AlfalfaGEDB | Msa0766620

M. sativa cultivar zhongmu-4 / Msa0766620

KEGG: K09422
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766620	sp\|Q9LXF1\|MYB16_ARATH	44.444	297	141	8	9	286	10	301	1.24e-71	226

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766620	G7JWG1	91.753	291	21	3	5	294	1	289	1.27e-171	486

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TFs/TRs:

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Gene ID	Type	Classification
Msa0766620	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766620	MtrunA17_Chr5g0417821	91.753	291	21	3	5	294	1	289	2.44e-175	486
Msa0766620	MtrunA17_Chr4g0042671	41.018	334	118	7	9	265	10	341	1.05e-73	231
Msa0766620	MtrunA17_Chr6g0453471	63.871	155	50	1	9	157	10	164	9.14e-72	225
Msa0766620	MtrunA17_Chr7g0217741	61.963	163	57	2	9	167	10	171	7.64e-71	221
Msa0766620	MtrunA17_Chr8g0349261	59.146	164	58	3	1	159	7	166	1.69e-63	202
Msa0766620	MtrunA17_Chr2g0281071	70.588	119	35	0	10	128	12	130	5.84e-61	197
Msa0766620	MtrunA17_Chr4g0048511	69.748	119	36	0	10	128	12	130	3.88e-60	195
Msa0766620	MtrunA17_Chr5g0410961	66.935	124	41	0	9	132	10	133	1.67e-59	193
Msa0766620	MtrunA17_Chr3g0115821	66.116	121	41	0	10	130	11	131	3.92e-59	192
Msa0766620	MtrunA17_Chr4g0039971	65.546	119	41	0	10	128	12	130	3.79e-57	187

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766620	AT5G15310.1	44.444	297	141	8	9	286	10	301	1.26e-72	226
Msa0766620	AT5G15310.2	66.667	153	47	2	9	157	10	162	1.70e-71	223
Msa0766620	AT5G15310.4	66.667	153	47	2	9	157	10	162	2.39e-71	221
Msa0766620	AT3G01140.2	65.132	152	49	1	10	157	11	162	7.35e-71	223
Msa0766620	AT3G01140.1	65.132	152	49	1	10	157	54	205	2.36e-70	223
Msa0766620	AT3G61250.1	57.516	153	63	1	9	159	10	162	5.50e-63	201
Msa0766620	AT4G05100.1	70.588	119	35	0	10	128	12	130	1.95e-61	197
Msa0766620	AT3G02940.2	67.742	124	40	0	10	133	11	134	2.49e-61	197
Msa0766620	AT3G02940.1	67.742	124	40	0	10	133	11	134	3.78e-61	197
Msa0766620	AT3G02940.3	67.742	124	40	0	10	133	11	134	3.78e-61	197

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Find 55 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTAACAACAACAACA+AGG	0.689545	5_2:+28529644	Msa0766620:CDS
AAGAAAAGACTTATCAGAAT+GGG	0.406171	5_2:+28529597	Msa0766620:CDS
AATGAGTTGAAGTTCGGAGA+AGG	0.564111	5_2:+28529918	Msa0766620:CDS
ACGAGGAAACTTCAGCTCAG+AGG	0.673756	5_2:+28528423	Msa0766620:CDS
AGAAAAGACTTATCAGAATG+GGG	0.641194	5_2:+28529598	Msa0766620:CDS
AGGGCTCTAGGGTTGAAGAA+AGG	0.597288	5_2:+28528158	Msa0766620:CDS
AGGTGATGATGATCTAATGG+TGG	0.729997	5_2:+28530022	Msa0766620:CDS
ATAAGCTTCTTGTCTTCTTT+TGG	0.163262	5_2:-28528191	None:intergenic
ATAATGAAATAAAGAATCAT+TGG	0.440805	5_2:+28529562	Msa0766620:CDS
ATACATTGCATTGTTGTGTT+TGG	0.412941	5_2:-28529805	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAGCATATGTTACTCAACA+TGG	+	chr5_2:28528212-28528231	Msa0766620:CDS	30.0%
TATATAGGTCTTAGAAGATG+TGG	+	chr5_2:28528352-28528371	Msa0766620:intron	30.0%
TGATACAATGATACTAACAC+GGG	+	chr5_2:28528866-28528885	Msa0766620:intron	30.0%
ACTTACACGCAAATTAAAGT+AGG	+	chr5_2:28529089-28529108	Msa0766620:intron	30.0%
CTTACACGCAAATTAAAGTA+GGG	+	chr5_2:28529090-28529109	Msa0766620:intron	30.0%
GAACATATAATGTCAGTATC+TGG	-	chr5_2:28529162-28529181	None:intergenic	30.0%
TGCATAATATATGTAAGGTC+CGG	+	chr5_2:28529356-28529375	Msa0766620:intron	30.0%
ATTTCATTATCTGTTCTCCT+TGG	-	chr5_2:28529552-28529571	None:intergenic	30.0%
CAAGAAAAGACTTATCAGAA+TGG	+	chr5_2:28529596-28529615	Msa0766620:CDS	30.0%
AGAAAAGACTTATCAGAATG+GGG	+	chr5_2:28529598-28529617	Msa0766620:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	28528140	28530148	28528140	ID=Msa0766620;Name=Msa0766620
chr5_2	mRNA	28528140	28530148	28528140	ID=Msa0766620-mRNA-1;Parent=Msa0766620;Name=Msa0766620-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|1\|1\|3\|0\|294
chr5_2	exon	28528140	28528269	28528140	ID=Msa0766620-mRNA-1:exon:10982;Parent=Msa0766620-mRNA-1
chr5_2	exon	28528359	28528488	28528359	ID=Msa0766620-mRNA-1:exon:10983;Parent=Msa0766620-mRNA-1
chr5_2	exon	28529524	28530148	28529524	ID=Msa0766620-mRNA-1:exon:10984;Parent=Msa0766620-mRNA-1
chr5_2	CDS	28528140	28528269	28528140	ID=Msa0766620-mRNA-1:cds;Parent=Msa0766620-mRNA-1
chr5_2	CDS	28528359	28528488	28528359	ID=Msa0766620-mRNA-1:cds;Parent=Msa0766620-mRNA-1
chr5_2	CDS	28529524	28530148	28529524	ID=Msa0766620-mRNA-1:cds;Parent=Msa0766620-mRNA-1

Gene Sequence

>Msa0766620

TGTGATTTGAAAATGAGTAGGGCTCTAGGGTTGAAGAAAGGACCATGGTCCCCAAAAGAAGACAAGAAGCTTATAGCATATGTTACTCAACATGGCCATAGAAACTGGCATTCTTTGCCCTCCAGAGCAGGTCTTAGAAGATGTGGAAAGAGTTGCAGACTGAGGTGGATTAACTACCTGAATCCTGATATAAAACGAGGAAACTTCAGCTCAGAGGAAGATCACACTATTATTCAGCTTCATGCACTTCTCGGGAACAAATGGTCCACAATAGCAGCTCTCCTGCCAAGGAGAACAGATAATGAAATAAAGAATCATTGGAACAGTAACATCAAGAAAAGACTTATCAGAATGGGGATAGATCCCACCACTCACAAACCAAAATCTAACAACAACAACAAGGACAATGTCAATACTGCTATCAAACACGTGGCTCAATGGGAGAATGTTAGATTAGAAGCTGAAGCAAGATCATCAATGTTAGGAAAAACTCAAACTCAACCTTTACTATCTTCATCAAGTTCATCAAAGTTATCACTAACCAAACACAACAATGCAATGTATAACTTGTGTGCCATCATGCTTGCAAATAATGATGACTCTTTTTCTTCAACATCCACATTGAGCTGTCCTAATGAGCTTCCTTGTCTTACCAATGAGTTGAAGTTCGGAGAAGGCTTAGGATCATATGAGAGTGTTAGTAATAATAATATCATGGAAACTATTGCTCAAGATGATGTTTATGTGTCTAAATTGCAAGGTGATGATGATCTAATGGTGGCATTGGATGCATTTGGAGATTCCTTCAATGGTACTGACACTTTCATAGACTTCAATGGAAATGGAATGTGCAATTTTGATGCTAGCTTGGCATTTTTTGAGTAA

Protein sequence

>Msa0766620

CDLKMSRALGLKKGPWSPKEDKKLIAYVTQHGHRNWHSLPSRAGLRRCGKSCRLRWINYLNPDIKRGNFSSEEDHTIIQLHALLGNKWSTIAALLPRRTDNEIKNHWNSNIKKRLIRMGIDPTTHKPKSNNNNKDNVNTAIKHVAQWENVRLEAEARSSMLGKTQTQPLLSSSSSSKLSLTKHNNAMYNLCAIMLANNDDSFSSTSTLSCPNELPCLTNELKFGEGLGSYESVSNNNIMETIAQDDVYVSKLQGDDDLMVALDAFGDSFNGTDTFIDFNGNGMCNFDASLAFFE*