Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791560 | XP_013468829.1 | 94.969 | 159 | 8 | 0 | 1 | 159 | 1 | 159 | 9.51e-98 | 288 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791560 | sp|Q9FJK3|AGL80_ARATH | 46.154 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 3.53e-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791560 | A0A072VMQ1 | 94.969 | 159 | 8 | 0 | 1 | 159 | 1 | 159 | 4.54e-98 | 288 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0791560 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791560 | MtrunA17_Chr1g0188661 | 94.969 | 159 | 8 | 0 | 1 | 159 | 1 | 159 | 8.75e-102 | 288 |
| Msa0791560 | MtrunA17_Chr7g0218151 | 89.937 | 159 | 16 | 0 | 1 | 159 | 1 | 159 | 5.09e-96 | 274 |
| Msa0791560 | MtrunA17_Chr4g0012761 | 86.792 | 159 | 21 | 0 | 1 | 159 | 1 | 159 | 1.04e-93 | 268 |
| Msa0791560 | MtrunA17_Chr1g0188681 | 70.701 | 157 | 46 | 0 | 1 | 157 | 1 | 157 | 8.21e-79 | 230 |
| Msa0791560 | MtrunA17_Chr5g0420351 | 70.513 | 156 | 46 | 0 | 1 | 156 | 1 | 156 | 5.57e-77 | 226 |
| Msa0791560 | MtrunA17_Chr1g0193311 | 74.359 | 156 | 40 | 0 | 1 | 156 | 1 | 156 | 1.74e-75 | 222 |
| Msa0791560 | MtrunA17_Chr1g0188651 | 74.843 | 159 | 38 | 1 | 1 | 159 | 1 | 157 | 7.98e-75 | 220 |
| Msa0791560 | MtrunA17_Chr1g0197261 | 56.129 | 155 | 68 | 0 | 1 | 155 | 1 | 155 | 3.21e-59 | 181 |
| Msa0791560 | MtrunA17_Chr1g0197321 | 55.128 | 156 | 69 | 1 | 1 | 155 | 1 | 156 | 1.16e-57 | 177 |
| Msa0791560 | MtrunA17_Chr1g0197461 | 54.902 | 153 | 69 | 0 | 3 | 155 | 2 | 154 | 5.63e-56 | 172 |
| Msa0791560 | MtrunA17_Chr3g0110131 | 51.592 | 157 | 75 | 1 | 1 | 156 | 1 | 157 | 1.72e-41 | 139 |
| Msa0791560 | MtrunA17_Chr3g0107381 | 45.752 | 153 | 82 | 1 | 1 | 152 | 1 | 153 | 1.01e-38 | 131 |
| Msa0791560 | MtrunA17_Chr5g0432691 | 39.103 | 156 | 94 | 1 | 1 | 155 | 1 | 156 | 2.76e-37 | 125 |
| Msa0791560 | MtrunA17_Chr4g0014321 | 38.065 | 155 | 94 | 2 | 1 | 153 | 1 | 155 | 6.42e-37 | 126 |
| Msa0791560 | MtrunA17_Chr3g0107351 | 42.857 | 154 | 87 | 1 | 3 | 155 | 2 | 155 | 8.38e-34 | 116 |
| Msa0791560 | MtrunA17_Chr3g0107331 | 42.038 | 157 | 90 | 1 | 3 | 158 | 2 | 158 | 1.02e-33 | 116 |
| Msa0791560 | MtrunA17_Chr4g0014031 | 44.667 | 150 | 81 | 2 | 1 | 148 | 1 | 150 | 2.82e-33 | 117 |
| Msa0791560 | MtrunA17_Chr8g0351751 | 40.252 | 159 | 91 | 2 | 1 | 155 | 1 | 159 | 6.97e-33 | 117 |
| Msa0791560 | MtrunA17_Chr4g0013041 | 37.419 | 155 | 93 | 3 | 1 | 153 | 1 | 153 | 9.30e-33 | 115 |
| Msa0791560 | MtrunA17_Chr3g0107361 | 41.558 | 154 | 89 | 1 | 3 | 155 | 2 | 155 | 2.24e-32 | 113 |
| Msa0791560 | MtrunA17_Chr3g0107341 | 40.260 | 154 | 91 | 1 | 3 | 155 | 2 | 155 | 7.84e-31 | 109 |
| Msa0791560 | MtrunA17_Chr4g0014121 | 43.750 | 160 | 88 | 2 | 1 | 158 | 29 | 188 | 3.61e-30 | 108 |
| Msa0791560 | MtrunA17_Chr2g0283941 | 42.038 | 157 | 87 | 3 | 1 | 154 | 1 | 156 | 1.97e-29 | 107 |
| Msa0791560 | MtrunA17_Chr4g0014131 | 46.000 | 150 | 79 | 2 | 1 | 148 | 1 | 150 | 2.96e-29 | 107 |
| Msa0791560 | MtrunA17_Chr3g0107461 | 35.625 | 160 | 99 | 3 | 1 | 157 | 1 | 159 | 2.64e-28 | 102 |
| Msa0791560 | MtrunA17_Chr2g0296831 | 44.068 | 118 | 65 | 1 | 1 | 117 | 1 | 118 | 5.00e-27 | 98.2 |
| Msa0791560 | MtrunA17_Chr3g0105791 | 35.878 | 131 | 82 | 2 | 29 | 157 | 1 | 131 | 3.38e-22 | 86.7 |
| Msa0791560 | MtrunA17_Chr4g0031421 | 35.878 | 131 | 82 | 2 | 29 | 157 | 1 | 131 | 5.03e-22 | 85.9 |
| Msa0791560 | MtrunA17_Chr2g0296861 | 32.258 | 155 | 104 | 1 | 1 | 154 | 1 | 155 | 1.58e-21 | 85.1 |
| Msa0791560 | MtrunA17_Chr4g0008701 | 35.833 | 120 | 73 | 2 | 1 | 116 | 1 | 120 | 1.95e-17 | 78.2 |
| Msa0791560 | MtrunA17_Chr7g0267601 | 36.283 | 113 | 68 | 2 | 1 | 109 | 1 | 113 | 1.23e-16 | 75.9 |
| Msa0791560 | MtrunA17_Chr3g0106501 | 32.061 | 131 | 87 | 2 | 29 | 157 | 1 | 131 | 1.23e-16 | 72.4 |
| Msa0791560 | MtrunA17_Chr3g0106421 | 32.308 | 130 | 84 | 3 | 29 | 155 | 1 | 129 | 2.58e-16 | 72.8 |
| Msa0791560 | MtrunA17_Chr1g0161931 | 41.489 | 94 | 54 | 1 | 64 | 156 | 28 | 121 | 1.34e-13 | 63.5 |
| Msa0791560 | MtrunA17_Chr1g0162261 | 38.776 | 98 | 59 | 1 | 62 | 158 | 20 | 117 | 1.44e-13 | 63.5 |
| Msa0791560 | MtrunA17_Chr1g0162181 | 39.796 | 98 | 58 | 1 | 62 | 158 | 20 | 117 | 4.99e-13 | 62.0 |
| Msa0791560 | MtrunA17_Chr6g0449861 | 31.250 | 112 | 71 | 3 | 1 | 106 | 9 | 120 | 1.43e-12 | 63.9 |
| Msa0791560 | MtrunA17_Chr3g0128721 | 26.974 | 152 | 106 | 2 | 3 | 151 | 6 | 155 | 9.43e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791560 | AT5G48670.1 | 46.154 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 3.59e-35 | 125 |
| Msa0791560 | AT3G05860.2 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 2.25e-32 | 114 |
| Msa0791560 | AT3G05860.3 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 1.09e-31 | 114 |
| Msa0791560 | AT3G05860.1 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 1.88e-31 | 114 |
| Msa0791560 | AT5G26630.1 | 40.000 | 160 | 95 | 1 | 1 | 159 | 1 | 160 | 4.14e-31 | 112 |
| Msa0791560 | AT1G65300.1 | 35.374 | 147 | 94 | 1 | 3 | 148 | 2 | 148 | 5.81e-27 | 102 |
| Msa0791560 | AT1G65330.1 | 36.054 | 147 | 93 | 1 | 3 | 148 | 2 | 148 | 2.54e-26 | 100 |
| Msa0791560 | AT5G26650.1 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 2.53e-23 | 94.4 |
| Msa0791560 | AT5G27960.1 | 41.026 | 117 | 66 | 2 | 4 | 119 | 2 | 116 | 1.76e-22 | 91.7 |
| Msa0791560 | AT5G27810.1 | 43.750 | 96 | 53 | 1 | 29 | 123 | 1 | 96 | 4.21e-21 | 83.2 |
| Msa0791560 | AT2G28700.1 | 34.868 | 152 | 92 | 2 | 1 | 148 | 1 | 149 | 1.63e-20 | 86.3 |
| Msa0791560 | AT1G31630.1 | 31.429 | 140 | 94 | 2 | 3 | 141 | 2 | 140 | 8.16e-20 | 84.3 |
| Msa0791560 | AT1G22590.2 | 32.174 | 115 | 77 | 1 | 1 | 114 | 1 | 115 | 4.23e-19 | 79.3 |
| Msa0791560 | AT1G31640.1 | 35.294 | 136 | 86 | 2 | 3 | 137 | 2 | 136 | 1.94e-18 | 81.6 |
| Msa0791560 | AT5G26580.1 | 36.975 | 119 | 60 | 2 | 1 | 119 | 1 | 104 | 3.29e-17 | 77.4 |
| Msa0791560 | AT2G40210.1 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 1.57e-13 | 67.4 |
| Msa0791560 | AT5G58890.1 | 33.333 | 111 | 66 | 3 | 1 | 104 | 1 | 110 | 2.66e-12 | 63.5 |
| Msa0791560 | AT5G06500.1 | 32.877 | 146 | 85 | 5 | 1 | 141 | 1 | 138 | 7.26e-12 | 61.6 |
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTGAATCATTAGTGATAA+AGG | 0.307446 | 5_3:-2565677 | None:intergenic |
| AGCTGAACATTCGTATGATT+TGG | 0.323437 | 5_3:+2565983 | Msa0791560:CDS |
| GTTGCAAAAGGAAACTTTCC+TGG | 0.331570 | 5_3:-2565903 | None:intergenic |
| CACTAATGATTCAGCAAGAA+AGG | 0.401894 | 5_3:+2565684 | Msa0791560:CDS |
| AAATCTGAAGGAAGCAAAAC+AGG | 0.418438 | 5_3:+2565828 | Msa0791560:CDS |
| TCTCAGTTGATCCCTAGCTT+TGG | 0.420289 | 5_3:-2565932 | None:intergenic |
| CTTTGGTAATCTGTTGCAAA+AGG | 0.444012 | 5_3:-2565915 | None:intergenic |
| TGTTTCTGATTTGAAAGACT+TGG | 0.473410 | 5_3:+2566041 | Msa0791560:CDS |
| TTTCACCTTCTTCCTCGTCA+TGG | 0.482331 | 5_3:-2565650 | None:intergenic |
| TCAGAAACAGTCATATCATC+TGG | 0.489973 | 5_3:-2566027 | None:intergenic |
| AACAGGTGATTGAGAGGTAT+CGG | 0.521473 | 5_3:+2565845 | Msa0791560:CDS |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 5_3:+2565748 | Msa0791560:CDS |
| CGAAGCCATGACGAGGAAGA+AGG | 0.526668 | 5_3:+2565645 | None:intergenic |
| GCAACAGATTACCAAAGCTA+GGG | 0.531580 | 5_3:+2565921 | Msa0791560:CDS |
| TGCAACAGATTACCAAAGCT+AGG | 0.533275 | 5_3:+2565920 | Msa0791560:CDS |
| GAAGAAAAGTATCATCAAGA+AGG | 0.556586 | 5_3:+2565723 | Msa0791560:CDS |
| TCGGAAATTATAGCACATGC+TGG | 0.559487 | 5_3:-2565775 | None:intergenic |
| AGAGGTGTGGCCAAATCTGA+AGG | 0.570010 | 5_3:+2565816 | Msa0791560:CDS |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.572219 | 5_3:+2565701 | Msa0791560:CDS |
| GAAGGACATTGATGACAAGA+TGG | 0.586320 | 5_3:+2566086 | Msa0791560:CDS |
| AAGCAAAACAGGTGATTGAG+AGG | 0.611042 | 5_3:+2565839 | Msa0791560:CDS |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 5_3:+2565803 | Msa0791560:CDS |
| TACAGTGCGAAGCCATGACG+AGG | 0.644898 | 5_3:+2565638 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAATTGCAAAAAATATGA+AGG | + | chr5_3:2566068-2566087 | Msa0791560:CDS | 20.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+CGG | - | chr5_3:2565797-2565816 | None:intergenic | 25.0% |
| TGCTGAATCATTAGTGATAA+AGG | - | chr5_3:2565680-2565699 | None:intergenic | 30.0% | |
| !! | GAAGAAAAGTATCATCAAGA+AGG | + | chr5_3:2565723-2565742 | Msa0791560:CDS | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | + | chr5_3:2565798-2565817 | Msa0791560:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | - | chr5_3:2565802-2565821 | None:intergenic | 30.0% |
| ! | TGTTTCTGATTTGAAAGACT+TGG | + | chr5_3:2566041-2566060 | Msa0791560:CDS | 30.0% |
| CACTAATGATTCAGCAAGAA+AGG | + | chr5_3:2565684-2565703 | Msa0791560:CDS | 35.0% | |
| ! | ACTTTTCTTCCTCTTCTTGT+AGG | - | chr5_3:2565713-2565732 | None:intergenic | 35.0% |
| AGAGAACTCACTATTCTTTG+TGG | + | chr5_3:2565748-2565767 | Msa0791560:CDS | 35.0% | |
| !! | TTGATTCTAAAACAGAGGTG+TGG | + | chr5_3:2565803-2565822 | Msa0791560:CDS | 35.0% |
| ! | TGTTTTGCTTCCTTCAGATT+TGG | - | chr5_3:2565829-2565848 | None:intergenic | 35.0% |
| AAATCTGAAGGAAGCAAAAC+AGG | + | chr5_3:2565828-2565847 | Msa0791560:CDS | 35.0% | |
| TGAGAGAAAAAATGTGAACC+AGG | + | chr5_3:2565885-2565904 | Msa0791560:CDS | 35.0% | |
| ! | CTTTGGTAATCTGTTGCAAA+AGG | - | chr5_3:2565918-2565937 | None:intergenic | 35.0% |
| AGCTGAACATTCGTATGATT+TGG | + | chr5_3:2565983-2566002 | Msa0791560:CDS | 35.0% | |
| TCAGAAACAGTCATATCATC+TGG | - | chr5_3:2566030-2566049 | None:intergenic | 35.0% | |
| TCGGAAATTATAGCACATGC+TGG | - | chr5_3:2565778-2565797 | None:intergenic | 40.0% | |
| AAGCAAAACAGGTGATTGAG+AGG | + | chr5_3:2565839-2565858 | Msa0791560:CDS | 40.0% | |
| ! | AACAGGTGATTGAGAGGTAT+CGG | + | chr5_3:2565845-2565864 | Msa0791560:CDS | 40.0% |
| GTTGCAAAAGGAAACTTTCC+TGG | - | chr5_3:2565906-2565925 | None:intergenic | 40.0% | |
| TGCAACAGATTACCAAAGCT+AGG | + | chr5_3:2565920-2565939 | Msa0791560:CDS | 40.0% | |
| GCAACAGATTACCAAAGCTA+GGG | + | chr5_3:2565921-2565940 | Msa0791560:CDS | 40.0% | |
| ! | GAAGGACATTGATGACAAGA+TGG | + | chr5_3:2566086-2566105 | Msa0791560:CDS | 40.0% |
| GAAAGGCTTCCTACAAGAAG+AGG | + | chr5_3:2565701-2565720 | Msa0791560:CDS | 45.0% | |
| TCTCAGTTGATCCCTAGCTT+TGG | - | chr5_3:2565935-2565954 | None:intergenic | 45.0% | |
| ! | AGAGGTGTGGCCAAATCTGA+AGG | + | chr5_3:2565816-2565835 | Msa0791560:CDS | 50.0% |
| !!! | CTGGCACAGTGTTGTTTTGC+AGG | - | chr5_3:2566011-2566030 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 2565652 | 2566131 | 2565652 | ID=Msa0791560;Name=Msa0791560 |
| chr5_3 | mRNA | 2565652 | 2566131 | 2565652 | ID=Msa0791560-mRNA-1;Parent=Msa0791560;Name=Msa0791560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|159 |
| chr5_3 | exon | 2565652 | 2566131 | 2565652 | ID=Msa0791560-mRNA-1:exon:1102;Parent=Msa0791560-mRNA-1 |
| chr5_3 | CDS | 2565652 | 2566131 | 2565652 | ID=Msa0791560-mRNA-1:cds;Parent=Msa0791560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
