AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0766230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0766230 MtrunA17_Chr5g0417281 90.541 518 47 2 1 518 1 516 0.0 959
Msa0766230 MtrunA17_Chr5g0436761 53.398 515 177 8 1 510 1 457 1.93e-174 499
Msa0766230 MtrunA17_Chr5g0417211 76.972 317 67 3 1 313 1 315 1.42e-173 495
Msa0766230 MtrunA17_Chr5g0424931 50.000 552 211 10 1 518 8 528 2.74e-164 476
Msa0766230 MtrunA17_Chr2g0289221 69.841 315 90 4 1 312 9 321 1.07e-150 435
Msa0766230 MtrunA17_Chr2g0277461 45.665 519 200 11 4 514 5 449 1.09e-130 387
Msa0766230 MtrunA17_Chr3g0115271 40.526 570 265 13 2 517 9 558 1.30e-121 369
Msa0766230 MtrunA17_Chr1g0178011 50.959 365 162 9 1 354 1 359 1.58e-111 338
Msa0766230 MtrunA17_Chr1g0178011 47.436 78 41 0 426 503 359 436 1.97e-13 72.4
Msa0766230 MtrunA17_Chr5g0424741 40.262 534 237 13 1 469 1 517 6.27e-106 326
Msa0766230 MtrunA17_Chr5g0424801 50.578 346 146 10 1 332 1 335 6.29e-105 319
Msa0766230 MtrunA17_Chr5g0425261 50.289 346 147 9 1 332 1 335 1.18e-102 313
Msa0766230 MtrunA17_Chr4g0028971 37.605 476 210 11 1 414 1 451 2.26e-88 286
Msa0766230 MtrunA17_Chr2g0277531 46.006 313 155 9 6 312 14 318 2.71e-87 271
Msa0766230 MtrunA17_Chr2g0326761 35.165 546 266 15 9 494 8 525 5.32e-87 282
Msa0766230 MtrunA17_Chr1g0181211 33.993 556 280 12 2 494 16 547 3.36e-85 277
Msa0766230 MtrunA17_Chr1g0199681 40.102 394 179 8 9 351 91 478 2.84e-80 264
Msa0766230 MtrunA17_Chr7g0264171 32.743 565 265 16 9 495 28 555 2.59e-76 252
Msa0766230 MtrunA17_Chr7g0264161 39.550 311 157 8 22 312 2 301 1.40e-64 212
Msa0766230 MtrunA17_Chr5g0425661 52.577 194 88 2 12 204 37 227 1.75e-64 209
Msa0766230 MtrunA17_Chr4g0009751 34.133 375 212 9 12 359 19 385 9.06e-61 210
Msa0766230 MtrunA17_Chr2g0282961 34.493 345 192 8 12 332 53 387 6.34e-60 208
Msa0766230 MtrunA17_Chr4g0046371 34.659 352 186 10 12 332 38 376 1.32e-59 209
Msa0766230 MtrunA17_Chr2g0285461 34.545 385 215 12 9 368 20 392 4.34e-58 207
Msa0766230 MtrunA17_Chr8g0346711 35.751 386 210 11 2 360 14 388 2.26e-57 202
Msa0766230 MtrunA17_Chr2g0276781 35.569 343 192 8 12 334 23 356 3.11e-57 202
Msa0766230 MtrunA17_Chr7g0239061 33.807 352 207 8 1 334 3 346 4.00e-57 202
Msa0766230 MtrunA17_Chr5g0433301 33.681 383 213 12 12 367 24 392 1.29e-56 202
Msa0766230 MtrunA17_Chr8g0376771 33.069 378 229 9 9 368 20 391 2.04e-55 199
Msa0766230 MtrunA17_Chr1g0156321 34.734 357 197 11 12 344 43 387 7.58e-55 197
Msa0766230 MtrunA17_Chr8g0385791 33.978 362 213 8 12 354 59 413 1.15e-54 197
Msa0766230 MtrunA17_Chr3g0109391 32.982 379 221 11 12 367 26 394 1.59e-54 196
Msa0766230 MtrunA17_Chr2g0301251 33.149 362 194 8 9 340 20 363 1.67e-54 198
Msa0766230 MtrunA17_Chr4g0070181 33.143 350 214 6 9 342 20 365 2.29e-53 194
Msa0766230 MtrunA17_Chr8g0389081 33.807 352 197 9 9 335 29 369 5.87e-53 193
Msa0766230 MtrunA17_Chr2g0326281 33.684 380 207 14 12 352 56 429 1.55e-51 188
Msa0766230 MtrunA17_Chr4g0029671 34.857 350 192 12 12 332 54 396 1.66e-50 184
Msa0766230 MtrunA17_Chr5g0425651 51.240 121 52 5 230 344 1 120 4.36e-28 108
Msa0766230 MtrunA17_Chr5g0424791 44.595 148 72 4 63 210 14 151 2.28e-27 107
Msa0766230 MtrunA17_Chr8g0356211 26.754 228 147 5 122 335 2 223 2.39e-23 100
Msa0766230 MtrunA17_Chr5g0419861 42.478 113 58 3 53 162 15 123 3.41e-19 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0766230 AT1G77850.1 36.075 535 274 10 9 494 16 531 5.29e-105 326
Msa0766230 AT1G77850.2 36.075 535 274 10 9 494 16 531 8.13e-105 326
Msa0766230 AT2G28350.2 41.379 406 174 9 4 351 2 401 6.40e-87 281
Msa0766230 AT2G28350.1 41.379 406 174 9 4 351 2 401 8.56e-87 282
Msa0766230 AT4G30080.1 36.344 454 219 8 9 411 16 450 1.85e-84 275
Msa0766230 AT4G23980.2 37.500 336 190 8 12 332 12 342 1.21e-63 219
Msa0766230 AT4G23980.1 37.500 336 190 8 12 332 12 342 1.44e-63 219
Msa0766230 AT2G33860.1 35.977 353 186 8 12 337 54 393 6.13e-63 216
Msa0766230 AT5G37020.2 32.891 377 222 9 12 366 22 389 1.94e-59 210
Msa0766230 AT5G37020.1 32.891 377 222 9 12 366 22 389 3.26e-59 210
Msa0766230 AT2G46530.1 36.364 363 199 11 12 355 21 370 1.37e-58 204
Msa0766230 AT2G46530.3 36.364 363 199 11 12 355 42 391 2.52e-58 204
Msa0766230 AT3G61830.1 35.503 338 193 9 12 332 25 354 5.80e-58 203
Msa0766230 AT5G60450.2 36.066 366 208 10 7 349 36 398 7.16e-57 202
Msa0766230 AT5G60450.1 36.288 361 204 10 12 349 65 422 1.91e-56 202
Msa0766230 AT1G35520.1 36.438 365 196 12 12 355 27 376 2.94e-56 198
Msa0766230 AT1G19850.2 34.239 368 205 10 9 347 40 399 3.80e-56 202
Msa0766230 AT1G30330.1 34.711 363 204 11 12 351 23 375 4.16e-56 202
Msa0766230 AT1G19850.1 34.324 370 202 11 9 347 51 410 4.21e-56 202
Msa0766230 AT1G30330.3 34.711 363 204 11 12 351 25 377 5.31e-56 202
Msa0766230 AT1G30330.2 34.711 363 204 11 12 351 25 377 5.31e-56 202
Msa0766230 AT1G34410.1 37.026 343 181 10 12 334 27 354 2.29e-55 196
Msa0766230 AT1G59750.4 34.402 343 196 8 12 334 22 355 2.34e-55 197
Msa0766230 AT1G59750.2 34.402 343 196 8 12 334 22 355 2.83e-55 197
Msa0766230 AT1G59750.1 34.402 343 196 8 12 334 22 355 2.98e-55 197
Msa0766230 AT1G59750.3 34.402 343 196 8 12 334 22 355 2.98e-55 197
Msa0766230 AT1G35540.1 36.628 344 183 10 12 335 27 355 1.89e-54 193
Msa0766230 AT5G62000.2 33.333 366 210 9 12 354 61 415 1.92e-54 197
Msa0766230 AT5G62000.5 33.333 366 210 9 12 354 61 415 1.92e-54 197
Msa0766230 AT5G62000.3 33.333 366 210 9 12 354 61 415 1.92e-54 197
Msa0766230 AT5G62000.1 33.333 366 210 9 12 354 61 415 1.92e-54 197
Msa0766230 AT5G62000.4 33.333 366 210 9 12 354 61 415 2.26e-54 196
Msa0766230 AT1G35240.1 35.277 343 187 9 12 334 27 354 1.16e-53 191
Msa0766230 AT1G34390.1 35.440 364 199 12 12 355 27 374 2.87e-53 190
Msa0766230 AT1G34310.1 37.069 348 184 11 7 334 22 354 7.24e-53 189
Msa0766230 AT1G19220.1 32.486 354 211 7 9 342 20 365 4.29e-52 191
Msa0766230 AT5G20730.1 32.312 359 206 7 9 342 21 367 2.89e-50 186
Msa0766230 AT5G20730.3 32.312 359 206 7 9 342 21 367 3.05e-50 186
Msa0766230 AT5G20730.2 32.591 359 204 8 9 342 21 366 7.25e-50 184
Msa0766230 AT2G33860.2 38.655 238 129 4 114 337 2 236 3.22e-49 176
Msa0766230 AT3G61830.2 35.889 287 164 7 59 332 24 303 1.24e-46 171
Msa0766230 AT1G34170.1 31.232 349 195 10 12 334 25 354 1.78e-46 169
Msa0766230 AT5G37020.3 33.582 268 159 6 114 366 31 294 1.02e-43 165
Msa0766230 AT2G46530.2 37.984 258 139 8 111 355 34 283 3.68e-41 155
Msa0766230 AT1G34170.2 29.799 349 174 11 12 334 25 328 1.70e-38 147
Msa0766230 AT1G34170.3 30.086 349 173 11 12 334 25 328 4.99e-38 147
Msa0766230 AT1G43950.1 36.842 209 112 5 12 213 27 222 8.68e-34 127
Msa0766230 ATMG00940.1 39.252 107 59 3 202 308 44 144 1.50e-15 74.7
Msa0766230 AT4G01500.1 30.000 140 91 3 114 251 36 170 2.31e-11 65.5

Find 135 sgRNAs with CRISPR-Local

Find 332 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGTTTAAACTCTATATT+TGG 0.091140 5_2:+27707133 None:intergenic
CCTGGTTCATTTATGCTATT+TGG 0.118465 5_2:-27706821 Msa0766230:CDS
TCTCAATATCATAGGTTAAA+TGG 0.141063 5_2:-27707351 Msa0766230:intron
GCACAGCACTGCCAGATTTC+TGG 0.173902 5_2:+27708809 None:intergenic
AAAGGCTCCAATGAAATTTA+AGG 0.209057 5_2:-27706734 None:intergenic
TGCTGCATTCAGTGATAAAA+AGG 0.223814 5_2:-27707200 Msa0766230:CDS
CATGTTTACTACTTCCCATT+AGG 0.255048 5_2:-27708752 Msa0766230:CDS
GGGAGAGTGTGTTAGGGTTT+GGG 0.258688 5_2:+27708705 None:intergenic
CACTGCTGGTCCTATATCTT+TGG 0.297607 5_2:-27706930 Msa0766230:CDS
TTTCCTTGGGAATAATTATA+TGG 0.321546 5_2:-27706984 Msa0766230:CDS
GATAAAAGCAATGGTTTCCT+TGG 0.325798 5_2:-27706998 Msa0766230:CDS
AGCATCCGCCATGGACAATT+AGG 0.327846 5_2:+27707933 None:intergenic
TCAAGAAGGGAGAGTGTGTT+AGG 0.330307 5_2:+27708698 None:intergenic
TAAACATGAGAATATAGTTT+TGG 0.333605 5_2:+27708767 None:intergenic
GTGACATTCACGGTAAAGTT+TGG 0.340609 5_2:-27708370 Msa0766230:CDS
GATTTCTGGCTCAACACGGC+TGG 0.344702 5_2:+27708823 None:intergenic
AGGGAGAGTGTGTTAGGGTT+TGG 0.345088 5_2:+27708704 None:intergenic
TTTGATGCCTTAAATTTCAT+TGG 0.347008 5_2:+27706727 None:intergenic
TCTGGAACCTGTGAGACTTT+AGG 0.352969 5_2:+27707322 None:intergenic
ATTGTTCAACAATGTTTGAT+TGG 0.354882 5_2:+27707096 None:intergenic
ATGTTTACTACTTCCCATTA+GGG 0.360944 5_2:-27708751 Msa0766230:CDS
ATGTGTTCTATGTGCCCTAA+TGG 0.363040 5_2:+27708737 None:intergenic
ATGAATATTAAGTGGAATCT+TGG 0.367936 5_2:-27708041 Msa0766230:CDS
GTTTATGGAGACAGTGGAAT+TGG 0.383028 5_2:-27708156 Msa0766230:CDS
CTCAATATCATAGGTTAAAT+GGG 0.383997 5_2:-27707350 Msa0766230:intron
GCCGTGTTGAGCCAGAAATC+TGG 0.395763 5_2:-27708820 Msa0766230:CDS
GAAATCTGGCAGTGCTGTGC+AGG 0.404779 5_2:-27708806 Msa0766230:CDS
AATGTTTAAACTCTATATTT+GGG 0.405482 5_2:+27707134 None:intergenic
AAAGAGATGCTCAAAATTTG+AGG 0.405775 5_2:-27707979 Msa0766230:CDS
GTTCGCCGACAGACAAAGTT+TGG 0.410958 5_2:-27708206 Msa0766230:CDS
CATGATATTTCTAACTCTCT+TGG 0.416099 5_2:-27706890 Msa0766230:CDS
TTCCAGAGCGAGTCAGTCCT+TGG 0.420269 5_2:-27707306 Msa0766230:CDS
CATTGCATCCTCCACAACTT+TGG 0.422061 5_2:+27708061 None:intergenic
GATATAGGACCAGCAGTGTT+TGG 0.430632 5_2:+27706935 None:intergenic
ATGATATTTCTAACTCTCTT+GGG 0.435417 5_2:-27706889 Msa0766230:CDS
GACATCTTCTTACCACTGAT+TGG 0.435417 5_2:-27708313 Msa0766230:CDS
TCTTGGTCTTCACGAACCTT+AGG 0.438896 5_2:+27708545 None:intergenic
TCTCTTGGGACAAAGTTTGC+CGG 0.439539 5_2:-27706875 Msa0766230:CDS
TTGATTGCATGCAGGGAGCC+AGG 0.442279 5_2:-27707063 Msa0766230:CDS
GTGTATTATCCGACGGCTAA+AGG 0.444783 5_2:-27708107 Msa0766230:CDS
ATTAATTTCGCGCATTCCAC+TGG 0.445870 5_2:+27708446 None:intergenic
AGACCAAGATGATGGCAAAA+AGG 0.450916 5_2:-27708531 Msa0766230:CDS
TCCCAAGGACTGACTCGCTC+TGG 0.456814 5_2:+27707304 None:intergenic
GGAGAGTGTGTTAGGGTTTG+GGG 0.461223 5_2:+27708706 None:intergenic
GCTCTTCTTGATTGCATGCA+GGG 0.464239 5_2:-27707070 Msa0766230:CDS
CCGCCATTGTTAGCATCTGA+TGG 0.465747 5_2:+27708482 None:intergenic
CGCCATTGTTAGCATCTGAT+GGG 0.467986 5_2:+27708483 None:intergenic
ATAAAAGCAATGGTTTCCTT+GGG 0.470549 5_2:-27706997 Msa0766230:CDS
CGTATTGCTGTTGTCAGTAC+CGG 0.471275 5_2:+27706856 None:intergenic
GAGACTGTGCAGAGAATGAA+TGG 0.471564 5_2:+27708665 None:intergenic
GGTGACAAGTCCAAAGATAT+AGG 0.472899 5_2:+27706920 None:intergenic
TCCAGAGCGAGTCAGTCCTT+GGG 0.477866 5_2:-27707305 Msa0766230:CDS
TGCTCTTCTTGATTGCATGC+AGG 0.478114 5_2:-27707071 Msa0766230:CDS
AAAGCTTAGAAAATCTGATC+AGG 0.481737 5_2:-27707227 Msa0766230:CDS
CTCCTTCGTCCCAAATATAG+AGG 0.491263 5_2:-27707173 Msa0766230:CDS
TGAGTCTTTCATGAAAACTA+AGG 0.494372 5_2:+27708250 None:intergenic
AGTGTGTTAGGGTTTGGGGA+TGG 0.498039 5_2:+27708710 None:intergenic
TGGGGTGAGAGTCTTGGTAT+AGG 0.502872 5_2:+27708502 None:intergenic
CAAGAGAGTTAGAAATATCA+TGG 0.505101 5_2:+27706891 None:intergenic
ATTTCTGGCTCAACACGGCT+GGG 0.505787 5_2:+27708824 None:intergenic
TTTCAAGAATTAGATATCAT+GGG 0.507765 5_2:-27706770 Msa0766230:CDS
TGGACACCGTTGTTAGTGAC+AGG 0.510488 5_2:+27708581 None:intergenic
TTTATAAATGATAAAAGCAA+TGG 0.512428 5_2:-27707007 Msa0766230:CDS
TCACGAACCTTAGGAGATTG+AGG 0.514142 5_2:+27708554 None:intergenic
TCTTGAAAATCGCTTCCAGC+AGG 0.514221 5_2:+27706785 None:intergenic
GGTAAGATCATAAAACCTGC+TGG 0.523281 5_2:-27706800 Msa0766230:CDS
ATCTGATGGGGTGAGAGTCT+TGG 0.526809 5_2:+27708496 None:intergenic
TGTGTTCTATGTGCCCTAAT+GGG 0.529576 5_2:+27708738 None:intergenic
AATATTAAGTGGAATCTTGG+AGG 0.534264 5_2:-27708038 Msa0766230:CDS
ATCTCCATCAGTGACATTCA+CGG 0.537750 5_2:-27708380 Msa0766230:CDS
AAGAGATGCTCAAAATTTGA+GGG 0.539733 5_2:-27707978 Msa0766230:CDS
ATGGGAAATGATGATTGTAA+AGG 0.540341 5_2:-27706752 Msa0766230:CDS
CCTTAGGAGATTGAGGTTCG+TGG 0.544030 5_2:+27708561 None:intergenic
GTTCAACAATGTTTGATTGG+AGG 0.545186 5_2:+27707099 None:intergenic
CCACGAACCTCAATCTCCTA+AGG 0.545263 5_2:-27708561 Msa0766230:CDS
TTGTCCATGGCGGATGCTTG+AGG 0.546119 5_2:-27707928 Msa0766230:intron
TGGATACAGTGCAAATATGT+CGG 0.548922 5_2:+27707267 None:intergenic
AGGATGCAATGAATATTAAG+TGG 0.550257 5_2:-27708049 Msa0766230:CDS
CAAGAAGGGAGAGTGTGTTA+GGG 0.554864 5_2:+27708699 None:intergenic
TATGGAGACAGTGGAATTGG+CGG 0.554972 5_2:-27708153 Msa0766230:CDS
GGATGAACCTAAAGTCTCAC+AGG 0.555957 5_2:-27707329 Msa0766230:CDS
TGGTGCTGCTGCTAAGATAA+CGG 0.558822 5_2:-27708186 Msa0766230:CDS
GTTGACAAGAAGAAACTAGT+TGG 0.559606 5_2:-27708281 Msa0766230:CDS
AAAGTTGGAAGAAGTTGATG+TGG 0.562830 5_2:+27707027 None:intergenic
CTGATGGAGATTTCTTGAGA+CGG 0.566848 5_2:+27708392 None:intergenic
CGGTGCATTCTCCGTGCCAG+TGG 0.572523 5_2:-27708462 Msa0766230:CDS
ATGGACGGGAACGGTCAAGA+AGG 0.574895 5_2:+27708684 None:intergenic
AGCCTCTATATTTGGGACGA+AGG 0.575856 5_2:+27707171 None:intergenic
GCTGGGTTGCTGAGAAGACA+TGG 0.576060 5_2:+27708841 None:intergenic
TATCTGCTCCTAATTGTCCA+TGG 0.577573 5_2:-27707941 Msa0766230:CDS
TGAAGTTGTGTATTATCCGA+CGG 0.581151 5_2:-27708114 Msa0766230:CDS
CTGTGCAGAGAATGAATGGA+CGG 0.581540 5_2:+27708669 None:intergenic
GTTCGTGAAGACCAAGATGA+TGG 0.585038 5_2:-27708539 Msa0766230:CDS
TAGTTTGACGAAGACTTCAT+CGG 0.586807 5_2:+27708613 None:intergenic
CGAAGACTTCATCGGTGTGA+AGG 0.586907 5_2:+27708621 None:intergenic
AAGTTTCGCCACGTCTACCG+TGG 0.588988 5_2:-27708347 Msa0766230:CDS
AAAATTGTTTATGGAGACAG+TGG 0.589183 5_2:-27708162 Msa0766230:CDS
CTGCCATATAATTATTCCCA+AGG 0.589724 5_2:+27706981 None:intergenic
TTAGAAATATCATGGCTATG+AGG 0.592683 5_2:+27706899 None:intergenic
TGGACGGGAACGGTCAAGAA+GGG 0.593041 5_2:+27708685 None:intergenic
TCAGATGGAAATATCTCAGT+CGG 0.595347 5_2:-27708230 Msa0766230:CDS
TCAAGTGAGCCAAACACTGC+TGG 0.595620 5_2:-27706944 Msa0766230:CDS
TGTCGTTGATGCCAAAGTTG+TGG 0.596764 5_2:-27708072 Msa0766230:CDS
CAGTATTGAGGTCAAGCGGT+GGG 0.600081 5_2:+27708420 None:intergenic
AATACGAAGAAAGTTAGTCC+TGG 0.600138 5_2:-27706839 Msa0766230:CDS
CAGCACCAAACTTTGTCTGT+CGG 0.605931 5_2:+27708201 None:intergenic
TTTACCGTGAATGTCACTGA+TGG 0.607693 5_2:+27708376 None:intergenic
GAAATAGTTTCAACCTCCCA+AGG 0.609478 5_2:+27707289 None:intergenic
CCAAATAGCATAAATGAACC+AGG 0.612115 5_2:+27706821 None:intergenic
ATATTAAGTGGAATCTTGGA+GGG 0.616357 5_2:-27708037 Msa0766230:CDS
TGTGCAGAGAATGAATGGAC+GGG 0.620486 5_2:+27708670 None:intergenic
AGAGAATGAATGGACGGGAA+CGG 0.620696 5_2:+27708675 None:intergenic
ATTATCCGACGGCTAAAGGT+TGG 0.622172 5_2:-27708103 Msa0766230:CDS
AGTGGTAAGAAGATGTCGCA+AGG 0.625494 5_2:+27708319 None:intergenic
AGGGAGTCCCACGGTAGACG+TGG 0.627277 5_2:+27708339 None:intergenic
GACAAGAAGAAACTAGTTGG+CGG 0.628517 5_2:-27708278 Msa0766230:CDS
AGAGCGAGTCAGTCCTTGGG+AGG 0.629409 5_2:-27707302 Msa0766230:CDS
TTACACCAACCTTTAGCCGT+CGG 0.633265 5_2:+27708098 None:intergenic
TATACCTCAAGCATCCGCCA+TGG 0.633522 5_2:+27707924 None:intergenic
AACGAACTCACTCCAATCAG+TGG 0.642790 5_2:+27708301 None:intergenic
CTCACTCCTGTCACTAACAA+CGG 0.648291 5_2:-27708587 Msa0766230:CDS
CATTCTCTGCACAGTCTCCG+CGG 0.651521 5_2:-27708660 Msa0766230:CDS
AGTTTCGCCACGTCTACCGT+GGG 0.652406 5_2:-27708346 Msa0766230:CDS
CGTTGATGCCAAAGTTGTGG+AGG 0.653519 5_2:-27708069 Msa0766230:CDS
TTTCGCGCATTCCACTGGCA+CGG 0.656401 5_2:+27708451 None:intergenic
ACCCCATCAGATGCTAACAA+TGG 0.657432 5_2:-27708485 Msa0766230:CDS
CGTTGTTAGTGACAGGAGTG+AGG 0.661202 5_2:+27708588 None:intergenic
GTGGTAAGAAGATGTCGCAA+GGG 0.664196 5_2:+27708320 None:intergenic
GCCATTGTTAGCATCTGATG+GGG 0.677657 5_2:+27708484 None:intergenic
TCAGTATTGAGGTCAAGCGG+TGG 0.680037 5_2:+27708419 None:intergenic
GCCAGATTTCTGGCTCAACA+CGG 0.683376 5_2:+27708819 None:intergenic
CCATCAGATGCTAACAATGG+CGG 0.695708 5_2:-27708482 Msa0766230:CDS
GATGTCGCAAGGGAGTCCCA+CGG 0.712482 5_2:+27708330 None:intergenic
CTGCTCCTAATTGTCCATGG+CGG 0.722372 5_2:-27707938 Msa0766230:CDS
GTCAGCGAGTAAATCAACCG+CGG 0.752817 5_2:+27708643 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTAACTTTCTTTTTT+AGG - chr5_2:27707796-27707815 Msa0766230:intron 10.0%
!!! TTAATTAACTTTCTTTTTTA+GGG - chr5_2:27707797-27707816 Msa0766230:intron 10.0%
!!! TTTAATTAACTTTCTTTTTT+AGG - chr5_2:27707796-27707815 Msa0766230:intron 10.0%
!!! TTAATTAACTTTCTTTTTTA+GGG - chr5_2:27707797-27707816 Msa0766230:intron 10.0%
!!! ATATAGTTTTGGAATTTTAA+CGG + chr5_2:27706800-27706819 None:intergenic 15.0%
!!! GTATCAATCATTTAAAATAT+AGG + chr5_2:27708184-27708203 None:intergenic 15.0%
!!! ATATTTTAAATGATTGATAC+TGG - chr5_2:27708184-27708203 Msa0766230:CDS 15.0%
!! AATGTTTAAACTCTATATTT+GGG + chr5_2:27708444-27708463 None:intergenic 15.0%
!! AAATGTTTAAACTCTATATT+TGG + chr5_2:27708445-27708464 None:intergenic 15.0%
!! TTTATAAATGATAAAAGCAA+TGG - chr5_2:27708568-27708587 Msa0766230:CDS 15.0%
!!! ATATAGTTTTGGAATTTTAA+CGG + chr5_2:27706800-27706819 None:intergenic 15.0%
!!! GTATCAATCATTTAAAATAT+AGG + chr5_2:27708184-27708203 None:intergenic 15.0%
!!! ATATTTTAAATGATTGATAC+TGG - chr5_2:27708184-27708203 Msa0766230:CDS 15.0%
!! AATGTTTAAACTCTATATTT+GGG + chr5_2:27708444-27708463 None:intergenic 15.0%
!! AAATGTTTAAACTCTATATT+TGG + chr5_2:27708445-27708464 None:intergenic 15.0%
!! TTTATAAATGATAAAAGCAA+TGG - chr5_2:27708568-27708587 Msa0766230:CDS 15.0%
!!! TAAACATGAGAATATAGTTT+TGG + chr5_2:27706811-27706830 None:intergenic 20.0%
!!! CTACTTAATATTTTTACAAC+AGG + chr5_2:27708116-27708135 None:intergenic 20.0%
!! CTGTTGTAAAAATATTAAGT+AGG - chr5_2:27708114-27708133 Msa0766230:CDS 20.0%
!!! ATTTTTCATCTCAATATCAT+AGG - chr5_2:27708216-27708235 Msa0766230:CDS 20.0%
!!! GCTTTTATCATTTATAAAGT+TGG + chr5_2:27708566-27708585 None:intergenic 20.0%
!!! TTTTCAAGAATTAGATATCA+TGG - chr5_2:27708804-27708823 Msa0766230:CDS 20.0%
!! TTTCAAGAATTAGATATCAT+GGG - chr5_2:27708805-27708824 Msa0766230:CDS 20.0%
!!! TAAACATGAGAATATAGTTT+TGG + chr5_2:27706811-27706830 None:intergenic 20.0%
!!! CTACTTAATATTTTTACAAC+AGG + chr5_2:27708116-27708135 None:intergenic 20.0%
!! CTGTTGTAAAAATATTAAGT+AGG - chr5_2:27708114-27708133 Msa0766230:CDS 20.0%
!!! ATTTTTCATCTCAATATCAT+AGG - chr5_2:27708216-27708235 Msa0766230:CDS 20.0%
!!! GCTTTTATCATTTATAAAGT+TGG + chr5_2:27708566-27708585 None:intergenic 20.0%
!!! TTTTCAAGAATTAGATATCA+TGG - chr5_2:27708804-27708823 Msa0766230:CDS 20.0%
!! TTTCAAGAATTAGATATCAT+GGG - chr5_2:27708805-27708824 Msa0766230:CDS 20.0%
!!! GATAACGGAAAAATTGTTTA+TGG - chr5_2:27707404-27707423 Msa0766230:intron 25.0%
! ATGAATATTAAGTGGAATCT+TGG - chr5_2:27707534-27707553 Msa0766230:intron 25.0%
!! TATGATGCAATTCTTTAAGA+AGG - chr5_2:27707889-27707908 Msa0766230:intron 25.0%
!! ATTCTTTAAGAAGGTGAAAT+AGG - chr5_2:27707898-27707917 Msa0766230:intron 25.0%
! TATGTTTACATTCGTACTAT+AGG - chr5_2:27707941-27707960 Msa0766230:CDS 25.0%
! TTCTTAGGAAATATCACTAA+TGG + chr5_2:27708068-27708087 None:intergenic 25.0%
! TAGTGATATTTCCTAAGAAA+CGG - chr5_2:27708069-27708088 Msa0766230:CDS 25.0%
! TCTCAATATCATAGGTTAAA+TGG - chr5_2:27708224-27708243 Msa0766230:CDS 25.0%
! CTCAATATCATAGGTTAAAT+GGG - chr5_2:27708225-27708244 Msa0766230:CDS 25.0%
!!! ATTTTCTAAGCTTTTTTGTC+GGG + chr5_2:27708342-27708361 None:intergenic 25.0%
!!! GATTTTCTAAGCTTTTTTGT+CGG + chr5_2:27708343-27708362 None:intergenic 25.0%
! TCAAAAAAGCCTCTATATTT+GGG + chr5_2:27708414-27708433 None:intergenic 25.0%
! TTCAAAAAAGCCTCTATATT+TGG + chr5_2:27708415-27708434 None:intergenic 25.0%
! ATTGTTCAACAATGTTTGAT+TGG + chr5_2:27708482-27708501 None:intergenic 25.0%
! TTTCCTTGGGAATAATTATA+TGG - chr5_2:27708591-27708610 Msa0766230:CDS 25.0%
!! ATGATATTTCTAACTCTCTT+GGG - chr5_2:27708686-27708705 Msa0766230:CDS 25.0%
!!! GATAACGGAAAAATTGTTTA+TGG - chr5_2:27707404-27707423 Msa0766230:intron 25.0%
! ATGAATATTAAGTGGAATCT+TGG - chr5_2:27707534-27707553 Msa0766230:intron 25.0%
!! TATGATGCAATTCTTTAAGA+AGG - chr5_2:27707889-27707908 Msa0766230:intron 25.0%
!! ATTCTTTAAGAAGGTGAAAT+AGG - chr5_2:27707898-27707917 Msa0766230:intron 25.0%
! TATGTTTACATTCGTACTAT+AGG - chr5_2:27707941-27707960 Msa0766230:CDS 25.0%
! TTCTTAGGAAATATCACTAA+TGG + chr5_2:27708068-27708087 None:intergenic 25.0%
! TAGTGATATTTCCTAAGAAA+CGG - chr5_2:27708069-27708088 Msa0766230:CDS 25.0%
! TCTCAATATCATAGGTTAAA+TGG - chr5_2:27708224-27708243 Msa0766230:CDS 25.0%
! CTCAATATCATAGGTTAAAT+GGG - chr5_2:27708225-27708244 Msa0766230:CDS 25.0%
!!! ATTTTCTAAGCTTTTTTGTC+GGG + chr5_2:27708342-27708361 None:intergenic 25.0%
!!! GATTTTCTAAGCTTTTTTGT+CGG + chr5_2:27708343-27708362 None:intergenic 25.0%
! TCAAAAAAGCCTCTATATTT+GGG + chr5_2:27708414-27708433 None:intergenic 25.0%
! TTCAAAAAAGCCTCTATATT+TGG + chr5_2:27708415-27708434 None:intergenic 25.0%
! ATTGTTCAACAATGTTTGAT+TGG + chr5_2:27708482-27708501 None:intergenic 25.0%
! TTTCCTTGGGAATAATTATA+TGG - chr5_2:27708591-27708610 Msa0766230:CDS 25.0%
!! ATGATATTTCTAACTCTCTT+GGG - chr5_2:27708686-27708705 Msa0766230:CDS 25.0%
ATGTTTACTACTTCCCATTA+GGG - chr5_2:27706824-27706843 Msa0766230:CDS 30.0%
TGAGTCTTTCATGAAAACTA+AGG + chr5_2:27707328-27707347 None:intergenic 30.0%
AAAATTGTTTATGGAGACAG+TGG - chr5_2:27707413-27707432 Msa0766230:intron 30.0%
AGGATGCAATGAATATTAAG+TGG - chr5_2:27707526-27707545 Msa0766230:intron 30.0%
AATATTAAGTGGAATCTTGG+AGG - chr5_2:27707537-27707556 Msa0766230:intron 30.0%
ATATTAAGTGGAATCTTGGA+GGG - chr5_2:27707538-27707557 Msa0766230:intron 30.0%
AAAGAGATGCTCAAAATTTG+AGG - chr5_2:27707596-27707615 Msa0766230:intron 30.0%
AAGAGATGCTCAAAATTTGA+GGG - chr5_2:27707597-27707616 Msa0766230:intron 30.0%
AAGTTTCTTAACCGTTTCTT+AGG + chr5_2:27708083-27708102 None:intergenic 30.0%
AAAGCTTAGAAAATCTGATC+AGG - chr5_2:27708348-27708367 Msa0766230:CDS 30.0%
!!! TATAGAGGCTTTTTTGAAGA+TGG - chr5_2:27708417-27708436 Msa0766230:CDS 30.0%
!! AAGTTGATGTGGAAAAAAGA+CGG + chr5_2:27708540-27708559 None:intergenic 30.0%
ATAAAAGCAATGGTTTCCTT+GGG - chr5_2:27708578-27708597 Msa0766230:CDS 30.0%
TTAGAAATATCATGGCTATG+AGG + chr5_2:27708679-27708698 None:intergenic 30.0%
CAAGAGAGTTAGAAATATCA+TGG + chr5_2:27708687-27708706 None:intergenic 30.0%
! CATGATATTTCTAACTCTCT+TGG - chr5_2:27708685-27708704 Msa0766230:CDS 30.0%
ATGGGAAATGATGATTGTAA+AGG - chr5_2:27708823-27708842 Msa0766230:CDS 30.0%
ATGTTTACTACTTCCCATTA+GGG - chr5_2:27706824-27706843 Msa0766230:CDS 30.0%
TGAGTCTTTCATGAAAACTA+AGG + chr5_2:27707328-27707347 None:intergenic 30.0%
AAAATTGTTTATGGAGACAG+TGG - chr5_2:27707413-27707432 Msa0766230:intron 30.0%
AGGATGCAATGAATATTAAG+TGG - chr5_2:27707526-27707545 Msa0766230:intron 30.0%
AATATTAAGTGGAATCTTGG+AGG - chr5_2:27707537-27707556 Msa0766230:intron 30.0%
ATATTAAGTGGAATCTTGGA+GGG - chr5_2:27707538-27707557 Msa0766230:intron 30.0%
AAAGAGATGCTCAAAATTTG+AGG - chr5_2:27707596-27707615 Msa0766230:intron 30.0%
AAGAGATGCTCAAAATTTGA+GGG - chr5_2:27707597-27707616 Msa0766230:intron 30.0%
AAGTTTCTTAACCGTTTCTT+AGG + chr5_2:27708083-27708102 None:intergenic 30.0%
AAAGCTTAGAAAATCTGATC+AGG - chr5_2:27708348-27708367 Msa0766230:CDS 30.0%
!!! TATAGAGGCTTTTTTGAAGA+TGG - chr5_2:27708417-27708436 Msa0766230:CDS 30.0%
!! AAGTTGATGTGGAAAAAAGA+CGG + chr5_2:27708540-27708559 None:intergenic 30.0%
ATAAAAGCAATGGTTTCCTT+GGG - chr5_2:27708578-27708597 Msa0766230:CDS 30.0%
TTAGAAATATCATGGCTATG+AGG + chr5_2:27708679-27708698 None:intergenic 30.0%
CAAGAGAGTTAGAAATATCA+TGG + chr5_2:27708687-27708706 None:intergenic 30.0%
! CATGATATTTCTAACTCTCT+TGG - chr5_2:27708685-27708704 Msa0766230:CDS 30.0%
ATGGGAAATGATGATTGTAA+AGG - chr5_2:27708823-27708842 Msa0766230:CDS 30.0%
CATGTTTACTACTTCCCATT+AGG - chr5_2:27706823-27706842 Msa0766230:CDS 35.0%
TAGTTTGACGAAGACTTCAT+CGG + chr5_2:27706965-27706984 None:intergenic 35.0%
!! TTTTCAGTATTGAGGTCAAG+CGG + chr5_2:27707162-27707181 None:intergenic 35.0%
GTTGACAAGAAGAAACTAGT+TGG - chr5_2:27707294-27707313 Msa0766230:CDS 35.0%
! GTTTTCATGAAAGACTCAGA+TGG - chr5_2:27707330-27707349 Msa0766230:CDS 35.0%
TCAGATGGAAATATCTCAGT+CGG - chr5_2:27707345-27707364 Msa0766230:intron 35.0%
TGAAGTTGTGTATTATCCGA+CGG - chr5_2:27707461-27707480 Msa0766230:intron 35.0%
CATACACTGAGACAACTTAT+TGG + chr5_2:27707925-27707944 None:intergenic 35.0%
AGTATGCATCTACAAGTCAA+AGG + chr5_2:27708158-27708177 None:intergenic 35.0%
TGGATACAGTGCAAATATGT+CGG + chr5_2:27708311-27708330 None:intergenic 35.0%
TGCTGCATTCAGTGATAAAA+AGG - chr5_2:27708375-27708394 Msa0766230:CDS 35.0%
GCTGCATTCAGTGATAAAAA+GGG - chr5_2:27708376-27708395 Msa0766230:CDS 35.0%
GTTCAACAATGTTTGATTGG+AGG + chr5_2:27708479-27708498 None:intergenic 35.0%
!! AAAGTTGGAAGAAGTTGATG+TGG + chr5_2:27708551-27708570 None:intergenic 35.0%
GATAAAAGCAATGGTTTCCT+TGG - chr5_2:27708577-27708596 Msa0766230:CDS 35.0%
CTGCCATATAATTATTCCCA+AGG + chr5_2:27708597-27708616 None:intergenic 35.0%
AATACGAAGAAAGTTAGTCC+TGG - chr5_2:27708736-27708755 Msa0766230:CDS 35.0%
CCAAATAGCATAAATGAACC+AGG + chr5_2:27708757-27708776 None:intergenic 35.0%
CCTGGTTCATTTATGCTATT+TGG - chr5_2:27708754-27708773 Msa0766230:CDS 35.0%
CATGTTTACTACTTCCCATT+AGG - chr5_2:27706823-27706842 Msa0766230:CDS 35.0%
TAGTTTGACGAAGACTTCAT+CGG + chr5_2:27706965-27706984 None:intergenic 35.0%
!! TTTTCAGTATTGAGGTCAAG+CGG + chr5_2:27707162-27707181 None:intergenic 35.0%
GTTGACAAGAAGAAACTAGT+TGG - chr5_2:27707294-27707313 Msa0766230:CDS 35.0%
! GTTTTCATGAAAGACTCAGA+TGG - chr5_2:27707330-27707349 Msa0766230:CDS 35.0%
TCAGATGGAAATATCTCAGT+CGG - chr5_2:27707345-27707364 Msa0766230:intron 35.0%
TGAAGTTGTGTATTATCCGA+CGG - chr5_2:27707461-27707480 Msa0766230:intron 35.0%
CATACACTGAGACAACTTAT+TGG + chr5_2:27707925-27707944 None:intergenic 35.0%
AGTATGCATCTACAAGTCAA+AGG + chr5_2:27708158-27708177 None:intergenic 35.0%
TGGATACAGTGCAAATATGT+CGG + chr5_2:27708311-27708330 None:intergenic 35.0%
TGCTGCATTCAGTGATAAAA+AGG - chr5_2:27708375-27708394 Msa0766230:CDS 35.0%
GCTGCATTCAGTGATAAAAA+GGG - chr5_2:27708376-27708395 Msa0766230:CDS 35.0%
GTTCAACAATGTTTGATTGG+AGG + chr5_2:27708479-27708498 None:intergenic 35.0%
!! AAAGTTGGAAGAAGTTGATG+TGG + chr5_2:27708551-27708570 None:intergenic 35.0%
GATAAAAGCAATGGTTTCCT+TGG - chr5_2:27708577-27708596 Msa0766230:CDS 35.0%
CTGCCATATAATTATTCCCA+AGG + chr5_2:27708597-27708616 None:intergenic 35.0%
AATACGAAGAAAGTTAGTCC+TGG - chr5_2:27708736-27708755 Msa0766230:CDS 35.0%
CCAAATAGCATAAATGAACC+AGG + chr5_2:27708757-27708776 None:intergenic 35.0%
CCTGGTTCATTTATGCTATT+TGG - chr5_2:27708754-27708773 Msa0766230:CDS 35.0%
TGTGTTCTATGTGCCCTAAT+GGG + chr5_2:27706840-27706859 None:intergenic 40.0%
ATGTGTTCTATGTGCCCTAA+TGG + chr5_2:27706841-27706860 None:intergenic 40.0%
! AGACCAAGATGATGGCAAAA+AGG - chr5_2:27707044-27707063 Msa0766230:CDS 40.0%
! CAACCTTTTTGCCATCATCT+TGG + chr5_2:27707050-27707069 None:intergenic 40.0%
ATTAATTTCGCGCATTCCAC+TGG + chr5_2:27707132-27707151 None:intergenic 40.0%
CTGATGGAGATTTCTTGAGA+CGG + chr5_2:27707186-27707205 None:intergenic 40.0%
ATCTCCATCAGTGACATTCA+CGG - chr5_2:27707195-27707214 Msa0766230:CDS 40.0%
TTTACCGTGAATGTCACTGA+TGG + chr5_2:27707202-27707221 None:intergenic 40.0%
GTGACATTCACGGTAAAGTT+TGG - chr5_2:27707205-27707224 Msa0766230:CDS 40.0%
GACATCTTCTTACCACTGAT+TGG - chr5_2:27707262-27707281 Msa0766230:CDS 40.0%
GACAAGAAGAAACTAGTTGG+CGG - chr5_2:27707297-27707316 Msa0766230:CDS 40.0%
GTTTATGGAGACAGTGGAAT+TGG - chr5_2:27707419-27707438 Msa0766230:intron 40.0%
! GGAATTGGCGGATAAAAACA+TGG - chr5_2:27707434-27707453 Msa0766230:intron 40.0%
TATCTGCTCCTAATTGTCCA+TGG - chr5_2:27707634-27707653 Msa0766230:intron 40.0%
GAAATAGTTTCAACCTCCCA+AGG + chr5_2:27708289-27708308 None:intergenic 40.0%
!!! TTTTTTGTCGGGTGGAATTG+TGG + chr5_2:27708331-27708350 None:intergenic 40.0%
!!! TTCTAAGCTTTTTTGTCGGG+TGG + chr5_2:27708339-27708358 None:intergenic 40.0%
GGTGACAAGTCCAAAGATAT+AGG + chr5_2:27708658-27708677 None:intergenic 40.0%
GGTAAGATCATAAAACCTGC+TGG - chr5_2:27708775-27708794 Msa0766230:CDS 40.0%
TGTGTTCTATGTGCCCTAAT+GGG + chr5_2:27706840-27706859 None:intergenic 40.0%
ATGTGTTCTATGTGCCCTAA+TGG + chr5_2:27706841-27706860 None:intergenic 40.0%
! AGACCAAGATGATGGCAAAA+AGG - chr5_2:27707044-27707063 Msa0766230:CDS 40.0%
! CAACCTTTTTGCCATCATCT+TGG + chr5_2:27707050-27707069 None:intergenic 40.0%
ATTAATTTCGCGCATTCCAC+TGG + chr5_2:27707132-27707151 None:intergenic 40.0%
CTGATGGAGATTTCTTGAGA+CGG + chr5_2:27707186-27707205 None:intergenic 40.0%
ATCTCCATCAGTGACATTCA+CGG - chr5_2:27707195-27707214 Msa0766230:CDS 40.0%
TTTACCGTGAATGTCACTGA+TGG + chr5_2:27707202-27707221 None:intergenic 40.0%
GTGACATTCACGGTAAAGTT+TGG - chr5_2:27707205-27707224 Msa0766230:CDS 40.0%
GACATCTTCTTACCACTGAT+TGG - chr5_2:27707262-27707281 Msa0766230:CDS 40.0%
GACAAGAAGAAACTAGTTGG+CGG - chr5_2:27707297-27707316 Msa0766230:CDS 40.0%
GTTTATGGAGACAGTGGAAT+TGG - chr5_2:27707419-27707438 Msa0766230:intron 40.0%
! GGAATTGGCGGATAAAAACA+TGG - chr5_2:27707434-27707453 Msa0766230:intron 40.0%
TATCTGCTCCTAATTGTCCA+TGG - chr5_2:27707634-27707653 Msa0766230:intron 40.0%
GAAATAGTTTCAACCTCCCA+AGG + chr5_2:27708289-27708308 None:intergenic 40.0%
!!! TTTTTTGTCGGGTGGAATTG+TGG + chr5_2:27708331-27708350 None:intergenic 40.0%
!!! TTCTAAGCTTTTTTGTCGGG+TGG + chr5_2:27708339-27708358 None:intergenic 40.0%
GGTGACAAGTCCAAAGATAT+AGG + chr5_2:27708658-27708677 None:intergenic 40.0%
GGTAAGATCATAAAACCTGC+TGG - chr5_2:27708775-27708794 Msa0766230:CDS 40.0%
! CAAGAAGGGAGAGTGTGTTA+GGG + chr5_2:27706879-27706898 None:intergenic 45.0%
! TCAAGAAGGGAGAGTGTGTT+AGG + chr5_2:27706880-27706899 None:intergenic 45.0%
AGAGAATGAATGGACGGGAA+CGG + chr5_2:27706903-27706922 None:intergenic 45.0%
TGTGCAGAGAATGAATGGAC+GGG + chr5_2:27706908-27706927 None:intergenic 45.0%
CTGTGCAGAGAATGAATGGA+CGG + chr5_2:27706909-27706928 None:intergenic 45.0%
GAGACTGTGCAGAGAATGAA+TGG + chr5_2:27706913-27706932 None:intergenic 45.0%
CTCACTCCTGTCACTAACAA+CGG - chr5_2:27706988-27707007 Msa0766230:CDS 45.0%
TCACGAACCTTAGGAGATTG+AGG + chr5_2:27707024-27707043 None:intergenic 45.0%
! TCTTGGTCTTCACGAACCTT+AGG + chr5_2:27707033-27707052 None:intergenic 45.0%
GTTCGTGAAGACCAAGATGA+TGG - chr5_2:27707036-27707055 Msa0766230:CDS 45.0%
ACCCCATCAGATGCTAACAA+TGG - chr5_2:27707090-27707109 Msa0766230:CDS 45.0%
GCCATTGTTAGCATCTGATG+GGG + chr5_2:27707094-27707113 None:intergenic 45.0%
CGCCATTGTTAGCATCTGAT+GGG + chr5_2:27707095-27707114 None:intergenic 45.0%
CCATCAGATGCTAACAATGG+CGG - chr5_2:27707093-27707112 Msa0766230:CDS 45.0%
! GAGACGGCTTTTCAGTATTG+AGG + chr5_2:27707170-27707189 None:intergenic 45.0%
GTGGTAAGAAGATGTCGCAA+GGG + chr5_2:27707258-27707277 None:intergenic 45.0%
AGTGGTAAGAAGATGTCGCA+AGG + chr5_2:27707259-27707278 None:intergenic 45.0%
AACGAACTCACTCCAATCAG+TGG + chr5_2:27707277-27707296 None:intergenic 45.0%
CAGCACCAAACTTTGTCTGT+CGG + chr5_2:27707377-27707396 None:intergenic 45.0%
!! TGGTGCTGCTGCTAAGATAA+CGG - chr5_2:27707389-27707408 Msa0766230:intron 45.0%
TATGGAGACAGTGGAATTGG+CGG - chr5_2:27707422-27707441 Msa0766230:intron 45.0%
GTGTATTATCCGACGGCTAA+AGG - chr5_2:27707468-27707487 Msa0766230:intron 45.0%
ATTATCCGACGGCTAAAGGT+TGG - chr5_2:27707472-27707491 Msa0766230:intron 45.0%
TTACACCAACCTTTAGCCGT+CGG + chr5_2:27707480-27707499 None:intergenic 45.0%
TGTCGTTGATGCCAAAGTTG+TGG - chr5_2:27707503-27707522 Msa0766230:intron 45.0%
CATTGCATCCTCCACAACTT+TGG + chr5_2:27707517-27707536 None:intergenic 45.0%
GGATGAACCTAAAGTCTCAC+AGG - chr5_2:27708246-27708265 Msa0766230:CDS 45.0%
! TCTGGAACCTGTGAGACTTT+AGG + chr5_2:27708256-27708275 None:intergenic 45.0%
CTCCTTCGTCCCAAATATAG+AGG - chr5_2:27708402-27708421 Msa0766230:CDS 45.0%
! AGCCTCTATATTTGGGACGA+AGG + chr5_2:27708407-27708426 None:intergenic 45.0%
TGCTCTTCTTGATTGCATGC+AGG - chr5_2:27708504-27708523 Msa0766230:CDS 45.0%
GCTCTTCTTGATTGCATGCA+GGG - chr5_2:27708505-27708524 Msa0766230:CDS 45.0%
GATATAGGACCAGCAGTGTT+TGG + chr5_2:27708643-27708662 None:intergenic 45.0%
CACTGCTGGTCCTATATCTT+TGG - chr5_2:27708645-27708664 Msa0766230:CDS 45.0%
TCTCTTGGGACAAAGTTTGC+CGG - chr5_2:27708700-27708719 Msa0766230:CDS 45.0%
! CGTATTGCTGTTGTCAGTAC+CGG + chr5_2:27708722-27708741 None:intergenic 45.0%
TCTTGAAAATCGCTTCCAGC+AGG + chr5_2:27708793-27708812 None:intergenic 45.0%
! CAAGAAGGGAGAGTGTGTTA+GGG + chr5_2:27706879-27706898 None:intergenic 45.0%
! TCAAGAAGGGAGAGTGTGTT+AGG + chr5_2:27706880-27706899 None:intergenic 45.0%
AGAGAATGAATGGACGGGAA+CGG + chr5_2:27706903-27706922 None:intergenic 45.0%
TGTGCAGAGAATGAATGGAC+GGG + chr5_2:27706908-27706927 None:intergenic 45.0%
CTGTGCAGAGAATGAATGGA+CGG + chr5_2:27706909-27706928 None:intergenic 45.0%
GAGACTGTGCAGAGAATGAA+TGG + chr5_2:27706913-27706932 None:intergenic 45.0%
CTCACTCCTGTCACTAACAA+CGG - chr5_2:27706988-27707007 Msa0766230:CDS 45.0%
TCACGAACCTTAGGAGATTG+AGG + chr5_2:27707024-27707043 None:intergenic 45.0%
! TCTTGGTCTTCACGAACCTT+AGG + chr5_2:27707033-27707052 None:intergenic 45.0%
GTTCGTGAAGACCAAGATGA+TGG - chr5_2:27707036-27707055 Msa0766230:CDS 45.0%
ACCCCATCAGATGCTAACAA+TGG - chr5_2:27707090-27707109 Msa0766230:CDS 45.0%
GCCATTGTTAGCATCTGATG+GGG + chr5_2:27707094-27707113 None:intergenic 45.0%
CGCCATTGTTAGCATCTGAT+GGG + chr5_2:27707095-27707114 None:intergenic 45.0%
CCATCAGATGCTAACAATGG+CGG - chr5_2:27707093-27707112 Msa0766230:CDS 45.0%
! GAGACGGCTTTTCAGTATTG+AGG + chr5_2:27707170-27707189 None:intergenic 45.0%
GTGGTAAGAAGATGTCGCAA+GGG + chr5_2:27707258-27707277 None:intergenic 45.0%
AGTGGTAAGAAGATGTCGCA+AGG + chr5_2:27707259-27707278 None:intergenic 45.0%
AACGAACTCACTCCAATCAG+TGG + chr5_2:27707277-27707296 None:intergenic 45.0%
CAGCACCAAACTTTGTCTGT+CGG + chr5_2:27707377-27707396 None:intergenic 45.0%
!! TGGTGCTGCTGCTAAGATAA+CGG - chr5_2:27707389-27707408 Msa0766230:intron 45.0%
TATGGAGACAGTGGAATTGG+CGG - chr5_2:27707422-27707441 Msa0766230:intron 45.0%
GTGTATTATCCGACGGCTAA+AGG - chr5_2:27707468-27707487 Msa0766230:intron 45.0%
ATTATCCGACGGCTAAAGGT+TGG - chr5_2:27707472-27707491 Msa0766230:intron 45.0%
TTACACCAACCTTTAGCCGT+CGG + chr5_2:27707480-27707499 None:intergenic 45.0%
TGTCGTTGATGCCAAAGTTG+TGG - chr5_2:27707503-27707522 Msa0766230:intron 45.0%
CATTGCATCCTCCACAACTT+TGG + chr5_2:27707517-27707536 None:intergenic 45.0%
GGATGAACCTAAAGTCTCAC+AGG - chr5_2:27708246-27708265 Msa0766230:CDS 45.0%
! TCTGGAACCTGTGAGACTTT+AGG + chr5_2:27708256-27708275 None:intergenic 45.0%
CTCCTTCGTCCCAAATATAG+AGG - chr5_2:27708402-27708421 Msa0766230:CDS 45.0%
! AGCCTCTATATTTGGGACGA+AGG + chr5_2:27708407-27708426 None:intergenic 45.0%
TGCTCTTCTTGATTGCATGC+AGG - chr5_2:27708504-27708523 Msa0766230:CDS 45.0%
GCTCTTCTTGATTGCATGCA+GGG - chr5_2:27708505-27708524 Msa0766230:CDS 45.0%
GATATAGGACCAGCAGTGTT+TGG + chr5_2:27708643-27708662 None:intergenic 45.0%
CACTGCTGGTCCTATATCTT+TGG - chr5_2:27708645-27708664 Msa0766230:CDS 45.0%
TCTCTTGGGACAAAGTTTGC+CGG - chr5_2:27708700-27708719 Msa0766230:CDS 45.0%
! CGTATTGCTGTTGTCAGTAC+CGG + chr5_2:27708722-27708741 None:intergenic 45.0%
TCTTGAAAATCGCTTCCAGC+AGG + chr5_2:27708793-27708812 None:intergenic 45.0%
!! ATTTCTGGCTCAACACGGCT+GGG + chr5_2:27706754-27706773 None:intergenic 50.0%
!! GCCAGATTTCTGGCTCAACA+CGG + chr5_2:27706759-27706778 None:intergenic 50.0%
AGTGTGTTAGGGTTTGGGGA+TGG + chr5_2:27706868-27706887 None:intergenic 50.0%
! GGAGAGTGTGTTAGGGTTTG+GGG + chr5_2:27706872-27706891 None:intergenic 50.0%
! GGGAGAGTGTGTTAGGGTTT+GGG + chr5_2:27706873-27706892 None:intergenic 50.0%
! AGGGAGAGTGTGTTAGGGTT+TGG + chr5_2:27706874-27706893 None:intergenic 50.0%
GTCAGCGAGTAAATCAACCG+CGG + chr5_2:27706935-27706954 None:intergenic 50.0%
CGAAGACTTCATCGGTGTGA+AGG + chr5_2:27706957-27706976 None:intergenic 50.0%
CGTTGTTAGTGACAGGAGTG+AGG + chr5_2:27706990-27707009 None:intergenic 50.0%
TGGACACCGTTGTTAGTGAC+AGG + chr5_2:27706997-27707016 None:intergenic 50.0%
CCTTAGGAGATTGAGGTTCG+TGG + chr5_2:27707017-27707036 None:intergenic 50.0%
CCACGAACCTCAATCTCCTA+AGG - chr5_2:27707014-27707033 Msa0766230:CDS 50.0%
TGGGGTGAGAGTCTTGGTAT+AGG + chr5_2:27707076-27707095 None:intergenic 50.0%
ATCTGATGGGGTGAGAGTCT+TGG + chr5_2:27707082-27707101 None:intergenic 50.0%
CCGCCATTGTTAGCATCTGA+TGG + chr5_2:27707096-27707115 None:intergenic 50.0%
! CAGTATTGAGGTCAAGCGGT+GGG + chr5_2:27707158-27707177 None:intergenic 50.0%
! TCAGTATTGAGGTCAAGCGG+TGG + chr5_2:27707159-27707178 None:intergenic 50.0%
GTTCGCCGACAGACAAAGTT+TGG - chr5_2:27707369-27707388 Msa0766230:intron 50.0%
CGTTGATGCCAAAGTTGTGG+AGG - chr5_2:27707506-27707525 Msa0766230:intron 50.0%
CTGCTCCTAATTGTCCATGG+CGG - chr5_2:27707637-27707656 Msa0766230:intron 50.0%
AGCATCCGCCATGGACAATT+AGG + chr5_2:27707645-27707664 None:intergenic 50.0%
TATACCTCAAGCATCCGCCA+TGG + chr5_2:27707654-27707673 None:intergenic 50.0%
TGTGGAAAAAAGACGGTGCC+TGG + chr5_2:27708533-27708552 None:intergenic 50.0%
TCAAGTGAGCCAAACACTGC+TGG - chr5_2:27708631-27708650 Msa0766230:CDS 50.0%
!! ATTTCTGGCTCAACACGGCT+GGG + chr5_2:27706754-27706773 None:intergenic 50.0%
!! GCCAGATTTCTGGCTCAACA+CGG + chr5_2:27706759-27706778 None:intergenic 50.0%
AGTGTGTTAGGGTTTGGGGA+TGG + chr5_2:27706868-27706887 None:intergenic 50.0%
! GGAGAGTGTGTTAGGGTTTG+GGG + chr5_2:27706872-27706891 None:intergenic 50.0%
! GGGAGAGTGTGTTAGGGTTT+GGG + chr5_2:27706873-27706892 None:intergenic 50.0%
! AGGGAGAGTGTGTTAGGGTT+TGG + chr5_2:27706874-27706893 None:intergenic 50.0%
GTCAGCGAGTAAATCAACCG+CGG + chr5_2:27706935-27706954 None:intergenic 50.0%
CGAAGACTTCATCGGTGTGA+AGG + chr5_2:27706957-27706976 None:intergenic 50.0%
CGTTGTTAGTGACAGGAGTG+AGG + chr5_2:27706990-27707009 None:intergenic 50.0%
TGGACACCGTTGTTAGTGAC+AGG + chr5_2:27706997-27707016 None:intergenic 50.0%
CCTTAGGAGATTGAGGTTCG+TGG + chr5_2:27707017-27707036 None:intergenic 50.0%
CCACGAACCTCAATCTCCTA+AGG - chr5_2:27707014-27707033 Msa0766230:CDS 50.0%
TGGGGTGAGAGTCTTGGTAT+AGG + chr5_2:27707076-27707095 None:intergenic 50.0%
ATCTGATGGGGTGAGAGTCT+TGG + chr5_2:27707082-27707101 None:intergenic 50.0%
CCGCCATTGTTAGCATCTGA+TGG + chr5_2:27707096-27707115 None:intergenic 50.0%
! CAGTATTGAGGTCAAGCGGT+GGG + chr5_2:27707158-27707177 None:intergenic 50.0%
! TCAGTATTGAGGTCAAGCGG+TGG + chr5_2:27707159-27707178 None:intergenic 50.0%
GTTCGCCGACAGACAAAGTT+TGG - chr5_2:27707369-27707388 Msa0766230:intron 50.0%
CGTTGATGCCAAAGTTGTGG+AGG - chr5_2:27707506-27707525 Msa0766230:intron 50.0%
CTGCTCCTAATTGTCCATGG+CGG - chr5_2:27707637-27707656 Msa0766230:intron 50.0%
AGCATCCGCCATGGACAATT+AGG + chr5_2:27707645-27707664 None:intergenic 50.0%
TATACCTCAAGCATCCGCCA+TGG + chr5_2:27707654-27707673 None:intergenic 50.0%
TGTGGAAAAAAGACGGTGCC+TGG + chr5_2:27708533-27708552 None:intergenic 50.0%
TCAAGTGAGCCAAACACTGC+TGG - chr5_2:27708631-27708650 Msa0766230:CDS 50.0%
!! GATTTCTGGCTCAACACGGC+TGG + chr5_2:27706755-27706774 None:intergenic 55.0%
GCCGTGTTGAGCCAGAAATC+TGG - chr5_2:27706755-27706774 Msa0766230:CDS 55.0%
GCACAGCACTGCCAGATTTC+TGG + chr5_2:27706769-27706788 None:intergenic 55.0%
GAAATCTGGCAGTGCTGTGC+AGG - chr5_2:27706769-27706788 Msa0766230:CDS 55.0%
TGGACGGGAACGGTCAAGAA+GGG + chr5_2:27706893-27706912 None:intergenic 55.0%
ATGGACGGGAACGGTCAAGA+AGG + chr5_2:27706894-27706913 None:intergenic 55.0%
CATTCTCTGCACAGTCTCCG+CGG - chr5_2:27706915-27706934 Msa0766230:CDS 55.0%
TTTCGCGCATTCCACTGGCA+CGG + chr5_2:27707127-27707146 None:intergenic 55.0%
AAGTTTCGCCACGTCTACCG+TGG - chr5_2:27707228-27707247 Msa0766230:CDS 55.0%
AGTTTCGCCACGTCTACCGT+GGG - chr5_2:27707229-27707248 Msa0766230:CDS 55.0%
!! TTGTCCATGGCGGATGCTTG+AGG - chr5_2:27707647-27707666 Msa0766230:intron 55.0%
TTCCAGAGCGAGTCAGTCCT+TGG - chr5_2:27708269-27708288 Msa0766230:CDS 55.0%
TCCAGAGCGAGTCAGTCCTT+GGG - chr5_2:27708270-27708289 Msa0766230:CDS 55.0%
TTGATTGCATGCAGGGAGCC+AGG - chr5_2:27708512-27708531 Msa0766230:CDS 55.0%
!! GATTTCTGGCTCAACACGGC+TGG + chr5_2:27706755-27706774 None:intergenic 55.0%
GCCGTGTTGAGCCAGAAATC+TGG - chr5_2:27706755-27706774 Msa0766230:CDS 55.0%
GCACAGCACTGCCAGATTTC+TGG + chr5_2:27706769-27706788 None:intergenic 55.0%
GAAATCTGGCAGTGCTGTGC+AGG - chr5_2:27706769-27706788 Msa0766230:CDS 55.0%
TGGACGGGAACGGTCAAGAA+GGG + chr5_2:27706893-27706912 None:intergenic 55.0%
ATGGACGGGAACGGTCAAGA+AGG + chr5_2:27706894-27706913 None:intergenic 55.0%
CATTCTCTGCACAGTCTCCG+CGG - chr5_2:27706915-27706934 Msa0766230:CDS 55.0%
TTTCGCGCATTCCACTGGCA+CGG + chr5_2:27707127-27707146 None:intergenic 55.0%
AAGTTTCGCCACGTCTACCG+TGG - chr5_2:27707228-27707247 Msa0766230:CDS 55.0%
AGTTTCGCCACGTCTACCGT+GGG - chr5_2:27707229-27707248 Msa0766230:CDS 55.0%
!! TTGTCCATGGCGGATGCTTG+AGG - chr5_2:27707647-27707666 Msa0766230:intron 55.0%
TTCCAGAGCGAGTCAGTCCT+TGG - chr5_2:27708269-27708288 Msa0766230:CDS 55.0%
TCCAGAGCGAGTCAGTCCTT+GGG - chr5_2:27708270-27708289 Msa0766230:CDS 55.0%
TTGATTGCATGCAGGGAGCC+AGG - chr5_2:27708512-27708531 Msa0766230:CDS 55.0%
GATGTCGCAAGGGAGTCCCA+CGG + chr5_2:27707248-27707267 None:intergenic 60.0%
!! TCCCAAGGACTGACTCGCTC+TGG + chr5_2:27708274-27708293 None:intergenic 60.0%
AGAGCGAGTCAGTCCTTGGG+AGG - chr5_2:27708273-27708292 Msa0766230:CDS 60.0%
GATGTCGCAAGGGAGTCCCA+CGG + chr5_2:27707248-27707267 None:intergenic 60.0%
!! TCCCAAGGACTGACTCGCTC+TGG + chr5_2:27708274-27708293 None:intergenic 60.0%
AGAGCGAGTCAGTCCTTGGG+AGG - chr5_2:27708273-27708292 Msa0766230:CDS 60.0%
CGGTGCATTCTCCGTGCCAG+TGG - chr5_2:27707113-27707132 Msa0766230:CDS 65.0%
AGGGAGTCCCACGGTAGACG+TGG + chr5_2:27707239-27707258 None:intergenic 65.0%
CGGTGCATTCTCCGTGCCAG+TGG - chr5_2:27707113-27707132 Msa0766230:CDS 65.0%
AGGGAGTCCCACGGTAGACG+TGG + chr5_2:27707239-27707258 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 27706736 27708861 27706736 ID=Msa0766230;Name=Msa0766230
chr5_2 mRNA 27706736 27708861 27706736 ID=Msa0766230-mRNA-1;Parent=Msa0766230;Name=Msa0766230-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|518
chr5_2 exon 27707929 27708861 27707929 ID=Msa0766230-mRNA-1:exon:10821;Parent=Msa0766230-mRNA-1
chr5_2 exon 27706736 27707359 27706736 ID=Msa0766230-mRNA-1:exon:10820;Parent=Msa0766230-mRNA-1
chr5_2 CDS 27707929 27708861 27707929 ID=Msa0766230-mRNA-1:cds;Parent=Msa0766230-mRNA-1
chr5_2 CDS 27706736 27707359 27706736 ID=Msa0766230-mRNA-1:cds;Parent=Msa0766230-mRNA-1
Gene Sequence

>Msa0766230

ATGTCTTCTCAGCAACCCAGCCGTGTTGAGCCAGAAATCTGGCAGTGCTGTGCAGGCAGTTCCGTTAAAATTCCAAAACTATATTCTCATGTTTACTACTTCCCATTAGGGCACATAGAACACATATGTCCATCCCCAAACCCTAACACACTCTCCCTTCTTGACCGTTCCCGTCCATTCATTCTCTGCACAGTCTCCGCGGTTGATTTACTCGCTGACCTTCACACCGATGAAGTCTTCGTCAAACTACTCCTCACTCCTGTCACTAACAACGGTGTCCACGAACCTCAATCTCCTAAGGTTCGTGAAGACCAAGATGATGGCAAAAAGGTTGTTTCCTATACCAAGACTCTCACCCCATCAGATGCTAACAATGGCGGTGCATTCTCCGTGCCAGTGGAATGCGCGAAATTAATCTTCCCACCGCTTGACCTCAATACTGAAAAGCCGTCTCAAGAAATCTCCATCAGTGACATTCACGGTAAAGTTTGGAAGTTTCGCCACGTCTACCGTGGGACTCCCTTGCGACATCTTCTTACCACTGATTGGAGTGAGTTCGTTGACAAGAAGAAACTAGTTGGCGGCGATTCCTTAGTTTTCATGAAAGACTCAGATGGAAATATCTCAGTCGGAGTTCGCCGACAGACAAAGTTTGGTGCTGCTGCTAAGATAACGGAAAAATTGTTTATGGAGACAGTGGAATTGGCGGATAAAAACATGGCTTTTGAAGTTGTGTATTATCCGACGGCTAAAGGTTGGTGTAATTTTGTCGTTGATGCCAAAGTTGTGGAGGATGCAATGAATATTAAGTGGAATCTTGGAGGGAGAATTGAACTTTCTTTGAAGAATTACGATTCTTCAAAGAGATGCTCAAAATTTGAGGGAACAATATCTGCTCTATCTGCTCCTAATTGTCCATGGCGGATGCTTGAGGTTAAATGGGATGAACCTAAAGTCTCACAGGTTCCAGAGCGAGTCAGTCCTTGGGAGGTTGAAACTATTTCCGACATATTTGCACTGTATCCACAATTCCACCCGACAAAAAAGCTTAGAAAATCTGATCAGGAATCTGCTGCATTCAGTGATAAAAAGGGAGACTCCTTCGTCCCAAATATAGAGGCTTTTTTGAAGATGGTCCCAAATATAGAGTTTAAACATTTCGATATGACATCCTCCAATCAAACATTGTTGAACAATGATGCTCTTCTTGATTGCATGCAGGGAGCCAGGCACCGTCTTTTTTCCACATCAACTTCTTCCAACTTTATAAATGATAAAAGCAATGGTTTCCTTGGGAATAATTATATGGCAGTGTCGAGTAATGTGTCAAGTGAGCCAAACACTGCTGGTCCTATATCTTTGGACTTGTCACCTCATAGCCATGATATTTCTAACTCTCTTGGGACAAAGTTTGCCGGTACTGACAACAGCAATACGAAGAAAGTTAGTCCTGGTTCATTTATGCTATTTGGTAAGATCATAAAACCTGCTGGAAGCGATTTTCAAGAATTAGATATCATGGGAAATGATGATTGTAAAGGCTCCAATGAAATTTAA
Protein sequence

>Msa0766230

MSSQQPSRVEPEIWQCCAGSSVKIPKLYSHVYYFPLGHIEHICPSPNPNTLSLLDRSRPFILCTVSAVDLLADLHTDEVFVKLLLTPVTNNGVHEPQSPKVREDQDDGKKVVSYTKTLTPSDANNGGAFSVPVECAKLIFPPLDLNTEKPSQEISISDIHGKVWKFRHVYRGTPLRHLLTTDWSEFVDKKKLVGGDSLVFMKDSDGNISVGVRRQTKFGAAAKITEKLFMETVELADKNMAFEVVYYPTAKGWCNFVVDAKVVEDAMNIKWNLGGRIELSLKNYDSSKRCSKFEGTISALSAPNCPWRMLEVKWDEPKVSQVPERVSPWEVETISDIFALYPQFHPTKKLRKSDQESAAFSDKKGDSFVPNIEAFLKMVPNIEFKHFDMTSSNQTLLNNDALLDCMQGARHRLFSTSTSSNFINDKSNGFLGNNYMAVSSNVSSEPNTAGPISLDLSPHSHDISNSLGTKFAGTDNSNTKKVSPGSFMLFGKIIKPAGSDFQELDIMGNDDCKGSNEI*