Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0781610 | sp|Q8S9H7|DIV_ANTMA | 63.014 | 73 | 23 | 2 | 442 | 513 | 134 | 203 | 1.25e-21 | 99.4 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0781610 | MtrunA17_Chr5g0440741 | 90.323 | 279 | 22 | 2 | 355 | 632 | 15 | 289 | 3.45e-171 | 489 |
| Msa0781610 | MtrunA17_Chr5g0440731 | 89.085 | 284 | 21 | 4 | 355 | 632 | 15 | 294 | 1.19e-166 | 477 |
| Msa0781610 | MtrunA17_Chr5g0440751 | 90.857 | 175 | 16 | 0 | 1 | 175 | 1 | 175 | 4.87e-105 | 316 |
| Msa0781610 | MtrunA17_Chr5g0440751 | 70.556 | 180 | 18 | 3 | 176 | 354 | 17 | 162 | 2.13e-69 | 223 |
| Msa0781610 | MtrunA17_Chr5g0409841 | 65.957 | 235 | 65 | 7 | 357 | 580 | 1 | 231 | 2.40e-85 | 266 |
| Msa0781610 | MtrunA17_Chr5g0440721 | 82.209 | 163 | 16 | 1 | 5 | 167 | 12 | 161 | 6.66e-85 | 263 |
| Msa0781610 | MtrunA17_Chr5g0440721 | 65.000 | 180 | 15 | 3 | 176 | 354 | 24 | 156 | 1.84e-62 | 204 |
| Msa0781610 | MtrunA17_Chr5g0440821 | 72.038 | 211 | 15 | 3 | 9 | 175 | 23 | 233 | 3.05e-83 | 280 |
| Msa0781610 | MtrunA17_Chr5g0409951 | 64.417 | 163 | 24 | 3 | 176 | 329 | 113 | 250 | 4.46e-54 | 185 |
| Msa0781610 | MtrunA17_Chr5g0409951 | 61.842 | 152 | 41 | 2 | 3 | 137 | 99 | 250 | 6.16e-45 | 160 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0781610 | AT5G58900.1 | 41.176 | 136 | 64 | 3 | 442 | 572 | 143 | 267 | 2.51e-23 | 100 |
| Msa0781610 | AT5G08520.1 | 56.790 | 81 | 31 | 2 | 438 | 517 | 115 | 192 | 1.88e-22 | 98.6 |
| Msa0781610 | AT5G04760.1 | 60.526 | 76 | 27 | 1 | 433 | 508 | 92 | 164 | 2.52e-22 | 96.3 |
| Msa0781610 | AT5G01200.1 | 61.194 | 67 | 23 | 1 | 442 | 508 | 148 | 211 | 4.40e-21 | 94.0 |
| Msa0781610 | AT2G38090.1 | 57.692 | 78 | 28 | 3 | 435 | 510 | 135 | 209 | 1.92e-20 | 92.8 |
| Msa0781610 | AT1G49010.1 | 53.659 | 82 | 33 | 3 | 438 | 517 | 131 | 209 | 5.57e-20 | 91.7 |
| Msa0781610 | AT5G61620.1 | 43.651 | 126 | 51 | 5 | 434 | 551 | 101 | 214 | 4.17e-19 | 89.4 |
| Msa0781610 | AT3G11280.2 | 43.443 | 122 | 50 | 5 | 442 | 552 | 129 | 242 | 2.56e-18 | 85.9 |
| Msa0781610 | AT3G11280.1 | 43.443 | 122 | 50 | 5 | 442 | 552 | 129 | 242 | 2.56e-18 | 85.9 |
| Msa0781610 | AT5G23650.1 | 52.632 | 76 | 33 | 1 | 442 | 517 | 122 | 194 | 4.95e-18 | 86.3 |
Find 179 sgRNAs with CRISPR-Local
Find 249 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAGAAATCAAACGGTTC+TGG | 0.380690 | 5_2:+64172116 | Msa0781610:intron |
| AAACCTTGTCCTGCTGCAGC+CGG | 0.478250 | 5_2:-64173228 | None:intergenic |
| AAACCTTGTCCTGCTGCTGC+CGG | 0.447814 | 5_2:-64172281 | None:intergenic |
| AAAGTCATGCCCCAAGGAAA+GGG | 0.493395 | 5_2:+64174124 | Msa0781610:CDS |
| AAATATTTCAAAAGAATTTG+TGG | 0.425853 | 5_2:+64174432 | Msa0781610:CDS |
| AACAAACGAACCTGAACAGA+AGG | 0.557127 | 5_2:-64172587 | None:intergenic |
| AACAATCGAACCTGAACAGA+AGG | 0.565912 | 5_2:-64171734 | None:intergenic |
| AACACAGGAGCATCAACACT+AGG | 0.653659 | 5_2:-64172452 | None:intergenic |
| AACCTTGTCCTGCTGCAGCC+GGG | 0.469596 | 5_2:-64173227 | None:intergenic |
| AACCTTGTCCTGCTGCTGCC+GGG | 0.402092 | 5_2:-64172280 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATTCTCAACATGATTCAT+AGG | - | chr5_2:64171677-64171696 | None:intergenic | 30.0% | |
| CTCTTATGCTTGCAATAATA+TGG | - | chr5_2:64171892-64171911 | None:intergenic | 30.0% | |
| CTGTTAATTATCCAAATGGA+AGG | + | chr5_2:64172375-64172394 | Msa0781610:CDS | 30.0% | |
| ACATTCTCAACATGATTCAT+AGG | - | chr5_2:64171677-64171696 | None:intergenic | 30.0% | |
| TGGAGTCGACAAATTTATTT+AGG | + | chr5_2:64172826-64172845 | Msa0781610:intron | 30.0% | |
| TTTAATCACAGATCCATACA+CGG | - | chr5_2:64172947-64172966 | None:intergenic | 30.0% | |
| TTACTTTGATACCATTGAAG+TGG | + | chr5_2:64173553-64173572 | Msa0781610:intron | 30.0% | |
| AGAAGGAGAAAAAGAGAAAA+AGG | + | chr5_2:64174530-64174549 | Msa0781610:CDS | 30.0% | |
| TTTAATCACGGATCCATACA+CGG | - | chr5_2:64172099-64172118 | None:intergenic | 35.0% | |
| AAACAGAAATCAAACGGTTC+TGG | + | chr5_2:64172116-64172135 | Msa0781610:intron | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 64171554 | 64174942 | 64171554 | ID=Msa0781610;Name=Msa0781610 |
| chr5_2 | mRNA | 64171554 | 64174942 | 64171554 | ID=Msa0781610-mRNA-1;Parent=Msa0781610;Name=Msa0781610-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|5|0|632 |
| chr5_2 | exon | 64171554 | 64171745 | 64171554 | ID=Msa0781610-mRNA-1:exon:19049;Parent=Msa0781610-mRNA-1 |
| chr5_2 | exon | 64172122 | 64172598 | 64172122 | ID=Msa0781610-mRNA-1:exon:19050;Parent=Msa0781610-mRNA-1 |
| chr5_2 | exon | 64172970 | 64173363 | 64172970 | ID=Msa0781610-mRNA-1:exon:19051;Parent=Msa0781610-mRNA-1 |
| chr5_2 | exon | 64173890 | 64174169 | 64173890 | ID=Msa0781610-mRNA-1:exon:19052;Parent=Msa0781610-mRNA-1 |
| chr5_2 | exon | 64174387 | 64174942 | 64174387 | ID=Msa0781610-mRNA-1:exon:19053;Parent=Msa0781610-mRNA-1 |
| chr5_2 | CDS | 64171554 | 64171745 | 64171554 | ID=Msa0781610-mRNA-1:cds;Parent=Msa0781610-mRNA-1 |
| chr5_2 | CDS | 64172122 | 64172598 | 64172122 | ID=Msa0781610-mRNA-1:cds;Parent=Msa0781610-mRNA-1 |
| chr5_2 | CDS | 64172970 | 64173363 | 64172970 | ID=Msa0781610-mRNA-1:cds;Parent=Msa0781610-mRNA-1 |
| chr5_2 | CDS | 64173890 | 64174169 | 64173890 | ID=Msa0781610-mRNA-1:cds;Parent=Msa0781610-mRNA-1 |
| chr5_2 | CDS | 64174387 | 64174942 | 64174387 | ID=Msa0781610-mRNA-1:cds;Parent=Msa0781610-mRNA-1 |
| Gene Sequence |
>Msa0781610 ATGGTGAGGATCAGAGGAGGAAAGCAGAGGAAAAGTGTTGATGAGAAAAGTGTTGATGTTCCTGTGTTGGTTGTTGGAACCAAGTCTTCGCCAGCTCTTTCTCATGCTCAAACTCCAAAACCTATGAATCATGTTGAGAATGTTTCTGTGCCTAGTGTTGGTCAGGTTGCTCCTAGAGCTCCTTCTGTTCAGAAATCAAACGGTTCTGGAAACTTCAATCCGAAGAATAAGATGCCATCACCGCGCTATCGAAAGCCAGGTCCCAAGCGCAACAGTAACACAAATGGCATGCCTCACTTTCCTGTGGTGACGATGCCTTATCCTAATGGCGAGAATCCTAAGCCTGTATCCCCGGCAGCAGCAGGACAAGGTTTTACTCCACCTGCTCATGCCAATGATGCCAAACATGTTCAGCCTCCGGTGCAAGGAGATCCGAATGCTTATGCTGTTAATTATCCAAATGGAAGGCCAAATACCCAAGAACAAGGTGACCATGTGAATCATGGTCGGCATCATCAAAGACCTAGTGTTGATGCTCCTGTGTTGGTTGTTGGAACCAAGTCTTCGCCGGCTCTTTCTGATGCTCAAACTCCAAAACCTATGAATCATGTTGAGAATGTTTCTGTGCCTAGTGTTGGTCAGGTTGCTCCTATAGCTCCTTCTGTTCAGAAGAAATCAAACGGTTCTGGAAATTTCAATCGGATGAATAAGCTGCCATCACCATGCTATCGAAAGCCAGGCCCCAAGCGCAACAACACAAATGGCACGCTTCACTTTCCTGTGGCGACGAGGCCTTATCATCAGCAGCCGCCTGTTGCTCCGAATTTTCGTCCTAGGGTACCTCCACCTCACATTGCTGTTCCTGCATATGCTTTCCCACCTGGTTCAGGACCTTATCCTAATGGTGAGAATCCTAAGCCTGTATCCCCGGCTGCAGCAGGACAAGGTTTTACTCCACCTGCTCATGCCATTGATGCCAAACATGTTCAGCCTCCGGTGCAAGGAGATCCGAATGCTTATGCTATTGATTATCCAAATGGAAGGCCAAATATCCAAAAACAAGAAAGCATGGAAGAAAGAAAAAGTATACAATCATCCCTCCTTCAAATTGATTTAAATGAGCCGGCTTCAGTCCAAGATCCCTCTGAGCATAGCATGGAACAACAACAGGAGAAAACTGGCACCGATATCATTGAGTCGGGTGGTGCCATTGCAATTCTAAACTCTGATGATAATAACGTCACAACTCTAAACTCTGACGAAAATAACTTAATTCAAGCTGAGTCAGTACCAGAAGAAAGTCATGCCCCAAGGAAAGGGCATTGGACAGAGGATGAACACAAGTTGTTTTTGAAGGGGATGAAAAAACATGGAAAGGGACGCTGGAAAAATATTTCAAAAGAATTTGTGGTGACAAAAACTCCAACTCAAATTGCTAGCCATGCCCAAAAATACTTTATCCACCAAAATGTGAAAGATATGGAGAAGAAGGAGAAAAAGAGAAAAAGGATTCACGACACAACATTAAACAAGAATGACACATTGGTCACTGTCGCTGTTGATCAAGAAGATGAGATTCCTTCAGTTGAGCTACAAAGTGTGATTCCTCCTCAAGGAATGCAACAGACACAAACACAACAGAATGAGATTTCTCCTATGTTGTGCTTGCTGCCAATCGGTTCTACAATTCCTTCGGTTCAGCAACAAAACGAGCTTACTCCTTTAGTCACTGCTCCTATGGAACAACATGAGACTCCTCCTCAAGACACTGCCATCTTAAACAACAAGGACAATGACAACTTGCAAACCCAGTTGGTGTTGCTTTATCCAATCGGTTCTACACTTCCTGATATGAACAAACTTGAGAAGATGCGCAACTTGTTGGCAAAAGAACTTTGA |
| Protein sequence |
>Msa0781610 MVRIRGGKQRKSVDEKSVDVPVLVVGTKSSPALSHAQTPKPMNHVENVSVPSVGQVAPRAPSVQKSNGSGNFNPKNKMPSPRYRKPGPKRNSNTNGMPHFPVVTMPYPNGENPKPVSPAAAGQGFTPPAHANDAKHVQPPVQGDPNAYAVNYPNGRPNTQEQGDHVNHGRHHQRPSVDAPVLVVGTKSSPALSDAQTPKPMNHVENVSVPSVGQVAPIAPSVQKKSNGSGNFNRMNKLPSPCYRKPGPKRNNTNGTLHFPVATRPYHQQPPVAPNFRPRVPPPHIAVPAYAFPPGSGPYPNGENPKPVSPAAAGQGFTPPAHAIDAKHVQPPVQGDPNAYAIDYPNGRPNIQKQESMEERKSIQSSLLQIDLNEPASVQDPSEHSMEQQQEKTGTDIIESGGAIAILNSDDNNVTTLNSDENNLIQAESVPEESHAPRKGHWTEDEHKLFLKGMKKHGKGRWKNISKEFVVTKTPTQIASHAQKYFIHQNVKDMEKKEKKRKRIHDTTLNKNDTLVTVAVDQEDEIPSVELQSVIPPQGMQQTQTQQNEISPMLCLLPIGSTIPSVQQQNELTPLVTAPMEQHETPPQDTAILNNKDNDNLQTQLVLLYPIGSTLPDMNKLEKMRNLLAKEL* |
