AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0804670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0804670 MtrunA17_Chr5g0412371 94.231 208 11 1 1 208 1 207 3.57e-143 397
Msa0804670 MtrunA17_Chr5g0427361 40.571 175 82 3 32 184 23 197 2.33e-26 101
Msa0804670 MtrunA17_Chr7g0255961 33.971 209 116 6 7 200 31 232 6.49e-26 100
Msa0804670 MtrunA17_Chr1g0198801 37.126 167 87 3 43 198 156 315 6.67e-25 99.8
Msa0804670 MtrunA17_Chr3g0113721 35.673 171 86 4 45 198 39 202 1.75e-23 93.6
Msa0804670 MtrunA17_Chr2g0325521 39.583 144 72 3 40 177 54 188 2.65e-20 85.5
Msa0804670 MtrunA17_Chr4g0041051 35.256 156 90 2 34 185 57 205 9.07e-18 79.0
Msa0804670 MtrunA17_Chr4g0045901 36.364 143 78 4 39 175 68 203 1.78e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0804670 AT4G38070.1 48.795 166 72 4 20 184 69 222 1.38e-45 152
Msa0804670 AT3G25710.1 41.611 149 77 3 32 177 119 260 1.08e-25 102
Msa0804670 AT1G68810.1 42.012 169 78 3 43 198 172 333 7.44e-25 100
Msa0804670 AT3G56770.1 33.333 174 93 6 40 200 39 202 2.93e-22 90.9
Msa0804670 AT2G40200.1 39.259 135 66 4 46 172 64 190 4.38e-20 85.5
Msa0804670 AT2G41130.1 35.838 173 93 5 39 200 60 225 1.65e-19 84.0

Find 45 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGGCAGACAAATTGAATT+TGG 0.253589 5_3:+20611384 Msa0804670:CDS
TGTCTGCCCCACCAGGAAAC+TGG 0.346018 5_3:-20611372 None:intergenic
TTGGACCCATAACACACTTC+TGG 0.378699 5_3:-20611551 None:intergenic
ACACTAACTTTGATCAAGTT+GGG 0.389149 5_3:-20610998 None:intergenic
AGGTGTTGAAAATGAAGGTT+TGG 0.393224 5_3:+20611573 Msa0804670:CDS
AATGAAAGCCAAAGTTGTTA+AGG 0.399548 5_3:+20611501 Msa0804670:CDS
GAAGAACCAGAAGTGTGTTA+TGG 0.408049 5_3:+20611545 Msa0804670:CDS
CATCTCAACCTTAACAACTT+TGG 0.408942 5_3:-20611509 None:intergenic
AAGAACCAGAAGTGTGTTAT+GGG 0.427563 5_3:+20611546 Msa0804670:CDS
TCAAAGGAGATCCAGTTTCC+TGG 0.436396 5_3:+20611361 Msa0804670:CDS
GACACTAACTTTGATCAAGT+TGG 0.443559 5_3:-20610999 None:intergenic
GATGAGGCGGTATACTCAAT+AGG 0.446628 5_3:+20611645 Msa0804670:CDS
GGTGTTGAAAATGAAGGTTT+GGG 0.450241 5_3:+20611574 Msa0804670:CDS
TTCAATTTGTCTGCCCCACC+AGG 0.456050 5_3:-20611379 None:intergenic
TACCGCCTCATCTTAAAACT+TGG 0.469951 5_3:-20611634 None:intergenic
GATATAGATGACATCAATCC+TGG 0.505784 5_3:-20611463 None:intergenic
AGACGTAGAATGCGAATCAA+TGG 0.510341 5_3:+20610947 Msa0804670:CDS
AGGAAACTGGATCTCCTTTG+AGG 0.538324 5_3:-20611359 None:intergenic
CAAAGTTGTTAAGGTTGAGA+TGG 0.542500 5_3:+20611510 Msa0804670:CDS
GTCCAAGGTGTTGAAAATGA+AGG 0.548376 5_3:+20611568 Msa0804670:CDS
GGAAAAGTGTAATGATGAGG+AGG 0.576454 5_3:+20611405 Msa0804670:CDS
TTGAGTTGTGAGGATAGACC+AGG 0.582755 5_3:+20611445 Msa0804670:CDS
GGAGATCCAGTTTCCTGGTG+GGG 0.584829 5_3:+20611366 Msa0804670:CDS
GGATCTCCTTTGAGGTAGCA+TGG 0.586127 5_3:-20611351 None:intergenic
AGAAGTGTGTTATGGGTCCA+AGG 0.587934 5_3:+20611553 Msa0804670:CDS
CATGTTCGAAATTACTGTAA+CGG 0.590992 5_3:+20610791 Msa0804670:CDS
CACTAACTTTGATCAAGTTG+GGG 0.594450 5_3:-20610997 None:intergenic
GGAGACTATCAAACAAGTGA+AGG 0.601788 5_3:+20611291 Msa0804670:CDS
AGGAGATCCAGTTTCCTGGT+GGG 0.605957 5_3:+20611365 Msa0804670:CDS
GTTGAGATGGTGACAGTGGG+TGG 0.609342 5_3:+20611523 Msa0804670:CDS
GAAAAGTGTAATGATGAGGA+GGG 0.610839 5_3:+20611406 Msa0804670:CDS
TAAGGTTGAGATGGTGACAG+TGG 0.619246 5_3:+20611519 Msa0804670:CDS
TAACTTTGATCAAGTTGGGG+AGG 0.625634 5_3:-20610994 None:intergenic
GGACAAGGCATCAATACTAG+TGG 0.628919 5_3:+20611270 Msa0804670:CDS
TATCTCGAAGCAAAGATCAA+AGG 0.632574 5_3:+20610877 Msa0804670:CDS
AGATAGCCATGCTACCTCAA+AGG 0.632769 5_3:+20611345 Msa0804670:CDS
TATTGATTGACAGAAGGACA+AGG 0.644249 5_3:+20611255 Msa0804670:intron
AAGGTTGAGATGGTGACAGT+GGG 0.645311 5_3:+20611520 Msa0804670:CDS
AAGGAGATCCAGTTTCCTGG+TGG 0.648546 5_3:+20611364 Msa0804670:CDS
CTCGAAGCAAAGATCAAAGG+CGG 0.651214 5_3:+20610880 Msa0804670:CDS
AGTTGGGGAGGATACTGCGG+AGG 0.655539 5_3:-20610982 None:intergenic
TTTGGAAAAGTGTAATGATG+AGG 0.678742 5_3:+20611402 Msa0804670:CDS
GAAAGCTACATTGAGTTGTG+AGG 0.689461 5_3:+20611435 Msa0804670:CDS
TCAAGTTGGGGAGGATACTG+CGG 0.696028 5_3:-20610985 None:intergenic
GTTGGGGAGGATACTGCGGA+GGG 0.717546 5_3:-20610981 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTGTTTTCATAAAGT+TGG - chr5_3:20611042-20611061 None:intergenic 15.0%
!!! AAATTTTGTTTTCATAAAGT+TGG - chr5_3:20611042-20611061 None:intergenic 15.0%
!!! TTATACTTTTGTGTATTACA+AGG + chr5_3:20611063-20611082 Msa0804670:intron 20.0%
!! AACAAATTCTAAATTTAGCA+AGG - chr5_3:20611236-20611255 None:intergenic 20.0%
!!! TTATACTTTTGTGTATTACA+AGG + chr5_3:20611063-20611082 Msa0804670:intron 20.0%
!! AACAAATTCTAAATTTAGCA+AGG - chr5_3:20611236-20611255 None:intergenic 20.0%
! ACTATTATTGTAAGGAAGAA+TGG + chr5_3:20611113-20611132 Msa0804670:intron 25.0%
! AATGGTTAAATATGTTGATG+TGG + chr5_3:20611131-20611150 Msa0804670:intron 25.0%
! TAAATTTAGCAAGGTTGTTT+AGG - chr5_3:20611227-20611246 None:intergenic 25.0%
!!! ATTTGTTATTGATTGACAGA+AGG + chr5_3:20611249-20611268 Msa0804670:intron 25.0%
! ACTATTATTGTAAGGAAGAA+TGG + chr5_3:20611113-20611132 Msa0804670:intron 25.0%
! AATGGTTAAATATGTTGATG+TGG + chr5_3:20611131-20611150 Msa0804670:intron 25.0%
! TAAATTTAGCAAGGTTGTTT+AGG - chr5_3:20611227-20611246 None:intergenic 25.0%
!!! ATTTGTTATTGATTGACAGA+AGG + chr5_3:20611249-20611268 Msa0804670:intron 25.0%
CATGTTCGAAATTACTGTAA+CGG + chr5_3:20610791-20610810 Msa0804670:CDS 30.0%
ACACTAACTTTGATCAAGTT+GGG - chr5_3:20611001-20611020 None:intergenic 30.0%
! TTTGGAAAAGTGTAATGATG+AGG + chr5_3:20611402-20611421 Msa0804670:CDS 30.0%
AATGAAAGCCAAAGTTGTTA+AGG + chr5_3:20611501-20611520 Msa0804670:CDS 30.0%
!!! ATAAGCCAAGTTTTAAGATG+AGG + chr5_3:20611629-20611648 Msa0804670:CDS 30.0%
CATGTTCGAAATTACTGTAA+CGG + chr5_3:20610791-20610810 Msa0804670:CDS 30.0%
ACACTAACTTTGATCAAGTT+GGG - chr5_3:20611001-20611020 None:intergenic 30.0%
! TTTGGAAAAGTGTAATGATG+AGG + chr5_3:20611402-20611421 Msa0804670:CDS 30.0%
AATGAAAGCCAAAGTTGTTA+AGG + chr5_3:20611501-20611520 Msa0804670:CDS 30.0%
!!! ATAAGCCAAGTTTTAAGATG+AGG + chr5_3:20611629-20611648 Msa0804670:CDS 30.0%
TATCTCGAAGCAAAGATCAA+AGG + chr5_3:20610877-20610896 Msa0804670:CDS 35.0%
! CACTAACTTTGATCAAGTTG+GGG - chr5_3:20611000-20611019 None:intergenic 35.0%
GACACTAACTTTGATCAAGT+TGG - chr5_3:20611002-20611021 None:intergenic 35.0%
TCGCGATCACTATTATTGTA+AGG + chr5_3:20611105-20611124 Msa0804670:intron 35.0%
! TATTGATTGACAGAAGGACA+AGG + chr5_3:20611255-20611274 Msa0804670:intron 35.0%
! GAAAAGTGTAATGATGAGGA+GGG + chr5_3:20611406-20611425 Msa0804670:CDS 35.0%
GATATAGATGACATCAATCC+TGG - chr5_3:20611466-20611485 None:intergenic 35.0%
CATCTCAACCTTAACAACTT+TGG - chr5_3:20611512-20611531 None:intergenic 35.0%
CAAAGTTGTTAAGGTTGAGA+TGG + chr5_3:20611510-20611529 Msa0804670:CDS 35.0%
! AAGAACCAGAAGTGTGTTAT+GGG + chr5_3:20611546-20611565 Msa0804670:CDS 35.0%
! AACCTTCATTTTCAACACCT+TGG - chr5_3:20611573-20611592 None:intergenic 35.0%
!! AGGTGTTGAAAATGAAGGTT+TGG + chr5_3:20611573-20611592 Msa0804670:CDS 35.0%
! GGTGTTGAAAATGAAGGTTT+GGG + chr5_3:20611574-20611593 Msa0804670:CDS 35.0%
TATCTCGAAGCAAAGATCAA+AGG + chr5_3:20610877-20610896 Msa0804670:CDS 35.0%
! CACTAACTTTGATCAAGTTG+GGG - chr5_3:20611000-20611019 None:intergenic 35.0%
GACACTAACTTTGATCAAGT+TGG - chr5_3:20611002-20611021 None:intergenic 35.0%
TCGCGATCACTATTATTGTA+AGG + chr5_3:20611105-20611124 Msa0804670:intron 35.0%
! TATTGATTGACAGAAGGACA+AGG + chr5_3:20611255-20611274 Msa0804670:intron 35.0%
! GAAAAGTGTAATGATGAGGA+GGG + chr5_3:20611406-20611425 Msa0804670:CDS 35.0%
GATATAGATGACATCAATCC+TGG - chr5_3:20611466-20611485 None:intergenic 35.0%
CATCTCAACCTTAACAACTT+TGG - chr5_3:20611512-20611531 None:intergenic 35.0%
CAAAGTTGTTAAGGTTGAGA+TGG + chr5_3:20611510-20611529 Msa0804670:CDS 35.0%
! AAGAACCAGAAGTGTGTTAT+GGG + chr5_3:20611546-20611565 Msa0804670:CDS 35.0%
! AACCTTCATTTTCAACACCT+TGG - chr5_3:20611573-20611592 None:intergenic 35.0%
!! AGGTGTTGAAAATGAAGGTT+TGG + chr5_3:20611573-20611592 Msa0804670:CDS 35.0%
! GGTGTTGAAAATGAAGGTTT+GGG + chr5_3:20611574-20611593 Msa0804670:CDS 35.0%
AGACGTAGAATGCGAATCAA+TGG + chr5_3:20610947-20610966 Msa0804670:CDS 40.0%
!! TAACTTTGATCAAGTTGGGG+AGG - chr5_3:20610997-20611016 None:intergenic 40.0%
TCACGATGCAGTCTAAATCA+TGG + chr5_3:20611166-20611185 Msa0804670:intron 40.0%
GGAGACTATCAAACAAGTGA+AGG + chr5_3:20611291-20611310 Msa0804670:CDS 40.0%
TGGGGCAGACAAATTGAATT+TGG + chr5_3:20611384-20611403 Msa0804670:CDS 40.0%
! GGAAAAGTGTAATGATGAGG+AGG + chr5_3:20611405-20611424 Msa0804670:CDS 40.0%
! GAAAGCTACATTGAGTTGTG+AGG + chr5_3:20611435-20611454 Msa0804670:CDS 40.0%
! GAAGAACCAGAAGTGTGTTA+TGG + chr5_3:20611545-20611564 Msa0804670:CDS 40.0%
!! GTCCAAGGTGTTGAAAATGA+AGG + chr5_3:20611568-20611587 Msa0804670:CDS 40.0%
!!! AGCCAAGTTTTAAGATGAGG+CGG + chr5_3:20611632-20611651 Msa0804670:CDS 40.0%
TACCGCCTCATCTTAAAACT+TGG - chr5_3:20611637-20611656 None:intergenic 40.0%
AGACGTAGAATGCGAATCAA+TGG + chr5_3:20610947-20610966 Msa0804670:CDS 40.0%
!! TAACTTTGATCAAGTTGGGG+AGG - chr5_3:20610997-20611016 None:intergenic 40.0%
TCACGATGCAGTCTAAATCA+TGG + chr5_3:20611166-20611185 Msa0804670:intron 40.0%
GGAGACTATCAAACAAGTGA+AGG + chr5_3:20611291-20611310 Msa0804670:CDS 40.0%
TGGGGCAGACAAATTGAATT+TGG + chr5_3:20611384-20611403 Msa0804670:CDS 40.0%
! GGAAAAGTGTAATGATGAGG+AGG + chr5_3:20611405-20611424 Msa0804670:CDS 40.0%
! GAAAGCTACATTGAGTTGTG+AGG + chr5_3:20611435-20611454 Msa0804670:CDS 40.0%
! GAAGAACCAGAAGTGTGTTA+TGG + chr5_3:20611545-20611564 Msa0804670:CDS 40.0%
!! GTCCAAGGTGTTGAAAATGA+AGG + chr5_3:20611568-20611587 Msa0804670:CDS 40.0%
!!! AGCCAAGTTTTAAGATGAGG+CGG + chr5_3:20611632-20611651 Msa0804670:CDS 40.0%
TACCGCCTCATCTTAAAACT+TGG - chr5_3:20611637-20611656 None:intergenic 40.0%
CTCGAAGCAAAGATCAAAGG+CGG + chr5_3:20610880-20610899 Msa0804670:CDS 45.0%
! GGACAAGGCATCAATACTAG+TGG + chr5_3:20611270-20611289 Msa0804670:CDS 45.0%
AGATAGCCATGCTACCTCAA+AGG + chr5_3:20611345-20611364 Msa0804670:CDS 45.0%
AGGAAACTGGATCTCCTTTG+AGG - chr5_3:20611362-20611381 None:intergenic 45.0%
TCAAAGGAGATCCAGTTTCC+TGG + chr5_3:20611361-20611380 Msa0804670:CDS 45.0%
! TTGAGTTGTGAGGATAGACC+AGG + chr5_3:20611445-20611464 Msa0804670:CDS 45.0%
TAAGGTTGAGATGGTGACAG+TGG + chr5_3:20611519-20611538 Msa0804670:CDS 45.0%
AAGGTTGAGATGGTGACAGT+GGG + chr5_3:20611520-20611539 Msa0804670:CDS 45.0%
TTGGACCCATAACACACTTC+TGG - chr5_3:20611554-20611573 None:intergenic 45.0%
! AGAAGTGTGTTATGGGTCCA+AGG + chr5_3:20611553-20611572 Msa0804670:CDS 45.0%
CTCGAAGCAAAGATCAAAGG+CGG + chr5_3:20610880-20610899 Msa0804670:CDS 45.0%
! GGACAAGGCATCAATACTAG+TGG + chr5_3:20611270-20611289 Msa0804670:CDS 45.0%
AGATAGCCATGCTACCTCAA+AGG + chr5_3:20611345-20611364 Msa0804670:CDS 45.0%
AGGAAACTGGATCTCCTTTG+AGG - chr5_3:20611362-20611381 None:intergenic 45.0%
TCAAAGGAGATCCAGTTTCC+TGG + chr5_3:20611361-20611380 Msa0804670:CDS 45.0%
! TTGAGTTGTGAGGATAGACC+AGG + chr5_3:20611445-20611464 Msa0804670:CDS 45.0%
TAAGGTTGAGATGGTGACAG+TGG + chr5_3:20611519-20611538 Msa0804670:CDS 45.0%
AAGGTTGAGATGGTGACAGT+GGG + chr5_3:20611520-20611539 Msa0804670:CDS 45.0%
TTGGACCCATAACACACTTC+TGG - chr5_3:20611554-20611573 None:intergenic 45.0%
! AGAAGTGTGTTATGGGTCCA+AGG + chr5_3:20611553-20611572 Msa0804670:CDS 45.0%
!! TCAAGTTGGGGAGGATACTG+CGG - chr5_3:20610988-20611007 None:intergenic 50.0%
!! GGATCTCCTTTGAGGTAGCA+TGG - chr5_3:20611354-20611373 None:intergenic 50.0%
AAGGAGATCCAGTTTCCTGG+TGG + chr5_3:20611364-20611383 Msa0804670:CDS 50.0%
AGGAGATCCAGTTTCCTGGT+GGG + chr5_3:20611365-20611384 Msa0804670:CDS 50.0%
TTCAATTTGTCTGCCCCACC+AGG - chr5_3:20611382-20611401 None:intergenic 50.0%
!! TCAAGTTGGGGAGGATACTG+CGG - chr5_3:20610988-20611007 None:intergenic 50.0%
!! GGATCTCCTTTGAGGTAGCA+TGG - chr5_3:20611354-20611373 None:intergenic 50.0%
AAGGAGATCCAGTTTCCTGG+TGG + chr5_3:20611364-20611383 Msa0804670:CDS 50.0%
AGGAGATCCAGTTTCCTGGT+GGG + chr5_3:20611365-20611384 Msa0804670:CDS 50.0%
TTCAATTTGTCTGCCCCACC+AGG - chr5_3:20611382-20611401 None:intergenic 50.0%
GGAGATCCAGTTTCCTGGTG+GGG + chr5_3:20611366-20611385 Msa0804670:CDS 55.0%
GTTGAGATGGTGACAGTGGG+TGG + chr5_3:20611523-20611542 Msa0804670:CDS 55.0%
GGAGATCCAGTTTCCTGGTG+GGG + chr5_3:20611366-20611385 Msa0804670:CDS 55.0%
GTTGAGATGGTGACAGTGGG+TGG + chr5_3:20611523-20611542 Msa0804670:CDS 55.0%
GTTGGGGAGGATACTGCGGA+GGG - chr5_3:20610984-20611003 None:intergenic 60.0%
AGTTGGGGAGGATACTGCGG+AGG - chr5_3:20610985-20611004 None:intergenic 60.0%
TGTCTGCCCCACCAGGAAAC+TGG - chr5_3:20611375-20611394 None:intergenic 60.0%
GTTGGGGAGGATACTGCGGA+GGG - chr5_3:20610984-20611003 None:intergenic 60.0%
AGTTGGGGAGGATACTGCGG+AGG - chr5_3:20610985-20611004 None:intergenic 60.0%
TGTCTGCCCCACCAGGAAAC+TGG - chr5_3:20611375-20611394 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 20610785 20611666 20610785 ID=Msa0804670;Name=Msa0804670
chr5_3 mRNA 20610785 20611666 20610785 ID=Msa0804670-mRNA-1;Parent=Msa0804670;Name=Msa0804670-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|208
chr5_3 exon 20610785 20611012 20610785 ID=Msa0804670-mRNA-1:exon:8321;Parent=Msa0804670-mRNA-1
chr5_3 exon 20611268 20611666 20611268 ID=Msa0804670-mRNA-1:exon:8322;Parent=Msa0804670-mRNA-1
chr5_3 CDS 20610785 20611012 20610785 ID=Msa0804670-mRNA-1:cds;Parent=Msa0804670-mRNA-1
chr5_3 CDS 20611268 20611666 20611268 ID=Msa0804670-mRNA-1:cds;Parent=Msa0804670-mRNA-1
Gene Sequence

>Msa0804670

ATGCAGCATGTTCGAAATTACTGTAACGGAGAAGCTCAAATGCTAAGAAGCAACTTTTCAAGTTCATTCATCTCGCAAACATTCAAAAACAGTATCTCGAAGCAAAGATCAAAGGCGGAGAACAAATTACTAGCAGCTAAGAAACATAGCGAAGCAGAAAAGAGACGTAGAATGCGAATCAATGGTCAATATGACACCCTCCGCAGTATCCTCCCCAACTTGATCAAAAAGGACAAGGCATCAATACTAGTGGAGACTATCAAACAAGTGAAGGAGTTGAAGAAAAAAGCATCAAAACTAGAAGAAGATAGCCATGCTACCTCAAAGGAGATCCAGTTTCCTGGTGGGGCAGACAAATTGAATTTGGAAAAGTGTAATGATGAGGAGGGTCTAGTGAAAGCTACATTGAGTTGTGAGGATAGACCAGGATTGATGTCATCTATATCAAGAGCACTATTGTCAATGAAAGCCAAAGTTGTTAAGGTTGAGATGGTGACAGTGGGTGGAAGAACCAGAAGTGTGTTATGGGTCCAAGGTGTTGAAAATGAAGGTTTGGGAATGCTCAAGAGTACTTTGAAAGTTGTTATGCATAAGCCAAGTTTTAAGATGAGGCGGTATACTCAATAG

Protein sequence

>Msa0804670

MQHVRNYCNGEAQMLRSNFSSSFISQTFKNSISKQRSKAENKLLAAKKHSEAEKRRRMRINGQYDTLRSILPNLIKKDKASILVETIKQVKELKKKASKLEEDSHATSKEIQFPGGADKLNLEKCNDEEGLVKATLSCEDRPGLMSSISRALLSMKAKVVKVEMVTVGGRTRSVLWVQGVENEGLGMLKSTLKVVMHKPSFKMRRYTQ*