Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0788170 | AET00849.1 | 40.650 | 123 | 58 | 5 | 1 | 109 | 1 | 122 | 4.98e-15 | 75.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0788170 | G7K4S9 | 40.650 | 123 | 58 | 5 | 1 | 109 | 1 | 122 | 2.38e-15 | 75.9 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0788170 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0788170 | MtrunA17_Chr5g0447061 | 40.650 | 123 | 58 | 5 | 1 | 109 | 1 | 122 | 4.58e-19 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGTACTCCTTCTTGCTTC+TGG | 0.258286 | 5_2:-74527711 | None:intergenic |
| ATCAAGGAGGAGTTGGAAAT+TGG | 0.290061 | 5_2:+74527625 | Msa0788170:CDS |
| TTTGTTCCACCACAAAATTA+TGG | 0.333981 | 5_2:+74527764 | Msa0788170:CDS |
| TTTCTTTAGGCTATTACTCA+TGG | 0.421485 | 5_2:+74528059 | Msa0788170:intron |
| CACCACAAAATTATGGAGCT+TGG | 0.430852 | 5_2:+74527771 | Msa0788170:CDS |
| CGTTGGTGCGGTAACGGTCT+CGG | 0.443472 | 5_2:-74528155 | None:intergenic |
| AATCACCAGGGAGCTCTTCA+AGG | 0.457956 | 5_2:-74527733 | None:intergenic |
| CATATCCGTTGGTGCGGTAA+CGG | 0.463180 | 5_2:-74528161 | None:intergenic |
| TTCTTTAGGCTATTACTCAT+GGG | 0.494106 | 5_2:+74528060 | Msa0788170:intron |
| ATGGCAAATCAAGGAGGAGT+TGG | 0.501152 | 5_2:+74527618 | Msa0788170:CDS |
| ACCGCACCAACGGATATGCT+TGG | 0.552860 | 5_2:+74528166 | Msa0788170:CDS |
| CTAGAGAGATTGTGTTGACG+AGG | 0.558612 | 5_2:+74528118 | Msa0788170:CDS |
| ACAAATTGCTCGAAATCACC+AGG | 0.563161 | 5_2:-74527746 | None:intergenic |
| TACTACCTTGAAGAGCTCCC+TGG | 0.577785 | 5_2:+74527728 | Msa0788170:CDS |
| CGAGACCGTTACCGCACCAA+CGG | 0.627996 | 5_2:+74528156 | Msa0788170:CDS |
| TCCAAGCATATCCGTTGGTG+CGG | 0.629727 | 5_2:-74528167 | None:intergenic |
| TTTACGGCCGAAAATGGACG+CGG | 0.649194 | 5_2:+74528091 | Msa0788170:CDS |
| GTTCATCCAAGCATATCCGT+TGG | 0.653087 | 5_2:-74528172 | None:intergenic |
| TGGAGCTTGGAGTAATGATG+AGG | 0.655946 | 5_2:+74527784 | Msa0788170:CDS |
| TTCACAATGGCAAATCAAGG+AGG | 0.686222 | 5_2:+74527612 | None:intergenic |
| CAAATTGCTCGAAATCACCA+GGG | 0.727892 | 5_2:-74527745 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTAATGAAAAATAACA+TGG | - | chr5_2:74527925-74527944 | None:intergenic | 10.0% |
| !!! | AACATATTTTAATCAAGAAA+AGG | - | chr5_2:74527902-74527921 | None:intergenic | 15.0% |
| !!! | TAATGTTTTCTATTTTCTTT+AGG | + | chr5_2:74528046-74528065 | Msa0788170:intron | 15.0% |
| !! | TCATTAAAATATGTGTTCTT+TGG | + | chr5_2:74527831-74527850 | Msa0788170:intron | 20.0% |
| !!! | TCTTTGGTTTTTTTTTTTGA+AGG | + | chr5_2:74527847-74527866 | Msa0788170:intron | 20.0% |
| !!! | AACATGTTTTAATCAAGAAA+AGG | - | chr5_2:74528003-74528022 | None:intergenic | 20.0% |
| ! | TCATTAAAATATGCGTTCTT+TGG | + | chr5_2:74527934-74527953 | Msa0788170:intron | 25.0% |
| ! | AAATTTTTCCAGAAGCAAGA+AGG | + | chr5_2:74527703-74527722 | Msa0788170:CDS | 30.0% |
| TTTGTTCCACCACAAAATTA+TGG | + | chr5_2:74527764-74527783 | Msa0788170:CDS | 30.0% | |
| !!! | GTTCTTTGGTTTTTTGTTGA+AGG | + | chr5_2:74527948-74527967 | Msa0788170:intron | 30.0% |
| TTTCTTTAGGCTATTACTCA+TGG | + | chr5_2:74528059-74528078 | Msa0788170:intron | 30.0% | |
| TTCTTTAGGCTATTACTCAT+GGG | + | chr5_2:74528060-74528079 | Msa0788170:intron | 30.0% | |
| ! | CTCATGGGATTGAGATTTTA+CGG | + | chr5_2:74528075-74528094 | Msa0788170:CDS | 35.0% |
| ATCAAGGAGGAGTTGGAAAT+TGG | + | chr5_2:74527625-74527644 | Msa0788170:CDS | 40.0% | |
| CAAATTGCTCGAAATCACCA+GGG | - | chr5_2:74527748-74527767 | None:intergenic | 40.0% | |
| ACAAATTGCTCGAAATCACC+AGG | - | chr5_2:74527749-74527768 | None:intergenic | 40.0% | |
| ! | CAAGCTCCATAATTTTGTGG+TGG | - | chr5_2:74527773-74527792 | None:intergenic | 40.0% |
| CACCACAAAATTATGGAGCT+TGG | + | chr5_2:74527771-74527790 | Msa0788170:CDS | 40.0% | |
| ! | CTCCAAGCTCCATAATTTTG+TGG | - | chr5_2:74527776-74527795 | None:intergenic | 40.0% |
| ! | TGAGATTTTACGGCCGAAAA+TGG | + | chr5_2:74528085-74528104 | Msa0788170:CDS | 40.0% |
| ! | TAGAAATCCGCGTCCATTTT+CGG | - | chr5_2:74528101-74528120 | None:intergenic | 40.0% |
| ATGGCAAATCAAGGAGGAGT+TGG | + | chr5_2:74527618-74527637 | Msa0788170:CDS | 45.0% | |
| GTAGTACTCCTTCTTGCTTC+TGG | - | chr5_2:74527714-74527733 | None:intergenic | 45.0% | |
| TGGAGCTTGGAGTAATGATG+AGG | + | chr5_2:74527784-74527803 | Msa0788170:CDS | 45.0% | |
| ! | CTAGAGAGATTGTGTTGACG+AGG | + | chr5_2:74528118-74528137 | Msa0788170:CDS | 45.0% |
| GTTCATCCAAGCATATCCGT+TGG | - | chr5_2:74528175-74528194 | None:intergenic | 45.0% | |
| TACTACCTTGAAGAGCTCCC+TGG | + | chr5_2:74527728-74527747 | Msa0788170:CDS | 50.0% | |
| AATCACCAGGGAGCTCTTCA+AGG | - | chr5_2:74527736-74527755 | None:intergenic | 50.0% | |
| TTTACGGCCGAAAATGGACG+CGG | + | chr5_2:74528091-74528110 | Msa0788170:CDS | 50.0% | |
| CATATCCGTTGGTGCGGTAA+CGG | - | chr5_2:74528164-74528183 | None:intergenic | 50.0% | |
| TCCAAGCATATCCGTTGGTG+CGG | - | chr5_2:74528170-74528189 | None:intergenic | 50.0% | |
| ACCGCACCAACGGATATGCT+TGG | + | chr5_2:74528166-74528185 | Msa0788170:CDS | 55.0% | |
| ! | CGTTGGTGCGGTAACGGTCT+CGG | - | chr5_2:74528158-74528177 | None:intergenic | 60.0% |
| CGAGACCGTTACCGCACCAA+CGG | + | chr5_2:74528156-74528175 | Msa0788170:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 74527618 | 74528200 | 74527618 | ID=Msa0788170;Name=Msa0788170 |
| chr5_2 | mRNA | 74527618 | 74528200 | 74527618 | ID=Msa0788170-mRNA-1;Parent=Msa0788170;Name=Msa0788170-mRNA-1;_AED=0.02;_eAED=0.29;_QI=0|0|0|1|1|1|2|0|109 |
| chr5_2 | exon | 74527618 | 74527814 | 74527618 | ID=Msa0788170-mRNA-1:exon:22754;Parent=Msa0788170-mRNA-1 |
| chr5_2 | exon | 74528068 | 74528200 | 74528068 | ID=Msa0788170-mRNA-1:exon:22755;Parent=Msa0788170-mRNA-1 |
| chr5_2 | CDS | 74527618 | 74527814 | 74527618 | ID=Msa0788170-mRNA-1:cds;Parent=Msa0788170-mRNA-1 |
| chr5_2 | CDS | 74528068 | 74528200 | 74528068 | ID=Msa0788170-mRNA-1:cds;Parent=Msa0788170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
