Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0788630 | sp|A0A2R6S148|MYB1_ACTCC | 78.082 | 73 | 16 | 0 | 6 | 78 | 40 | 112 | 1.70e-35 | 127 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0788630 | MtrunA17_Chr7g0220341 | 61.453 | 179 | 58 | 3 | 6 | 176 | 59 | 234 | 1.47e-71 | 218 |
| Msa0788630 | MtrunA17_Chr5g0434911 | 66.456 | 158 | 48 | 3 | 6 | 163 | 53 | 205 | 7.49e-58 | 182 |
| Msa0788630 | MtrunA17_Chr5g0434931 | 59.116 | 181 | 61 | 5 | 6 | 177 | 67 | 243 | 3.71e-56 | 178 |
| Msa0788630 | MtrunA17_Chr7g0229131 | 57.616 | 151 | 53 | 2 | 3 | 153 | 1 | 140 | 2.63e-52 | 165 |
| Msa0788630 | MtrunA17_Chr7g0228991 | 56.216 | 185 | 66 | 6 | 6 | 179 | 61 | 241 | 1.28e-50 | 164 |
| Msa0788630 | MtrunA17_Chr5g0434831 | 64.336 | 143 | 46 | 2 | 6 | 144 | 39 | 180 | 2.08e-50 | 161 |
| Msa0788630 | MtrunA17_Chr8g0360111 | 56.354 | 181 | 54 | 4 | 6 | 178 | 57 | 220 | 4.75e-50 | 162 |
| Msa0788630 | MtrunA17_Chr5g0434841 | 65.035 | 143 | 45 | 2 | 6 | 144 | 52 | 193 | 1.12e-48 | 160 |
| Msa0788630 | MtrunA17_Chr7g0229101 | 54.396 | 182 | 61 | 3 | 3 | 175 | 1 | 169 | 2.94e-48 | 156 |
| Msa0788630 | MtrunA17_Chr5g0434901 | 76.991 | 113 | 24 | 2 | 8 | 120 | 7 | 117 | 1.92e-46 | 148 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0788630 | AT5G35550.2 | 59.341 | 91 | 37 | 0 | 1 | 91 | 44 | 134 | 1.30e-33 | 120 |
| Msa0788630 | AT5G14750.1 | 63.415 | 82 | 30 | 0 | 6 | 87 | 48 | 129 | 3.48e-33 | 117 |
| Msa0788630 | AT1G66370.1 | 73.611 | 72 | 19 | 0 | 6 | 77 | 40 | 111 | 5.06e-33 | 118 |
| Msa0788630 | AT5G40330.1 | 69.333 | 75 | 23 | 0 | 6 | 80 | 44 | 118 | 8.78e-33 | 117 |
| Msa0788630 | AT3G13540.1 | 69.863 | 73 | 22 | 0 | 6 | 78 | 55 | 127 | 2.79e-32 | 116 |
| Msa0788630 | AT5G35550.1 | 61.628 | 86 | 33 | 0 | 6 | 91 | 46 | 131 | 3.08e-32 | 117 |
| Msa0788630 | AT5G14750.3 | 64.103 | 78 | 28 | 0 | 10 | 87 | 18 | 95 | 3.40e-32 | 114 |
| Msa0788630 | AT3G27920.1 | 65.385 | 78 | 27 | 0 | 2 | 79 | 42 | 119 | 5.85e-32 | 115 |
| Msa0788630 | AT4G38620.1 | 62.338 | 77 | 29 | 0 | 2 | 78 | 40 | 116 | 8.66e-30 | 111 |
| Msa0788630 | AT2G16720.1 | 62.338 | 77 | 29 | 0 | 2 | 78 | 40 | 116 | 1.91e-29 | 110 |
Find 32 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTATTTGTTACACTTGGC+AGG | 0.457899 | 5_2:+75769505 | None:intergenic |
| AATGAGGCATTAGAATCTCT+GGG | 0.582303 | 5_2:-75770083 | Msa0788620:intron |
| ACATCATTCGCTGTTCTACC+GGG | 0.568549 | 5_2:-75769846 | Msa0788620:intron |
| ACGGATAATTTGAGGAACTT+GGG | 0.485121 | 5_2:+75770173 | Msa0788630:CDS |
| ACTACCATGTGCAATGTTGA+CGG | 0.489875 | 5_2:+75770154 | Msa0788630:CDS |
| ACTATGGTTTCAAATCAAAT+TGG | 0.342867 | 5_2:+75770110 | Msa0788630:CDS |
| ACTATTGGCACACAAATTTG+CGG | 0.532907 | 5_2:+75769874 | Msa0788630:CDS |
| AGAGATTCTAATGCCTCATT+AGG | 0.396204 | 5_2:+75770086 | Msa0788630:CDS |
| ATAAGGTTACACAATCTTCT+AGG | 0.262250 | 5_2:+75769628 | Msa0788630:CDS |
| ATGAAGCTGATATGATCATA+AGG | 0.505340 | 5_2:+75769611 | Msa0788630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGCTTTCTCTGTTGATATT+GGG | - | chr5_2:75769583-75769602 | Msa0788620:intron | 30.0% | |
| ATGAAGCTGATATGATCATA+AGG | + | chr5_2:75769611-75769630 | Msa0788630:CDS | 30.0% | |
| ATAAGGTTACACAATCTTCT+AGG | + | chr5_2:75769628-75769647 | Msa0788630:CDS | 30.0% | |
| TAAGGTTACACAATCTTCTA+GGG | + | chr5_2:75769629-75769648 | Msa0788630:CDS | 30.0% | |
| CGAATGATGTGAAAAACTAT+TGG | + | chr5_2:75769859-75769878 | Msa0788630:CDS | 30.0% | |
| TTGAAAAAAATTCCAGGTTG+AGG | - | chr5_2:75769983-75770002 | Msa0788620:intron | 30.0% | |
| TCAGAGAAATGAAATCACAA+AGG | - | chr5_2:75770219-75770238 | None:intergenic | 30.0% | |
| GAAAAAGTTGTAGATTGAGG+TGG | + | chr5_2:75769542-75769561 | Msa0788630:CDS | 35.0% | |
| GATGCTTTCTCTGTTGATAT+TGG | - | chr5_2:75769584-75769603 | Msa0788620:intron | 35.0% | |
| ACTATTGGCACACAAATTTG+CGG | + | chr5_2:75769874-75769893 | Msa0788630:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 75769508 | 75770238 | 75769508 | ID=Msa0788630;Name=Msa0788630 |
| chr5_2 | mRNA | 75769508 | 75770238 | 75769508 | ID=Msa0788630-mRNA-1;Parent=Msa0788630;Name=Msa0788630-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|188 |
| chr5_2 | exon | 75769508 | 75769656 | 75769508 | ID=Msa0788630-mRNA-1:exon:23023;Parent=Msa0788630-mRNA-1 |
| chr5_2 | exon | 75769821 | 75770238 | 75769821 | ID=Msa0788630-mRNA-1:exon:23024;Parent=Msa0788630-mRNA-1 |
| chr5_2 | CDS | 75769508 | 75769656 | 75769508 | ID=Msa0788630-mRNA-1:cds;Parent=Msa0788630-mRNA-1 |
| chr5_2 | CDS | 75769821 | 75770238 | 75769821 | ID=Msa0788630-mRNA-1:cds;Parent=Msa0788630-mRNA-1 |
| Gene Sequence |
>Msa0788630 TATTTGTTACACTTGGCAGGATTGAATAGATGCCGAAAAAGTTGTAGATTGAGGTGGTTAAATTATTTAAATCCCAATATCAACAGAGAAAGCATCACCGAGGATGAAGCTGATATGATCATAAGGTTACACAATCTTCTAGGGCATAGATGGTCATTGATTGCTGCAAGGCTTCCCGGTAGAACAGCGAATGATGTGAAAAACTATTGGCACACAAATTTGCGGAAGAAGCTTGCTTTAGAAAAAGAAGAGAAGAAAGAAAAAGAGAAACATAAGGAAACTATGAAAACACACATGAAGTTATTAAGCCTCAACCTGGAATTTTTTTCAACTCATTCACCATGGCTGAATAAGAAGCAAAATAATTTTGTGACACAACCAGTACTGGCTTCAAATAAAGATGGTAATGTTCCCAGAGATTCTAATGCCTCATTAGGTACTATGGTTTCAAATCAAATTGGTAGAGATTGTGCTTCTGCTTCACTACCATGTGCAATGTTGACGGATAATTTGAGGAACTTGGGGGAGTATTTCGGGATTTCAACCTTTGTGATTTCATTTCTCTGA |
| Protein sequence |
>Msa0788630 YLLHLAGLNRCRKSCRLRWLNYLNPNINRESITEDEADMIIRLHNLLGHRWSLIAARLPGRTANDVKNYWHTNLRKKLALEKEEKKEKEKHKETMKTHMKLLSLNLEFFSTHSPWLNKKQNNFVTQPVLASNKDGNVPRDSNASLGTMVSNQIGRDCASASLPCAMLTDNLRNLGEYFGISTFVISFL* |
