AlfalfaGEDB | Msa0778510

M. sativa cultivar zhongmu-4 / Msa0778510

KEGG: K04733
GO: GO:0001101 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0008150 GO:0009505 GO:0009506 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0030054 GO:0030312 GO:0031090 GO:0031224 GO:0031225 GO:0031226 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071944 GO:0097305 GO:0098588 GO:0098805 GO:1901700
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0778510	sp\|Q9LP77\|Y1848_ARATH	56.494	616	234	9	24	610	32	642	0.0	673

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0778510	G7K1N3	97.152	632	18	0	1	632	1	632	0.0	1261

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0778510	MtrunA17_Chr5g0434551	97.152	632	18	0	1	632	1	632	0.0	1261
Msa0778510	MtrunA17_Chr8g0393211	60.287	627	220	7	27	625	35	660	0.0	715
Msa0778510	MtrunA17_Chr6g0478621	57.771	637	224	10	24	618	22	655	0.0	686
Msa0778510	MtrunA17_Chr4g0062911	47.536	629	279	12	28	628	30	635	1.91e-166	490
Msa0778510	MtrunA17_Chr3g0108301	43.857	643	308	13	10	621	23	643	6.30e-166	489
Msa0778510	MtrunA17_Chr3g0128551	43.586	647	309	11	7	622	11	632	9.62e-166	488
Msa0778510	MtrunA17_Chr5g0432851	44.665	656	307	15	3	629	17	645	1.39e-165	488
Msa0778510	MtrunA17_Chr3g0126271	42.163	638	318	12	4	622	3	608	8.07e-158	467
Msa0778510	MtrunA17_Chr8g0387791	43.060	634	307	14	11	618	5	610	3.34e-155	461
Msa0778510	MtrunA17_Chr1g0209521	42.704	651	295	17	17	612	19	646	6.51e-149	446

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0778510	AT1G48480.1	56.494	616	234	9	24	610	32	642	0.0	673
Msa0778510	AT3G17840.1	55.769	624	240	9	23	617	29	645	0.0	650
Msa0778510	AT5G16590.1	53.084	616	263	8	8	610	11	613	0.0	631
Msa0778510	AT3G02880.1	51.339	635	272	8	1	614	1	619	0.0	615
Msa0778510	AT2G26730.1	46.463	622	297	10	11	610	12	619	2.77e-170	501
Msa0778510	AT3G08680.1	45.312	640	285	17	7	610	4	614	2.62e-164	485
Msa0778510	AT3G08680.2	45.312	640	285	17	7	610	4	614	2.62e-164	485
Msa0778510	AT5G58300.3	44.288	639	296	14	23	632	43	650	1.30e-157	468
Msa0778510	AT5G58300.1	44.288	639	296	14	23	632	43	650	1.30e-157	468
Msa0778510	AT5G58300.2	44.288	639	296	14	23	632	43	650	1.30e-157	468

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Find 182 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTTCTGCTGAAGTTT+TGG	0.218259	5_2:+59014880	Msa0778510:CDS
AAACCTCATTATTTCAATAT+TGG	0.267757	5_2:+59017180	Msa0778510:three_prime_UTR
AAACCTCTTCGGAATTTCTC+CGG	0.500931	5_2:-59014444	None:intergenic
AAAGCAGACGTCTATAGCTT+TGG	0.451446	5_2:+59016757	Msa0778510:CDS
AAAGGAGGAACATTAAGATC+AGG	0.541154	5_2:-59014389	None:intergenic
AACAACGAGGTCTTACATAA+TGG	0.415933	5_2:+59013528	Msa0778510:five_prime_UTR
AACAACTTATAAGGCAACAT+TGG	0.530845	5_2:+59014919	Msa0778510:CDS
AAGAGTACGTCCACCCACCG+TGG	0.742353	5_2:-59013970	None:intergenic
AAGGAGTAGACCTACCAAGA+TGG	0.552958	5_2:+59016839	Msa0778510:CDS
AATCCATTGTCCAAGACGAG+TGG	0.643270	5_2:+59016866	Msa0778510:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGAGAAAATTGAGAAGAAA+CGG	-	chr5_2:59013724-59013743	None:intergenic	30.0%
GATTCTTTGTGATGAATTTG+AGG	-	chr5_2:59013828-59013847	None:intergenic	30.0%
TTTGCAAAATCCATAGGAAT+TGG	-	chr5_2:59014170-59014189	None:intergenic	30.0%
TTTATACGGTTTGCAGAATT+TGG	+	chr5_2:59014253-59014272	Msa0778510:CDS	30.0%
TGTGAGTAGAAAGTTTAGTT+TGG	+	chr5_2:59014847-59014866	Msa0778510:CDS	30.0%
AACAACTTATAAGGCAACAT+TGG	+	chr5_2:59014919-59014938	Msa0778510:CDS	30.0%
TAAAATAATAGGCCCTAAGT+GGG	-	chr5_2:59015054-59015073	None:intergenic	30.0%
TGTGTATGATTATATGCCAA+TGG	+	chr5_2:59015093-59015112	Msa0778510:CDS	30.0%
GTGTATGATTATATGCCAAT+GGG	+	chr5_2:59015094-59015113	Msa0778510:CDS	30.0%
TTTGTGTTGTGATGCTAATA+AGG	+	chr5_2:59015217-59015236	Msa0778510:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	59013522	59017342	59013522	ID=Msa0778510;Name=Msa0778510
chr5_2	mRNA	59013522	59017342	59013522	ID=Msa0778510-mRNA-1;Parent=Msa0778510;Name=Msa0778510-mRNA-1;_AED=0.02;_eAED=0.02;_QI=339\|1\|1\|1\|1\|1\|2\|235\|632
chr5_2	exon	59013522	59015139	59013522	ID=Msa0778510-mRNA-1:exon:17375;Parent=Msa0778510-mRNA-1
chr5_2	exon	59016488	59017342	59016488	ID=Msa0778510-mRNA-1:exon:17376;Parent=Msa0778510-mRNA-1
chr5_2	five_prime_UTR	59013522	59013860	59013522	ID=Msa0778510-mRNA-1:five_prime_utr;Parent=Msa0778510-mRNA-1
chr5_2	CDS	59013861	59015139	59013861	ID=Msa0778510-mRNA-1:cds;Parent=Msa0778510-mRNA-1
chr5_2	CDS	59016488	59017107	59016488	ID=Msa0778510-mRNA-1:cds;Parent=Msa0778510-mRNA-1
chr5_2	three_prime_UTR	59017108	59017342	59017108	ID=Msa0778510-mRNA-1:three_prime_utr;Parent=Msa0778510-mRNA-1

Gene Sequence

>Msa0778510

ATGAAGCTCAAAAAAACCGTTCTGCTTTATTTCACAGCATGTTTAATAATCACCATTGTTTCCGGCGCCGATCTAGCTTCCGACAGAGCCAGTTTATTGACACTCCGCGCCACGGTGGGTGGACGTACTCTTCTATGGAATTCAACAGAAACAAACCCGTGTTTATGGACCGGAGTTATCTGCAATAACAAAAGAGTCACTGCCTTAAGGTTACCGGCGATGGGTCTCTCCGGTAACCTGCCTTCAGGTTTAGGTAATCTTACTGAATTGCAGACACTTTCTCTCCGGTATAACGCATTAACAGGACCAATTCCTATGGATTTTGCAAAACTGGTGTCACTTAGAAATCTTTATCTTCATAGTAACTTTTTCTCCGGTGAGGTGCCGGAGTTTTTATACGGTTTGCAGAATTTGGTGAGGTTGAATTTAGGTAAAAACAATTTCAGTGGTGAAATTTCTGAACATTTTAATAATCTTACTCGTTTGGATACATTGTTTTTGGAACAGAACATGTTTACTGGTTCTGTTCCTGATCTTAATGTTCCTCCTTTGCATCAGTTCAATGTATCGTTTAATAATTTAACCGGAGAAATTCCGAAGAGGTTTTCGCGCTTAAATGTAAGTGCTTTTTCTGGGAATTTGCTTTGTGGGAATCCTCTTCAAGTAGCTTGTCCTGGAAACAATGACAAAAATGGTTTATCTGGTGGAGCTATTGCTGGTATTGTGATTGGTTGTGTTTTTGGTTTGGTTTTGATTCTTGTTCTTTTGGTGTTGTGTTGTAGAAAAAGAAAAAAAAGTGATTCTGATAATGTAGCACGTGCCAAGAGTGTTGAAGGTGAGGTGTCACGTGAGAAAACTAGAGATTTTGAGAGTGGTGGTGGTGGTGATGGGAGTTATTCAGGTTTGGCTTCGGCTTCAACGACGGCTTCAACTTCGGTTTCAGCTTCTGGTGTGTCATTAGAGAAGAGTTTGATATTTATTGGGAATGTGAGTAGAAAGTTTAGTTTGGATGATTTGTTAAAAGCTTCTGCTGAAGTTTTGGGAAAAGGGACATTTGGAACAACTTATAAGGCAACATTGGAGATGGGAATAAGTGTAGCTGTGAAGAGGTTGAAGGATGTTACAGCAAGTGAAAGAGAATTCAGGGAGAAAATTGAGGAGGTTGGGAAATTGGTTCATGAGAAATTGGTCCCACTTAGGGCCTATTATTTTAGTAAAGATGAGAAGCTTGTTGTGTATGATTATATGCCAATGGGAAGCTTATCTGCACTCTTGCATGCAAATAATGGAGCTGGAAGGACTCCACTGAATTGGGAAACTAGGTCAACCATTGCCCTTGGTGCTGCTCAAGGGATTGCATACCTACACTCACAAGGTCCAACATCTTCCCATGGAAACATCAAGTCATCAAACATCCTTCTCACCAAATCATTTGAACCCCGCGTCTCGGATTTCGGCCTCGCTTACCTGGCTCTTCCAACCGCTACACCCAACCGAGTTTCCGGTTACCGTGCACCAGAGGTCACCGACGCACGCAAAGTATCCCAAAAAGCAGACGTCTATAGCTTTGGTATAATGCTTTTGGAGCTTCTTACCGGGAAGGCGCCTACTCATTCTTCGCTGAATGAAGAAGGAGTAGACCTACCAAGATGGGTCCAATCCATTGTCCAAGACGAGTGGAACACTGAGGTTTTCGACATGGAGCTTCTCAGGTACCAAAGTGTTGAGGAGGAAATGGTGAATCTTTTGCAGCTTGCTCTTGAATGTACCGCTCAATATCCTGATAAGAGACCTTCAATGGATGTGGTTGCAAGCAAGATTGAGAAAATTTGTCATCCTAGCTCAGAGAAAGAGGAAGAGAAGATTTATGATGATTTGTCTAAAGATGGAGATAATTGA

Protein sequence

>Msa0778510

MKLKKTVLLYFTACLIITIVSGADLASDRASLLTLRATVGGRTLLWNSTETNPCLWTGVICNNKRVTALRLPAMGLSGNLPSGLGNLTELQTLSLRYNALTGPIPMDFAKLVSLRNLYLHSNFFSGEVPEFLYGLQNLVRLNLGKNNFSGEISEHFNNLTRLDTLFLEQNMFTGSVPDLNVPPLHQFNVSFNNLTGEIPKRFSRLNVSAFSGNLLCGNPLQVACPGNNDKNGLSGGAIAGIVIGCVFGLVLILVLLVLCCRKRKKSDSDNVARAKSVEGEVSREKTRDFESGGGGDGSYSGLASASTTASTSVSASGVSLEKSLIFIGNVSRKFSLDDLLKASAEVLGKGTFGTTYKATLEMGISVAVKRLKDVTASEREFREKIEEVGKLVHEKLVPLRAYYFSKDEKLVVYDYMPMGSLSALLHANNGAGRTPLNWETRSTIALGAAQGIAYLHSQGPTSSHGNIKSSNILLTKSFEPRVSDFGLAYLALPTATPNRVSGYRAPEVTDARKVSQKADVYSFGIMLLELLTGKAPTHSSLNEEGVDLPRWVQSIVQDEWNTEVFDMELLRYQSVEEEMVNLLQLALECTAQYPDKRPSMDVVASKIEKICHPSSEKEEEKIYDDLSKDGDN*