AlfalfaGEDB | Msa0767640

M. sativa cultivar zhongmu-4 / Msa0767640

KEGG: K14846
GO: GO:0000027 GO:0000054 GO:0000055 GO:0000460 GO:0000463 GO:0000466 GO:0000470 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016072 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0031503 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0033750 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034660 GO:0042134 GO:0042254 GO:0042255 GO:0042273 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050657 GO:0050658 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0065003 GO:0070013 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0767640	A0A072VU07	47.869	305	131	5	1	280	4	305	4.74e-95	291

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TFs/TRs:

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Gene ID	Type	Classification
Msa0767640	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0767640	MtrunA17_Chr6g0473661	35.294	238	140	4	54	277	256	493	3.60e-43	154
Msa0767640	MtrunA17_Chr7g0232921	35.808	229	89	6	1	202	4	201	7.53e-38	132
Msa0767640	MtrunA17_Chr4g0023021	50.000	110	55	0	171	280	8	117	5.46e-33	117
Msa0767640	MtrunA17_Chr8g0366201	48.246	114	58	1	167	280	2	114	4.20e-32	114
Msa0767640	MtrunA17_Chr1g0206471	29.046	241	146	3	30	245	28	268	6.15e-29	111
Msa0767640	MtrunA17_Chr4g0054601	70.769	65	19	0	67	131	1	65	2.16e-27	102
Msa0767640	MtrunA17_Chr1g0173661	47.368	95	50	0	186	280	1	95	2.53e-27	101
Msa0767640	MtrunA17_Chr7g0270401	27.313	227	140	3	40	241	37	263	2.10e-23	96.3
Msa0767640	MtrunA17_Chr6g0473921	32.061	131	88	1	148	277	2	132	1.13e-21	88.6
Msa0767640	MtrunA17_Chr6g0473651	37.879	132	69	3	54	172	225	356	2.98e-20	89.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 66 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGACAAAGTCGTTCA+TGG	0.370649	5_2:+31215376	None:intergenic
ACAAAGTCGGATGTTACCTC+TGG	0.536950	5_2:-31214464	Msa0767640:CDS
ACAAAGTTGAGCAATACTTT+TGG	0.346062	5_2:-31214048	Msa0767640:CDS
ACAATGTTGGTGGTGGTCTT+GGG	0.369416	5_2:-31215181	Msa0767640:CDS
ACGACTTTGTCAAGTTTGGC+AGG	0.473314	5_2:-31215370	Msa0767640:CDS
ACTTTACATGCGTAATTTCT+GGG	0.435509	5_2:-31214240	Msa0767640:CDS
ATAACATCCGTGCTTGCTCT+TGG	0.387270	5_2:-31214697	Msa0767640:CDS
ATATCTCACCAGCGAACCAG+AGG	0.646756	5_2:+31214448	None:intergenic
ATCCAACATCGTACTCCATA+CGG	0.582851	5_2:+31215120	None:intergenic
ATCGACCATAGTCAAACATG+TGG	0.624542	5_2:+31214312	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAGATTATGTGCTTCAAAA+TGG	-	chr5_2:31214447-31214466	Msa0767640:intron	30.0%
TCCATGTGAAAATGAAATTG+AGG	-	chr5_2:31214627-31214646	Msa0767640:intron	30.0%
GTTAAGATTGTGAAGAAGAA+TGG	-	chr5_2:31214649-31214668	Msa0767640:CDS	30.0%
TGGAAATGCAATATGAAGTT+CGG	-	chr5_2:31215139-31215158	Msa0767640:CDS	30.0%
ACTTTACATGCGTAATTTCT+GGG	-	chr5_2:31215173-31215192	Msa0767640:CDS	30.0%
CTTACACGTAAGTTTAATGA+AGG	-	chr5_2:31215214-31215233	Msa0767640:intron	30.0%
TATTGCTCAACTTTGTAAAC+AGG	+	chr5_2:31215361-31215380	None:intergenic	30.0%
ACAGATTATGTGCTTCAAAA+TGG	-	chr5_2:31214447-31214466	Msa0767640:intron	30.0%
TCCATGTGAAAATGAAATTG+AGG	-	chr5_2:31214627-31214646	Msa0767640:intron	30.0%
GTTAAGATTGTGAAGAAGAA+TGG	-	chr5_2:31214649-31214668	Msa0767640:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	31214039	31215396	31214039	ID=Msa0767640;Name=Msa0767640
chr5_2	mRNA	31214039	31215396	31214039	ID=Msa0767640-mRNA-1;Parent=Msa0767640;Name=Msa0767640-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0\|0\|0\|0.6\|1\|1\|5\|0\|280
chr5_2	exon	31215333	31215396	31215333	ID=Msa0767640-mRNA-1:exon:11592;Parent=Msa0767640-mRNA-1
chr5_2	exon	31215118	31215209	31215118	ID=Msa0767640-mRNA-1:exon:11591;Parent=Msa0767640-mRNA-1
chr5_2	exon	31214641	31214882	31214641	ID=Msa0767640-mRNA-1:exon:11590;Parent=Msa0767640-mRNA-1
chr5_2	exon	31214457	31214559	31214457	ID=Msa0767640-mRNA-1:exon:11589;Parent=Msa0767640-mRNA-1
chr5_2	exon	31214039	31214380	31214039	ID=Msa0767640-mRNA-1:exon:11588;Parent=Msa0767640-mRNA-1
chr5_2	CDS	31215333	31215396	31215333	ID=Msa0767640-mRNA-1:cds;Parent=Msa0767640-mRNA-1
chr5_2	CDS	31215118	31215209	31215118	ID=Msa0767640-mRNA-1:cds;Parent=Msa0767640-mRNA-1
chr5_2	CDS	31214641	31214882	31214641	ID=Msa0767640-mRNA-1:cds;Parent=Msa0767640-mRNA-1
chr5_2	CDS	31214457	31214559	31214457	ID=Msa0767640-mRNA-1:cds;Parent=Msa0767640-mRNA-1
chr5_2	CDS	31214039	31214380	31214039	ID=Msa0767640-mRNA-1:cds;Parent=Msa0767640-mRNA-1

Gene Sequence

>Msa0767640

ATGAACGACTTTGTCAAGTTTGGCAGGGATCAAGAAGCCATTGAAGCACTGATGAGTTTAAAAGTCCAGGACAATGTTGGTGGTGGTCTTGGGAGGAAGGTTCGTTCAGACTTGATTAAGGTGAGGACATACCGTATGGAGTACGATGTTGGATCTCGCAAGGCAAAGCTACCAGCATGTTTTTCAAGGGAATATGGAATGGAGGTTGCTCCATTCGTTTTCCTACGTGATCCATGTGAAAATGAAATTGAGGTTAAGATTGTGAAGAAGAATGGAAGAGTTTACTTTGATGATGGTTGGTCTAAACTTAAGGATCTTTATAACATCCGTGCTTGCTCTTGGATCACAATCATTTATGCGAAACGCAACTTGTTTCTTTTTCGTGGAGAAACTATGAGCAACATTCCTAATCATCATTATGATGCTCGCTTTCTGCCAACGAAGTTTTACCACACCTATGTGAAGGAACTGACAAAGTCGGATGTTACCTCTGGTTCGCTGGAGATGATGTGGCTTGGCTTCTGTCAATCTGCTTTGCCTGCTGAACCCACATGTTTGACTATGGTCGATTACTTGGGATATTCATGGAAATGCAATATGAAGTTCGGTAATGGGAAGGACTTTACATGCGTAATTTCTGGGCAGTGGAAGCATATTTGTCTTACACGTAAGTTTAATGAAGGTGATATGGTGAAGTTTGGTATGACTAAGGCTGCAAACAACACGATTGTTCACATTCTCTCGCCTTCAATGACGGTTCTCCGAACAATGCTGCCTCTGTCCCGCGTTGCTGGTGCAACTGGGCCTGTTTACAAAGTTGAGCAATACTTTTGGAAAAACTAA

Protein sequence

>Msa0767640

MNDFVKFGRDQEAIEALMSLKVQDNVGGGLGRKVRSDLIKVRTYRMEYDVGSRKAKLPACFSREYGMEVAPFVFLRDPCENEIEVKIVKKNGRVYFDDGWSKLKDLYNIRACSWITIIYAKRNLFLFRGETMSNIPNHHYDARFLPTKFYHTYVKELTKSDVTSGSLEMMWLGFCQSALPAEPTCLTMVDYLGYSWKCNMKFGNGKDFTCVISGQWKHICLTRKFNEGDMVKFGMTKAANNTIVHILSPSMTVLRTMLPLSRVAGATGPVYKVEQYFWKN*