msa0766060	MtrunA17_Chr2g0284101	58.000	150	40	3	2	134	390	533	5.82e-45	161
Msa0766060	MtrunA17_Chr1g0200911	56.349	126	37	3	5	112	399	524	4.36e-37	139
Msa0766060	MtrunA17_Chr2g0278831	53.543	127	41	3	4	112	387	513	7.92e-36	135
Msa0766060	MtrunA17_Chr8g0382101	52.800	125	42	2	2	109	375	499	1.41e-33	129
Msa0766060	MtrunA17_Chr2g0278861	50.781	128	45	3	3	112	382	509	4.12e-32	125

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766060	AT2G23950.2	73.438	128	17	2	3	113	388	515	5.44e-55	187
Msa0766060	AT4G30520.1	74.803	127	15	2	3	112	392	518	6.03e-54	186
Msa0766060	AT2G23950.1	73.228	127	17	2	3	112	388	514	2.56e-53	184
Msa0766060	AT3G25560.1	70.079	127	21	2	3	112	401	527	1.81e-50	177
Msa0766060	AT3G25560.2	70.079	127	21	2	3	112	402	528	1.83e-50	177
Msa0766060	AT3G25560.3	70.079	127	21	2	3	112	401	527	2.34e-50	176
Msa0766060	AT1G60800.2	67.188	128	25	2	2	112	326	453	7.52e-50	174
Msa0766060	AT1G60800.1	67.188	128	25	2	2	112	393	520	2.87e-49	173
Msa0766060	AT5G16000.1	70.079	127	21	2	3	112	401	527	3.52e-49	173
Msa0766060	AT5G45780.2	58.088	136	40	2	3	121	349	484	1.89e-41	151

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Find 69 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTGCTAATGTGCTTC+TGG	0.309989	5_2:-27471045	Msa0766060:CDS
AAAAGGGTGCTATGCTAGAG+TGG	0.561565	5_2:-27470797	Msa0766060:CDS
AAAGCCACCACAAAGTAGAG+AGG	0.708014	5_2:+27469192	None:intergenic
AAAGGGTGCTATGCTAGAGT+GGG	0.619734	5_2:-27470796	Msa0766060:CDS
AACAATGCCACGTACAGCAG+TGG	0.627238	5_2:+27470950	None:intergenic
AAGAAATGTCTTCAAATTAT+TGG	0.217483	5_2:-27470106	Msa0766060:CDS
AAGAAGAAGGTTTGAAAGGA+TGG	0.493952	5_2:+27469225	None:intergenic
AATACTTGACAACATCAATT+TGG	0.252020	5_2:+27469260	None:intergenic
AATGTTTGGATTGAAGAGTA+TGG	0.409462	5_2:+27470768	None:intergenic
ACAAGAAAGAGGATAGCAAT+TGG	0.467119	5_2:-27471131	Msa0766060:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAACACTTCGTTAACATTGA+AGG	-	chr5_2:27469199-27469218	Msa0766060:CDS	30.0%
AATATGCGATGTGATTTGAA+TGG	-	chr5_2:27469299-27469318	Msa0766060:CDS	30.0%
ATATTCATTGTCTTGCTCAT+CGG	-	chr5_2:27469363-27469382	Msa0766060:CDS	30.0%
AGCAACAATCGTAACAAATT+TGG	-	chr5_2:27469514-27469533	Msa0766060:intron	30.0%
GTAACAAATTTGGTTGACAA+TGG	-	chr5_2:27469524-27469543	Msa0766060:intron	30.0%
GGTGATATTGAAATAGATAG+TGG	-	chr5_2:27469545-27469564	Msa0766060:intron	30.0%
TCAAAGCTTATTATGCATGT+AGG	-	chr5_2:27469634-27469653	Msa0766060:intron	30.0%
CAATGATGCTAATCATGATT+TGG	-	chr5_2:27469666-27469685	Msa0766060:intron	30.0%
GCATTGCATTCAATATATCT+GGG	+	chr5_2:27469789-27469808	None:intergenic	30.0%
TATATTGAATGCAATGCCTT+TGG	-	chr5_2:27469792-27469811	Msa0766060:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	27469156	27472584	27469156	ID=Msa0766060;Name=Msa0766060
chr5_2	mRNA	27469156	27472584	27469156	ID=Msa0766060-mRNA-1;Parent=Msa0766060;Name=Msa0766060-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0\|0\|0.85\|0.16\|0.28\|7\|0\|306
chr5_2	exon	27472581	27472584	27472581	ID=Msa0766060-mRNA-1:exon:10756;Parent=Msa0766060-mRNA-1
chr5_2	exon	27471101	27471176	27471101	ID=Msa0766060-mRNA-1:exon:10755;Parent=Msa0766060-mRNA-1
chr5_2	exon	27471025	27471073	27471025	ID=Msa0766060-mRNA-1:exon:10754;Parent=Msa0766060-mRNA-1
chr5_2	exon	27470765	27471000	27470765	ID=Msa0766060-mRNA-1:exon:10753;Parent=Msa0766060-mRNA-1
chr5_2	exon	27470069	27470189	27470069	ID=Msa0766060-mRNA-1:exon:10752;Parent=Msa0766060-mRNA-1
chr5_2	exon	27469789	27469968	27469789	ID=Msa0766060-mRNA-1:exon:10751;Parent=Msa0766060-mRNA-1
chr5_2	exon	27469156	27469410	27469156	ID=Msa0766060-mRNA-1:exon:10750;Parent=Msa0766060-mRNA-1
chr5_2	CDS	27472581	27472584	27472581	ID=Msa0766060-mRNA-1:cds;Parent=Msa0766060-mRNA-1
chr5_2	CDS	27471101	27471176	27471101	ID=Msa0766060-mRNA-1:cds;Parent=Msa0766060-mRNA-1
chr5_2	CDS	27471025	27471073	27471025	ID=Msa0766060-mRNA-1:cds;Parent=Msa0766060-mRNA-1
chr5_2	CDS	27470765	27471000	27470765	ID=Msa0766060-mRNA-1:cds;Parent=Msa0766060-mRNA-1
chr5_2	CDS	27470069	27470189	27470069	ID=Msa0766060-mRNA-1:cds;Parent=Msa0766060-mRNA-1
chr5_2	CDS	27469789	27469968	27469789	ID=Msa0766060-mRNA-1:cds;Parent=Msa0766060-mRNA-1
chr5_2	CDS	27469156	27469410	27469156	ID=Msa0766060-mRNA-1:cds;Parent=Msa0766060-mRNA-1

Gene Sequence

>Msa0766060

ATGGGAAAACCCGCTTTAGATTGGAACACAAGAAAGAGGATAGCAATTGGAGCTGCTCGGGGCCTTCTGTATCTTCATGAAGATGTAAAAGCTGCTAATGTGCTTCTGGATGATGATTATGAGGCTATACTCCTTGATCATGCTGATTCCCATGTCACCACTGCTGTACGTGGCATTGTTGGACACATTGCACCGGAGTACCTCTCGACTGGTCAATCATCTGAGAAAACTGATGCGTTTGGATTCGACATTCTCTTATTAGAGCTTATAACTGGAATGACAACCCTTGAGTTTGGAAAAACCTTGAATCAAAAGGGTGCTATGCTAGAGTGGGTAAGTCCATACTCTTCAATCCAAACATTCTTATATAAAGTGGTTGTGCAAGTAGTTTGCGATGGTGACAAGTCTGAATTTTTGTTTGGGGATAAGAAATGTCTTCAAATTATTGGTATCACTGCCCAAGCTCTTAGGAAGATCATGCAAGAGATATATCCAGGACATCTAGACAAAATGCTTGGCCTTGAGCTTGCGCTGCGTGTTAAATATCAGCATTACTATCATCAATCCTCTGTTCTAGGTTTTTCACTTGACCCTGCTGTCATCCGAAGAATTAGGTCTCACCTTCACCCTCGCGAAGTCCAACCACAGGAGCAACAAGCCAATGGGCCAAATATAATTGATTATGTGAAGCTAAATGTTTATGTATTCTCTATTATTACAGTTGGAGGGCTTCTTAATGCAACATGTGGGAATTTTGCTGAGCTGATAATAGCAATATTTGCTCTTAGCAGCAACCAAATTGATGTTGTCAAGTATTTTCTTTTAGGTTCCATCCTTTCAAACCTTCTTCTTGTGCTTGGAACCTCTCTACTTTGTGGTGGCTTTGCAAACCTTGGAGTGGATGACAGAGTAAGTCCCTAA

Protein sequence

>Msa0766060

MGKPALDWNTRKRIAIGAARGLLYLHEDVKAANVLLDDDYEAILLDHADSHVTTAVRGIVGHIAPEYLSTGQSSEKTDAFGFDILLLELITGMTTLEFGKTLNQKGAMLEWVSPYSSIQTFLYKVVVQVVCDGDKSEFLFGDKKCLQIIGITAQALRKIMQEIYPGHLDKMLGLELALRVKYQHYYHQSSVLGFSLDPAVIRRIRSHLHPREVQPQEQQANGPNIIDYVKLNVYVFSIITVGGLLNATCGNFAELIIAIFALSSNQIDVVKYFLLGSILSNLLLVLGTSLLCGGFANLGVDDRVSP*