AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0780720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0780720 MtrunA17_Chr5g0441691 92.453 159 5 1 8 159 271 429 4.37e-99 296
Msa0780720 MtrunA17_Chr0c02g0489761 84.906 159 17 1 8 159 281 439 1.09e-93 282
Msa0780720 MtrunA17_Chr8g0387671 67.901 162 43 2 7 159 266 427 1.72e-68 217
Msa0780720 MtrunA17_Chr5g0397101 67.901 162 42 3 7 159 267 427 4.98e-67 213
Msa0780720 MtrunA17_Chr4g0074811 67.296 159 45 1 8 159 294 452 2.68e-66 212
Msa0780720 MtrunA17_Chr5g0444171 63.522 159 51 1 8 159 295 453 4.51e-63 203
Msa0780720 MtrunA17_Chr3g0098951 66.026 156 45 2 8 156 287 441 1.19e-62 202
Msa0780720 MtrunA17_Chr1g0157131 64.780 159 49 1 8 159 277 435 1.49e-62 201
Msa0780720 MtrunA17_Chr1g0170281 64.151 159 50 1 8 159 321 479 2.65e-61 199
Msa0780720 MtrunA17_Chr7g0267751 63.522 159 51 1 8 159 338 496 8.32e-61 199
Msa0780720 MtrunA17_Chr1g0176751 63.522 159 51 1 8 159 327 485 1.72e-60 197
Msa0780720 MtrunA17_Chr1g0200601 62.893 159 52 1 8 159 233 391 2.81e-60 195
Msa0780720 MtrunA17_Chr1g0176421 60.377 159 56 1 8 159 364 522 1.70e-58 193
Msa0780720 MtrunA17_Chr5g0448721 59.119 159 58 1 8 159 299 457 3.02e-58 191
Msa0780720 MtrunA17_Chr8g0385341 59.748 159 57 1 8 159 233 391 1.41e-57 188
Msa0780720 MtrunA17_Chr3g0131181 58.491 159 59 1 8 159 232 390 2.20e-57 188
Msa0780720 MtrunA17_Chr5g0441771 78.846 104 2 1 29 132 18 101 2.05e-52 163
Msa0780720 MtrunA17_Chr4g0074791 56.291 151 62 2 9 159 300 446 2.48e-50 169
Msa0780720 MtrunA17_Chr4g0059951 53.459 159 67 1 8 159 276 434 6.58e-48 164
Msa0780720 MtrunA17_Chr7g0242561 50.943 159 71 1 8 159 259 417 2.48e-47 162
Msa0780720 MtrunA17_Chr7g0257751 49.057 159 74 1 8 159 261 419 2.11e-42 149
Msa0780720 MtrunA17_Chr1g0173541 42.697 178 92 2 8 175 255 432 2.91e-42 148
Msa0780720 MtrunA17_Chr5g0405421 41.875 160 85 2 8 159 304 463 6.05e-39 139
Msa0780720 MtrunA17_Chr4g0033371 43.396 159 83 1 8 159 275 433 1.11e-37 136
Msa0780720 MtrunA17_Chr7g0267771 66.667 81 27 0 8 88 291 371 1.02e-31 119
Msa0780720 MtrunA17_Chr4g0045261 33.333 132 87 1 9 140 345 475 1.69e-24 99.8
Msa0780720 MtrunA17_Chr7g0276111 31.818 132 88 1 9 140 352 481 3.31e-24 99.0
Msa0780720 MtrunA17_Chr5g0402551 32.394 142 95 1 9 150 342 482 4.64e-24 98.6
Msa0780720 MtrunA17_Chr8g0374701 34.848 132 85 1 9 140 336 466 2.37e-23 96.3
Msa0780720 MtrunA17_Chr3g0127571 34.091 132 84 2 9 140 346 474 1.03e-22 94.7
Msa0780720 MtrunA17_Chr5g0444191 56.757 74 29 1 8 81 111 181 1.90e-21 87.4
Msa0780720 MtrunA17_Chr7g0256001 28.758 153 109 0 9 161 349 501 6.22e-21 89.4
Msa0780720 MtrunA17_Chr8g0355131 28.986 138 78 3 17 135 260 396 1.43e-11 62.4
Msa0780720 MtrunA17_Chr1g0203881 45.763 59 32 0 8 66 244 302 4.26e-11 60.8
Msa0780720 MtrunA17_Chr1g0190131 45.161 62 34 0 8 69 209 270 4.67e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0780720 AT5G12180.1 81.132 159 23 1 8 159 276 434 2.70e-87 266
Msa0780720 AT5G19360.1 80.503 159 24 1 8 159 271 429 1.25e-85 261
Msa0780720 AT4G23650.1 65.409 159 48 1 8 159 281 439 8.91e-67 213
Msa0780720 AT3G20410.2 64.151 159 50 1 8 159 294 452 3.49e-65 209
Msa0780720 AT3G20410.1 64.151 159 50 1 8 159 294 452 3.49e-65 209
Msa0780720 AT4G21940.3 65.409 159 47 2 8 159 305 462 1.24e-64 206
Msa0780720 AT1G50700.3 64.151 159 50 1 8 159 276 434 3.06e-64 205
Msa0780720 AT4G21940.1 65.409 159 47 2 8 159 305 462 4.04e-64 207
Msa0780720 AT4G21940.2 65.409 159 47 2 8 159 305 462 5.10e-64 206
Msa0780720 AT1G50700.1 64.151 159 50 1 8 159 276 434 8.41e-64 205
Msa0780720 AT4G04720.2 64.815 162 47 3 7 159 280 440 2.34e-63 204
Msa0780720 AT4G04720.1 64.815 162 47 3 7 159 280 440 2.34e-63 204
Msa0780720 AT3G10660.2 63.522 159 51 1 8 159 389 547 5.07e-62 203
Msa0780720 AT3G10660.1 63.522 159 51 1 8 159 389 547 5.07e-62 203
Msa0780720 AT4G35310.2 60.377 159 56 1 8 159 300 458 1.11e-60 197
Msa0780720 AT4G35310.1 60.377 159 56 1 8 159 300 458 1.11e-60 197
Msa0780720 AT4G38230.1 59.119 159 58 1 8 159 83 241 2.86e-60 191
Msa0780720 AT5G04870.1 61.635 159 54 1 8 159 353 511 3.44e-60 197
Msa0780720 AT2G17290.1 59.748 159 57 1 8 159 288 446 7.80e-60 195
Msa0780720 AT2G17290.2 59.748 159 57 1 8 159 288 446 7.80e-60 195
Msa0780720 AT1G50700.2 55.135 185 50 1 8 159 276 460 9.47e-60 195
Msa0780720 AT1G61950.1 61.006 159 55 1 8 159 302 460 2.07e-59 194
Msa0780720 AT4G38230.4 59.119 159 58 1 8 159 227 385 3.81e-59 192
Msa0780720 AT4G38230.3 59.119 159 58 1 8 159 227 385 3.81e-59 192
Msa0780720 AT4G38230.2 59.119 159 58 1 8 159 257 415 7.09e-59 192
Msa0780720 AT1G76040.1 58.491 159 58 2 8 159 77 234 1.31e-56 181
Msa0780720 AT1G76040.4 58.491 159 58 2 8 159 77 234 1.31e-56 181
Msa0780720 AT2G38910.1 59.119 159 58 1 8 159 337 495 1.42e-56 187
Msa0780720 AT4G09570.1 59.119 159 58 1 8 159 228 386 3.72e-56 184
Msa0780720 AT1G35670.1 59.119 159 58 1 8 159 229 387 7.10e-56 184
Msa0780720 AT4G04740.4 51.000 200 71 6 7 186 269 461 1.98e-55 183
Msa0780720 AT4G04740.6 51.000 200 71 6 7 186 269 461 1.98e-55 183
Msa0780720 AT4G04740.5 51.000 200 71 6 7 186 269 461 1.98e-55 183
Msa0780720 AT4G04740.3 51.000 200 71 6 7 186 269 461 1.98e-55 183
Msa0780720 AT4G04740.1 51.000 200 71 6 7 186 269 461 1.98e-55 183
Msa0780720 AT4G04740.7 51.000 200 71 6 7 186 269 461 1.98e-55 183
Msa0780720 AT4G04740.2 51.000 200 71 6 7 186 269 461 2.05e-55 183
Msa0780720 AT1G76040.3 58.491 159 58 2 8 159 315 472 1.77e-54 180
Msa0780720 AT5G23580.1 57.862 159 60 1 8 159 225 383 2.10e-54 180
Msa0780720 AT1G76040.2 58.491 159 58 2 8 159 315 472 4.61e-54 180
Msa0780720 AT4G04710.3 60.690 145 48 2 22 159 237 379 4.13e-51 171
Msa0780720 AT4G04710.2 60.690 145 48 2 22 159 251 393 4.53e-51 171
Msa0780720 AT4G04695.1 55.975 159 62 2 8 159 235 392 4.95e-51 171
Msa0780720 AT4G04710.1 60.690 145 48 2 22 159 264 406 6.11e-51 171
Msa0780720 AT4G04710.4 59.864 147 48 2 22 159 264 408 1.36e-50 170
Msa0780720 AT4G04700.1 53.459 159 66 2 8 159 235 392 1.12e-48 165
Msa0780720 AT3G51850.2 51.572 159 70 1 8 159 257 415 6.52e-48 163
Msa0780720 AT2G35890.2 50.000 160 72 1 8 159 335 494 1.19e-47 162
Msa0780720 AT2G35890.1 50.000 160 72 1 8 159 335 494 1.19e-47 162
Msa0780720 AT3G51850.1 51.572 159 70 1 8 159 257 415 1.27e-47 162
Msa0780720 AT3G51850.3 54.286 140 64 0 8 147 257 396 3.05e-46 157
Msa0780720 AT1G18890.1 52.201 159 69 1 8 159 266 424 9.36e-46 158
Msa0780720 AT5G12480.2 47.170 159 77 1 8 159 168 326 3.26e-42 147
Msa0780720 AT1G74740.1 49.057 159 74 1 8 159 262 420 6.89e-42 147
Msa0780720 AT5G12480.1 47.170 159 77 1 8 159 262 420 1.27e-41 147
Msa0780720 AT5G19450.2 46.250 160 78 1 8 159 260 419 6.96e-41 145
Msa0780720 AT5G19450.1 46.250 160 78 1 8 159 260 419 6.96e-41 145
Msa0780720 AT2G31500.1 44.025 159 82 1 8 159 269 427 6.05e-40 143
Msa0780720 AT3G57530.1 45.283 159 80 1 8 159 266 424 2.36e-39 140
Msa0780720 AT5G66210.5 41.509 159 85 2 9 159 268 426 2.77e-38 137
Msa0780720 AT5G66210.3 41.509 159 85 2 9 159 268 426 3.77e-38 137
Msa0780720 AT2G41860.2 44.654 159 81 1 8 159 257 415 4.17e-38 137
Msa0780720 AT2G41860.1 44.654 159 81 1 8 159 257 415 4.26e-38 137
Msa0780720 AT5G66210.1 41.509 159 85 2 9 159 268 426 4.66e-38 137
Msa0780720 AT5G66210.2 41.509 159 85 2 9 159 268 426 4.66e-38 137
Msa0780720 AT2G17890.1 39.375 160 89 2 8 159 313 472 4.81e-38 137
Msa0780720 AT3G57530.2 47.482 139 73 0 8 146 266 404 6.02e-38 135
Msa0780720 AT5G66210.6 46.923 130 69 0 8 137 267 396 1.60e-37 134
Msa0780720 AT5G66210.4 46.923 130 69 0 8 137 267 396 1.60e-37 134
Msa0780720 AT4G36070.1 37.423 163 94 2 8 162 276 438 6.78e-35 129
Msa0780720 AT4G36070.2 37.423 163 94 2 8 162 276 438 7.80e-35 129
Msa0780720 AT3G50530.2 34.091 132 86 1 9 140 387 517 3.91e-25 102
Msa0780720 AT3G50530.1 34.091 132 86 1 9 140 356 486 4.01e-25 102
Msa0780720 AT5G24430.1 34.091 132 84 2 9 140 351 479 3.05e-22 93.6
Msa0780720 AT3G19100.1 30.460 174 111 4 9 173 352 524 3.10e-22 93.6
Msa0780720 AT1G49580.1 31.579 133 91 0 8 140 357 489 6.11e-22 92.8
Msa0780720 AT3G49370.1 33.333 132 85 2 9 140 350 478 1.62e-21 91.7
Msa0780720 AT3G56760.1 31.818 132 89 1 9 140 332 462 2.87e-19 85.1
Msa0780720 AT2G41140.1 31.061 132 90 1 9 140 331 461 4.46e-19 84.3
Msa0780720 AT2G46700.2 31.061 132 89 1 9 140 170 299 8.01e-17 77.8
Msa0780720 AT2G46700.3 31.061 132 89 1 9 140 170 299 8.01e-17 77.8
Msa0780720 AT2G46700.1 31.061 132 89 1 9 140 351 480 1.41e-16 77.4
Msa0780720 AT1G12580.1 41.935 62 36 0 8 69 247 308 2.64e-11 62.0

Find 39 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTGAAGCAGATGTTTA+AGG 0.208198 5_2:-62770265 Msa0780720:CDS
AGGTTGGCAATTCACCATTT+TGG 0.280264 5_2:+62769602 None:intergenic
TTAGAGCAAATTTCTTGAAT+TGG 0.309055 5_2:+62770430 None:intergenic
AAAAGTCAACATGTCCTCTT+AGG 0.332441 5_2:+62770769 None:intergenic
ATGAAACTGAAACTAAGATT+TGG 0.353092 5_2:-62770829 Msa0780720:intron
TTGACAATGCAGTGCTCAAT+AGG 0.360502 5_2:-62770476 Msa0780720:CDS
ATTCAAGAAATTTGCTCTAA+AGG 0.373796 5_2:-62770427 Msa0780720:intron
CTTGAGCTCTTCAATTGTAA+TGG 0.375022 5_2:+62770218 None:intergenic
ATCCTTTGCTGAAGGTGAAA+TGG 0.408368 5_2:+62770728 None:intergenic
CATGTTATTCTCTGTTCAAT+CGG 0.411523 5_2:+62769944 None:intergenic
GGATTGAAGCAGATGTTTAA+GGG 0.418191 5_2:-62770264 Msa0780720:CDS
GCATTGTCAAGAGGTGTATC+TGG 0.430236 5_2:+62770489 None:intergenic
GGGCTTGCAAAACAAGGAAC+TGG 0.431849 5_2:-62770192 Msa0780720:CDS
CTTATCAGAGAAAGAAATCA+TGG 0.439000 5_2:-62770286 Msa0780720:CDS
ACAGTCAGCCACCTCCAAAA+TGG 0.448931 5_2:-62769616 Msa0780720:intron
TATGCTGTAATCCTCTGCTT+GGG 0.464432 5_2:+62770678 None:intergenic
ATATGCTGTAATCCTCTGCT+TGG 0.465874 5_2:+62770677 None:intergenic
GAAGCAGATGTTTAAGGGTA+TGG 0.469104 5_2:-62770259 Msa0780720:CDS
CAGGCAGACGCTGATGGTAA+TGG 0.472029 5_2:-62770019 Msa0780720:CDS
CTTCAGCAAAGGATCTTGTA+AGG 0.491058 5_2:-62770719 Msa0780720:CDS
TCTCCGTCTTCCTTGATCCA+CGG 0.508702 5_2:+62770516 None:intergenic
ATATTCAATGCCATCCTAAG+AGG 0.509728 5_2:-62770783 Msa0780720:CDS
CTCCATTTCACCTTCAGCAA+AGG 0.517768 5_2:-62770730 Msa0780720:CDS
AATAGATCATCCGTGGATCA+AGG 0.520654 5_2:-62770526 Msa0780720:intron
CTTACAAGATCCTTTGCTGA+AGG 0.537489 5_2:+62770720 None:intergenic
TGTAATGGTTCCACTGTTGT+CGG 0.558625 5_2:+62770233 None:intergenic
GTCAACATGTCCTCTTAGGA+TGG 0.577946 5_2:+62770773 None:intergenic
ACAATTGAAGAGCTCAAGCA+AGG 0.579738 5_2:-62770213 Msa0780720:CDS
AAGCAAGGGCTTGCAAAACA+AGG 0.587055 5_2:-62770198 Msa0780720:CDS
CAATTGAAGAGCTCAAGCAA+GGG 0.598566 5_2:-62770212 Msa0780720:CDS
TTGAGCACTGCATTGTCAAG+AGG 0.599495 5_2:+62770480 None:intergenic
GAAGGTGAAATGGAGGGCCA+TGG 0.605402 5_2:+62770738 None:intergenic
AAATTACAGGCAGACGCTGA+TGG 0.614845 5_2:-62770025 Msa0780720:intron
CTTTGCTGAAGGTGAAATGG+AGG 0.615396 5_2:+62770731 None:intergenic
CATCCGTGGATCAAGGAAGA+CGG 0.620629 5_2:-62770519 Msa0780720:CDS
TTATCAGAGAAAGAAATCAT+GGG 0.628495 5_2:-62770285 Msa0780720:CDS
TTTGCTGAAGGTGAAATGGA+GGG 0.662601 5_2:+62770732 None:intergenic
TGAATTCAGATCCCAAGCAG+AGG 0.665782 5_2:-62770689 Msa0780720:CDS
GGTATGGACACCGACAACAG+TGG 0.700002 5_2:-62770243 Msa0780720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTAATAAAATTTACATA+TGG - chr5_2:62770102-62770121 Msa0780720:intron 10.0%
!! ATACTAATAAAATTTACATA+TGG - chr5_2:62770102-62770121 Msa0780720:intron 10.0%
!! ATTCAATTATTATGTTTACA+TGG - chr5_2:62769806-62769825 Msa0780720:intron 15.0%
!! AATAAAATTTACATATGGAA+AGG - chr5_2:62770107-62770126 Msa0780720:intron 15.0%
!! ATTCAATTATTATGTTTACA+TGG - chr5_2:62769806-62769825 Msa0780720:intron 15.0%
!! AATAAAATTTACATATGGAA+AGG - chr5_2:62770107-62770126 Msa0780720:intron 15.0%
!! TTTGCTTATAATCAAATCTT+AGG - chr5_2:62770029-62770048 Msa0780720:intron 20.0%
!! AAATCATTAAGTAATCATAG+TGG + chr5_2:62770372-62770391 None:intergenic 20.0%
!! TTTGCTTATAATCAAATCTT+AGG - chr5_2:62770029-62770048 Msa0780720:intron 20.0%
!! AAATCATTAAGTAATCATAG+TGG + chr5_2:62770372-62770391 None:intergenic 20.0%
! ATGAAACTGAAACTAAGATT+TGG - chr5_2:62769600-62769619 Msa0780720:CDS 25.0%
! TTAGAGCAAATTTCTTGAAT+TGG + chr5_2:62770002-62770021 None:intergenic 25.0%
!! ATTCAAGAAATTTGCTCTAA+AGG - chr5_2:62770002-62770021 Msa0780720:CDS 25.0%
! AAGTTCAATACAATCTTAGT+GGG + chr5_2:62770076-62770095 None:intergenic 25.0%
! TAAGTTCAATACAATCTTAG+TGG + chr5_2:62770077-62770096 None:intergenic 25.0%
! TTATCAGAGAAAGAAATCAT+GGG - chr5_2:62770144-62770163 Msa0780720:intron 25.0%
! ACAAGAAGTTAAGCAATTAA+TGG - chr5_2:62770269-62770288 Msa0780720:CDS 25.0%
!!! ATCTTTATCGAAGTATTGAA+AGG + chr5_2:62770520-62770539 None:intergenic 25.0%
! AATGTTAATGTACCTAATCA+CGG + chr5_2:62770651-62770670 None:intergenic 25.0%
! GATTATTCTGACAAGAAAAT+AGG - chr5_2:62770774-62770793 Msa0780720:CDS 25.0%
! ATGAAACTGAAACTAAGATT+TGG - chr5_2:62769600-62769619 Msa0780720:CDS 25.0%
! TTAGAGCAAATTTCTTGAAT+TGG + chr5_2:62770002-62770021 None:intergenic 25.0%
!! ATTCAAGAAATTTGCTCTAA+AGG - chr5_2:62770002-62770021 Msa0780720:CDS 25.0%
! AAGTTCAATACAATCTTAGT+GGG + chr5_2:62770076-62770095 None:intergenic 25.0%
! TAAGTTCAATACAATCTTAG+TGG + chr5_2:62770077-62770096 None:intergenic 25.0%
! TTATCAGAGAAAGAAATCAT+GGG - chr5_2:62770144-62770163 Msa0780720:intron 25.0%
! ACAAGAAGTTAAGCAATTAA+TGG - chr5_2:62770269-62770288 Msa0780720:CDS 25.0%
!!! ATCTTTATCGAAGTATTGAA+AGG + chr5_2:62770520-62770539 None:intergenic 25.0%
! AATGTTAATGTACCTAATCA+CGG + chr5_2:62770651-62770670 None:intergenic 25.0%
! GATTATTCTGACAAGAAAAT+AGG - chr5_2:62770774-62770793 Msa0780720:CDS 25.0%
ATTTCGATTACACCAATTGA+CGG + chr5_2:62769857-62769876 None:intergenic 30.0%
AGTTCAATACAATCTTAGTG+GGG + chr5_2:62770075-62770094 None:intergenic 30.0%
CTTATCAGAGAAAGAAATCA+TGG - chr5_2:62770143-62770162 Msa0780720:intron 30.0%
TTTGTGTATGTGCAAATTAC+AGG - chr5_2:62770391-62770410 Msa0780720:intron 30.0%
CATGTTATTCTCTGTTCAAT+CGG + chr5_2:62770488-62770507 None:intergenic 30.0%
ATAAATCCGTAAAACCAACA+TGG - chr5_2:62770562-62770581 Msa0780720:intron 30.0%
GTAAATATATCCAACCATGT+TGG + chr5_2:62770579-62770598 None:intergenic 30.0%
ATTTCGATTACACCAATTGA+CGG + chr5_2:62769857-62769876 None:intergenic 30.0%
AGTTCAATACAATCTTAGTG+GGG + chr5_2:62770075-62770094 None:intergenic 30.0%
CTTATCAGAGAAAGAAATCA+TGG - chr5_2:62770143-62770162 Msa0780720:intron 30.0%
TTTGTGTATGTGCAAATTAC+AGG - chr5_2:62770391-62770410 Msa0780720:intron 30.0%
CATGTTATTCTCTGTTCAAT+CGG + chr5_2:62770488-62770507 None:intergenic 30.0%
ATAAATCCGTAAAACCAACA+TGG - chr5_2:62770562-62770581 Msa0780720:intron 30.0%
GTAAATATATCCAACCATGT+TGG + chr5_2:62770579-62770598 None:intergenic 30.0%
ATATTCAATGCCATCCTAAG+AGG - chr5_2:62769646-62769665 Msa0780720:intron 35.0%
AAAAGTCAACATGTCCTCTT+AGG + chr5_2:62769663-62769682 None:intergenic 35.0%
!! TTGACTTTTCTAGTGATCCA+TGG - chr5_2:62769674-62769693 Msa0780720:intron 35.0%
GGGTGAAGTCAAGTTAATTT+GGG + chr5_2:62769834-62769853 None:intergenic 35.0%
TTTCGATTACACCAATTGAC+GGG + chr5_2:62769856-62769875 None:intergenic 35.0%
TGTGAAAAATAGATCATCCG+TGG - chr5_2:62769896-62769915 Msa0780720:intron 35.0%
GGATTGAAGCAGATGTTTAA+GGG - chr5_2:62770165-62770184 Msa0780720:intron 35.0%
CTTGAGCTCTTCAATTGTAA+TGG + chr5_2:62770214-62770233 None:intergenic 35.0%
TAACCGAAAAAATCGAAGGA+GGG + chr5_2:62770311-62770330 None:intergenic 35.0%
!! ATAGTGGAGAAGCTTCATTT+TGG + chr5_2:62770356-62770375 None:intergenic 35.0%
ACTTCGATAAAGATAACAGC+AGG - chr5_2:62770526-62770545 Msa0780720:intron 35.0%
!!! ATCCAACCATGTTGGTTTTA+CGG + chr5_2:62770571-62770590 None:intergenic 35.0%
TTGACACAATTAGCATCCAA+AGG + chr5_2:62770606-62770625 None:intergenic 35.0%
ATATTCAATGCCATCCTAAG+AGG - chr5_2:62769646-62769665 Msa0780720:intron 35.0%
AAAAGTCAACATGTCCTCTT+AGG + chr5_2:62769663-62769682 None:intergenic 35.0%
!! TTGACTTTTCTAGTGATCCA+TGG - chr5_2:62769674-62769693 Msa0780720:intron 35.0%
GGGTGAAGTCAAGTTAATTT+GGG + chr5_2:62769834-62769853 None:intergenic 35.0%
TTTCGATTACACCAATTGAC+GGG + chr5_2:62769856-62769875 None:intergenic 35.0%
TGTGAAAAATAGATCATCCG+TGG - chr5_2:62769896-62769915 Msa0780720:intron 35.0%
GGATTGAAGCAGATGTTTAA+GGG - chr5_2:62770165-62770184 Msa0780720:intron 35.0%
CTTGAGCTCTTCAATTGTAA+TGG + chr5_2:62770214-62770233 None:intergenic 35.0%
TAACCGAAAAAATCGAAGGA+GGG + chr5_2:62770311-62770330 None:intergenic 35.0%
!! ATAGTGGAGAAGCTTCATTT+TGG + chr5_2:62770356-62770375 None:intergenic 35.0%
ACTTCGATAAAGATAACAGC+AGG - chr5_2:62770526-62770545 Msa0780720:intron 35.0%
!!! ATCCAACCATGTTGGTTTTA+CGG + chr5_2:62770571-62770590 None:intergenic 35.0%
TTGACACAATTAGCATCCAA+AGG + chr5_2:62770606-62770625 None:intergenic 35.0%
TTTGCTGAAGGTGAAATGGA+GGG + chr5_2:62769700-62769719 None:intergenic 40.0%
ATCCTTTGCTGAAGGTGAAA+TGG + chr5_2:62769704-62769723 None:intergenic 40.0%
CTTACAAGATCCTTTGCTGA+AGG + chr5_2:62769712-62769731 None:intergenic 40.0%
CTTCAGCAAAGGATCTTGTA+AGG - chr5_2:62769710-62769729 Msa0780720:intron 40.0%
TATGCTGTAATCCTCTGCTT+GGG + chr5_2:62769754-62769773 None:intergenic 40.0%
ATATGCTGTAATCCTCTGCT+TGG + chr5_2:62769755-62769774 None:intergenic 40.0%
GGGGTGAAGTCAAGTTAATT+TGG + chr5_2:62769835-62769854 None:intergenic 40.0%
TTCGATTACACCAATTGACG+GGG + chr5_2:62769855-62769874 None:intergenic 40.0%
AATAGATCATCCGTGGATCA+AGG - chr5_2:62769903-62769922 Msa0780720:intron 40.0%
TTGACAATGCAGTGCTCAAT+AGG - chr5_2:62769953-62769972 Msa0780720:CDS 40.0%
GGGATTGAAGCAGATGTTTA+AGG - chr5_2:62770164-62770183 Msa0780720:intron 40.0%
GAAGCAGATGTTTAAGGGTA+TGG - chr5_2:62770170-62770189 Msa0780720:intron 40.0%
TGTAATGGTTCCACTGTTGT+CGG + chr5_2:62770199-62770218 None:intergenic 40.0%
ACAATTGAAGAGCTCAAGCA+AGG - chr5_2:62770216-62770235 Msa0780720:CDS 40.0%
CAATTGAAGAGCTCAAGCAA+GGG - chr5_2:62770217-62770236 Msa0780720:CDS 40.0%
!!! TCTCCCTCCTTCGATTTTTT+CGG - chr5_2:62770305-62770324 Msa0780720:intron 40.0%
GTAACCGAAAAAATCGAAGG+AGG + chr5_2:62770312-62770331 None:intergenic 40.0%
GCAGTAACCGAAAAAATCGA+AGG + chr5_2:62770315-62770334 None:intergenic 40.0%
!!! CGATTTTTTCGGTTACTGCA+TGG - chr5_2:62770316-62770335 Msa0780720:intron 40.0%
ATCCGTAAAACCAACATGGT+TGG - chr5_2:62770566-62770585 Msa0780720:intron 40.0%
GGATATATTTACCCCTCCTT+TGG - chr5_2:62770587-62770606 Msa0780720:intron 40.0%
ACAATTAGCATCCAAAGGAG+GGG + chr5_2:62770601-62770620 None:intergenic 40.0%
CACAATTAGCATCCAAAGGA+GGG + chr5_2:62770602-62770621 None:intergenic 40.0%
ACACAATTAGCATCCAAAGG+AGG + chr5_2:62770603-62770622 None:intergenic 40.0%
!!! CTCACTGATTTTCCGTGATT+AGG - chr5_2:62770636-62770655 Msa0780720:intron 40.0%
! TTGGCAATTCACCATTTTGG+AGG + chr5_2:62770827-62770846 None:intergenic 40.0%
! AGGTTGGCAATTCACCATTT+TGG + chr5_2:62770830-62770849 None:intergenic 40.0%
TTTGCTGAAGGTGAAATGGA+GGG + chr5_2:62769700-62769719 None:intergenic 40.0%
ATCCTTTGCTGAAGGTGAAA+TGG + chr5_2:62769704-62769723 None:intergenic 40.0%
CTTACAAGATCCTTTGCTGA+AGG + chr5_2:62769712-62769731 None:intergenic 40.0%
CTTCAGCAAAGGATCTTGTA+AGG - chr5_2:62769710-62769729 Msa0780720:intron 40.0%
TATGCTGTAATCCTCTGCTT+GGG + chr5_2:62769754-62769773 None:intergenic 40.0%
ATATGCTGTAATCCTCTGCT+TGG + chr5_2:62769755-62769774 None:intergenic 40.0%
GGGGTGAAGTCAAGTTAATT+TGG + chr5_2:62769835-62769854 None:intergenic 40.0%
TTCGATTACACCAATTGACG+GGG + chr5_2:62769855-62769874 None:intergenic 40.0%
AATAGATCATCCGTGGATCA+AGG - chr5_2:62769903-62769922 Msa0780720:intron 40.0%
TTGACAATGCAGTGCTCAAT+AGG - chr5_2:62769953-62769972 Msa0780720:CDS 40.0%
GGGATTGAAGCAGATGTTTA+AGG - chr5_2:62770164-62770183 Msa0780720:intron 40.0%
GAAGCAGATGTTTAAGGGTA+TGG - chr5_2:62770170-62770189 Msa0780720:intron 40.0%
TGTAATGGTTCCACTGTTGT+CGG + chr5_2:62770199-62770218 None:intergenic 40.0%
ACAATTGAAGAGCTCAAGCA+AGG - chr5_2:62770216-62770235 Msa0780720:CDS 40.0%
CAATTGAAGAGCTCAAGCAA+GGG - chr5_2:62770217-62770236 Msa0780720:CDS 40.0%
!!! TCTCCCTCCTTCGATTTTTT+CGG - chr5_2:62770305-62770324 Msa0780720:intron 40.0%
GTAACCGAAAAAATCGAAGG+AGG + chr5_2:62770312-62770331 None:intergenic 40.0%
GCAGTAACCGAAAAAATCGA+AGG + chr5_2:62770315-62770334 None:intergenic 40.0%
!!! CGATTTTTTCGGTTACTGCA+TGG - chr5_2:62770316-62770335 Msa0780720:intron 40.0%
ATCCGTAAAACCAACATGGT+TGG - chr5_2:62770566-62770585 Msa0780720:intron 40.0%
GGATATATTTACCCCTCCTT+TGG - chr5_2:62770587-62770606 Msa0780720:intron 40.0%
ACAATTAGCATCCAAAGGAG+GGG + chr5_2:62770601-62770620 None:intergenic 40.0%
CACAATTAGCATCCAAAGGA+GGG + chr5_2:62770602-62770621 None:intergenic 40.0%
ACACAATTAGCATCCAAAGG+AGG + chr5_2:62770603-62770622 None:intergenic 40.0%
!!! CTCACTGATTTTCCGTGATT+AGG - chr5_2:62770636-62770655 Msa0780720:intron 40.0%
! TTGGCAATTCACCATTTTGG+AGG + chr5_2:62770827-62770846 None:intergenic 40.0%
! AGGTTGGCAATTCACCATTT+TGG + chr5_2:62770830-62770849 None:intergenic 40.0%
GTCAACATGTCCTCTTAGGA+TGG + chr5_2:62769659-62769678 None:intergenic 45.0%
CTTTGCTGAAGGTGAAATGG+AGG + chr5_2:62769701-62769720 None:intergenic 45.0%
CTCCATTTCACCTTCAGCAA+AGG - chr5_2:62769699-62769718 Msa0780720:intron 45.0%
TGAATTCAGATCCCAAGCAG+AGG - chr5_2:62769740-62769759 Msa0780720:intron 45.0%
TCGATTACACCAATTGACGG+GGG + chr5_2:62769854-62769873 None:intergenic 45.0%
! GCATTGTCAAGAGGTGTATC+TGG + chr5_2:62769943-62769962 None:intergenic 45.0%
! TTGAGCACTGCATTGTCAAG+AGG + chr5_2:62769952-62769971 None:intergenic 45.0%
AAGCAAGGGCTTGCAAAACA+AGG - chr5_2:62770231-62770250 Msa0780720:CDS 45.0%
AAATTACAGGCAGACGCTGA+TGG - chr5_2:62770404-62770423 Msa0780720:intron 45.0%
!! GCAATTCACCATTTTGGAGG+TGG + chr5_2:62770824-62770843 None:intergenic 45.0%
GTCAACATGTCCTCTTAGGA+TGG + chr5_2:62769659-62769678 None:intergenic 45.0%
CTTTGCTGAAGGTGAAATGG+AGG + chr5_2:62769701-62769720 None:intergenic 45.0%
CTCCATTTCACCTTCAGCAA+AGG - chr5_2:62769699-62769718 Msa0780720:intron 45.0%
TGAATTCAGATCCCAAGCAG+AGG - chr5_2:62769740-62769759 Msa0780720:intron 45.0%
TCGATTACACCAATTGACGG+GGG + chr5_2:62769854-62769873 None:intergenic 45.0%
! GCATTGTCAAGAGGTGTATC+TGG + chr5_2:62769943-62769962 None:intergenic 45.0%
! TTGAGCACTGCATTGTCAAG+AGG + chr5_2:62769952-62769971 None:intergenic 45.0%
AAGCAAGGGCTTGCAAAACA+AGG - chr5_2:62770231-62770250 Msa0780720:CDS 45.0%
AAATTACAGGCAGACGCTGA+TGG - chr5_2:62770404-62770423 Msa0780720:intron 45.0%
!! GCAATTCACCATTTTGGAGG+TGG + chr5_2:62770824-62770843 None:intergenic 45.0%
TTGACTTCACCCCCGTCAAT+TGG - chr5_2:62769842-62769861 Msa0780720:intron 50.0%
CATCCGTGGATCAAGGAAGA+CGG - chr5_2:62769910-62769929 Msa0780720:intron 50.0%
TCTCCGTCTTCCTTGATCCA+CGG + chr5_2:62769916-62769935 None:intergenic 50.0%
GGGCTTGCAAAACAAGGAAC+TGG - chr5_2:62770237-62770256 Msa0780720:CDS 50.0%
ACAGTCAGCCACCTCCAAAA+TGG - chr5_2:62770813-62770832 Msa0780720:intron 50.0%
TTGACTTCACCCCCGTCAAT+TGG - chr5_2:62769842-62769861 Msa0780720:intron 50.0%
CATCCGTGGATCAAGGAAGA+CGG - chr5_2:62769910-62769929 Msa0780720:intron 50.0%
TCTCCGTCTTCCTTGATCCA+CGG + chr5_2:62769916-62769935 None:intergenic 50.0%
GGGCTTGCAAAACAAGGAAC+TGG - chr5_2:62770237-62770256 Msa0780720:CDS 50.0%
ACAGTCAGCCACCTCCAAAA+TGG - chr5_2:62770813-62770832 Msa0780720:intron 50.0%
GAAGGTGAAATGGAGGGCCA+TGG + chr5_2:62769694-62769713 None:intergenic 55.0%
GGTATGGACACCGACAACAG+TGG - chr5_2:62770186-62770205 Msa0780720:CDS 55.0%
CAGGCAGACGCTGATGGTAA+TGG - chr5_2:62770410-62770429 Msa0780720:intron 55.0%
GAAGGTGAAATGGAGGGCCA+TGG + chr5_2:62769694-62769713 None:intergenic 55.0%
GGTATGGACACCGACAACAG+TGG - chr5_2:62770186-62770205 Msa0780720:CDS 55.0%
CAGGCAGACGCTGATGGTAA+TGG - chr5_2:62770410-62770429 Msa0780720:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 62769600 62770851 62769600 ID=Msa0780720;Name=Msa0780720
chr5_2 mRNA 62769600 62770851 62769600 ID=Msa0780720-mRNA-1;Parent=Msa0780720;Name=Msa0780720-mRNA-1;_AED=0.28;_eAED=0.49;_QI=0|0|0|0.66|0.4|0.5|6|0|188
chr5_2 exon 62770830 62770851 62770830 ID=Msa0780720-mRNA-1:exon:18590;Parent=Msa0780720-mRNA-1
chr5_2 exon 62770667 62770819 62770667 ID=Msa0780720-mRNA-1:exon:18589;Parent=Msa0780720-mRNA-1
chr5_2 exon 62770428 62770543 62770428 ID=Msa0780720-mRNA-1:exon:18588;Parent=Msa0780720-mRNA-1
chr5_2 exon 62770176 62770322 62770176 ID=Msa0780720-mRNA-1:exon:18587;Parent=Msa0780720-mRNA-1
chr5_2 exon 62769945 62770038 62769945 ID=Msa0780720-mRNA-1:exon:18586;Parent=Msa0780720-mRNA-1
chr5_2 exon 62769600 62769634 62769600 ID=Msa0780720-mRNA-1:exon:18585;Parent=Msa0780720-mRNA-1
chr5_2 CDS 62770830 62770851 62770830 ID=Msa0780720-mRNA-1:cds;Parent=Msa0780720-mRNA-1
chr5_2 CDS 62770667 62770819 62770667 ID=Msa0780720-mRNA-1:cds;Parent=Msa0780720-mRNA-1
chr5_2 CDS 62770428 62770543 62770428 ID=Msa0780720-mRNA-1:cds;Parent=Msa0780720-mRNA-1
chr5_2 CDS 62770176 62770322 62770176 ID=Msa0780720-mRNA-1:cds;Parent=Msa0780720-mRNA-1
chr5_2 CDS 62769945 62770038 62769945 ID=Msa0780720-mRNA-1:cds;Parent=Msa0780720-mRNA-1
chr5_2 CDS 62769600 62769634 62769600 ID=Msa0780720-mRNA-1:cds;Parent=Msa0780720-mRNA-1
Gene Sequence

>Msa0780720

ATGAAACTGAAACTAAGATTTGAATCTGAAAATGAGATATTCAATGCCATCCTAAGAGGACATGTTGACTTTTCTAGTGATCCATGGCCCTCCATTTCACCTTCAGCAAAGGATCTTGTAAGGAAAATGTTGAATTCAGATCCCAAGCAGAGGATTACAGCATATGAAGTTCTGAATCATCCGTGGATCAAGGAAGACGGAGAAGCACCAGATACACCTCTTGACAATGCAGTGCTCAATAGGCTCAAACAGTTTAGAGCAATGAACCAATTCAAGAAATTTGCTCTAAAGGTTATTGCTAGCTGCTTATCAGAGAAAGAAATCATGGGATTGAAGCAGATGTTTAAGGGTATGGACACCGACAACAGTGGAACCATTACAATTGAAGAGCTCAAGCAAGGGCTTGCAAAACAAGGAACTGGACTGAGTGAACAAGAAGCAGACGCTGATGGTAATGGAATCATTGACTACGACGAGTGTAGAGTTCATCACAGCAACTATGCACATGAACCGATTGAACAGAGAATAACATTCAGCCACCTCCAAAATGGTGAATTGCCAACCTAA
Protein sequence

>Msa0780720

MKLKLRFESENEIFNAILRGHVDFSSDPWPSISPSAKDLVRKMLNSDPKQRITAYEVLNHPWIKEDGEAPDTPLDNAVLNRLKQFRAMNQFKKFALKVIASCLSEKEIMGLKQMFKGMDTDNSGTITIEELKQGLAKQGTGLSEQEADADGNGIIDYDECRVHHSNYAHEPIEQRITFSHLQNGELPT*