Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0784820 | XP_003616581.1 | 98.378 | 185 | 1 | 2 | 1 | 184 | 1 | 184 | 5.72e-131 | 375 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0784820 | sp|Q8H1D2|WOX5_ARATH | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 4.05e-60 | 188 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0784820 | G7KBY3 | 98.378 | 185 | 1 | 2 | 1 | 184 | 1 | 184 | 2.73e-131 | 375 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0784820 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0784820 | MtrunA17_Chr5g0436661 | 98.378 | 185 | 1 | 2 | 1 | 184 | 1 | 184 | 5.26e-135 | 375 |
| Msa0784820 | MtrunA17_Chr7g0238431 | 69.841 | 63 | 19 | 0 | 33 | 95 | 8 | 70 | 8.10e-28 | 103 |
| Msa0784820 | MtrunA17_Chr8g0392991 | 71.429 | 63 | 18 | 0 | 33 | 95 | 96 | 158 | 1.80e-26 | 103 |
| Msa0784820 | MtrunA17_Chr4g0043891 | 61.765 | 68 | 26 | 0 | 33 | 100 | 8 | 75 | 6.89e-26 | 99.8 |
| Msa0784820 | MtrunA17_Chr1g0153171 | 65.152 | 66 | 23 | 0 | 33 | 98 | 90 | 155 | 7.31e-26 | 99.4 |
| Msa0784820 | MtrunA17_Chr5g0405351 | 59.459 | 74 | 26 | 2 | 33 | 105 | 30 | 100 | 1.30e-24 | 97.4 |
| Msa0784820 | MtrunA17_Chr4g0031341 | 57.576 | 66 | 28 | 0 | 33 | 98 | 25 | 90 | 1.01e-23 | 94.4 |
| Msa0784820 | MtrunA17_Chr8g0385441 | 51.163 | 86 | 40 | 1 | 15 | 98 | 62 | 147 | 3.12e-20 | 82.8 |
| Msa0784820 | MtrunA17_Chr3g0125111 | 67.273 | 55 | 18 | 0 | 44 | 98 | 91 | 145 | 7.91e-20 | 81.6 |
| Msa0784820 | MtrunA17_Chr7g0223471 | 45.161 | 62 | 34 | 0 | 33 | 94 | 47 | 108 | 1.55e-15 | 73.6 |
| Msa0784820 | MtrunA17_Chr2g0283141 | 45.312 | 64 | 35 | 0 | 33 | 96 | 55 | 118 | 2.01e-15 | 73.2 |
| Msa0784820 | MtrunA17_Chr1g0212511 | 43.333 | 60 | 34 | 0 | 33 | 92 | 85 | 144 | 7.89e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0784820 | AT3G11260.1 | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 4.12e-61 | 188 |
| Msa0784820 | AT5G05770.1 | 65.487 | 113 | 31 | 3 | 4 | 110 | 2 | 112 | 1.05e-40 | 134 |
| Msa0784820 | AT3G18010.1 | 71.429 | 63 | 18 | 0 | 33 | 95 | 76 | 138 | 7.69e-27 | 104 |
| Msa0784820 | AT2G28610.1 | 65.079 | 63 | 22 | 0 | 33 | 95 | 8 | 70 | 6.12e-26 | 100 |
| Msa0784820 | AT5G59340.1 | 57.333 | 75 | 27 | 1 | 21 | 95 | 7 | 76 | 2.84e-25 | 98.6 |
| Msa0784820 | AT1G46480.2 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 1.18e-24 | 95.5 |
| Msa0784820 | AT1G46480.1 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 6.54e-24 | 94.7 |
| Msa0784820 | AT2G17950.1 | 66.667 | 63 | 20 | 1 | 33 | 94 | 38 | 100 | 9.70e-24 | 95.1 |
| Msa0784820 | AT2G01500.1 | 67.273 | 55 | 18 | 0 | 33 | 87 | 61 | 115 | 1.16e-21 | 89.4 |
| Msa0784820 | AT2G33880.1 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 5.88e-15 | 72.0 |
| Msa0784820 | AT2G33880.2 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 7.03e-15 | 72.0 |
| Msa0784820 | AT5G45980.1 | 46.774 | 62 | 33 | 0 | 33 | 94 | 55 | 116 | 1.79e-14 | 70.5 |
| Msa0784820 | AT4G35550.1 | 42.623 | 61 | 35 | 0 | 33 | 93 | 99 | 159 | 5.73e-12 | 63.2 |
| Msa0784820 | AT1G20700.2 | 35.556 | 90 | 55 | 2 | 19 | 106 | 20 | 108 | 6.26e-12 | 60.8 |
| Msa0784820 | AT1G20700.1 | 35.556 | 90 | 55 | 2 | 19 | 106 | 82 | 170 | 1.24e-11 | 61.2 |
| Msa0784820 | AT1G20700.3 | 38.667 | 75 | 45 | 1 | 19 | 93 | 82 | 155 | 1.25e-11 | 61.2 |
Find 25 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGCAAGAATGTGTTCTAT+TGG | 0.384217 | 5_2:-69541167 | Msa0784820:CDS |
| GATTCACCAAATGAGTTCAA+TGG | 0.392997 | 5_2:+69540712 | None:intergenic |
| AGTTTAACTTGTTCTGTTGT+GGG | 0.394220 | 5_2:+69541282 | None:intergenic |
| AGAGTCTCAATCACTCTATC+AGG | 0.412077 | 5_2:+69540745 | None:intergenic |
| ATGTACAATGGGAGAACAAA+TGG | 0.428304 | 5_2:-69540626 | Msa0784820:CDS |
| AACACCCACCATTGGACTTA+CGG | 0.439714 | 5_2:-69540603 | Msa0784820:CDS |
| GGTTTCAGAATCACAAAGCT+AGG | 0.453724 | 5_2:-69541146 | Msa0784820:CDS |
| AAGTTTAACTTGTTCTGTTG+TGG | 0.462988 | 5_2:+69541281 | None:intergenic |
| TTCTGAATTTGATCAGTGCT+TGG | 0.470346 | 5_2:+69541225 | None:intergenic |
| AGTGGAAGCAGCAGCAGCAC+TGG | 0.493876 | 5_2:-69541327 | Msa0784820:CDS |
| AATATGGAAGAGAGCATGTC+AGG | 0.497698 | 5_2:-69541390 | Msa0784820:CDS |
| TTTGCATACACATGTACAAT+GGG | 0.499345 | 5_2:-69540637 | Msa0784820:CDS |
| AGTAGTTTCCATGGTAAAAG+TGG | 0.503916 | 5_2:-69541345 | Msa0784820:CDS |
| ACAAATGGAACACCCACCAT+TGG | 0.543359 | 5_2:-69540611 | Msa0784820:CDS |
| TCATCTGGAAGTAGTTTCCA+TGG | 0.556114 | 5_2:-69541354 | Msa0784820:CDS |
| GTTTCAGAATCACAAAGCTA+GGG | 0.556495 | 5_2:-69541145 | Msa0784820:CDS |
| GTATGCATGCAAATGAATGC+AGG | 0.569996 | 5_2:-69540672 | Msa0784820:CDS |
| AGCAGCACTGGCACAAAGTG+TGG | 0.575447 | 5_2:-69541315 | Msa0784820:CDS |
| CTCAACCGTAAGTCCAATGG+TGG | 0.576156 | 5_2:+69540598 | None:intergenic |
| GTGAATCAGAATCAGAGAAG+TGG | 0.590084 | 5_2:-69540696 | Msa0784820:CDS |
| CTGGCACAAAGTGTGGTCGT+TGG | 0.591246 | 5_2:-69541308 | Msa0784820:CDS |
| AAACTCAACCGTAAGTCCAA+TGG | 0.615710 | 5_2:+69540595 | None:intergenic |
| GTTTGCATACACATGTACAA+TGG | 0.618786 | 5_2:-69540638 | Msa0784820:CDS |
| TATGCATGCAAATGAATGCA+GGG | 0.627141 | 5_2:-69540671 | Msa0784820:CDS |
| TCAACCGTAAGTCCAATGGT+GGG | 0.711399 | 5_2:+69540599 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTATTTTTCTAACGAGA+TGG | + | chr5_2:69541065-69541084 | None:intergenic | 20.0% |
| !!! | CTTACTAAATTTTTTGCTTT+AGG | - | chr5_2:69541110-69541129 | Msa0784820:CDS | 20.0% |
| !!! | AAATTATTTTTCTAACGAGA+TGG | + | chr5_2:69541065-69541084 | None:intergenic | 20.0% |
| !!! | CTTACTAAATTTTTTGCTTT+AGG | - | chr5_2:69541110-69541129 | Msa0784820:CDS | 20.0% |
| !! | AGTTTAACTTGTTCTGTTGT+GGG | + | chr5_2:69540696-69540715 | None:intergenic | 30.0% |
| !! | AAGTTTAACTTGTTCTGTTG+TGG | + | chr5_2:69540697-69540716 | None:intergenic | 30.0% |
| ! | TCCAATCAACTCAGTTTTTA+TGG | - | chr5_2:69540777-69540796 | Msa0784820:intron | 30.0% |
| !! | ACCATAAAAACTGAGTTGAT+TGG | + | chr5_2:69540781-69540800 | None:intergenic | 30.0% |
| AATGAATAAATGAGTTGCGT+CGG | + | chr5_2:69540971-69540990 | None:intergenic | 30.0% | |
| AGTATCAGATCAAAGTTTCA+AGG | + | chr5_2:69541011-69541030 | None:intergenic | 30.0% | |
| ! | TTCTTTTCCTCATATATTGC+AGG | - | chr5_2:69541185-69541204 | Msa0784820:CDS | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | - | chr5_2:69541186-69541205 | Msa0784820:CDS | 30.0% |
| ! | CTTTTTCCATTGAACTCATT+TGG | - | chr5_2:69541257-69541276 | Msa0784820:CDS | 30.0% |
| TTTGCATACACATGTACAAT+GGG | - | chr5_2:69541338-69541357 | Msa0784820:CDS | 30.0% | |
| !! | AGTTTAACTTGTTCTGTTGT+GGG | + | chr5_2:69540696-69540715 | None:intergenic | 30.0% |
| !! | AAGTTTAACTTGTTCTGTTG+TGG | + | chr5_2:69540697-69540716 | None:intergenic | 30.0% |
| ! | TCCAATCAACTCAGTTTTTA+TGG | - | chr5_2:69540777-69540796 | Msa0784820:intron | 30.0% |
| !! | ACCATAAAAACTGAGTTGAT+TGG | + | chr5_2:69540781-69540800 | None:intergenic | 30.0% |
| AATGAATAAATGAGTTGCGT+CGG | + | chr5_2:69540971-69540990 | None:intergenic | 30.0% | |
| AGTATCAGATCAAAGTTTCA+AGG | + | chr5_2:69541011-69541030 | None:intergenic | 30.0% | |
| ! | TTCTTTTCCTCATATATTGC+AGG | - | chr5_2:69541185-69541204 | Msa0784820:CDS | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | - | chr5_2:69541186-69541205 | Msa0784820:CDS | 30.0% |
| ! | CTTTTTCCATTGAACTCATT+TGG | - | chr5_2:69541257-69541276 | Msa0784820:CDS | 30.0% |
| TTTGCATACACATGTACAAT+GGG | - | chr5_2:69541338-69541357 | Msa0784820:CDS | 30.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | - | chr5_2:69540630-69540649 | Msa0784820:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | + | chr5_2:69540753-69540772 | None:intergenic | 35.0% | |
| AGAGCAAGAATGTGTTCTAT+TGG | - | chr5_2:69540808-69540827 | Msa0784820:intron | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | - | chr5_2:69540830-69540849 | Msa0784820:intron | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | - | chr5_2:69540872-69540891 | Msa0784820:intron | 35.0% |
| TACATGTTACATCATCGTCA+GGG | + | chr5_2:69541157-69541176 | None:intergenic | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | + | chr5_2:69541158-69541177 | None:intergenic | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | + | chr5_2:69541266-69541285 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | - | chr5_2:69541304-69541323 | Msa0784820:CDS | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | - | chr5_2:69541337-69541356 | Msa0784820:CDS | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | - | chr5_2:69541349-69541368 | Msa0784820:CDS | 35.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | - | chr5_2:69540630-69540649 | Msa0784820:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | + | chr5_2:69540753-69540772 | None:intergenic | 35.0% | |
| AGAGCAAGAATGTGTTCTAT+TGG | - | chr5_2:69540808-69540827 | Msa0784820:intron | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | - | chr5_2:69540830-69540849 | Msa0784820:intron | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | - | chr5_2:69540872-69540891 | Msa0784820:intron | 35.0% |
| TACATGTTACATCATCGTCA+GGG | + | chr5_2:69541157-69541176 | None:intergenic | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | + | chr5_2:69541158-69541177 | None:intergenic | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | + | chr5_2:69541266-69541285 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | - | chr5_2:69541304-69541323 | Msa0784820:CDS | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | - | chr5_2:69541337-69541356 | Msa0784820:CDS | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | - | chr5_2:69541349-69541368 | Msa0784820:CDS | 35.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | - | chr5_2:69540606-69540625 | Msa0784820:CDS | 40.0% |
| TCATCTGGAAGTAGTTTCCA+TGG | - | chr5_2:69540621-69540640 | Msa0784820:CDS | 40.0% | |
| ! | TGACTGAACTTTTCAGAGCA+GGG | - | chr5_2:69540718-69540737 | Msa0784820:CDS | 40.0% |
| GGTTTCAGAATCACAAAGCT+AGG | - | chr5_2:69540829-69540848 | Msa0784820:intron | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | - | chr5_2:69540851-69540870 | Msa0784820:intron | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | - | chr5_2:69541126-69541145 | Msa0784820:CDS | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | + | chr5_2:69541195-69541214 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | + | chr5_2:69541233-69541252 | None:intergenic | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | - | chr5_2:69541279-69541298 | Msa0784820:CDS | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | - | chr5_2:69541303-69541322 | Msa0784820:CDS | 40.0% | |
| AAACTCAACCGTAAGTCCAA+TGG | + | chr5_2:69541383-69541402 | None:intergenic | 40.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | - | chr5_2:69540606-69540625 | Msa0784820:CDS | 40.0% |
| TCATCTGGAAGTAGTTTCCA+TGG | - | chr5_2:69540621-69540640 | Msa0784820:CDS | 40.0% | |
| ! | TGACTGAACTTTTCAGAGCA+GGG | - | chr5_2:69540718-69540737 | Msa0784820:CDS | 40.0% |
| GGTTTCAGAATCACAAAGCT+AGG | - | chr5_2:69540829-69540848 | Msa0784820:intron | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | - | chr5_2:69540851-69540870 | Msa0784820:intron | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | - | chr5_2:69541126-69541145 | Msa0784820:CDS | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | + | chr5_2:69541195-69541214 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | + | chr5_2:69541233-69541252 | None:intergenic | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | - | chr5_2:69541279-69541298 | Msa0784820:CDS | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | - | chr5_2:69541303-69541322 | Msa0784820:CDS | 40.0% | |
| AAACTCAACCGTAAGTCCAA+TGG | + | chr5_2:69541383-69541402 | None:intergenic | 40.0% | |
| !! | TGCTGCTTCCACTTTTACCA+TGG | + | chr5_2:69540641-69540660 | None:intergenic | 45.0% |
| ! | CTGACTGAACTTTTCAGAGC+AGG | - | chr5_2:69540717-69540736 | Msa0784820:CDS | 45.0% |
| ACAAATGGAACACCCACCAT+TGG | - | chr5_2:69541364-69541383 | Msa0784820:CDS | 45.0% | |
| AACACCCACCATTGGACTTA+CGG | - | chr5_2:69541372-69541391 | Msa0784820:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | + | chr5_2:69541379-69541398 | None:intergenic | 45.0% | |
| !! | TGCTGCTTCCACTTTTACCA+TGG | + | chr5_2:69540641-69540660 | None:intergenic | 45.0% |
| ! | CTGACTGAACTTTTCAGAGC+AGG | - | chr5_2:69540717-69540736 | Msa0784820:CDS | 45.0% |
| ACAAATGGAACACCCACCAT+TGG | - | chr5_2:69541364-69541383 | Msa0784820:CDS | 45.0% | |
| AACACCCACCATTGGACTTA+CGG | - | chr5_2:69541372-69541391 | Msa0784820:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | + | chr5_2:69541379-69541398 | None:intergenic | 45.0% | |
| !!! | GTTAAAAAAATTATTTTAAA+AGG | - | chr5_2:69541086-69541105 | Msa0784820:CDS | 5.0% |
| !!! | GTTAAAAAAATTATTTTAAA+AGG | - | chr5_2:69541086-69541105 | Msa0784820:CDS | 5.0% |
| GGCCTCTTAATGCATTGGAC+CGG | - | chr5_2:69541131-69541150 | Msa0784820:CDS | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | + | chr5_2:69541153-69541172 | None:intergenic | 50.0% | |
| CTCAACCGTAAGTCCAATGG+TGG | + | chr5_2:69541380-69541399 | None:intergenic | 50.0% | |
| GGCCTCTTAATGCATTGGAC+CGG | - | chr5_2:69541131-69541150 | Msa0784820:CDS | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | + | chr5_2:69541153-69541172 | None:intergenic | 50.0% | |
| CTCAACCGTAAGTCCAATGG+TGG | + | chr5_2:69541380-69541399 | None:intergenic | 50.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | - | chr5_2:69540660-69540679 | Msa0784820:CDS | 55.0% |
| CTGGCACAAAGTGTGGTCGT+TGG | - | chr5_2:69540667-69540686 | Msa0784820:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | + | chr5_2:69541136-69541155 | None:intergenic | 55.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | - | chr5_2:69540660-69540679 | Msa0784820:CDS | 55.0% |
| CTGGCACAAAGTGTGGTCGT+TGG | - | chr5_2:69540667-69540686 | Msa0784820:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | + | chr5_2:69541136-69541155 | None:intergenic | 55.0% | |
| AGTGGAAGCAGCAGCAGCAC+TGG | - | chr5_2:69540648-69540667 | Msa0784820:CDS | 60.0% | |
| AGTGGAAGCAGCAGCAGCAC+TGG | - | chr5_2:69540648-69540667 | Msa0784820:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 69540588 | 69541409 | 69540588 | ID=Msa0784820;Name=Msa0784820 |
| chr5_2 | mRNA | 69540588 | 69541409 | 69540588 | ID=Msa0784820-mRNA-1;Parent=Msa0784820;Name=Msa0784820-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|184 |
| chr5_2 | exon | 69541058 | 69541409 | 69541058 | ID=Msa0784820-mRNA-1:exon:20939;Parent=Msa0784820-mRNA-1 |
| chr5_2 | exon | 69540588 | 69540790 | 69540588 | ID=Msa0784820-mRNA-1:exon:20938;Parent=Msa0784820-mRNA-1 |
| chr5_2 | CDS | 69541058 | 69541409 | 69541058 | ID=Msa0784820-mRNA-1:cds;Parent=Msa0784820-mRNA-1 |
| chr5_2 | CDS | 69540588 | 69540790 | 69540588 | ID=Msa0784820-mRNA-1:cds;Parent=Msa0784820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
