AlfalfaGEDB | Msa0801430

M. sativa cultivar zhongmu-4 / Msa0801430

KEGG: K04730
GO: GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0010065 GO:0010067 GO:0010087 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048731 GO:0048856 GO:0061458
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0801430	PNY02510.1	71.186	118	27	1	70	180	197	314	2.42e-48	172
Msa0801430	PNY02510.1	71.429	91	16	2	4	84	248	338	2.51e-31	127

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0801430	sp\|C0LGP4\|Y3475_ARATH	48.780	123	52	5	69	180	770	892	5.31e-28	113
Msa0801430	sp\|C0LGP4\|Y3475_ARATH	55.224	67	28	1	6	70	826	892	1.09e-16	80.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0801430	A0A067LJH4	39.610	154	51	4	77	188	2	155	4.93e-22	102
Msa0801430	A0A067LJH4	43.578	218	80	5	6	180	52	269	1.15e-48	172

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0801430	MtrunA17_Chr5g0408401	69.748	119	29	1	69	180	547	665	4.06e-51	175
Msa0801430	MtrunA17_Chr5g0408401	72.727	88	18	1	4	91	599	680	4.11e-37	135
Msa0801430	MtrunA17_Chr3g0113221	63.025	119	37	1	69	180	773	891	4.35e-43	153
Msa0801430	MtrunA17_Chr3g0113221	76.119	67	16	0	4	70	825	891	3.79e-31	119
Msa0801430	MtrunA17_Chr5g0408611	60.976	123	40	2	66	180	767	889	3.83e-42	150
Msa0801430	MtrunA17_Chr5g0408611	69.697	66	19	1	6	70	824	889	6.81e-25	100
Msa0801430	MtrunA17_Chr5g0408421	61.667	120	38	2	69	180	764	883	1.81e-41	148
Msa0801430	MtrunA17_Chr5g0408421	66.667	66	21	1	6	70	818	883	5.93e-23	95.5
Msa0801430	MtrunA17_Chr5g0408821	57.724	123	44	2	66	180	173	295	6.72e-41	142
Msa0801430	MtrunA17_Chr5g0408821	55.172	87	32	2	6	91	230	310	2.36e-24	98.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0801430	AT3G47580.1	46.825	126	56	4	66	180	768	893	8.16e-30	115
Msa0801430	AT3G47580.1	56.716	67	27	1	6	70	827	893	4.96e-18	81.6
Msa0801430	AT3G47570.1	48.780	123	52	5	69	180	770	892	5.40e-29	113
Msa0801430	AT3G47570.1	55.224	67	28	1	6	70	826	892	1.11e-17	80.5
Msa0801430	AT3G47110.1	47.967	123	53	4	69	180	787	909	6.14e-29	112
Msa0801430	AT3G47110.1	50.746	67	31	1	6	70	843	909	9.12e-16	75.1
Msa0801430	AT3G47090.1	46.825	126	56	5	66	180	766	891	9.09e-29	112
Msa0801430	AT3G47090.1	53.731	67	29	1	6	70	825	891	1.31e-17	80.5
Msa0801430	AT5G39390.1	43.902	123	58	4	69	180	277	399	1.11e-24	100
Msa0801430	AT5G39390.1	56.716	67	27	1	6	70	333	399	5.52e-18	81.3

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Find 32 sgRNAs with CRISPR-Local

Find 886 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCAAAGTAGCACAAT+TGG	0.385492	5_3:+15368508	Msa0801430:CDS
AAGTAGCACAATTGGAATAA+AGG	0.333164	5_3:+15368516	Msa0801430:CDS
AATCATTCAATCTTGAGCAA+AGG	0.557380	5_3:+15368314	Msa0801430:CDS
ACAGTTGGTTATGCGCCCCT+AGG	0.594017	5_3:+15368541	Msa0801430:CDS
ACTCAAAGTGAAGATAACAA+AGG	0.660469	5_3:-15368366	None:intergenic
AGGTCAAGAATTTAAAGCTC+TGG	0.405873	5_3:+15375955	Msa0801430:CDS
AGTAGCACAATTGGAATAAA+GGG	0.389024	5_3:+15368517	Msa0801430:CDS
AGTAGCTCAATTGGAATAAA+GGG	0.382396	5_3:+15362954	Msa0801430:CDS
ATCACTAACTTTAGCTACCA+TGG	0.519295	5_3:-15368446	None:intergenic
ATTGGAATAAAGGGGACAGT+TGG	0.536010	5_3:+15368526	Msa0801430:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCATTAAGAAGAACATTTGC+AGG	-	chr5_3:15362861-15362880	None:intergenic	30.0%
TGTTCTTCTTAATGACTTCA+TGG	+	chr5_3:15362866-15362885	Msa0801430:CDS	30.0%
AGTAGCTCAATTGGAATAAA+GGG	+	chr5_3:15362954-15362973	Msa0801430:CDS	30.0%
CTGGTATTAGAAATGTTAAC+TGG	+	chr5_3:15363192-15363211	Msa0801430:intron	30.0%
ACAATATGGAAAAGGTTATC+TGG	-	chr5_3:15363285-15363304	None:intergenic	30.0%
TTGATTATCTGACATCATGT+TGG	+	chr5_3:15363668-15363687	Msa0801430:intron	30.0%
TGATTATCTGACATCATGTT+GGG	+	chr5_3:15363669-15363688	Msa0801430:intron	30.0%
TTAACTTCATGTATCACATG+AGG	+	chr5_3:15363902-15363921	Msa0801430:intron	30.0%
TAACTTCATGTATCACATGA+GGG	+	chr5_3:15363903-15363922	Msa0801430:intron	30.0%
ATAGTAGAAGCAATGTTCTT+AGG	+	chr5_3:15363944-15363963	Msa0801430:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	15362790	15375976	15362790	ID=Msa0801430;Name=Msa0801430
chr5_3	mRNA	15362790	15375976	15362790	ID=Msa0801430-mRNA-1;Parent=Msa0801430;Name=Msa0801430-mRNA-1;_AED=0.42;_eAED=0.43;_QI=0\|0\|0\|1\|0.25\|0\|5\|0\|188
chr5_3	exon	15362790	15362796	15362790	ID=Msa0801430-mRNA-1:exon:6382;Parent=Msa0801430-mRNA-1
chr5_3	exon	15362802	15362999	15362802	ID=Msa0801430-mRNA-1:exon:6383;Parent=Msa0801430-mRNA-1
chr5_3	exon	15368212	15368399	15368212	ID=Msa0801430-mRNA-1:exon:6384;Parent=Msa0801430-mRNA-1
chr5_3	exon	15368421	15368562	15368421	ID=Msa0801430-mRNA-1:exon:6385;Parent=Msa0801430-mRNA-1
chr5_3	exon	15375948	15375976	15375948	ID=Msa0801430-mRNA-1:exon:6386;Parent=Msa0801430-mRNA-1
chr5_3	CDS	15362790	15362796	15362790	ID=Msa0801430-mRNA-1:cds;Parent=Msa0801430-mRNA-1
chr5_3	CDS	15362802	15362999	15362802	ID=Msa0801430-mRNA-1:cds;Parent=Msa0801430-mRNA-1
chr5_3	CDS	15368212	15368399	15368212	ID=Msa0801430-mRNA-1:cds;Parent=Msa0801430-mRNA-1
chr5_3	CDS	15368421	15368562	15368421	ID=Msa0801430-mRNA-1:cds;Parent=Msa0801430-mRNA-1
chr5_3	CDS	15375948	15375976	15375948	ID=Msa0801430-mRNA-1:cds;Parent=Msa0801430-mRNA-1

Gene Sequence

>Msa0801430

ATGTTGCCATTTTGTTATCTTCACTACGAATGCGAGCAGCCTGTAATTCATTGTGATTTAAAGCCTGCAAATGTTCTTCTTAATGACTTCATGGTAGCTCAAGTGAGTGATTTTGGGTTAGCAAAGTTGCTCTCAAGTGTTGGAGTCTCTCTGATGCAGAGTAGCTCAATTGGAATAAAGGGGACAGTTGGCTATGCACCCCCAGATTACAAAGGCAATGAGTTCAAAGCTCTCGTATTTGAGTACATGACAAATGGAAACCTAGAAAGTTGGTTGCATCCAACAATAGAAATAACAGATAAGCCCAAATCATTCAATCTTGAGCAAAGGTTAAATGTTATTGCTGATGTTGCTTCAGCCCTTTGTTATCTTCACTTTGAGTGCGAGCAACCTCCGGAAAATGTTCTACTTGATGAATCCATGGTAGCTAAAGTTAGTGATTTTGGCTTAGCAAGGTTGATCTCAGGTGATGAAGTCTCAAAAATGCAAAGTAGCACAATTGGAATAAAGGGGACAGTTGGTTATGCGCCCCTAGATTACAAAGGTCAAGAATTTAAAGCTCTG

Protein sequence

>Msa0801430

MLPFCYLHYECEQPVIHCDLKPANVLLNDFMVAQVSDFGLAKLLSSVGVSLMQSSSIGIKGTVGYAPPDYKGNEFKALVFEYMTNGNLESWLHPTIEITDKPKSFNLEQRLNVIADVASALCYLHFECEQPPENVLLDESMVAKVSDFGLARLISGDEVSKMQSSTIGIKGTVGYAPLDYKGQEFKAL