Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0780420 | sp|Q39202|RLK1_ARATH | 34.914 | 232 | 117 | 7 | 2 | 200 | 380 | 610 | 9.64e-28 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0780420 | G7JXF4 | 71.689 | 219 | 43 | 2 | 1 | 200 | 372 | 590 | 1.65e-98 | 313 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0780420 | MtrunA17_Chr5g0399561 | 71.689 | 219 | 43 | 2 | 1 | 200 | 372 | 590 | 3.17e-102 | 313 |
| Msa0780420 | MtrunA17_Chr5g0399591 | 64.516 | 217 | 60 | 2 | 1 | 200 | 371 | 587 | 6.74e-91 | 283 |
| Msa0780420 | MtrunA17_Chr2g0311521 | 37.118 | 229 | 124 | 5 | 2 | 210 | 601 | 829 | 1.28e-37 | 139 |
| Msa0780420 | MtrunA17_Chr2g0311541 | 36.239 | 218 | 120 | 5 | 2 | 200 | 359 | 576 | 3.00e-34 | 129 |
| Msa0780420 | MtrunA17_Chr2g0311551 | 34.513 | 226 | 131 | 3 | 2 | 210 | 598 | 823 | 1.52e-33 | 127 |
| Msa0780420 | MtrunA17_Chr8g0338471 | 32.927 | 246 | 127 | 9 | 1 | 210 | 365 | 608 | 8.56e-32 | 122 |
| Msa0780420 | MtrunA17_Chr8g0338501 | 29.268 | 246 | 138 | 10 | 1 | 210 | 394 | 639 | 3.47e-27 | 108 |
| Msa0780420 | MtrunA17_Chr8g0338531 | 32.639 | 144 | 70 | 5 | 84 | 200 | 388 | 531 | 7.23e-14 | 70.1 |
| Msa0780420 | MtrunA17_Chr2g0311511 | 37.143 | 105 | 50 | 3 | 112 | 200 | 418 | 522 | 1.93e-12 | 65.9 |
| Msa0780420 | MtrunA17_Chr5g0394381 | 30.151 | 199 | 109 | 6 | 39 | 210 | 384 | 579 | 3.56e-12 | 65.1 |
| Msa0780420 | MtrunA17_Chr4g0063701 | 26.724 | 232 | 125 | 8 | 8 | 200 | 379 | 604 | 3.74e-12 | 65.1 |
| Msa0780420 | MtrunA17_Chr4g0063691 | 25.217 | 230 | 131 | 7 | 20 | 210 | 398 | 625 | 4.55e-11 | 62.0 |
| Msa0780420 | MtrunA17_Chr3g0144551 | 38.202 | 89 | 40 | 1 | 127 | 200 | 121 | 209 | 6.76e-11 | 61.2 |
| Msa0780420 | MtrunA17_Chr5g0402071 | 38.043 | 92 | 36 | 2 | 127 | 200 | 154 | 242 | 8.46e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0780420 | AT5G24080.2 | 28.505 | 214 | 129 | 5 | 21 | 210 | 402 | 615 | 8.53e-12 | 64.7 |
| Msa0780420 | AT5G24080.3 | 28.505 | 214 | 129 | 5 | 21 | 210 | 275 | 488 | 8.72e-12 | 64.7 |
| Msa0780420 | AT5G24080.1 | 28.505 | 214 | 129 | 5 | 21 | 210 | 275 | 488 | 8.72e-12 | 64.7 |
Find 42 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCTTGGCGGCAGTTGTTT+TGG | 0.307589 | 5_2:-62133369 | Msa0780420:CDS |
| TGAGACTCCTGTTGAGAATA+AGG | 0.326123 | 5_2:+62133635 | None:intergenic |
| ACCAACATAAATTTGTTTCC+AGG | 0.334800 | 5_2:+62133303 | None:intergenic |
| TTAATCTTACAAATATGAAT+TGG | 0.372926 | 5_2:-62133685 | None:intergenic |
| ATTCTTCAAGGTTCACTCAT+TGG | 0.373322 | 5_2:-62133413 | Msa0780420:CDS |
| ATGAGTGAACCTTGAAGAAT+AGG | 0.376884 | 5_2:+62133416 | None:intergenic |
| CAAGTTAACCCTATTCTTCA+AGG | 0.377870 | 5_2:-62133425 | Msa0780420:CDS |
| AAAAGATCGCCTCTTGCTAA+TGG | 0.396495 | 5_2:-62133548 | Msa0780420:CDS |
| ATCCGTTCATAATCTGATAA+AGG | 0.396851 | 5_2:+62133662 | None:intergenic |
| TGTCACAGGCGCAACTCTCC+TGG | 0.398061 | 5_2:-62133321 | Msa0780420:CDS |
| TATTGTAGCAATTAAGAGAT+TGG | 0.411792 | 5_2:-62133170 | Msa0780420:CDS |
| CTTGAAGAATAGGGTTAACT+TGG | 0.414114 | 5_2:+62133426 | None:intergenic |
| AATACAAGACTTTCTCCTCT+TGG | 0.458232 | 5_2:-62133491 | Msa0780420:intron |
| GATGCAGCCTTATTCTCAAC+AGG | 0.469801 | 5_2:-62133642 | Msa0780420:CDS |
| TCCTGGAAACAAATTTATGT+TGG | 0.476021 | 5_2:-62133304 | Msa0780420:CDS |
| TGACTTTGTTGAAACCGAAA+AGG | 0.476315 | 5_2:-62133346 | Msa0780420:CDS |
| TGAGTGAACCTTGAAGAATA+GGG | 0.482650 | 5_2:+62133417 | None:intergenic |
| AAGACTGAGCTGAGGGCCAT+TGG | 0.491680 | 5_2:-62133115 | Msa0780420:CDS |
| TCTTTCTTCGAATCCGTGGA+AGG | 0.501408 | 5_2:+62133452 | None:intergenic |
| CAGTCTTAAATTCTTTCTCC+CGG | 0.501544 | 5_2:+62133131 | None:intergenic |
| CTTGCTAATGGAAGAGAAGA+TGG | 0.512984 | 5_2:-62133536 | Msa0780420:CDS |
| GTCGTCTTTCTTCGAATCCG+TGG | 0.516455 | 5_2:+62133448 | None:intergenic |
| TCATCTCTGAAGACAACAGC+AGG | 0.518288 | 5_2:+62133584 | None:intergenic |
| GGCCTTTATCAGATTATGAA+CGG | 0.521585 | 5_2:-62133664 | Msa0780420:CDS |
| CTTCAATAGCATACTCTTGG+CGG | 0.530974 | 5_2:-62133381 | Msa0780420:CDS |
| TGTCTTCAATAGCATACTCT+TGG | 0.531087 | 5_2:-62133384 | Msa0780420:CDS |
| TTGATTGGATATAGTGATGA+GGG | 0.532159 | 5_2:-62133064 | Msa0780420:CDS |
| AGTAGTGAAGAAGTTTCTGA+AGG | 0.540866 | 5_2:+62132982 | None:intergenic |
| GACTTTGTTGAAACCGAAAA+GGG | 0.542523 | 5_2:-62133345 | Msa0780420:CDS |
| GTAGTGAAGAAGTTTCTGAA+GGG | 0.573755 | 5_2:+62132983 | None:intergenic |
| AAACCGAAAAGGGTTGTCAC+AGG | 0.573900 | 5_2:-62133335 | Msa0780420:CDS |
| TCTTCTCTTCCATTAGCAAG+AGG | 0.583375 | 5_2:+62133539 | None:intergenic |
| AACAGGTACTAGTCCTTCCA+CGG | 0.593175 | 5_2:-62133465 | Msa0780420:intron |
| GGATCGGTTAGCGCACGACC+GGG | 0.604005 | 5_2:-62133149 | Msa0780420:CDS |
| GTTGATTGGATATAGTGATG+AGG | 0.615840 | 5_2:-62133065 | Msa0780420:CDS |
| AGAATTTAAGACTGAGCTGA+GGG | 0.630447 | 5_2:-62133122 | Msa0780420:CDS |
| TAGCAATTAAGAGATTGGAT+CGG | 0.631876 | 5_2:-62133165 | Msa0780420:CDS |
| AAGAATTTAAGACTGAGCTG+AGG | 0.632459 | 5_2:-62133123 | Msa0780420:CDS |
| TTCTGAAGGGAATAAGTGTG+TGG | 0.662482 | 5_2:+62132996 | None:intergenic |
| AATGGAAGAGAAGATGGTGG+TGG | 0.686416 | 5_2:-62133530 | Msa0780420:CDS |
| GAGTTAAAAGAAACTACACA+TGG | 0.724835 | 5_2:-62133269 | Msa0780420:intron |
| GCTAATGGAAGAGAAGATGG+TGG | 0.775010 | 5_2:-62133533 | Msa0780420:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGGTTTTTATTGAAGAACT+AGG | - | chr5_2:62133376-62133395 | Msa0780420:CDS | 25.0% |
| !!! | TCCTTTGGAATTGTTTAAAA+AGG | - | chr5_2:62133406-62133425 | Msa0780420:CDS | 25.0% |
| !! | ACCTTTTTAAACAATTCCAA+AGG | + | chr5_2:62133410-62133429 | None:intergenic | 25.0% |
| ! | TATTGTAGCAATTAAGAGAT+TGG | - | chr5_2:62133456-62133475 | Msa0780420:CDS | 25.0% |
| ATCCGTTCATAATCTGATAA+AGG | + | chr5_2:62132967-62132986 | None:intergenic | 30.0% | |
| TCTTCAGAGATGAAAAATGT+TGG | - | chr5_2:62133052-62133071 | Msa0780420:CDS | 30.0% | |
| TCCTGGAAACAAATTTATGT+TGG | - | chr5_2:62133322-62133341 | Msa0780420:CDS | 30.0% | |
| ! | ACCAACATAAATTTGTTTCC+AGG | + | chr5_2:62133326-62133345 | None:intergenic | 30.0% |
| GAGTTAAAAGAAACTACACA+TGG | - | chr5_2:62133357-62133376 | Msa0780420:CDS | 30.0% | |
| TTTATTGAAGAACTAGGTAG+AGG | - | chr5_2:62133382-62133401 | Msa0780420:CDS | 30.0% | |
| TAGCAATTAAGAGATTGGAT+CGG | - | chr5_2:62133461-62133480 | Msa0780420:CDS | 30.0% | |
| ! | AAAAACTTGGTAAGGTTGAT+TGG | - | chr5_2:62133547-62133566 | Msa0780420:CDS | 30.0% |
| TTGATTGGATATAGTGATGA+GGG | - | chr5_2:62133562-62133581 | Msa0780420:CDS | 30.0% | |
| GGCCTTTATCAGATTATGAA+CGG | - | chr5_2:62132962-62132981 | Msa0780420:CDS | 35.0% | |
| AATACAAGACTTTCTCCTCT+TGG | - | chr5_2:62133135-62133154 | Msa0780420:CDS | 35.0% | |
| CTTGAAGAATAGGGTTAACT+TGG | + | chr5_2:62133203-62133222 | None:intergenic | 35.0% | |
| CAAGTTAACCCTATTCTTCA+AGG | - | chr5_2:62133201-62133220 | Msa0780420:CDS | 35.0% | |
| TGAGTGAACCTTGAAGAATA+GGG | + | chr5_2:62133212-62133231 | None:intergenic | 35.0% | |
| ATGAGTGAACCTTGAAGAAT+AGG | + | chr5_2:62133213-62133232 | None:intergenic | 35.0% | |
| ATTCTTCAAGGTTCACTCAT+TGG | - | chr5_2:62133213-62133232 | Msa0780420:intron | 35.0% | |
| TGTCTTCAATAGCATACTCT+TGG | - | chr5_2:62133242-62133261 | Msa0780420:intron | 35.0% | |
| TGACTTTGTTGAAACCGAAA+AGG | - | chr5_2:62133280-62133299 | Msa0780420:CDS | 35.0% | |
| GACTTTGTTGAAACCGAAAA+GGG | - | chr5_2:62133281-62133300 | Msa0780420:CDS | 35.0% | |
| CAGTCTTAAATTCTTTCTCC+CGG | + | chr5_2:62133498-62133517 | None:intergenic | 35.0% | |
| ! | AAGAATTTAAGACTGAGCTG+AGG | - | chr5_2:62133503-62133522 | Msa0780420:CDS | 35.0% |
| ! | AGAATTTAAGACTGAGCTGA+GGG | - | chr5_2:62133504-62133523 | Msa0780420:CDS | 35.0% |
| TAAAACCTGCCACAAAAACT+TGG | - | chr5_2:62133534-62133553 | Msa0780420:CDS | 35.0% | |
| ! | TCAACCTTACCAAGTTTTTG+TGG | + | chr5_2:62133546-62133565 | None:intergenic | 35.0% |
| GTTGATTGGATATAGTGATG+AGG | - | chr5_2:62133561-62133580 | Msa0780420:CDS | 35.0% | |
| GTAGTGAAGAAGTTTCTGAA+GGG | + | chr5_2:62133646-62133665 | None:intergenic | 35.0% | |
| AGTAGTGAAGAAGTTTCTGA+AGG | + | chr5_2:62133647-62133666 | None:intergenic | 35.0% | |
| TGAGACTCCTGTTGAGAATA+AGG | + | chr5_2:62132994-62133013 | None:intergenic | 40.0% | |
| AAAAGATCGCCTCTTGCTAA+TGG | - | chr5_2:62133078-62133097 | Msa0780420:CDS | 40.0% | |
| TCTTCTCTTCCATTAGCAAG+AGG | + | chr5_2:62133090-62133109 | None:intergenic | 40.0% | |
| CTTGCTAATGGAAGAGAAGA+TGG | - | chr5_2:62133090-62133109 | Msa0780420:CDS | 40.0% | |
| CTTTCTCCTCTTGGTAAAAC+AGG | - | chr5_2:62133144-62133163 | Msa0780420:CDS | 40.0% | |
| ! | CTAGTACCTGTTTTACCAAG+AGG | + | chr5_2:62133153-62133172 | None:intergenic | 40.0% |
| CTTCAATAGCATACTCTTGG+CGG | - | chr5_2:62133245-62133264 | Msa0780420:intron | 40.0% | |
| ! | TTTGTGGCAGGTTTTACCAA+TGG | + | chr5_2:62133530-62133549 | None:intergenic | 40.0% |
| ! | TTCTGAAGGGAATAAGTGTG+TGG | + | chr5_2:62133633-62133652 | None:intergenic | 40.0% |
| !! | GATGCAGCCTTATTCTCAAC+AGG | - | chr5_2:62132984-62133003 | Msa0780420:CDS | 45.0% |
| ! | TCATCTCTGAAGACAACAGC+AGG | + | chr5_2:62133045-62133064 | None:intergenic | 45.0% |
| GCTAATGGAAGAGAAGATGG+TGG | - | chr5_2:62133093-62133112 | Msa0780420:CDS | 45.0% | |
| ! | AATGGAAGAGAAGATGGTGG+TGG | - | chr5_2:62133096-62133115 | Msa0780420:CDS | 45.0% |
| AACAGGTACTAGTCCTTCCA+CGG | - | chr5_2:62133161-62133180 | Msa0780420:CDS | 45.0% | |
| TCTTTCTTCGAATCCGTGGA+AGG | + | chr5_2:62133177-62133196 | None:intergenic | 45.0% | |
| AAACCGAAAAGGGTTGTCAC+AGG | - | chr5_2:62133291-62133310 | Msa0780420:CDS | 45.0% | |
| GAACTAGGTAGAGGTTCCTT+TGG | - | chr5_2:62133391-62133410 | Msa0780420:CDS | 45.0% | |
| ! | CCTTACCAAGTTTTTGTGGC+AGG | + | chr5_2:62133542-62133561 | None:intergenic | 45.0% |
| CCTGCCACAAAAACTTGGTA+AGG | - | chr5_2:62133539-62133558 | Msa0780420:CDS | 45.0% | |
| GTCGTCTTTCTTCGAATCCG+TGG | + | chr5_2:62133181-62133200 | None:intergenic | 50.0% | |
| ! | ACTCTTGGCGGCAGTTGTTT+TGG | - | chr5_2:62133257-62133276 | Msa0780420:intron | 50.0% |
| ! | GCGCCTGTGACAACCCTTTT+CGG | + | chr5_2:62133297-62133316 | None:intergenic | 55.0% |
| AAGACTGAGCTGAGGGCCAT+TGG | - | chr5_2:62133511-62133530 | Msa0780420:CDS | 55.0% | |
| TGTCACAGGCGCAACTCTCC+TGG | - | chr5_2:62133305-62133324 | Msa0780420:CDS | 60.0% | |
| TGGATCGGTTAGCGCACGAC+CGG | - | chr5_2:62133476-62133495 | Msa0780420:intron | 60.0% | |
| GGATCGGTTAGCGCACGACC+GGG | - | chr5_2:62133477-62133496 | Msa0780420:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 62132955 | 62133693 | 62132955 | ID=Msa0780420;Name=Msa0780420 |
| chr5_2 | mRNA | 62132955 | 62133693 | 62132955 | ID=Msa0780420-mRNA-1;Parent=Msa0780420;Name=Msa0780420-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|0|0.33|3|0|226 |
| chr5_2 | exon | 62133492 | 62133693 | 62133492 | ID=Msa0780420-mRNA-1:exon:18411;Parent=Msa0780420-mRNA-1 |
| chr5_2 | exon | 62133270 | 62133482 | 62133270 | ID=Msa0780420-mRNA-1:exon:18410;Parent=Msa0780420-mRNA-1 |
| chr5_2 | exon | 62132955 | 62133220 | 62132955 | ID=Msa0780420-mRNA-1:exon:18409;Parent=Msa0780420-mRNA-1 |
| chr5_2 | CDS | 62133492 | 62133693 | 62133492 | ID=Msa0780420-mRNA-1:cds;Parent=Msa0780420-mRNA-1 |
| chr5_2 | CDS | 62133270 | 62133482 | 62133270 | ID=Msa0780420-mRNA-1:cds;Parent=Msa0780420-mRNA-1 |
| chr5_2 | CDS | 62132955 | 62133220 | 62132955 | ID=Msa0780420-mRNA-1:cds;Parent=Msa0780420-mRNA-1 |
| Gene Sequence |
| Protein sequence |
