Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0807720 | XP_039687084.1 | 80.328 | 122 | 24 | 0 | 5 | 126 | 1 | 122 | 1.71e-65 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0807720 | G7INX6 | 80.328 | 122 | 24 | 0 | 5 | 126 | 1 | 122 | 8.17e-66 | 204 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0807720 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0807720 | MtrunA17_Chr2g0281511 | 80.328 | 122 | 24 | 0 | 5 | 126 | 1 | 122 | 1.57e-69 | 204 |
| Msa0807720 | MtrunA17_Chr2g0309121 | 77.869 | 122 | 26 | 1 | 5 | 126 | 1 | 121 | 2.42e-64 | 191 |
| Msa0807720 | MtrunA17_Chr3g0081891 | 78.481 | 79 | 17 | 0 | 5 | 83 | 1 | 79 | 1.21e-39 | 127 |
| Msa0807720 | MtrunA17_Chr7g0276661 | 30.645 | 124 | 69 | 3 | 15 | 123 | 45 | 166 | 5.26e-16 | 70.1 |
| Msa0807720 | MtrunA17_Chr0c01g0489371 | 40.659 | 91 | 52 | 1 | 26 | 114 | 224 | 314 | 8.35e-16 | 71.6 |
| Msa0807720 | MtrunA17_Chr8g0356961 | 39.326 | 89 | 52 | 1 | 28 | 114 | 77 | 165 | 5.25e-15 | 67.4 |
| Msa0807720 | MtrunA17_Chr8g0372431 | 27.835 | 97 | 70 | 0 | 28 | 124 | 6 | 102 | 6.79e-14 | 62.8 |
| Msa0807720 | MtrunA17_Chr8g0356951 | 33.663 | 101 | 63 | 2 | 28 | 124 | 217 | 317 | 8.93e-13 | 63.2 |
| Msa0807720 | MtrunA17_Chr5g0434641 | 32.967 | 91 | 59 | 1 | 26 | 114 | 208 | 298 | 1.39e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.123735 | 5_3:+30931654 | Msa0807720:CDS |
| AAAATTCCAATGTTCATTAT+TGG | 0.164838 | 5_3:+30931626 | Msa0807720:CDS |
| GAAGTCAAAGATGTTGAATT+AGG | 0.190003 | 5_3:+30931595 | Msa0807720:CDS |
| TACTAGTTTCTTCATCAAAA+AGG | 0.264017 | 5_3:-30931848 | None:intergenic |
| TGTGTGCTACCGTCGTTAAC+TGG | 0.296602 | 5_3:+30932015 | Msa0807720:CDS |
| CCTCGACGTGTTGCCGAGAA+TGG | 0.307864 | 5_3:+30931798 | Msa0807720:CDS |
| CGGTCAATAATCCTCCTAAT+AGG | 0.355854 | 5_3:+30931991 | Msa0807720:CDS |
| TTTGGATGGAAACAATGTTT+TGG | 0.356448 | 5_3:+30931672 | Msa0807720:CDS |
| AAGTCAAAGATGTTGAATTA+GGG | 0.382701 | 5_3:+30931596 | Msa0807720:CDS |
| ACGGTAGCACACATCCTATT+AGG | 0.423167 | 5_3:-30932005 | None:intergenic |
| ATTGGTATACAAACCATTCT+CGG | 0.470714 | 5_3:-30931811 | None:intergenic |
| GTGTGCTACCGTCGTTAACT+GGG | 0.507289 | 5_3:+30932016 | Msa0807720:CDS |
| ATACTTCCAATAATGAACAT+TGG | 0.558425 | 5_3:-30931632 | None:intergenic |
| GCATGGATCAGAAATACATA+GGG | 0.559318 | 5_3:+30931907 | Msa0807720:CDS |
| AGCATGGATCAGAAATACAT+AGG | 0.564126 | 5_3:+30931906 | Msa0807720:CDS |
| CTTGAAAAGTGCCTAAAAGT+TGG | 0.570138 | 5_3:+30931951 | Msa0807720:CDS |
| AGAAATACATAGGGAGAGGA+TGG | 0.573439 | 5_3:+30931916 | Msa0807720:CDS |
| GATCAGAAATACATAGGGAG+AGG | 0.587262 | 5_3:+30931912 | Msa0807720:CDS |
| TCACACTGCGTCAAGAAGCA+TGG | 0.587686 | 5_3:+30931890 | Msa0807720:CDS |
| CCATTCTCGGCAACACGTCG+AGG | 0.599354 | 5_3:-30931798 | None:intergenic |
| GTAGCACACATCCTATTAGG+AGG | 0.627873 | 5_3:-30932002 | None:intergenic |
| TAATCAGTCCCAGTTAACGA+CGG | 0.733495 | 5_3:-30932024 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCCAATGTTCATTAT+TGG | + | chr5_3:30931626-30931645 | Msa0807720:CDS | 20.0% |
| ! | AAGGAAAAAATGAATGAGAT+TGG | + | chr5_3:30931568-30931587 | Msa0807720:CDS | 25.0% |
| ! | AAGTCAAAGATGTTGAATTA+GGG | + | chr5_3:30931596-30931615 | Msa0807720:CDS | 25.0% |
| ! | ATACTTCCAATAATGAACAT+TGG | - | chr5_3:30931635-30931654 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | + | chr5_3:30931654-30931673 | Msa0807720:CDS | 25.0% |
| !! | AAGGAGAATTTTAGATTGAT+TGG | - | chr5_3:30931832-30931851 | None:intergenic | 25.0% |
| !! | TACTAGTTTCTTCATCAAAA+AGG | - | chr5_3:30931851-30931870 | None:intergenic | 25.0% |
| GAAGTCAAAGATGTTGAATT+AGG | + | chr5_3:30931595-30931614 | Msa0807720:CDS | 30.0% | |
| ! | TTTGGATGGAAACAATGTTT+TGG | + | chr5_3:30931672-30931691 | Msa0807720:CDS | 30.0% |
| ATTGGTATACAAACCATTCT+CGG | - | chr5_3:30931814-30931833 | None:intergenic | 30.0% | |
| !! | GTTACTGCTTCTATTTTGGA+TGG | + | chr5_3:30931658-30931677 | Msa0807720:CDS | 35.0% |
| !!! | GGAAACAATGTTTTGGTTAG+TGG | + | chr5_3:30931679-30931698 | Msa0807720:intron | 35.0% |
| AGCATGGATCAGAAATACAT+AGG | + | chr5_3:30931906-30931925 | Msa0807720:CDS | 35.0% | |
| GCATGGATCAGAAATACATA+GGG | + | chr5_3:30931907-30931926 | Msa0807720:CDS | 35.0% | |
| !! | CTTGAAAAGTGCCTAAAAGT+TGG | + | chr5_3:30931951-30931970 | Msa0807720:CDS | 35.0% |
| ! | AAACAACGTCACCAACTTTT+AGG | - | chr5_3:30931965-30931984 | None:intergenic | 35.0% |
| GATCAGAAATACATAGGGAG+AGG | + | chr5_3:30931912-30931931 | Msa0807720:CDS | 40.0% | |
| AGAAATACATAGGGAGAGGA+TGG | + | chr5_3:30931916-30931935 | Msa0807720:CDS | 40.0% | |
| !! | TGGTGACGTTGTTTTGTTCA+CGG | + | chr5_3:30931971-30931990 | Msa0807720:CDS | 40.0% |
| CGGTCAATAATCCTCCTAAT+AGG | + | chr5_3:30931991-30932010 | Msa0807720:CDS | 40.0% | |
| GTAGCACACATCCTATTAGG+AGG | - | chr5_3:30932005-30932024 | None:intergenic | 45.0% | |
| ACGGTAGCACACATCCTATT+AGG | - | chr5_3:30932008-30932027 | None:intergenic | 45.0% | |
| TCACACTGCGTCAAGAAGCA+TGG | + | chr5_3:30931890-30931909 | Msa0807720:CDS | 50.0% | |
| TGTGTGCTACCGTCGTTAAC+TGG | + | chr5_3:30932015-30932034 | Msa0807720:CDS | 50.0% | |
| GTGTGCTACCGTCGTTAACT+GGG | + | chr5_3:30932016-30932035 | Msa0807720:CDS | 50.0% | |
| CCATTCTCGGCAACACGTCG+AGG | - | chr5_3:30931801-30931820 | None:intergenic | 60.0% | |
| CCTCGACGTGTTGCCGAGAA+TGG | + | chr5_3:30931798-30931817 | Msa0807720:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 30931565 | 30932043 | 30931565 | ID=Msa0807720;Name=Msa0807720 |
| chr5_3 | mRNA | 30931565 | 30932043 | 30931565 | ID=Msa0807720-mRNA-1;Parent=Msa0807720;Name=Msa0807720-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|126 |
| chr5_3 | exon | 30931565 | 30931693 | 30931565 | ID=Msa0807720-mRNA-1:exon:10265;Parent=Msa0807720-mRNA-1 |
| chr5_3 | exon | 30931792 | 30932043 | 30931792 | ID=Msa0807720-mRNA-1:exon:10266;Parent=Msa0807720-mRNA-1 |
| chr5_3 | CDS | 30931565 | 30931693 | 30931565 | ID=Msa0807720-mRNA-1:cds;Parent=Msa0807720-mRNA-1 |
| chr5_3 | CDS | 30931792 | 30932043 | 30931792 | ID=Msa0807720-mRNA-1:cds;Parent=Msa0807720-mRNA-1 |
| Gene Sequence |
| Protein sequence |
