Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0781110 | XP_003617168.2 | 44.023 | 343 | 118 | 6 | 13 | 332 | 8 | 299 | 1.55e-61 | 223 |
| Msa0781110 | XP_003617168.2 | 81.270 | 315 | 19 | 3 | 333 | 607 | 14 | 328 | 4.04e-161 | 484 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0781110 | sp|Q9SM27|MY104_ARATH | 50.000 | 132 | 65 | 1 | 329 | 460 | 17 | 147 | 3.02e-34 | 139 |
| Msa0781110 | sp|Q9SM27|MY104_ARATH | 32.955 | 264 | 138 | 6 | 1 | 246 | 3 | 245 | 2.44e-29 | 124 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0781110 | MtrunA17_Chr5g0441131 | 96.026 | 151 | 6 | 0 | 333 | 483 | 14 | 164 | 1.93e-98 | 305 |
| Msa0781110 | MtrunA17_Chr5g0441131 | 50.000 | 206 | 56 | 4 | 13 | 199 | 8 | 185 | 2.12e-47 | 167 |
| Msa0781110 | MtrunA17_Chr5g0440771 | 62.500 | 208 | 56 | 4 | 624 | 823 | 3 | 196 | 4.66e-69 | 227 |
| Msa0781110 | MtrunA17_Chr5g0440761 | 53.909 | 243 | 71 | 7 | 332 | 546 | 16 | 245 | 9.80e-67 | 223 |
| Msa0781110 | MtrunA17_Chr5g0440761 | 44.762 | 315 | 63 | 10 | 15 | 301 | 13 | 244 | 1.76e-60 | 206 |
| Msa0781110 | MtrunA17_Chr5g0441141 | 49.451 | 273 | 89 | 8 | 332 | 568 | 16 | 275 | 9.88e-66 | 221 |
| Msa0781110 | MtrunA17_Chr5g0441141 | 43.064 | 346 | 76 | 12 | 15 | 322 | 13 | 275 | 5.17e-61 | 208 |
| Msa0781110 | MtrunA17_Chr5g0440781 | 71.034 | 145 | 39 | 1 | 332 | 476 | 16 | 157 | 2.01e-63 | 211 |
| Msa0781110 | MtrunA17_Chr5g0440781 | 55.024 | 209 | 38 | 6 | 15 | 199 | 13 | 189 | 9.56e-56 | 190 |
| Msa0781110 | MtrunA17_Chr5g0428071 | 50.254 | 197 | 62 | 8 | 621 | 788 | 39 | 228 | 1.36e-41 | 157 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0781110 | AT2G26950.1 | 50.000 | 132 | 65 | 1 | 329 | 460 | 17 | 147 | 3.07e-35 | 139 |
| Msa0781110 | AT2G26950.1 | 32.955 | 264 | 138 | 6 | 1 | 246 | 3 | 245 | 2.48e-30 | 124 |
| Msa0781110 | AT2G26960.1 | 41.558 | 154 | 71 | 2 | 4 | 139 | 10 | 162 | 2.78e-33 | 134 |
| Msa0781110 | AT2G26960.1 | 48.276 | 116 | 59 | 1 | 329 | 444 | 21 | 135 | 1.43e-31 | 129 |
| Msa0781110 | AT5G55020.1 | 52.419 | 124 | 38 | 2 | 6 | 111 | 21 | 141 | 5.56e-33 | 135 |
| Msa0781110 | AT5G55020.1 | 51.376 | 109 | 52 | 1 | 333 | 441 | 31 | 138 | 8.32e-32 | 131 |
| Msa0781110 | AT4G26930.1 | 46.923 | 130 | 51 | 1 | 4 | 115 | 9 | 138 | 1.12e-32 | 131 |
| Msa0781110 | AT4G26930.1 | 48.624 | 109 | 55 | 1 | 333 | 441 | 24 | 131 | 4.28e-31 | 127 |
| Msa0781110 | AT3G11440.2 | 52.500 | 120 | 39 | 1 | 14 | 115 | 41 | 160 | 5.20e-32 | 131 |
| Msa0781110 | AT3G11440.2 | 48.276 | 116 | 59 | 1 | 333 | 448 | 46 | 160 | 1.01e-31 | 130 |
Find 162 sgRNAs with CRISPR-Local
Find 1216 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATGGAACCAGAAATT+GGG | 0.497571 | 5_2:-63528606 | Msa0781110:CDS |
| AAAAGAAAACTTGAAAAGTG+TGG | 0.603714 | 5_2:-63517963 | Msa0781110:CDS |
| AAAAGAAAACTTGAAAGACA+TGG | 0.648245 | 5_2:-63535565 | Msa0781110:CDS |
| AAACATGATGATAAGTTGAA+TGG | 0.487343 | 5_2:-63535517 | Msa0781110:intron |
| AAACTTATAGTTAGTTATGG+TGG | 0.525984 | 5_2:-63521835 | Msa0781110:CDS |
| AAAGTTCTTGATGTCATTAT+CGG | 0.301665 | 5_2:+63517998 | None:intergenic |
| AAAGTTCTTGATGTCATTAT+CGG | 0.301665 | 5_2:+63528077 | None:intergenic |
| AAATATGCTATACAATAATG+TGG | 0.534435 | 5_2:-63535314 | Msa0781110:CDS |
| AAATCATGCTCCAAAGAAAC+TGG | 0.398721 | 5_2:+63527332 | None:intergenic |
| AAATTTGATACTACGATCAA+CGG | 0.606604 | 5_2:+63521735 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGCAATATCAAAAAAGGC+AGG | - | chr5_2:63517994-63518013 | Msa0781110:CDS | 30.0% | |
| ATGCAATATCAAAAAAGGCA+GGG | - | chr5_2:63517995-63518014 | Msa0781110:CDS | 30.0% | |
| CCTACAACCAGATTTAAAAA+AGG | - | chr5_2:63518062-63518081 | Msa0781110:intron | 30.0% | |
| AAGCATGAATAGAAAAACCT+GGG | + | chr5_2:63518169-63518188 | None:intergenic | 30.0% | |
| ACTGTAAAACTTCATTGTGA+AGG | - | chr5_2:63518518-63518537 | Msa0781110:intron | 30.0% | |
| TAACCATGCCTACAAAATTA+AGG | - | chr5_2:63518555-63518574 | Msa0781110:intron | 30.0% | |
| AGCGTCAAAAAACAAAACAA+CGG | + | chr5_2:63518647-63518666 | None:intergenic | 30.0% | |
| CAATATGGTATAACACAAAG+TGG | + | chr5_2:63519476-63519495 | None:intergenic | 30.0% | |
| CACTTTGTGTTATACCATAT+TGG | - | chr5_2:63519474-63519493 | Msa0781110:intron | 30.0% | |
| AGCTACATTTATCTACTTCA+GGG | - | chr5_2:63520222-63520241 | Msa0781110:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 63517877 | 63537313 | 63517877 | ID=Msa0781110;Name=Msa0781110 |
| chr5_2 | mRNA | 63517877 | 63537313 | 63517877 | ID=Msa0781110-mRNA-1;Parent=Msa0781110;Name=Msa0781110-mRNA-1;_AED=0.16;_eAED=0.19;_QI=0|0|0|1|0.15|0.07|14|0|887 |
| chr5_2 | exon | 63537077 | 63537313 | 63537077 | ID=Msa0781110-mRNA-1:exon:18877;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63535518 | 63535635 | 63535518 | ID=Msa0781110-mRNA-1:exon:18876;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63535268 | 63535508 | 63535268 | ID=Msa0781110-mRNA-1:exon:18875;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63534757 | 63534947 | 63534757 | ID=Msa0781110-mRNA-1:exon:18874;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63534506 | 63534714 | 63534506 | ID=Msa0781110-mRNA-1:exon:18873;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63528430 | 63528669 | 63528430 | ID=Msa0781110-mRNA-1:exon:18872;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63527901 | 63528112 | 63527901 | ID=Msa0781110-mRNA-1:exon:18871;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63527582 | 63527718 | 63527582 | ID=Msa0781110-mRNA-1:exon:18870;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63527292 | 63527527 | 63527292 | ID=Msa0781110-mRNA-1:exon:18869;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63521723 | 63521911 | 63521723 | ID=Msa0781110-mRNA-1:exon:18868;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63521596 | 63521716 | 63521596 | ID=Msa0781110-mRNA-1:exon:18867;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63521345 | 63521577 | 63521345 | ID=Msa0781110-mRNA-1:exon:18866;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63521187 | 63521329 | 63521187 | ID=Msa0781110-mRNA-1:exon:18865;Parent=Msa0781110-mRNA-1 |
| chr5_2 | exon | 63517877 | 63518033 | 63517877 | ID=Msa0781110-mRNA-1:exon:18864;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63537077 | 63537313 | 63537077 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63535518 | 63535635 | 63535518 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63535268 | 63535508 | 63535268 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63534757 | 63534947 | 63534757 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63534506 | 63534714 | 63534506 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63528430 | 63528669 | 63528430 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63527901 | 63528112 | 63527901 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63527582 | 63527718 | 63527582 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63527292 | 63527527 | 63527292 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63521723 | 63521911 | 63521723 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63521596 | 63521716 | 63521596 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63521345 | 63521577 | 63521345 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63521187 | 63521329 | 63521187 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| chr5_2 | CDS | 63517877 | 63518033 | 63517877 | ID=Msa0781110-mRNA-1:cds;Parent=Msa0781110-mRNA-1 |
| Gene Sequence |
>Msa0781110 CAAGAAAAAGCCAATGACGGTGGTGATATGAATGATGAGTTGAAAAAAGGAACATGGTCAAAAGATGAGGATGAAATATTAGAAGCATATTTGACAAAATATGGAGTCGGAAATTGGAATGCAATATCAAAAAAGGCAGGGCTTAAACGTTGTGGAAAAAGTTGTCGTTTTAGATGGTATAATCACCTACAACCAGATTTAAAAAAGGGACCATTTAGTGAAGAAGAAGAAAAAAAATTGCCTGGAAGAACAGATAATGCCATAAAAAACTTTTGGAATGCAAGAAAAAGAAAACTTGAAAGACATGGCTTGTCACCCTACCCTCCTAGCATAAAACATGATGATAAGTTGAATGAAAGTAGTTTGAGTGGTTCTAGCAGCCAACAAGTTGAGGTGAAATTTGGAAAAGATTTTTTGGGTAGAAAATATCCATCAATTTGTGATGAGATAAATGAAAACAACGTTGAAAAACGTTCAAACTTATATGATGTCCCGAAAAATCAGCTTCCAAATTTTTCTGATGATTCAAATATGCTATACAATAATGTGGGAAGTACTTCAATAGGCAATCATACGACCCCAACTTTGATTGACAGGGGTGGCGTTGAACCTTTTCGTATACCTATGGCTATGGTATCACATCTTCCTCCACTCCCACCATGTTCATACATCCAAAATACGAACTATCAACTTCCTTCATATACGATGTCTAGTGAGTATCCGAAAAATCCACCACCTAAGGAGACTCAATATCATATGCCAGAATCAATTGCTCCACATAGTAGTGGCTCAGATGCTAGTCGAACACATTCCTCTGAGATAAATCTAGTTGATAAAAAAGGGAAGAGGCCAATTTCGTTCGAGAATAATGTTGGAGAAAACCGTAGCAACTTTATAGATTTGGATCTTTTGTTTGACTCAAGTTCATTAGATAATGAAAATAACAATAACTTGGATGACAAGGTTCCACACGATATAAAGGATACCTTAGATGCATGGTCAAAAGAAGAGGATGAAATATTAAAAGCATATGTGGAAAAACATGGAACCAGAAATTGGGACGAGGTATCAAAGAACACGGGACTTGCACACTTTGGAAGAAGTTGCCGTTTTAGATGGTATAATAACCTACAACCTGGTGTAAAAAAGGGTCCATTTAGTAAAGAAGAAGAAAATAAAGTCTTTCAGTTACGTAAGGAGTTTGGTGGATTCAAATGGTCTAAAATCGCTCGCGAGTTGCCAGGAAGAACCGATAATGACATCAAGAACTTTTGGAATGCGAGAAAGAGAAAACATGAAAAGCGTGGCTTGTCACCCTTCCCTAACAACATGGAACTGGATGATGAGTTGAATGGTGGTTCCAGTGGCCAACAAGTTGAGGATTCTCAAGAAAATAATTTTGATGTCTCAAATAAGCGGTTTTCAAAGTTGGTTGATGATTCAAATATGGGCGGCGCTGGAATTTCACCTATACTTTTGACTATGCAATCTGATCTGCCTCCACTCCCTCGTAATCCACCACCTATGGAGACTCAATATCAGATGTCAGAATCAATTTCTCCATATACAGAAGAGAATTGGGAAGGTAAGAAGCCAATTTCTTTGGAGAATGATGTTGAAGACCATGACGACTTAATTGCTATAGATATTTGGTTAAACTATGACTTAAATGAAAAAGACATTCACAACTTACATGACAAGCTTCAGCATGATGTAAATGATTCCTTGTACACAACTATGTATGGAAAAGGGGAGGAGCCAGTTTCTTTGGAGCATGATTTTGAAGTAAACCATGACGACTTAATGAATCCGAAACTTTGGTCTCAACACACTTCTAAATTCTTCCACATGGAGTACCCAAACTGTTCGAGAGTCAAACTTATAGTTAGTTATGGTGGAAAAATTGAACCTAGGCTTACCGATCTTCTTCATCATCGTCACTATTTCTACATCGGCGGCGACAATAAAATCATCACCGTTGATCGTAGTATCAAATTTACTAATCTCATGCTTTCTACGTTGACGGTTTCCGATGTTTGCTTCAAATATCAACTCCTTGGTGAATATCTCAATTCTTTGATCCCGGTCGATAACGAAGAAGATCTCGATCATATGATGTTCGAGTACGATCCAAAGCCTGCAAGGTTAAGGGTCTTCCTCTTCCCTGTCCATGTTGACAACAACAAGGCATCTTCTGATTCTTTGGCTTCTACTATCAAGCTCAAACAGCTGTCTGTCAACTCGCTCAATTCCATTCATGTTCCTCCATTAGAAGATTCATCTCCTCCTACTACACCTCCGCCATCGACGAGGAGTGAGATTGAATTCCCGAAGACTTCTCCTGATTTTGCGACTAAGGATACGATGAAGCAATTTCATGGACTACATATCGTGAATGATGAATTTGAAGATATTCTAAAACTGTTTTTGAAGCGTAATTTTTCTAAGAAGAAGGAATTTCATGGACTACACGTCGTAAACGATGAATTTGAAGATATTATAAAACATTGCCTGGAAGAACCGATAATGACATCAAGAACTTTTGGAATGCGAGAAAAAGAAAACTTGAAAAGTGTGGCTTGTTTGTCACCCTTCCCTGACAACATGGAAATGGATGATGAGTTGAATAGTGGTTCCAGTGGCCAACAAGTTGAGGATAAATGA |
| Protein sequence |
>Msa0781110 QEKANDGGDMNDELKKGTWSKDEDEILEAYLTKYGVGNWNAISKKAGLKRCGKSCRFRWYNHLQPDLKKGPFSEEEEKKLPGRTDNAIKNFWNARKRKLERHGLSPYPPSIKHDDKLNESSLSGSSSQQVEVKFGKDFLGRKYPSICDEINENNVEKRSNLYDVPKNQLPNFSDDSNMLYNNVGSTSIGNHTTPTLIDRGGVEPFRIPMAMVSHLPPLPPCSYIQNTNYQLPSYTMSSEYPKNPPPKETQYHMPESIAPHSSGSDASRTHSSEINLVDKKGKRPISFENNVGENRSNFIDLDLLFDSSSLDNENNNNLDDKVPHDIKDTLDAWSKEEDEILKAYVEKHGTRNWDEVSKNTGLAHFGRSCRFRWYNNLQPGVKKGPFSKEEENKVFQLRKEFGGFKWSKIARELPGRTDNDIKNFWNARKRKHEKRGLSPFPNNMELDDELNGGSSGQQVEDSQENNFDVSNKRFSKLVDDSNMGGAGISPILLTMQSDLPPLPRNPPPMETQYQMSESISPYTEENWEGKKPISLENDVEDHDDLIAIDIWLNYDLNEKDIHNLHDKLQHDVNDSLYTTMYGKGEEPVSLEHDFEVNHDDLMNPKLWSQHTSKFFHMEYPNCSRVKLIVSYGGKIEPRLTDLLHHRHYFYIGGDNKIITVDRSIKFTNLMLSTLTVSDVCFKYQLLGEYLNSLIPVDNEEDLDHMMFEYDPKPARLRVFLFPVHVDNNKASSDSLASTIKLKQLSVNSLNSIHVPPLEDSSPPTTPPPSTRSEIEFPKTSPDFATKDTMKQFHGLHIVNDEFEDILKLFLKRNFSKKKEFHGLHVVNDEFEDIIKHCLEEPIMTSRTFGMREKENLKSVACLSPFPDNMEMDDELNSGSSGQQVEDK* |
