AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0787820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787820 MtrunA17_Chr5g0445971 88.095 168 17 2 1 168 1 165 4.03e-105 301
Msa0787820 MtrunA17_Chr5g0435401 67.857 224 67 3 34 253 44 266 8.76e-98 291
Msa0787820 MtrunA17_Chr5g0435401 66.250 80 26 1 177 255 264 343 4.72e-24 99.8
Msa0787820 MtrunA17_Chr1g0192841 60.606 99 30 2 166 255 44 142 1.85e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787820 AT1G55650.1 24.481 241 144 7 34 245 38 269 2.15e-11 63.5

Find 72 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAAACTGCTTTGTTTATA+TGG 0.157345 5_2:+73887899 Msa0787820:three_prime_UTR
ATTCCTTCTTGAGGAATATT+TGG 0.163911 5_2:-73887344 None:intergenic
ACATAAGAGTGACACGTATT+TGG 0.207652 5_2:+73885181 Msa0787820:five_prime_UTR
AGTAAAACAGGTACAAGTTT+AGG 0.215844 5_2:-73885734 None:intergenic
CTTTCTAAGGTCCAGTTAAA+AGG 0.247346 5_2:+73886533 Msa0787820:intron
CATAAGAGTGACACGTATTT+GGG 0.310690 5_2:+73885182 Msa0787820:five_prime_UTR
TTGAGTTGTCTTGTTGTTAG+AGG 0.323549 5_2:-73885269 None:intergenic
AATTTGACTAGAGTAGTTCT+TGG 0.346592 5_2:-73886626 None:intergenic
TATGAAAGTCCATACAAATA+AGG 0.353156 5_2:+73885226 Msa0787820:five_prime_UTR
GTAAAACAGGTACAAGTTTA+GGG 0.365515 5_2:-73885733 None:intergenic
AGTTGTTTCTGCTCTAAAAC+TGG 0.365684 5_2:+73886289 Msa0787820:CDS
TTCTTGCTTTCCTCTACATA+TGG 0.365771 5_2:-73887578 None:intergenic
AACTACTCTAGTCAAATTAC+AGG 0.369281 5_2:+73886631 Msa0787820:CDS
TGGTAACCACTTTGGTTCCT+TGG 0.370453 5_2:-73887324 None:intergenic
GATGTCGGACATTGATAAAA+AGG 0.374210 5_2:+73887437 Msa0787820:CDS
AGGTTGGCCATGAAAAGAAA+TGG 0.384383 5_2:+73886261 Msa0787820:intron
CAACAATGCAGCAGTGGACT+TGG 0.400057 5_2:+73887747 Msa0787820:three_prime_UTR
TTGAGAAATTGTACTTCTAC+AGG 0.406380 5_2:+73886375 Msa0787820:CDS
AGAGGAAAGCAAGAAAATAA+AGG 0.412073 5_2:+73887586 Msa0787820:CDS
TAGAAGGTCTCCAAGTCATT+AGG 0.425705 5_2:-73885378 None:intergenic
TTGTGACCTCCAAGTAAAAC+AGG 0.431064 5_2:-73885746 None:intergenic
ACATTGGTGAGTTGTGTGTT+TGG 0.432104 5_2:-73885324 None:intergenic
GGAATATTTGGTAACCACTT+TGG 0.442677 5_2:-73887332 None:intergenic
CTCACCAATGTTGTCGTGTC+CGG 0.446758 5_2:+73885336 Msa0787820:CDS
AGTGCACTCAAGATCTTAAG+AGG 0.447998 5_2:+73887650 Msa0787820:three_prime_UTR
GGTTGGCCATGAAAAGAAAT+GGG 0.467471 5_2:+73886262 Msa0787820:intron
GGGTAGCTGTCAAATATTCA+TGG 0.471612 5_2:+73885203 Msa0787820:five_prime_UTR
TTGGATATCTTTGCCGCCAT+CGG 0.472444 5_2:-73886607 None:intergenic
TCGAGTGAGTTTGATATAGA+AGG 0.472599 5_2:-73885394 None:intergenic
CCTGCAACCGCCAATGCAAA+AGG 0.477405 5_2:+73886404 Msa0787820:CDS
TCGACTGAAAGCTGATCATC+AGG 0.477735 5_2:+73887371 Msa0787820:CDS
ATAAACAAAGCAGTTTAGCT+TGG 0.493707 5_2:-73887895 None:intergenic
ACAAGTTTAGGGATGTTTCT+CGG 0.498193 5_2:-73885722 None:intergenic
GTACGGTAGATTTGCTAACC+TGG 0.501073 5_2:+73887797 Msa0787820:three_prime_UTR
AACAAATGGCTATTGATACT+TGG 0.501313 5_2:+73887409 Msa0787820:CDS
CATACGCGTCCGAAAACAAA+TGG 0.508342 5_2:+73887395 Msa0787820:CDS
GTCGAGCACATTCCTTCTTG+AGG 0.518278 5_2:-73887353 None:intergenic
TGAATGACTGTACTAGTTGT+TGG 0.526633 5_2:+73887825 Msa0787820:three_prime_UTR
TACTTGGAGGTCACAAGAAG+AGG 0.530713 5_2:+73885753 Msa0787820:CDS
GTATTCTTCTCCTAATGACT+TGG 0.531995 5_2:+73885368 Msa0787820:CDS
GTACAGTCATTCATATTCCC+AGG 0.539258 5_2:-73887815 None:intergenic
GAATGACTGTACTAGTTGTT+GGG 0.543555 5_2:+73887826 Msa0787820:three_prime_UTR
AATGTTGAATGTTTATCGTA+CGG 0.547849 5_2:+73887780 Msa0787820:three_prime_UTR
AAGGTCCAGTTAAAAGGAAG+CGG 0.550807 5_2:+73886539 Msa0787820:intron
CTAACAACAAGACAACTCAA+TGG 0.551952 5_2:+73885272 Msa0787820:exon
CCAATGTTGTCGTGTCCGGC+GGG 0.557640 5_2:+73885340 Msa0787820:CDS
ACCAATGTTGTCGTGTCCGG+CGG 0.561143 5_2:+73885339 Msa0787820:CDS
CTCAATGGAAGACAAGGAAA+CGG 0.569924 5_2:+73885287 Msa0787820:exon
AGGTCCAGTTAAAAGGAAGC+GGG 0.572045 5_2:+73886540 Msa0787820:intron
CCGTCGATTGAGATAAACTA+GGG 0.572781 5_2:-73886570 None:intergenic
GTTTCTGCTCTAAAACTGGA+AGG 0.575106 5_2:+73886293 Msa0787820:CDS
TTACCAAATATTCCTCAAGA+AGG 0.579179 5_2:+73887341 Msa0787820:CDS
CCCGCCGGACACGACAACAT+TGG 0.584078 5_2:-73885340 None:intergenic
GGACGATGAAGATTATCCGA+TGG 0.584176 5_2:+73886591 Msa0787820:CDS
CCCTAGTTTATCTCAATCGA+CGG 0.593570 5_2:+73886570 Msa0787820:CDS
TTTGCATTGGCGGTTGCAGG+AGG 0.593878 5_2:-73886401 None:intergenic
TCCGTCGATTGAGATAAACT+AGG 0.600008 5_2:-73886571 None:intergenic
AGACAACTCAATGGAAGACA+AGG 0.602364 5_2:+73885281 Msa0787820:exon
TTGGAGGTCACAAGAAGAGG+AGG 0.603112 5_2:+73885756 Msa0787820:CDS
TACGGTAGATTTGCTAACCT+GGG 0.603269 5_2:+73887798 Msa0787820:three_prime_UTR
AGAAACAGAGAGGTGCTCCA+AGG 0.610360 5_2:+73887307 Msa0787820:CDS
TTTCATGTAGCCATATGTAG+AGG 0.614803 5_2:+73887568 Msa0787820:intron
TGATACTTGGAAGAAGATGT+CGG 0.619673 5_2:+73887422 Msa0787820:CDS
CAAGGAAACGGTGAAGACGA+CGG 0.643465 5_2:+73885299 Msa0787820:CDS
GTGCACTCAAGATCTTAAGA+GGG 0.655456 5_2:+73887651 Msa0787820:three_prime_UTR
CGATGAAGATTATCCGATGG+CGG 0.681671 5_2:+73886594 Msa0787820:CDS
AGAAGAATACTGTTTCCCGC+CGG 0.682409 5_2:-73885355 None:intergenic
GAAAAGAAGCATAATGTGTG+TGG 0.683498 5_2:+73887676 Msa0787820:three_prime_UTR
ATAAGAGTGACACGTATTTG+GGG 0.686319 5_2:+73885183 Msa0787820:five_prime_UTR
AAAGAAAAGAAGAAACAGAG+AGG 0.700139 5_2:+73887297 Msa0787820:CDS
GGTGCTCCAAGGAACCAAAG+TGG 0.716106 5_2:+73887318 Msa0787820:CDS
GTTGGCCATGAAAAGAAATG+GGG 0.719191 5_2:+73886263 Msa0787820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTATTACATATAATTATT+TGG + chr5_2:73885638-73885657 Msa0787820:intron 10.0%
!! GTTATTACATATAATTATTT+GGG + chr5_2:73885639-73885658 Msa0787820:intron 10.0%
!!! TTATTTTTTTAGAAATGAAG+AGG + chr5_2:73885846-73885865 Msa0787820:intron 15.0%
!! ATTAAAAGTTGAAAGATTTA+TGG + chr5_2:73887492-73887511 Msa0787820:intron 15.0%
!!! TCTGAGGAATTTTTTTTATA+AGG - chr5_2:73886193-73886212 None:intergenic 20.0%
!!! TTCAATTTACAACGTTTTTA+TGG + chr5_2:73886217-73886236 Msa0787820:intron 20.0%
!! ATTTACTAAAGTTCCAAAAT+CGG + chr5_2:73886847-73886866 Msa0787820:intron 20.0%
!! ATTATTTGGATGAGTTTAAA+GGG + chr5_2:73886933-73886952 Msa0787820:intron 20.0%
!! ATTTCAAATAAACCAACTAT+AGG - chr5_2:73887530-73887549 None:intergenic 20.0%
! TATGAAAGTCCATACAAATA+AGG + chr5_2:73885226-73885245 Msa0787820:five_prime_UTR 25.0%
!! TAGAAAAAGACTCTCTAAAA+CGG - chr5_2:73885554-73885573 None:intergenic 25.0%
! TTGAAACTGCAAAATAGAAT+TGG - chr5_2:73885699-73885718 None:intergenic 25.0%
! ACATCATATATATGATGACA+TGG + chr5_2:73885804-73885823 Msa0787820:intron 25.0%
!!! TTTTTTTAGAAATGAAGAGG+AGG + chr5_2:73885849-73885868 Msa0787820:intron 25.0%
! ACATCATATATATGATGACA+TGG + chr5_2:73885804-73885823 Msa0787820:intron 25.0%
! TCTTGACTAATTTAGATTAC+AGG - chr5_2:73886142-73886161 None:intergenic 25.0%
! TGTAATCTAAATTAGTCAAG+AGG + chr5_2:73886141-73886160 Msa0787820:intron 25.0%
! GTAATCTAAATTAGTCAAGA+GGG + chr5_2:73886142-73886161 Msa0787820:intron 25.0%
! TAATCTAAATTAGTCAAGAG+GGG + chr5_2:73886143-73886162 Msa0787820:intron 25.0%
! TGCTTAAGATAACCATAAAT+TGG - chr5_2:73886443-73886462 None:intergenic 25.0%
! TAGTCAAATTACAGGTAATA+AGG + chr5_2:73886639-73886658 Msa0787820:intron 25.0%
!! GGATCTAGAATATTAATGAT+TGG + chr5_2:73886660-73886679 Msa0787820:intron 25.0%
! GGAAAATTGAATTCATTGAT+TGG + chr5_2:73886681-73886700 Msa0787820:intron 25.0%
!! TTGGTAACTCAATGATTATT+TGG + chr5_2:73886919-73886938 Msa0787820:intron 25.0%
! GATTATTTGGATGAGTTTAA+AGG + chr5_2:73886932-73886951 Msa0787820:intron 25.0%
!!! TCAAAATCTACTTTGAAATC+TGG - chr5_2:73887038-73887057 None:intergenic 25.0%
! TAAAATACGAGCTTGAAATA+TGG + chr5_2:73887076-73887095 Msa0787820:intron 25.0%
!!! TTCTTTTCTTTGTTACTTGA+GGG - chr5_2:73887288-73887307 None:intergenic 25.0%
!!! TATCAATAGCCATTTGTTTT+CGG - chr5_2:73887407-73887426 None:intergenic 25.0%
! AATGTTGAATGTTTATCGTA+CGG + chr5_2:73887780-73887799 Msa0787820:three_prime_UTR 25.0%
!!! AAGTCTTTTTTTGGTTTTTG+AGG + chr5_2:73887850-73887869 Msa0787820:three_prime_UTR 25.0%
!! AATGTTTTCACATTTCACTA+GGG + chr5_2:73887937-73887956 Msa0787820:three_prime_UTR 25.0%
!! GAGCATTTTCCTTATTTGTA+TGG - chr5_2:73885238-73885257 None:intergenic 30.0%
GTAAAACAGGTACAAGTTTA+GGG - chr5_2:73885736-73885755 None:intergenic 30.0%
AGTAAAACAGGTACAAGTTT+AGG - chr5_2:73885737-73885756 None:intergenic 30.0%
! AAACTTGTACCTGTTTTACT+TGG + chr5_2:73885737-73885756 Msa0787820:CDS 30.0%
! TTTTCACCAGGTATATTGTT+TGG + chr5_2:73885779-73885798 Msa0787820:intron 30.0%
ATGTAACCAAACAATATACC+TGG - chr5_2:73885788-73885807 None:intergenic 30.0%
! TTTTCACCAGGTATATTGTT+TGG + chr5_2:73885779-73885798 Msa0787820:intron 30.0%
ATGTAACCAAACAATATACC+TGG - chr5_2:73885788-73885807 None:intergenic 30.0%
!!! TAGAAGCTACATTTTGGTTA+TGG - chr5_2:73886086-73886105 None:intergenic 30.0%
! TGCAAATAGAAGCTACATTT+TGG - chr5_2:73886092-73886111 None:intergenic 30.0%
TTACAGGCACAACATATTTA+TGG - chr5_2:73886126-73886145 None:intergenic 30.0%
TTGTAAATTGAACATCTCTG+AGG - chr5_2:73886209-73886228 None:intergenic 30.0%
!! TTTTTCAACTTGAGCACATA+AGG - chr5_2:73886330-73886349 None:intergenic 30.0%
TTGAGAAATTGTACTTCTAC+AGG + chr5_2:73886375-73886394 Msa0787820:CDS 30.0%
! AAAAGGTTAACCTTTTGCAT+TGG - chr5_2:73886417-73886436 None:intergenic 30.0%
! ATGCAAAAGGTTAACCTTTT+AGG + chr5_2:73886417-73886436 Msa0787820:intron 30.0%
! TAACCTTTTAGGCCAATTTA+TGG + chr5_2:73886428-73886447 Msa0787820:intron 30.0%
! TAACCATAAATTGGCCTAAA+AGG - chr5_2:73886434-73886453 None:intergenic 30.0%
!!! TGTTTCAGTTTGTTGCATTT+TGG + chr5_2:73886472-73886491 Msa0787820:intron 30.0%
ACCTTAAATACTCCTTTCTA+AGG + chr5_2:73886520-73886539 Msa0787820:intron 30.0%
!! ACCTTAGAAAGGAGTATTTA+AGG - chr5_2:73886524-73886543 None:intergenic 30.0%
AATTTGACTAGAGTAGTTCT+TGG - chr5_2:73886629-73886648 None:intergenic 30.0%
AACTACTCTAGTCAAATTAC+AGG + chr5_2:73886631-73886650 Msa0787820:CDS 30.0%
!! AAAAGTAGCAAAACCGATTT+TGG - chr5_2:73886863-73886882 None:intergenic 30.0%
ATTGGATTCTTACTTAAAGC+AGG + chr5_2:73887225-73887244 Msa0787820:intron 30.0%
!! CTTCTTTTCTTTGTTACTTG+AGG - chr5_2:73887289-73887308 None:intergenic 30.0%
AAAGAAAAGAAGAAACAGAG+AGG + chr5_2:73887297-73887316 Msa0787820:CDS 30.0%
TTACCAAATATTCCTCAAGA+AGG + chr5_2:73887341-73887360 Msa0787820:CDS 30.0%
ATTCCTTCTTGAGGAATATT+TGG - chr5_2:73887347-73887366 None:intergenic 30.0%
AACAAATGGCTATTGATACT+TGG + chr5_2:73887409-73887428 Msa0787820:CDS 30.0%
!! TTTTGCAATTAGCCTATAGT+TGG + chr5_2:73887515-73887534 Msa0787820:intron 30.0%
AGAGGAAAGCAAGAAAATAA+AGG + chr5_2:73887586-73887605 Msa0787820:CDS 30.0%
ATAAACAAAGCAGTTTAGCT+TGG - chr5_2:73887898-73887917 None:intergenic 30.0%
!! GCTAAACTGCTTTGTTTATA+TGG + chr5_2:73887899-73887918 Msa0787820:three_prime_UTR 30.0%
! CAATGTTTTCACATTTCACT+AGG + chr5_2:73887936-73887955 Msa0787820:three_prime_UTR 30.0%
ACATAAGAGTGACACGTATT+TGG + chr5_2:73885181-73885200 Msa0787820:five_prime_UTR 35.0%
CATAAGAGTGACACGTATTT+GGG + chr5_2:73885182-73885201 Msa0787820:five_prime_UTR 35.0%
ATAAGAGTGACACGTATTTG+GGG + chr5_2:73885183-73885202 Msa0787820:five_prime_UTR 35.0%
!! TTGAGTTGTCTTGTTGTTAG+AGG - chr5_2:73885272-73885291 None:intergenic 35.0%
CTAACAACAAGACAACTCAA+TGG + chr5_2:73885272-73885291 Msa0787820:exon 35.0%
GTATTCTTCTCCTAATGACT+TGG + chr5_2:73885368-73885387 Msa0787820:CDS 35.0%
TCGAGTGAGTTTGATATAGA+AGG - chr5_2:73885397-73885416 None:intergenic 35.0%
! TTTTAGACTTCGCTGGAAAA+CGG + chr5_2:73885418-73885437 Msa0787820:CDS 35.0%
!! TGTGTGTTTACTGGTTTGTT+TGG + chr5_2:73885476-73885495 Msa0787820:intron 35.0%
GATGTGAATGATGTGTTTAC+TGG + chr5_2:73885527-73885546 Msa0787820:intron 35.0%
GCAAAATAGAATTGGAACAG+AGG - chr5_2:73885691-73885710 None:intergenic 35.0%
ACAAGTTTAGGGATGTTTCT+CGG - chr5_2:73885725-73885744 None:intergenic 35.0%
GCAATGTCGAAAACTATATG+TGG - chr5_2:73886057-73886076 None:intergenic 35.0%
TTCTCTTGAATGTTTCCTAC+AGG + chr5_2:73886241-73886260 Msa0787820:intron 35.0%
!! AGTTGTTTCTGCTCTAAAAC+TGG + chr5_2:73886289-73886308 Msa0787820:CDS 35.0%
!! GCTCAAGTTGAAAAACTCTA+TGG + chr5_2:73886335-73886354 Msa0787820:CDS 35.0%
!! CTTTTAACTGGACCTTAGAA+AGG - chr5_2:73886535-73886554 None:intergenic 35.0%
!! CTTTCTAAGGTCCAGTTAAA+AGG + chr5_2:73886533-73886552 Msa0787820:intron 35.0%
CACAACTGATCAATTCACTA+TGG + chr5_2:73886817-73886836 Msa0787820:intron 35.0%
! CTGTGGACATGAGTTTATTT+TGG + chr5_2:73886900-73886919 Msa0787820:intron 35.0%
TGGATGAGTTTAAAGGGATT+TGG + chr5_2:73886939-73886958 Msa0787820:intron 35.0%
ACAACCCAAATTCAGTTTAG+AGG + chr5_2:73887164-73887183 Msa0787820:intron 35.0%
TTCTTACTTAAAGCAGGAGA+TGG + chr5_2:73887231-73887250 Msa0787820:intron 35.0%
! GGAATATTTGGTAACCACTT+TGG - chr5_2:73887335-73887354 None:intergenic 35.0%
TGATACTTGGAAGAAGATGT+CGG + chr5_2:73887422-73887441 Msa0787820:CDS 35.0%
GATGTCGGACATTGATAAAA+AGG + chr5_2:73887437-73887456 Msa0787820:CDS 35.0%
TTTCATGTAGCCATATGTAG+AGG + chr5_2:73887568-73887587 Msa0787820:intron 35.0%
TTCTTGCTTTCCTCTACATA+TGG - chr5_2:73887581-73887600 None:intergenic 35.0%
! GAAAAGAAGCATAATGTGTG+TGG + chr5_2:73887676-73887695 Msa0787820:three_prime_UTR 35.0%
TGAATGACTGTACTAGTTGT+TGG + chr5_2:73887825-73887844 Msa0787820:three_prime_UTR 35.0%
GAATGACTGTACTAGTTGTT+GGG + chr5_2:73887826-73887845 Msa0787820:three_prime_UTR 35.0%
!!! TTGTTGGGCAAGTCTTTTTT+TGG + chr5_2:73887841-73887860 Msa0787820:three_prime_UTR 35.0%
GGGTAGCTGTCAAATATTCA+TGG + chr5_2:73885203-73885222 Msa0787820:five_prime_UTR 40.0%
AGACAACTCAATGGAAGACA+AGG + chr5_2:73885281-73885300 Msa0787820:exon 40.0%
CTCAATGGAAGACAAGGAAA+CGG + chr5_2:73885287-73885306 Msa0787820:exon 40.0%
ACATTGGTGAGTTGTGTGTT+TGG - chr5_2:73885327-73885346 None:intergenic 40.0%
TAGAAGGTCTCCAAGTCATT+AGG - chr5_2:73885381-73885400 None:intergenic 40.0%
CCAGTAAACACACATGCAAA+CGG - chr5_2:73885470-73885489 None:intergenic 40.0%
! CTTGTACCTGTTTTACTTGG+AGG + chr5_2:73885740-73885759 Msa0787820:CDS 40.0%
TTGTGACCTCCAAGTAAAAC+AGG - chr5_2:73885749-73885768 None:intergenic 40.0%
GTGGACATACATTCGAACTT+TGG - chr5_2:73886038-73886057 None:intergenic 40.0%
CTTGAATGTTTCCTACAGGT+TGG + chr5_2:73886245-73886264 Msa0787820:intron 40.0%
! AGGTTGGCCATGAAAAGAAA+TGG + chr5_2:73886261-73886280 Msa0787820:intron 40.0%
! GGTTGGCCATGAAAAGAAAT+GGG + chr5_2:73886262-73886281 Msa0787820:intron 40.0%
GTTGGCCATGAAAAGAAATG+GGG + chr5_2:73886263-73886282 Msa0787820:CDS 40.0%
! CTTCACCCCATTTCTTTTCA+TGG - chr5_2:73886271-73886290 None:intergenic 40.0%
!! GTTTCTGCTCTAAAACTGGA+AGG + chr5_2:73886293-73886312 Msa0787820:CDS 40.0%
! AGGTTAACCTTTTGCATTGG+CGG - chr5_2:73886414-73886433 None:intergenic 40.0%
AAGGTCCAGTTAAAAGGAAG+CGG + chr5_2:73886539-73886558 Msa0787820:intron 40.0%
! CCGTCGATTGAGATAAACTA+GGG - chr5_2:73886573-73886592 None:intergenic 40.0%
CCCTAGTTTATCTCAATCGA+CGG + chr5_2:73886570-73886589 Msa0787820:CDS 40.0%
! TCCGTCGATTGAGATAAACT+AGG - chr5_2:73886574-73886593 None:intergenic 40.0%
TGTGTTTATTAGCTGCACTG+TGG + chr5_2:73886883-73886902 Msa0787820:intron 40.0%
TACGAGCTTGAAATATGGAC+CGG + chr5_2:73887081-73887100 Msa0787820:intron 40.0%
GCTGCCTCTAAACTGAATTT+GGG - chr5_2:73887171-73887190 None:intergenic 40.0%
AGCTGCCTCTAAACTGAATT+TGG - chr5_2:73887172-73887191 None:intergenic 40.0%
GTGCTTATGCTGCAATACAT+TGG + chr5_2:73887207-73887226 Msa0787820:intron 40.0%
AGTGCACTCAAGATCTTAAG+AGG + chr5_2:73887650-73887669 Msa0787820:three_prime_UTR 40.0%
GTGCACTCAAGATCTTAAGA+GGG + chr5_2:73887651-73887670 Msa0787820:three_prime_UTR 40.0%
! TTTTGTCAACAATGCAGCAG+TGG + chr5_2:73887741-73887760 Msa0787820:three_prime_UTR 40.0%
!! TACGGTAGATTTGCTAACCT+GGG + chr5_2:73887798-73887817 Msa0787820:three_prime_UTR 40.0%
GTACAGTCATTCATATTCCC+AGG - chr5_2:73887818-73887837 None:intergenic 40.0%
AGAAGAATACTGTTTCCCGC+CGG - chr5_2:73885358-73885377 None:intergenic 45.0%
! ACTCGACTTTTAGACTTCGC+TGG + chr5_2:73885411-73885430 Msa0787820:CDS 45.0%
CCGTTTGCATGTGTGTTTAC+TGG + chr5_2:73885467-73885486 Msa0787820:intron 45.0%
TACTTGGAGGTCACAAGAAG+AGG + chr5_2:73885753-73885772 Msa0787820:CDS 45.0%
! AAGAAGAGGAGGTTTTCACC+AGG + chr5_2:73885767-73885786 Msa0787820:CDS 45.0%
! ATGAAGAGGAGGTTTTCACC+AGG + chr5_2:73885860-73885879 Msa0787820:intron 45.0%
AGCCTTCAACTTCAACCTAC+AGG + chr5_2:73885978-73885997 Msa0787820:intron 45.0%
GCCTTCAACTTCAACCTACA+GGG + chr5_2:73885979-73885998 Msa0787820:intron 45.0%
CCTTCAACTTCAACCTACAG+GGG + chr5_2:73885980-73885999 Msa0787820:intron 45.0%
TTGCTAGATTCAACCCCTGT+AGG - chr5_2:73885996-73886015 None:intergenic 45.0%
! TCTTTTCATGGCCAACCTGT+AGG - chr5_2:73886259-73886278 None:intergenic 45.0%
AGGTCCAGTTAAAAGGAAGC+GGG + chr5_2:73886540-73886559 Msa0787820:intron 45.0%
GGACGATGAAGATTATCCGA+TGG + chr5_2:73886591-73886610 Msa0787820:CDS 45.0%
CGATGAAGATTATCCGATGG+CGG + chr5_2:73886594-73886613 Msa0787820:CDS 45.0%
TTGGATATCTTTGCCGCCAT+CGG - chr5_2:73886610-73886629 None:intergenic 45.0%
GGTGGTTCAAACAAGATCTG+CGG + chr5_2:73887011-73887030 Msa0787820:intron 45.0%
AGCACATCGTCGTTCAATCA+AGG - chr5_2:73887111-73887130 None:intergenic 45.0%
! TGGTAACCACTTTGGTTCCT+TGG - chr5_2:73887327-73887346 None:intergenic 45.0%
TCGACTGAAAGCTGATCATC+AGG + chr5_2:73887371-73887390 Msa0787820:CDS 45.0%
CATACGCGTCCGAAAACAAA+TGG + chr5_2:73887395-73887414 Msa0787820:CDS 45.0%
!! GTACGGTAGATTTGCTAACC+TGG + chr5_2:73887797-73887816 Msa0787820:three_prime_UTR 45.0%
CAAGGAAACGGTGAAGACGA+CGG + chr5_2:73885299-73885318 Msa0787820:CDS 50.0%
CTCACCAATGTTGTCGTGTC+CGG + chr5_2:73885336-73885355 Msa0787820:CDS 50.0%
TTGGAGGTCACAAGAAGAGG+AGG + chr5_2:73885756-73885775 Msa0787820:CDS 50.0%
! CCCCTGTAGGTTGAAGTTGA+AGG - chr5_2:73885983-73886002 None:intergenic 50.0%
! GAGTCCCGCTTCCTTTTAAC+TGG - chr5_2:73886547-73886566 None:intergenic 50.0%
TGTTTGAACCACCACCGATG+TGG - chr5_2:73887004-73887023 None:intergenic 50.0%
GTCGTTCAATCAAGGTCGTC+CGG - chr5_2:73887103-73887122 None:intergenic 50.0%
!! AGAAACAGAGAGGTGCTCCA+AGG + chr5_2:73887307-73887326 Msa0787820:CDS 50.0%
GTCGAGCACATTCCTTCTTG+AGG - chr5_2:73887356-73887375 None:intergenic 50.0%
! CAACAATGCAGCAGTGGACT+TGG + chr5_2:73887747-73887766 Msa0787820:three_prime_UTR 50.0%
ACCAATGTTGTCGTGTCCGG+CGG + chr5_2:73885339-73885358 Msa0787820:CDS 55.0%
! TTTGCATTGGCGGTTGCAGG+AGG - chr5_2:73886404-73886423 None:intergenic 55.0%
!! CCTTTTGCATTGGCGGTTGC+AGG - chr5_2:73886407-73886426 None:intergenic 55.0%
CCTGCAACCGCCAATGCAAA+AGG + chr5_2:73886404-73886423 Msa0787820:CDS 55.0%
! CCGATGTGGTTGAAAGCGTG+CGG - chr5_2:73886990-73887009 None:intergenic 55.0%
CCGCACGCTTTCAACCACAT+CGG + chr5_2:73886987-73887006 Msa0787820:intron 55.0%
CACGCTTTCAACCACATCGG+TGG + chr5_2:73886990-73887009 Msa0787820:intron 55.0%
GCTTTCAACCACATCGGTGG+TGG + chr5_2:73886993-73887012 Msa0787820:intron 55.0%
GGTGCTCCAAGGAACCAAAG+TGG + chr5_2:73887318-73887337 Msa0787820:CDS 55.0%
CCAATGTTGTCGTGTCCGGC+GGG + chr5_2:73885340-73885359 Msa0787820:CDS 60.0%
AGCGTGCGGTCACAGGATTG+TGG - chr5_2:73886976-73886995 None:intergenic 60.0%
! GGTTGAAAGCGTGCGGTCAC+AGG - chr5_2:73886983-73887002 None:intergenic 60.0%
CCCGCCGGACACGACAACAT+TGG - chr5_2:73885343-73885362 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 73885162 73887986 73885162 ID=Msa0787820;Name=Msa0787820
chr5_2 mRNA 73885162 73887986 73885162 ID=Msa0787820-mRNA-1;Parent=Msa0787820;Name=Msa0787820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=129|0.8|0.83|1|1|1|6|352|255
chr5_2 exon 73885162 73885439 73885162 ID=Msa0787820-mRNA-1:exon:22484;Parent=Msa0787820-mRNA-1
chr5_2 exon 73885713 73885788 73885713 ID=Msa0787820-mRNA-1:exon:22485;Parent=Msa0787820-mRNA-1
chr5_2 exon 73886263 73886425 73886263 ID=Msa0787820-mRNA-1:exon:22486;Parent=Msa0787820-mRNA-1
chr5_2 exon 73886542 73886652 73886542 ID=Msa0787820-mRNA-1:exon:22487;Parent=Msa0787820-mRNA-1
chr5_2 exon 73887247 73887458 73887247 ID=Msa0787820-mRNA-1:exon:22488;Parent=Msa0787820-mRNA-1
chr5_2 exon 73887578 73887986 73887578 ID=Msa0787820-mRNA-1:exon:22489;Parent=Msa0787820-mRNA-1
chr5_2 five_prime_UTR 73885162 73885290 73885162 ID=Msa0787820-mRNA-1:five_prime_utr;Parent=Msa0787820-mRNA-1
chr5_2 CDS 73885291 73885439 73885291 ID=Msa0787820-mRNA-1:cds;Parent=Msa0787820-mRNA-1
chr5_2 CDS 73885713 73885788 73885713 ID=Msa0787820-mRNA-1:cds;Parent=Msa0787820-mRNA-1
chr5_2 CDS 73886263 73886425 73886263 ID=Msa0787820-mRNA-1:cds;Parent=Msa0787820-mRNA-1
chr5_2 CDS 73886542 73886652 73886542 ID=Msa0787820-mRNA-1:cds;Parent=Msa0787820-mRNA-1
chr5_2 CDS 73887247 73887458 73887247 ID=Msa0787820-mRNA-1:cds;Parent=Msa0787820-mRNA-1
chr5_2 CDS 73887578 73887634 73887578 ID=Msa0787820-mRNA-1:cds;Parent=Msa0787820-mRNA-1
chr5_2 three_prime_UTR 73887635 73887986 73887635 ID=Msa0787820-mRNA-1:three_prime_utr;Parent=Msa0787820-mRNA-1
Gene Sequence

>Msa0787820

ATGGAAGACAAGGAAACGGTGAAGACGACGGTTCCAAACACACAACTCACCAATGTTGTCGTGTCCGGCGGGAAACAGTATTCTTCTCCTAATGACTTGGAGACCTTCTATATCAAACTCACTCGACTTTTAGACTTCGCTGGAAAACGTTTCAATGTCCGAGAAACATCCCTAAACTTGTACCTGTTTTACTTGGAGGTCACAAGAAGAGGAGGTTTTCACCAGGTTGGCCATGAAAAGAAATGGGGTGAAGTTGTTTCTGCTCTAAAACTGGAAGGAAACAATGCAACCTTATGTGCTCAAGTTGAAAAACTCTATGGTCATCTTCTTTACGAATTTGAGAAATTGTACTTCTACAGGTCTCCTCCTGCAACCGCCAATGCAAAAGGTCCAGTTAAAAGGAAGCGGGACTCATGCCCTAGTTTATCTCAATCGACGGACGATGAAGATTATCCGATGGCGGCAAAGATATCCAAGAACTACTCTAGTCAAATTACAGGAGATGGATATCATGAATCTCAAGTGTTTCAACAAGCACCCTCAAGTAACAAAGAAAAGAAGAAACAGAGAGGTGCTCCAAGGAACCAAAGTGGTTACCAAATATTCCTCAAGAAGGAATGTGCTCGACTGAAAGCTGATCATCAGGACATACGCGTCCGAAAACAAATGGCTATTGATACTTGGAAGAAGATGTCGGACATTGATAAAAAGCCATATGTAGAGGAAAGCAAGAAAATAAAGGAAAAAAACAAAGAAGCAATGATATAG
Protein sequence

>Msa0787820

MEDKETVKTTVPNTQLTNVVVSGGKQYSSPNDLETFYIKLTRLLDFAGKRFNVRETSLNLYLFYLEVTRRGGFHQVGHEKKWGEVVSALKLEGNNATLCAQVEKLYGHLLYEFEKLYFYRSPPATANAKGPVKRKRDSCPSLSQSTDDEDYPMAAKISKNYSSQITGDGYHESQVFQQAPSSNKEKKKQRGAPRNQSGYQIFLKKECARLKADHQDIRVRKQMAIDTWKKMSDIDKKPYVEESKKIKEKNKEAMI*