AlfalfaGEDB | Msa0768090

M. sativa cultivar zhongmu-4 / Msa0768090

KEGG: K14846
GO: GO:0000027 GO:0000054 GO:0000055 GO:0000460 GO:0000463 GO:0000466 GO:0000470 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016072 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0031503 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0033750 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034660 GO:0042134 GO:0042254 GO:0042255 GO:0042273 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050657 GO:0050658 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0065003 GO:0070013 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0768090	A0A072VU07	46.816	267	112	4	1	244	46	305	4.78e-78	247

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TFs/TRs:

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Gene ID	Type	Classification
Msa0768090	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0768090	MtrunA17_Chr6g0473661	32.922	243	148	4	9	241	256	493	7.18e-39	140
Msa0768090	MtrunA17_Chr4g0023021	48.246	114	59	0	131	244	4	117	5.86e-34	118
Msa0768090	MtrunA17_Chr8g0366201	48.182	110	56	1	135	244	6	114	1.33e-31	112
Msa0768090	MtrunA17_Chr4g0054601	53.535	99	27	1	22	101	1	99	1.16e-27	102
Msa0768090	MtrunA17_Chr1g0173661	47.368	95	50	0	150	244	1	95	2.32e-27	100
Msa0768090	MtrunA17_Chr7g0232921	35.870	184	69	5	1	166	49	201	4.08e-25	98.2
Msa0768090	MtrunA17_Chr1g0206471	29.204	226	142	4	1	209	44	268	2.03e-24	98.2
Msa0768090	MtrunA17_Chr6g0473921	31.298	131	89	1	112	241	2	132	3.27e-21	86.3
Msa0768090	MtrunA17_Chr8g0365871	48.000	75	39	0	131	205	13	87	4.88e-20	82.0
Msa0768090	MtrunA17_Chr4g0054611	59.091	66	26	1	164	229	1	65	3.66e-19	79.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 61 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAAGTCGGATGTTACCTC+TGG	0.536950	5_2:+31967590	Msa0768090:CDS
ACAATGTTGGTGGTGGTCTT+GGG	0.369416	5_2:+31966873	Msa0768090:five_prime_UTR
ACTTTACATGCATAATTTCT+GGG	0.453100	5_2:+31967814	Msa0768090:CDS
ATAAAGTTGAGCAATACTTT+TGG	0.349814	5_2:+31968006	Msa0768090:CDS
ATAACATCCGTGCTTGCTCT+TGG	0.387270	5_2:+31967357	Msa0768090:CDS
ATATCTCACCTGCGAACCAG+AGG	0.633490	5_2:-31967606	None:intergenic
ATCCAACATCGTACTCCATA+CGG	0.582851	5_2:-31966934	None:intergenic
ATCGACCATAGTCAAACATG+TGG	0.624542	5_2:-31967742	None:intergenic
ATGTTGGTGGTGGTCTTGGG+AGG	0.618537	5_2:+31966876	Msa0768090:five_prime_UTR
CAAGCAGTCCAGGACAATGT+TGG	0.603347	5_2:+31966860	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAGATTATGTGCTTCAAAA+TGG	+	chr5_2:31967088-31967107	Msa0768090:intron	30.0%
TCCATGTGAAAATGAAATTG+AGG	+	chr5_2:31967268-31967287	Msa0768090:CDS	30.0%
CAAGCATCATAATGATGATT+AGG	-	chr5_2:31967527-31967546	None:intergenic	30.0%
TGGAAATGCAATATGAAGTT+CGG	+	chr5_2:31967780-31967799	Msa0768090:CDS	30.0%
GACTTTACATGCATAATTTC+TGG	+	chr5_2:31967813-31967832	Msa0768090:CDS	30.0%
CTTACACGTAAGTTTAATGA+AGG	+	chr5_2:31967855-31967874	Msa0768090:CDS	30.0%
ACAGATTATGTGCTTCAAAA+TGG	+	chr5_2:31967088-31967107	Msa0768090:intron	30.0%
TCCATGTGAAAATGAAATTG+AGG	+	chr5_2:31967268-31967287	Msa0768090:CDS	30.0%
CAAGCATCATAATGATGATT+AGG	-	chr5_2:31967527-31967546	None:intergenic	30.0%
TGGAAATGCAATATGAAGTT+CGG	+	chr5_2:31967780-31967799	Msa0768090:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	31966867	31968034	31966867	ID=Msa0768090;Name=Msa0768090
chr5_2	mRNA	31966867	31968034	31966867	ID=Msa0768090-mRNA-1;Parent=Msa0768090;Name=Msa0768090-mRNA-1;_AED=0.10;_eAED=0.10;_QI=71\|0\|0\|0.75\|1\|0.75\|4\|0\|244
chr5_2	exon	31966867	31966958	31966867	ID=Msa0768090-mRNA-1:exon:11694;Parent=Msa0768090-mRNA-1
chr5_2	exon	31967194	31967435	31967194	ID=Msa0768090-mRNA-1:exon:11695;Parent=Msa0768090-mRNA-1
chr5_2	exon	31967490	31967619	31967490	ID=Msa0768090-mRNA-1:exon:11696;Parent=Msa0768090-mRNA-1
chr5_2	exon	31967696	31968034	31967696	ID=Msa0768090-mRNA-1:exon:11697;Parent=Msa0768090-mRNA-1
chr5_2	five_prime_UTR	31966867	31966937	31966867	ID=Msa0768090-mRNA-1:five_prime_utr;Parent=Msa0768090-mRNA-1
chr5_2	CDS	31966938	31966958	31966938	ID=Msa0768090-mRNA-1:cds;Parent=Msa0768090-mRNA-1
chr5_2	CDS	31967194	31967435	31967194	ID=Msa0768090-mRNA-1:cds;Parent=Msa0768090-mRNA-1
chr5_2	CDS	31967490	31967619	31967490	ID=Msa0768090-mRNA-1:cds;Parent=Msa0768090-mRNA-1
chr5_2	CDS	31967696	31968034	31967696	ID=Msa0768090-mRNA-1:cds;Parent=Msa0768090-mRNA-1

Gene Sequence

>Msa0768090

ATGGAGTACGATGTTGGATCTCGCAAGGCAAAGCTACTAGCATGTTTTTCAAGGGAATATGGAATGGAGGTTGCTCCATTCGTTTTCCTACGTGATCCATGTGAAAATGAAATTGAGGTTAAAATTGTGAAGAAGAATGGAAGAGTTTACTTTGATGATGGTTGGTCTAAACCTAAGGATCTTTATAACATCCGTGCTTGCTCTTGGATCACAATCATTTATGCGAAACGCAACTTGTTTCTTTTTCGTGGAGAAACTATGAGACGCACACCAAGCGTTGCAGTGAATAGCAACATTCCTAATCATCATTATGATGCTTGCTTTCTGCCAACGAAGTTTTACCACACCTATGTGAAGGAACTGACAAAGTCGGATGTTACCTCTGGTTCGCAGGAGATGATGTGGCTTGGCTTCTGTCAATCTGCTTTACCTGCTGAACCCACATGTTTGACTATGGTCGATTACTTGGGATATTCATGGAAATGCAATATGAAGTTCGGTAATGGGAAGGACTTTACATGCATAATTTCTGGGCAGTGGAAGCATATTTGTCTTACACGTAAGTTTAATGAAGGTGATATGGTGAAGTTTGGTATGACTAAGGCTGCAAACAACACGATTGTTCACATTCTCTCGCCTTCAATGACGGTTCTTCGAACAATGCTGCCTCTGTCCCGCGTTGCTGGTGCAACTGGGCCTGTTTATAAAGTTGAGCAATACTTTTGGAAAAAC

Protein sequence

>Msa0768090

MEYDVGSRKAKLLACFSREYGMEVAPFVFLRDPCENEIEVKIVKKNGRVYFDDGWSKPKDLYNIRACSWITIIYAKRNLFLFRGETMRRTPSVAVNSNIPNHHYDACFLPTKFYHTYVKELTKSDVTSGSQEMMWLGFCQSALPAEPTCLTMVDYLGYSWKCNMKFGNGKDFTCIISGQWKHICLTRKFNEGDMVKFGMTKAANNTIVHILSPSMTVLRTMLPLSRVAGATGPVYKVEQYFWKN