AlfalfaGEDB | Msa0796730

M. sativa cultivar zhongmu-4 / Msa0796730

KEGG: K07466
GO: -
PFAM: PF02721.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0796730	sp\|O04086\|Y1105_ARATH	59.770	87	28	3	421	500	514	600	4.02e-23	106
Msa0796730	sp\|O04086\|Y1105_ARATH	48.750	80	41	0	345	424	282	361	1.15e-14	80.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0796730	G7K1P7	50.470	319	144	5	5	322	2	307	3.06e-95	308

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0796730	MtrunA17_Chr6g0469931	54.231	260	97	6	5	259	2	244	6.86e-80	262
Msa0796730	MtrunA17_Chr1g0159611	56.497	177	66	4	337	506	367	539	6.79e-46	169
Msa0796730	MtrunA17_Chr1g0159611	76.543	81	17	1	338	418	221	299	4.28e-29	120
Msa0796730	MtrunA17_Chr6g0469811	50.704	142	69	1	5	146	11	151	3.51e-39	139
Msa0796730	MtrunA17_Chr2g0277731	56.667	90	32	1	418	500	527	616	2.32e-26	113
Msa0796730	MtrunA17_Chr2g0277731	38.168	131	67	2	345	474	291	408	4.41e-19	90.5
Msa0796730	MtrunA17_Chr2g0278111	26.481	287	197	4	15	298	11	286	1.13e-24	104
Msa0796730	MtrunA17_Chr5g0411141	26.481	287	197	4	15	298	12	287	1.22e-24	104
Msa0796730	MtrunA17_Chr7g0259081	35.429	175	105	3	9	183	6	172	1.46e-24	100
Msa0796730	MtrunA17_Chr7g0234721	36.000	175	104	3	9	183	6	172	1.39e-23	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0796730	AT1G11050.1	59.770	87	28	3	421	500	514	600	4.09e-24	106
Msa0796730	AT1G11050.1	48.750	80	41	0	345	424	282	361	1.16e-15	80.5
Msa0796730	AT1G52950.1	28.571	308	191	12	21	320	19	305	4.85e-20	94.0
Msa0796730	AT2G48010.1	51.515	66	32	0	353	418	278	343	7.76e-14	74.7
Msa0796730	AT5G18500.2	30.412	194	119	5	227	417	45	225	4.81e-13	71.6
Msa0796730	AT5G18500.5	30.412	194	119	5	227	417	45	225	4.81e-13	71.6
Msa0796730	AT5G18500.4	30.412	194	119	5	227	417	45	225	4.81e-13	71.6
Msa0796730	AT5G18500.1	30.412	194	119	5	227	417	45	225	4.81e-13	71.6
Msa0796730	AT5G18500.3	30.412	194	119	5	227	417	45	225	4.81e-13	71.6
Msa0796730	AT5G18500.6	30.412	194	119	5	227	417	45	225	4.81e-13	71.6

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Find 105 sgRNAs with CRISPR-Local

Find 353 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGTGAGACTGCTCTGTT+TGG	0.410986	5_3:+8709386	Msa0796730:CDS
AACAAGGATGGACTATTATG+AGG	0.522416	5_3:+8710851	Msa0796730:CDS
AACACTTTCAGATGGAACTT+TGG	0.455446	5_3:+8713246	Msa0796730:CDS
AACTTTGACTAATATAGTTG+AGG	0.545314	5_3:+8708981	Msa0796730:CDS
AAGAAATCTTCATCTAATGA+TGG	0.532109	5_3:-8709859	None:intergenic
AAGAATATGATAGGTCAAGG+TGG	0.696121	5_3:+8713202	Msa0796730:CDS
AATCCAAACATGGGAAGCAA+GGG	0.605138	5_3:-8708958	None:intergenic
AATTCTTTGTGCTCATGCAA+TGG	0.562123	5_3:+8713945	Msa0796730:CDS
AATTGTAGCACAAACAATAA+AGG	0.429279	5_3:-8709938	None:intergenic
AATTGTTGTCCACAGTTTGA+TGG	0.338565	5_3:-8709008	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTCAAAGTTAATCCAAACAT+GGG	-	chr5_3:8708970-8708989	None:intergenic	30.0%
AGTCAAAGTTAATCCAAACA+TGG	-	chr5_3:8708971-8708990	None:intergenic	30.0%
GTGGACAACAATTATTTGAT+TGG	+	chr5_3:8709018-8709037	Msa0796730:CDS	30.0%
CTTAATGACTGTAATATCAG+AGG	+	chr5_3:8709179-8709198	Msa0796730:CDS	30.0%
CCAAACAAAAGTTTAAACGA+TGG	-	chr5_3:8709329-8709348	None:intergenic	30.0%
TATCCGGATTGATCATAATT+CGG	-	chr5_3:8709645-8709664	None:intergenic	30.0%
TAACATCCAAACTGATATTG+GGG	-	chr5_3:8709789-8709808	None:intergenic	30.0%
ATTGTTTGTGCTACAATTGA+TGG	+	chr5_3:8709943-8709962	Msa0796730:CDS	30.0%
AGAGCAACACTTGATTATTA+TGG	+	chr5_3:8710787-8710806	Msa0796730:CDS	30.0%
CTGCTATATCCAATGAAATA+TGG	+	chr5_3:8710896-8710915	Msa0796730:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	8708538	8714104	8708538	ID=Msa0796730;Name=Msa0796730
chr5_3	mRNA	8708538	8714104	8708538	ID=Msa0796730-mRNA-1;Parent=Msa0796730;Name=Msa0796730-mRNA-1;_AED=0.40;_eAED=0.41;_QI=0\|0\|0.11\|1\|0.75\|0.55\|9\|0\|510
chr5_3	exon	8708538	8708699	8708538	ID=Msa0796730-mRNA-1:exon:3688;Parent=Msa0796730-mRNA-1
chr5_3	exon	8708772	8709043	8708772	ID=Msa0796730-mRNA-1:exon:3689;Parent=Msa0796730-mRNA-1
chr5_3	exon	8709155	8709265	8709155	ID=Msa0796730-mRNA-1:exon:3690;Parent=Msa0796730-mRNA-1
chr5_3	exon	8709382	8709518	8709382	ID=Msa0796730-mRNA-1:exon:3691;Parent=Msa0796730-mRNA-1
chr5_3	exon	8709608	8709692	8709608	ID=Msa0796730-mRNA-1:exon:3692;Parent=Msa0796730-mRNA-1
chr5_3	exon	8709798	8709964	8709798	ID=Msa0796730-mRNA-1:exon:3693;Parent=Msa0796730-mRNA-1
chr5_3	exon	8710761	8710872	8710761	ID=Msa0796730-mRNA-1:exon:3694;Parent=Msa0796730-mRNA-1
chr5_3	exon	8713168	8713376	8713168	ID=Msa0796730-mRNA-1:exon:3695;Parent=Msa0796730-mRNA-1
chr5_3	exon	8713827	8714104	8713827	ID=Msa0796730-mRNA-1:exon:3696;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8708538	8708699	8708538	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8708772	8709043	8708772	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8709155	8709265	8709155	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8709382	8709518	8709382	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8709608	8709692	8709608	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8709798	8709964	8709798	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8710761	8710872	8710761	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8713168	8713376	8713168	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1
chr5_3	CDS	8713827	8714104	8713827	ID=Msa0796730-mRNA-1:cds;Parent=Msa0796730-mRNA-1

Gene Sequence

>Msa0796730

CTGTGTGAGTCTGATCGCAACTTTAATTTGCTGTCTGATGTTGTTCCTGGATGGGATCGGTCTTGGAGATTCATGCTTCGTGTTGTTCGTTTGTGGGAGGTGCCAAATTTTCTTCATCCAGGGCAGATCAATTCGATTGAGATGGTGTTTGTTGATGAGAAGGGTGTTAAGATTCACGCGACTGTCAGAAAACAATTGTTGTACCTTTTCCATAAGAAGTTGGTTGAAGGTCAGGTGTATAAGATGTCATATCTTTCAGTTGCTCTTAGCAGTTGTTCTTACAGGACAACTACCCATACTTATAAGTTGTTTTTTCAGATGAGGACCAAAGTGAATGTTGTTGAGGGTCCCTTGCTTCCCATGTTTGGATTAACTTTGACTAATATAGTTGAGGTTAACCATCAAACTGTGGACAACAATTATTTGATTGGTTTTTTTTGCTGTTTAGATGACATTGGCTTAATGACTGTAATATCAGAGGTAAGGGAGTATATTAAGGATGGGAAGATCACCAAGATGATTTTGCTTCAGCTAACTGATCACAGTGGGAAATGTGAGACTGCTCTGTTTGGAGATTATGTGGATCACTTGAAACAACTCATGGCCAAAGTTGATGAAGGTGTGCCTGTGCTGGTTATGCATGTGCTGGTTATGCAATTCGCAAAAGTGAAGATTTTCAAAGAGAATGCATCCCTGCAAAATGTAATGAATACTACCCGAATTATGATCAATCCGGATATATCAGAGGCTGCTGAGTTTAAGAAAGGTTTGGATGTTAATTCAATTGGAAGCAGCTCCATTGTGCCGCTTATTGGTCTGAGGGGTAGACCATCATTAGATGAAGATTTCTTGCATCTCCATCCAAAGAAGTCTATTGCAGAATCGAAGGAAGTTGGGGAGAGTGATACCTTTATTGTTTGTGCTACAATTGATGCTCTTGATCCTAAGTTGTATTATTATAGAGCAACACTTGATTATTATGGAGTTTTGACGCAATATTCTCATCCAAGGGACACTAAAGCAAAACAAGGATGGACTATTATGAGAGCTAGTGATAAGGCTACAAACAAGTTTTCACAGAAGAATATGATAGGTCAAGGTGGTGATGGTGTTGTGTACAAAGGAACACTTTCAGATGGAACTTTGGTTGCAGTGAAAATCATTTTTTATTTGGATACTAGAGGGGATGAAGAGTTTTGCTATGAAATTGAGATTACTAGCAAAATAAAGCATATAAATATTCTTGCTTTAAGAGTTGTTTTGATCACTGATCGGGCTTGGACACTTGCAAAATCAGGGAAAGTTGAAATATTTGATCAAGCAGTTAAAGATGAAGGGCCGGAGAAAATCATGGAAAGGTTTGTACCTGTTGGAATTCTTTGTGCTCATGCAATGGTTGCATTAAGGCCTACTATTGCTGAGGCAATTAAGATGTTAGAAGGTGATATTGATATTCCTAATTTACCAGATAGGCCTGTTCCACTTGGTCATGAATCTTTTCTATCATATTTGATCCACTTTCCCTTCTGCTAA

Protein sequence

>Msa0796730

LCESDRNFNLLSDVVPGWDRSWRFMLRVVRLWEVPNFLHPGQINSIEMVFVDEKGVKIHATVRKQLLYLFHKKLVEGQVYKMSYLSVALSSCSYRTTTHTYKLFFQMRTKVNVVEGPLLPMFGLTLTNIVEVNHQTVDNNYLIGFFCCLDDIGLMTVISEVREYIKDGKITKMILLQLTDHSGKCETALFGDYVDHLKQLMAKVDEGVPVLVMHVLVMQFAKVKIFKENASLQNVMNTTRIMINPDISEAAEFKKGLDVNSIGSSSIVPLIGLRGRPSLDEDFLHLHPKKSIAESKEVGESDTFIVCATIDALDPKLYYYRATLDYYGVLTQYSHPRDTKAKQGWTIMRASDKATNKFSQKNMIGQGGDGVVYKGTLSDGTLVAVKIIFYLDTRGDEEFCYEIEITSKIKHINILALRVVLITDRAWTLAKSGKVEIFDQAVKDEGPEKIMERFVPVGILCAHAMVALRPTIAEAIKMLEGDIDIPNLPDRPVPLGHESFLSYLIHFPFC*