AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0769960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0769960 MtrunA17_Chr4g0009901 74.144 263 67 1 1 263 1 262 7.40e-139 393
Msa0769960 MtrunA17_Chr4g0009851 61.921 302 112 3 1 300 1 301 1.23e-127 366
Msa0769960 MtrunA17_Chr4g0009981 59.438 249 99 2 53 300 1 248 6.59e-96 284
Msa0769960 MtrunA17_Chr1g0161431 46.586 249 99 4 10 226 11 257 1.35e-65 217
Msa0769960 MtrunA17_Chr4g0011071 64.493 138 48 1 55 192 35 171 4.61e-60 189
Msa0769960 MtrunA17_Chr2g0294911 44.398 241 109 3 10 226 29 268 9.26e-59 198
Msa0769960 MtrunA17_Chr4g0067981 44.672 244 106 5 10 226 31 272 3.04e-57 195
Msa0769960 MtrunA17_Chr5g0423031 92.135 89 7 0 1 89 1 89 8.80e-56 179
Msa0769960 MtrunA17_Chr5g0423031 83.158 95 14 1 185 277 80 174 1.83e-40 139
Msa0769960 MtrunA17_Chr3g0123131 40.408 245 117 5 10 226 13 256 1.65e-52 181
Msa0769960 MtrunA17_Chr6g0471041 43.529 255 111 7 1 237 1 240 3.05e-50 172
Msa0769960 MtrunA17_Chr4g0009991 71.552 116 32 1 103 218 2 116 3.17e-50 162
Msa0769960 MtrunA17_Chr3g0137101 40.964 249 114 5 10 226 8 255 5.34e-50 175
Msa0769960 MtrunA17_Chr8g0376881 37.705 244 125 4 9 226 24 266 1.99e-48 169
Msa0769960 MtrunA17_Chr1g0149841 38.956 249 121 5 7 226 2 248 3.52e-48 168
Msa0769960 MtrunA17_Chr4g0010001 79.775 89 18 0 1 89 1 89 1.09e-46 152
Msa0769960 MtrunA17_Chr8g0391911 39.095 243 122 3 1 218 1 242 1.22e-46 158
Msa0769960 MtrunA17_Chr7g0275401 34.672 274 147 5 7 250 2 273 1.52e-45 156
Msa0769960 MtrunA17_Chr2g0304841 35.686 255 126 5 10 227 18 271 3.01e-45 163
Msa0769960 MtrunA17_Chr4g0074411 37.500 248 129 4 10 232 18 264 1.81e-44 160
Msa0769960 MtrunA17_Chr4g0074401 38.367 245 122 5 10 226 17 260 3.06e-44 159
Msa0769960 MtrunA17_Chr7g0275891 34.866 261 126 7 7 225 2 260 8.11e-43 150
Msa0769960 MtrunA17_Chr8g0383961 41.441 222 102 6 10 230 14 208 3.45e-41 144
Msa0769960 MtrunA17_Chr1g0149851 36.364 264 122 7 7 226 2 263 4.78e-41 149
Msa0769960 MtrunA17_Chr6g0456631 43.478 161 89 2 1 160 1 160 2.79e-39 136
Msa0769960 MtrunA17_Chr3g0134461 35.714 210 109 3 10 218 8 192 1.60e-38 135
Msa0769960 MtrunA17_Chr4g0074421 39.560 182 87 3 59 218 2 182 4.78e-36 128
Msa0769960 MtrunA17_Chr3g0134481 33.962 212 103 3 10 220 8 183 1.88e-34 124
Msa0769960 MtrunA17_Chr1g0149831 35.294 187 88 5 68 226 1 182 3.17e-26 103
Msa0769960 MtrunA17_Chr7g0261911 49.367 79 39 1 160 237 253 331 1.98e-16 79.7
Msa0769960 MtrunA17_Chr5g0417521 60.345 58 22 1 165 221 11 68 5.80e-16 77.4
Msa0769960 MtrunA17_Chr2g0321461 41.748 103 49 3 125 221 1 98 7.68e-16 77.4
Msa0769960 MtrunA17_Chr6g0473731 36.170 141 75 3 101 231 21 156 1.09e-15 77.4
Msa0769960 MtrunA17_Chr4g0031961 50.685 73 35 1 155 226 116 188 1.77e-15 75.9
Msa0769960 MtrunA17_Chr6g0461641 56.061 66 26 2 165 229 38 101 2.18e-15 76.3
Msa0769960 MtrunA17_Chr7g0255711 59.322 59 24 0 168 226 90 148 2.50e-15 75.1
Msa0769960 MtrunA17_Chr7g0274471 51.429 70 33 1 169 237 218 287 7.82e-15 74.7
Msa0769960 MtrunA17_Chr1g0190301 48.193 83 40 2 169 250 175 255 1.35e-14 73.6
Msa0769960 MtrunA17_Chr2g0283951 36.800 125 68 5 154 268 69 192 1.39e-14 73.6
Msa0769960 MtrunA17_Chr3g0095611 49.351 77 32 2 159 228 3 79 2.14e-14 70.9
Msa0769960 MtrunA17_Chr1g0162931 54.386 57 25 1 167 222 16 72 7.97e-14 70.5
Msa0769960 MtrunA17_Chr7g0243081 55.357 56 24 1 167 221 69 124 8.38e-14 71.2
Msa0769960 MtrunA17_Chr4g0042451 55.172 58 25 1 165 221 13 70 3.42e-13 69.3
Msa0769960 MtrunA17_Chr8g0379121 53.571 56 25 1 167 221 109 164 6.92e-13 68.9
Msa0769960 MtrunA17_Chr2g0324891 41.250 80 47 0 146 225 138 217 7.51e-13 68.6
Msa0769960 MtrunA17_Chr8g0356501 51.515 66 31 1 157 221 25 90 7.61e-13 68.2
Msa0769960 MtrunA17_Chr7g0254981 36.765 136 74 4 112 244 2 128 8.34e-13 67.4
Msa0769960 MtrunA17_Chr2g0310011 51.515 66 31 1 157 221 23 88 1.05e-12 67.8
Msa0769960 MtrunA17_Chr1g0210911 52.632 57 26 1 167 222 20 76 1.16e-12 67.8
Msa0769960 MtrunA17_Chr1g0197171 59.259 54 21 1 169 221 44 97 1.29e-12 67.0
Msa0769960 MtrunA17_Chr4g0054981 46.341 82 43 1 164 245 305 385 2.68e-12 67.4
Msa0769960 MtrunA17_Chr7g0258551 53.571 56 25 1 167 221 210 265 3.09e-12 66.6
Msa0769960 MtrunA17_Chr4g0040481 51.724 58 28 0 168 225 157 214 3.85e-12 66.6
Msa0769960 MtrunA17_Chr2g0291021 46.067 89 38 3 169 247 49 137 4.28e-12 65.9
Msa0769960 MtrunA17_Chr2g0288601 53.704 54 24 1 166 218 68 121 4.29e-12 62.4
Msa0769960 MtrunA17_Chr2g0290991 57.407 54 22 1 169 221 39 92 4.44e-12 65.9
Msa0769960 MtrunA17_Chr1g0175091 44.444 81 40 3 160 239 251 327 5.10e-12 66.2
Msa0769960 MtrunA17_Chr2g0320231 50.877 57 27 1 167 222 95 151 5.53e-12 65.9
Msa0769960 MtrunA17_Chr1g0175071 57.895 57 23 1 169 224 177 233 7.75e-12 65.1
Msa0769960 MtrunA17_Chr5g0400241 46.479 71 38 0 158 228 300 370 9.76e-12 65.5
Msa0769960 MtrunA17_Chr5g0400241 29.921 127 83 4 10 134 14 136 4.67e-11 63.5
Msa0769960 MtrunA17_Chr5g0409731 55.556 54 23 1 169 221 25 78 1.19e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0769960 AT3G16857.1 40.684 263 109 4 10 226 33 294 7.00e-56 192
Msa0769960 AT3G16857.2 40.684 263 109 4 10 226 33 294 7.78e-56 192
Msa0769960 AT2G01760.1 41.569 255 113 5 7 226 4 257 1.14e-53 180
Msa0769960 AT1G67710.1 39.623 265 128 6 11 244 8 271 3.87e-50 174
Msa0769960 AT2G01760.2 37.456 283 113 6 7 226 4 285 1.13e-47 165
Msa0769960 AT2G25180.1 39.419 241 121 4 10 226 13 252 1.53e-46 166
Msa0769960 AT4G31920.1 38.889 234 128 5 10 230 13 244 1.14e-43 157
Msa0769960 AT5G58080.1 39.024 246 124 5 10 232 14 256 3.80e-43 157
Msa0769960 AT3G16857.3 39.286 224 89 4 49 226 2 224 1.01e-41 153
Msa0769960 AT3G62670.1 35.714 224 120 7 27 227 52 274 8.54e-34 128
Msa0769960 AT2G01760.3 38.860 193 83 4 68 226 1 192 7.84e-32 121
Msa0769960 AT1G49190.1 32.114 246 129 7 10 221 30 271 1.39e-30 119
Msa0769960 AT5G49240.1 31.646 237 134 5 9 218 37 272 8.30e-30 115
Msa0769960 AT1G67710.2 37.681 207 98 5 68 244 1 206 1.45e-29 117
Msa0769960 AT5G07210.2 27.306 271 140 6 14 230 16 283 6.25e-23 99.4
Msa0769960 AT1G67710.3 36.898 187 87 5 88 244 12 197 5.85e-21 93.2
Msa0769960 AT5G07210.1 26.523 279 140 7 14 230 16 291 2.12e-20 92.0
Msa0769960 AT1G49190.2 35.714 126 79 2 10 134 30 154 6.74e-18 84.7
Msa0769960 AT2G27070.1 25.753 299 158 9 1 242 3 294 1.69e-16 80.5
Msa0769960 AT5G59570.2 50.685 73 33 1 157 226 126 198 2.41e-16 78.6
Msa0769960 AT5G59570.1 50.685 73 33 1 157 226 126 198 2.41e-16 78.6
Msa0769960 AT2G40970.1 52.239 67 32 0 160 226 96 162 7.83e-16 76.3
Msa0769960 AT5G05090.1 52.857 70 32 1 158 226 69 138 1.09e-15 75.9
Msa0769960 AT3G46640.3 50.685 73 33 1 157 226 129 201 1.13e-15 76.6
Msa0769960 AT3G04030.1 57.812 64 26 1 159 221 35 98 1.43e-15 77.0
Msa0769960 AT3G46640.2 50.685 73 33 1 157 226 129 201 1.48e-15 76.3
Msa0769960 AT3G46640.1 50.685 73 33 1 157 226 129 201 1.48e-15 76.3
Msa0769960 AT3G04030.3 57.812 64 26 1 159 221 35 98 1.55e-15 77.0
Msa0769960 AT2G01060.1 52.941 85 36 3 158 238 4 88 1.59e-15 75.9
Msa0769960 AT1G68210.1 25.092 271 143 8 8 221 7 274 1.87e-15 77.4
Msa0769960 AT3G10760.1 59.322 59 24 0 168 226 104 162 2.26e-15 75.9
Msa0769960 AT5G18240.2 57.812 64 26 1 159 221 35 98 3.97e-15 75.9
Msa0769960 AT5G18240.3 57.812 64 26 1 159 221 35 98 3.97e-15 75.9
Msa0769960 AT5G18240.1 57.812 64 26 1 159 221 35 98 4.18e-15 75.9
Msa0769960 AT5G18240.4 57.812 64 26 1 159 221 35 98 4.18e-15 75.9
Msa0769960 AT5G18240.5 57.812 64 26 1 159 221 35 98 4.27e-15 75.9
Msa0769960 AT3G04030.2 53.968 63 29 0 159 221 35 97 1.59e-14 73.9
Msa0769960 AT4G04605.1 45.455 77 40 2 167 241 12 88 2.40e-14 71.2
Msa0769960 AT4G13640.1 41.346 104 51 3 169 268 37 134 3.56e-14 72.0
Msa0769960 AT4G13640.2 41.346 104 51 3 169 268 37 134 3.96e-14 72.0
Msa0769960 AT5G06800.1 40.323 124 62 5 169 284 192 311 5.81e-14 72.0
Msa0769960 AT5G44190.1 56.897 58 25 0 168 225 146 203 8.06e-14 72.0
Msa0769960 AT5G44190.2 56.897 58 25 0 168 225 146 203 9.68e-14 71.2
Msa0769960 AT3G24120.1 39.583 96 50 2 169 256 41 136 1.36e-13 70.5
Msa0769960 AT3G24120.2 39.583 96 50 2 169 256 41 136 1.40e-13 70.5
Msa0769960 AT5G06800.2 39.535 129 61 5 169 284 192 316 1.58e-13 70.9
Msa0769960 AT2G20570.1 47.500 80 36 1 152 225 130 209 2.96e-13 70.5
Msa0769960 AT2G40260.1 57.895 57 23 1 167 222 80 136 3.30e-13 70.1
Msa0769960 AT1G69580.1 37.302 126 68 5 165 284 30 150 4.03e-13 69.3
Msa0769960 AT1G14600.1 60.377 53 20 1 170 221 24 76 4.59e-13 68.6
Msa0769960 AT5G29000.4 37.615 109 60 2 129 236 198 299 6.69e-13 68.6
Msa0769960 AT2G20570.2 58.182 55 23 0 171 225 171 225 8.32e-13 68.9
Msa0769960 AT5G29000.1 36.607 112 63 2 129 239 155 259 1.20e-12 68.2
Msa0769960 AT5G29000.3 36.607 112 63 2 129 239 155 259 1.20e-12 68.2
Msa0769960 AT1G69580.2 39.252 107 58 4 165 269 30 131 1.21e-12 68.2
Msa0769960 AT5G29000.2 36.607 112 63 2 129 239 198 302 1.24e-12 68.6
Msa0769960 AT1G79430.2 43.299 97 51 3 157 252 22 115 1.30e-12 68.2
Msa0769960 AT4G28610.1 47.297 74 37 2 169 241 225 297 1.38e-12 68.2
Msa0769960 AT5G06800.3 53.968 63 27 2 161 221 183 245 1.92e-12 67.0
Msa0769960 AT3G13040.3 37.607 117 63 5 168 281 240 349 2.24e-12 67.8
Msa0769960 AT3G13040.1 37.607 117 63 5 168 281 240 349 2.24e-12 67.8
Msa0769960 AT3G13040.2 37.607 117 63 5 168 281 240 349 2.24e-12 67.8
Msa0769960 AT2G20400.1 41.304 92 51 2 169 259 231 320 2.65e-12 67.4
Msa0769960 AT2G02060.2 50.000 62 30 1 170 230 30 91 2.88e-12 66.2
Msa0769960 AT2G02060.1 50.000 62 30 1 170 230 30 91 2.88e-12 66.2
Msa0769960 AT2G42660.1 53.704 54 24 1 170 222 51 104 3.16e-12 65.9
Msa0769960 AT2G20400.2 59.259 54 21 1 169 221 231 284 3.19e-12 67.0
Msa0769960 AT2G38300.1 50.877 57 27 1 167 222 52 108 3.34e-12 66.6
Msa0769960 AT3G12730.1 55.556 54 23 1 169 221 23 76 3.87e-12 65.5
Msa0769960 AT5G45580.2 50.769 65 31 1 158 221 12 76 4.31e-12 65.9
Msa0769960 AT5G24470.1 29.134 127 85 3 13 135 49 174 4.93e-12 67.0
Msa0769960 AT3G04450.1 52.542 59 27 1 165 222 234 292 5.08e-12 66.6
Msa0769960 AT3G04450.4 52.542 59 27 1 165 222 194 252 5.56e-12 66.2
Msa0769960 AT3G04450.2 52.542 59 27 1 165 222 194 252 5.56e-12 66.2
Msa0769960 AT3G04450.3 52.542 59 27 1 165 222 234 292 5.59e-12 65.9
Msa0769960 AT5G45580.1 57.407 54 22 1 169 221 7 60 7.19e-12 65.1

Find 64 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAAAATTGGATGGAAAT+TGG 0.274934 5_2:+39885663 Msa0769960:CDS
CATATGGGGCTTGCGAATAT+TGG 0.282422 5_2:+39884941 Msa0769960:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 5_2:+39885346 Msa0769960:CDS
AATTCATCAGTTGATTATTT+CGG 0.292540 5_2:+39885753 Msa0769960:CDS
GCAATAACGTTGAGACCATT+AGG 0.294493 5_2:-39884451 None:intergenic
AAGATGATGCACAACAAAAT+TGG 0.295199 5_2:+39885652 Msa0769960:CDS
GAATTAATCACGACACAATC+TGG 0.322128 5_2:-39885735 None:intergenic
GAACGTCATCCTGTTGGTTC+CGG 0.354086 5_2:-39885587 None:intergenic
TTCTGATGCCTTGAACTTGT+TGG 0.365455 5_2:+39884659 Msa0769960:CDS
GTTACAAAGTCTATCACATA+TGG 0.379871 5_2:+39884925 Msa0769960:CDS
TCTTCAAAATATTGGAAGCT+TGG 0.394226 5_2:+39885041 Msa0769960:CDS
AATTCATAGGCATCCATGTT+TGG 0.395764 5_2:-39884726 None:intergenic
CTTGAACTTGTTGGAAAGAA+AGG 0.398808 5_2:+39884668 Msa0769960:CDS
ATCAGAAGCTGTATTGCATT+TGG 0.403201 5_2:-39884643 None:intergenic
CAAGTAACTAACCTTGATAA+TGG 0.405402 5_2:-39884528 None:intergenic
CTTTCTTTCCAACAAGTTCA+AGG 0.409488 5_2:-39884667 None:intergenic
CGGGAAAGAGAAGATGATAA+TGG 0.414808 5_2:+39885084 Msa0769960:CDS
CCCGCGGATAACTTTCATCT+TGG 0.418067 5_2:-39885626 None:intergenic
ACATCTCCTCAATGGTGTTA+AGG 0.419121 5_2:-39884495 None:intergenic
CAATAACGTTGAGACCATTA+GGG 0.421209 5_2:-39884450 None:intergenic
CTCAAGCTCAGCGGATTGAC+CGG 0.449486 5_2:+39885568 Msa0769960:CDS
GCGGATTGACCGGAACCAAC+AGG 0.466931 5_2:+39885578 Msa0769960:CDS
ATTGGAAGCTTGGATGTTCA+AGG 0.468841 5_2:+39885051 Msa0769960:CDS
ATGTTCAAGGTCATAGAAAA+CGG 0.468876 5_2:+39885064 Msa0769960:CDS
TTACAAAGTCTATCACATAT+GGG 0.475108 5_2:+39884926 Msa0769960:CDS
TGGCATCATGTTGTAAGAAA+AGG 0.476984 5_2:+39885000 Msa0769960:CDS
GACAACCCGTCATTCCCTAA+TGG 0.483413 5_2:+39884436 Msa0769960:CDS
TTGGACCAAGCCGTTGTATG+AGG 0.493917 5_2:+39884960 Msa0769960:CDS
CTCAATGGTGTTAAGGAAAG+TGG 0.494366 5_2:-39884488 None:intergenic
GCCTCGTTTGTCATGGTCAC+CGG 0.494740 5_2:+39885131 Msa0769960:CDS
AGGGAATGACGGGTTGTCAA+TGG 0.500611 5_2:-39884431 None:intergenic
TGTTCAAGGTCATAGAAAAC+GGG 0.501689 5_2:+39885065 Msa0769960:CDS
AGCATGTTGAACAAATTCAT+AGG 0.506691 5_2:-39884739 None:intergenic
ACACAATCTGGGAACAAGTT+GGG 0.508030 5_2:-39885723 None:intergenic
TTATGGGCTGTCAATCAACT+AGG 0.526348 5_2:+39885171 Msa0769960:CDS
TTGAATTGTTCCTCATACAA+CGG 0.530062 5_2:-39884970 None:intergenic
AGGAAAGAACCATACTATCA+AGG 0.531753 5_2:-39885194 None:intergenic
CACTTTCCTTAACACCATTG+AGG 0.536078 5_2:+39884489 Msa0769960:CDS
TGTAATCGATGCCATTATCA+AGG 0.541272 5_2:+39884517 Msa0769960:CDS
TGACGGGTTGTCAATGGAAA+GGG 0.552528 5_2:-39884425 None:intergenic
TGAGACCATTAGGGAATGAC+GGG 0.552778 5_2:-39884441 None:intergenic
GACACAATCTGGGAACAAGT+TGG 0.557153 5_2:-39885724 None:intergenic
TGATGCACAACAAAATTGGA+TGG 0.559172 5_2:+39885656 Msa0769960:CDS
TCGATTACACATCTCCTCAA+TGG 0.564409 5_2:-39884503 None:intergenic
AATTAATCACGACACAATCT+GGG 0.566059 5_2:-39885734 None:intergenic
TTTGTAACAGAACTTAATGT+GGG 0.567790 5_2:-39884910 None:intergenic
CATCTTGAACGTCATCCTGT+TGG 0.572615 5_2:-39885593 None:intergenic
GATGTTCATGGTTTGAGTGT+AGG 0.572619 5_2:+39885323 Msa0769960:CDS
TTGAGACCATTAGGGAATGA+CGG 0.573205 5_2:-39884442 None:intergenic
CGAAGAAGCCTCGTTTGTCA+TGG 0.575576 5_2:+39885124 Msa0769960:CDS
AACAGGAATATTGAGTTGTA+CGG 0.581006 5_2:-39884763 None:intergenic
CTTTGTAACAGAACTTAATG+TGG 0.582713 5_2:-39884911 None:intergenic
ATGACGGGTTGTCAATGGAA+AGG 0.593960 5_2:-39884426 None:intergenic
TTGTTCCTCATACAACGGCT+TGG 0.596667 5_2:-39884965 None:intergenic
AGGATGACGTTCAAGATGAG+TGG 0.604590 5_2:+39885598 Msa0769960:CDS
ATCAGTTGATTATTTCGGCT+AGG 0.608424 5_2:+39885758 Msa0769960:CDS
CCAAGATGAAAGTTATCCGC+GGG 0.608633 5_2:+39885626 Msa0769960:CDS
AGGAACAATTCAAGATGATG+TGG 0.609752 5_2:+39884980 Msa0769960:CDS
TGTGCATCATCTTGTACCCG+CGG 0.630231 5_2:-39885642 None:intergenic
TGATGTTCATATGCCAAACA+TGG 0.637365 5_2:+39884713 Msa0769960:CDS
TACAAAGTCTATCACATATG+GGG 0.656295 5_2:+39884927 Msa0769960:CDS
ACCAAGATGAAAGTTATCCG+CGG 0.659940 5_2:+39885625 Msa0769960:CDS
TCCGGTGACCATGACAAACG+AGG 0.694527 5_2:-39885132 None:intergenic
GTGATGACTCTCAAGCTCAG+CGG 0.726092 5_2:+39885559 Msa0769960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTTAAATTTAAATTTA+TGG + chr5_2:39885430-39885449 Msa0769960:intron 0.0%
!! TAAATTTAAATTTAAATTTA+TGG + chr5_2:39885430-39885449 Msa0769960:intron 0.0%
!! AATAAAAATCTTCAAAATAT+TGG + chr5_2:39885033-39885052 Msa0769960:CDS 10.0%
!! AATAAAAATCTTCAAAATAT+TGG + chr5_2:39885033-39885052 Msa0769960:CDS 10.0%
!!! TTAAAATTGTAATTTTCATG+TGG + chr5_2:39885459-39885478 Msa0769960:intron 15.0%
!!! TTAAAATTGTAATTTTCATG+TGG + chr5_2:39885459-39885478 Msa0769960:intron 15.0%
!! AAGAAAAACTTACTAATAAC+AGG - chr5_2:39884783-39884802 None:intergenic 20.0%
!! ACAAAGCAAAGAAAATAATT+AGG - chr5_2:39885217-39885236 None:intergenic 20.0%
!! TGTTTGTATTATTTAACAAC+TGG + chr5_2:39885234-39885253 Msa0769960:intron 20.0%
!!! ATCTTTTTGAGAATCATTTT+AGG - chr5_2:39885302-39885321 None:intergenic 20.0%
!! TAAAATGATTCTCAAAAAGA+TGG + chr5_2:39885301-39885320 Msa0769960:CDS 20.0%
!! AATTCATCAGTTGATTATTT+CGG + chr5_2:39885753-39885772 Msa0769960:CDS 20.0%
!! AAGAAAAACTTACTAATAAC+AGG - chr5_2:39884783-39884802 None:intergenic 20.0%
!! ACAAAGCAAAGAAAATAATT+AGG - chr5_2:39885217-39885236 None:intergenic 20.0%
!! TGTTTGTATTATTTAACAAC+TGG + chr5_2:39885234-39885253 Msa0769960:intron 20.0%
!!! ATCTTTTTGAGAATCATTTT+AGG - chr5_2:39885302-39885321 None:intergenic 20.0%
!! TAAAATGATTCTCAAAAAGA+TGG + chr5_2:39885301-39885320 Msa0769960:CDS 20.0%
!! AATTCATCAGTTGATTATTT+CGG + chr5_2:39885753-39885772 Msa0769960:CDS 20.0%
! AACACTAGAATTGATGAATA+AGG - chr5_2:39884564-39884583 None:intergenic 25.0%
! TTTGTAACAGAACTTAATGT+GGG - chr5_2:39884913-39884932 None:intergenic 25.0%
! TTACAAAGTCTATCACATAT+GGG + chr5_2:39884926-39884945 Msa0769960:CDS 25.0%
! TTAAAATCACACAAAACATG+TGG - chr5_2:39885413-39885432 None:intergenic 25.0%
! AAAAAAGTTGCGAATATTGT+CGG - chr5_2:39885694-39885713 None:intergenic 25.0%
! AACACTAGAATTGATGAATA+AGG - chr5_2:39884564-39884583 None:intergenic 25.0%
! TTTGTAACAGAACTTAATGT+GGG - chr5_2:39884913-39884932 None:intergenic 25.0%
! TTACAAAGTCTATCACATAT+GGG + chr5_2:39884926-39884945 Msa0769960:CDS 25.0%
! TTAAAATCACACAAAACATG+TGG - chr5_2:39885413-39885432 None:intergenic 25.0%
! AAAAAAGTTGCGAATATTGT+CGG - chr5_2:39885694-39885713 None:intergenic 25.0%
CAAGTAACTAACCTTGATAA+TGG - chr5_2:39884531-39884550 None:intergenic 30.0%
! CATTATCAAGGTTAGTTACT+TGG + chr5_2:39884529-39884548 Msa0769960:intron 30.0%
AGCATGTTGAACAAATTCAT+AGG - chr5_2:39884742-39884761 None:intergenic 30.0%
! AACAGGAATATTGAGTTGTA+CGG - chr5_2:39884766-39884785 None:intergenic 30.0%
CTTTGTAACAGAACTTAATG+TGG - chr5_2:39884914-39884933 None:intergenic 30.0%
GTTACAAAGTCTATCACATA+TGG + chr5_2:39884925-39884944 Msa0769960:CDS 30.0%
TACAAAGTCTATCACATATG+GGG + chr5_2:39884927-39884946 Msa0769960:CDS 30.0%
TTGAATTGTTCCTCATACAA+CGG - chr5_2:39884973-39884992 None:intergenic 30.0%
TCTTCAAAATATTGGAAGCT+TGG + chr5_2:39885041-39885060 Msa0769960:CDS 30.0%
ATGTTCAAGGTCATAGAAAA+CGG + chr5_2:39885064-39885083 Msa0769960:CDS 30.0%
! AACTGCATCAACGATTTTTA+TGG + chr5_2:39885154-39885173 Msa0769960:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG + chr5_2:39885155-39885174 Msa0769960:CDS 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG + chr5_2:39885271-39885290 Msa0769960:intron 30.0%
TCGATTGATGAGAAAAAATG+TGG + chr5_2:39885516-39885535 Msa0769960:CDS 30.0%
CGATTGATGAGAAAAAATGT+GGG + chr5_2:39885517-39885536 Msa0769960:CDS 30.0%
AAGATGATGCACAACAAAAT+TGG + chr5_2:39885652-39885671 Msa0769960:CDS 30.0%
CAACAAAATTGGATGGAAAT+TGG + chr5_2:39885663-39885682 Msa0769960:CDS 30.0%
AATTAATCACGACACAATCT+GGG - chr5_2:39885737-39885756 None:intergenic 30.0%
CAAGTAACTAACCTTGATAA+TGG - chr5_2:39884531-39884550 None:intergenic 30.0%
! CATTATCAAGGTTAGTTACT+TGG + chr5_2:39884529-39884548 Msa0769960:intron 30.0%
AGCATGTTGAACAAATTCAT+AGG - chr5_2:39884742-39884761 None:intergenic 30.0%
! AACAGGAATATTGAGTTGTA+CGG - chr5_2:39884766-39884785 None:intergenic 30.0%
CTTTGTAACAGAACTTAATG+TGG - chr5_2:39884914-39884933 None:intergenic 30.0%
GTTACAAAGTCTATCACATA+TGG + chr5_2:39884925-39884944 Msa0769960:CDS 30.0%
TACAAAGTCTATCACATATG+GGG + chr5_2:39884927-39884946 Msa0769960:CDS 30.0%
TTGAATTGTTCCTCATACAA+CGG - chr5_2:39884973-39884992 None:intergenic 30.0%
TCTTCAAAATATTGGAAGCT+TGG + chr5_2:39885041-39885060 Msa0769960:CDS 30.0%
ATGTTCAAGGTCATAGAAAA+CGG + chr5_2:39885064-39885083 Msa0769960:CDS 30.0%
! AACTGCATCAACGATTTTTA+TGG + chr5_2:39885154-39885173 Msa0769960:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG + chr5_2:39885155-39885174 Msa0769960:CDS 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG + chr5_2:39885271-39885290 Msa0769960:intron 30.0%
TCGATTGATGAGAAAAAATG+TGG + chr5_2:39885516-39885535 Msa0769960:CDS 30.0%
CGATTGATGAGAAAAAATGT+GGG + chr5_2:39885517-39885536 Msa0769960:CDS 30.0%
AAGATGATGCACAACAAAAT+TGG + chr5_2:39885652-39885671 Msa0769960:CDS 30.0%
CAACAAAATTGGATGGAAAT+TGG + chr5_2:39885663-39885682 Msa0769960:CDS 30.0%
AATTAATCACGACACAATCT+GGG - chr5_2:39885737-39885756 None:intergenic 30.0%
CAATAACGTTGAGACCATTA+GGG - chr5_2:39884453-39884472 None:intergenic 35.0%
TGTAATCGATGCCATTATCA+AGG + chr5_2:39884517-39884536 Msa0769960:CDS 35.0%
ATCAGAAGCTGTATTGCATT+TGG - chr5_2:39884646-39884665 None:intergenic 35.0%
CTTTCTTTCCAACAAGTTCA+AGG - chr5_2:39884670-39884689 None:intergenic 35.0%
!! CTTGAACTTGTTGGAAAGAA+AGG + chr5_2:39884668-39884687 Msa0769960:CDS 35.0%
TGATGTTCATATGCCAAACA+TGG + chr5_2:39884713-39884732 Msa0769960:CDS 35.0%
AATTCATAGGCATCCATGTT+TGG - chr5_2:39884729-39884748 None:intergenic 35.0%
ATTCAGTAGACATCACTACA+AGG - chr5_2:39884888-39884907 None:intergenic 35.0%
AGGAACAATTCAAGATGATG+TGG + chr5_2:39884980-39884999 Msa0769960:CDS 35.0%
TGGCATCATGTTGTAAGAAA+AGG + chr5_2:39885000-39885019 Msa0769960:CDS 35.0%
TGTTCAAGGTCATAGAAAAC+GGG + chr5_2:39885065-39885084 Msa0769960:CDS 35.0%
AAAAATCGTTGATGCAGTTC+CGG - chr5_2:39885153-39885172 None:intergenic 35.0%
AGGAAAGAACCATACTATCA+AGG - chr5_2:39885197-39885216 None:intergenic 35.0%
CTCAAAAAGATGGATGTTCA+TGG + chr5_2:39885311-39885330 Msa0769960:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr5_2:39885346-39885365 Msa0769960:CDS 35.0%
TGATGCACAACAAAATTGGA+TGG + chr5_2:39885656-39885675 Msa0769960:CDS 35.0%
GAATTAATCACGACACAATC+TGG - chr5_2:39885738-39885757 None:intergenic 35.0%
CAATAACGTTGAGACCATTA+GGG - chr5_2:39884453-39884472 None:intergenic 35.0%
TGTAATCGATGCCATTATCA+AGG + chr5_2:39884517-39884536 Msa0769960:CDS 35.0%
ATCAGAAGCTGTATTGCATT+TGG - chr5_2:39884646-39884665 None:intergenic 35.0%
CTTTCTTTCCAACAAGTTCA+AGG - chr5_2:39884670-39884689 None:intergenic 35.0%
!! CTTGAACTTGTTGGAAAGAA+AGG + chr5_2:39884668-39884687 Msa0769960:CDS 35.0%
TGATGTTCATATGCCAAACA+TGG + chr5_2:39884713-39884732 Msa0769960:CDS 35.0%
AATTCATAGGCATCCATGTT+TGG - chr5_2:39884729-39884748 None:intergenic 35.0%
ATTCAGTAGACATCACTACA+AGG - chr5_2:39884888-39884907 None:intergenic 35.0%
AGGAACAATTCAAGATGATG+TGG + chr5_2:39884980-39884999 Msa0769960:CDS 35.0%
TGGCATCATGTTGTAAGAAA+AGG + chr5_2:39885000-39885019 Msa0769960:CDS 35.0%
TGTTCAAGGTCATAGAAAAC+GGG + chr5_2:39885065-39885084 Msa0769960:CDS 35.0%
AAAAATCGTTGATGCAGTTC+CGG - chr5_2:39885153-39885172 None:intergenic 35.0%
AGGAAAGAACCATACTATCA+AGG - chr5_2:39885197-39885216 None:intergenic 35.0%
CTCAAAAAGATGGATGTTCA+TGG + chr5_2:39885311-39885330 Msa0769960:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr5_2:39885346-39885365 Msa0769960:CDS 35.0%
TGATGCACAACAAAATTGGA+TGG + chr5_2:39885656-39885675 Msa0769960:CDS 35.0%
GAATTAATCACGACACAATC+TGG - chr5_2:39885738-39885757 None:intergenic 35.0%
TTGAGACCATTAGGGAATGA+CGG - chr5_2:39884445-39884464 None:intergenic 40.0%
GCAATAACGTTGAGACCATT+AGG - chr5_2:39884454-39884473 None:intergenic 40.0%
! CTCAATGGTGTTAAGGAAAG+TGG - chr5_2:39884491-39884510 None:intergenic 40.0%
CACTTTCCTTAACACCATTG+AGG + chr5_2:39884489-39884508 Msa0769960:CDS 40.0%
! ACATCTCCTCAATGGTGTTA+AGG - chr5_2:39884498-39884517 None:intergenic 40.0%
TCGATTACACATCTCCTCAA+TGG - chr5_2:39884506-39884525 None:intergenic 40.0%
TTCTGATGCCTTGAACTTGT+TGG + chr5_2:39884659-39884678 Msa0769960:CDS 40.0%
ATTGGAAGCTTGGATGTTCA+AGG + chr5_2:39885051-39885070 Msa0769960:CDS 40.0%
CGGGAAAGAGAAGATGATAA+TGG + chr5_2:39885084-39885103 Msa0769960:CDS 40.0%
! TTATGGGCTGTCAATCAACT+AGG + chr5_2:39885171-39885190 Msa0769960:CDS 40.0%
! TCAACTAGGCCTTGATAGTA+TGG + chr5_2:39885185-39885204 Msa0769960:intron 40.0%
GATGTTCATGGTTTGAGTGT+AGG + chr5_2:39885323-39885342 Msa0769960:CDS 40.0%
ACCAAGATGAAAGTTATCCG+CGG + chr5_2:39885625-39885644 Msa0769960:CDS 40.0%
ACACAATCTGGGAACAAGTT+GGG - chr5_2:39885726-39885745 None:intergenic 40.0%
TTGAGACCATTAGGGAATGA+CGG - chr5_2:39884445-39884464 None:intergenic 40.0%
GCAATAACGTTGAGACCATT+AGG - chr5_2:39884454-39884473 None:intergenic 40.0%
! CTCAATGGTGTTAAGGAAAG+TGG - chr5_2:39884491-39884510 None:intergenic 40.0%
CACTTTCCTTAACACCATTG+AGG + chr5_2:39884489-39884508 Msa0769960:CDS 40.0%
! ACATCTCCTCAATGGTGTTA+AGG - chr5_2:39884498-39884517 None:intergenic 40.0%
TCGATTACACATCTCCTCAA+TGG - chr5_2:39884506-39884525 None:intergenic 40.0%
TTCTGATGCCTTGAACTTGT+TGG + chr5_2:39884659-39884678 Msa0769960:CDS 40.0%
ATTGGAAGCTTGGATGTTCA+AGG + chr5_2:39885051-39885070 Msa0769960:CDS 40.0%
CGGGAAAGAGAAGATGATAA+TGG + chr5_2:39885084-39885103 Msa0769960:CDS 40.0%
! TTATGGGCTGTCAATCAACT+AGG + chr5_2:39885171-39885190 Msa0769960:CDS 40.0%
! TCAACTAGGCCTTGATAGTA+TGG + chr5_2:39885185-39885204 Msa0769960:intron 40.0%
GATGTTCATGGTTTGAGTGT+AGG + chr5_2:39885323-39885342 Msa0769960:CDS 40.0%
ACCAAGATGAAAGTTATCCG+CGG + chr5_2:39885625-39885644 Msa0769960:CDS 40.0%
ACACAATCTGGGAACAAGTT+GGG - chr5_2:39885726-39885745 None:intergenic 40.0%
!! TGACGGGTTGTCAATGGAAA+GGG - chr5_2:39884428-39884447 None:intergenic 45.0%
!! ATGACGGGTTGTCAATGGAA+AGG - chr5_2:39884429-39884448 None:intergenic 45.0%
TGAGACCATTAGGGAATGAC+GGG - chr5_2:39884444-39884463 None:intergenic 45.0%
CATATGGGGCTTGCGAATAT+TGG + chr5_2:39884941-39884960 Msa0769960:CDS 45.0%
TTGTTCCTCATACAACGGCT+TGG - chr5_2:39884968-39884987 None:intergenic 45.0%
CATCTTGAACGTCATCCTGT+TGG - chr5_2:39885596-39885615 None:intergenic 45.0%
AGGATGACGTTCAAGATGAG+TGG + chr5_2:39885598-39885617 Msa0769960:CDS 45.0%
CCAAGATGAAAGTTATCCGC+GGG + chr5_2:39885626-39885645 Msa0769960:CDS 45.0%
GACACAATCTGGGAACAAGT+TGG - chr5_2:39885727-39885746 None:intergenic 45.0%
!! TGACGGGTTGTCAATGGAAA+GGG - chr5_2:39884428-39884447 None:intergenic 45.0%
!! ATGACGGGTTGTCAATGGAA+AGG - chr5_2:39884429-39884448 None:intergenic 45.0%
TGAGACCATTAGGGAATGAC+GGG - chr5_2:39884444-39884463 None:intergenic 45.0%
CATATGGGGCTTGCGAATAT+TGG + chr5_2:39884941-39884960 Msa0769960:CDS 45.0%
TTGTTCCTCATACAACGGCT+TGG - chr5_2:39884968-39884987 None:intergenic 45.0%
CATCTTGAACGTCATCCTGT+TGG - chr5_2:39885596-39885615 None:intergenic 45.0%
AGGATGACGTTCAAGATGAG+TGG + chr5_2:39885598-39885617 Msa0769960:CDS 45.0%
CCAAGATGAAAGTTATCCGC+GGG + chr5_2:39885626-39885645 Msa0769960:CDS 45.0%
GACACAATCTGGGAACAAGT+TGG - chr5_2:39885727-39885746 None:intergenic 45.0%
!! AGGGAATGACGGGTTGTCAA+TGG - chr5_2:39884434-39884453 None:intergenic 50.0%
GACAACCCGTCATTCCCTAA+TGG + chr5_2:39884436-39884455 Msa0769960:CDS 50.0%
TTGGACCAAGCCGTTGTATG+AGG + chr5_2:39884960-39884979 Msa0769960:CDS 50.0%
CGAAGAAGCCTCGTTTGTCA+TGG + chr5_2:39885124-39885143 Msa0769960:CDS 50.0%
GTGATGACTCTCAAGCTCAG+CGG + chr5_2:39885559-39885578 Msa0769960:CDS 50.0%
!! GAACGTCATCCTGTTGGTTC+CGG - chr5_2:39885590-39885609 None:intergenic 50.0%
CCCGCGGATAACTTTCATCT+TGG - chr5_2:39885629-39885648 None:intergenic 50.0%
TGTGCATCATCTTGTACCCG+CGG - chr5_2:39885645-39885664 None:intergenic 50.0%
!! AGGGAATGACGGGTTGTCAA+TGG - chr5_2:39884434-39884453 None:intergenic 50.0%
GACAACCCGTCATTCCCTAA+TGG + chr5_2:39884436-39884455 Msa0769960:CDS 50.0%
TTGGACCAAGCCGTTGTATG+AGG + chr5_2:39884960-39884979 Msa0769960:CDS 50.0%
CGAAGAAGCCTCGTTTGTCA+TGG + chr5_2:39885124-39885143 Msa0769960:CDS 50.0%
GTGATGACTCTCAAGCTCAG+CGG + chr5_2:39885559-39885578 Msa0769960:CDS 50.0%
!! GAACGTCATCCTGTTGGTTC+CGG - chr5_2:39885590-39885609 None:intergenic 50.0%
CCCGCGGATAACTTTCATCT+TGG - chr5_2:39885629-39885648 None:intergenic 50.0%
TGTGCATCATCTTGTACCCG+CGG - chr5_2:39885645-39885664 None:intergenic 50.0%
GCCTCGTTTGTCATGGTCAC+CGG + chr5_2:39885131-39885150 Msa0769960:CDS 55.0%
TCCGGTGACCATGACAAACG+AGG - chr5_2:39885135-39885154 None:intergenic 55.0%
!! CTCAAGCTCAGCGGATTGAC+CGG + chr5_2:39885568-39885587 Msa0769960:CDS 55.0%
GCCTCGTTTGTCATGGTCAC+CGG + chr5_2:39885131-39885150 Msa0769960:CDS 55.0%
TCCGGTGACCATGACAAACG+AGG - chr5_2:39885135-39885154 None:intergenic 55.0%
!! CTCAAGCTCAGCGGATTGAC+CGG + chr5_2:39885568-39885587 Msa0769960:CDS 55.0%
! GCGGATTGACCGGAACCAAC+AGG + chr5_2:39885578-39885597 Msa0769960:CDS 60.0%
! GCGGATTGACCGGAACCAAC+AGG + chr5_2:39885578-39885597 Msa0769960:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 39884421 39885779 39884421 ID=Msa0769960;Name=Msa0769960
chr5_2 mRNA 39884421 39885779 39884421 ID=Msa0769960-mRNA-1;Parent=Msa0769960;Name=Msa0769960-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|5|0|313
chr5_2 exon 39884421 39884538 39884421 ID=Msa0769960-mRNA-1:exon:12708;Parent=Msa0769960-mRNA-1
chr5_2 exon 39884640 39884789 39884640 ID=Msa0769960-mRNA-1:exon:12709;Parent=Msa0769960-mRNA-1
chr5_2 exon 39884893 39885201 39884893 ID=Msa0769960-mRNA-1:exon:12710;Parent=Msa0769960-mRNA-1
chr5_2 exon 39885291 39885367 39885291 ID=Msa0769960-mRNA-1:exon:12711;Parent=Msa0769960-mRNA-1
chr5_2 exon 39885492 39885779 39885492 ID=Msa0769960-mRNA-1:exon:12712;Parent=Msa0769960-mRNA-1
chr5_2 CDS 39884421 39884538 39884421 ID=Msa0769960-mRNA-1:cds;Parent=Msa0769960-mRNA-1
chr5_2 CDS 39884640 39884789 39884640 ID=Msa0769960-mRNA-1:cds;Parent=Msa0769960-mRNA-1
chr5_2 CDS 39884893 39885201 39884893 ID=Msa0769960-mRNA-1:cds;Parent=Msa0769960-mRNA-1
chr5_2 CDS 39885291 39885367 39885291 ID=Msa0769960-mRNA-1:cds;Parent=Msa0769960-mRNA-1
chr5_2 CDS 39885492 39885779 39885492 ID=Msa0769960-mRNA-1:cds;Parent=Msa0769960-mRNA-1
Gene Sequence

>Msa0769960

ATGGCCCTTTCCATTGACAACCCGTCATTCCCTAATGGTCTCAACGTTATTGCAATTGATCATGACACCACTTTCCTTAACACCATTGAGGAGATGTGTAATCGATGCCATTATCAAGTTACCAAATGCAATACAGCTTCTGATGCCTTGAACTTGTTGGAAAGAAAGGACTGTTTTGATGTAATGTTAATTGATGTTCATATGCCAAACATGGATGCCTATGAATTTGTTCAACATGCTACCGTACAACTCAATATTCCTGTTATTATGATGTCTACTGAATATCCCACATTAAGTTCTGTTACAAAGTCTATCACATATGGGGCTTGCGAATATTGGACCAAGCCGTTGTATGAGGAACAATTCAAGATGATGTGGCATCATGTTGTAAGAAAAGGCTTGAGCAAAAATAAAAATCTTCAAAATATTGGAAGCTTGGATGTTCAAGGTCATAGAAAACGGGAAAGAGAAGATGATAATGGATCAAAAGAATCAAATGCGAAGAAGCCTCGTTTGTCATGGTCACCGGAACTGCATCAACGATTTTTATGGGCTGTCAATCAACTAGGCCTTGATAAGGCAAAACCTAAAATGATTCTCAAAAAGATGGATGTTCATGGTTTGAGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGAAACTATTTGAAGAGATCGATTGATGAGAAAAAATGTGGGAAGAAAAAATCAAAGCTGAGTGATGACTCTCAAGCTCAGCGGATTGACCGGAACCAACAGGATGACGTTCAAGATGAGTGGATTGACCAAGATGAAAGTTATCCGCGGGTACAAGATGATGCACAACAAAATTGGATGGAAATTGGCAAATCCGACAATATTCGCAACTTTTTTTCTGACATACCCAACTTGTTCCCAGATTGTGTCGTGATTAATTCATCAGTTGATTATTTCGGCTAG
Protein sequence

>Msa0769960

MALSIDNPSFPNGLNVIAIDHDTTFLNTIEEMCNRCHYQVTKCNTASDALNLLERKDCFDVMLIDVHMPNMDAYEFVQHATVQLNIPVIMMSTEYPTLSSVTKSITYGACEYWTKPLYEEQFKMMWHHVVRKGLSKNKNLQNIGSLDVQGHRKREREDDNGSKESNAKKPRLSWSPELHQRFLWAVNQLGLDKAKPKMILKKMDVHGLSVGHVASHLQKYRNYLKRSIDEKKCGKKKSKLSDDSQAQRIDRNQQDDVQDEWIDQDESYPRVQDDAQQNWMEIGKSDNIRNFFSDIPNLFPDCVVINSSVDYFG*