AlfalfaGEDB | Msa0799520

M. sativa cultivar zhongmu-4 / Msa0799520

KEGG: K09264
GO: GO:0000003 GO:0003002 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009791 GO:0009908 GO:0010093 GO:0010097 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:1905392 GO:1905393
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0799520	sp\|Q003J2\|TM6_VITVI	65.948	232	72	4	1	232	1	225	3.80e-100	293

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0799520	A0A072UCQ0	99.138	232	2	0	1	232	1	232	2.17e-170	477

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TFs/TRs:

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Gene ID	Type	Classification
Msa0799520	TF	MADS-MIKC

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0799520	MtrunA17_Chr5g0404951	98.707	232	2	1	1	232	1	231	2.10e-171	471
Msa0799520	MtrunA17_Chr3g0142191	53.846	221	95	5	1	220	1	215	2.59e-74	224
Msa0799520	MtrunA17_Chr3g0125241	47.260	146	73	2	1	146	26	167	2.02e-40	137
Msa0799520	MtrunA17_Chr1g0159661	43.919	148	79	2	1	148	1	144	2.74e-35	123
Msa0799520	MtrunA17_Chr7g0220121	34.234	222	111	8	1	208	1	201	3.05e-31	115
Msa0799520	MtrunA17_Chr8g0351051	38.333	180	96	4	1	173	1	172	3.09e-31	115
Msa0799520	MtrunA17_Chr3g0077501	40.556	180	90	5	1	173	1	170	7.81e-30	110
Msa0799520	MtrunA17_Chr1g0164791	36.000	175	102	2	1	168	1	172	2.70e-29	109
Msa0799520	MtrunA17_Chr8g0380021	40.000	180	91	5	1	173	17	186	2.88e-29	110
Msa0799520	MtrunA17_Chr3g0097101	38.122	181	97	4	1	174	16	188	4.49e-29	109

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0799520	AT3G54340.1	48.182	220	110	2	1	220	1	216	1.46e-64	200
Msa0799520	AT5G20240.2	43.972	141	75	2	1	141	1	137	1.12e-33	119
Msa0799520	AT5G20240.1	41.333	150	84	2	1	150	1	146	1.63e-33	120
Msa0799520	AT2G42830.2	36.453	203	104	5	1	186	16	210	7.36e-32	117
Msa0799520	AT5G60910.1	41.341	179	97	4	1	175	1	175	9.78e-32	116
Msa0799520	AT4G11880.2	41.818	165	81	5	1	158	1	157	2.53e-31	114
Msa0799520	AT4G11880.3	41.818	165	81	5	1	158	1	157	2.53e-31	114
Msa0799520	AT3G58780.5	37.433	187	102	4	1	180	22	200	2.41e-30	113
Msa0799520	AT3G58780.1	37.433	187	102	4	1	180	16	194	3.02e-30	112
Msa0799520	AT4G11880.4	40.984	183	89	6	1	176	1	171	8.66e-30	110

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Find 51 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCGGACTGATACCTGT+AGG	0.623665	5_3:+12757048	Msa0799520:CDS
AAGCAAATTTAACATGACAT+TGG	0.535516	5_3:-12757711	None:intergenic
ACATACTCAAAGAGAAGAAA+TGG	0.436448	5_3:+12755748	Msa0799520:CDS
AGAAGCTTGGAGCAGATGAA+TGG	0.546698	5_3:+12757183	Msa0799520:CDS
AGATCATTGAAAATGGGTCG+TGG	0.568091	5_3:+12755679	Msa0799520:exon
AGGCATAGAATAGGTGAGGG+TGG	0.608397	5_3:+12756265	Msa0799520:intron
AGGTGAGGGTGGTATGGAAT+TGG	0.393769	5_3:+12756276	Msa0799520:CDS
AGTTTCATGTGATCAAAACT+CGG	0.647118	5_3:+12757033	Msa0799520:intron
ATAAGCTTAGAAAACAGATC+AGG	0.539761	5_3:+12756166	Msa0799520:CDS
ATACAGAGTGGAAGCACCAT+TGG	0.546264	5_3:-12757583	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTAGTGAAGATCATTGAAAA+TGG	+	chr5_3:12755672-12755691	Msa0799520:exon	30.0%
ACATACTCAAAGAGAAGAAA+TGG	+	chr5_3:12755748-12755767	Msa0799520:CDS	30.0%
TGATCAATATCAGAAGACTT+TGG	+	chr5_3:12756003-12756022	Msa0799520:CDS	30.0%
GATCAATATCAGAAGACTTT+GGG	+	chr5_3:12756004-12756023	Msa0799520:CDS	30.0%
ATCAATATCAGAAGACTTTG+GGG	+	chr5_3:12756005-12756024	Msa0799520:CDS	30.0%
ATAAGCTTAGAAAACAGATC+AGG	+	chr5_3:12756166-12756185	Msa0799520:CDS	30.0%
GTAGAAAGGGTGAAAATAAA+GGG	-	chr5_3:12756444-12756463	None:intergenic	30.0%
TGTAGAAAGGGTGAAAATAA+AGG	-	chr5_3:12756445-12756464	None:intergenic	30.0%
TTTCTATCTATCAAGCTCTA+AGG	+	chr5_3:12756864-12756883	Msa0799520:intron	30.0%
ATATCAGCTTAAAGACTTCA+AGG	-	chr5_3:12756977-12756996	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	12755608	12757902	12755608	ID=Msa0799520;Name=Msa0799520
chr5_3	mRNA	12755608	12757902	12755608	ID=Msa0799520-mRNA-1;Parent=Msa0799520;Name=Msa0799520-mRNA-1;_AED=0.02;_eAED=0.02;_QI=83\|1\|1\|1\|1\|1\|7\|210\|232
chr5_3	exon	12755608	12755878	12755608	ID=Msa0799520-mRNA-1:exon:5233;Parent=Msa0799520-mRNA-1
chr5_3	exon	12755988	12756057	12755988	ID=Msa0799520-mRNA-1:exon:5234;Parent=Msa0799520-mRNA-1
chr5_3	exon	12756126	12756187	12756126	ID=Msa0799520-mRNA-1:exon:5235;Parent=Msa0799520-mRNA-1
chr5_3	exon	12756267	12756375	12756267	ID=Msa0799520-mRNA-1:exon:5236;Parent=Msa0799520-mRNA-1
chr5_3	exon	12757035	12757076	12757035	ID=Msa0799520-mRNA-1:exon:5237;Parent=Msa0799520-mRNA-1
chr5_3	exon	12757180	12757224	12757180	ID=Msa0799520-mRNA-1:exon:5238;Parent=Msa0799520-mRNA-1
chr5_3	exon	12757510	12757902	12757510	ID=Msa0799520-mRNA-1:exon:5239;Parent=Msa0799520-mRNA-1
chr5_3	five_prime_UTR	12755608	12755690	12755608	ID=Msa0799520-mRNA-1:five_prime_utr;Parent=Msa0799520-mRNA-1
chr5_3	CDS	12755691	12755878	12755691	ID=Msa0799520-mRNA-1:cds;Parent=Msa0799520-mRNA-1
chr5_3	CDS	12755988	12756057	12755988	ID=Msa0799520-mRNA-1:cds;Parent=Msa0799520-mRNA-1
chr5_3	CDS	12756126	12756187	12756126	ID=Msa0799520-mRNA-1:cds;Parent=Msa0799520-mRNA-1
chr5_3	CDS	12756267	12756375	12756267	ID=Msa0799520-mRNA-1:cds;Parent=Msa0799520-mRNA-1
chr5_3	CDS	12757035	12757076	12757035	ID=Msa0799520-mRNA-1:cds;Parent=Msa0799520-mRNA-1
chr5_3	CDS	12757180	12757224	12757180	ID=Msa0799520-mRNA-1:cds;Parent=Msa0799520-mRNA-1
chr5_3	CDS	12757510	12757692	12757510	ID=Msa0799520-mRNA-1:cds;Parent=Msa0799520-mRNA-1
chr5_3	three_prime_UTR	12757693	12757902	12757693	ID=Msa0799520-mRNA-1:three_prime_utr;Parent=Msa0799520-mRNA-1

Gene Sequence

>Msa0799520

ATGGGTCGTGGGAAGATTGAGATAAAGTTGATTGAGAATCCTACTAACAGGCAAGTGACATACTCAAAGAGAAGAAATGGTATATTCAAGAAAGCACATGAACTTAGTGTTCTTTGTGATGCTAAGGTTTCACTCATCATGTTCTCCAAAAATAACAAGATGCATGAGTACATCACCCCTGGACTAACTACAAAGAAGATTATTGATCAATATCAGAAGACTTTGGGGGATATAGATCTGTGGCGTTCTCACTATGAGAAAATGCTTGAAAACTTGAAGAAACTGAAAGATATTAACCATAAGCTTAGAAAACAGATCAGGCATAGAATAGGTGAGGGTGGTATGGAATTGGATGACCTTAGCTTCCAGCAACTGCGTAGTCTTGAAGAGGATATGAATTCTTCCATTGCAAAAATACGTGAAAGGAAGTTTCATGTGATCAAAACTCGGACTGATACCTGTAGGAAAAAGGTTAGAAGCTTGGAGCAGATGAATGGAAATCTCTTGCTTGAACTTAAGGAAAAGTGTGTGATCCATCCACAATTTCTTTTCCACGACGAAGGAGACGAGGAATCAGCAGTTGCATTGGCCAATGGTGCTTCCACTCTGTATGCATTCTGTCAGCACCATTCTCATCTGAATCTTCCTCATCACCATGGAGAAGAAGGTTACAAAAACGACGATTTGCGCCTTGGTTGA

Protein sequence

>Msa0799520

MGRGKIEIKLIENPTNRQVTYSKRRNGIFKKAHELSVLCDAKVSLIMFSKNNKMHEYITPGLTTKKIIDQYQKTLGDIDLWRSHYEKMLENLKKLKDINHKLRKQIRHRIGEGGMELDDLSFQQLRSLEEDMNSSIAKIRERKFHVIKTRTDTCRKKVRSLEQMNGNLLLELKEKCVIHPQFLFHDEGDEESAVALANGASTLYAFCQHHSHLNLPHHHGEEGYKNDDLRLG*