AlfalfaGEDB | Msa0777580

M. sativa cultivar zhongmu-4 / Msa0777580

KEGG: -
GO: GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0777580	sp\|Q9LVM0\|Y5830_ARATH	66.415	661	196	8	8	651	2	653	0.0	864

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0777580	G7K0V8	97.161	634	18	0	20	653	18	651	0.0	1263

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0777580	MtrunA17_Chr5g0432851	97.161	634	18	0	20	653	18	651	1263
Msa0777580	MtrunA17_Chr3g0108301	76.863	644	141	4	18	653	7	650	952
Msa0777580	MtrunA17_Chr3g0128551	61.726	614	224	6	46	653	27	635	754
Msa0777580	MtrunA17_Chr7g0245171	60.870	621	220	6	22	632	2	609	724
Msa0777580	MtrunA17_Chr5g0428541	58.481	619	216	10	24	631	1	589	670
Msa0777580	MtrunA17_Chr8g0387791	51.433	628	286	9	33	652	3	619	609
Msa0777580	MtrunA17_Chr4g0062911	53.760	625	255	11	51	653	30	642	605
Msa0777580	MtrunA17_Chr3g0126271	51.033	629	277	12	34	653	5	611	591
Msa0777580	MtrunA17_Chr5g0396941	49.515	618	263	11	33	642	9	585	559
Msa0777580	MtrunA17_Chr1g0159161	48.455	615	260	10	32	641	6	568	538

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0777580	AT5G58300.3	66.415	661	196	8	8	651	2	653	0.0	864
Msa0777580	AT5G58300.1	66.415	661	196	8	8	651	2	653	0.0	864
Msa0777580	AT5G58300.2	66.415	661	196	8	8	651	2	653	0.0	864
Msa0777580	AT3G08680.1	62.184	632	215	8	30	642	5	631	0.0	754
Msa0777580	AT3G08680.2	62.184	632	215	8	30	642	5	631	0.0	754
Msa0777580	AT5G05160.1	56.447	636	246	13	27	641	8	633	0.0	674
Msa0777580	AT2G26730.1	54.914	641	262	12	34	653	11	645	0.0	635
Msa0777580	AT4G23740.1	49.327	594	272	9	51	629	29	608	0.0	554
Msa0777580	AT4G23740.2	49.327	594	272	9	51	629	29	608	0.0	554
Msa0777580	AT1G48480.1	47.867	633	283	18	47	641	32	655	3.13e-178	521

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Find 190 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTTAGTTAGGAAATG+AGG	0.455502	5_2:+56664438	None:intergenic
AAACGACGGTTGTAGTCAAA+AGG	0.516466	5_2:-56661770	Msa0777580:CDS
AAAGCAATGATCAGTAAGCA+AGG	0.563198	5_2:+56664483	None:intergenic
AAAGCCAAAGGTCCTAGTGG+TGG	0.668375	5_2:-56661978	Msa0777580:CDS
AAAGGTCCTAGTGGTGGTGG+AGG	0.572909	5_2:-56661972	Msa0777580:CDS
AAATCAGATGTTTACAGTTT+TGG	0.267711	5_2:-56661002	Msa0777580:CDS
AAATGAAGCCATCGTTATCT+TGG	0.373819	5_2:+56661126	None:intergenic
AAGAAGTTGTGGTTGGCAAG+AGG	0.487187	5_2:-56661743	Msa0777580:CDS
AAGCAATGATCAGTAAGCAA+GGG	0.605597	5_2:+56664484	None:intergenic
AAGGGATAGATCCATTCAAA+TGG	0.539472	5_2:+56662261	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGAGGAATAAAGAATAGAG+TGG	+	chr5_2:56660547-56660566	None:intergenic	30.0%
TTTCTTGTAGCTGTTGTTAT+TGG	-	chr5_2:56660692-56660711	Msa0777580:CDS	30.0%
TTACCTTAAAGACAGCAAAA+AGG	+	chr5_2:56660906-56660925	None:intergenic	30.0%
TAAAACCAAACACACAATGT+GGG	+	chr5_2:56661071-56661090	None:intergenic	30.0%
TTAAAACCAAACACACAATG+TGG	+	chr5_2:56661072-56661091	None:intergenic	30.0%
TTGTACTTCAACATGTTTGT+TGG	-	chr5_2:56661109-56661128	Msa0777580:CDS	30.0%
TGTACTTCAACATGTTTGTT+GGG	-	chr5_2:56661110-56661129	Msa0777580:CDS	30.0%
ACCCTATATTAAGGCAAAAA+AGG	+	chr5_2:56661317-56661336	None:intergenic	30.0%
TTCTTCGTAACCCTATATTA+AGG	+	chr5_2:56661326-56661345	None:intergenic	30.0%
TAAAAGCTAAAAGCTTGCAT+TGG	-	chr5_2:56661351-56661370	Msa0777580:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	56660443	56664578	56660443	ID=Msa0777580;Name=Msa0777580
chr5_2	mRNA	56660443	56664578	56660443	ID=Msa0777580-mRNA-1;Parent=Msa0777580;Name=Msa0777580-mRNA-1;_AED=0.04;_eAED=0.04;_QI=484\|1\|1\|1\|0.33\|0\|4\|203\|653
chr5_2	exon	56660443	56660582	56660443	ID=Msa0777580-mRNA-1:exon:16758;Parent=Msa0777580-mRNA-1
chr5_2	exon	56660605	56661296	56660605	ID=Msa0777580-mRNA-1:exon:16757;Parent=Msa0777580-mRNA-1
chr5_2	exon	56661598	56662935	56661598	ID=Msa0777580-mRNA-1:exon:16756;Parent=Msa0777580-mRNA-1
chr5_2	exon	56664100	56664578	56664100	ID=Msa0777580-mRNA-1:exon:16755;Parent=Msa0777580-mRNA-1
chr5_2	five_prime_UTR	56664100	56664578	56664100	ID=Msa0777580-mRNA-1:five_prime_utr;Parent=Msa0777580-mRNA-1
chr5_2	five_prime_UTR	56662931	56662935	56662931	ID=Msa0777580-mRNA-1:five_prime_utr;Parent=Msa0777580-mRNA-1
chr5_2	CDS	56661598	56662930	56661598	ID=Msa0777580-mRNA-1:cds;Parent=Msa0777580-mRNA-1
chr5_2	CDS	56660668	56661296	56660668	ID=Msa0777580-mRNA-1:cds;Parent=Msa0777580-mRNA-1
chr5_2	three_prime_UTR	56660605	56660667	56660605	ID=Msa0777580-mRNA-1:three_prime_utr;Parent=Msa0777580-mRNA-1
chr5_2	three_prime_UTR	56660443	56660582	56660443	ID=Msa0777580-mRNA-1:three_prime_utr;Parent=Msa0777580-mRNA-1

Gene Sequence

>Msa0777580

ATGAAGGATGACAGAGATTACATTGAATTCAATTGTCAGTATCTTATACCATACACTAAAAAACTCCCCATGAAATTCTTCTCACCATTGCAAGCTTTTCTTTTTATCATTGTTATTCTTTCTCCTCTTGTTATTGCTGACCTGAATTCCGATAAACAAGCCCTTCTTGATTTTGCTTCTGCCGTCCCTCATCGTCGCAACCTTAAGTGGGACAATGCTACCTCAATATGTACATCTTGGATAGGCATCACTTGCAATCCAAACAGTACTCGTGTAGTTTCTGTCCGGCTCCCTGGAGTCGGACTAGTAGGCACCATTCCATCCAATACACTCGGCAAACTTGACTCACTTAAAATTATTAGCCTTCGCTCAAACCTACTAAGTGGAAGTATACCTCATGATATTGCTTCTCTTCCGTCTCTACAATATCTCTACCTTCAACATAATAACCTTTCTGGTGAATTACCAACATCATTGCCGTCACAACTCAGTGCACTTATTTTGTCTTACAATTCTTTCACAGGTACCATTCCGAAGACATTGCAAAACTTAACACAACTAACTCGATTGAGCCTCGAGAATAACTCCTTATCTGGACCGATACCTGATCTCCATGTAAACCTCAAACAATTGAATTTGAGCTACAACCATTTGAATGGATCTATCCCTTCATCTCTTCATAGTTTTTCAAGTTCTTCCTTTGAAGGGAACTCTCTCTTATGTGGATTGCCTCTAAAGCCGTGCTCCACAGTCCCTCCTCCATCGCCTCCTCCTACATTGACTCCTGTAATACACGACTCGAAAAATAAACTGAGTAAAGGAGCTATCATTGCAATTGCTGTTGGTGGAGCTGTTCTGTTATTTTTCGTAGCTCTTGTCATTGTTCTTTGTTGTTTAAAGAAAAAAGATAACGGTACCTCAAGAGTAGTTAAAGCCAAAGGTCCTAGTGGTGGTGGAGGAAGGACTGAAAAGCCGAAAGAAGAGTTTGGAAGTGGAGTGCAGGAATCCGAGAGAAACAAATTGGTTTTCTTTGAGGGATGCTCTTACAATTTCGATCTCGAGGATTTGTTGAGAGCTTCAGCTGAGGTCCTTGGAAAGGGTAGTTATGGTACTGCATATAAGGCTATATTGGAGGAGCAAACGACGGTTGTAGTCAAAAGGTTGAAAGAAGTTGTGGTTGGCAAGAGGGAATTTGAACAGCAGATGGAGATTGTTGGAAGCATTGGAAATCACCCAAATGTCGTTCCGCTTCGCGCTTATTATTATTCAAAGGATGAGAAGCTTTTAGTTTGTGACTATTTCCCAAATGGCAATCTATCTATACTCTTGCATGGTACTAGGACAGGCGGAAGAACTACTTTAGATTGGAACACACGAGTAAAAATCTCTCTCGGAATTGCCAGAGGAATTGCTCATTTACATTCAGTTGGTGGTCCAAGATTCACACATGGAAATGTGAAATCCTCCAATGTTCTCCTAAACCAAGATAACGATGGCTTCATTTCCGATTTCGGCCTGACACCACTCATGAACATTCCTGCGACCCCTTCTAGAACCATGGGCTACCGTGCTCCTGAGGTAATCGAGACTCGGAAGCACACGCATAAATCAGATGTTTACAGTTTTGGTGTCCTTCTTTTGGAAATGCTTACAGGTAAAGCGCCACAACAGTCTCCCGTACGCGATGACATGGTTGATCTCCCTAGATGGGTTCAGTCCGTTGTTAGGGAGGAGTGGACCGCCGAGGTTTTTGATGTGGAGCTGATGAGGTACCAAAACATTGAAGAAGAAATGGTGCAAATGTTGCAAATTGGCATGGCTTGTGTTGCGAAGGTTCCGGATATGAGGCCTAACATGGAGGAAGTAGTTAGAATGATTGAAGAAATTCGGCAATCTGACTCGGATAACCGGCCGTCTTCTGATGATAATAAATCCAAGGATTTAAATGTGCAAACTCCATGA

Protein sequence

>Msa0777580

MKDDRDYIEFNCQYLIPYTKKLPMKFFSPLQAFLFIIVILSPLVIADLNSDKQALLDFASAVPHRRNLKWDNATSICTSWIGITCNPNSTRVVSVRLPGVGLVGTIPSNTLGKLDSLKIISLRSNLLSGSIPHDIASLPSLQYLYLQHNNLSGELPTSLPSQLSALILSYNSFTGTIPKTLQNLTQLTRLSLENNSLSGPIPDLHVNLKQLNLSYNHLNGSIPSSLHSFSSSSFEGNSLLCGLPLKPCSTVPPPSPPPTLTPVIHDSKNKLSKGAIIAIAVGGAVLLFFVALVIVLCCLKKKDNGTSRVVKAKGPSGGGGRTEKPKEEFGSGVQESERNKLVFFEGCSYNFDLEDLLRASAEVLGKGSYGTAYKAILEEQTTVVVKRLKEVVVGKREFEQQMEIVGSIGNHPNVVPLRAYYYSKDEKLLVCDYFPNGNLSILLHGTRTGGRTTLDWNTRVKISLGIARGIAHLHSVGGPRFTHGNVKSSNVLLNQDNDGFISDFGLTPLMNIPATPSRTMGYRAPEVIETRKHTHKSDVYSFGVLLLEMLTGKAPQQSPVRDDMVDLPRWVQSVVREEWTAEVFDVELMRYQNIEEEMVQMLQIGMACVAKVPDMRPNMEEVVRMIEEIRQSDSDNRPSSDDNKSKDLNVQTP*