AlfalfaGEDB | Msa0770140

M. sativa cultivar zhongmu-4 / Msa0770140

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0770140	sp\|Q9SZL8\|FRS5_ARATH	33.577	137	83	3	23	152	60	195	6.59e-13	71.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0770140	A0A2K3LRX3	64.626	147	48	1	1	143	1	147	5.05e-56	185

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TFs/TRs:

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Gene ID	Type	Classification
Msa0770140	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0770140	MtrunA17_Chr3g0082121	64.336	143	47	1	1	139	8	150	4.33e-53	182
Msa0770140	MtrunA17_Chr3g0082121	50.000	82	34	2	165	239	614	695	4.42e-13	68.2
Msa0770140	MtrunA17_Chr5g0401121	62.595	131	41	2	12	138	36	162	1.71e-44	147
Msa0770140	MtrunA17_Chr6g0458391	43.697	119	62	2	29	143	218	335	2.91e-21	92.4
Msa0770140	MtrunA17_Chr6g0458391	40.351	114	62	3	34	143	89	200	3.06e-15	74.7
Msa0770140	MtrunA17_Chr1g0203721	44.792	96	48	2	41	132	68	162	7.11e-18	82.4
Msa0770140	MtrunA17_Chr4g0033611	42.056	107	52	4	36	136	76	178	4.16e-16	75.5
Msa0770140	MtrunA17_Chr2g0324011	43.103	116	58	4	33	143	7	119	5.22e-16	77.0
Msa0770140	MtrunA17_Chr5g0448761	36.441	118	68	2	33	143	57	174	4.75e-15	74.3
Msa0770140	MtrunA17_Chr2g0329421	38.053	113	58	5	30	136	23	129	2.11e-14	69.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0770140	AT2G43280.1	34.591	159	89	6	34	179	20	176	1.57e-17	78.6
Msa0770140	AT3G59470.3	33.103	145	81	4	35	175	69	201	2.64e-15	73.6
Msa0770140	AT3G59470.1	33.103	145	81	4	35	175	69	201	2.64e-15	73.6
Msa0770140	AT3G59470.2	33.103	145	81	4	35	175	81	213	2.94e-15	73.6
Msa0770140	AT4G38180.1	33.577	137	83	3	23	152	60	195	6.70e-14	71.2
Msa0770140	AT3G07500.1	37.383	107	62	2	34	136	31	136	7.96e-12	63.2
Msa0770140	AT2G43280.2	31.690	142	82	6	51	179	2	141	9.08e-12	62.0
Msa0770140	AT4G12850.1	38.462	104	56	3	36	135	11	110	5.46e-11	60.5
Msa0770140	AT4G12850.4	38.462	104	56	3	36	135	11	110	6.58e-11	59.3
Msa0770140	AT4G12850.2	38.462	104	56	3	36	135	11	110	7.51e-11	58.9

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Find 48 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTCGTAAGCAGCTACCT+CGG	0.647055	5_2:-40248175	None:intergenic
AACGGGGTGTAATGCACGTA+TGG	0.472531	5_2:+40248365	Msa0770140:CDS
AATATTTGAGGAATTCCAAG+AGG	0.661269	5_2:+40248838	Msa0770140:CDS
AATGAGTATAGTAAAAGAAT+TGG	0.336174	5_2:+40248201	Msa0770140:CDS
ACAAAAGACATCAACCGACT+GGG	0.569421	5_2:+40248322	Msa0770140:CDS
ACAGAATTGTCTACCAAAAT+AGG	0.261577	5_2:-40248057	None:intergenic
ACGGGGTGTAATGCACGTAT+GGG	0.615409	5_2:+40248366	Msa0770140:CDS
ACTCTTGTGGCTGAAGAAGG+TGG	0.685524	5_2:-40248449	None:intergenic
AGAATCAAGTGGAATGGTTC+AGG	0.410213	5_2:+40249316	Msa0770140:CDS
AGACAATTCTGTTGATAAAT+TGG	0.199895	5_2:+40248068	Msa0770140:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAGAATTGTCTACCAAAAT+AGG	-	chr5_2:40248060-40248079	None:intergenic	30.0%
CACTATTCTTGAACAAAATG+AGG	+	chr5_2:40248095-40248114	Msa0770140:CDS	30.0%
TTTGGTATTCATCGAGAATA+TGG	+	chr5_2:40248225-40248244	Msa0770140:intron	30.0%
TTGGTATTCATCGAGAATAT+GGG	+	chr5_2:40248226-40248245	Msa0770140:intron	30.0%
GGGAATAAAAGCAAAAAAGA+CGG	+	chr5_2:40248246-40248265	Msa0770140:CDS	30.0%
GGAATAAAAGCAAAAAAGAC+GGG	+	chr5_2:40248247-40248266	Msa0770140:CDS	30.0%
GAATAAAAGCAAAAAAGACG+GGG	+	chr5_2:40248248-40248267	Msa0770140:CDS	30.0%
ATTTCACTTGATCGAAAGAT+AGG	+	chr5_2:40248390-40248409	Msa0770140:CDS	30.0%
TTTCACTTGATCGAAAGATA+GGG	+	chr5_2:40248391-40248410	Msa0770140:CDS	30.0%
ACCTAGATTGAAAATGAAGA+AGG	+	chr5_2:40248775-40248794	Msa0770140:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	40248045	40249424	40248045	ID=Msa0770140;Name=Msa0770140
chr5_2	mRNA	40248045	40249424	40248045	ID=Msa0770140-mRNA-1;Parent=Msa0770140;Name=Msa0770140-mRNA-1;_AED=0.23;_eAED=0.24;_QI=0\|0\|0\|1\|0\|0\|4\|0\|242
chr5_2	exon	40248045	40248228	40248045	ID=Msa0770140-mRNA-1:exon:12888;Parent=Msa0770140-mRNA-1
chr5_2	exon	40248241	40248473	40248241	ID=Msa0770140-mRNA-1:exon:12889;Parent=Msa0770140-mRNA-1
chr5_2	exon	40248797	40248905	40248797	ID=Msa0770140-mRNA-1:exon:12890;Parent=Msa0770140-mRNA-1
chr5_2	exon	40249225	40249424	40249225	ID=Msa0770140-mRNA-1:exon:12891;Parent=Msa0770140-mRNA-1
chr5_2	CDS	40248045	40248228	40248045	ID=Msa0770140-mRNA-1:cds;Parent=Msa0770140-mRNA-1
chr5_2	CDS	40248241	40248473	40248241	ID=Msa0770140-mRNA-1:cds;Parent=Msa0770140-mRNA-1
chr5_2	CDS	40248797	40248905	40248797	ID=Msa0770140-mRNA-1:cds;Parent=Msa0770140-mRNA-1
chr5_2	CDS	40249225	40249424	40249225	ID=Msa0770140-mRNA-1:cds;Parent=Msa0770140-mRNA-1

Gene Sequence

>Msa0770140

ATGAATCCGTCGCCTATTTTGGTAGACAATTCTGTTGATAAATTGGTAAACACTATTCTTGAACAAAATGAGGAACATGGACAAGAAAGTATTGACGACATCAAACCTCGCTTGGATATGATATTTGAATCCGAGGTAGCTGCTTACGAATTTTATAATGAGTATAGTAAAAGAATTGGATTTGAATATGGGAATAAAAGCAAAAAAGACGGGGTTTTAACTTCAAGAAGATTCACATGCTACAAAGAAGGTACACGAAGTGTTGACAAAAGACATCAACCGACTGGGGAGTCTACGGCAGAAACTAGAACGGGGTGTAATGCACGTATGGGTATTTCACTTGATCGAAAGATAGGGAAATACAAGGTTGTTGACTTTGTACTAGAACACAACCACCTTCTTCAGCCACAAGAGTATGCTCGAATGCTCGTCCAAGCCGGAAATGTTTACACCCCAAAAATATTTGAGGAATTCCAAGAGGAATACGAGGAATATCAAGACACTTGCATAAAGGTGTTGAAAGAAGAATCTCATGAAACTTACACTTTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAGTTGAAGATATGTTGGCCAAAAAATGTGTGGATGGAGAATCAAGTGGAATGGTTCAGGTAACCATATCACTTGGAAATGATAAAACTCATAACAGCGTGGAAACCGTTGTCAAGGCAAAAGGCATAAAGAAGCCAAATTGTTCA

Protein sequence

>Msa0770140

MNPSPILVDNSVDKLVNTILEQNEEHGQESIDDIKPRLDMIFESEVAAYEFYNEYSKRIGFEYGNKSKKDGVLTSRRFTCYKEGTRSVDKRHQPTGESTAETRTGCNARMGISLDRKIGKYKVVDFVLEHNHLLQPQEYARMLVQAGNVYTPKIFEEFQEEYEEYQDTCIKVLKEESHETYTFLSKVYEESSKIVEDMLAKKCVDGESSGMVQVTISLGNDKTHNSVETVVKAKGIKKPNCS