Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0785000 | A0A072TE13 | 79.464 | 112 | 22 | 1 | 1 | 111 | 1 | 112 | 3.08e-54 | 180 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0785000 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0785000 | MtrunA17_Chr1g0172971 | 75.893 | 112 | 26 | 1 | 1 | 111 | 1 | 112 | 1.18e-52 | 173 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGATTCTTTATCATTAT+AGG | 0.134002 | 5_2:+69789827 | None:intergenic |
| TTTGGTCTTTACATCTATTA+AGG | 0.222289 | 5_2:-69789799 | Msa0785000:CDS |
| TCACATATTGGCCTGTTCTT+AGG | 0.241881 | 5_2:+69789615 | None:intergenic |
| TTTGATTATGCATATAATAC+TGG | 0.295362 | 5_2:-69789759 | Msa0785000:CDS |
| TAAAGAATCATGGAAGTCTT+TGG | 0.386457 | 5_2:-69789817 | Msa0785000:CDS |
| ACAAGTGGACTGTCACATAT+TGG | 0.405281 | 5_2:+69789603 | None:intergenic |
| CGTTATGTTTGTAACAAAGA+AGG | 0.419017 | 5_2:-69789663 | Msa0785000:CDS |
| ATCTGAGAGTGGACAATTGA+CGG | 0.452529 | 5_2:-69789892 | Msa0785000:CDS |
| CAATTCAAACAATAAGAAAT+CGG | 0.472441 | 5_2:-69789703 | Msa0785000:CDS |
| GTTTGCTTAATCCTAAGAAC+AGG | 0.477630 | 5_2:-69789626 | Msa0785000:CDS |
| AAACAATAAGAAATCGGATA+AGG | 0.493802 | 5_2:-69789697 | Msa0785000:CDS |
| CCTATAATGATAAAGAATCA+TGG | 0.517504 | 5_2:-69789827 | Msa0785000:CDS |
| TAACAAAGAAGGATTCAAGA+AGG | 0.538422 | 5_2:-69789652 | Msa0785000:CDS |
| TAGTTCTCATACTCAACAAG+TGG | 0.556390 | 5_2:+69789588 | None:intergenic |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 5_2:-69789868 | Msa0785000:CDS |
| AACAAAGAAGGATTCAAGAA+GGG | 0.609727 | 5_2:-69789651 | Msa0785000:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 5_2:-69789903 | Msa0785000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGATTATGCATATAATAC+TGG | - | chr5_2:69789740-69789759 | Msa0785000:CDS | 20.0% |
| !! | CAATTCAAACAATAAGAAAT+CGG | - | chr5_2:69789796-69789815 | Msa0785000:CDS | 20.0% |
| ! | TGAAAATTCAGTTATTGATG+CGG | - | chr5_2:69789631-69789650 | Msa0785000:CDS | 25.0% |
| ! | CCATGATTCTTTATCATTAT+AGG | + | chr5_2:69789675-69789694 | None:intergenic | 25.0% |
| ! | CCTATAATGATAAAGAATCA+TGG | - | chr5_2:69789672-69789691 | Msa0785000:CDS | 25.0% |
| !! | TTTGGTCTTTACATCTATTA+AGG | - | chr5_2:69789700-69789719 | Msa0785000:CDS | 25.0% |
| !! | ATAATACTGGATTTTCTGTT+AGG | - | chr5_2:69789753-69789772 | Msa0785000:CDS | 25.0% |
| ! | AAACAATAAGAAATCGGATA+AGG | - | chr5_2:69789802-69789821 | Msa0785000:CDS | 25.0% |
| TAAAGAATCATGGAAGTCTT+TGG | - | chr5_2:69789682-69789701 | Msa0785000:CDS | 30.0% | |
| CGTTATGTTTGTAACAAAGA+AGG | - | chr5_2:69789836-69789855 | Msa0785000:CDS | 30.0% | |
| TAACAAAGAAGGATTCAAGA+AGG | - | chr5_2:69789847-69789866 | Msa0785000:CDS | 30.0% | |
| AACAAAGAAGGATTCAAGAA+GGG | - | chr5_2:69789848-69789867 | Msa0785000:CDS | 30.0% | |
| ATATCAACTTCATCTGAGAG+TGG | - | chr5_2:69789596-69789615 | Msa0785000:CDS | 35.0% | |
| GTTTGCTTAATCCTAAGAAC+AGG | - | chr5_2:69789873-69789892 | Msa0785000:CDS | 35.0% | |
| TAGTTCTCATACTCAACAAG+TGG | + | chr5_2:69789914-69789933 | None:intergenic | 35.0% | |
| ATCTGAGAGTGGACAATTGA+CGG | - | chr5_2:69789607-69789626 | Msa0785000:CDS | 40.0% | |
| ! | TCACATATTGGCCTGTTCTT+AGG | + | chr5_2:69789887-69789906 | None:intergenic | 40.0% |
| !! | ACAAGTGGACTGTCACATAT+TGG | + | chr5_2:69789899-69789918 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 69789587 | 69789934 | 69789587 | ID=Msa0785000;Name=Msa0785000 |
| chr5_2 | mRNA | 69789587 | 69789934 | 69789587 | ID=Msa0785000-mRNA-1;Parent=Msa0785000;Name=Msa0785000-mRNA-1;_AED=0.15;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|115 |
| chr5_2 | exon | 69789587 | 69789934 | 69789587 | ID=Msa0785000-mRNA-1:exon:21045;Parent=Msa0785000-mRNA-1 |
| chr5_2 | CDS | 69789587 | 69789934 | 69789587 | ID=Msa0785000-mRNA-1:cds;Parent=Msa0785000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
