Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0779100 | sp|F1B281|EBIII_PETHY | 55.440 | 193 | 80 | 2 | 2 | 194 | 3 | 189 | 1.18e-73 | 226 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0779100 | MtrunA17_Chr5g0435101 | 95.413 | 218 | 5 | 1 | 1 | 218 | 1 | 213 | 1.72e-152 | 421 |
| Msa0779100 | MtrunA17_Chr4g0044421 | 77.444 | 133 | 29 | 1 | 1 | 132 | 1 | 133 | 1.27e-72 | 218 |
| Msa0779100 | MtrunA17_Chr4g0037141 | 72.800 | 125 | 34 | 0 | 2 | 126 | 3 | 127 | 1.14e-69 | 214 |
| Msa0779100 | MtrunA17_Chr3g0131121 | 71.200 | 125 | 36 | 0 | 2 | 126 | 3 | 127 | 7.33e-69 | 213 |
| Msa0779100 | MtrunA17_Chr8g0385321 | 68.000 | 125 | 40 | 0 | 2 | 126 | 3 | 127 | 1.10e-64 | 202 |
| Msa0779100 | MtrunA17_Chr2g0281071 | 68.000 | 125 | 39 | 1 | 2 | 125 | 3 | 127 | 2.57e-62 | 197 |
| Msa0779100 | MtrunA17_Chr4g0048511 | 67.200 | 125 | 40 | 1 | 2 | 125 | 3 | 127 | 1.41e-61 | 196 |
| Msa0779100 | MtrunA17_Chr1g0193371 | 65.600 | 125 | 43 | 0 | 2 | 126 | 3 | 127 | 2.83e-61 | 195 |
| Msa0779100 | MtrunA17_Chr5g0410961 | 66.400 | 125 | 42 | 0 | 2 | 126 | 3 | 127 | 4.50e-61 | 194 |
| Msa0779100 | MtrunA17_Chr3g0115821 | 62.400 | 125 | 47 | 0 | 2 | 126 | 3 | 127 | 6.97e-61 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0779100 | AT4G38620.1 | 51.905 | 210 | 79 | 3 | 2 | 194 | 3 | 207 | 3.15e-72 | 221 |
| Msa0779100 | AT1G22640.1 | 73.016 | 126 | 34 | 0 | 2 | 127 | 3 | 128 | 2.38e-71 | 218 |
| Msa0779100 | AT2G16720.1 | 48.908 | 229 | 96 | 6 | 2 | 218 | 3 | 222 | 8.18e-71 | 217 |
| Msa0779100 | AT4G09460.1 | 72.000 | 125 | 35 | 0 | 2 | 126 | 3 | 127 | 1.95e-69 | 212 |
| Msa0779100 | AT4G34990.1 | 50.490 | 204 | 88 | 3 | 2 | 199 | 3 | 199 | 1.16e-67 | 209 |
| Msa0779100 | AT1G35515.1 | 69.355 | 124 | 38 | 0 | 2 | 125 | 3 | 126 | 7.67e-66 | 202 |
| Msa0779100 | AT4G28110.1 | 66.129 | 124 | 42 | 0 | 2 | 125 | 3 | 126 | 5.43e-64 | 200 |
| Msa0779100 | AT4G21440.1 | 60.274 | 146 | 51 | 1 | 2 | 147 | 3 | 141 | 7.48e-64 | 202 |
| Msa0779100 | AT5G16770.4 | 66.400 | 125 | 42 | 0 | 2 | 126 | 3 | 127 | 5.08e-63 | 199 |
| Msa0779100 | AT5G16770.3 | 66.400 | 125 | 42 | 0 | 2 | 126 | 3 | 127 | 5.08e-63 | 199 |
Find 49 sgRNAs with CRISPR-Local
Find 206 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATCAACAAAGGTGCT+TGG | 0.548391 | 5_2:-60281837 | Msa0779100:CDS |
| AAAGAAACATCATTATTAAC+GGG | 0.455198 | 5_2:+60280314 | None:intergenic |
| AACTAATTACCAGCAGCCTT+GGG | 0.489756 | 5_2:+60281745 | None:intergenic |
| AAGATTCCACGTTTGATATC+TGG | 0.258514 | 5_2:+60281443 | None:intergenic |
| AAGTTCATGGTGAAGGATGT+TGG | 0.511367 | 5_2:-60281777 | Msa0779100:CDS |
| AATAATCACAAACTACACAA+AGG | 0.672131 | 5_2:-60280434 | Msa0779100:CDS |
| ACAGTAGATACATGAGGACT+AGG | 0.609951 | 5_2:+60280407 | None:intergenic |
| ACCTATTGCCAAGGAGGGCA+TGG | 0.478073 | 5_2:+60281387 | None:intergenic |
| ACTTCATTATCTGTACGTCC+AGG | 0.545099 | 5_2:+60280515 | None:intergenic |
| AGGATTGTTATACCTAATTG+TGG | 0.497280 | 5_2:-60280209 | Msa0779100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GACAAAGAAAACATCAACAA+AGG | - | chr5_2:60280184-60280203 | Msa0779100:CDS | 30.0% | |
| TATATACAAGTTCATGGTGA+AGG | - | chr5_2:60280244-60280263 | Msa0779100:CDS | 30.0% | |
| AAGTTCAACGACTCTTTAAT+AGG | - | chr5_2:60280413-60280432 | Msa0779100:CDS | 30.0% | |
| CTCTCAGTCATGAATTATAT+AGG | - | chr5_2:60280804-60280823 | Msa0779100:intron | 30.0% | |
| GACAAACATATTTCCTTATC+AGG | + | chr5_2:60281353-60281372 | None:intergenic | 30.0% | |
| ATAACAATCCTATTTGGAGA+AGG | + | chr5_2:60281810-60281829 | None:intergenic | 30.0% | |
| AGGATTGTTATACCTAATTG+TGG | - | chr5_2:60281819-60281838 | Msa0779100:CDS | 30.0% | |
| GGATTGTTATACCTAATTGT+GGG | - | chr5_2:60281820-60281839 | Msa0779100:CDS | 30.0% | |
| GACAAAGAAAACATCAACAA+AGG | - | chr5_2:60280184-60280203 | Msa0779100:CDS | 30.0% | |
| TATATACAAGTTCATGGTGA+AGG | - | chr5_2:60280244-60280263 | Msa0779100:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 60280166 | 60281884 | 60280166 | ID=Msa0779100;Name=Msa0779100 |
| chr5_2 | mRNA | 60280166 | 60281884 | 60280166 | ID=Msa0779100-mRNA-1;Parent=Msa0779100;Name=Msa0779100-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|1|1|3|0|218 |
| chr5_2 | exon | 60281755 | 60281884 | 60281755 | ID=Msa0779100-mRNA-1:exon:17620;Parent=Msa0779100-mRNA-1 |
| chr5_2 | exon | 60281389 | 60281518 | 60281389 | ID=Msa0779100-mRNA-1:exon:17619;Parent=Msa0779100-mRNA-1 |
| chr5_2 | exon | 60280166 | 60280562 | 60280166 | ID=Msa0779100-mRNA-1:exon:17618;Parent=Msa0779100-mRNA-1 |
| chr5_2 | CDS | 60281755 | 60281884 | 60281755 | ID=Msa0779100-mRNA-1:cds;Parent=Msa0779100-mRNA-1 |
| chr5_2 | CDS | 60281389 | 60281518 | 60281389 | ID=Msa0779100-mRNA-1:cds;Parent=Msa0779100-mRNA-1 |
| chr5_2 | CDS | 60280166 | 60280562 | 60280166 | ID=Msa0779100-mRNA-1:cds;Parent=Msa0779100-mRNA-1 |
| Gene Sequence |
>Msa0779100 ATGAGAAAGCCTTGTTGTGACAAAGAAAACATCAACAAAGGTGCTTGGTCTAAGCAAGAAGATCAAAAGCTCATTGATTATATACAAGTTCATGGTGAAGGATGTTGGGGCTCCATTCCCAAGGCTGCTGGGTTGCATCGTTGTGGCAAAAGCTGTAGGCTAAGATGGTTAAACTACTTACGACCAGATATCAAACGTGGAATCTTTGCACAAGATGAAGAAGACCTCATCATCAAACTCCATGCCCTCCTTGGCAATAGGTGGGCACTCATAGCTGGAAGGTTGCCTGGACGTACAGATAATGAAGTGAAAAACTATTGGAATTCTCATATTCGTAGAAAGCTAATAAAAATGGGAATTGATCCAAATAATCACAAACTACACAAAGGATTTCCTAGTCCTCATGTATCTACTGTGGGCACATCATCATGTGTTGAGTCCATGAACAAAGAGAATAATAATCTCTCCATCAAGTCGTCTGATGCACCCGTTAATAATGATGTTTCTTTCGTAAAGAAAGATACTACATCAATAATTAATTCTTCATCATCTTTGAATCTTGACTTAACCATTGCTTTACCTTCTCCAAATAGGATTGTTATACCTAATTGTGGGTCTCCTAAGACTAGGGATATGGATATTGATCTTAATTGTTAA |
| Protein sequence |
>Msa0779100 MRKPCCDKENINKGAWSKQEDQKLIDYIQVHGEGCWGSIPKAAGLHRCGKSCRLRWLNYLRPDIKRGIFAQDEEDLIIKLHALLGNRWALIAGRLPGRTDNEVKNYWNSHIRRKLIKMGIDPNNHKLHKGFPSPHVSTVGTSSCVESMNKENNNLSIKSSDAPVNNDVSFVKKDTTSIINSSSSLNLDLTIALPSPNRIVIPNCGSPKTRDMDIDLNC* |
