AlfalfaGEDB | Msa0769100

M. sativa cultivar zhongmu-4 / Msa0769100

KEGG: K09414|K09419
GO: GO:0000003 GO:0000302 GO:0003006 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009958 GO:0010033 GO:0010154 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048530 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090696 GO:0090698 GO:0099402 GO:0140110 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0769100	sp\|Q9FK72\|HFA4C_ARATH	51.773	141	67	1	12	152	32	171	1.05e-43	150

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0769100	A0A1S2X9Y7	63.504	137	47	1	1	134	21	157	6.41e-53	182

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TFs/TRs:

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Gene ID	Type	Classification
Msa0769100	TF	HSF

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0769100	MtrunA17_Chr2g0284481	54.167	144	64	1	1	142	23	166	2.07e-49	163
Msa0769100	MtrunA17_Chr8g0379701	57.143	126	53	1	1	126	20	144	2.03e-46	155
Msa0769100	MtrunA17_Chr5g0410881	46.099	141	70	2	1	135	40	180	6.12e-36	130
Msa0769100	MtrunA17_Chr8g0378061	52.308	130	53	3	1	130	30	150	9.44e-36	129
Msa0769100	MtrunA17_Chr4g0039891	44.595	148	61	2	1	135	20	159	3.21e-35	128
Msa0769100	MtrunA17_Chr8g0392001	47.826	138	66	2	1	132	30	167	1.34e-33	121
Msa0769100	MtrunA17_Chr6g0467041	57.778	90	38	0	1	90	30	119	8.51e-33	119
Msa0769100	MtrunA17_Chr4g0071051	43.750	144	73	2	1	144	109	244	2.35e-32	119
Msa0769100	MtrunA17_Chr7g0260331	42.254	142	79	2	1	139	57	198	3.59e-32	117
Msa0769100	MtrunA17_Chr1g0185131	42.520	127	61	1	1	127	40	154	1.00e-30	113

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0769100	AT5G45710.3	51.773	141	67	1	12	152	32	171	1.07e-44	150
Msa0769100	AT5G45710.1	51.773	141	67	1	12	152	32	171	1.07e-44	150
Msa0769100	AT4G18880.1	53.390	118	55	0	18	135	40	157	2.70e-39	137
Msa0769100	AT4G13980.1	50.000	134	62	2	1	134	31	159	2.00e-35	128
Msa0769100	AT2G26150.1	46.512	129	64	2	1	127	52	177	2.05e-34	124
Msa0769100	AT2G26150.4	46.512	129	64	2	1	127	52	177	2.05e-34	124
Msa0769100	AT4G17750.1	43.243	148	71	1	1	135	60	207	4.94e-34	125
Msa0769100	AT1G32330.1	46.154	143	53	2	1	127	45	179	7.84e-34	124
Msa0769100	AT1G32330.2	46.154	143	53	2	1	127	45	179	8.00e-34	124
Msa0769100	AT3G02990.1	43.478	138	75	1	1	135	31	168	2.27e-32	120

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Find 23 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTCACTAACCAAATTCA+AGG	0.460850	5_2:+36594359	Msa0769100:CDS
AATGATGAAACTTCTACAGT+TGG	0.549787	5_2:-36592917	None:intergenic
ACAAGCATGGGCCTTGAATT+TGG	0.207373	5_2:-36594370	None:intergenic
ACGTCTTGTACAACAAGCAT+GGG	0.611089	5_2:-36594382	None:intergenic
ACTTCTACAGTTGGCACTCC+AGG	0.475767	5_2:-36592908	None:intergenic
AGAAGGAGTGTATCTTGTGA+AGG	0.580834	5_2:+36594311	Msa0769100:CDS
AGGAACAAGAGAAGAAAAGA+AGG	0.466768	5_2:+36594294	Msa0769100:CDS
ATCTGATGAAGAATATCCAT+AGG	0.645119	5_2:+36594129	Msa0769100:CDS
CAATTATAGCATTCAAATTC+TGG	0.188343	5_2:-36594223	None:intergenic
CATGTGTATTCTTCAAAGAA+TGG	0.490214	5_2:-36594169	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGATGAAACTTCTACAGT+TGG	-	chr5_2:36592920-36592939	None:intergenic	30.0%
ACAGTAAAAAACCCTTAATG+GGG	-	chr5_2:36593136-36593155	None:intergenic	30.0%
CACAGTAAAAAACCCTTAAT+GGG	-	chr5_2:36593137-36593156	None:intergenic	30.0%
ACACAGTAAAAAACCCTTAA+TGG	-	chr5_2:36593138-36593157	None:intergenic	30.0%
GAATAATGATCGTTATGGAT+TGG	+	chr5_2:36593241-36593260	Msa0769100:intron	30.0%
CTTGAAATATGTTACTCACA+TGG	+	chr5_2:36593490-36593509	Msa0769100:intron	30.0%
TCAAACCTGAAACATTAAGA+GGG	-	chr5_2:36593555-36593574	None:intergenic	30.0%
TGGATATTCTTCATCAGATA+TGG	-	chr5_2:36594128-36594147	None:intergenic	30.0%
ATCTGATGAAGAATATCCAT+AGG	+	chr5_2:36594129-36594148	Msa0769100:CDS	30.0%
CATGTGTATTCTTCAAAGAA+TGG	-	chr5_2:36594172-36594191	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	36592874	36594406	36592874	ID=Msa0769100;Name=Msa0769100
chr5_2	mRNA	36592874	36594406	36592874	ID=Msa0769100-mRNA-1;Parent=Msa0769100;Name=Msa0769100-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0\|0\|1\|1\|1\|2\|0\|166
chr5_2	exon	36592874	36593029	36592874	ID=Msa0769100-mRNA-1:exon:12215;Parent=Msa0769100-mRNA-1
chr5_2	exon	36594062	36594406	36594062	ID=Msa0769100-mRNA-1:exon:12216;Parent=Msa0769100-mRNA-1
chr5_2	CDS	36592874	36593029	36592874	ID=Msa0769100-mRNA-1:cds;Parent=Msa0769100-mRNA-1
chr5_2	CDS	36594062	36594406	36594062	ID=Msa0769100-mRNA-1:cds;Parent=Msa0769100-mRNA-1

Gene Sequence

>Msa0769100

ATGGTTGAAGATTCTTTAACTGATTCCATTATTTCCTGGAGTGCCAACTGTAGAAGTTTCATCATTTCGAATTCATTCGATCTTGCAAAGGATTTGTTACCTCGATATTTTAAGCATAATAACTTCTCAAGTTTCAATAGACGATTGAATACATATGGATTTAGAAAAGTTGATTCAAAAAAATGGGAATTTGCAAACGATAATTTTGTAAAAGGTCAACCATATCTGATGAAGAATATCCATAGGCAACAATCTTTTCGTAGCCATTCTTTGAAGAATACACATGGACAAGGGGATGCTACTCGTGAAATAGAATGCCAGAATTTGAATGCTATAATTGAAAATCTTAAACGTGACAAGGAACATATTCTTCTTGAACTTCAAACAAAGGAACAAGAGAAGAAAAGAAGGAGTGTATCTTGTGAAGGTATAGCTTGTGAATGTTGTCATGTTAAATTCACTAACCAAATTCAAGGCCCATGCTTGTTGTACAAGACGTAG

Protein sequence

>Msa0769100

MVEDSLTDSIISWSANCRSFIISNSFDLAKDLLPRYFKHNNFSSFNRRLNTYGFRKVDSKKWEFANDNFVKGQPYLMKNIHRQQSFRSHSLKNTHGQGDATREIECQNLNAIIENLKRDKEHILLELQTKEQEKKRRSVSCEGIACECCHVKFTNQIQGPCLLYKT*