AlfalfaGEDB | Msa0789610

M. sativa cultivar zhongmu-4 / Msa0789610

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0789610	sp\|Q9SWG3\|FAR1_ARATH	33.333	249	131	6	39	252	1	249	1.91e-32	128

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0789610	A0A2K3NBU0	59.130	230	70	4	45	252	3	230	1.39e-79	263

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TFs/TRs:

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Gene ID	Type	Classification
Msa0789610	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0789610	MtrunA17_Chr1g0207551	34.158	202	111	4	73	252	28	229	1.35e-28	114
Msa0789610	MtrunA17_Chr2g0329031	34.597	211	103	6	67	252	2	202	1.89e-28	113
Msa0789610	MtrunA17_Chr3g0080351	33.019	212	84	5	77	252	74	263	3.42e-28	112
Msa0789610	MtrunA17_Chr5g0448761	30.542	203	111	6	77	250	58	259	5.87e-21	92.0
Msa0789610	MtrunA17_Chr6g0451441	28.415	183	115	4	40	207	49	230	3.25e-14	71.6
Msa0789610	MtrunA17_Chr2g0291591	25.100	251	155	7	23	252	19	257	4.62e-13	68.6
Msa0789610	MtrunA17_Chr2g0296781	29.630	135	87	2	122	252	60	190	5.70e-13	68.6
Msa0789610	MtrunA17_Chr5g0441581	25.352	213	126	5	63	252	16	218	5.85e-12	65.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0789610	AT4G15090.1	33.333	249	131	6	39	252	1	249	1.94e-33	128
Msa0789610	AT4G15090.5	33.333	249	131	6	39	252	1	249	1.94e-33	128
Msa0789610	AT4G15090.3	33.333	249	131	6	39	252	1	249	1.94e-33	128
Msa0789610	AT4G15090.2	33.333	249	131	6	39	252	1	249	1.94e-33	128
Msa0789610	AT4G15090.4	33.333	249	131	6	39	252	1	249	1.94e-33	128
Msa0789610	AT3G22170.2	28.467	274	123	7	30	252	7	258	4.07e-27	110
Msa0789610	AT3G22170.1	28.467	274	123	7	30	252	7	258	4.07e-27	110
Msa0789610	AT1G76320.2	32.292	192	102	5	83	252	1	186	2.66e-24	102
Msa0789610	AT1G76320.1	32.292	192	102	5	83	252	1	186	2.74e-24	102
Msa0789610	AT1G76320.4	32.292	192	102	5	83	252	1	186	2.74e-24	102

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Find 37 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCAAAATTTATGCCACTC+TGG	0.565072	5_2:-78931806	Msa0789610:CDS
AATGATGACAATGTTATTGA+TGG	0.455364	5_2:-78932294	Msa0789610:CDS
AATTAATGGAAGTTGTTGAT+GGG	0.547188	5_2:-78932317	Msa0789610:CDS
ACTACTGTAACATAAATCTT+CGG	0.535461	5_2:+78932623	None:intergenic
ACTCTGGCCAAACAATATGG+AGG	0.695583	5_2:-78931790	Msa0789610:CDS
ACTGTAACATAAATCTTCGG+TGG	0.635445	5_2:+78932626	None:intergenic
ACTTATGCTTGCACAAGATA+AGG	0.452056	5_2:-78932060	Msa0789610:CDS
AGAAAATAATGTGCATATGC+TGG	0.444091	5_2:+78931898	None:intergenic
AGAAATCATTTAGATATAGT+CGG	0.576258	5_2:-78931733	Msa0789610:CDS
AGAATGATTCATGTACGGTT+GGG	0.505397	5_2:-78932239	Msa0789610:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCTATCATTCTTAAAGGTA+TGG	-	chr5_2:78931714-78931733	Msa0789610:CDS	30.0%
GAATTAATGGAAGTTGTTGA+TGG	-	chr5_2:78931990-78932009	Msa0789610:CDS	30.0%
TGTTGAGAATGATTCATGTA+CGG	-	chr5_2:78932064-78932083	Msa0789610:CDS	30.0%
TCAAATTCCATTCCAATGTA+TGG	+	chr5_2:78932110-78932129	None:intergenic	30.0%
TAAAATTAGTCGTCGTTCAA+AGG	-	chr5_2:78932194-78932213	Msa0789610:CDS	30.0%
AAATTTGCGTCGTTGTTTAA+AGG	-	chr5_2:78932295-78932314	Msa0789610:CDS	30.0%
AGAAAATAATGTGCATATGC+TGG	+	chr5_2:78932413-78932432	None:intergenic	30.0%
ATTGAAACTATGCAAGATGT+TGG	-	chr5_2:78932470-78932489	Msa0789610:intron	30.0%
TTCATATCCTCCATATTGTT+TGG	+	chr5_2:78932528-78932547	None:intergenic	30.0%
TGCTATCATTCTTAAAGGTA+TGG	-	chr5_2:78931714-78931733	Msa0789610:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	78931619	78932711	78931619	ID=Msa0789610;Name=Msa0789610
chr5_2	mRNA	78931619	78932711	78931619	ID=Msa0789610-mRNA-1;Parent=Msa0789610;Name=Msa0789610-mRNA-1;_AED=0.19;_eAED=0.32;_QI=0\|0\|0\|0.8\|0.25\|0.2\|5\|0\|257
chr5_2	exon	78932595	78932711	78932595	ID=Msa0789610-mRNA-1:exon:23644;Parent=Msa0789610-mRNA-1
chr5_2	exon	78932161	78932340	78932161	ID=Msa0789610-mRNA-1:exon:23643;Parent=Msa0789610-mRNA-1
chr5_2	exon	78932022	78932114	78932022	ID=Msa0789610-mRNA-1:exon:23642;Parent=Msa0789610-mRNA-1
chr5_2	exon	78931776	78932013	78931776	ID=Msa0789610-mRNA-1:exon:23641;Parent=Msa0789610-mRNA-1
chr5_2	exon	78931619	78931764	78931619	ID=Msa0789610-mRNA-1:exon:23640;Parent=Msa0789610-mRNA-1
chr5_2	CDS	78932595	78932711	78932595	ID=Msa0789610-mRNA-1:cds;Parent=Msa0789610-mRNA-1
chr5_2	CDS	78932161	78932340	78932161	ID=Msa0789610-mRNA-1:cds;Parent=Msa0789610-mRNA-1
chr5_2	CDS	78932022	78932114	78932022	ID=Msa0789610-mRNA-1:cds;Parent=Msa0789610-mRNA-1
chr5_2	CDS	78931776	78932013	78931776	ID=Msa0789610-mRNA-1:cds;Parent=Msa0789610-mRNA-1
chr5_2	CDS	78931619	78931764	78931619	ID=Msa0789610-mRNA-1:cds;Parent=Msa0789610-mRNA-1

Gene Sequence

>Msa0789610

ATGAAAATTGGTATTGTCAAATTCTGTGACAAACTCCCTTCAACACACGCTCACACACAAAAGCCACCGAAGATTTATGTTACAGTAGTGCACTCTGCTATCATTCTTAAAGGTATGGAATTAATGGAAGTTGTTGATGGGAATGATGACAATGTTATTGATGGGAATGAAGAGCAATCTTTCACTGAGATTGTTGAGAATGATTCATGTACGGTTGGGAGGCTAATGATAGAGCCATACATTGGAATGGAATTTGATTCACAAGAAAATCCTTATTCATTCTACACTCATTACGCTGTTTATAGAGAGTTTATTGATGTAAGTTATGCACTTATGCTTGCACAAGATAAGGGAAGACGCGGGAATCCACTGCACAAAATTTGCGTCGTTGTGGAATGTGAGGCTAGCTTGCGTATTAAAAAAAATTGTGATGGAAAATGGATTGTTCACAACTTCATTAAAGACCATAATCATGAGCTTTTTCCAGCATATGCACATTATTTTCTTTGCCATAGAGGAATCAATAAAACTCAAAAGCATTGTATTGAAACTATGCAAGATGTTGGAGTGAGAACAAGCAAAATTTATGCCACTCTGGCCAAACAATATGGAGGATATGAAAAAATTGAAAGATATCAGAAATCATTTAGATATAGTCGGCGTTTGACATTAGAATTGGGAGATGCAAATGCAATGTTGGACTGTTTTATGCTCATGCAAGAAGAAAATCCAAGATTCTTTTATGCAATTGATTTGGTCAAAACAACGATTTGA

Protein sequence

>Msa0789610

MKIGIVKFCDKLPSTHAHTQKPPKIYVTVVHSAIILKGMELMEVVDGNDDNVIDGNEEQSFTEIVENDSCTVGRLMIEPYIGMEFDSQENPYSFYTHYAVYREFIDVSYALMLAQDKGRRGNPLHKICVVVECEASLRIKKNCDGKWIVHNFIKDHNHELFPAYAHYFLCHRGINKTQKHCIETMQDVGVRTSKIYATLAKQYGGYEKIERYQKSFRYSRRLTLELGDANAMLDCFMLMQEENPRFFYAIDLVKTTI*