AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0785660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0785660 MtrunA17_Chr5g0435641 96.354 192 6 1 1 191 1 192 5.03e-138 383
Msa0785660 MtrunA17_Chr7g0261031 63.333 120 44 0 1 120 2 121 3.73e-53 169
Msa0785660 MtrunA17_Chr1g0184271 70.588 102 30 0 8 109 13 114 2.41e-50 162
Msa0785660 MtrunA17_Chr7g0260971 65.000 100 35 0 2 101 9 108 2.81e-46 151
Msa0785660 MtrunA17_Chr6g0460951 54.622 119 54 0 6 124 39 157 3.27e-45 149
Msa0785660 MtrunA17_Chr8g0351721 60.377 106 42 0 5 110 16 121 9.70e-45 147
Msa0785660 MtrunA17_Chr8g0351701 63.208 106 39 0 8 113 24 129 1.03e-44 148
Msa0785660 MtrunA17_Chr1g0184281 49.306 144 60 2 5 148 13 143 3.52e-43 143
Msa0785660 MtrunA17_Chr7g0245871 65.657 99 34 0 8 106 28 126 1.71e-42 142
Msa0785660 MtrunA17_Chr7g0245861 64.130 92 31 2 5 94 10 101 9.71e-39 129
Msa0785660 MtrunA17_Chr7g0227511 55.670 97 42 1 6 102 10 105 2.85e-34 119
Msa0785660 MtrunA17_Chr2g0311601 55.556 99 43 1 3 101 9 106 7.82e-34 118
Msa0785660 MtrunA17_Chr3g0114061 54.082 98 44 1 6 103 7 103 2.36e-33 116
Msa0785660 MtrunA17_Chr5g0437571 38.824 170 77 4 3 167 2 149 3.90e-33 116
Msa0785660 MtrunA17_Chr4g0058651 47.368 114 59 1 4 117 8 120 5.65e-33 116
Msa0785660 MtrunA17_Chr4g0043421 51.485 101 48 1 3 103 11 110 1.42e-32 115
Msa0785660 MtrunA17_Chr6g0449641 52.577 97 45 1 6 102 8 103 1.93e-32 114
Msa0785660 MtrunA17_Chr3g0117121 51.485 101 48 1 2 102 5 104 3.59e-32 114
Msa0785660 MtrunA17_Chr3g0106101 47.934 121 56 3 3 117 2 121 3.82e-32 114
Msa0785660 MtrunA17_Chr3g0129071 52.525 99 46 1 6 104 46 143 5.01e-31 113
Msa0785660 MtrunA17_Chr3g0097221 37.870 169 87 5 1 160 1 160 5.50e-31 114
Msa0785660 MtrunA17_Chr5g0438091 51.515 99 47 1 6 104 38 135 5.56e-31 111
Msa0785660 MtrunA17_Chr5g0437401 44.340 106 58 1 5 110 9 113 1.47e-30 110
Msa0785660 MtrunA17_Chr6g0460941 53.191 94 44 0 1 94 1 94 2.33e-30 107
Msa0785660 MtrunA17_Chr3g0113891 47.959 98 50 1 6 103 13 109 3.15e-30 109
Msa0785660 MtrunA17_Chr6g0449651 51.546 97 46 1 6 102 66 161 3.31e-30 109
Msa0785660 MtrunA17_Chr3g0115431 46.667 105 55 1 5 109 7 110 1.07e-29 109
Msa0785660 MtrunA17_Chr5g0402611 51.546 97 46 1 6 102 7 102 3.43e-29 110
Msa0785660 MtrunA17_Chr4g0030001 46.018 113 52 3 6 117 23 127 1.46e-27 102
Msa0785660 MtrunA17_Chr1g0200201 46.809 94 49 1 8 101 73 165 1.40e-25 98.6
Msa0785660 MtrunA17_Chr5g0432451 53.659 82 37 1 6 87 42 122 1.44e-25 98.6
Msa0785660 MtrunA17_Chr8g0353981 50.575 87 42 1 1 87 1 86 5.66e-25 97.1
Msa0785660 MtrunA17_Chr7g0225111 43.269 104 58 1 6 109 4 106 1.01e-24 93.2
Msa0785660 MtrunA17_Chr5g0444351 43.137 102 57 1 8 109 6 106 2.46e-24 93.2
Msa0785660 MtrunA17_Chr8g0342351 35.922 103 65 1 7 109 12 113 1.45e-19 83.6
Msa0785660 MtrunA17_Chr4g0040831 32.331 133 88 2 3 135 11 141 1.82e-19 82.0
Msa0785660 MtrunA17_Chr4g0059901 38.384 99 60 1 3 101 6 103 3.27e-19 82.8
Msa0785660 MtrunA17_Chr6g0452691 35.922 103 64 2 8 109 10 111 1.39e-17 76.6
Msa0785660 MtrunA17_Chr8g0374601 37.255 102 63 1 8 109 27 127 1.87e-17 78.2
Msa0785660 MtrunA17_Chr8g0374591 37.864 103 63 1 8 110 9 110 1.94e-17 74.7
Msa0785660 MtrunA17_Chr6g0452671 39.506 81 48 1 7 87 11 90 3.20e-16 73.6
Msa0785660 MtrunA17_Chr2g0330201 31.683 101 68 1 3 103 15 114 5.30e-16 74.3
Msa0785660 MtrunA17_Chr6g0452661 32.692 104 68 2 7 109 9 111 7.50e-16 73.2
Msa0785660 MtrunA17_Chr5g0439111 33.654 104 67 2 7 109 9 111 2.19e-14 68.9
Msa0785660 MtrunA17_Chr8g0374581 35.789 95 59 2 8 101 9 102 6.20e-14 67.8
Msa0785660 MtrunA17_Chr4g0055441 30.097 103 70 2 8 109 3 104 3.36e-13 64.3
Msa0785660 MtrunA17_Chr2g0326041 52.083 48 22 1 6 53 32 78 1.86e-12 60.5
Msa0785660 MtrunA17_Chr4g0046201 31.111 90 61 1 6 95 5 93 2.67e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0785660 AT5G06080.1 51.596 188 72 4 1 186 1 171 3.54e-59 183
Msa0785660 AT3G58190.1 69.811 106 32 0 8 113 12 117 1.13e-51 166
Msa0785660 AT2G42440.1 61.475 122 42 1 1 117 1 122 2.21e-50 163
Msa0785660 AT2G42430.1 45.856 181 75 3 3 166 11 185 8.90e-48 156
Msa0785660 AT3G03760.1 61.607 112 43 0 6 117 50 161 5.34e-47 155
Msa0785660 AT2G31310.1 63.462 104 38 0 1 104 1 104 1.20e-45 149
Msa0785660 AT4G00210.1 63.000 100 37 0 2 101 6 105 4.18e-45 149
Msa0785660 AT2G45420.1 65.347 101 35 0 10 110 40 140 5.44e-44 147
Msa0785660 AT4G00220.1 61.386 101 39 0 10 110 20 120 9.73e-42 140
Msa0785660 AT2G45410.1 58.696 92 38 0 10 101 19 110 4.59e-37 127
Msa0785660 AT3G27650.1 55.670 97 42 1 6 102 38 133 2.08e-34 119
Msa0785660 AT5G63090.4 48.276 116 59 1 4 119 8 122 1.80e-33 118
Msa0785660 AT5G63090.3 48.276 116 59 1 4 119 8 122 1.80e-33 118
Msa0785660 AT5G63090.2 48.276 116 59 1 4 119 8 122 1.80e-33 118
Msa0785660 AT5G63090.1 48.276 116 59 1 4 119 8 122 1.80e-33 118
Msa0785660 AT2G30130.1 51.923 104 48 2 1 103 1 103 4.38e-32 114
Msa0785660 AT1G31320.1 54.348 92 41 1 3 94 9 99 1.35e-31 113
Msa0785660 AT2G28500.1 45.133 113 56 2 6 113 54 165 2.20e-29 108
Msa0785660 AT3G11090.1 44.037 109 59 2 6 113 10 117 3.65e-29 106
Msa0785660 AT1G16530.1 38.462 143 84 3 6 147 13 152 6.96e-29 105
Msa0785660 AT1G07900.1 45.536 112 53 2 6 117 32 135 7.90e-29 106
Msa0785660 AT3G26660.1 45.833 96 51 1 6 101 4 98 1.10e-28 103
Msa0785660 AT5G66870.1 49.020 102 51 1 1 102 1 101 1.30e-28 108
Msa0785660 AT3G26620.1 45.833 96 51 1 6 101 4 98 1.63e-28 103
Msa0785660 AT2G30340.2 50.505 99 48 1 6 104 52 149 3.43e-28 106
Msa0785660 AT2G30340.1 50.505 99 48 1 6 104 51 148 4.47e-28 106
Msa0785660 AT2G23660.2 48.958 96 48 1 6 101 4 98 2.73e-27 105
Msa0785660 AT2G23660.3 48.958 96 48 1 6 101 4 98 2.73e-27 105
Msa0785660 AT2G23660.1 48.958 96 48 1 6 101 4 98 2.73e-27 105
Msa0785660 AT1G65620.1 56.098 82 35 1 6 87 8 88 5.44e-26 99.4
Msa0785660 AT1G65620.4 56.098 82 35 1 6 87 8 88 5.44e-26 99.4
Msa0785660 AT1G65620.3 56.098 82 35 1 6 87 8 88 5.44e-26 99.4
Msa0785660 AT1G65620.5 56.098 82 35 1 6 87 8 88 5.44e-26 99.4
Msa0785660 AT1G65620.2 56.098 82 35 1 6 87 8 88 5.44e-26 99.4
Msa0785660 AT2G40470.2 51.220 82 39 1 6 87 19 99 5.00e-25 97.1
Msa0785660 AT2G40470.1 51.220 82 39 1 6 87 44 124 7.32e-25 97.1
Msa0785660 AT3G50510.1 40.708 113 66 1 1 113 6 117 2.86e-24 94.7
Msa0785660 AT3G50510.2 40.708 113 66 1 1 113 6 117 2.86e-24 94.7
Msa0785660 AT3G13850.1 36.893 103 64 1 7 109 36 137 2.19e-19 83.6
Msa0785660 AT5G35900.1 39.796 98 56 3 6 102 4 99 7.29e-18 78.2
Msa0785660 AT3G47870.1 32.075 106 71 1 3 108 32 136 5.12e-17 77.8
Msa0785660 AT1G06280.1 36.842 76 47 1 7 82 24 98 4.41e-16 73.6
Msa0785660 AT1G72980.1 31.683 101 68 1 3 103 9 108 1.21e-13 67.0
Msa0785660 AT4G22700.1 33.333 96 61 2 8 102 6 99 5.88e-12 62.0

Find 40 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCAGTGCATAAGGTTTA+TGG 0.153421 5_2:-70772154 Msa0785660:CDS
CGCATGCAAGATCCTATTTA+TGG 0.202738 5_2:-70772043 Msa0785660:CDS
CTTTGGAAAATGACAGGATT+TGG 0.236785 5_2:-70772274 None:intergenic
ATGATCCAAAACTTTGATTA+TGG 0.255926 5_2:+70771222 None:intergenic
TATTACCATAATCAAAGTTT+TGG 0.272555 5_2:-70771227 Msa0785660:CDS
CAAGAGGAGATAGATATTCT+TGG 0.321470 5_2:-70771332 Msa0785660:CDS
GGATTTCCAGAGCCTTTATA+TGG 0.325475 5_2:-70771167 Msa0785660:CDS
CAGCAGTGCATAAGGTTTAT+GGG 0.335701 5_2:-70772153 Msa0785660:CDS
TCTGGAAATCCTTGTGCATT+AGG 0.337625 5_2:+70771179 None:intergenic
ACATTAGAGAAGTTCTTATC+AGG 0.342216 5_2:-70771128 Msa0785660:CDS
TGAGCAACACAACCATAAAT+AGG 0.353770 5_2:+70772031 None:intergenic
GCACCAATGAACTCAAATAA+TGG 0.356387 5_2:-70771257 Msa0785660:CDS
CACCCTTGGTTCAAGCATAA+TGG 0.370662 5_2:-70771080 Msa0785660:CDS
GTTCCATTATTTGAGTTCAT+TGG 0.371818 5_2:+70771254 None:intergenic
GCCTGTTCATAGGAGAAGTA+TGG 0.380890 5_2:+70772190 None:intergenic
GAGTTGGAATCACCATATAA+AGG 0.394155 5_2:+70771155 None:intergenic
TCCATACTTCTCCTATGAAC+AGG 0.425422 5_2:-70772191 Msa0785660:CDS
CTTCTCTAATGTATTTGAGT+TGG 0.427053 5_2:+70771139 None:intergenic
CACTATATCTTATGAAGCCT+TGG 0.435812 5_2:-70772068 Msa0785660:CDS
ATGAGTTGAAGCCTGTTCAT+AGG 0.437710 5_2:+70772180 None:intergenic
GAATCACCATATAAAGGCTC+TGG 0.448083 5_2:+70771161 None:intergenic
CACTAGCACACTTTCTCCTT+AGG 0.465254 5_2:+70772227 None:intergenic
AGGGCTTCTTGAACCACCCT+TGG 0.469455 5_2:-70771094 Msa0785660:CDS
ATAAAACTTTGGAAAATGAC+AGG 0.478814 5_2:-70772280 None:intergenic
TATCTATGCATTGCAACAAC+AGG 0.479361 5_2:-70772008 Msa0785660:intron
GTGGAGCATGCAAGTTCCTA+AGG 0.513337 5_2:-70772243 Msa0785660:CDS
CTCCATTATGCTTGAACCAA+GGG 0.523653 5_2:+70771078 None:intergenic
AGGATCTTGCATGCGAGCCA+AGG 0.545581 5_2:+70772051 None:intergenic
AGATATTCTTGGAAGTGTAA+TGG 0.550072 5_2:-70771321 Msa0785660:CDS
TCTCCATTATGCTTGAACCA+AGG 0.563541 5_2:+70771077 None:intergenic
TAATGGAGACATGAAGAACT+AGG 0.571172 5_2:-70771063 None:intergenic
AGCAGTGCATAAGGTTTATG+GGG 0.583924 5_2:-70772152 Msa0785660:CDS
ACAGGATTTGGCTCATCATG+TGG 0.589293 5_2:-70772262 Msa0785660:CDS
CATTATGCTTGAACCAAGGG+TGG 0.607660 5_2:+70771081 None:intergenic
AGTGTTAGTGTTGTGAATTG+TGG 0.611640 5_2:-70771290 Msa0785660:CDS
GCAGGTTGCTAGCTTGCAAG+AGG 0.615316 5_2:-70771348 Msa0785660:intron
CTTATCAGGAATTGATCAAG+AGG 0.617949 5_2:-70771114 Msa0785660:CDS
TTATCAGGAATTGATCAAGA+GGG 0.643489 5_2:-70771113 Msa0785660:CDS
GGCTTCATAAGATATAGTGA+TGG 0.646623 5_2:+70772072 None:intergenic
GAGCTAATTCCTAATGCACA+AGG 0.651993 5_2:-70771188 Msa0785660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTGTTTCAAAAAATTA+CGG + chr5_2:70771629-70771648 None:intergenic 10.0%
!! TTAATTGTTTCAAAAAATTA+CGG + chr5_2:70771629-70771648 None:intergenic 10.0%
!!! ATTTTTTGTCAATATCATGA+TGG - chr5_2:70771653-70771672 Msa0785660:intron 20.0%
!!! TATTACCATAATCAAAGTTT+TGG - chr5_2:70772102-70772121 Msa0785660:CDS 20.0%
!!! ATTTTTTGTCAATATCATGA+TGG - chr5_2:70771653-70771672 Msa0785660:intron 20.0%
!!! TATTACCATAATCAAAGTTT+TGG - chr5_2:70772102-70772121 Msa0785660:CDS 20.0%
!!! ACAACCATTGTTTTAAATTG+TGG + chr5_2:70771511-70771530 None:intergenic 25.0%
! ATTGTTTCAAAAAATTACGG+TGG + chr5_2:70771626-70771645 None:intergenic 25.0%
! ATGCATCAATATATAAGCAA+TGG + chr5_2:70771710-70771729 None:intergenic 25.0%
!!! GCTTGTTATAAATAAGAAGT+TGG - chr5_2:70771837-70771856 Msa0785660:intron 25.0%
! ATGATCCAAAACTTTGATTA+TGG + chr5_2:70772110-70772129 None:intergenic 25.0%
!!! ACAACCATTGTTTTAAATTG+TGG + chr5_2:70771511-70771530 None:intergenic 25.0%
! ATTGTTTCAAAAAATTACGG+TGG + chr5_2:70771626-70771645 None:intergenic 25.0%
! ATGCATCAATATATAAGCAA+TGG + chr5_2:70771710-70771729 None:intergenic 25.0%
!!! GCTTGTTATAAATAAGAAGT+TGG - chr5_2:70771837-70771856 Msa0785660:intron 25.0%
! ATGATCCAAAACTTTGATTA+TGG + chr5_2:70772110-70772129 None:intergenic 25.0%
!! GCATCACTTCTATTTTGAAT+AGG + chr5_2:70771235-70771254 None:intergenic 30.0%
CTTTATATTGCACCAAAATG+CGG - chr5_2:70771400-70771419 Msa0785660:intron 30.0%
! AATTTAAAACCTTGGTTGTG+AGG + chr5_2:70771536-70771555 None:intergenic 30.0%
TAAATTGTCGAGCAAAATCA+TGG - chr5_2:70771550-70771569 Msa0785660:intron 30.0%
AATGGATGCTAGAAATATAG+CGG + chr5_2:70771692-70771711 None:intergenic 30.0%
ATTCAATGCATGTGTAAGTA+AGG + chr5_2:70771795-70771814 None:intergenic 30.0%
CTCTCCACAAAAAATTTCTT+AGG - chr5_2:70771872-70771891 Msa0785660:intron 30.0%
TTACAATTATGTGTAGGTCA+TGG + chr5_2:70771917-70771936 None:intergenic 30.0%
ATGACCTACACATAATTGTA+AGG - chr5_2:70771916-70771935 Msa0785660:intron 30.0%
TGACCTACACATAATTGTAA+GGG - chr5_2:70771917-70771936 Msa0785660:intron 30.0%
! ACATAATTGTAAGGGTGTAA+TGG - chr5_2:70771925-70771944 Msa0785660:intron 30.0%
! AGATATTCTTGGAAGTGTAA+TGG - chr5_2:70772008-70772027 Msa0785660:intron 30.0%
! GTTCCATTATTTGAGTTCAT+TGG + chr5_2:70772078-70772097 None:intergenic 30.0%
!!! TCAAAGTTTTGGATCATACT+TGG - chr5_2:70772113-70772132 Msa0785660:CDS 30.0%
! TTTTGGATCATACTTGGATA+TGG - chr5_2:70772119-70772138 Msa0785660:CDS 30.0%
CTTCTCTAATGTATTTGAGT+TGG + chr5_2:70772193-70772212 None:intergenic 30.0%
ACATTAGAGAAGTTCTTATC+AGG - chr5_2:70772201-70772220 Msa0785660:CDS 30.0%
TTATCAGGAATTGATCAAGA+GGG - chr5_2:70772216-70772235 Msa0785660:CDS 30.0%
!! GCATCACTTCTATTTTGAAT+AGG + chr5_2:70771235-70771254 None:intergenic 30.0%
CTTTATATTGCACCAAAATG+CGG - chr5_2:70771400-70771419 Msa0785660:intron 30.0%
! AATTTAAAACCTTGGTTGTG+AGG + chr5_2:70771536-70771555 None:intergenic 30.0%
TAAATTGTCGAGCAAAATCA+TGG - chr5_2:70771550-70771569 Msa0785660:intron 30.0%
AATGGATGCTAGAAATATAG+CGG + chr5_2:70771692-70771711 None:intergenic 30.0%
ATTCAATGCATGTGTAAGTA+AGG + chr5_2:70771795-70771814 None:intergenic 30.0%
CTCTCCACAAAAAATTTCTT+AGG - chr5_2:70771872-70771891 Msa0785660:intron 30.0%
TTACAATTATGTGTAGGTCA+TGG + chr5_2:70771917-70771936 None:intergenic 30.0%
ATGACCTACACATAATTGTA+AGG - chr5_2:70771916-70771935 Msa0785660:intron 30.0%
TGACCTACACATAATTGTAA+GGG - chr5_2:70771917-70771936 Msa0785660:intron 30.0%
! ACATAATTGTAAGGGTGTAA+TGG - chr5_2:70771925-70771944 Msa0785660:intron 30.0%
! AGATATTCTTGGAAGTGTAA+TGG - chr5_2:70772008-70772027 Msa0785660:intron 30.0%
! GTTCCATTATTTGAGTTCAT+TGG + chr5_2:70772078-70772097 None:intergenic 30.0%
!!! TCAAAGTTTTGGATCATACT+TGG - chr5_2:70772113-70772132 Msa0785660:CDS 30.0%
! TTTTGGATCATACTTGGATA+TGG - chr5_2:70772119-70772138 Msa0785660:CDS 30.0%
CTTCTCTAATGTATTTGAGT+TGG + chr5_2:70772193-70772212 None:intergenic 30.0%
ACATTAGAGAAGTTCTTATC+AGG - chr5_2:70772201-70772220 Msa0785660:CDS 30.0%
TTATCAGGAATTGATCAAGA+GGG - chr5_2:70772216-70772235 Msa0785660:CDS 30.0%
!! TCATTTTTCAGCAGTGCATA+AGG - chr5_2:70771168-70771187 Msa0785660:CDS 35.0%
GGCTTCATAAGATATAGTGA+TGG + chr5_2:70771260-70771279 None:intergenic 35.0%
CACTATATCTTATGAAGCCT+TGG - chr5_2:70771261-70771280 Msa0785660:CDS 35.0%
TGAGCAACACAACCATAAAT+AGG + chr5_2:70771301-70771320 None:intergenic 35.0%
TATCTATGCATTGCAACAAC+AGG - chr5_2:70771321-70771340 Msa0785660:CDS 35.0%
CACCAAAATGCGGTAAAATA+TGG - chr5_2:70771410-70771429 Msa0785660:intron 35.0%
TGTGGATGTTAGTTAGATTG+TGG - chr5_2:70771466-70771485 Msa0785660:intron 35.0%
! CGGACCACAATTTAAAACAA+TGG - chr5_2:70771504-70771523 Msa0785660:intron 35.0%
! TGCTCGACAATTTAAAACCT+TGG + chr5_2:70771544-70771563 None:intergenic 35.0%
GAGCAAAATCATGGATATTG+TGG - chr5_2:70771559-70771578 Msa0785660:intron 35.0%
ATATGCAGATAATGTGACCT+TGG - chr5_2:70771584-70771603 Msa0785660:intron 35.0%
AATGCATGTGTAAGTAAGGA+TGG + chr5_2:70771791-70771810 None:intergenic 35.0%
!!! GAGGCCTAAGAAATTTTTTG+TGG + chr5_2:70771879-70771898 None:intergenic 35.0%
ACACCCTTACAATTATGTGT+AGG + chr5_2:70771923-70771942 None:intergenic 35.0%
TTTCTTATGTGTGATTGTGC+AGG - chr5_2:70771963-70771982 Msa0785660:intron 35.0%
CAAGAGGAGATAGATATTCT+TGG - chr5_2:70771997-70772016 Msa0785660:intron 35.0%
AGTGTTAGTGTTGTGAATTG+TGG - chr5_2:70772039-70772058 Msa0785660:CDS 35.0%
GCACCAATGAACTCAAATAA+TGG - chr5_2:70772072-70772091 Msa0785660:CDS 35.0%
GAGTTGGAATCACCATATAA+AGG + chr5_2:70772177-70772196 None:intergenic 35.0%
CTTATCAGGAATTGATCAAG+AGG - chr5_2:70772215-70772234 Msa0785660:CDS 35.0%
!! TCATTTTTCAGCAGTGCATA+AGG - chr5_2:70771168-70771187 Msa0785660:CDS 35.0%
GGCTTCATAAGATATAGTGA+TGG + chr5_2:70771260-70771279 None:intergenic 35.0%
CACTATATCTTATGAAGCCT+TGG - chr5_2:70771261-70771280 Msa0785660:CDS 35.0%
TGAGCAACACAACCATAAAT+AGG + chr5_2:70771301-70771320 None:intergenic 35.0%
TATCTATGCATTGCAACAAC+AGG - chr5_2:70771321-70771340 Msa0785660:CDS 35.0%
CACCAAAATGCGGTAAAATA+TGG - chr5_2:70771410-70771429 Msa0785660:intron 35.0%
TGTGGATGTTAGTTAGATTG+TGG - chr5_2:70771466-70771485 Msa0785660:intron 35.0%
! CGGACCACAATTTAAAACAA+TGG - chr5_2:70771504-70771523 Msa0785660:intron 35.0%
! TGCTCGACAATTTAAAACCT+TGG + chr5_2:70771544-70771563 None:intergenic 35.0%
GAGCAAAATCATGGATATTG+TGG - chr5_2:70771559-70771578 Msa0785660:intron 35.0%
ATATGCAGATAATGTGACCT+TGG - chr5_2:70771584-70771603 Msa0785660:intron 35.0%
AATGCATGTGTAAGTAAGGA+TGG + chr5_2:70771791-70771810 None:intergenic 35.0%
!!! GAGGCCTAAGAAATTTTTTG+TGG + chr5_2:70771879-70771898 None:intergenic 35.0%
ACACCCTTACAATTATGTGT+AGG + chr5_2:70771923-70771942 None:intergenic 35.0%
TTTCTTATGTGTGATTGTGC+AGG - chr5_2:70771963-70771982 Msa0785660:intron 35.0%
CAAGAGGAGATAGATATTCT+TGG - chr5_2:70771997-70772016 Msa0785660:intron 35.0%
AGTGTTAGTGTTGTGAATTG+TGG - chr5_2:70772039-70772058 Msa0785660:CDS 35.0%
GCACCAATGAACTCAAATAA+TGG - chr5_2:70772072-70772091 Msa0785660:CDS 35.0%
GAGTTGGAATCACCATATAA+AGG + chr5_2:70772177-70772196 None:intergenic 35.0%
CTTATCAGGAATTGATCAAG+AGG - chr5_2:70772215-70772234 Msa0785660:CDS 35.0%
TCCATACTTCTCCTATGAAC+AGG - chr5_2:70771138-70771157 Msa0785660:CDS 40.0%
! ATGAGTTGAAGCCTGTTCAT+AGG + chr5_2:70771152-70771171 None:intergenic 40.0%
TCAGCAGTGCATAAGGTTTA+TGG - chr5_2:70771175-70771194 Msa0785660:CDS 40.0%
CAGCAGTGCATAAGGTTTAT+GGG - chr5_2:70771176-70771195 Msa0785660:CDS 40.0%
AGCAGTGCATAAGGTTTATG+GGG - chr5_2:70771177-70771196 Msa0785660:CDS 40.0%
CGCATGCAAGATCCTATTTA+TGG - chr5_2:70771286-70771305 Msa0785660:CDS 40.0%
! GGCCATATTTTACCGCATTT+TGG + chr5_2:70771415-70771434 None:intergenic 40.0%
!! AATCGCAATTTTGACCGCAT+GGG + chr5_2:70771436-70771455 None:intergenic 40.0%
TTGCGATTGTAATGTCGTTG+TGG - chr5_2:70771448-70771467 Msa0785660:intron 40.0%
! ATGTGACCTTGGTGCATATT+TGG - chr5_2:70771595-70771614 Msa0785660:intron 40.0%
GATCCAGAAAGTTGGAGTAA+TGG + chr5_2:70771769-70771788 None:intergenic 40.0%
TAAGGATGGATCCAGAAAGT+TGG + chr5_2:70771777-70771796 None:intergenic 40.0%
TAGGCCTCAAATTCTCTACA+CGG - chr5_2:70771891-70771910 Msa0785660:intron 40.0%
GAGCTAATTCCTAATGCACA+AGG - chr5_2:70772141-70772160 Msa0785660:CDS 40.0%
TCTGGAAATCCTTGTGCATT+AGG + chr5_2:70772153-70772172 None:intergenic 40.0%
GGATTTCCAGAGCCTTTATA+TGG - chr5_2:70772162-70772181 Msa0785660:CDS 40.0%
GAATCACCATATAAAGGCTC+TGG + chr5_2:70772171-70772190 None:intergenic 40.0%
CTCCATTATGCTTGAACCAA+GGG + chr5_2:70772254-70772273 None:intergenic 40.0%
TCTCCATTATGCTTGAACCA+AGG + chr5_2:70772255-70772274 None:intergenic 40.0%
TCCATACTTCTCCTATGAAC+AGG - chr5_2:70771138-70771157 Msa0785660:CDS 40.0%
! ATGAGTTGAAGCCTGTTCAT+AGG + chr5_2:70771152-70771171 None:intergenic 40.0%
TCAGCAGTGCATAAGGTTTA+TGG - chr5_2:70771175-70771194 Msa0785660:CDS 40.0%
CAGCAGTGCATAAGGTTTAT+GGG - chr5_2:70771176-70771195 Msa0785660:CDS 40.0%
AGCAGTGCATAAGGTTTATG+GGG - chr5_2:70771177-70771196 Msa0785660:CDS 40.0%
CGCATGCAAGATCCTATTTA+TGG - chr5_2:70771286-70771305 Msa0785660:CDS 40.0%
! GGCCATATTTTACCGCATTT+TGG + chr5_2:70771415-70771434 None:intergenic 40.0%
!! AATCGCAATTTTGACCGCAT+GGG + chr5_2:70771436-70771455 None:intergenic 40.0%
TTGCGATTGTAATGTCGTTG+TGG - chr5_2:70771448-70771467 Msa0785660:intron 40.0%
! ATGTGACCTTGGTGCATATT+TGG - chr5_2:70771595-70771614 Msa0785660:intron 40.0%
GATCCAGAAAGTTGGAGTAA+TGG + chr5_2:70771769-70771788 None:intergenic 40.0%
TAAGGATGGATCCAGAAAGT+TGG + chr5_2:70771777-70771796 None:intergenic 40.0%
TAGGCCTCAAATTCTCTACA+CGG - chr5_2:70771891-70771910 Msa0785660:intron 40.0%
GAGCTAATTCCTAATGCACA+AGG - chr5_2:70772141-70772160 Msa0785660:CDS 40.0%
TCTGGAAATCCTTGTGCATT+AGG + chr5_2:70772153-70772172 None:intergenic 40.0%
GGATTTCCAGAGCCTTTATA+TGG - chr5_2:70772162-70772181 Msa0785660:CDS 40.0%
GAATCACCATATAAAGGCTC+TGG + chr5_2:70772171-70772190 None:intergenic 40.0%
CTCCATTATGCTTGAACCAA+GGG + chr5_2:70772254-70772273 None:intergenic 40.0%
TCTCCATTATGCTTGAACCA+AGG + chr5_2:70772255-70772274 None:intergenic 40.0%
! ACAGGATTTGGCTCATCATG+TGG - chr5_2:70771067-70771086 Msa0785660:CDS 45.0%
CACTAGCACACTTTCTCCTT+AGG + chr5_2:70771105-70771124 None:intergenic 45.0%
GCCTGTTCATAGGAGAAGTA+TGG + chr5_2:70771142-70771161 None:intergenic 45.0%
!! CAATCGCAATTTTGACCGCA+TGG + chr5_2:70771437-70771456 None:intergenic 45.0%
GCTCATCCAAATATGCACCA+AGG + chr5_2:70771604-70771623 None:intergenic 45.0%
CCAGAAAGTTGGAGTAATGG+AGG + chr5_2:70771766-70771785 None:intergenic 45.0%
CCTCCATTACTCCAACTTTC+TGG - chr5_2:70771763-70771782 Msa0785660:intron 45.0%
ATGGCCGTGTAGAGAATTTG+AGG + chr5_2:70771898-70771917 None:intergenic 45.0%
CATTATGCTTGAACCAAGGG+TGG + chr5_2:70772251-70772270 None:intergenic 45.0%
CACCCTTGGTTCAAGCATAA+TGG - chr5_2:70772249-70772268 Msa0785660:CDS 45.0%
! ACAGGATTTGGCTCATCATG+TGG - chr5_2:70771067-70771086 Msa0785660:CDS 45.0%
CACTAGCACACTTTCTCCTT+AGG + chr5_2:70771105-70771124 None:intergenic 45.0%
GCCTGTTCATAGGAGAAGTA+TGG + chr5_2:70771142-70771161 None:intergenic 45.0%
!! CAATCGCAATTTTGACCGCA+TGG + chr5_2:70771437-70771456 None:intergenic 45.0%
GCTCATCCAAATATGCACCA+AGG + chr5_2:70771604-70771623 None:intergenic 45.0%
CCAGAAAGTTGGAGTAATGG+AGG + chr5_2:70771766-70771785 None:intergenic 45.0%
CCTCCATTACTCCAACTTTC+TGG - chr5_2:70771763-70771782 Msa0785660:intron 45.0%
ATGGCCGTGTAGAGAATTTG+AGG + chr5_2:70771898-70771917 None:intergenic 45.0%
CATTATGCTTGAACCAAGGG+TGG + chr5_2:70772251-70772270 None:intergenic 45.0%
CACCCTTGGTTCAAGCATAA+TGG - chr5_2:70772249-70772268 Msa0785660:CDS 45.0%
GTGGAGCATGCAAGTTCCTA+AGG - chr5_2:70771086-70771105 Msa0785660:CDS 50.0%
GCGGTAAAATATGGCCCATG+CGG - chr5_2:70771419-70771438 Msa0785660:intron 50.0%
TGGTTGTGACCTCACAACCA+AGG - chr5_2:70771524-70771543 Msa0785660:intron 50.0%
GTGGAGCATGCAAGTTCCTA+AGG - chr5_2:70771086-70771105 Msa0785660:CDS 50.0%
GCGGTAAAATATGGCCCATG+CGG - chr5_2:70771419-70771438 Msa0785660:intron 50.0%
TGGTTGTGACCTCACAACCA+AGG - chr5_2:70771524-70771543 Msa0785660:intron 50.0%
AGGATCTTGCATGCGAGCCA+AGG + chr5_2:70771281-70771300 None:intergenic 55.0%
TGTGGCCACAGTTGCAGTTG+CGG - chr5_2:70771484-70771503 Msa0785660:intron 55.0%
! GCAGGTTGCTAGCTTGCAAG+AGG - chr5_2:70771981-70772000 Msa0785660:intron 55.0%
AGGGCTTCTTGAACCACCCT+TGG - chr5_2:70772235-70772254 Msa0785660:CDS 55.0%
AGGATCTTGCATGCGAGCCA+AGG + chr5_2:70771281-70771300 None:intergenic 55.0%
TGTGGCCACAGTTGCAGTTG+CGG - chr5_2:70771484-70771503 Msa0785660:intron 55.0%
! GCAGGTTGCTAGCTTGCAAG+AGG - chr5_2:70771981-70772000 Msa0785660:intron 55.0%
AGGGCTTCTTGAACCACCCT+TGG - chr5_2:70772235-70772254 Msa0785660:CDS 55.0%
GTGGTCCGCAACTGCAACTG+TGG + chr5_2:70771492-70771511 None:intergenic 60.0%
GTGGTCCGCAACTGCAACTG+TGG + chr5_2:70771492-70771511 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 70771064 70772287 70771064 ID=Msa0785660;Name=Msa0785660
chr5_2 mRNA 70771064 70772287 70771064 ID=Msa0785660-mRNA-1;Parent=Msa0785660;Name=Msa0785660-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|2|0|193
chr5_2 exon 70772009 70772287 70772009 ID=Msa0785660-mRNA-1:exon:21331;Parent=Msa0785660-mRNA-1
chr5_2 exon 70771064 70771366 70771064 ID=Msa0785660-mRNA-1:exon:21330;Parent=Msa0785660-mRNA-1
chr5_2 CDS 70772009 70772287 70772009 ID=Msa0785660-mRNA-1:cds;Parent=Msa0785660-mRNA-1
chr5_2 CDS 70771064 70771366 70771064 ID=Msa0785660-mRNA-1:cds;Parent=Msa0785660-mRNA-1
Gene Sequence

>Msa0785660

ATGACAGGATTTGGCTCATCATGTGGAGCATGCAAGTTCCTAAGGAGAAAGTGTGCTAGTGATTGTGTTTTTGCTCCATACTTCTCCTATGAACAGGCTTCAACTCATTTTTCAGCAGTGCATAAGGTTTATGGGGCTAGTAATGTCTCTAGACTATTGTCACATCTTCCTATTCAAAATAGAAGTGATGCTGCCATCACTATATCTTATGAAGCCTTGGCTCGCATGCAAGATCCTATTTATGGTTGTGTTGCTCATATCTATGCATTGCAACAACAGGTTGCTAGCTTGCAAGAGGAGATAGATATTCTTGGAAGTGTAATGGCAAATTCAAGTGTTAGTGTTGTGAATTGTGGCAATGTTCAAGCACCAATGAACTCAAATAATGGAACACAATATTACCATAATCAAAGTTTTGGATCATACTTGGATATGGAGCTAATTCCTAATGCACAAGGATTTCCAGAGCCTTTATATGGTGATTCCAACTCAAATACATTAGAGAAGTTCTTATCAGGAATTGATCAAGAGGGCTTCTTGAACCACCCTTGGTTCAAGCATAATGGAGACATGAAGAACTAG
Protein sequence

>Msa0785660

MTGFGSSCGACKFLRRKCASDCVFAPYFSYEQASTHFSAVHKVYGASNVSRLLSHLPIQNRSDAAITISYEALARMQDPIYGCVAHIYALQQQVASLQEEIDILGSVMANSSVSVVNCGNVQAPMNSNNGTQYYHNQSFGSYLDMELIPNAQGFPEPLYGDSNSNTLEKFLSGIDQEGFLNHPWFKHNGDMKN*