Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0785020 | sp|Q8S9H7|DIV_ANTMA | 58.411 | 214 | 76 | 5 | 3 | 205 | 1 | 212 | 7.35e-78 | 241 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0785020 | G7KBX0 | 98.893 | 271 | 2 | 1 | 1 | 270 | 1 | 271 | 0.0 | 560 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0785020 | TF | MYB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0785020 | MtrunA17_Chr5g0436501 | 98.893 | 271 | 2 | 1 | 1 | 270 | 1 | 271 | 0.0 | 560 |
| Msa0785020 | MtrunA17_Chr7g0241761 | 62.150 | 214 | 71 | 3 | 3 | 208 | 11 | 222 | 3.95e-88 | 264 |
| Msa0785020 | MtrunA17_Chr5g0429521 | 61.386 | 202 | 71 | 3 | 2 | 198 | 4 | 203 | 7.81e-85 | 256 |
| Msa0785020 | MtrunA17_Chr3g0104071 | 64.796 | 196 | 56 | 4 | 16 | 200 | 27 | 220 | 9.25e-83 | 251 |
| Msa0785020 | MtrunA17_Chr1g0162081 | 53.005 | 183 | 70 | 4 | 25 | 194 | 10 | 189 | 2.58e-55 | 180 |
| Msa0785020 | MtrunA17_Chr1g0210471 | 51.412 | 177 | 72 | 4 | 25 | 194 | 22 | 191 | 1.73e-54 | 176 |
| Msa0785020 | MtrunA17_Chr4g0062741 | 43.350 | 203 | 75 | 5 | 27 | 194 | 8 | 205 | 3.03e-47 | 159 |
| Msa0785020 | MtrunA17_Chr8g0365011 | 44.022 | 184 | 73 | 4 | 25 | 194 | 6 | 173 | 8.23e-38 | 134 |
| Msa0785020 | MtrunA17_Chr6g0488471 | 69.863 | 73 | 20 | 1 | 119 | 191 | 91 | 161 | 7.89e-29 | 110 |
| Msa0785020 | MtrunA17_Chr2g0331031 | 63.333 | 90 | 30 | 2 | 120 | 208 | 104 | 191 | 9.61e-29 | 112 |
| Msa0785020 | MtrunA17_Chr4g0055931 | 64.198 | 81 | 27 | 1 | 120 | 200 | 90 | 168 | 7.04e-28 | 109 |
| Msa0785020 | MtrunA17_Chr8g0389241 | 49.123 | 114 | 55 | 1 | 116 | 229 | 96 | 206 | 1.85e-27 | 107 |
| Msa0785020 | MtrunA17_Chr5g0415191 | 60.674 | 89 | 33 | 1 | 112 | 200 | 89 | 175 | 9.50e-26 | 103 |
| Msa0785020 | MtrunA17_Chr4g0059701 | 71.186 | 59 | 17 | 0 | 113 | 171 | 72 | 130 | 1.07e-24 | 100 |
| Msa0785020 | MtrunA17_Chr5g0409841 | 49.524 | 105 | 49 | 1 | 92 | 192 | 55 | 159 | 8.00e-24 | 96.3 |
| Msa0785020 | MtrunA17_Chr8g0364741 | 40.984 | 122 | 66 | 2 | 25 | 142 | 8 | 127 | 1.15e-23 | 93.2 |
| Msa0785020 | MtrunA17_Chr5g0440741 | 53.933 | 89 | 39 | 2 | 105 | 192 | 81 | 168 | 4.88e-23 | 95.5 |
| Msa0785020 | MtrunA17_Chr3g0135671 | 51.429 | 105 | 44 | 4 | 90 | 191 | 58 | 158 | 8.88e-23 | 93.6 |
| Msa0785020 | MtrunA17_Chr5g0440731 | 58.904 | 73 | 29 | 1 | 121 | 192 | 101 | 173 | 9.00e-23 | 94.7 |
| Msa0785020 | MtrunA17_Chr1g0155181 | 55.882 | 68 | 30 | 0 | 22 | 89 | 2 | 69 | 3.96e-21 | 85.1 |
| Msa0785020 | MtrunA17_Chr3g0092621 | 53.247 | 77 | 34 | 1 | 125 | 201 | 84 | 158 | 5.71e-21 | 87.4 |
| Msa0785020 | MtrunA17_Chr3g0141601 | 53.846 | 65 | 30 | 0 | 25 | 89 | 5 | 69 | 1.25e-19 | 81.3 |
| Msa0785020 | MtrunA17_Chr6g0449111 | 52.941 | 68 | 32 | 0 | 22 | 89 | 5 | 72 | 8.22e-19 | 78.6 |
| Msa0785020 | MtrunA17_Chr5g0409831 | 44.643 | 112 | 52 | 2 | 121 | 231 | 73 | 175 | 1.05e-18 | 82.4 |
| Msa0785020 | MtrunA17_Chr7g0255841 | 52.941 | 68 | 32 | 0 | 30 | 97 | 15 | 82 | 4.07e-18 | 77.4 |
| Msa0785020 | MtrunA17_Chr5g0432921 | 34.706 | 170 | 102 | 6 | 26 | 194 | 20 | 181 | 6.05e-18 | 80.5 |
| Msa0785020 | MtrunA17_Chr3g0113121 | 39.877 | 163 | 84 | 7 | 48 | 202 | 21 | 177 | 7.77e-18 | 79.7 |
| Msa0785020 | MtrunA17_Chr6g0452791 | 57.627 | 59 | 25 | 0 | 125 | 183 | 80 | 138 | 9.80e-18 | 78.6 |
| Msa0785020 | MtrunA17_Chr3g0144631 | 52.381 | 63 | 30 | 0 | 27 | 89 | 16 | 78 | 1.48e-17 | 75.5 |
| Msa0785020 | MtrunA17_Chr8g0364731 | 65.000 | 60 | 21 | 0 | 135 | 194 | 11 | 70 | 3.72e-17 | 77.4 |
| Msa0785020 | MtrunA17_Chr5g0404101 | 45.333 | 75 | 41 | 0 | 15 | 89 | 7 | 81 | 3.99e-17 | 74.7 |
| Msa0785020 | MtrunA17_Chr3g0081631 | 47.945 | 73 | 37 | 1 | 18 | 89 | 1 | 73 | 1.04e-16 | 73.6 |
| Msa0785020 | MtrunA17_Chr5g0432941 | 31.176 | 170 | 107 | 4 | 26 | 194 | 21 | 181 | 3.10e-16 | 75.9 |
| Msa0785020 | MtrunA17_Chr8g0373131 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.24e-15 | 69.3 |
| Msa0785020 | MtrunA17_Chr8g0373111 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.34e-15 | 69.3 |
| Msa0785020 | MtrunA17_Chr8g0373121 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.34e-15 | 69.3 |
| Msa0785020 | MtrunA17_Chr8g0373101 | 50.820 | 61 | 29 | 1 | 27 | 87 | 5 | 64 | 4.53e-14 | 65.9 |
| Msa0785020 | MtrunA17_Chr5g0404301 | 50.820 | 61 | 29 | 1 | 27 | 87 | 4 | 63 | 2.27e-13 | 63.5 |
| Msa0785020 | MtrunA17_Chr1g0192061 | 55.714 | 70 | 29 | 2 | 127 | 195 | 63 | 131 | 2.72e-13 | 66.6 |
| Msa0785020 | MtrunA17_Chr3g0141641 | 38.806 | 67 | 41 | 0 | 27 | 93 | 3 | 69 | 3.64e-13 | 63.9 |
| Msa0785020 | MtrunA17_Chr8g0370121 | 50.000 | 50 | 25 | 0 | 122 | 171 | 97 | 146 | 7.75e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0785020 | AT2G38090.1 | 59.716 | 211 | 69 | 2 | 3 | 199 | 5 | 213 | 4.94e-86 | 259 |
| Msa0785020 | AT3G11280.2 | 66.138 | 189 | 54 | 3 | 27 | 211 | 33 | 215 | 2.95e-81 | 245 |
| Msa0785020 | AT3G11280.1 | 66.138 | 189 | 54 | 3 | 27 | 211 | 33 | 215 | 2.95e-81 | 245 |
| Msa0785020 | AT5G05790.2 | 56.388 | 227 | 88 | 6 | 3 | 221 | 4 | 227 | 5.26e-79 | 240 |
| Msa0785020 | AT5G05790.1 | 56.388 | 227 | 88 | 6 | 3 | 221 | 4 | 227 | 5.26e-79 | 240 |
| Msa0785020 | AT5G58900.1 | 58.019 | 212 | 69 | 5 | 16 | 209 | 15 | 224 | 2.78e-75 | 231 |
| Msa0785020 | AT5G01200.1 | 54.067 | 209 | 73 | 6 | 6 | 196 | 12 | 215 | 8.57e-67 | 209 |
| Msa0785020 | AT5G08520.1 | 51.099 | 182 | 79 | 2 | 27 | 200 | 12 | 191 | 1.10e-52 | 174 |
| Msa0785020 | AT1G49010.1 | 45.050 | 202 | 81 | 4 | 21 | 194 | 2 | 201 | 1.29e-49 | 166 |
| Msa0785020 | AT5G04760.1 | 43.541 | 209 | 89 | 6 | 25 | 230 | 4 | 186 | 1.44e-47 | 158 |
| Msa0785020 | AT5G23650.1 | 42.857 | 210 | 89 | 5 | 20 | 214 | 6 | 199 | 3.14e-44 | 153 |
| Msa0785020 | AT3G10580.2 | 39.320 | 206 | 96 | 8 | 27 | 230 | 8 | 186 | 3.26e-35 | 127 |
| Msa0785020 | AT3G10580.1 | 39.320 | 206 | 96 | 8 | 27 | 230 | 8 | 186 | 7.82e-35 | 127 |
| Msa0785020 | AT4G09450.1 | 42.515 | 167 | 80 | 4 | 26 | 192 | 6 | 156 | 7.11e-32 | 117 |
| Msa0785020 | AT5G47390.1 | 66.250 | 80 | 25 | 1 | 112 | 191 | 83 | 160 | 1.48e-27 | 109 |
| Msa0785020 | AT5G56840.1 | 66.667 | 75 | 24 | 1 | 120 | 194 | 86 | 159 | 2.16e-27 | 106 |
| Msa0785020 | AT5G61620.1 | 52.941 | 102 | 46 | 1 | 91 | 192 | 74 | 173 | 8.15e-27 | 106 |
| Msa0785020 | AT1G19000.2 | 51.327 | 113 | 47 | 3 | 112 | 218 | 89 | 199 | 3.69e-25 | 101 |
| Msa0785020 | AT1G19000.1 | 51.327 | 113 | 47 | 3 | 112 | 218 | 89 | 199 | 3.69e-25 | 101 |
| Msa0785020 | AT1G70000.1 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 7.48e-25 | 100 |
| Msa0785020 | AT1G70000.2 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 7.48e-25 | 100 |
| Msa0785020 | AT3G16350.1 | 55.238 | 105 | 41 | 3 | 116 | 216 | 125 | 227 | 3.68e-24 | 100 |
| Msa0785020 | AT3G10590.1 | 41.799 | 189 | 84 | 12 | 27 | 201 | 7 | 183 | 5.04e-23 | 94.0 |
| Msa0785020 | AT1G74840.2 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 6.05e-22 | 92.0 |
| Msa0785020 | AT1G74840.1 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 8.91e-22 | 92.0 |
| Msa0785020 | AT1G75250.2 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 6.48e-18 | 77.4 |
| Msa0785020 | AT1G75250.1 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 8.83e-18 | 77.8 |
| Msa0785020 | AT4G39250.1 | 48.485 | 66 | 34 | 0 | 27 | 92 | 14 | 79 | 1.51e-17 | 76.3 |
| Msa0785020 | AT2G21650.1 | 47.761 | 67 | 35 | 0 | 27 | 93 | 14 | 80 | 3.98e-16 | 72.8 |
| Msa0785020 | AT2G18328.1 | 54.098 | 61 | 27 | 1 | 29 | 89 | 14 | 73 | 4.62e-14 | 66.2 |
| Msa0785020 | AT1G19510.1 | 51.724 | 58 | 28 | 0 | 32 | 89 | 17 | 74 | 4.05e-13 | 64.3 |
| Msa0785020 | AT3G10595.1 | 29.054 | 148 | 81 | 5 | 24 | 171 | 3 | 126 | 3.60e-12 | 63.9 |
Find 81 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATTGCAGTTTGCTTTA+AGG | 0.090941 | 5_2:+69840985 | None:intergenic |
| CTCAATCCTTTAACAGAATT+TGG | 0.092721 | 5_2:-69840961 | Msa0785020:three_prime_UTR |
| AATAAGTTCTTATCTAAATT+TGG | 0.139369 | 5_2:-69840739 | Msa0785020:three_prime_UTR |
| TCCACTCCAAATTCTGTTAA+AGG | 0.150880 | 5_2:+69840955 | None:intergenic |
| CACATAGTCACATACATAAA+TGG | 0.179244 | 5_2:+69843114 | None:intergenic |
| GTAGTAGTATTTGTGATTTA+AGG | 0.198334 | 5_2:-69842566 | Msa0785020:five_prime_UTR |
| GTCTCCTATGCAGCAGAAAA+TGG | 0.222210 | 5_2:-69841207 | Msa0785020:CDS |
| TATGCTAAGCTGAAAGTTTC+GGG | 0.251091 | 5_2:-69841110 | Msa0785020:CDS |
| ACCAGGAATCGGAACATTTC+CGG | 0.268577 | 5_2:+69842301 | None:intergenic |
| AGAAGATATGAAAATATTAT+AGG | 0.300464 | 5_2:+69842921 | None:intergenic |
| GCCGGAAATGTTCCGATTCC+TGG | 0.317971 | 5_2:-69842302 | Msa0785020:CDS |
| TTATGCTAAGCTGAAAGTTT+CGG | 0.326332 | 5_2:-69841111 | Msa0785020:CDS |
| CTTCTCTACTCCATTCTGTT+TGG | 0.332735 | 5_2:+69842474 | None:intergenic |
| TAGTGATCATGTCTATCTTT+TGG | 0.334038 | 5_2:-69840884 | Msa0785020:three_prime_UTR |
| AGGAGACACATTGACTAATA+AGG | 0.335368 | 5_2:+69841223 | None:intergenic |
| TGATGTGATTAAGCAGTATA+GGG | 0.335951 | 5_2:-69842354 | Msa0785020:CDS |
| ACATAAAATAAGGAGAATAA+AGG | 0.337256 | 5_2:+69842528 | None:intergenic |
| TGTGATTTAAGGTATGGATT+TGG | 0.345223 | 5_2:-69842555 | Msa0785020:exon |
| AGGGTGGTGTATAATATTCT+TGG | 0.349156 | 5_2:-69842841 | Msa0785020:five_prime_UTR |
| AGATTCTGTATGATACTAAA+AGG | 0.365142 | 5_2:-69842733 | Msa0785020:five_prime_UTR |
| AGAAGGCATGTGACTGTTAG+AGG | 0.384719 | 5_2:-69842218 | Msa0785020:CDS |
| TTGATGTGATTAAGCAGTAT+AGG | 0.386148 | 5_2:-69842355 | Msa0785020:CDS |
| AAGATTGCTGAGATGATTCC+TGG | 0.391655 | 5_2:-69842389 | Msa0785020:CDS |
| ATATTCAACCTTTCTTTAAG+AGG | 0.395823 | 5_2:-69842861 | Msa0785020:five_prime_UTR |
| AGTATTTGTGATTTAAGGTA+TGG | 0.403756 | 5_2:-69842561 | Msa0785020:exon |
| AGAGTAGAAGTGAGTTCAAA+AGG | 0.404106 | 5_2:+69843157 | None:intergenic |
| TTTGTATCCAACATAAAATA+AGG | 0.405866 | 5_2:+69842518 | None:intergenic |
| ATAAGGCAAAAGGTTTCTTC+TGG | 0.415949 | 5_2:-69841326 | Msa0785020:CDS |
| GCAATCTTAAGCCATCTATC+TGG | 0.417904 | 5_2:+69842404 | None:intergenic |
| ATCACAAATACTACTACAAA+AGG | 0.439751 | 5_2:+69842572 | None:intergenic |
| TAAAGTATGGCAAAGGCGAC+TGG | 0.445487 | 5_2:-69841421 | Msa0785020:CDS |
| CATAAAATAAGGAGAATAAA+GGG | 0.445828 | 5_2:+69842529 | None:intergenic |
| GATTAAGCAGTATAGGGAAT+TGG | 0.449715 | 5_2:-69842348 | Msa0785020:CDS |
| GCATGACTTGCTACTTGTGT+TGG | 0.455619 | 5_2:+69841362 | None:intergenic |
| ACATAGTCACATACATAAAT+GGG | 0.457280 | 5_2:+69843115 | None:intergenic |
| TGCTCTTGCTATATATGATA+AGG | 0.457525 | 5_2:-69842432 | Msa0785020:CDS |
| TGAGACACAGCATGTCTGAA+TGG | 0.469648 | 5_2:+69840835 | None:intergenic |
| TACACCACCCTCTTAAAGAA+AGG | 0.470360 | 5_2:+69842853 | None:intergenic |
| CACTTTGTCGTTTCTTCATG+AGG | 0.474646 | 5_2:+69843062 | None:intergenic |
| ATAAGGATACACCAGATAGA+TGG | 0.475384 | 5_2:-69842415 | Msa0785020:CDS |
| ATGCACAAAAGTACTACATA+AGG | 0.480834 | 5_2:-69841343 | Msa0785020:CDS |
| AGAGGAAGAAAGGTGTTCCT+TGG | 0.489408 | 5_2:-69842184 | Msa0785020:CDS |
| AAATACTACTACAAAAGGAT+TGG | 0.490445 | 5_2:+69842577 | None:intergenic |
| TGAGTTCAAAAGGAAGAAGA+AGG | 0.490622 | 5_2:+69843167 | None:intergenic |
| CAAGAGCAAGAGGGTGAAAG+AGG | 0.493861 | 5_2:+69842950 | None:intergenic |
| GAGACACAGCATGTCTGAAT+GGG | 0.499137 | 5_2:+69840836 | None:intergenic |
| AAAGTACTACATAAGGCAAA+AGG | 0.510073 | 5_2:-69841336 | Msa0785020:CDS |
| TATTCAACCTTTCTTTAAGA+GGG | 0.510102 | 5_2:-69842860 | Msa0785020:five_prime_UTR |
| GAAGCAACAATAAAAGCAAA+TGG | 0.511111 | 5_2:+69843084 | None:intergenic |
| AATAAGGACTTGTTATCTGA+TGG | 0.512003 | 5_2:+69841239 | None:intergenic |
| GTTGATGACATGAAGCTACT+TGG | 0.515565 | 5_2:-69841025 | Msa0785020:three_prime_UTR |
| GAGGCAAGATAACCAGGAAT+CGG | 0.520347 | 5_2:+69842290 | None:intergenic |
| TGATGGATCACCACAAGGGC+AGG | 0.524353 | 5_2:-69841162 | Msa0785020:CDS |
| TCAGCTTAGCATAAGCCTCA+TGG | 0.535139 | 5_2:+69841120 | None:intergenic |
| TCTCCTATGCAGCAGAAAAT+GGG | 0.535185 | 5_2:-69841206 | Msa0785020:CDS |
| AGTACTAGCCACTACAATGA+TGG | 0.540673 | 5_2:-69841179 | Msa0785020:CDS |
| TCTGATCATGAGAGGAAGAA+AGG | 0.544690 | 5_2:-69842194 | Msa0785020:CDS |
| TCAACCTTTCTTTAAGAGGG+TGG | 0.551667 | 5_2:-69842857 | Msa0785020:five_prime_UTR |
| AAAGAAGAGGCAAGATAACC+AGG | 0.552310 | 5_2:+69842284 | None:intergenic |
| AAGAGAGTTACCAAACAGAA+TGG | 0.556624 | 5_2:-69842484 | Msa0785020:CDS |
| TCCTTTAACAGAATTTGGAG+TGG | 0.565291 | 5_2:-69840956 | Msa0785020:three_prime_UTR |
| AGGCAAAAGGTTTCTTCTGG+AGG | 0.582472 | 5_2:-69841323 | Msa0785020:CDS |
| AAGAGCAAGAGGGTGAAAGA+GGG | 0.582574 | 5_2:+69842951 | None:intergenic |
| ACTATGATGGAAACAGAAGA+AGG | 0.582942 | 5_2:-69842235 | Msa0785020:CDS |
| TCATAGAGATCCTGCCCTTG+TGG | 0.585420 | 5_2:+69841152 | None:intergenic |
| CTTGGACTGAAGAGGAACAT+AGG | 0.585629 | 5_2:-69842166 | Msa0785020:intron |
| CTATGTTCCTCTTCAGTCCA+AGG | 0.586053 | 5_2:+69842167 | None:intergenic |
| CTATGCAGCAGAAAATGGGT+TGG | 0.587490 | 5_2:-69841202 | Msa0785020:CDS |
| AGGTGTTCCTTGGACTGAAG+AGG | 0.592742 | 5_2:-69842174 | Msa0785020:CDS |
| TAGGAACATTCAAGAGCAAG+AGG | 0.598552 | 5_2:+69842940 | None:intergenic |
| GTTGTGATATCATGAATGCT+TGG | 0.600782 | 5_2:+69841284 | None:intergenic |
| CTATGACTGCTCTTTCCATG+AGG | 0.601527 | 5_2:-69841135 | Msa0785020:CDS |
| GCTTCTAGAGACTTCAACAA+AGG | 0.612158 | 5_2:-69841077 | Msa0785020:CDS |
| GATGTGAGTGAAATTGAGGC+CGG | 0.613302 | 5_2:-69842320 | Msa0785020:CDS |
| TACAATGATGGATCACCACA+AGG | 0.622159 | 5_2:-69841167 | Msa0785020:CDS |
| AACTTCAAAGGTGAAAGAAG+AGG | 0.626616 | 5_2:+69842271 | None:intergenic |
| TTAGAGGTTCTGATCATGAG+AGG | 0.633143 | 5_2:-69842202 | Msa0785020:CDS |
| GTGGTGATCCATCATTGTAG+TGG | 0.633599 | 5_2:+69841171 | None:intergenic |
| AGAAGATGTGAGTGAAATTG+AGG | 0.635385 | 5_2:-69842324 | Msa0785020:CDS |
| AGGAACATTCAAGAGCAAGA+GGG | 0.687281 | 5_2:+69842941 | None:intergenic |
| ACAATGATGGATCACCACAA+GGG | 0.720384 | 5_2:-69841166 | Msa0785020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAGATATGAAAATATTAT+AGG | + | chr5_2:69841017-69841036 | None:intergenic | 15.0% |
| !! | AAATAAAAAACCTAAAATCT+AGG | + | chr5_2:69842129-69842148 | None:intergenic | 15.0% |
| !!! | ATAACAAGTAACTTTTAAAA+GGG | + | chr5_2:69842303-69842322 | None:intergenic | 15.0% |
| !!! | AATAACAAGTAACTTTTAAA+AGG | + | chr5_2:69842304-69842323 | None:intergenic | 15.0% |
| !!! | ATAACAAGTAACTTTTAAAA+GGG | + | chr5_2:69842303-69842322 | None:intergenic | 15.0% |
| !!! | AATAACAAGTAACTTTTAAA+AGG | + | chr5_2:69842304-69842323 | None:intergenic | 15.0% |
| !! | AATAAGTTCTTATCTAAATT+TGG | - | chr5_2:69843196-69843215 | Msa0785020:five_prime_UTR | 15.0% |
| !!! | TATCTTTTTTTTCTCTCTAT+TGG | - | chr5_2:69840942-69840961 | Msa0785020:three_prime_UTR | 20.0% |
| !!! | TTCATATCTTCTATCTTTTT+TGG | - | chr5_2:69841025-69841044 | Msa0785020:three_prime_UTR | 20.0% |
| !! | CATAAAATAAGGAGAATAAA+GGG | + | chr5_2:69841409-69841428 | None:intergenic | 20.0% |
| !! | ACATAAAATAAGGAGAATAA+AGG | + | chr5_2:69841410-69841429 | None:intergenic | 20.0% |
| !!! | TTATTCTCCTTATTTTATGT+TGG | - | chr5_2:69841410-69841429 | Msa0785020:CDS | 20.0% |
| !! | TTTGTATCCAACATAAAATA+AGG | + | chr5_2:69841420-69841439 | None:intergenic | 20.0% |
| !!! | ATTTTATGTTGGATACAAAT+TGG | - | chr5_2:69841421-69841440 | Msa0785020:CDS | 20.0% |
| !! | AGTAAAAGAACAAAAAGAAA+AGG | + | chr5_2:69841826-69841845 | None:intergenic | 20.0% |
| !!! | TTTTTCTAGTAGTATACTAT+AGG | + | chr5_2:69841893-69841912 | None:intergenic | 20.0% |
| !!! | TAACAAGTAACTTTTAAAAG+GGG | + | chr5_2:69842302-69842321 | None:intergenic | 20.0% |
| !! | ATTGAACTTGATTATTAGTT+TGG | - | chr5_2:69842321-69842340 | Msa0785020:CDS | 20.0% |
| !!! | TAACAAGTAACTTTTAAAAG+GGG | + | chr5_2:69842302-69842321 | None:intergenic | 20.0% |
| ! | ACATAGTCACATACATAAAT+GGG | + | chr5_2:69840823-69840842 | None:intergenic | 25.0% |
| ! | ATCAAACAAAGGAAAAAAGA+TGG | + | chr5_2:69840911-69840930 | None:intergenic | 25.0% |
| !! | GATTCTTTTCAATCAAACAA+AGG | + | chr5_2:69840922-69840941 | None:intergenic | 25.0% |
| ! | ATATTCAACCTTTCTTTAAG+AGG | - | chr5_2:69841074-69841093 | Msa0785020:CDS | 25.0% |
| !! | TATTCAACCTTTCTTTAAGA+GGG | - | chr5_2:69841075-69841094 | Msa0785020:CDS | 25.0% |
| !!! | TTTGAGACTATTGATTTTGA+TGG | + | chr5_2:69841126-69841145 | None:intergenic | 25.0% |
| ! | AGATTCTGTATGATACTAAA+AGG | - | chr5_2:69841202-69841221 | Msa0785020:CDS | 25.0% |
| !!! | CTATAATTTCCTCTTTTTTG+TGG | - | chr5_2:69841317-69841336 | Msa0785020:CDS | 25.0% |
| ! | CATAAAAAACCACAAAAAAG+AGG | + | chr5_2:69841329-69841348 | None:intergenic | 25.0% |
| ! | AAATACTACTACAAAAGGAT+TGG | + | chr5_2:69841361-69841380 | None:intergenic | 25.0% |
| ! | ATCACAAATACTACTACAAA+AGG | + | chr5_2:69841366-69841385 | None:intergenic | 25.0% |
| ! | GTAGTAGTATTTGTGATTTA+AGG | - | chr5_2:69841369-69841388 | Msa0785020:CDS | 25.0% |
| !! | AGTATTTGTGATTTAAGGTA+TGG | - | chr5_2:69841374-69841393 | Msa0785020:CDS | 25.0% |
| ! | AGAAAAATTGAAGTAAGACA+CGG | - | chr5_2:69841906-69841925 | Msa0785020:intron | 25.0% |
| ! | AGAGACGGAAAAAATAAAAT+CGG | - | chr5_2:69841934-69841953 | Msa0785020:intron | 25.0% |
| ! | TAGCTAATGAATCATATTCA+TGG | - | chr5_2:69842074-69842093 | Msa0785020:intron | 25.0% |
| !! | GAATCATTTTCCTAGATTTT+AGG | - | chr5_2:69842116-69842135 | Msa0785020:intron | 25.0% |
| !!! | ATGTTAAATTGAGTTGGTTT+AGG | - | chr5_2:69842394-69842413 | Msa0785020:CDS | 25.0% |
| CACATAGTCACATACATAAA+TGG | + | chr5_2:69840824-69840843 | None:intergenic | 30.0% | |
| GAAGCAACAATAAAAGCAAA+TGG | + | chr5_2:69840854-69840873 | None:intergenic | 30.0% | |
| ! | TGTGATTTAAGGTATGGATT+TGG | - | chr5_2:69841380-69841399 | Msa0785020:CDS | 30.0% |
| ! | TGCTCTTGCTATATATGATA+AGG | - | chr5_2:69841503-69841522 | Msa0785020:intron | 30.0% |
| TTGATGTGATTAAGCAGTAT+AGG | - | chr5_2:69841580-69841599 | Msa0785020:intron | 30.0% | |
| TGATGTGATTAAGCAGTATA+GGG | - | chr5_2:69841581-69841600 | Msa0785020:intron | 30.0% | |
| !! | TTGTTTTTCGACAACTTCAA+AGG | + | chr5_2:69841679-69841698 | None:intergenic | 30.0% |
| GTCGAAAAACAAAACTATGA+TGG | - | chr5_2:69841687-69841706 | Msa0785020:intron | 30.0% | |
| ! | TTCTTTTACTCCTTTCTCTA+TGG | - | chr5_2:69841836-69841855 | Msa0785020:intron | 30.0% |
| GAAAAAACTTCCATAGAGAA+AGG | + | chr5_2:69841849-69841868 | None:intergenic | 30.0% | |
| !!! | GGAAGTTTTTTCTTAGATTG+TGG | - | chr5_2:69841857-69841876 | Msa0785020:intron | 30.0% |
| !! | GTAACTTTTAAAAGGGGTAA+AGG | + | chr5_2:69842296-69842315 | None:intergenic | 30.0% |
| ATCGACATGTTAAATTGAGT+TGG | - | chr5_2:69842388-69842407 | Msa0785020:CDS | 30.0% | |
| !! | GTAACTTTTAAAAGGGGTAA+AGG | + | chr5_2:69842296-69842315 | None:intergenic | 30.0% |
| ATTTACTCTTTGTGCATGTT+TGG | - | chr5_2:69842456-69842475 | Msa0785020:CDS | 30.0% | |
| !! | ATGCACAAAAGTACTACATA+AGG | - | chr5_2:69842592-69842611 | Msa0785020:five_prime_UTR | 30.0% |
| ! | AAAGTACTACATAAGGCAAA+AGG | - | chr5_2:69842599-69842618 | Msa0785020:five_prime_UTR | 30.0% |
| !! | AATAAGGACTTGTTATCTGA+TGG | + | chr5_2:69842699-69842718 | None:intergenic | 30.0% |
| TTATGCTAAGCTGAAAGTTT+CGG | - | chr5_2:69842824-69842843 | Msa0785020:five_prime_UTR | 30.0% | |
| ! | CATTATTGCAGTTTGCTTTA+AGG | + | chr5_2:69842953-69842972 | None:intergenic | 30.0% |
| CTCAATCCTTTAACAGAATT+TGG | - | chr5_2:69842974-69842993 | Msa0785020:five_prime_UTR | 30.0% | |
| ! | TAGTGATCATGTCTATCTTT+TGG | - | chr5_2:69843051-69843070 | Msa0785020:five_prime_UTR | 30.0% |
| TGAGTTCAAAAGGAAGAAGA+AGG | + | chr5_2:69840771-69840790 | None:intergenic | 35.0% | |
| AGAGTAGAAGTGAGTTCAAA+AGG | + | chr5_2:69840781-69840800 | None:intergenic | 35.0% | |
| AGGGTGGTGTATAATATTCT+TGG | - | chr5_2:69841094-69841113 | Msa0785020:CDS | 35.0% | |
| AAGAGAGTTACCAAACAGAA+TGG | - | chr5_2:69841451-69841470 | Msa0785020:intron | 35.0% | |
| ATAAGGATACACCAGATAGA+TGG | - | chr5_2:69841520-69841539 | Msa0785020:intron | 35.0% | |
| ACATCAAAAACAGTCTTACC+AGG | + | chr5_2:69841567-69841586 | None:intergenic | 35.0% | |
| GATTAAGCAGTATAGGGAAT+TGG | - | chr5_2:69841587-69841606 | Msa0785020:intron | 35.0% | |
| AGAAGATGTGAGTGAAATTG+AGG | - | chr5_2:69841611-69841630 | Msa0785020:intron | 35.0% | |
| AACTTCAAAGGTGAAAGAAG+AGG | + | chr5_2:69841667-69841686 | None:intergenic | 35.0% | |
| ! | ACTATGATGGAAACAGAAGA+AGG | - | chr5_2:69841700-69841719 | Msa0785020:intron | 35.0% |
| GAGTCTGTAACACTTAATGA+TGG | - | chr5_2:69841968-69841987 | Msa0785020:intron | 35.0% | |
| ATAAGGCAAAAGGTTTCTTC+TGG | - | chr5_2:69842609-69842628 | Msa0785020:five_prime_UTR | 35.0% | |
| GTTGTGATATCATGAATGCT+TGG | + | chr5_2:69842654-69842673 | None:intergenic | 35.0% | |
| ! | AGGAGACACATTGACTAATA+AGG | + | chr5_2:69842715-69842734 | None:intergenic | 35.0% |
| TATGCTAAGCTGAAAGTTTC+GGG | - | chr5_2:69842825-69842844 | Msa0785020:five_prime_UTR | 35.0% | |
| !! | TTCAACAAAGGAGCTGTTTT+TGG | - | chr5_2:69842870-69842889 | Msa0785020:five_prime_UTR | 35.0% |
| TCCTTTAACAGAATTTGGAG+TGG | - | chr5_2:69842979-69842998 | Msa0785020:five_prime_UTR | 35.0% | |
| TCCACTCCAAATTCTGTTAA+AGG | + | chr5_2:69842983-69843002 | None:intergenic | 35.0% | |
| ! | CACTTTGTCGTTTCTTCATG+AGG | + | chr5_2:69840876-69840895 | None:intergenic | 40.0% |
| AGGAACATTCAAGAGCAAGA+GGG | + | chr5_2:69840997-69841016 | None:intergenic | 40.0% | |
| TAGGAACATTCAAGAGCAAG+AGG | + | chr5_2:69840998-69841017 | None:intergenic | 40.0% | |
| ! | TCAACCTTTCTTTAAGAGGG+TGG | - | chr5_2:69841078-69841097 | Msa0785020:CDS | 40.0% |
| TACACCACCCTCTTAAAGAA+AGG | + | chr5_2:69841085-69841104 | None:intergenic | 40.0% | |
| CTTCTCTACTCCATTCTGTT+TGG | + | chr5_2:69841464-69841483 | None:intergenic | 40.0% | |
| GCAATCTTAAGCCATCTATC+TGG | + | chr5_2:69841534-69841553 | None:intergenic | 40.0% | |
| AAGATTGCTGAGATGATTCC+TGG | - | chr5_2:69841546-69841565 | Msa0785020:intron | 40.0% | |
| AAAGAAGAGGCAAGATAACC+AGG | + | chr5_2:69841654-69841673 | None:intergenic | 40.0% | |
| ! | TTAGAGGTTCTGATCATGAG+AGG | - | chr5_2:69841733-69841752 | Msa0785020:intron | 40.0% |
| TCTGATCATGAGAGGAAGAA+AGG | - | chr5_2:69841741-69841760 | Msa0785020:intron | 40.0% | |
| GTAAAGGCAAACTCCAATTG+AGG | + | chr5_2:69842280-69842299 | None:intergenic | 40.0% | |
| ! | ACGTACAGACGTTTTCTGAT+GGG | - | chr5_2:69842486-69842505 | Msa0785020:CDS | 40.0% |
| !!! | CTGATGGGGCTTTTAAAGTA+TGG | - | chr5_2:69842501-69842520 | Msa0785020:CDS | 40.0% |
| !!! | GGGCTTTTAAAGTATGGCAA+AGG | - | chr5_2:69842507-69842526 | Msa0785020:CDS | 40.0% |
| TCTCCTATGCAGCAGAAAAT+GGG | - | chr5_2:69842729-69842748 | Msa0785020:five_prime_UTR | 40.0% | |
| AGTACTAGCCACTACAATGA+TGG | - | chr5_2:69842756-69842775 | Msa0785020:five_prime_UTR | 40.0% | |
| !! | TACAATGATGGATCACCACA+AGG | - | chr5_2:69842768-69842787 | Msa0785020:five_prime_UTR | 40.0% |
| !! | ACAATGATGGATCACCACAA+GGG | - | chr5_2:69842769-69842788 | Msa0785020:five_prime_UTR | 40.0% |
| ! | GCTTCTAGAGACTTCAACAA+AGG | - | chr5_2:69842858-69842877 | Msa0785020:five_prime_UTR | 40.0% |
| !! | GTTGATGACATGAAGCTACT+TGG | - | chr5_2:69842910-69842929 | Msa0785020:five_prime_UTR | 40.0% |
| AAGAGCAAGAGGGTGAAAGA+GGG | + | chr5_2:69840987-69841006 | None:intergenic | 45.0% | |
| GATGTGAGTGAAATTGAGGC+CGG | - | chr5_2:69841615-69841634 | Msa0785020:intron | 45.0% | |
| ACCAGGAATCGGAACATTTC+CGG | + | chr5_2:69841637-69841656 | None:intergenic | 45.0% | |
| GAGGCAAGATAACCAGGAAT+CGG | + | chr5_2:69841648-69841667 | None:intergenic | 45.0% | |
| ! | AGAAGGCATGTGACTGTTAG+AGG | - | chr5_2:69841717-69841736 | Msa0785020:intron | 45.0% |
| !! | AGAGGAAGAAAGGTGTTCCT+TGG | - | chr5_2:69841751-69841770 | Msa0785020:intron | 45.0% |
| CTATGTTCCTCTTCAGTCCA+AGG | + | chr5_2:69841771-69841790 | None:intergenic | 45.0% | |
| CTTGGACTGAAGAGGAACAT+AGG | - | chr5_2:69841769-69841788 | Msa0785020:intron | 45.0% | |
| TAAGACACGGAAGCTAGAGA+CGG | - | chr5_2:69841919-69841938 | Msa0785020:intron | 45.0% | |
| GCAGATAATGCAGCCTCAAT+TGG | - | chr5_2:69842264-69842283 | Msa0785020:CDS | 45.0% | |
| ! | GACGTACAGACGTTTTCTGA+TGG | - | chr5_2:69842485-69842504 | Msa0785020:CDS | 45.0% |
| ! | CGTACAGACGTTTTCTGATG+GGG | - | chr5_2:69842487-69842506 | Msa0785020:CDS | 45.0% |
| TAAAGTATGGCAAAGGCGAC+TGG | - | chr5_2:69842514-69842533 | Msa0785020:CDS | 45.0% | |
| !! | GCATGACTTGCTACTTGTGT+TGG | + | chr5_2:69842576-69842595 | None:intergenic | 45.0% |
| AGGCAAAAGGTTTCTTCTGG+AGG | - | chr5_2:69842612-69842631 | Msa0785020:five_prime_UTR | 45.0% | |
| GTCTCCTATGCAGCAGAAAA+TGG | - | chr5_2:69842728-69842747 | Msa0785020:five_prime_UTR | 45.0% | |
| ! | CAACCCATTTTCTGCTGCAT+AGG | + | chr5_2:69842735-69842754 | None:intergenic | 45.0% |
| CTATGCAGCAGAAAATGGGT+TGG | - | chr5_2:69842733-69842752 | Msa0785020:five_prime_UTR | 45.0% | |
| GTGGTGATCCATCATTGTAG+TGG | + | chr5_2:69842767-69842786 | None:intergenic | 45.0% | |
| CTATGACTGCTCTTTCCATG+AGG | - | chr5_2:69842800-69842819 | Msa0785020:five_prime_UTR | 45.0% | |
| TCAGCTTAGCATAAGCCTCA+TGG | + | chr5_2:69842818-69842837 | None:intergenic | 45.0% | |
| GAGACACAGCATGTCTGAAT+GGG | + | chr5_2:69843102-69843121 | None:intergenic | 45.0% | |
| TGAGACACAGCATGTCTGAA+TGG | + | chr5_2:69843103-69843122 | None:intergenic | 45.0% | |
| CAAGAGCAAGAGGGTGAAAG+AGG | + | chr5_2:69840988-69841007 | None:intergenic | 50.0% | |
| AGGTGTTCCTTGGACTGAAG+AGG | - | chr5_2:69841761-69841780 | Msa0785020:intron | 50.0% | |
| TCATAGAGATCCTGCCCTTG+TGG | + | chr5_2:69842786-69842805 | None:intergenic | 50.0% | |
| GCCGGAAATGTTCCGATTCC+TGG | - | chr5_2:69841633-69841652 | Msa0785020:intron | 55.0% | |
| ! | TGATGGATCACCACAAGGGC+AGG | - | chr5_2:69842773-69842792 | Msa0785020:five_prime_UTR | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 69840728 | 69843229 | 69840728 | ID=Msa0785020;Name=Msa0785020 |
| chr5_2 | mRNA | 69840728 | 69843229 | 69840728 | ID=Msa0785020-mRNA-1;Parent=Msa0785020;Name=Msa0785020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=665|1|1|1|1|1|2|321|270 |
| chr5_2 | exon | 69840728 | 69841463 | 69840728 | ID=Msa0785020-mRNA-1:exon:21031;Parent=Msa0785020-mRNA-1 |
| chr5_2 | exon | 69842167 | 69843229 | 69842167 | ID=Msa0785020-mRNA-1:exon:21030;Parent=Msa0785020-mRNA-1 |
| chr5_2 | five_prime_UTR | 69842565 | 69843229 | 69842565 | ID=Msa0785020-mRNA-1:five_prime_utr;Parent=Msa0785020-mRNA-1 |
| chr5_2 | CDS | 69842167 | 69842564 | 69842167 | ID=Msa0785020-mRNA-1:cds;Parent=Msa0785020-mRNA-1 |
| chr5_2 | CDS | 69841049 | 69841463 | 69841049 | ID=Msa0785020-mRNA-1:cds;Parent=Msa0785020-mRNA-1 |
| chr5_2 | three_prime_UTR | 69840728 | 69841048 | 69840728 | ID=Msa0785020-mRNA-1:three_prime_utr;Parent=Msa0785020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
