AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0807660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0807660 MtrunA17_Chr2g0296781 74.198 686 175 2 43 728 1 684 0.0 1060
Msa0807660 MtrunA17_Chr1g0196541 64.731 689 236 2 43 731 1 682 0.0 912
Msa0807660 MtrunA17_Chr6g0477261 76.481 574 134 1 155 728 1 573 0.0 912
Msa0807660 MtrunA17_Chr5g0441581 47.419 620 309 6 33 646 21 629 0.0 572
Msa0807660 MtrunA17_Chr2g0291581 37.119 590 351 9 17 600 47 622 2.36e-129 400
Msa0807660 MtrunA17_Chr1g0207551 36.907 569 348 5 36 601 31 591 1.13e-125 394
Msa0807660 MtrunA17_Chr5g0448761 36.968 587 345 10 33 604 54 630 5.70e-123 386
Msa0807660 MtrunA17_Chr1g0202341 37.108 574 337 11 38 600 50 610 2.00e-119 374
Msa0807660 MtrunA17_Chr2g0329031 35.862 580 347 10 31 604 6 566 6.55e-119 374
Msa0807660 MtrunA17_Chr3g0080351 35.775 587 328 9 33 601 70 625 3.89e-117 372
Msa0807660 MtrunA17_Chr2g0291591 34.476 525 338 4 12 533 37 558 5.80e-106 335
Msa0807660 MtrunA17_Chr5g0440451 33.008 615 359 15 38 625 42 630 2.46e-94 314
Msa0807660 MtrunA17_Chr5g0448511 36.700 406 245 5 197 599 1 397 1.58e-87 286
Msa0807660 MtrunA17_Chr3g0118611 37.772 413 243 5 209 620 118 517 6.46e-86 288
Msa0807660 MtrunA17_Chr2g0287651 27.832 618 429 7 43 653 79 686 3.53e-84 285
Msa0807660 MtrunA17_Chr2g0324011 29.534 579 376 10 36 599 7 568 1.05e-80 269
Msa0807660 MtrunA17_Chr2g0287061 31.217 567 334 12 38 597 182 699 2.12e-79 270
Msa0807660 MtrunA17_Chr8g0363841 30.479 584 371 13 35 599 10 577 7.54e-77 261
Msa0807660 MtrunA17_Chr2g0276841 28.111 683 426 18 16 665 47 697 4.46e-75 256
Msa0807660 MtrunA17_Chr6g0476441 67.368 190 61 1 539 728 1 189 9.02e-74 241
Msa0807660 MtrunA17_Chr1g0198761 28.183 699 425 23 42 701 50 710 1.32e-69 243
Msa0807660 MtrunA17_Chr6g0458391 28.152 579 383 11 28 599 213 765 2.32e-69 242
Msa0807660 MtrunA17_Chr1g0203051 27.759 598 381 15 10 580 43 616 6.67e-67 233
Msa0807660 MtrunA17_Chr1g0203721 28.970 573 379 11 41 599 65 623 7.01e-66 234
Msa0807660 MtrunA17_Chr3g0082121 28.258 729 447 22 18 720 17 695 2.48e-65 230
Msa0807660 MtrunA17_Chr2g0294841 26.307 574 390 11 39 594 45 603 2.66e-58 211
Msa0807660 MtrunA17_Chr2g0290391 26.016 615 397 17 13 597 25 611 2.43e-50 187
Msa0807660 MtrunA17_Chr5g0431001 32.390 318 204 5 132 443 168 480 1.19e-49 182
Msa0807660 MtrunA17_Chr1g0164591 32.698 315 188 7 121 427 10 308 8.01e-46 166
Msa0807660 MtrunA17_Chr7g0258861 32.753 287 187 3 32 312 3 289 2.15e-45 164
Msa0807660 MtrunA17_Chr6g0476431 59.302 86 35 0 43 128 1 86 1.45e-29 112
Msa0807660 MtrunA17_Chr2g0308841 24.675 231 159 3 371 596 4 224 4.46e-19 87.8
Msa0807660 MtrunA17_Chr6g0451441 31.183 186 112 5 29 201 65 247 4.80e-19 90.9
Msa0807660 MtrunA17_Chr1g0172041 33.636 110 72 1 511 620 1 109 2.95e-14 69.7
Msa0807660 MtrunA17_Chr3g0077731 57.143 77 32 1 652 728 1 76 5.62e-13 66.2
Msa0807660 MtrunA17_Chr2g0329361 33.043 115 61 3 38 141 60 169 9.34e-12 65.9
Msa0807660 MtrunA17_Chr1g0149131 30.000 110 76 1 314 422 1 110 5.30e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0807660 AT4G15090.1 34.342 661 416 10 21 674 33 682 4.93e-129 404
Msa0807660 AT4G15090.5 34.342 661 416 10 21 674 33 682 4.93e-129 404
Msa0807660 AT4G15090.3 34.342 661 416 10 21 674 33 682 4.93e-129 404
Msa0807660 AT4G15090.2 34.342 661 416 10 21 674 33 682 4.93e-129 404
Msa0807660 AT4G15090.4 34.342 661 416 10 21 674 33 682 4.93e-129 404
Msa0807660 AT4G38180.1 35.052 582 363 8 26 599 60 634 2.47e-117 372
Msa0807660 AT1G52520.1 34.725 599 367 13 11 600 56 639 8.13e-116 365
Msa0807660 AT3G22170.2 35.484 589 332 9 32 602 63 621 3.08e-115 367
Msa0807660 AT3G22170.1 35.484 589 332 9 32 602 63 621 3.08e-115 367
Msa0807660 AT1G76320.3 35.979 567 340 9 43 604 1 549 1.33e-111 353
Msa0807660 AT1G76320.2 35.789 570 343 9 43 607 1 552 3.02e-111 354
Msa0807660 AT1G76320.1 35.789 570 343 9 43 607 1 552 3.72e-111 354
Msa0807660 AT1G76320.4 35.789 570 343 9 43 607 1 552 3.72e-111 354
Msa0807660 AT1G80010.1 33.777 601 362 16 26 603 78 665 5.41e-103 332
Msa0807660 AT4G19990.5 32.660 594 316 10 42 604 24 564 1.44e-93 308
Msa0807660 AT4G19990.3 32.660 594 316 10 42 604 24 564 1.65e-93 308
Msa0807660 AT4G19990.4 32.660 594 316 10 42 604 24 564 1.65e-93 308
Msa0807660 AT4G19990.7 32.660 594 316 10 42 604 38 578 2.61e-93 308
Msa0807660 AT4G19990.6 32.660 594 316 10 42 604 24 564 3.13e-93 308
Msa0807660 AT4G19990.2 32.660 594 316 10 42 604 24 564 3.13e-93 308
Msa0807660 AT2G27110.2 31.757 592 368 8 13 599 19 579 3.29e-91 304
Msa0807660 AT2G27110.1 31.757 592 368 8 13 599 19 579 3.29e-91 304
Msa0807660 AT4G19990.1 33.766 539 301 9 69 604 22 507 7.00e-91 299
Msa0807660 AT2G32250.5 32.562 562 328 8 38 596 37 550 1.15e-89 298
Msa0807660 AT2G32250.2 32.562 562 328 8 38 596 37 550 2.47e-89 298
Msa0807660 AT2G32250.4 32.562 562 328 8 38 596 37 550 2.47e-89 298
Msa0807660 AT2G32250.6 32.562 562 328 8 38 596 37 550 3.58e-89 297
Msa0807660 AT2G32250.1 32.562 562 328 8 38 596 37 550 3.58e-89 297
Msa0807660 AT2G32250.3 32.562 562 328 8 38 596 37 550 3.58e-89 297
Msa0807660 AT2G32250.7 32.496 557 325 8 43 596 1 509 1.45e-88 295
Msa0807660 AT2G32250.8 32.496 557 325 8 43 596 1 509 2.43e-88 294
Msa0807660 AT2G27110.3 34.325 437 270 5 164 599 14 434 1.23e-81 275
Msa0807660 AT3G06250.2 30.849 577 337 13 29 597 180 702 9.10e-81 274
Msa0807660 AT3G06250.1 30.849 577 337 13 29 597 180 702 9.10e-81 274
Msa0807660 AT3G06250.3 30.849 577 337 13 29 597 180 702 9.10e-81 274
Msa0807660 AT5G18960.2 31.217 567 333 14 38 597 210 726 1.81e-77 265
Msa0807660 AT5G18960.1 31.217 567 333 14 38 597 210 726 1.81e-77 265
Msa0807660 AT4G38170.1 34.684 395 246 5 211 603 19 403 1.42e-75 254
Msa0807660 AT1G10240.2 27.524 614 384 17 9 594 22 602 2.52e-65 230
Msa0807660 AT1G10240.1 27.524 614 384 17 9 594 22 602 2.52e-65 230
Msa0807660 AT5G28530.3 25.463 648 417 19 39 651 55 671 1.14e-49 186
Msa0807660 AT5G28530.2 25.463 648 417 19 39 651 55 671 1.14e-49 186
Msa0807660 AT5G28530.1 25.460 652 416 20 39 651 55 675 8.93e-49 183
Msa0807660 AT5G28530.5 23.354 638 380 25 39 651 55 608 1.85e-28 121
Msa0807660 AT5G28530.4 23.364 642 379 26 39 651 55 612 9.73e-28 119

Find 99 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTGTTTATTTGAGTTTA+AGG 0.151620 5_3:+30529749 Msa0807660:CDS
ATTGCTTGTATAAGTCATTT+TGG 0.199496 5_3:+30529510 Msa0807660:CDS
TCCATCCTGGTCAGTAATTA+TGG 0.258712 5_3:-30529037 None:intergenic
TATGCAAGATGTATAGGTTT+TGG 0.261658 5_3:+30528247 Msa0807660:CDS
TTTGAGCATAAGTGATTTCA+TGG 0.262311 5_3:+30529209 Msa0807660:CDS
AGGGCTGCTTATGAGTATTT+TGG 0.265157 5_3:+30528823 Msa0807660:CDS
TGTATGTGAGAGACACATTT+TGG 0.270477 5_3:+30529322 Msa0807660:CDS
TTGCATTGGTATATGCCTTT+TGG 0.297420 5_3:-30529548 None:intergenic
CACCACCGATGGCGAGCTTT+CGG 0.299987 5_3:-30529090 None:intergenic
TAAAATTGAAAAGGAAAATA+TGG 0.301034 5_3:+30529461 Msa0807660:CDS
TGGAACAACTACTATTTCTA+AGG 0.304844 5_3:+30528435 Msa0807660:CDS
TATACAAGCAATAATTCTAT+TGG 0.306437 5_3:-30529499 None:intergenic
AAGAATTATGCAAGATGTAT+AGG 0.307421 5_3:+30528241 Msa0807660:CDS
TAGTATCCTCCTTCGACAAT+AGG 0.312589 5_3:-30528954 None:intergenic
TGAAGAAGCAACTTCGTAAA+AGG 0.325276 5_3:-30529955 None:intergenic
TGTTGTTTATTTGAGTTTAA+GGG 0.345986 5_3:+30529750 Msa0807660:CDS
ACGCTGCAACTCTTCATTTC+CGG 0.353530 5_3:-30529913 None:intergenic
TTTCTTGGTTGTTCACTACT+TGG 0.357896 5_3:+30528983 Msa0807660:CDS
CTAAAATATCTTGATTTGCT+TGG 0.361295 5_3:-30528490 None:intergenic
TCATGCTTTCTTTGAGAGAA+TGG 0.375982 5_3:+30529614 Msa0807660:CDS
TTGTGTGACAGAAAGTAAAA+AGG 0.380761 5_3:+30529656 Msa0807660:CDS
TTATTGACCCTAGTATAAAA+AGG 0.385981 5_3:+30528524 Msa0807660:CDS
CTTGTGGCATATAATGAAAA+AGG 0.386165 5_3:+30529113 Msa0807660:CDS
TAGAATAGAGAACTTAAAAC+TGG 0.390920 5_3:+30530016 Msa0807660:CDS
CATGGTCAATCAGTTCTGTT+AGG 0.391566 5_3:+30528925 Msa0807660:CDS
AGTATCCTCCTTCGACAATA+GGG 0.392582 5_3:-30528953 None:intergenic
ACCACCGATGGCGAGCTTTC+GGG 0.394899 5_3:-30529089 None:intergenic
GTTATCTTTGATATTATGTC+TGG 0.397655 5_3:+30527513 None:intergenic
ATTGTGAAAAGGAAGCTTGC+TGG 0.406491 5_3:+30530182 Msa0807660:CDS
GGATATGATAACCTTACATT+CGG 0.409109 5_3:+30528619 Msa0807660:CDS
CCTTTACTTCGAGTCGCTAC+AGG 0.409218 5_3:-30530119 None:intergenic
AAGGGCGTCCTCCTTCAAAA+AGG 0.415773 5_3:+30530138 Msa0807660:CDS
AGGAAGCTTGCTGGAAAGAA+AGG 0.424206 5_3:+30530191 Msa0807660:CDS
TTAAATTTCATGCCAACCTT+AGG 0.424975 5_3:-30528190 None:intergenic
GCTGGAAAGAAAGGACAAAA+AGG 0.427372 5_3:+30530200 Msa0807660:CDS
GTTTGATGAGCGTCATCGTT+GGG 0.431278 5_3:+30529293 Msa0807660:CDS
AAGTGAGGCGGCTTCGACTT+GGG 0.432744 5_3:+30528671 Msa0807660:CDS
ATATTTATTTGTTAAGTAAG+TGG 0.436838 5_3:-30528866 None:intergenic
CATTCTTCATTGCTCCATCC+TGG 0.449843 5_3:-30529050 None:intergenic
CATGATAATGAATGGTTGAA+AGG 0.452805 5_3:+30529267 Msa0807660:CDS
CTTGTTATATATTGCCAAGA+TGG 0.455125 5_3:+30529835 Msa0807660:CDS
ACTTCAACAAAAGGAGCAAA+AGG 0.456764 5_3:-30528895 None:intergenic
GATATGATAACCTTACATTC+GGG 0.457280 5_3:+30528620 Msa0807660:CDS
TTGTTATATATTGCCAAGAT+GGG 0.466035 5_3:+30529836 Msa0807660:CDS
CTCGCCATCGGTGGTGCTTG+TGG 0.467564 5_3:+30529097 Msa0807660:CDS
TGATGATTTACTTCAACAAA+AGG 0.471065 5_3:-30528904 None:intergenic
CAAAGAGTTTCAAGTAGAAG+TGG 0.481571 5_3:+30529575 Msa0807660:CDS
AGATATACACTTATTAAAAG+TGG 0.483850 5_3:+30529876 Msa0807660:CDS
TTTGGGCAGATGCGAGATGT+AGG 0.485083 5_3:+30528803 Msa0807660:CDS
TACTTACATTAATCCCAGCT+TGG 0.488151 5_3:-30528564 None:intergenic
CCTGTAGCGACTCGAAGTAA+AGG 0.488591 5_3:+30530119 Msa0807660:CDS
TAGCATGAAAACACACTGTA+AGG 0.490277 5_3:+30528384 Msa0807660:CDS
AGTTAAAGACATAATGTTCA+AGG 0.493487 5_3:+30529689 Msa0807660:CDS
ATTTGAATCTCAATTCCAAA+AGG 0.494664 5_3:+30529533 Msa0807660:CDS
CTGTAGCGACTCGAAGTAAA+GGG 0.498287 5_3:+30530120 Msa0807660:CDS
ATTGAAAGATAAAATTGAAA+AGG 0.501824 5_3:+30529452 Msa0807660:CDS
TTTGTTGAAGTAAATCATCA+TGG 0.506547 5_3:+30528907 Msa0807660:CDS
AGGCGGCTTCGACTTGGGAC+AGG 0.507987 5_3:+30528676 Msa0807660:CDS
TTGGGCAGATGCGAGATGTA+GGG 0.512318 5_3:+30528804 Msa0807660:CDS
TGTTTGATGAGCGTCATCGT+TGG 0.518510 5_3:+30529292 Msa0807660:CDS
AAAGTGAGGCGGCTTCGACT+TGG 0.518819 5_3:+30528670 Msa0807660:CDS
AAATGATTTATCGAAAGTAA+TGG 0.527562 5_3:+30529992 Msa0807660:CDS
TGTTAAGTAAGTGGTATCAA+AGG 0.528179 5_3:-30528857 None:intergenic
TTGGAGGTTAATGACCAAGC+TGG 0.528235 5_3:+30528550 Msa0807660:CDS
ATGATAATGAATGGTTGAAA+GGG 0.531139 5_3:+30529268 Msa0807660:CDS
ACCCGAAAGCTCGCCATCGG+TGG 0.532685 5_3:+30529088 Msa0807660:CDS
TGGAGGTTAATGACCAAGCT+GGG 0.539674 5_3:+30528551 Msa0807660:CDS
TACTCACATAGCTTCTTGCA+CGG 0.540398 5_3:-30528333 None:intergenic
ATGAACTTCATGATAATGAA+TGG 0.543870 5_3:+30529259 Msa0807660:CDS
AAATGAAGATGATGAACCTA+AGG 0.544931 5_3:+30528174 Msa0807660:CDS
AAGTTGATCAAATTGTGAAA+AGG 0.546898 5_3:+30530171 Msa0807660:CDS
AAGTGATTTCATGGAGAAGT+GGG 0.549527 5_3:+30529218 Msa0807660:CDS
GAAGATGATGAACCTAAGGT+TGG 0.554642 5_3:+30528178 Msa0807660:CDS
TAAGTGATTTCATGGAGAAG+TGG 0.562788 5_3:+30529217 Msa0807660:CDS
AATGTATGTGTGTCTTCAGA+TGG 0.571681 5_3:+30528415 Msa0807660:CDS
TGTTATATATTGCCAAGATG+GGG 0.574247 5_3:+30529837 Msa0807660:CDS
TGGTCAGTAATTATGGCTTG+TGG 0.579259 5_3:-30529030 None:intergenic
TCTACCCGAAAGCTCGCCAT+CGG 0.579533 5_3:+30529085 Msa0807660:CDS
TGCTTTCTTTGAGAGAATGG+AGG 0.581817 5_3:+30529617 Msa0807660:CDS
ATGGCGAGCTTTCGGGTAGA+CGG 0.587359 5_3:-30529082 None:intergenic
CATGAAATTTAATTCTGCAG+AGG 0.587606 5_3:+30528201 Msa0807660:CDS
AGGTTGCGCCCTATTGTCGA+AGG 0.587751 5_3:+30528945 Msa0807660:CDS
CACTTATCAAAGTCAAGAAG+AGG 0.608354 5_3:+30530238 Msa0807660:CDS
GAAACTACATTGACAAAGTG+AGG 0.624787 5_3:+30528656 Msa0807660:CDS
GTTATATATTGCCAAGATGG+GGG 0.625320 5_3:+30529838 Msa0807660:CDS
ACAAGCCATAATTACTGACC+AGG 0.626330 5_3:+30529032 Msa0807660:CDS
AGCAATGAAGAATGCAGTTG+AGG 0.631682 5_3:+30529059 Msa0807660:CDS
AATGTGTCTCTCACATACAC+AGG 0.631924 5_3:-30529318 None:intergenic
TCTTGGCAATATATAACAAG+AGG 0.632035 5_3:-30529832 None:intergenic
GCCATAATTACTGACCAGGA+TGG 0.636449 5_3:+30529036 Msa0807660:CDS
TCTATGGTTGTTGAAGCAAG+TGG 0.641961 5_3:+30528598 Msa0807660:CDS
CTTGGCAATATATAACAAGA+GGG 0.647143 5_3:-30529831 None:intergenic
TTGCGCCCTATTGTCGAAGG+AGG 0.658239 5_3:+30528948 Msa0807660:CDS
TCACATAAACGAAAATTGTG+AGG 0.663983 5_3:+30528138 Msa0807660:CDS
TATGCCACAAGCACCACCGA+TGG 0.685228 5_3:-30529101 None:intergenic
CTGTGAGCTTAAGAACACAA+AGG 0.694006 5_3:-30529791 None:intergenic
ATATGATAACCTTACATTCG+GGG 0.715139 5_3:+30528621 Msa0807660:CDS
ACTTGGTTAGAATGTATGCG+TGG 0.724733 5_3:+30529000 Msa0807660:CDS
ACTACATTGACAAAGTGAGG+CGG 0.739143 5_3:+30528659 Msa0807660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAAAAATTATATAAAA+AGG - chr5_3:30527656-30527675 None:intergenic 0.0%
!! AAAATAAATAAATAAATATA+TGG + chr5_3:30527684-30527703 Msa0807660:intron 0.0%
!! AAATTATATAAAAAGGAAAT+TGG - chr5_3:30527649-30527668 None:intergenic 10.0%
!!! TAATGAATTTTATTATGTAA+TGG + chr5_3:30528747-30528766 Msa0807660:CDS 10.0%
!!! TTAATGTAAGTAGAAATTTT+CGG + chr5_3:30528575-30528594 Msa0807660:CDS 15.0%
!!! TCTAACAAAATAATTTTGAA+TGG - chr5_3:30528713-30528732 None:intergenic 15.0%
!!! ATATTTATTTGTTAAGTAAG+TGG - chr5_3:30528869-30528888 None:intergenic 15.0%
!! ATTGAAAGATAAAATTGAAA+AGG + chr5_3:30529452-30529471 Msa0807660:CDS 15.0%
!! TAAAATTGAAAAGGAAAATA+TGG + chr5_3:30529461-30529480 Msa0807660:CDS 15.0%
!! TATAAATAGAATTCAGATCT+TGG + chr5_3:30527547-30527566 Msa0807660:intron 20.0%
!! TATATAAAAAGGAAATTGGA+TGG - chr5_3:30527645-30527664 None:intergenic 20.0%
!! ATCAACTGAATTAAAAGTAA+AGG - chr5_3:30528025-30528044 None:intergenic 20.0%
!!! TAAAAAATTTGTTGCATTAG+TGG + chr5_3:30528063-30528082 Msa0807660:intron 20.0%
!!! GAAAAAAGTATTTTACTCTA+GGG + chr5_3:30528305-30528324 Msa0807660:CDS 20.0%
!!! ATTTGGCTTTAAAAGATTTT+TGG - chr5_3:30528362-30528381 None:intergenic 20.0%
!!! ATTTTATTATGTAATGGATG+TGG + chr5_3:30528753-30528772 Msa0807660:CDS 20.0%
!! TATACAAGCAATAATTCTAT+TGG - chr5_3:30529502-30529521 None:intergenic 20.0%
!!! ATGTTGTTTATTTGAGTTTA+AGG + chr5_3:30529749-30529768 Msa0807660:CDS 20.0%
!!! TGTTGTTTATTTGAGTTTAA+GGG + chr5_3:30529750-30529769 Msa0807660:CDS 20.0%
!! AGATATACACTTATTAAAAG+TGG + chr5_3:30529876-30529895 Msa0807660:CDS 20.0%
!! AAATGATTTATCGAAAGTAA+TGG + chr5_3:30529992-30530011 Msa0807660:CDS 20.0%
!!! ATTTTGATTTTGGACAATCA+CGG + chr5_3:30527727-30527746 Msa0807660:intron 25.0%
! AATCAGAACATATAAGGTTA+TGG - chr5_3:30527768-30527787 None:intergenic 25.0%
! GAACAAAATCAGAACATATA+AGG - chr5_3:30527774-30527793 None:intergenic 25.0%
! GGAAATCAAATTTCAATTGA+TGG - chr5_3:30527923-30527942 None:intergenic 25.0%
! AAGAATTATGCAAGATGTAT+AGG + chr5_3:30528241-30528260 Msa0807660:CDS 25.0%
! TAGCATAATCAGTACAAAAA+AGG + chr5_3:30528273-30528292 Msa0807660:CDS 25.0%
! AGCATAATCAGTACAAAAAA+GGG + chr5_3:30528274-30528293 Msa0807660:CDS 25.0%
!!! GGAAAAAAGTATTTTACTCT+AGG + chr5_3:30528304-30528323 Msa0807660:CDS 25.0%
!!! CTAAAATATCTTGATTTGCT+TGG - chr5_3:30528493-30528512 None:intergenic 25.0%
! TTATTGACCCTAGTATAAAA+AGG + chr5_3:30528524-30528543 Msa0807660:CDS 25.0%
!!! TCGTTTACGAAATGTTTTTT+GGG + chr5_3:30528786-30528805 Msa0807660:CDS 25.0%
! TGATGATTTACTTCAACAAA+AGG - chr5_3:30528907-30528926 None:intergenic 25.0%
! TTTGTTGAAGTAAATCATCA+TGG + chr5_3:30528907-30528926 Msa0807660:CDS 25.0%
!!! AGGATACTAAAACTTTTTCT+TGG + chr5_3:30528968-30528987 Msa0807660:CDS 25.0%
!! ATGAACTTCATGATAATGAA+TGG + chr5_3:30529259-30529278 Msa0807660:CDS 25.0%
!! ATGATAATGAATGGTTGAAA+GGG + chr5_3:30529268-30529287 Msa0807660:CDS 25.0%
!! ATTGCTTGTATAAGTCATTT+TGG + chr5_3:30529510-30529529 Msa0807660:CDS 25.0%
! ATTTGAATCTCAATTCCAAA+AGG + chr5_3:30529533-30529552 Msa0807660:CDS 25.0%
!! AAACTCTTTGAATTTTGCAT+TGG - chr5_3:30529565-30529584 None:intergenic 25.0%
! AGTTAAAGACATAATGTTCA+AGG + chr5_3:30529689-30529708 Msa0807660:CDS 25.0%
! TTGTTATATATTGCCAAGAT+GGG + chr5_3:30529836-30529855 Msa0807660:CDS 25.0%
!! TCGATAAATCATTTTCAGTA+TGG - chr5_3:30529986-30530005 None:intergenic 25.0%
! TAGAATAGAGAACTTAAAAC+TGG + chr5_3:30530016-30530035 Msa0807660:CDS 25.0%
!! AAGTTGATCAAATTGTGAAA+AGG + chr5_3:30530171-30530190 Msa0807660:CDS 25.0%
TAAAAAGGAAATTGGATGGT+GGG - chr5_3:30527641-30527660 None:intergenic 30.0%
ATAAAAAGGAAATTGGATGG+TGG - chr5_3:30527642-30527661 None:intergenic 30.0%
ATATATGGAAGAGACAATCA+CGG + chr5_3:30527699-30527718 Msa0807660:intron 30.0%
AACATATAAGGTTATGGAGA+AGG - chr5_3:30527762-30527781 None:intergenic 30.0%
!!! AAATTCAGTTTTGGAATCGA+CGG - chr5_3:30527828-30527847 None:intergenic 30.0%
! CAAATTTCAATTGATGGTTG+AGG - chr5_3:30527917-30527936 None:intergenic 30.0%
TTGAACTGGATGCTAAAATA+GGG - chr5_3:30527944-30527963 None:intergenic 30.0%
TCACATAAACGAAAATTGTG+AGG + chr5_3:30528138-30528157 Msa0807660:CDS 30.0%
AAATGAAGATGATGAACCTA+AGG + chr5_3:30528174-30528193 Msa0807660:CDS 30.0%
TTAAATTTCATGCCAACCTT+AGG - chr5_3:30528193-30528212 None:intergenic 30.0%
CATGAAATTTAATTCTGCAG+AGG + chr5_3:30528201-30528220 Msa0807660:CDS 30.0%
! TATGCAAGATGTATAGGTTT+TGG + chr5_3:30528247-30528266 Msa0807660:CDS 30.0%
! TGTGTTTTCATGCTAAGATT+TGG - chr5_3:30528379-30528398 None:intergenic 30.0%
!! TGGAACAACTACTATTTCTA+AGG + chr5_3:30528435-30528454 Msa0807660:CDS 30.0%
! CCAATCTCCTTTTTATACTA+GGG - chr5_3:30528534-30528553 None:intergenic 30.0%
! TCCAATCTCCTTTTTATACT+AGG - chr5_3:30528535-30528554 None:intergenic 30.0%
TAGTATAAAAAGGAGATTGG+AGG + chr5_3:30528534-30528553 Msa0807660:CDS 30.0%
! AAGTAGAAATTTTCGGTCTA+TGG + chr5_3:30528582-30528601 Msa0807660:CDS 30.0%
GGATATGATAACCTTACATT+CGG + chr5_3:30528619-30528638 Msa0807660:CDS 30.0%
GATATGATAACCTTACATTC+GGG + chr5_3:30528620-30528639 Msa0807660:CDS 30.0%
ATATGATAACCTTACATTCG+GGG + chr5_3:30528621-30528640 Msa0807660:CDS 30.0%
!!! GTCGTTTACGAAATGTTTTT+TGG + chr5_3:30528785-30528804 Msa0807660:CDS 30.0%
TGTTAAGTAAGTGGTATCAA+AGG - chr5_3:30528860-30528879 None:intergenic 30.0%
CTTGTGGCATATAATGAAAA+AGG + chr5_3:30529113-30529132 Msa0807660:CDS 30.0%
! CAAAATACTTTTGCATGATG+CGG + chr5_3:30529176-30529195 Msa0807660:CDS 30.0%
TTTGAGCATAAGTGATTTCA+TGG + chr5_3:30529209-30529228 Msa0807660:CDS 30.0%
! CATGATAATGAATGGTTGAA+AGG + chr5_3:30529267-30529286 Msa0807660:CDS 30.0%
!!! AGTATGAACGCATTTTTTGA+TGG + chr5_3:30529375-30529394 Msa0807660:CDS 30.0%
TTGTGTGACAGAAAGTAAAA+AGG + chr5_3:30529656-30529675 Msa0807660:CDS 30.0%
CTTGGCAATATATAACAAGA+GGG - chr5_3:30529834-30529853 None:intergenic 30.0%
TCTTGGCAATATATAACAAG+AGG - chr5_3:30529835-30529854 None:intergenic 30.0%
! CTTGTTATATATTGCCAAGA+TGG + chr5_3:30529835-30529854 Msa0807660:CDS 30.0%
TGTTATATATTGCCAAGATG+GGG + chr5_3:30529837-30529856 Msa0807660:CDS 30.0%
GTTGAAACAGTTGTGCTATA+TGG + chr5_3:30527574-30527593 Msa0807660:intron 35.0%
TATTCTTTCGTGCAATTCGT+GGG - chr5_3:30527612-30527631 None:intergenic 35.0%
TTATTCTTTCGTGCAATTCG+TGG - chr5_3:30527613-30527632 None:intergenic 35.0%
AAAAAGGAAATTGGATGGTG+GGG - chr5_3:30527640-30527659 None:intergenic 35.0%
GACAATCAAACTAACAACTC+TGG - chr5_3:30527859-30527878 None:intergenic 35.0%
CTTGAACTGGATGCTAAAAT+AGG - chr5_3:30527945-30527964 None:intergenic 35.0%
CTTACTATTGTCTCTTGAAC+TGG - chr5_3:30527958-30527977 None:intergenic 35.0%
AGTACAAAAAAGGGAGATGA+TGG + chr5_3:30528283-30528302 Msa0807660:CDS 35.0%
TAGCATGAAAACACACTGTA+AGG + chr5_3:30528384-30528403 Msa0807660:CDS 35.0%
AATGTATGTGTGTCTTCAGA+TGG + chr5_3:30528415-30528434 Msa0807660:CDS 35.0%
CCCTAGTATAAAAAGGAGAT+TGG + chr5_3:30528531-30528550 Msa0807660:CDS 35.0%
TACTTACATTAATCCCAGCT+TGG - chr5_3:30528567-30528586 None:intergenic 35.0%
GAAACTACATTGACAAAGTG+AGG + chr5_3:30528656-30528675 Msa0807660:CDS 35.0%
ACTTCAACAAAAGGAGCAAA+AGG - chr5_3:30528898-30528917 None:intergenic 35.0%
TTTCTTGGTTGTTCACTACT+TGG + chr5_3:30528983-30529002 Msa0807660:CDS 35.0%
! GAGAGTCTACTCAATTTTTC+TGG - chr5_3:30529141-30529160 None:intergenic 35.0%
TAAGTGATTTCATGGAGAAG+TGG + chr5_3:30529217-30529236 Msa0807660:CDS 35.0%
AAGTGATTTCATGGAGAAGT+GGG + chr5_3:30529218-30529237 Msa0807660:CDS 35.0%
! TGTATGTGAGAGACACATTT+TGG + chr5_3:30529322-30529341 Msa0807660:CDS 35.0%
! GTATGTGAGAGACACATTTT+GGG + chr5_3:30529323-30529342 Msa0807660:CDS 35.0%
!! TTGCATTGGTATATGCCTTT+TGG - chr5_3:30529551-30529570 None:intergenic 35.0%
! CAAAGAGTTTCAAGTAGAAG+TGG + chr5_3:30529575-30529594 Msa0807660:CDS 35.0%
! TCATGCTTTCTTTGAGAGAA+TGG + chr5_3:30529614-30529633 Msa0807660:CDS 35.0%
GTTATATATTGCCAAGATGG+GGG + chr5_3:30529838-30529857 Msa0807660:CDS 35.0%
!!! AGTGGTTTTGATCAATTAGC+CGG + chr5_3:30529894-30529913 Msa0807660:CDS 35.0%
TGAAGAAGCAACTTCGTAAA+AGG - chr5_3:30529958-30529977 None:intergenic 35.0%
!! AGTTTTTCAGAGATCACTGA+AGG + chr5_3:30530056-30530075 Msa0807660:CDS 35.0%
! TTAGAAGCCTTCCTTTTTGA+AGG - chr5_3:30530152-30530171 None:intergenic 35.0%
CACTTATCAAAGTCAAGAAG+AGG + chr5_3:30530238-30530257 Msa0807660:CDS 35.0%
ATTCTTTCGTGCAATTCGTG+GGG - chr5_3:30527611-30527630 None:intergenic 40.0%
!!! CACGGCACACATTTTGATTT+TGG + chr5_3:30527717-30527736 Msa0807660:intron 40.0%
! GAAGCGACGAAATTCAGTTT+TGG - chr5_3:30527837-30527856 None:intergenic 40.0%
GTAGCAATGCTGATAAACAG+AGG - chr5_3:30527889-30527908 None:intergenic 40.0%
GAAGATGATGAACCTAAGGT+TGG + chr5_3:30528178-30528197 Msa0807660:CDS 40.0%
TACTCACATAGCTTCTTGCA+CGG - chr5_3:30528336-30528355 None:intergenic 40.0%
TCTATGGTTGTTGAAGCAAG+TGG + chr5_3:30528598-30528617 Msa0807660:CDS 40.0%
! CATCTTTTTCCCCGAATGTA+AGG - chr5_3:30528633-30528652 None:intergenic 40.0%
ACTACATTGACAAAGTGAGG+CGG + chr5_3:30528659-30528678 Msa0807660:CDS 40.0%
! AGGGCTGCTTATGAGTATTT+TGG + chr5_3:30528823-30528842 Msa0807660:CDS 40.0%
CATGGTCAATCAGTTCTGTT+AGG + chr5_3:30528925-30528944 Msa0807660:CDS 40.0%
AGTATCCTCCTTCGACAATA+GGG - chr5_3:30528956-30528975 None:intergenic 40.0%
TAGTATCCTCCTTCGACAAT+AGG - chr5_3:30528957-30528976 None:intergenic 40.0%
ACTTGGTTAGAATGTATGCG+TGG + chr5_3:30529000-30529019 Msa0807660:CDS 40.0%
TGGTCAGTAATTATGGCTTG+TGG - chr5_3:30529033-30529052 None:intergenic 40.0%
ACAAGCCATAATTACTGACC+AGG + chr5_3:30529032-30529051 Msa0807660:CDS 40.0%
TCCATCCTGGTCAGTAATTA+TGG - chr5_3:30529040-30529059 None:intergenic 40.0%
AGCAATGAAGAATGCAGTTG+AGG + chr5_3:30529059-30529078 Msa0807660:CDS 40.0%
AATGTGTCTCTCACATACAC+AGG - chr5_3:30529321-30529340 None:intergenic 40.0%
! TGCTTTCTTTGAGAGAATGG+AGG + chr5_3:30529617-30529636 Msa0807660:CDS 40.0%
CTGTGAGCTTAAGAACACAA+AGG - chr5_3:30529794-30529813 None:intergenic 40.0%
TTAATATCCTTCCCCCATCT+TGG - chr5_3:30529852-30529871 None:intergenic 40.0%
ATTGTGAAAAGGAAGCTTGC+TGG + chr5_3:30530182-30530201 Msa0807660:CDS 40.0%
GCTGGAAAGAAAGGACAAAA+AGG + chr5_3:30530200-30530219 Msa0807660:CDS 40.0%
TTGGAATCGACGGTGAATGT+TGG - chr5_3:30527818-30527837 None:intergenic 45.0%
TTGGAGGTTAATGACCAAGC+TGG + chr5_3:30528550-30528569 Msa0807660:CDS 45.0%
TGGAGGTTAATGACCAAGCT+GGG + chr5_3:30528551-30528570 Msa0807660:CDS 45.0%
GCCATAATTACTGACCAGGA+TGG + chr5_3:30529036-30529055 Msa0807660:CDS 45.0%
CATTCTTCATTGCTCCATCC+TGG - chr5_3:30529053-30529072 None:intergenic 45.0%
TGTTTGATGAGCGTCATCGT+TGG + chr5_3:30529292-30529311 Msa0807660:CDS 45.0%
GTTTGATGAGCGTCATCGTT+GGG + chr5_3:30529293-30529312 Msa0807660:CDS 45.0%
TATATTGCCAAGATGGGGGA+AGG + chr5_3:30529842-30529861 Msa0807660:CDS 45.0%
ACGCTGCAACTCTTCATTTC+CGG - chr5_3:30529916-30529935 None:intergenic 45.0%
CTGTAGCGACTCGAAGTAAA+GGG + chr5_3:30530120-30530139 Msa0807660:CDS 45.0%
!! GAAGCCTTCCTTTTTGAAGG+AGG - chr5_3:30530149-30530168 None:intergenic 45.0%
!! AGGAAGCTTGCTGGAAAGAA+AGG + chr5_3:30530191-30530210 Msa0807660:CDS 45.0%
TTTGGGCAGATGCGAGATGT+AGG + chr5_3:30528803-30528822 Msa0807660:CDS 50.0%
TTGGGCAGATGCGAGATGTA+GGG + chr5_3:30528804-30528823 Msa0807660:CDS 50.0%
! GTGAGAGACACATTTTGGGC+TGG + chr5_3:30529327-30529346 Msa0807660:CDS 50.0%
CCTTTACTTCGAGTCGCTAC+AGG - chr5_3:30530122-30530141 None:intergenic 50.0%
CCTGTAGCGACTCGAAGTAA+AGG + chr5_3:30530119-30530138 Msa0807660:CDS 50.0%
!! AAGGGCGTCCTCCTTCAAAA+AGG + chr5_3:30530138-30530157 Msa0807660:CDS 50.0%
GCGTCCTCCTTCAAAAAGGA+AGG + chr5_3:30530142-30530161 Msa0807660:CDS 50.0%
AAAGTGAGGCGGCTTCGACT+TGG + chr5_3:30528670-30528689 Msa0807660:CDS 55.0%
AAGTGAGGCGGCTTCGACTT+GGG + chr5_3:30528671-30528690 Msa0807660:CDS 55.0%
AGGTTGCGCCCTATTGTCGA+AGG + chr5_3:30528945-30528964 Msa0807660:CDS 55.0%
TTGCGCCCTATTGTCGAAGG+AGG + chr5_3:30528948-30528967 Msa0807660:CDS 55.0%
! ATGGCGAGCTTTCGGGTAGA+CGG - chr5_3:30529085-30529104 None:intergenic 55.0%
TCTACCCGAAAGCTCGCCAT+CGG + chr5_3:30529085-30529104 Msa0807660:CDS 55.0%
TATGCCACAAGCACCACCGA+TGG - chr5_3:30529104-30529123 None:intergenic 55.0%
ACCACCGATGGCGAGCTTTC+GGG - chr5_3:30529092-30529111 None:intergenic 60.0%
CACCACCGATGGCGAGCTTT+CGG - chr5_3:30529093-30529112 None:intergenic 60.0%
!! AGGCGGCTTCGACTTGGGAC+AGG + chr5_3:30528676-30528695 Msa0807660:CDS 65.0%
ACCCGAAAGCTCGCCATCGG+TGG + chr5_3:30529088-30529107 Msa0807660:CDS 65.0%
CTCGCCATCGGTGGTGCTTG+TGG + chr5_3:30529097-30529116 Msa0807660:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 30527528 30530304 30527528 ID=Msa0807660;Name=Msa0807660
chr5_3 mRNA 30527528 30530304 30527528 ID=Msa0807660-mRNA-1;Parent=Msa0807660;Name=Msa0807660-mRNA-1;_AED=0.23;_eAED=0.36;_QI=0|0|0|0.5|0|1|2|0|742
chr5_3 exon 30527528 30527534 30527528 ID=Msa0807660-mRNA-1:exon:10226;Parent=Msa0807660-mRNA-1
chr5_3 exon 30528083 30530304 30528083 ID=Msa0807660-mRNA-1:exon:10227;Parent=Msa0807660-mRNA-1
chr5_3 CDS 30527528 30527534 30527528 ID=Msa0807660-mRNA-1:cds;Parent=Msa0807660-mRNA-1
chr5_3 CDS 30528083 30530304 30528083 ID=Msa0807660-mRNA-1:cds;Parent=Msa0807660-mRNA-1
Gene Sequence

>Msa0807660

ATGTCTGTGGAGAAAACTTTTAGATCTTTTGAAGAAGAAGAAGAAGAAGAAGAAGAGAGTGATCACATAAACGAAAATTGTGAGGATAATTTAACAGAAAATGAAGATGATGAACCTAAGGTTGGCATGAAATTTAATTCTGCAGAGGAAGTTGACAAATATTACAAGAATTATGCAAGATGTATAGGTTTTGGAGTTAGCATAATCAGTACAAAAAAGGGAGATGATGGAAAAAAGTATTTTACTCTAGGGTGTAACCGTGCAAGAAGCTATGTGAGTAAGTCCAAAAATCTTTTAAAGCCAAATCTTAGCATGAAAACACACTGTAAGGCTAGAATAAATGTATGTGTGTCTTCAGATGGAACAACTACTATTTCTAAGGTTGCTCTTGAACATAATCATGACTTAATAAGTCCAAGCAAATCAAGATATTTTAGATGTAACAAGATTATTGACCCTAGTATAAAAAGGAGATTGGAGGTTAATGACCAAGCTGGGATTAATGTAAGTAGAAATTTTCGGTCTATGGTTGTTGAAGCAAGTGGATATGATAACCTTACATTCGGGGAAAAAGATGTTCGAAACTACATTGACAAAGTGAGGCGGCTTCGACTTGGGACAGGAGACGCTGAAGCCATTCAAAATTATTTTGTTAGAATGCAAAGACAAAATAATGAATTTTATTATGTAATGGATGTGGATGAAAAAAGTCGTTTACGAAATGTTTTTTGGGCAGATGCGAGATGTAGGGCTGCTTATGAGTATTTTGGCGAAGTAATAACCTTTGATACCACTTACTTAACAAATAAATATGACATGCCTTTTGCTCCTTTTGTTGAAGTAAATCATCATGGTCAATCAGTTCTGTTAGGTTGCGCCCTATTGTCGAAGGAGGATACTAAAACTTTTTCTTGGTTGTTCACTACTTGGTTAGAATGTATGCGTGGACGTGCTCCACAAGCCATAATTACTGACCAGGATGGAGCAATGAAGAATGCAGTTGAGGCCGTCTACCCGAAAGCTCGCCATCGGTGGTGCTTGTGGCATATAATGAAAAAGGTTCCAGAAAAATTGAGTAGACTCTCTAACTATGAGTCTATCAAAATACTTTTGCATGATGCGGTGTATGATTCTTTGAGCATAAGTGATTTCATGGAGAAGTGGGAAAAAATTATTGAATGTTATGAACTTCATGATAATGAATGGTTGAAAGGGTTGTTTGATGAGCGTCATCGTTGGGTTCCTGTGTATGTGAGAGACACATTTTGGGCTGGAATGTCAACTACACAACGAAGTGAAAGTATGAACGCATTTTTTGATGGATATGTGCACTCAAAGACATCATTGAAGCAATTTGTTGAACAATATGATAATGCATTGAAAGATAAAATTGAAAAGGAAAATATGGCTGATTTTAGTTCATCCAATAGAATTATTGCTTGTATAAGTCATTTTGGATTTGAATCTCAATTCCAAAAGGCATATACCAATGCAAAATTCAAAGAGTTTCAAGTAGAAGTGGCTTCTATGATGTACTGTCATGCTTTCTTTGAGAGAATGGAGGCTTTGAACTTAACATATTGTGTGACAGAAAGTAAAAAGGTATTTGATACAGTTAAAGACATAATGTTCAAGGTATTCTTCAATGAGAAAGACTTTGAAATACGATGTACATGTTGTTTATTTGAGTTTAAGGGTATTTTATGTAGACATATCCTTTGTGTTCTTAAGCTCACAGCTAAAACAGAGTCAGTGCCCTCTTGTTATATATTGCCAAGATGGGGGAAGGATATTAAGCGAAGATATACACTTATTAAAAGTGGTTTTGATCAATTAGCCGGAAATGAAGAGTTGCAGCGTGTAGCTAAAGCTTGTGATGCCTTTTACGAAGTTGCTTCTTCATGTATCCATACTGAAAATGATTTATCGAAAGTAATGGATAGAATAGAGAACTTAAAACTGGAGATGACATGTCATGAAAGTTTTTCAGAGATCACTGAAGGAGATAACTTAGTTCAAAATCAGATGACTAAAATTCTTGATCCTGTAGCGACTCGAAGTAAAGGGCGTCCTCCTTCAAAAAGGAAGGCTTCTAAAGTTGATCAAATTGTGAAAAGGAAGCTTGCTGGAAAGAAAGGACAAAAAGGCAACAAAAAAAGTAACACTTATCAAAGTCAAGAAGAGGTAATCTTGAAATTTTCAAGTTTATTTTATCTCCCTTACACGTAA
Protein sequence

>Msa0807660

MSVEKTFRSFEEEEEEEEESDHINENCEDNLTENEDDEPKVGMKFNSAEEVDKYYKNYARCIGFGVSIISTKKGDDGKKYFTLGCNRARSYVSKSKNLLKPNLSMKTHCKARINVCVSSDGTTTISKVALEHNHDLISPSKSRYFRCNKIIDPSIKRRLEVNDQAGINVSRNFRSMVVEASGYDNLTFGEKDVRNYIDKVRRLRLGTGDAEAIQNYFVRMQRQNNEFYYVMDVDEKSRLRNVFWADARCRAAYEYFGEVITFDTTYLTNKYDMPFAPFVEVNHHGQSVLLGCALLSKEDTKTFSWLFTTWLECMRGRAPQAIITDQDGAMKNAVEAVYPKARHRWCLWHIMKKVPEKLSRLSNYESIKILLHDAVYDSLSISDFMEKWEKIIECYELHDNEWLKGLFDERHRWVPVYVRDTFWAGMSTTQRSESMNAFFDGYVHSKTSLKQFVEQYDNALKDKIEKENMADFSSSNRIIACISHFGFESQFQKAYTNAKFKEFQVEVASMMYCHAFFERMEALNLTYCVTESKKVFDTVKDIMFKVFFNEKDFEIRCTCCLFEFKGILCRHILCVLKLTAKTESVPSCYILPRWGKDIKRRYTLIKSGFDQLAGNEELQRVAKACDAFYEVASSCIHTENDLSKVMDRIENLKLEMTCHESFSEITEGDNLVQNQMTKILDPVATRSKGRPPSKRKASKVDQIVKRKLAGKKGQKGNKKSNTYQSQEEVILKFSSLFYLPYT*