AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0779220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0779220 MtrunA17_Chr3g0077411 65.583 523 135 9 1 478 1 523 0.0 643
Msa0779220 MtrunA17_Chr3g0077361 61.133 512 141 6 1 478 20 507 0.0 567
Msa0779220 MtrunA17_Chr3g0077461 59.611 463 142 10 36 470 22 467 2.31e-178 508
Msa0779220 MtrunA17_Chr3g0077461 46.835 237 91 5 16 220 232 465 2.06e-58 199
Msa0779220 MtrunA17_Chr4g0009491 58.372 430 135 5 35 430 11 430 5.59e-161 462
Msa0779220 MtrunA17_Chr4g0009491 49.289 211 74 3 250 449 11 199 3.50e-52 182
Msa0779220 MtrunA17_Chr4g0009491 46.602 206 65 2 16 176 220 425 6.51e-49 173
Msa0779220 MtrunA17_Chr3g0077381 55.782 441 121 7 35 430 34 445 1.24e-145 424
Msa0779220 MtrunA17_Chr3g0077381 47.739 199 66 4 16 176 242 440 1.40e-48 172
Msa0779220 MtrunA17_Chr3g0077381 46.781 233 67 5 250 469 34 222 1.54e-48 172
Msa0779220 MtrunA17_Chr5g0429931 66.527 239 69 1 251 478 10 248 1.95e-107 319
Msa0779220 MtrunA17_Chr5g0429931 50.216 231 69 3 36 221 10 239 2.28e-64 208
Msa0779220 MtrunA17_Chr3g0077451 62.241 241 69 3 250 478 10 240 7.85e-101 302
Msa0779220 MtrunA17_Chr3g0077451 50.655 229 76 4 35 227 10 237 2.47e-64 208
Msa0779220 MtrunA17_Chr3g0077431 60.664 211 61 3 280 478 1 201 1.99e-83 256
Msa0779220 MtrunA17_Chr3g0077431 46.231 199 70 4 65 227 1 198 1.30e-44 155
Msa0779220 MtrunA17_Chr3g0077421 60.000 210 61 3 280 476 1 200 6.53e-79 244
Msa0779220 MtrunA17_Chr3g0077421 45.361 194 68 4 65 221 1 193 2.39e-40 144
Msa0779220 MtrunA17_Chr3g0077441 60.000 210 61 3 280 476 1 200 6.53e-79 244
Msa0779220 MtrunA17_Chr3g0077441 45.361 194 68 4 65 221 1 193 2.39e-40 144
Msa0779220 MtrunA17_Chr5g0421921 43.860 114 63 1 365 478 2 114 8.22e-24 95.9
Msa0779220 MtrunA17_Chr5g0421731 43.860 114 63 1 365 478 2 114 8.22e-24 95.9
Msa0779220 MtrunA17_Chr5g0421851 43.860 114 63 1 365 478 77 189 1.33e-22 95.1
Msa0779220 MtrunA17_Chr1g0162441 41.414 99 54 1 21 115 7 105 5.39e-21 95.1
Msa0779220 MtrunA17_Chr1g0162441 38.889 90 55 0 236 325 7 96 1.15e-18 88.2
Msa0779220 MtrunA17_Chr1g0162441 35.115 131 76 3 351 475 268 395 2.67e-12 68.6
Msa0779220 MtrunA17_Chr1g0162411 38.542 96 59 0 236 331 7 102 2.07e-18 87.4
Msa0779220 MtrunA17_Chr1g0162411 39.394 99 54 2 21 115 7 103 2.82e-16 80.9
Msa0779220 MtrunA17_Chr4g0033961 35.294 119 66 2 9 123 1 112 1.13e-17 85.1
Msa0779220 MtrunA17_Chr4g0033961 36.047 86 55 0 233 318 3 88 2.75e-14 74.7
Msa0779220 MtrunA17_Chr4g0033961 35.172 145 71 5 337 475 264 391 1.63e-12 69.3
Msa0779220 MtrunA17_Chr3g0131891 25.175 286 166 8 235 472 18 303 9.73e-17 81.3
Msa0779220 MtrunA17_Chr3g0131891 29.825 114 76 1 8 117 6 119 1.94e-14 74.3
Msa0779220 MtrunA17_Chr5g0419201 34.343 99 61 1 21 115 16 114 1.17e-15 75.5
Msa0779220 MtrunA17_Chr5g0419201 30.088 113 68 1 222 323 2 114 2.90e-11 62.8
Msa0779220 MtrunA17_Chr7g0233561 28.477 151 100 2 7 149 4 154 3.99e-15 76.3
Msa0779220 MtrunA17_Chr1g0162421 45.455 55 30 0 35 89 7 61 9.29e-15 68.9
Msa0779220 MtrunA17_Chr1g0162421 44.828 58 32 0 250 307 7 64 5.45e-14 66.6
Msa0779220 MtrunA17_Chr7g0233521 26.296 270 142 9 252 472 2 263 4.53e-14 72.4
Msa0779220 MtrunA17_Chr7g0233521 30.476 105 65 2 37 133 2 106 1.46e-12 68.2
Msa0779220 MtrunA17_Chr3g0103151 38.000 100 56 2 20 115 11 108 7.33e-14 69.3
Msa0779220 MtrunA17_Chr3g0103171 22.989 261 161 5 251 471 5 265 1.99e-13 70.5
Msa0779220 MtrunA17_Chr3g0103171 40.476 84 46 1 36 115 5 88 4.92e-13 69.3
Msa0779220 MtrunA17_Chr3g0103181 39.000 100 55 2 20 115 11 108 2.35e-13 70.5
Msa0779220 MtrunA17_Chr3g0103191 38.384 99 55 2 21 115 12 108 3.09e-13 69.7
Msa0779220 MtrunA17_Chr7g0233571 30.693 101 66 1 20 116 9 109 5.74e-13 68.2
Msa0779220 MtrunA17_Chr7g0233571 27.536 138 89 1 235 361 9 146 6.31e-12 65.1
Msa0779220 MtrunA17_Chr7g0233541 32.000 100 62 2 37 130 2 101 9.20e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0779220 AT3G18990.1 39.394 99 56 1 21 115 6 104 3.94e-18 85.9
Msa0779220 AT3G18990.1 36.620 142 78 5 341 476 205 340 1.01e-12 69.7
Msa0779220 AT3G18990.1 34.444 90 59 0 236 325 6 95 1.49e-12 69.3
Msa0779220 AT3G18990.2 39.394 99 56 1 21 115 6 104 7.16e-18 85.5
Msa0779220 AT3G18990.2 34.444 90 59 0 236 325 6 95 2.17e-12 68.9
Msa0779220 AT3G18990.2 35.971 139 77 5 341 473 205 337 1.25e-11 66.6
Msa0779220 AT1G49475.1 36.800 125 72 3 5 125 20 141 2.59e-16 77.8
Msa0779220 AT4G01580.1 35.185 108 66 1 14 117 23 130 1.90e-15 75.1
Msa0779220 AT4G01580.1 31.405 121 70 2 221 328 13 133 4.75e-12 65.5
Msa0779220 AT3G18960.2 34.259 108 67 1 14 117 23 130 6.26e-15 73.6
Msa0779220 AT3G18960.2 30.252 119 70 2 220 325 12 130 6.47e-11 62.0
Msa0779220 AT3G18960.1 34.259 108 67 1 14 117 23 130 1.27e-14 73.2
Msa0779220 AT3G18960.1 29.508 122 73 2 220 328 12 133 5.16e-11 62.8
Msa0779220 AT1G49480.3 34.848 132 76 4 351 476 98 225 1.75e-12 67.4
Msa0779220 AT1G49480.1 34.848 132 76 4 351 476 98 225 1.75e-12 67.4
Msa0779220 AT1G49480.2 34.848 132 76 4 351 476 98 225 1.75e-12 67.4

Find 102 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGACTTTATAACAATATT+TGG 0.103105 5_2:+60392087 Msa0779220:CDS
ACTGCTTTCCTTGAGAATTT+TGG 0.150373 5_2:-60391256 None:intergenic
TGTCTAAATGTGCTTCTAAA+AGG 0.179114 5_2:+60392439 Msa0779220:CDS
CATTTGCATTGCTGATGTTT+TGG 0.183136 5_2:-60392732 None:intergenic
ACTGCTCTCCATGAGAATTT+TGG 0.185651 5_2:-60392682 None:intergenic
GACGAGTTTATAACAAGATT+TGG 0.231276 5_2:+60390778 Msa0779220:CDS
GGATGGTCTCATTTGGAAAA+TGG 0.260394 5_2:+60392146 Msa0779220:CDS
GGTGAAATTTACAATTTCTT+TGG 0.278329 5_2:-60391967 None:intergenic
TATGGCCTTCTTAAAATGTT+TGG 0.280661 5_2:-60391925 None:intergenic
AGTGAAGCATACTATTTCTT+TGG 0.281017 5_2:-60389985 None:intergenic
TACAATTTCTTTGGCATGAA+TGG 0.281456 5_2:-60391958 None:intergenic
AATATTGTTATAAAGTCTTC+TGG 0.286515 5_2:-60392084 None:intergenic
ATTTCCTATCCATTCAAAAC+TGG 0.287220 5_2:+60391012 Msa0779220:CDS
GGACTATGACACTCTTTGTT+TGG 0.315553 5_2:-60392360 None:intergenic
TTCAAAACTCTAAGTACTAA+TGG 0.332671 5_2:+60392333 Msa0779220:CDS
AAGTGAGAGTCAAGAGAAAA+AGG 0.347170 5_2:+60392407 Msa0779220:CDS
AAGACTTTATAACAATATTT+GGG 0.361172 5_2:+60392088 Msa0779220:CDS
ACGAGTTTATAACAAGATTT+GGG 0.367534 5_2:+60390779 Msa0779220:CDS
TTCAAAACTGGGAAGACTAA+TGG 0.374313 5_2:+60391024 Msa0779220:CDS
AAATCAATGCGAATAACGTT+TGG 0.379436 5_2:+60391314 Msa0779220:CDS
CTGTTCCGGATGGTCTCATT+TGG 0.388671 5_2:+60392139 Msa0779220:CDS
TGGAGCATGAACAGAACATT+TGG 0.389188 5_2:-60391236 None:intergenic
TGGAGCTTGATAGGACTATT+CGG 0.392252 5_2:-60392662 None:intergenic
TACTATTTCTTTGGTATGCA+TGG 0.398817 5_2:-60389976 None:intergenic
TGCAGGCGGAGAAGGAAGTT+AGG 0.415021 5_2:+60391898 Msa0779220:intron
ACTCTCACTTGTTTCGACCT+TGG 0.417847 5_2:-60392394 None:intergenic
ATGAATGGGTGACGGAAGTA+TGG 0.419901 5_2:-60391943 None:intergenic
TATGTAGATTTCAAGTATGA+AGG 0.431237 5_2:+60390943 Msa0779220:intron
TGTTGCTACAGTTACTGTTC+CGG 0.431782 5_2:+60392125 Msa0779220:CDS
AATCTTGTTATAAACTCGTC+TGG 0.437746 5_2:-60390775 None:intergenic
ACAATTTCTTTGGCATGAAT+GGG 0.438843 5_2:-60391957 None:intergenic
ATTTCTTTGGTATGCATGGG+AGG 0.447169 5_2:-60389972 None:intergenic
GACCACTACTCTATCTGTTA+TGG 0.450681 5_2:+60390916 Msa0779220:CDS
TCCTTCTCCGGCGCCGGTGA+CGG 0.453830 5_2:-60389918 None:intergenic
GTCTAAATGTGCTTCTAAAA+GGG 0.464166 5_2:+60392440 Msa0779220:CDS
TCCGTCACCGGCGCCGGAGA+AGG 0.467872 5_2:+60389917 Msa0779220:CDS
TGCACTTTGAACTAAAATGA+AGG 0.469656 5_2:-60392497 None:intergenic
TTCTTTGGTATGCATGGGAG+GGG 0.472658 5_2:-60389970 None:intergenic
TTGGAATCCTTCTCCGGCGC+CGG 0.476077 5_2:-60389924 None:intergenic
GAGAAGATTTGTTCCGTCAC+CGG 0.477470 5_2:+60389905 Msa0779220:CDS
ACAAAGAGTGTCATAGTCCT+AGG 0.483569 5_2:+60392364 Msa0779220:CDS
GTATGAGTTTGAGCTTATCA+AGG 0.487262 5_2:+60391382 Msa0779220:CDS
AAATGTTTGGAATCCTTCTC+CGG 0.487728 5_2:-60389930 None:intergenic
ACTATTTCTTTGGTATGCAT+GGG 0.496981 5_2:-60389975 None:intergenic
TATTGAATTGCAGGCGGAGA+AGG 0.498005 5_2:+60391890 Msa0779220:intron
ATATCGACAGTCAAAGGATT+GGG 0.499850 5_2:-60391079 None:intergenic
CAAAACATCAGCAATGCAAA+TGG 0.502620 5_2:+60392733 Msa0779220:CDS
ATTTGTTCCGTCACCGGCGC+CGG 0.504505 5_2:+60389911 Msa0779220:CDS
AAATACTATTCTATAGGATA+CGG 0.506013 5_2:+60392225 Msa0779220:CDS
ATCAAGCTCCAAAATTCTCA+TGG 0.511554 5_2:+60392674 Msa0779220:CDS
TTGGATTCTTTAGATTTGCA+AGG 0.513403 5_2:+60391333 Msa0779220:CDS
TGATGGTTGTGAATGGCATA+TGG 0.515259 5_2:+60390837 Msa0779220:CDS
AGTGTCATAGTCCTAGGAAA+AGG 0.516090 5_2:+60392370 Msa0779220:CDS
CATATCGACAGTCAAAGGAT+TGG 0.519552 5_2:-60391080 None:intergenic
AAGGATTGGGAACGAAAAGA+AGG 0.522583 5_2:-60391066 None:intergenic
TACTCTGCAGCAAATTCAGC+AGG 0.524148 5_2:-60391206 None:intergenic
GCAGGCGGAGAAGGAAGTTA+GGG 0.528822 5_2:+60391899 Msa0779220:intron
ACAAAACAATGCAGCCTCGC+CGG 0.533798 5_2:+60389883 None:intergenic
TATGAGTTTGAGCTTATCAA+GGG 0.535963 5_2:+60391383 Msa0779220:CDS
CTGTTCCTGATGGTTGTGAA+TGG 0.538019 5_2:+60390830 Msa0779220:CDS
AGGGATTTATGAAATTCGCT+AGG 0.541517 5_2:+60392759 Msa0779220:CDS
CACCGCATATCGACAGTCAA+AGG 0.544452 5_2:-60391085 None:intergenic
ATCAGCAATGCAAATGGTGA+AGG 0.544724 5_2:+60392739 Msa0779220:CDS
CCTCACCGCATATCTTCCCT+TGG 0.546419 5_2:-60392520 None:intergenic
TTTCCTATCCATTCAAAACT+GGG 0.546790 5_2:+60391013 Msa0779220:CDS
AGGGAAAACAATCTATCAGA+TGG 0.548736 5_2:+60391353 Msa0779220:CDS
ATGTAGATTTCAAGTATGAA+GGG 0.551054 5_2:+60390944 Msa0779220:intron
TTTCTTTGGTATGCATGGGA+GGG 0.553788 5_2:-60389971 None:intergenic
ATATGCCATTCACAACCATC+AGG 0.554864 5_2:-60390835 None:intergenic
GTGACGGAACAAATCTTCTC+CGG 0.557768 5_2:-60389902 None:intergenic
TCTTTGGTATGCATGGGAGG+GGG 0.563298 5_2:-60389969 None:intergenic
TTCATGCTCCAAAATTCTCA+AGG 0.564002 5_2:+60391248 Msa0779220:CDS
TGGATTCTTTAGATTTGCAA+GGG 0.565236 5_2:+60391334 Msa0779220:CDS
ATGTGCTTCTAAAAGGGCTG+AGG 0.566872 5_2:+60392446 Msa0779220:CDS
TATTGTGTATTGAATAAAAG+CGG 0.577376 5_2:+60391287 Msa0779220:CDS
TTTAGTTCAAAGTGCACCAA+GGG 0.580906 5_2:+60392504 Msa0779220:CDS
AAATTCTCATGGAGAGCAGT+GGG 0.586451 5_2:+60392685 Msa0779220:CDS
TGAGCTAATCAAGAAGAAGC+CGG 0.587226 5_2:+60392817 Msa0779220:CDS
GGGATTTATGAAATTCGCTA+GGG 0.596283 5_2:+60392760 Msa0779220:CDS
GCTACGATTACTGTTCCTGA+TGG 0.596760 5_2:+60390820 Msa0779220:CDS
TGTGTATTGAATAAAAGCGG+TGG 0.597510 5_2:+60391290 Msa0779220:CDS
AAAATTCTCAAGGAAAGCAG+TGG 0.599450 5_2:+60391258 Msa0779220:CDS
TCAGCAATGCAAATGGTGAA+GGG 0.605504 5_2:+60392740 Msa0779220:CDS
AAAATTCTCATGGAGAGCAG+TGG 0.606270 5_2:+60392684 Msa0779220:CDS
TCTTTGGCATGAATGGGTGA+CGG 0.612098 5_2:-60391951 None:intergenic
AAAATGGAGTTGAAGAAATG+TGG 0.612838 5_2:+60392162 Msa0779220:CDS
AGGATTGGGAACGAAAAGAA+GGG 0.614754 5_2:-60391065 None:intergenic
ATCCTTTGACTGTCGATATG+CGG 0.632326 5_2:+60391083 Msa0779220:CDS
GCTACAGTTACTGTTCCGGA+TGG 0.632577 5_2:+60392129 Msa0779220:CDS
ATAGAGTAGTGGTCTGCGAA+CGG 0.635190 5_2:-60390907 None:intergenic
AAATTCTCAAGGAAAGCAGT+GGG 0.637805 5_2:+60391259 Msa0779220:CDS
TAGTCCTAGGAAAAGGTCCA+AGG 0.641569 5_2:+60392377 Msa0779220:CDS
ACAAGTTACAGCTTCTCACA+CGG 0.646742 5_2:+60391424 Msa0779220:CDS
GGAGTTGAAGAAATGTGGCA+AGG 0.665180 5_2:+60392167 Msa0779220:CDS
AACCATAACAGATAGAGTAG+TGG 0.673476 5_2:-60390918 None:intergenic
GTGCACCAAGGGAAGATATG+CGG 0.688590 5_2:+60392515 Msa0779220:CDS
GGAACAAATCTTCTCCGGCG+AGG 0.698114 5_2:-60389897 None:intergenic
ATGAGTTTGAGCTTATCAAG+GGG 0.700277 5_2:+60391384 Msa0779220:CDS
CATATGGAAGTAAAGAAACG+TGG 0.701477 5_2:+60390853 Msa0779220:CDS
AAAAGTCACAATGTTCCGCG+TGG 0.739610 5_2:+60392847 Msa0779220:CDS
GTAACTTGTAACACAACACG+CGG 0.742805 5_2:-60391410 None:intergenic
AATCACTATACTCAACCACG+CGG 0.838428 5_2:-60392862 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTTTACTATACATTATT+TGG + chr5_2:60391109-60391128 Msa0779220:intron 15.0%
!!! ATGTTTGAGTTATATATTTA+TGG + chr5_2:60391143-60391162 Msa0779220:intron 15.0%
!! AAGACTTTATAACAATATTT+GGG + chr5_2:60392088-60392107 Msa0779220:CDS 15.0%
!!! ATAGAATAGTATTTTGAAAA+CGG - chr5_2:60392219-60392238 None:intergenic 15.0%
!!! TTTTCAAAATACTATTCTAT+AGG + chr5_2:60392219-60392238 Msa0779220:CDS 15.0%
!!! AATGGTTTTCATAAAATGTT+TGG - chr5_2:60389946-60389965 None:intergenic 20.0%
!! TAGTTGAATTTATATGTATG+AGG - chr5_2:60390250-60390269 None:intergenic 20.0%
!!! AAGTTTTCTTTTGCAAAAAT+TGG + chr5_2:60390881-60390900 Msa0779220:CDS 20.0%
!! TATTGTGTATTGAATAAAAG+CGG + chr5_2:60391287-60391306 Msa0779220:CDS 20.0%
!! AAGAAAGAAAGAAAAAAGAA+AGG + chr5_2:60391554-60391573 Msa0779220:intron 20.0%
!!! TCTTTGTTTATAATGTGAAT+CGG + chr5_2:60391832-60391851 Msa0779220:intron 20.0%
!!! TTGATGATAATATAACTCAT+CGG - chr5_2:60392034-60392053 None:intergenic 20.0%
!! AATATTGTTATAAAGTCTTC+TGG - chr5_2:60392087-60392106 None:intergenic 20.0%
!! GAAGACTTTATAACAATATT+TGG + chr5_2:60392087-60392106 Msa0779220:CDS 20.0%
!! AAATACTATTCTATAGGATA+CGG + chr5_2:60392225-60392244 Msa0779220:CDS 20.0%
!! TACTGATTGTACAATTTGTT+TGG + chr5_2:60390213-60390232 Msa0779220:intron 25.0%
! GAGAGTAATTACTTAAATTG+TGG + chr5_2:60390701-60390720 Msa0779220:intron 25.0%
! ACGAGTTTATAACAAGATTT+GGG + chr5_2:60390779-60390798 Msa0779220:CDS 25.0%
!! TATGTAGATTTCAAGTATGA+AGG + chr5_2:60390943-60390962 Msa0779220:intron 25.0%
!! ATGTAGATTTCAAGTATGAA+GGG + chr5_2:60390944-60390963 Msa0779220:intron 25.0%
!!! AAAAAGAAAGGTAGTTTTGT+TGG + chr5_2:60391566-60391585 Msa0779220:intron 25.0%
! ACTAATAGATATCATCAATG+AGG + chr5_2:60391607-60391626 Msa0779220:intron 25.0%
! CTTATTACTTATTGAATTGC+AGG + chr5_2:60391881-60391900 Msa0779220:intron 25.0%
! GGTGAAATTTACAATTTCTT+TGG - chr5_2:60391970-60391989 None:intergenic 25.0%
! TACTTGTCTTTCAAATATGA+AGG + chr5_2:60392252-60392271 Msa0779220:intron 25.0%
! ACTTGTCTTTCAAATATGAA+GGG + chr5_2:60392253-60392272 Msa0779220:intron 25.0%
!!! TTAGTACTTAGAGTTTTGAA+TGG - chr5_2:60392333-60392352 None:intergenic 25.0%
! TTCAAAACTCTAAGTACTAA+TGG + chr5_2:60392333-60392352 Msa0779220:CDS 25.0%
!! AACTAAAATGAAGGTTTTTG+GGG - chr5_2:60392491-60392510 None:intergenic 25.0%
!! GAACTAAAATGAAGGTTTTT+GGG - chr5_2:60392492-60392511 None:intergenic 25.0%
!! TGAACTAAAATGAAGGTTTT+TGG - chr5_2:60392493-60392512 None:intergenic 25.0%
!! GGATTTTACTTTGTAGTTTT+TGG + chr5_2:60392541-60392560 Msa0779220:intron 25.0%
! ACTATTTCTTTGGTATGCAT+GGG - chr5_2:60389978-60389997 None:intergenic 30.0%
! TACTATTTCTTTGGTATGCA+TGG - chr5_2:60389979-60389998 None:intergenic 30.0%
! AGTGAAGCATACTATTTCTT+TGG - chr5_2:60389988-60390007 None:intergenic 30.0%
TTTGTATGTTTGGACATTCA+AGG + chr5_2:60390481-60390500 Msa0779220:intron 30.0%
TTAGTGTGTCTTTCATTCTA+TGG + chr5_2:60390520-60390539 Msa0779220:intron 30.0%
! AATCTTGTTATAAACTCGTC+TGG - chr5_2:60390778-60390797 None:intergenic 30.0%
GACGAGTTTATAACAAGATT+TGG + chr5_2:60390778-60390797 Msa0779220:CDS 30.0%
ATTTCCTATCCATTCAAAAC+TGG + chr5_2:60391012-60391031 Msa0779220:CDS 30.0%
TTTCCTATCCATTCAAAACT+GGG + chr5_2:60391013-60391032 Msa0779220:CDS 30.0%
AAATCAATGCGAATAACGTT+TGG + chr5_2:60391314-60391333 Msa0779220:CDS 30.0%
TTGGATTCTTTAGATTTGCA+AGG + chr5_2:60391333-60391352 Msa0779220:CDS 30.0%
TGGATTCTTTAGATTTGCAA+GGG + chr5_2:60391334-60391353 Msa0779220:CDS 30.0%
TATGAGTTTGAGCTTATCAA+GGG + chr5_2:60391383-60391402 Msa0779220:CDS 30.0%
GATATCATCAATGAGGAATA+TGG + chr5_2:60391614-60391633 Msa0779220:intron 30.0%
AAGAATAATGAACTCGTATG+TGG - chr5_2:60391816-60391835 None:intergenic 30.0%
ATTACTTATTGAATTGCAGG+CGG + chr5_2:60391884-60391903 Msa0779220:intron 30.0%
!! GGAATCCAAACATTTTAAGA+AGG + chr5_2:60391920-60391939 Msa0779220:CDS 30.0%
TATGGCCTTCTTAAAATGTT+TGG - chr5_2:60391928-60391947 None:intergenic 30.0%
ACAATTTCTTTGGCATGAAT+GGG - chr5_2:60391960-60391979 None:intergenic 30.0%
TACAATTTCTTTGGCATGAA+TGG - chr5_2:60391961-60391980 None:intergenic 30.0%
AAAATGGAGTTGAAGAAATG+TGG + chr5_2:60392162-60392181 Msa0779220:CDS 30.0%
! ACGTTTTCTTTTGCAACAAA+TGG + chr5_2:60392190-60392209 Msa0779220:CDS 30.0%
TGTCTAAATGTGCTTCTAAA+AGG + chr5_2:60392439-60392458 Msa0779220:CDS 30.0%
! GTCTAAATGTGCTTCTAAAA+GGG + chr5_2:60392440-60392459 Msa0779220:CDS 30.0%
TGCACTTTGAACTAAAATGA+AGG - chr5_2:60392500-60392519 None:intergenic 30.0%
! ATTTGCATTGCTGATGTTTT+GGG - chr5_2:60392734-60392753 None:intergenic 30.0%
! AAATGTTTGGAATCCTTCTC+CGG - chr5_2:60389933-60389952 None:intergenic 35.0%
TCTAATGCATGCACGATTTA+TGG - chr5_2:60390037-60390056 None:intergenic 35.0%
!! CACAAAAAGAGATTTTCGAG+CGG - chr5_2:60390423-60390442 None:intergenic 35.0%
! GTGTGACTCTTTTGTATGTT+TGG + chr5_2:60390471-60390490 Msa0779220:intron 35.0%
!! TGAGAGTGACTTAAGTTGAA+TGG + chr5_2:60390567-60390586 Msa0779220:intron 35.0%
TTACTCTCATGAAAAGCTTC+AGG - chr5_2:60390689-60390708 None:intergenic 35.0%
CATATGGAAGTAAAGAAACG+TGG + chr5_2:60390853-60390872 Msa0779220:CDS 35.0%
AACCATAACAGATAGAGTAG+TGG - chr5_2:60390921-60390940 None:intergenic 35.0%
!!! TTAGTCTTCCCAGTTTTGAA+TGG - chr5_2:60391024-60391043 None:intergenic 35.0%
TTCAAAACTGGGAAGACTAA+TGG + chr5_2:60391024-60391043 Msa0779220:CDS 35.0%
! AACGAAAAGAAGGGCTTTTT+GGG - chr5_2:60391059-60391078 None:intergenic 35.0%
ATATCGACAGTCAAAGGATT+GGG - chr5_2:60391082-60391101 None:intergenic 35.0%
TTCATGCTCCAAAATTCTCA+AGG + chr5_2:60391248-60391267 Msa0779220:CDS 35.0%
! ACTGCTTTCCTTGAGAATTT+TGG - chr5_2:60391259-60391278 None:intergenic 35.0%
AAAATTCTCAAGGAAAGCAG+TGG + chr5_2:60391258-60391277 Msa0779220:CDS 35.0%
AAATTCTCAAGGAAAGCAGT+GGG + chr5_2:60391259-60391278 Msa0779220:CDS 35.0%
TGTGTATTGAATAAAAGCGG+TGG + chr5_2:60391290-60391309 Msa0779220:CDS 35.0%
AGGGAAAACAATCTATCAGA+TGG + chr5_2:60391353-60391372 Msa0779220:CDS 35.0%
GTATGAGTTTGAGCTTATCA+AGG + chr5_2:60391382-60391401 Msa0779220:CDS 35.0%
ATGAGTTTGAGCTTATCAAG+GGG + chr5_2:60391384-60391403 Msa0779220:CDS 35.0%
!!! AGGTAGTTTTGTTGGTTGTT+AGG + chr5_2:60391574-60391593 Msa0779220:intron 35.0%
!! TAACTCATCGGTTCTACTAT+AGG - chr5_2:60392022-60392041 None:intergenic 35.0%
! ATTTTCCAAATGAGACCATC+CGG - chr5_2:60392147-60392166 None:intergenic 35.0%
AAGTGAGAGTCAAGAGAAAA+AGG + chr5_2:60392407-60392426 Msa0779220:CDS 35.0%
!! TAAAATGAAGGTTTTTGGGG+TGG - chr5_2:60392488-60392507 None:intergenic 35.0%
!!! TTTTAGTTCAAAGTGCACCA+AGG + chr5_2:60392503-60392522 Msa0779220:CDS 35.0%
! TTTAGTTCAAAGTGCACCAA+GGG + chr5_2:60392504-60392523 Msa0779220:CDS 35.0%
GGATAAGCGATCAATTTAAG+TGG - chr5_2:60392597-60392616 None:intergenic 35.0%
AGAAGTAACATACTGCACAA+TGG - chr5_2:60392618-60392637 None:intergenic 35.0%
!!! TGAGAATTTTGGAGCTTGAT+AGG - chr5_2:60392674-60392693 None:intergenic 35.0%
ATCAAGCTCCAAAATTCTCA+TGG + chr5_2:60392674-60392693 Msa0779220:CDS 35.0%
! TTTGCATTGCTGATGTTTTG+GGG - chr5_2:60392733-60392752 None:intergenic 35.0%
! CATTTGCATTGCTGATGTTT+TGG - chr5_2:60392735-60392754 None:intergenic 35.0%
CAAAACATCAGCAATGCAAA+TGG + chr5_2:60392733-60392752 Msa0779220:CDS 35.0%
AGGGATTTATGAAATTCGCT+AGG + chr5_2:60392759-60392778 Msa0779220:CDS 35.0%
GGGATTTATGAAATTCGCTA+GGG + chr5_2:60392760-60392779 Msa0779220:CDS 35.0%
! ATTGTGACTTTTAGCACAAC+CGG - chr5_2:60392839-60392858 None:intergenic 35.0%
TTTCTTTGGTATGCATGGGA+GGG - chr5_2:60389974-60389993 None:intergenic 40.0%
ATTTCTTTGGTATGCATGGG+AGG - chr5_2:60389975-60389994 None:intergenic 40.0%
GGTAACTGAAAACGAAAGTG+AGG - chr5_2:60390016-60390035 None:intergenic 40.0%
! ATCACCCTTCCTCTAAAGAT+TGG + chr5_2:60390737-60390756 Msa0779220:intron 40.0%
! GTGACCAATCTTTAGAGGAA+GGG - chr5_2:60390744-60390763 None:intergenic 40.0%
! AGTGACCAATCTTTAGAGGA+AGG - chr5_2:60390745-60390764 None:intergenic 40.0%
CTGAAGTGACCAATCTTTAG+AGG - chr5_2:60390749-60390768 None:intergenic 40.0%
ATATGCCATTCACAACCATC+AGG - chr5_2:60390838-60390857 None:intergenic 40.0%
TGATGGTTGTGAATGGCATA+TGG + chr5_2:60390837-60390856 Msa0779220:CDS 40.0%
GACCACTACTCTATCTGTTA+TGG + chr5_2:60390916-60390935 Msa0779220:CDS 40.0%
!!! CTTCCCAGTTTTGAATGGAT+AGG - chr5_2:60391019-60391038 None:intergenic 40.0%
!!! AAAGAAGGGCTTTTTGGGTT+TGG - chr5_2:60391054-60391073 None:intergenic 40.0%
! GAACGAAAAGAAGGGCTTTT+TGG - chr5_2:60391060-60391079 None:intergenic 40.0%
AGGATTGGGAACGAAAAGAA+GGG - chr5_2:60391068-60391087 None:intergenic 40.0%
AAGGATTGGGAACGAAAAGA+AGG - chr5_2:60391069-60391088 None:intergenic 40.0%
CATATCGACAGTCAAAGGAT+TGG - chr5_2:60391083-60391102 None:intergenic 40.0%
ATCCTTTGACTGTCGATATG+CGG + chr5_2:60391083-60391102 Msa0779220:CDS 40.0%
AGGAACATACTACACAATGG+AGG - chr5_2:60391189-60391208 None:intergenic 40.0%
AGCAGGAACATACTACACAA+TGG - chr5_2:60391192-60391211 None:intergenic 40.0%
TGGAGCATGAACAGAACATT+TGG - chr5_2:60391239-60391258 None:intergenic 40.0%
! GTAACTTGTAACACAACACG+CGG - chr5_2:60391413-60391432 None:intergenic 40.0%
ACAAGTTACAGCTTCTCACA+CGG + chr5_2:60391424-60391443 Msa0779220:CDS 40.0%
TGTTGCTACAGTTACTGTTC+CGG + chr5_2:60392125-60392144 Msa0779220:CDS 40.0%
GGATGGTCTCATTTGGAAAA+TGG + chr5_2:60392146-60392165 Msa0779220:CDS 40.0%
GGACTATGACACTCTTTGTT+TGG - chr5_2:60392363-60392382 None:intergenic 40.0%
!! ACAAAGAGTGTCATAGTCCT+AGG + chr5_2:60392364-60392383 Msa0779220:CDS 40.0%
AGTGTCATAGTCCTAGGAAA+AGG + chr5_2:60392370-60392389 Msa0779220:CDS 40.0%
TGGAGCTTGATAGGACTATT+CGG - chr5_2:60392665-60392684 None:intergenic 40.0%
! ACTGCTCTCCATGAGAATTT+TGG - chr5_2:60392685-60392704 None:intergenic 40.0%
AAAATTCTCATGGAGAGCAG+TGG + chr5_2:60392684-60392703 Msa0779220:CDS 40.0%
AAATTCTCATGGAGAGCAGT+GGG + chr5_2:60392685-60392704 Msa0779220:CDS 40.0%
ATCAGCAATGCAAATGGTGA+AGG + chr5_2:60392739-60392758 Msa0779220:CDS 40.0%
TCAGCAATGCAAATGGTGAA+GGG + chr5_2:60392740-60392759 Msa0779220:CDS 40.0%
TGAGCTAATCAAGAAGAAGC+CGG + chr5_2:60392817-60392836 Msa0779220:CDS 40.0%
AATCACTATACTCAACCACG+CGG - chr5_2:60392865-60392884 None:intergenic 40.0%
! GTGACGGAACAAATCTTCTC+CGG - chr5_2:60389905-60389924 None:intergenic 45.0%
! GAGAAGATTTGTTCCGTCAC+CGG + chr5_2:60389905-60389924 Msa0779220:CDS 45.0%
TTCTTTGGTATGCATGGGAG+GGG - chr5_2:60389973-60389992 None:intergenic 45.0%
GCTACGATTACTGTTCCTGA+TGG + chr5_2:60390820-60390839 Msa0779220:CDS 45.0%
CTGTTCCTGATGGTTGTGAA+TGG + chr5_2:60390830-60390849 Msa0779220:CDS 45.0%
ATAGAGTAGTGGTCTGCGAA+CGG - chr5_2:60390910-60390929 None:intergenic 45.0%
TACTCTGCAGCAAATTCAGC+AGG - chr5_2:60391209-60391228 None:intergenic 45.0%
CGTCATACGAGTTCATGCAT+TGG - chr5_2:60391691-60391710 None:intergenic 45.0%
TATTGAATTGCAGGCGGAGA+AGG + chr5_2:60391890-60391909 Msa0779220:intron 45.0%
!! ATGAATGGGTGACGGAAGTA+TGG - chr5_2:60391946-60391965 None:intergenic 45.0%
TCTTTGGCATGAATGGGTGA+CGG - chr5_2:60391954-60391973 None:intergenic 45.0%
GGAGTTGAAGAAATGTGGCA+AGG + chr5_2:60392167-60392186 Msa0779220:CDS 45.0%
TAGTCCTAGGAAAAGGTCCA+AGG + chr5_2:60392377-60392396 Msa0779220:CDS 45.0%
ACTCTCACTTGTTTCGACCT+TGG - chr5_2:60392397-60392416 None:intergenic 45.0%
! ATGTGCTTCTAAAAGGGCTG+AGG + chr5_2:60392446-60392465 Msa0779220:CDS 45.0%
!!! GTTTTTGGGGTGGAATGCAT+TGG - chr5_2:60392478-60392497 None:intergenic 45.0%
AAAAGTCACAATGTTCCGCG+TGG + chr5_2:60392847-60392866 Msa0779220:CDS 45.0%
TCTTTGGTATGCATGGGAGG+GGG - chr5_2:60389972-60389991 None:intergenic 50.0%
GCTTCAGGTAAGTGCGTGTT+TGG - chr5_2:60390674-60390693 None:intergenic 50.0%
CACCGCATATCGACAGTCAA+AGG - chr5_2:60391088-60391107 None:intergenic 50.0%
AATGAGAGAGAGAGAGCGAG+AGG - chr5_2:60391991-60392010 None:intergenic 50.0%
GCTACAGTTACTGTTCCGGA+TGG + chr5_2:60392129-60392148 Msa0779220:CDS 50.0%
CTGTTCCGGATGGTCTCATT+TGG + chr5_2:60392139-60392158 Msa0779220:CDS 50.0%
! TCGACCTTGGACCTTTTCCT+AGG - chr5_2:60392384-60392403 None:intergenic 50.0%
GTGCACCAAGGGAAGATATG+CGG + chr5_2:60392515-60392534 Msa0779220:CDS 50.0%
GGAACAAATCTTCTCCGGCG+AGG - chr5_2:60389900-60389919 None:intergenic 55.0%
ATGCATGGGAGGGGGAAGAA+TGG - chr5_2:60389964-60389983 None:intergenic 55.0%
TATGTGGCGTGACGTGTGAG+CGG - chr5_2:60391800-60391819 None:intergenic 55.0%
TGCAGGCGGAGAAGGAAGTT+AGG + chr5_2:60391898-60391917 Msa0779220:intron 55.0%
GCAGGCGGAGAAGGAAGTTA+GGG + chr5_2:60391899-60391918 Msa0779220:intron 55.0%
CCTCACCGCATATCTTCCCT+TGG - chr5_2:60392523-60392542 None:intergenic 55.0%
CCAAGGGAAGATATGCGGTG+AGG + chr5_2:60392520-60392539 Msa0779220:intron 55.0%
ATTTGTTCCGTCACCGGCGC+CGG + chr5_2:60389911-60389930 Msa0779220:CDS 60.0%
TTGGAATCCTTCTCCGGCGC+CGG - chr5_2:60389927-60389946 None:intergenic 60.0%
!! TCCTTCTCCGGCGCCGGTGA+CGG - chr5_2:60389921-60389940 None:intergenic 70.0%
! TCCGTCACCGGCGCCGGAGA+AGG + chr5_2:60389917-60389936 Msa0779220:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 60389891 60392886 60389891 ID=Msa0779220;Name=Msa0779220
chr5_2 mRNA 60389891 60392886 60389891 ID=Msa0779220-mRNA-1;Parent=Msa0779220;Name=Msa0779220-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.28|0|1|0.71|0.62|8|0|478
chr5_2 exon 60389891 60389995 60389891 ID=Msa0779220-mRNA-1:exon:17694;Parent=Msa0779220-mRNA-1
chr5_2 exon 60390769 60390937 60390769 ID=Msa0779220-mRNA-1:exon:17695;Parent=Msa0779220-mRNA-1
chr5_2 exon 60390950 60391104 60390950 ID=Msa0779220-mRNA-1:exon:17696;Parent=Msa0779220-mRNA-1
chr5_2 exon 60391200 60391445 60391200 ID=Msa0779220-mRNA-1:exon:17697;Parent=Msa0779220-mRNA-1
chr5_2 exon 60391903 60391977 60391903 ID=Msa0779220-mRNA-1:exon:17698;Parent=Msa0779220-mRNA-1
chr5_2 exon 60392078 60392256 60392078 ID=Msa0779220-mRNA-1:exon:17699;Parent=Msa0779220-mRNA-1
chr5_2 exon 60392290 60392536 60392290 ID=Msa0779220-mRNA-1:exon:17700;Parent=Msa0779220-mRNA-1
chr5_2 exon 60392626 60392886 60392626 ID=Msa0779220-mRNA-1:exon:17701;Parent=Msa0779220-mRNA-1
chr5_2 CDS 60389891 60389995 60389891 ID=Msa0779220-mRNA-1:cds;Parent=Msa0779220-mRNA-1
chr5_2 CDS 60390769 60390937 60390769 ID=Msa0779220-mRNA-1:cds;Parent=Msa0779220-mRNA-1
chr5_2 CDS 60390950 60391104 60390950 ID=Msa0779220-mRNA-1:cds;Parent=Msa0779220-mRNA-1
chr5_2 CDS 60391200 60391445 60391200 ID=Msa0779220-mRNA-1:cds;Parent=Msa0779220-mRNA-1
chr5_2 CDS 60391903 60391977 60391903 ID=Msa0779220-mRNA-1:cds;Parent=Msa0779220-mRNA-1
chr5_2 CDS 60392078 60392256 60392078 ID=Msa0779220-mRNA-1:cds;Parent=Msa0779220-mRNA-1
chr5_2 CDS 60392290 60392536 60392290 ID=Msa0779220-mRNA-1:cds;Parent=Msa0779220-mRNA-1
chr5_2 CDS 60392626 60392886 60392626 ID=Msa0779220-mRNA-1:cds;Parent=Msa0779220-mRNA-1
Gene Sequence

>Msa0779220

ATGCAGCCTCGCCGGAGAAGATTTGTTCCGTCACCGGCGCCGGAGAAGGATTCCAAACATTTTATGAAAACCATTCTTCCCCCTCCCATGCATACCAAAGAAATAAGAATTCCAGACGAGTTTATAACAAGATTTGGGAATGAACTCAACAGTGTTGCTACGATTACTGTTCCTGATGGTTGTGAATGGCATATGGAAGTAAAGAAACGTGGCAACGAAGTTTTCTTTTGCAAAAATTGGCAACCGTTCGCAGACCACTACTCTATCTGTTATGATTTCAAGTATGAAGGGAATTCAAAGTTTAATGTTGTTATATATGATACAAATTCTGTCGAGATTTCCTATCCATTCAAAACTGGGAAGACTAATGGAGAACCAAACCCAAAAAGCCCTTCTTTTCGTTCCCAATCCTTTGACTGTCGATATGCGTATGTTCCTGCTGAATTTGCTGCAGAGTATTTGAAACCAAATGTTCTGTTCATGCTCCAAAATTCTCAAGGAAAGCAGTGGGAAGTTTATTGTGTATTGAATAAAAGCGGTGGAAAATCAATGCGAATAACGTTTGGATTCTTTAGATTTGCAAGGGAAAACAATCTATCAGATGGAGTTATGTATGAGTTTGAGCTTATCAAGGGGAAGCCGCGTGTTGTGTTACAAGTTACAGCTTCTCACACGGCGGAGAAGGAAGTTAGGGAATCCAAACATTTTAAGAAGGCCATACTTCCGTCACCCATTCATGCCAAAGAAATTAGAATTCCAGAAGACTTTATAACAATATTTGGGAATGAACTCAAGAATGTTGCTACAGTTACTGTTCCGGATGGTCTCATTTGGAAAATGGAGTTGAAGAAATGTGGCAAGGACGTTTTCTTTTGCAACAAATGGCAACCGTTTTCAAAATACTATTCTATAGGATACGGTTGTTACTTTGTTATTATATTTGATGCAACTTCTGTTGAAATTTGCTATCCATTCAAAACTCTAAGTACTAATGGAGAACCAAACAAAGAGTGTCATAGTCCTAGGAAAAGGTCCAAGGTCGAAACAAGTGAGAGTCAAGAGAAAAAGGTCAAAAACATGTCTAAATGTGCTTCTAAAAGGGCTGAGGATGTTGCCAATGCATTCCACCCCAAAAACCTTCATTTTAGTTCAAAGTGCACCAAGGGAAGATATGCGTATGTTACTTCTAAATTTGCTTCAAAGTATCTGAAGCCGAATAGTCCTATCAAGCTCCAAAATTCTCATGGAGAGCAGTGGGAAGTTTCTAGTATATTCTATAACCCCAAAACATCAGCAATGCAAATGGTGAAGGGATTTATGAAATTCGCTAGGGACAACAATCTGTCACATGAAGATTATTGTGTGTTTGAGCTAATCAAGAAGAAGCCGGTTGTGCTAAAAGTCACAATGTTCCGCGTGGTTGAGTATAGTGATTAA
Protein sequence

>Msa0779220

MQPRRRRFVPSPAPEKDSKHFMKTILPPPMHTKEIRIPDEFITRFGNELNSVATITVPDGCEWHMEVKKRGNEVFFCKNWQPFADHYSICYDFKYEGNSKFNVVIYDTNSVEISYPFKTGKTNGEPNPKSPSFRSQSFDCRYAYVPAEFAAEYLKPNVLFMLQNSQGKQWEVYCVLNKSGGKSMRITFGFFRFARENNLSDGVMYEFELIKGKPRVVLQVTASHTAEKEVRESKHFKKAILPSPIHAKEIRIPEDFITIFGNELKNVATVTVPDGLIWKMELKKCGKDVFFCNKWQPFSKYYSIGYGCYFVIIFDATSVEICYPFKTLSTNGEPNKECHSPRKRSKVETSESQEKKVKNMSKCASKRAEDVANAFHPKNLHFSSKCTKGRYAYVTSKFASKYLKPNSPIKLQNSHGEQWEVSSIFYNPKTSAMQMVKGFMKFARDNNLSHEDYCVFELIKKKPVVLKVTMFRVVEYSD*