AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0771490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0771490 MtrunA17_Chr5g0424931 92.060 529 40 2 1 528 1 528 0.0 1001
Msa0771490 MtrunA17_Chr1g0178011 54.111 523 162 8 8 526 1 449 5.68e-176 503
Msa0771490 MtrunA17_Chr5g0417281 49.726 547 207 11 14 528 6 516 2.96e-158 461
Msa0771490 MtrunA17_Chr5g0436761 48.752 521 187 10 14 522 7 459 1.64e-152 444
Msa0771490 MtrunA17_Chr3g0115271 45.357 560 247 15 13 527 13 558 1.42e-143 426
Msa0771490 MtrunA17_Chr5g0424741 47.339 545 188 13 8 480 1 518 3.16e-142 420
Msa0771490 MtrunA17_Chr2g0277461 47.002 517 189 13 16 524 10 449 7.24e-133 394
Msa0771490 MtrunA17_Chr5g0424801 53.418 395 138 10 8 392 1 359 1.34e-129 382
Msa0771490 MtrunA17_Chr5g0425261 54.082 392 140 9 8 392 1 359 1.40e-129 382
Msa0771490 MtrunA17_Chr2g0289221 53.846 351 117 9 6 347 7 321 7.44e-117 349
Msa0771490 MtrunA17_Chr5g0417211 42.830 523 165 12 15 527 8 406 1.51e-115 347
Msa0771490 MtrunA17_Chr1g0181211 41.286 482 222 12 14 452 22 485 1.51e-101 320
Msa0771490 MtrunA17_Chr2g0326761 40.039 512 231 15 19 478 11 498 2.05e-99 315
Msa0771490 MtrunA17_Chr4g0028971 41.228 456 213 13 19 435 12 451 1.93e-95 305
Msa0771490 MtrunA17_Chr1g0199681 39.703 471 217 13 7 432 82 530 1.99e-95 304
Msa0771490 MtrunA17_Chr7g0264171 39.659 469 223 14 14 438 26 478 2.27e-95 302
Msa0771490 MtrunA17_Chr2g0277531 46.957 345 129 13 15 347 16 318 1.83e-86 270
Msa0771490 MtrunA17_Chr5g0425661 61.111 198 69 3 16 208 34 228 4.51e-79 248
Msa0771490 MtrunA17_Chr7g0264161 40.483 331 154 9 29 347 2 301 9.20e-70 226
Msa0771490 MtrunA17_Chr2g0282961 34.037 379 194 9 10 367 44 387 1.59e-64 221
Msa0771490 MtrunA17_Chr2g0276781 35.356 379 207 7 19 386 23 374 2.77e-61 213
Msa0771490 MtrunA17_Chr8g0385791 34.715 386 201 10 19 386 59 411 1.64e-60 213
Msa0771490 MtrunA17_Chr2g0285461 35.910 401 206 12 6 385 6 376 5.14e-60 213
Msa0771490 MtrunA17_Chr4g0046371 34.409 372 188 9 19 367 38 376 1.38e-59 209
Msa0771490 MtrunA17_Chr4g0070181 35.389 373 191 8 14 369 18 357 2.06e-59 213
Msa0771490 MtrunA17_Chr4g0009751 34.817 382 206 10 19 386 19 371 4.11e-59 206
Msa0771490 MtrunA17_Chr2g0301251 34.595 370 198 9 14 369 18 357 9.98e-59 210
Msa0771490 MtrunA17_Chr5g0433301 34.872 390 201 11 16 385 21 377 5.01e-58 207
Msa0771490 MtrunA17_Chr3g0109391 34.359 390 203 10 16 385 23 379 8.47e-58 206
Msa0771490 MtrunA17_Chr1g0156321 35.405 370 192 9 14 367 38 376 1.73e-57 205
Msa0771490 MtrunA17_Chr8g0376771 34.750 400 211 11 6 385 6 375 2.13e-57 206
Msa0771490 MtrunA17_Chr7g0239061 32.225 391 223 10 19 396 14 375 5.51e-56 199
Msa0771490 MtrunA17_Chr8g0389081 34.146 369 201 9 16 370 29 369 1.61e-55 201
Msa0771490 MtrunA17_Chr8g0346711 32.333 433 224 14 19 439 29 404 1.96e-54 194
Msa0771490 MtrunA17_Chr2g0326281 32.840 405 206 13 19 389 56 428 5.20e-54 195
Msa0771490 MtrunA17_Chr4g0029671 35.204 392 201 11 19 386 54 416 6.52e-53 192
Msa0771490 MtrunA17_Chr5g0425651 74.107 112 25 1 264 371 1 112 5.13e-51 170
Msa0771490 MtrunA17_Chr5g0424791 47.020 151 64 5 68 213 12 151 3.59e-31 118
Msa0771490 MtrunA17_Chr8g0356211 27.160 243 153 5 125 367 2 220 2.94e-22 97.4
Msa0771490 MtrunA17_Chr5g0419861 39.130 115 57 4 57 163 12 121 7.95e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0771490 AT1G77850.1 39.092 573 287 12 1 527 1 557 9.05e-123 372
Msa0771490 AT1G77850.2 39.092 573 287 12 1 527 1 557 1.76e-122 372
Msa0771490 AT4G30080.1 39.241 553 276 18 19 527 19 555 6.90e-97 308
Msa0771490 AT2G28350.2 39.440 464 208 13 19 432 10 450 4.85e-89 287
Msa0771490 AT2G28350.1 39.785 465 205 14 19 432 10 450 8.19e-89 287
Msa0771490 AT2G46530.1 36.842 361 193 8 18 370 20 353 3.65e-65 223
Msa0771490 AT2G46530.3 36.872 358 191 8 18 367 41 371 6.72e-65 222
Msa0771490 AT2G33860.1 34.865 370 185 7 19 367 54 388 1.97e-62 215
Msa0771490 AT3G61830.1 34.840 376 200 9 15 382 21 359 5.58e-62 214
Msa0771490 AT1G30330.3 35.733 389 203 10 14 385 20 378 5.33e-60 213
Msa0771490 AT1G30330.2 35.733 389 203 10 14 385 20 378 5.33e-60 213
Msa0771490 AT1G30330.1 35.052 388 207 9 14 385 18 376 5.47e-60 213
Msa0771490 AT5G37020.2 33.911 404 215 11 16 400 19 389 1.69e-59 210
Msa0771490 AT5G37020.1 33.911 404 215 11 16 400 19 389 3.80e-59 210
Msa0771490 AT4G23980.2 30.801 487 279 13 19 484 12 461 2.07e-58 205
Msa0771490 AT5G62000.4 33.083 399 222 9 3 386 45 413 3.56e-58 207
Msa0771490 AT5G62000.2 33.083 399 222 9 3 386 45 413 4.22e-58 207
Msa0771490 AT5G62000.5 33.083 399 222 9 3 386 45 413 4.22e-58 207
Msa0771490 AT5G62000.3 33.083 399 222 9 3 386 45 413 4.22e-58 207
Msa0771490 AT5G62000.1 33.083 399 222 9 3 386 45 413 4.22e-58 207
Msa0771490 AT5G60450.2 35.115 393 198 14 13 383 35 392 4.80e-58 206
Msa0771490 AT5G60450.1 35.128 390 196 14 16 383 62 416 1.25e-57 205
Msa0771490 AT1G59750.4 33.791 364 204 6 19 372 22 358 3.61e-57 202
Msa0771490 AT1G59750.1 33.791 364 204 6 19 372 22 358 3.70e-57 202
Msa0771490 AT1G59750.3 33.791 364 204 6 19 372 22 358 3.70e-57 202
Msa0771490 AT1G59750.2 33.791 364 204 6 19 372 22 358 3.88e-57 202
Msa0771490 AT4G23980.1 33.679 386 211 9 19 390 12 366 4.49e-57 201
Msa0771490 AT5G20730.2 34.667 375 191 9 14 369 19 358 7.92e-57 205
Msa0771490 AT1G19220.1 34.794 388 204 11 16 386 20 375 1.05e-56 205
Msa0771490 AT5G20730.3 34.574 376 191 10 14 369 19 359 2.92e-55 201
Msa0771490 AT5G20730.1 34.574 376 191 10 14 369 19 359 3.23e-55 201
Msa0771490 AT1G19850.2 35.054 368 193 11 16 367 40 377 8.19e-55 198
Msa0771490 AT1G19850.1 35.054 368 193 11 16 367 51 388 1.05e-54 198
Msa0771490 AT1G34310.1 32.902 386 215 9 16 390 24 376 4.17e-54 192
Msa0771490 AT1G34390.1 32.216 388 215 10 19 398 27 374 4.52e-54 192
Msa0771490 AT1G34410.1 31.486 397 229 10 19 406 27 389 2.24e-53 191
Msa0771490 AT1G35520.1 32.898 383 213 10 19 390 27 376 5.11e-53 189
Msa0771490 AT1G35540.1 32.283 381 218 9 19 390 27 376 3.55e-52 187
Msa0771490 AT1G35240.1 31.593 383 218 10 19 390 27 376 6.88e-50 181
Msa0771490 AT3G61830.2 34.234 333 178 8 54 382 13 308 7.71e-48 174
Msa0771490 AT1G34170.1 27.833 503 269 16 19 487 25 467 2.03e-46 169
Msa0771490 AT2G46530.2 36.822 258 137 4 114 370 34 266 6.22e-45 166
Msa0771490 AT2G33860.2 34.231 260 132 4 117 367 2 231 3.49e-44 162
Msa0771490 AT1G34170.3 26.186 527 266 17 19 508 25 465 5.48e-41 155
Msa0771490 AT1G34170.2 26.640 503 249 16 19 487 25 441 5.50e-40 151
Msa0771490 AT5G37020.3 33.788 293 156 8 117 400 31 294 8.02e-40 154
Msa0771490 AT1G43950.1 38.049 205 111 6 19 216 27 222 1.32e-36 135
Msa0771490 ATMG00940.1 35.821 134 73 5 204 328 1 130 1.33e-15 75.1

Find 146 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTTCAATTAAGTTATT+TGG 0.154755 5_2:+44213245 Msa0771490:CDS
GGCACATGTCTGCCAGATTT+TGG 0.193688 5_2:-44210128 None:intergenic
TTCAAACTACTAATGTTATT+TGG 0.205378 5_2:-44213167 None:intergenic
ACAGTGATTGTTTGAGGGTT+AGG 0.269069 5_2:-44210232 None:intergenic
CCCTAATTGGCGCATGCTTC+AGG 0.273598 5_2:+44211092 Msa0771490:CDS
CATGATCATTTATCTCTATC+AGG 0.276134 5_2:+44213059 Msa0771490:CDS
TATATTACCCTAGGGCTAAT+TGG 0.305719 5_2:+44210911 Msa0771490:CDS
AATCAAAGGCAGTGAATAAT+AGG 0.317388 5_2:+44210749 Msa0771490:CDS
AACCGCAATTCCACAATGTT+TGG 0.327821 5_2:-44213007 None:intergenic
CAGTGATTGTTTGAGGGTTA+GGG 0.331173 5_2:-44210231 None:intergenic
GTCAATGCATCAAATTTCAT+TGG 0.331745 5_2:-44213339 None:intergenic
CTGATGTTCATGACGGAGTT+TGG 0.334825 5_2:+44210554 Msa0771490:CDS
TACCGGACTGAGTAGCAATT+TGG 0.335469 5_2:-44210338 None:intergenic
ATTAGTAGTTTGAACACCTT+TGG 0.337278 5_2:+44213176 Msa0771490:CDS
CAAACAATCACTGTTATTGA+TGG 0.342498 5_2:+44210241 Msa0771490:CDS
ATGAAGATTGGTTGGGCATC+TGG 0.343084 5_2:+44210973 Msa0771490:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.343626 5_2:-44211093 None:intergenic
TGCGTCAATAACCGCCTTCT+CGG 0.347408 5_2:-44210851 None:intergenic
AGTTCTTGTCCTTCAGTTAC+CGG 0.352672 5_2:-44210355 None:intergenic
AATTTCATTGGATCCCTTAG+AGG 0.360856 5_2:-44213327 None:intergenic
CTGGCTCCCTGCATGCCTCC+TGG 0.375810 5_2:-44213035 None:intergenic
GGAGAATTTAAGTCAAGCTT+GGG 0.377595 5_2:-44210505 None:intergenic
CCTGAAGCATGCGCCAATTA+GGG 0.379672 5_2:-44211092 None:intergenic
TAAACTTTAGAACGTACCTT+TGG 0.384455 5_2:-44210169 None:intergenic
GTTTGGTTCAAAGATCTCAT+AGG 0.385258 5_2:-44212990 None:intergenic
GAGTCCAACCAGTAGTAAAA+AGG 0.388502 5_2:-44210615 None:intergenic
TCTTTGAATGATAATAGTTA+TGG 0.390167 5_2:+44213092 Msa0771490:CDS
GGAGTTGGCGGAAAAGAATA+TGG 0.402969 5_2:+44210876 Msa0771490:CDS
GGGACAGACATGGTGGCCGG+TGG 0.403787 5_2:-44210085 None:intergenic
CATCACGGCCGTTGATCTCC+TGG 0.419001 5_2:+44210288 Msa0771490:CDS
ATGTGTCCAGTGTCCCTAAT+TGG 0.419261 5_2:+44211079 Msa0771490:CDS
GAAAGTGATGTCCATCATTC+TGG 0.421797 5_2:+44213287 Msa0771490:CDS
CAGGAGGCATGCAGGGAGCC+AGG 0.425900 5_2:+44213036 Msa0771490:CDS
ACTATTATCATTCAAAGAGT+TGG 0.428670 5_2:-44213087 None:intergenic
GCCGCGTTAAATCCAAAATC+TGG 0.431371 5_2:+44210116 Msa0771490:CDS
ATCAAAACCGCGTCAATCCT+TGG 0.437926 5_2:+44212835 Msa0771490:CDS
CAAGGGAGAAGATTCCTCTA+AGG 0.441291 5_2:+44213313 Msa0771490:CDS
TCAATAACAGTGATTGTTTG+AGG 0.447219 5_2:-44210238 None:intergenic
TTGAAGTTATATATTACCCT+AGG 0.447607 5_2:+44210902 Msa0771490:CDS
AGGAGAATTTAAGTCAAGCT+TGG 0.457295 5_2:-44210506 None:intergenic
CGCCAAATTGCTACTCAGTC+CGG 0.457882 5_2:+44210336 Msa0771490:CDS
TTAGTAGTTTGAACACCTTT+GGG 0.458729 5_2:+44213177 Msa0771490:CDS
ACAAAATCTGATTCTAATAA+CGG 0.462788 5_2:+44210439 Msa0771490:CDS
GAACTCTATCATAGGAATGA+AGG 0.465037 5_2:-44212967 None:intergenic
AACACAAAAGAAGTTGGTTC+TGG 0.471305 5_2:+44213227 Msa0771490:CDS
ATTGTTTGAGGGTTAGGGGA+AGG 0.473070 5_2:-44210226 None:intergenic
TTGTTTGAGGGTTAGGGGAA+GGG 0.475352 5_2:-44210225 None:intergenic
CTCATAGGGAACTCTATCAT+AGG 0.480052 5_2:-44212975 None:intergenic
CAATAACAGTGATTGTTTGA+GGG 0.482943 5_2:-44210237 None:intergenic
GTGGATGATGCTATGAAGAT+TGG 0.483610 5_2:+44210961 Msa0771490:CDS
TCTTCTTTATCAACAACCTC+AGG 0.486494 5_2:-44210379 None:intergenic
TTACTTCCCACAAGGTCACT+TGG 0.494429 5_2:+44210192 Msa0771490:CDS
TGGAACGTTCACCGATGGAC+CGG 0.495600 5_2:-44210149 None:intergenic
TTGGATTTAACGCGGCGTGC+CGG 0.498738 5_2:-44210109 None:intergenic
GGCCACCATGTCTGTCCCTC+CGG 0.501925 5_2:+44210090 Msa0771490:CDS
GGTTATTGACGCAGTGGAGT+TGG 0.506246 5_2:+44210861 Msa0771490:CDS
TTGAGGGTTAGGGGAAGGGC+AGG 0.507377 5_2:-44210221 None:intergenic
TAAAGAAGAAGACGGTGATA+AGG 0.507483 5_2:+44210393 Msa0771490:CDS
GGGGATGAGGGTAAAGAGAA+TGG 0.508401 5_2:+44210775 Msa0771490:CDS
GATTTAACGCGGCGTGCCGG+AGG 0.509373 5_2:-44210106 None:intergenic
ACCTTTGGAACGTTCACCGA+TGG 0.510835 5_2:-44210154 None:intergenic
TTTGGTTCAAAGATCTCATA+GGG 0.511861 5_2:-44212989 None:intergenic
TTCCGCCATGTCTTCCCCAC+CGG 0.514610 5_2:+44210069 None:intergenic
AAAATCTGGCAGACATGTGC+CGG 0.515291 5_2:+44210130 Msa0771490:CDS
TGAGGGTAAAGAGAATGGTT+TGG 0.516087 5_2:+44210780 Msa0771490:CDS
GGACAGACATGGTGGCCGGT+GGG 0.516458 5_2:-44210084 None:intergenic
AGTGATTGTTTGAGGGTTAG+GGG 0.517041 5_2:-44210230 None:intergenic
GCAGACATGTGCCGGTCCAT+CGG 0.518088 5_2:+44210138 Msa0771490:CDS
AACTCTATCATAGGAATGAA+GGG 0.519149 5_2:-44212966 None:intergenic
GGTGGCCGGTGGGGAAGACA+TGG 0.519543 5_2:-44210074 None:intergenic
CTCCCTTGCTACCAGAATGA+TGG 0.521361 5_2:-44213298 None:intergenic
ACTGCCGGAGATATAGTTGT+TGG 0.521728 5_2:+44210694 Msa0771490:CDS
TCTGATTCTAATAACGGCGG+TGG 0.523119 5_2:+44210445 Msa0771490:CDS
GAAGATTCATCAATCTTCAA+AGG 0.524456 5_2:-44211012 None:intergenic
GACAGACATGGTGGCCGGTG+GGG 0.526151 5_2:-44210083 None:intergenic
GACCTCATCGGTGTGAGGAT+CGG 0.526510 5_2:-44210311 None:intergenic
GTAGAATCCTGAACTAGTCT+AGG 0.529298 5_2:-44212918 None:intergenic
AACAGCAACACAAAAGAAGT+TGG 0.534960 5_2:+44213221 Msa0771490:CDS
GGAGGGACAGACATGGTGGC+CGG 0.536529 5_2:-44210088 None:intergenic
GGTTTGGAGGTCAAAGGAGA+GGG 0.536796 5_2:+44210796 Msa0771490:CDS
GAGAGTTCCTGAGATGGAAA+AGG 0.537940 5_2:-44210526 None:intergenic
AGAAATGGTAGATGCACTGC+AGG 0.541883 5_2:-44212882 None:intergenic
GATTCCAACAACTATATCTC+CGG 0.544415 5_2:-44210698 None:intergenic
CATGTTCCAAGTGACCTTGT+GGG 0.545385 5_2:-44210198 None:intergenic
AAGGGAGAAGATTCCTCTAA+GGG 0.546926 5_2:+44213314 Msa0771490:CDS
TATTGACGCAGTGGAGTTGG+CGG 0.549772 5_2:+44210864 Msa0771490:CDS
CATCGGTGTGAGGATCGGCC+AGG 0.550560 5_2:-44210306 None:intergenic
TGGTTTGGAGGTCAAAGGAG+AGG 0.550776 5_2:+44210795 Msa0771490:CDS
GAAATAAGTTCAACTTGCCA+AGG 0.551283 5_2:-44212852 None:intergenic
TAGAGATAAATGATCATGCC+TGG 0.553837 5_2:-44213054 None:intergenic
GTCCATCATTCTGGTAGCAA+GGG 0.557565 5_2:+44213296 Msa0771490:CDS
TCAACGGCCGTGATGATACA+AGG 0.557986 5_2:-44210280 None:intergenic
AACCAAACATTGTGGAATTG+CGG 0.559556 5_2:+44213005 Msa0771490:CDS
ATGATGCTATGAAGATTGGT+TGG 0.562627 5_2:+44210965 Msa0771490:CDS
TAGATGCACTGCAGGATTGT+GGG 0.562741 5_2:-44212874 None:intergenic
GAGAATGGTTTGGAGGTCAA+AGG 0.563275 5_2:+44210790 Msa0771490:CDS
TGAAGTTATATATTACCCTA+GGG 0.565837 5_2:+44210903 Msa0771490:CDS
GTTTACTGCAGCTGAATCAA+AGG 0.566092 5_2:+44210735 Msa0771490:CDS
TGGCGACAAGTCGTTAGATG+TGG 0.568778 5_2:-44213147 None:intergenic
GCATGTTCCAAGTGACCTTG+TGG 0.569473 5_2:-44210199 None:intergenic
GGGTAAAGAGAATGGTTTGG+AGG 0.569581 5_2:+44210783 Msa0771490:CDS
AAATTGCACCAATTAGCCCT+AGG 0.571867 5_2:-44210919 None:intergenic
CAGTAAACTTCATGTTTCTG+CGG 0.572135 5_2:-44210720 None:intergenic
GTTGTTGATAAAGAAGAAGA+CGG 0.573045 5_2:+44210385 Msa0771490:CDS
TGTAGTAGATGCAAATGTTG+TGG 0.577875 5_2:+44210942 Msa0771490:CDS
CTACTCAGTCCGGTAACTGA+AGG 0.578098 5_2:+44210346 Msa0771490:CDS
TGATGCTATGAAGATTGGTT+GGG 0.580467 5_2:+44210966 Msa0771490:CDS
AAAGGCAGTGAATAATAGGA+AGG 0.580552 5_2:+44210753 Msa0771490:CDS
TGTCCATCATTCTGGTAGCA+AGG 0.582395 5_2:+44213295 Msa0771490:CDS
GTGATGATACAAGGAAAAGA+AGG 0.595656 5_2:-44210271 None:intergenic
ATTTAACGCGGCGTGCCGGA+GGG 0.596031 5_2:-44210105 None:intergenic
GGCGACAAGTCGTTAGATGT+GGG 0.596585 5_2:-44213146 None:intergenic
CATGCGCCAATTAGGGACAC+TGG 0.600814 5_2:-44211085 None:intergenic
GATCTTTGAACCAAACATTG+TGG 0.601993 5_2:+44212997 Msa0771490:CDS
AGGCAGTGAATAATAGGAAG+GGG 0.602083 5_2:+44210755 Msa0771490:CDS
TCCATCGGTGAACGTTCCAA+AGG 0.605082 5_2:+44210153 Msa0771490:CDS
CTCAACAAAATCTATCCCAA+AGG 0.608187 5_2:-44213192 None:intergenic
AAGTTTGCTCATGTCTATCG+AGG 0.613801 5_2:+44210577 Msa0771490:CDS
GTAGATGCACTGCAGGATTG+TGG 0.617995 5_2:-44212875 None:intergenic
AAGGCAGTGAATAATAGGAA+GGG 0.619996 5_2:+44210754 Msa0771490:CDS
AGGATCGGCCAGGAGATCAA+CGG 0.621476 5_2:-44210296 None:intergenic
GGCCGATCCTCACACCGATG+AGG 0.626120 5_2:+44210309 Msa0771490:CDS
CAACTTGCCAAGGATTGACG+CGG 0.626664 5_2:-44212842 None:intergenic
GTAACGGAGAGTTCCTGAGA+TGG 0.631803 5_2:-44210532 None:intergenic
CAATTTGGCGAAGACCTCAT+CGG 0.632488 5_2:-44210323 None:intergenic
AAGGGGAATACTAACCGAGA+AGG 0.636629 5_2:+44210837 Msa0771490:CDS
AATTGCACCAATTAGCCCTA+GGG 0.639117 5_2:-44210918 None:intergenic
GCGTGCCGGAGGGACAGACA+TGG 0.644657 5_2:-44210095 None:intergenic
ATCAAGTCAGCACAAAGGCG+AGG 0.645484 5_2:-44210478 None:intergenic
TCCGTCATGAACATCAGTAA+CGG 0.649838 5_2:-44210548 None:intergenic
GCGAAGACCTCATCGGTGTG+AGG 0.652471 5_2:-44210316 None:intergenic
AGTTTGCTCATGTCTATCGA+GGG 0.654001 5_2:+44210578 Msa0771490:CDS
TCCGTTACTGATGTTCATGA+CGG 0.654864 5_2:+44210547 Msa0771490:CDS
AAAGTTTATTACTTCCCACA+AGG 0.663461 5_2:+44210184 Msa0771490:CDS
GGGAATACTAACCGAGAAGG+CGG 0.664523 5_2:+44210840 Msa0771490:CDS
ACATTAGAGATTGTTCCTTG+AGG 0.666803 5_2:-44211060 None:intergenic
GAAGGCGGTTATTGACGCAG+TGG 0.667200 5_2:+44210855 Msa0771490:CDS
GGCAGTGAATAATAGGAAGG+GGG 0.673747 5_2:+44210756 Msa0771490:CDS
ACTCTATCATAGGAATGAAG+GGG 0.674109 5_2:-44212965 None:intergenic
TGATACAAGGAAAAGAAGGA+CGG 0.677808 5_2:-44210267 None:intergenic
TGCCGGAGGGACAGACATGG+TGG 0.679386 5_2:-44210092 None:intergenic
ACTCTTGTTATCACTAAACA+AGG 0.684372 5_2:-44212940 None:intergenic
TGAAGGACAAGAACTTCCTG+AGG 0.686819 5_2:+44210363 Msa0771490:CDS
GAGAATTTAAGTCAAGCTTG+GGG 0.690174 5_2:-44210504 None:intergenic
AAATCTGATTCTAATAACGG+CGG 0.706742 5_2:+44210442 Msa0771490:CDS
GGAAGATCAAGTCAGCACAA+AGG 0.715164 5_2:-44210483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTATTTTTTGAATCA+AGG + chr5_2:44211144-44211163 Msa0771490:intron 10.0%
!!! TATTTTATTTTTTGAATCAA+GGG + chr5_2:44211145-44211164 Msa0771490:intron 10.0%
!! CAATTTAGTATATAAATATT+AGG + chr5_2:44212136-44212155 Msa0771490:intron 10.0%
!!! TGCATTTTATTATAAAAATA+TGG - chr5_2:44212635-44212654 None:intergenic 10.0%
!!! TCAATATAATCCTTTTAATT+AGG - chr5_2:44211354-44211373 None:intergenic 15.0%
!!! AATTACTTGTATTAGAATTT+TGG - chr5_2:44211771-44211790 None:intergenic 15.0%
!!! TAATTGTTGAATAATGTTTT+CGG - chr5_2:44211856-44211875 None:intergenic 15.0%
!! TTATATACTAAATTGATCAA+AGG - chr5_2:44212131-44212150 None:intergenic 15.0%
!!! ATATTTTGAATGACTAATAT+TGG + chr5_2:44212750-44212769 Msa0771490:intron 15.0%
!! TATGATAAAAGAGATAAAAA+TGG - chr5_2:44212783-44212802 None:intergenic 15.0%
!!! ATTTTTATCTCTTTTATCAT+AGG + chr5_2:44212782-44212801 Msa0771490:intron 15.0%
!! ACAAAATCTGATTCTAATAA+CGG + chr5_2:44210439-44210458 Msa0771490:CDS 20.0%
!! ATTGTTACTACCTAATTAAA+AGG + chr5_2:44211341-44211360 Msa0771490:intron 20.0%
!!! AAGTGTAAAAAAAAGGTTTT+CGG - chr5_2:44211404-44211423 None:intergenic 20.0%
!! GAAAAGTAAGTGTAAAAAAA+AGG - chr5_2:44211411-44211430 None:intergenic 20.0%
!! AAAACATGAATCTTTAAACT+TGG - chr5_2:44212106-44212125 None:intergenic 20.0%
!! TATTTATATCTTATCCCATA+AGG - chr5_2:44212208-44212227 None:intergenic 20.0%
!!! GTCGTTAATTTACTATTTTT+TGG + chr5_2:44212259-44212278 Msa0771490:intron 20.0%
!!! TCTTTTATCATAGGTTAATT+GGG + chr5_2:44212791-44212810 Msa0771490:intron 20.0%
!! TCTTTGAATGATAATAGTTA+TGG + chr5_2:44213092-44213111 Msa0771490:CDS 20.0%
!! TTCAAACTACTAATGTTATT+TGG - chr5_2:44213170-44213189 None:intergenic 20.0%
! TTGAAGTTATATATTACCCT+AGG + chr5_2:44210902-44210921 Msa0771490:CDS 25.0%
! TGAAGTTATATATTACCCTA+GGG + chr5_2:44210903-44210922 Msa0771490:CDS 25.0%
! AATTATTGTCGAAGAAGATT+AGG + chr5_2:44211196-44211215 Msa0771490:intron 25.0%
!! AGTGTAAAAAAAAGGTTTTC+GGG - chr5_2:44211403-44211422 None:intergenic 25.0%
! TTACCTTCAACACTAATAAA+AGG + chr5_2:44212294-44212313 Msa0771490:intron 25.0%
! TACCTTCAACACTAATAAAA+GGG + chr5_2:44212295-44212314 Msa0771490:intron 25.0%
!!! CCATGATTATGGATTAATTT+TGG - chr5_2:44212416-44212435 None:intergenic 25.0%
! CCAAAATTAATCCATAATCA+TGG + chr5_2:44212413-44212432 Msa0771490:intron 25.0%
!!! GTTTTCTGGTGTATAAATAA+TGG + chr5_2:44212564-44212583 Msa0771490:intron 25.0%
!!! CGGAAAAGTATTAGTTATTT+AGG + chr5_2:44212606-44212625 Msa0771490:intron 25.0%
! GTAAAATATAGATCCTACTT+AGG - chr5_2:44212670-44212689 None:intergenic 25.0%
!! CTCTTTTATCATAGGTTAAT+TGG + chr5_2:44212790-44212809 Msa0771490:intron 25.0%
! ACTATTATCATTCAAAGAGT+TGG - chr5_2:44213090-44213109 None:intergenic 25.0%
!! TCTGGTTCAATTAAGTTATT+TGG + chr5_2:44213245-44213264 Msa0771490:CDS 25.0%
!! TAAACTTTAGAACGTACCTT+TGG - chr5_2:44210172-44210191 None:intergenic 30.0%
AAAGTTTATTACTTCCCACA+AGG + chr5_2:44210184-44210203 Msa0771490:CDS 30.0%
CAATAACAGTGATTGTTTGA+GGG - chr5_2:44210240-44210259 None:intergenic 30.0%
TCAATAACAGTGATTGTTTG+AGG - chr5_2:44210241-44210260 None:intergenic 30.0%
CAAACAATCACTGTTATTGA+TGG + chr5_2:44210241-44210260 Msa0771490:CDS 30.0%
! TTCTTTTCCTTGTATCATCA+CGG + chr5_2:44210273-44210292 Msa0771490:CDS 30.0%
! GTTGTTGATAAAGAAGAAGA+CGG + chr5_2:44210385-44210404 Msa0771490:CDS 30.0%
! TTTTGTAAGAGTCTTTACGA+AGG - chr5_2:44210425-44210444 None:intergenic 30.0%
AAATCTGATTCTAATAACGG+CGG + chr5_2:44210442-44210461 Msa0771490:CDS 30.0%
!!! GTAGTAAAAAGGTGTCTTTT+GGG - chr5_2:44210607-44210626 None:intergenic 30.0%
!!! AGTAGTAAAAAGGTGTCTTT+TGG - chr5_2:44210608-44210627 None:intergenic 30.0%
! AAAAGACACCTTTTTACTAC+TGG + chr5_2:44210607-44210626 Msa0771490:CDS 30.0%
!!! AGTTTTTTCGTGTTCACAAA+CGG - chr5_2:44210640-44210659 None:intergenic 30.0%
AATCAAAGGCAGTGAATAAT+AGG + chr5_2:44210749-44210768 Msa0771490:CDS 30.0%
GAAGATTCATCAATCTTCAA+AGG - chr5_2:44211015-44211034 None:intergenic 30.0%
TGAGAAGAATTCGATGAATA+CGG + chr5_2:44211290-44211309 Msa0771490:intron 30.0%
! GTGGAGCAAATAAATTTTGT+TGG - chr5_2:44211508-44211527 None:intergenic 30.0%
TAAAGAGACAGAAAAATCTG+CGG - chr5_2:44211530-44211549 None:intergenic 30.0%
!! TTTGTGGTACTTTTAGGAAT+TGG - chr5_2:44211650-44211669 None:intergenic 30.0%
GCAAGAAAATTATGTGACTA+TGG + chr5_2:44211706-44211725 Msa0771490:intron 30.0%
TTGATACTGTAATCAGATGA+TGG + chr5_2:44211729-44211748 Msa0771490:intron 30.0%
! GATTTTTCGCAACTGTATTT+TGG - chr5_2:44211892-44211911 None:intergenic 30.0%
GAGTGAAGTTATAGATAACT+TGG + chr5_2:44212033-44212052 Msa0771490:intron 30.0%
TATTATCCGATCAACTAAGT+TGG + chr5_2:44212237-44212256 Msa0771490:intron 30.0%
!! TTCCCTTTTATTAGTGTTGA+AGG - chr5_2:44212300-44212319 None:intergenic 30.0%
! ATAAATAATGGAGAAGCTTC+AGG + chr5_2:44212576-44212595 Msa0771490:intron 30.0%
!!! GGTTTTTTTGTTGAGAGAAA+TGG - chr5_2:44212900-44212919 None:intergenic 30.0%
! AAAAAAACCTAGACTAGTTC+AGG + chr5_2:44212911-44212930 Msa0771490:CDS 30.0%
! ACTCTTGTTATCACTAAACA+AGG - chr5_2:44212943-44212962 None:intergenic 30.0%
AACTCTATCATAGGAATGAA+GGG - chr5_2:44212969-44212988 None:intergenic 30.0%
! TTTGGTTCAAAGATCTCATA+GGG - chr5_2:44212992-44213011 None:intergenic 30.0%
CATGATCATTTATCTCTATC+AGG + chr5_2:44213059-44213078 Msa0771490:CDS 30.0%
ATTAGTAGTTTGAACACCTT+TGG + chr5_2:44213176-44213195 Msa0771490:CDS 30.0%
TTAGTAGTTTGAACACCTTT+GGG + chr5_2:44213177-44213196 Msa0771490:CDS 30.0%
TGATACAAGGAAAAGAAGGA+CGG - chr5_2:44210270-44210289 None:intergenic 35.0%
GTGATGATACAAGGAAAAGA+AGG - chr5_2:44210274-44210293 None:intergenic 35.0%
TCTTCTTTATCAACAACCTC+AGG - chr5_2:44210382-44210401 None:intergenic 35.0%
TAAAGAAGAAGACGGTGATA+AGG + chr5_2:44210393-44210412 Msa0771490:CDS 35.0%
GAGAATTTAAGTCAAGCTTG+GGG - chr5_2:44210507-44210526 None:intergenic 35.0%
GGAGAATTTAAGTCAAGCTT+GGG - chr5_2:44210508-44210527 None:intergenic 35.0%
AGGAGAATTTAAGTCAAGCT+TGG - chr5_2:44210509-44210528 None:intergenic 35.0%
! AAATTCTCCTTTTCCATCTC+AGG + chr5_2:44210519-44210538 Msa0771490:CDS 35.0%
! GTTTTCATGAAAAACACTGC+CGG + chr5_2:44210679-44210698 Msa0771490:CDS 35.0%
GATTCCAACAACTATATCTC+CGG - chr5_2:44210701-44210720 None:intergenic 35.0%
! CAGTAAACTTCATGTTTCTG+CGG - chr5_2:44210723-44210742 None:intergenic 35.0%
AAAGGCAGTGAATAATAGGA+AGG + chr5_2:44210753-44210772 Msa0771490:CDS 35.0%
AAGGCAGTGAATAATAGGAA+GGG + chr5_2:44210754-44210773 Msa0771490:CDS 35.0%
TATATTACCCTAGGGCTAAT+TGG + chr5_2:44210911-44210930 Msa0771490:CDS 35.0%
TGTAGTAGATGCAAATGTTG+TGG + chr5_2:44210942-44210961 Msa0771490:CDS 35.0%
ATGATGCTATGAAGATTGGT+TGG + chr5_2:44210965-44210984 Msa0771490:CDS 35.0%
! TGATGCTATGAAGATTGGTT+GGG + chr5_2:44210966-44210985 Msa0771490:CDS 35.0%
!!! ATGACATTTTTTCAGCCTCA+AGG + chr5_2:44211045-44211064 Msa0771490:CDS 35.0%
ACATTAGAGATTGTTCCTTG+AGG - chr5_2:44211063-44211082 None:intergenic 35.0%
CAATACTATGAATCGATGCA+AGG - chr5_2:44211325-44211344 None:intergenic 35.0%
!! AAAAAAAGGTTTTCGGGAGT+GGG - chr5_2:44211397-44211416 None:intergenic 35.0%
!! AAAAAAAAGGTTTTCGGGAG+TGG - chr5_2:44211398-44211417 None:intergenic 35.0%
!!! ACCTTTTTAGAGCTTCAAAG+AGG + chr5_2:44211478-44211497 Msa0771490:intron 35.0%
!! ACCTCTTTGAAGCTCTAAAA+AGG - chr5_2:44211482-44211501 None:intergenic 35.0%
AACAAAGAGGACGAATTAAG+AGG - chr5_2:44211565-44211584 None:intergenic 35.0%
TGTGTGAAGATCAAACAAAG+AGG - chr5_2:44211578-44211597 None:intergenic 35.0%
TGATCTTCACACACTTATCT+CGG + chr5_2:44211585-44211604 Msa0771490:intron 35.0%
ACTAATTCCCCTGTAATTCA+TGG - chr5_2:44211622-44211641 None:intergenic 35.0%
!! TTCCTAAAAGTACCACAAAC+CGG + chr5_2:44211651-44211670 Msa0771490:intron 35.0%
GTGAATTGAAACTTGTGTAC+CGG - chr5_2:44211673-44211692 None:intergenic 35.0%
! GTTGAATAATGTTTTCGGCA+AGG - chr5_2:44211851-44211870 None:intergenic 35.0%
GGTATATATGTTTCAGCAAG+AGG + chr5_2:44212008-44212027 Msa0771490:intron 35.0%
GGGTTAAGTTATATGAGTGT+AGG + chr5_2:44212157-44212176 Msa0771490:intron 35.0%
TAACGACCAACTTAGTTGAT+CGG - chr5_2:44212246-44212265 None:intergenic 35.0%
TTGCAGTTGATCCATGATTA+TGG - chr5_2:44212427-44212446 None:intergenic 35.0%
AATCATATGAGGAGCAAGAA+GGG - chr5_2:44212505-44212524 None:intergenic 35.0%
ATCAACCACTCAATCATATG+AGG - chr5_2:44212516-44212535 None:intergenic 35.0%
! AAAGAGTGACAAGTGTTTTC+TGG + chr5_2:44212550-44212569 Msa0771490:intron 35.0%
ATTCGCTCTTATTCCTAAGT+AGG + chr5_2:44212654-44212673 Msa0771490:intron 35.0%
GAAATAAGTTCAACTTGCCA+AGG - chr5_2:44212855-44212874 None:intergenic 35.0%
ACTCTATCATAGGAATGAAG+GGG - chr5_2:44212968-44212987 None:intergenic 35.0%
GAACTCTATCATAGGAATGA+AGG - chr5_2:44212970-44212989 None:intergenic 35.0%
! GTTTGGTTCAAAGATCTCAT+AGG - chr5_2:44212993-44213012 None:intergenic 35.0%
GATCTTTGAACCAAACATTG+TGG + chr5_2:44212997-44213016 Msa0771490:CDS 35.0%
AACCAAACATTGTGGAATTG+CGG + chr5_2:44213005-44213024 Msa0771490:CDS 35.0%
TAGAGATAAATGATCATGCC+TGG - chr5_2:44213057-44213076 None:intergenic 35.0%
CTCAACAAAATCTATCCCAA+AGG - chr5_2:44213195-44213214 None:intergenic 35.0%
AACAGCAACACAAAAGAAGT+TGG + chr5_2:44213221-44213240 Msa0771490:CDS 35.0%
! AACACAAAAGAAGTTGGTTC+TGG + chr5_2:44213227-44213246 Msa0771490:CDS 35.0%
AATTTCATTGGATCCCTTAG+AGG - chr5_2:44213330-44213349 None:intergenic 35.0%
! GCCAGATTTTGGATTTAACG+CGG - chr5_2:44210120-44210139 None:intergenic 40.0%
AGTGATTGTTTGAGGGTTAG+GGG - chr5_2:44210233-44210252 None:intergenic 40.0%
CAGTGATTGTTTGAGGGTTA+GGG - chr5_2:44210234-44210253 None:intergenic 40.0%
ACAGTGATTGTTTGAGGGTT+AGG - chr5_2:44210235-44210254 None:intergenic 40.0%
AGTTCTTGTCCTTCAGTTAC+CGG - chr5_2:44210358-44210377 None:intergenic 40.0%
TCCGTTACTGATGTTCATGA+CGG + chr5_2:44210547-44210566 Msa0771490:CDS 40.0%
TCCGTCATGAACATCAGTAA+CGG - chr5_2:44210551-44210570 None:intergenic 40.0%
! AAGTTTGCTCATGTCTATCG+AGG + chr5_2:44210577-44210596 Msa0771490:CDS 40.0%
! AGTTTGCTCATGTCTATCGA+GGG + chr5_2:44210578-44210597 Msa0771490:CDS 40.0%
! GACACCTTTTTACTACTGGT+TGG + chr5_2:44210611-44210630 Msa0771490:CDS 40.0%
GAGTCCAACCAGTAGTAAAA+AGG - chr5_2:44210618-44210637 None:intergenic 40.0%
AACACGAAAAAACTCGTTGC+TGG + chr5_2:44210646-44210665 Msa0771490:CDS 40.0%
GTTTACTGCAGCTGAATCAA+AGG + chr5_2:44210735-44210754 Msa0771490:CDS 40.0%
AGGCAGTGAATAATAGGAAG+GGG + chr5_2:44210755-44210774 Msa0771490:CDS 40.0%
AATAATAGGAAGGGGGATGA+GGG + chr5_2:44210763-44210782 Msa0771490:CDS 40.0%
TGAGGGTAAAGAGAATGGTT+TGG + chr5_2:44210780-44210799 Msa0771490:CDS 40.0%
AATTGCACCAATTAGCCCTA+GGG - chr5_2:44210921-44210940 None:intergenic 40.0%
AAATTGCACCAATTAGCCCT+AGG - chr5_2:44210922-44210941 None:intergenic 40.0%
GTGGATGATGCTATGAAGAT+TGG + chr5_2:44210961-44210980 Msa0771490:CDS 40.0%
!!! CTTCAGGTGCTCTTCTTTTT+TGG + chr5_2:44211108-44211127 Msa0771490:intron 40.0%
ATTCGCATTTCGCGTTGTTA+CGG + chr5_2:44211250-44211269 Msa0771490:intron 40.0%
GAAGAATTCGATGAATACGG+AGG + chr5_2:44211293-44211312 Msa0771490:intron 40.0%
GCCAATCTCCATGAATTACA+GGG + chr5_2:44211611-44211630 Msa0771490:intron 40.0%
CCAATCTCCATGAATTACAG+GGG + chr5_2:44211612-44211631 Msa0771490:intron 40.0%
!!! TACCGGTTTGTGGTACTTTT+AGG - chr5_2:44211656-44211675 None:intergenic 40.0%
!! GTTGTGATTGTGAGCATTGA+AGG - chr5_2:44211923-44211942 None:intergenic 40.0%
AACAATGATCAAGGCTCTCA+AGG - chr5_2:44212080-44212099 None:intergenic 40.0%
ACTTGGAGCAACAATGATCA+AGG - chr5_2:44212089-44212108 None:intergenic 40.0%
CAATCATATGAGGAGCAAGA+AGG - chr5_2:44212506-44212525 None:intergenic 40.0%
TTGCTCCTCATATGATTGAG+TGG + chr5_2:44212508-44212527 Msa0771490:intron 40.0%
GAGAAGCTTCAGGTATCTTA+CGG + chr5_2:44212586-44212605 Msa0771490:intron 40.0%
! GTAGAATCCTGAACTAGTCT+AGG - chr5_2:44212921-44212940 None:intergenic 40.0%
CTCATAGGGAACTCTATCAT+AGG - chr5_2:44212978-44212997 None:intergenic 40.0%
AACCGCAATTCCACAATGTT+TGG - chr5_2:44213010-44213029 None:intergenic 40.0%
!! TGGAATTGCGGTTATTTTCC+AGG + chr5_2:44213017-44213036 Msa0771490:CDS 40.0%
!! AATTGCGGTTATTTTCCAGG+AGG + chr5_2:44213020-44213039 Msa0771490:CDS 40.0%
GAAAGTGATGTCCATCATTC+TGG + chr5_2:44213287-44213306 Msa0771490:CDS 40.0%
AAGGGAGAAGATTCCTCTAA+GGG + chr5_2:44213314-44213333 Msa0771490:CDS 40.0%
GCCGCGTTAAATCCAAAATC+TGG + chr5_2:44210116-44210135 Msa0771490:CDS 45.0%
AAAATCTGGCAGACATGTGC+CGG + chr5_2:44210130-44210149 Msa0771490:CDS 45.0%
TTACTTCCCACAAGGTCACT+TGG + chr5_2:44210192-44210211 Msa0771490:CDS 45.0%
CATGTTCCAAGTGACCTTGT+GGG - chr5_2:44210201-44210220 None:intergenic 45.0%
TTGTTTGAGGGTTAGGGGAA+GGG - chr5_2:44210228-44210247 None:intergenic 45.0%
ATTGTTTGAGGGTTAGGGGA+AGG - chr5_2:44210229-44210248 None:intergenic 45.0%
CAATTTGGCGAAGACCTCAT+CGG - chr5_2:44210326-44210345 None:intergenic 45.0%
TACCGGACTGAGTAGCAATT+TGG - chr5_2:44210341-44210360 None:intergenic 45.0%
TGAAGGACAAGAACTTCCTG+AGG + chr5_2:44210363-44210382 Msa0771490:CDS 45.0%
TCTGATTCTAATAACGGCGG+TGG + chr5_2:44210445-44210464 Msa0771490:CDS 45.0%
GGAAGATCAAGTCAGCACAA+AGG - chr5_2:44210486-44210505 None:intergenic 45.0%
GAGAGTTCCTGAGATGGAAA+AGG - chr5_2:44210529-44210548 None:intergenic 45.0%
! CTGATGTTCATGACGGAGTT+TGG + chr5_2:44210554-44210573 Msa0771490:CDS 45.0%
ACTGCCGGAGATATAGTTGT+TGG + chr5_2:44210694-44210713 Msa0771490:CDS 45.0%
GGCAGTGAATAATAGGAAGG+GGG + chr5_2:44210756-44210775 Msa0771490:CDS 45.0%
GAATAATAGGAAGGGGGATG+AGG + chr5_2:44210762-44210781 Msa0771490:CDS 45.0%
! GGGTAAAGAGAATGGTTTGG+AGG + chr5_2:44210783-44210802 Msa0771490:CDS 45.0%
! GAGAATGGTTTGGAGGTCAA+AGG + chr5_2:44210790-44210809 Msa0771490:CDS 45.0%
! AAAGGAGAGGGTTTTTCTCG+AGG + chr5_2:44210808-44210827 Msa0771490:CDS 45.0%
AAGGGGAATACTAACCGAGA+AGG + chr5_2:44210837-44210856 Msa0771490:CDS 45.0%
GGAGTTGGCGGAAAAGAATA+TGG + chr5_2:44210876-44210895 Msa0771490:CDS 45.0%
! ATGAAGATTGGTTGGGCATC+TGG + chr5_2:44210973-44210992 Msa0771490:CDS 45.0%
ATGTGTCCAGTGTCCCTAAT+TGG + chr5_2:44211079-44211098 Msa0771490:CDS 45.0%
TTCGCATTTCGCGTTGTTAC+GGG + chr5_2:44211251-44211270 Msa0771490:intron 45.0%
AGAGACAGAAAAATCTGCGG+TGG - chr5_2:44211527-44211546 None:intergenic 45.0%
CGCCAATCTCCATGAATTAC+AGG + chr5_2:44211610-44211629 Msa0771490:intron 45.0%
CCCCTGTAATTCATGGAGAT+TGG - chr5_2:44211615-44211634 None:intergenic 45.0%
!! GAAACTTGTGTACCGGTTTG+TGG - chr5_2:44211666-44211685 None:intergenic 45.0%
!! TTGTGTTGGTTGAGCTGAGA+CGG - chr5_2:44211827-44211846 None:intergenic 45.0%
! GTTTTCGGCAAGGATTGTGT+TGG - chr5_2:44211841-44211860 None:intergenic 45.0%
CATTGTTGCTGCTGCTATTG+TGG - chr5_2:44211989-44212008 None:intergenic 45.0%
CACAATAGCAGCAGCAACAA+TGG + chr5_2:44211987-44212006 Msa0771490:intron 45.0%
ATGTATAGCGTTGCGCCTTA+TGG + chr5_2:44212190-44212209 Msa0771490:intron 45.0%
TGTATAGCGTTGCGCCTTAT+GGG + chr5_2:44212191-44212210 Msa0771490:intron 45.0%
ATCAAAACCGCGTCAATCCT+TGG + chr5_2:44212835-44212854 Msa0771490:CDS 45.0%
TAGATGCACTGCAGGATTGT+GGG - chr5_2:44212877-44212896 None:intergenic 45.0%
AGAAATGGTAGATGCACTGC+AGG - chr5_2:44212885-44212904 None:intergenic 45.0%
!! TTATTTTCCAGGAGGCATGC+AGG + chr5_2:44213028-44213047 Msa0771490:CDS 45.0%
!! TATTTTCCAGGAGGCATGCA+GGG + chr5_2:44213029-44213048 Msa0771490:CDS 45.0%
TGTCCATCATTCTGGTAGCA+AGG + chr5_2:44213295-44213314 Msa0771490:CDS 45.0%
GTCCATCATTCTGGTAGCAA+GGG + chr5_2:44213296-44213315 Msa0771490:CDS 45.0%
CAAGGGAGAAGATTCCTCTA+AGG + chr5_2:44213313-44213332 Msa0771490:CDS 45.0%
!! AATTTAGTATATAAATATTA+GGG + chr5_2:44212137-44212156 Msa0771490:intron 5.0%
! GGCACATGTCTGCCAGATTT+TGG - chr5_2:44210131-44210150 None:intergenic 50.0%
TCCATCGGTGAACGTTCCAA+AGG + chr5_2:44210153-44210172 Msa0771490:CDS 50.0%
!! ACCTTTGGAACGTTCACCGA+TGG - chr5_2:44210157-44210176 None:intergenic 50.0%
GCATGTTCCAAGTGACCTTG+TGG - chr5_2:44210202-44210221 None:intergenic 50.0%
TCAACGGCCGTGATGATACA+AGG - chr5_2:44210283-44210302 None:intergenic 50.0%
CGCCAAATTGCTACTCAGTC+CGG + chr5_2:44210336-44210355 Msa0771490:CDS 50.0%
CTACTCAGTCCGGTAACTGA+AGG + chr5_2:44210346-44210365 Msa0771490:CDS 50.0%
ATCAAGTCAGCACAAAGGCG+AGG - chr5_2:44210481-44210500 None:intergenic 50.0%
! GTAACGGAGAGTTCCTGAGA+TGG - chr5_2:44210535-44210554 None:intergenic 50.0%
GGGGATGAGGGTAAAGAGAA+TGG + chr5_2:44210775-44210794 Msa0771490:CDS 50.0%
! TGGTTTGGAGGTCAAAGGAG+AGG + chr5_2:44210795-44210814 Msa0771490:CDS 50.0%
GGTTTGGAGGTCAAAGGAGA+GGG + chr5_2:44210796-44210815 Msa0771490:CDS 50.0%
! TTTTTCTCGAGGCGGGAGAA+GGG + chr5_2:44210819-44210838 Msa0771490:CDS 50.0%
GGGAATACTAACCGAGAAGG+CGG + chr5_2:44210840-44210859 Msa0771490:CDS 50.0%
TGCGTCAATAACCGCCTTCT+CGG - chr5_2:44210854-44210873 None:intergenic 50.0%
GGTTATTGACGCAGTGGAGT+TGG + chr5_2:44210861-44210880 Msa0771490:CDS 50.0%
TATTGACGCAGTGGAGTTGG+CGG + chr5_2:44210864-44210883 Msa0771490:CDS 50.0%
CCTGAAGCATGCGCCAATTA+GGG - chr5_2:44211095-44211114 None:intergenic 50.0%
ACCTGAAGCATGCGCCAATT+AGG - chr5_2:44211096-44211115 None:intergenic 50.0%
TTCGCGTTGTTACGGGTTTG+CGG + chr5_2:44211258-44211277 Msa0771490:intron 50.0%
CAACTTGCCAAGGATTGACG+CGG - chr5_2:44212845-44212864 None:intergenic 50.0%
GTAGATGCACTGCAGGATTG+TGG - chr5_2:44212878-44212897 None:intergenic 50.0%
GGCGACAAGTCGTTAGATGT+GGG - chr5_2:44213149-44213168 None:intergenic 50.0%
TGGCGACAAGTCGTTAGATG+TGG - chr5_2:44213150-44213169 None:intergenic 50.0%
CTCCCTTGCTACCAGAATGA+TGG - chr5_2:44213301-44213320 None:intergenic 50.0%
TTGGATTTAACGCGGCGTGC+CGG - chr5_2:44210112-44210131 None:intergenic 55.0%
TGGAACGTTCACCGATGGAC+CGG - chr5_2:44210152-44210171 None:intergenic 55.0%
AGGATCGGCCAGGAGATCAA+CGG - chr5_2:44210299-44210318 None:intergenic 55.0%
GACCTCATCGGTGTGAGGAT+CGG - chr5_2:44210314-44210333 None:intergenic 55.0%
! GGAGAGGGTTTTTCTCGAGG+CGG + chr5_2:44210811-44210830 Msa0771490:CDS 55.0%
! GAGAGGGTTTTTCTCGAGGC+GGG + chr5_2:44210812-44210831 Msa0771490:CDS 55.0%
! GTTTTTCTCGAGGCGGGAGA+AGG + chr5_2:44210818-44210837 Msa0771490:CDS 55.0%
! TTTTCTCGAGGCGGGAGAAG+GGG + chr5_2:44210820-44210839 Msa0771490:CDS 55.0%
GAAGGCGGTTATTGACGCAG+TGG + chr5_2:44210855-44210874 Msa0771490:CDS 55.0%
CATGCGCCAATTAGGGACAC+TGG - chr5_2:44211088-44211107 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTC+AGG + chr5_2:44211092-44211111 Msa0771490:CDS 55.0%
TGAGGAGCAAGAAGGGAGCA+TGG - chr5_2:44212498-44212517 None:intergenic 55.0%
ATTTAACGCGGCGTGCCGGA+GGG - chr5_2:44210108-44210127 None:intergenic 60.0%
! GCAGACATGTGCCGGTCCAT+CGG + chr5_2:44210138-44210157 Msa0771490:CDS 60.0%
TTGAGGGTTAGGGGAAGGGC+AGG - chr5_2:44210224-44210243 None:intergenic 60.0%
CATCACGGCCGTTGATCTCC+TGG + chr5_2:44210288-44210307 Msa0771490:CDS 60.0%
GCGAAGACCTCATCGGTGTG+AGG - chr5_2:44210319-44210338 None:intergenic 60.0%
GACAGACATGGTGGCCGGTG+GGG - chr5_2:44210086-44210105 None:intergenic 65.0%
GGACAGACATGGTGGCCGGT+GGG - chr5_2:44210087-44210106 None:intergenic 65.0%
GGAGGGACAGACATGGTGGC+CGG - chr5_2:44210091-44210110 None:intergenic 65.0%
GGCCACCATGTCTGTCCCTC+CGG + chr5_2:44210090-44210109 Msa0771490:CDS 65.0%
TGCCGGAGGGACAGACATGG+TGG - chr5_2:44210095-44210114 None:intergenic 65.0%
GATTTAACGCGGCGTGCCGG+AGG - chr5_2:44210109-44210128 None:intergenic 65.0%
CATCGGTGTGAGGATCGGCC+AGG - chr5_2:44210309-44210328 None:intergenic 65.0%
GGCCGATCCTCACACCGATG+AGG + chr5_2:44210309-44210328 Msa0771490:CDS 65.0%
GGGACAGACATGGTGGCCGG+TGG - chr5_2:44210088-44210107 None:intergenic 70.0%
GCGTGCCGGAGGGACAGACA+TGG - chr5_2:44210098-44210117 None:intergenic 70.0%
CTGGCTCCCTGCATGCCTCC+TGG - chr5_2:44213038-44213057 None:intergenic 70.0%
! CAGGAGGCATGCAGGGAGCC+AGG + chr5_2:44213036-44213055 Msa0771490:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 44210076 44213352 44210076 ID=Msa0771490;Name=Msa0771490
chr5_2 mRNA 44210076 44213352 44210076 ID=Msa0771490-mRNA-1;Parent=Msa0771490;Name=Msa0771490-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|528
chr5_2 exon 44210076 44211113 44210076 ID=Msa0771490-mRNA-1:exon:13508;Parent=Msa0771490-mRNA-1
chr5_2 exon 44212804 44213352 44212804 ID=Msa0771490-mRNA-1:exon:13509;Parent=Msa0771490-mRNA-1
chr5_2 CDS 44210076 44211113 44210076 ID=Msa0771490-mRNA-1:cds;Parent=Msa0771490-mRNA-1
chr5_2 CDS 44212804 44213352 44212804 ID=Msa0771490-mRNA-1:cds;Parent=Msa0771490-mRNA-1
Gene Sequence

>Msa0771490

ATGTCTTCCCCACCGGCCACCATGTCTGTCCCTCCGGCACGCCGCGTTAAATCCAAAATCTGGCAGACATGTGCCGGTCCATCGGTGAACGTTCCAAAGGTACGTTCTAAAGTTTATTACTTCCCACAAGGTCACTTGGAACATGCCTGCCCTTCCCCTAACCCTCAAACAATCACTGTTATTGATGGTTACCGTCCTTCTTTTCCTTGTATCATCACGGCCGTTGATCTCCTGGCCGATCCTCACACCGATGAGGTCTTCGCCAAATTGCTACTCAGTCCGGTAACTGAAGGACAAGAACTTCCTGAGGTTGTTGATAAAGAAGAAGACGGTGATAAGGTTGTTTCCTTCGTAAAGACTCTTACAAAATCTGATTCTAATAACGGCGGTGGATTCTCTGTACCTCGCCTTTGTGCTGACTTGATCTTCCCCAAGCTTGACTTAAATTCTCCTTTTCCATCTCAGGAACTCTCCGTTACTGATGTTCATGACGGAGTTTGGAAGTTTGCTCATGTCTATCGAGGGAGACCCAAAAGACACCTTTTTACTACTGGTTGGACTCCGTTTGTGAACACGAAAAAACTCGTTGCTGGTGATTCTATTGTTTTCATGAAAAACACTGCCGGAGATATAGTTGTTGGAATCCGCAGAAACATGAAGTTTACTGCAGCTGAATCAAAGGCAGTGAATAATAGGAAGGGGGATGAGGGTAAAGAGAATGGTTTGGAGGTCAAAGGAGAGGGTTTTTCTCGAGGCGGGAGAAGGGGAATACTAACCGAGAAGGCGGTTATTGACGCAGTGGAGTTGGCGGAAAAGAATATGGCATTTGAAGTTATATATTACCCTAGGGCTAATTGGTGCAATTTTGTAGTAGATGCAAATGTTGTGGATGATGCTATGAAGATTGGTTGGGCATCTGGAATGAGAGTGAAGCTTCCTTTGAAGATTGATGAATCTTCAAATTCAAAGATGACATTTTTTCAGCCTCAAGGAACAATCTCTAATGTGTCCAGTGTCCCTAATTGGCGCATGCTTCAGGTTAATTGGGATGAACTTGAAATCTTGCAAAATCAAAACCGCGTCAATCCTTGGCAAGTTGAACTTATTTCCCACAATCCTGCAGTGCATCTACCATTTCTCTCAACAAAAAAACCTAGACTAGTTCAGGATTCTACCTTGTTTAGTGATAACAAGAGTGACCCCTTCATTCCTATGATAGAGTTCCCTATGAGATCTTTGAACCAAACATTGTGGAATTGCGGTTATTTTCCAGGAGGCATGCAGGGAGCCAGGCATGATCATTTATCTCTATCAGGTTTTTCCAACTCTTTGAATGATAATAGTTATGGTTTCTTTGTGAACAATCTAAACATTGACAGTCCCACATCTAACGACTTGTCGCCAAATAACATTAGTAGTTTGAACACCTTTGGGATAGATTTTGTTGAGACTCACAACAGCAACACAAAAGAAGTTGGTTCTGGTTCAATTAAGTTATTTGGTAAGATCATACAGACTGTTGAAAGTGATGTCCATCATTCTGGTAGCAAGGGAGAAGATTCCTCTAAGGGATCCAATGAAATTTGA
Protein sequence

>Msa0771490

MSSPPATMSVPPARRVKSKIWQTCAGPSVNVPKVRSKVYYFPQGHLEHACPSPNPQTITVIDGYRPSFPCIITAVDLLADPHTDEVFAKLLLSPVTEGQELPEVVDKEEDGDKVVSFVKTLTKSDSNNGGGFSVPRLCADLIFPKLDLNSPFPSQELSVTDVHDGVWKFAHVYRGRPKRHLFTTGWTPFVNTKKLVAGDSIVFMKNTAGDIVVGIRRNMKFTAAESKAVNNRKGDEGKENGLEVKGEGFSRGGRRGILTEKAVIDAVELAEKNMAFEVIYYPRANWCNFVVDANVVDDAMKIGWASGMRVKLPLKIDESSNSKMTFFQPQGTISNVSSVPNWRMLQVNWDELEILQNQNRVNPWQVELISHNPAVHLPFLSTKKPRLVQDSTLFSDNKSDPFIPMIEFPMRSLNQTLWNCGYFPGGMQGARHDHLSLSGFSNSLNDNSYGFFVNNLNIDSPTSNDLSPNNISSLNTFGIDFVETHNSNTKEVGSGSIKLFGKIIQTVESDVHHSGSKGEDSSKGSNEI*