AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0808130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0808130 MtrunA17_Chr4g0009901 75.665 263 63 1 1 263 1 262 4.98e-141 399
Msa0808130 MtrunA17_Chr4g0009851 63.245 302 108 3 1 300 1 301 1.17e-131 377
Msa0808130 MtrunA17_Chr4g0009981 61.446 249 94 2 53 300 1 248 1.47e-99 293
Msa0808130 MtrunA17_Chr1g0161431 46.774 248 100 4 10 226 11 257 1.28e-67 222
Msa0808130 MtrunA17_Chr4g0011071 67.391 138 44 1 55 192 35 171 1.96e-63 198
Msa0808130 MtrunA17_Chr5g0423031 96.629 89 3 0 1 89 1 89 6.01e-58 184
Msa0808130 MtrunA17_Chr5g0423031 83.158 95 14 1 185 277 80 174 4.08e-41 141
Msa0808130 MtrunA17_Chr2g0294911 41.445 263 126 4 10 248 29 287 2.17e-57 194
Msa0808130 MtrunA17_Chr4g0067981 43.852 244 108 4 10 226 31 272 6.97e-56 192
Msa0808130 MtrunA17_Chr3g0123131 41.224 245 115 5 10 226 13 256 3.16e-54 186
Msa0808130 MtrunA17_Chr4g0009991 74.138 116 29 1 103 218 2 116 9.86e-53 169
Msa0808130 MtrunA17_Chr6g0471041 39.172 314 151 7 10 309 14 301 3.64e-52 177
Msa0808130 MtrunA17_Chr8g0376881 38.934 244 122 4 9 226 24 266 3.92e-50 174
Msa0808130 MtrunA17_Chr1g0149841 39.759 249 119 5 7 226 2 248 4.81e-49 170
Msa0808130 MtrunA17_Chr3g0137101 40.562 249 115 6 10 226 8 255 1.74e-48 171
Msa0808130 MtrunA17_Chr4g0010001 82.022 89 16 0 1 89 1 89 1.78e-47 154
Msa0808130 MtrunA17_Chr7g0275401 36.653 251 127 5 7 227 2 250 6.24e-47 159
Msa0808130 MtrunA17_Chr8g0391911 39.316 234 117 2 10 218 9 242 7.28e-47 158
Msa0808130 MtrunA17_Chr7g0275891 36.015 261 123 7 7 225 2 260 6.37e-45 156
Msa0808130 MtrunA17_Chr2g0304841 36.471 255 124 5 10 227 18 271 9.39e-45 161
Msa0808130 MtrunA17_Chr4g0074411 36.694 248 131 4 10 232 18 264 2.97e-44 159
Msa0808130 MtrunA17_Chr8g0383961 42.128 235 104 8 1 230 1 208 4.64e-43 149
Msa0808130 MtrunA17_Chr4g0074401 36.735 245 126 5 10 226 17 260 6.27e-43 155
Msa0808130 MtrunA17_Chr1g0149851 36.742 264 121 6 7 226 2 263 7.54e-43 154
Msa0808130 MtrunA17_Chr6g0456631 44.444 153 84 1 10 161 9 161 7.42e-40 137
Msa0808130 MtrunA17_Chr3g0134461 35.238 210 110 3 10 218 8 192 1.98e-37 132
Msa0808130 MtrunA17_Chr4g0074421 38.462 182 89 3 59 218 2 182 1.73e-35 127
Msa0808130 MtrunA17_Chr3g0134481 33.962 212 103 3 10 220 8 183 7.88e-34 122
Msa0808130 MtrunA17_Chr1g0149831 36.364 187 86 5 68 226 1 182 8.37e-28 107
Msa0808130 MtrunA17_Chr4g0031961 45.161 93 46 2 135 226 100 188 3.73e-16 77.8
Msa0808130 MtrunA17_Chr7g0261911 52.857 70 32 1 169 237 262 331 5.30e-16 78.2
Msa0808130 MtrunA17_Chr5g0417521 62.963 54 19 1 169 221 15 68 7.79e-16 77.0
Msa0808130 MtrunA17_Chr7g0255711 59.322 59 24 0 168 226 90 148 2.41e-15 75.1
Msa0808130 MtrunA17_Chr6g0461641 58.065 62 23 2 169 229 42 101 2.51e-15 75.9
Msa0808130 MtrunA17_Chr6g0473731 33.540 161 84 5 101 246 21 173 3.17e-15 75.9
Msa0808130 MtrunA17_Chr7g0274471 51.429 70 33 1 169 237 218 287 8.99e-15 74.3
Msa0808130 MtrunA17_Chr2g0283951 37.600 125 67 5 154 268 69 192 9.20e-15 74.3
Msa0808130 MtrunA17_Chr1g0190301 47.126 87 43 2 165 250 171 255 1.19e-14 73.9
Msa0808130 MtrunA17_Chr2g0321461 55.385 65 27 2 158 221 35 98 1.28e-14 73.9
Msa0808130 MtrunA17_Chr3g0095611 50.725 69 33 1 161 228 11 79 3.93e-14 70.1
Msa0808130 MtrunA17_Chr1g0162931 43.182 88 47 2 161 247 10 95 4.98e-14 71.2
Msa0808130 MtrunA17_Chr8g0379121 48.529 68 34 1 155 221 97 164 6.71e-14 72.0
Msa0808130 MtrunA17_Chr7g0243081 55.357 56 24 1 167 221 69 124 1.12e-13 70.9
Msa0808130 MtrunA17_Chr1g0210911 47.143 70 36 1 154 222 7 76 3.75e-13 68.9
Msa0808130 MtrunA17_Chr4g0042451 57.407 54 22 1 169 221 17 70 4.49e-13 68.9
Msa0808130 MtrunA17_Chr7g0254981 44.828 87 45 2 160 243 41 127 5.08e-13 68.2
Msa0808130 MtrunA17_Chr1g0197171 56.897 58 24 1 165 221 40 97 9.27e-13 67.8
Msa0808130 MtrunA17_Chr2g0290991 46.250 80 41 2 143 221 14 92 1.06e-12 67.8
Msa0808130 MtrunA17_Chr2g0324891 31.405 121 82 1 149 269 141 260 1.13e-12 68.2
Msa0808130 MtrunA17_Chr8g0356501 56.897 58 24 1 165 221 33 90 1.90e-12 67.0
Msa0808130 MtrunA17_Chr2g0310011 56.897 58 24 1 165 221 31 88 2.67e-12 66.6
Msa0808130 MtrunA17_Chr7g0258551 36.220 127 66 5 167 289 210 325 3.38e-12 66.6
Msa0808130 MtrunA17_Chr4g0040481 51.724 58 28 0 168 225 157 214 4.34e-12 66.2
Msa0808130 MtrunA17_Chr2g0320231 47.619 63 32 1 161 222 89 151 4.35e-12 66.2
Msa0808130 MtrunA17_Chr2g0288601 53.704 54 24 1 166 218 68 121 4.42e-12 62.4
Msa0808130 MtrunA17_Chr1g0175071 55.556 63 27 1 163 224 171 233 4.86e-12 65.9
Msa0808130 MtrunA17_Chr4g0054981 49.315 73 36 1 164 236 305 376 5.42e-12 66.2
Msa0808130 MtrunA17_Chr2g0291021 54.795 73 29 3 169 237 49 121 7.91e-12 65.1
Msa0808130 MtrunA17_Chr5g0400241 46.479 71 38 0 158 228 300 370 8.36e-12 65.9
Msa0808130 MtrunA17_Chr5g0400241 29.921 127 83 4 10 134 14 136 3.72e-11 63.9
Msa0808130 MtrunA17_Chr1g0175091 43.243 74 39 2 167 239 256 327 1.46e-11 64.7
Msa0808130 MtrunA17_Chr5g0409731 55.556 54 23 1 169 221 25 78 1.49e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0808130 AT2G01760.1 40.784 255 115 5 7 226 4 257 1.37e-53 180
Msa0808130 AT3G16857.1 39.924 263 111 3 10 226 33 294 1.97e-53 185
Msa0808130 AT3G16857.2 39.924 263 111 3 10 226 33 294 2.28e-53 186
Msa0808130 AT1G67710.1 40.816 245 115 5 11 226 8 251 8.84e-49 170
Msa0808130 AT2G01760.2 36.749 283 115 6 7 226 4 285 1.26e-47 165
Msa0808130 AT2G25180.1 39.004 241 122 5 10 226 13 252 9.59e-45 160
Msa0808130 AT4G31920.1 38.889 234 128 5 10 230 13 244 2.01e-42 154
Msa0808130 AT3G16857.3 38.839 224 90 3 49 226 2 224 1.14e-40 150
Msa0808130 AT5G58080.1 37.209 258 133 6 10 244 14 265 2.75e-40 149
Msa0808130 AT2G01760.3 38.342 193 84 4 68 226 1 192 5.27e-33 124
Msa0808130 AT3G62670.1 33.929 224 124 6 27 227 52 274 1.20e-32 125
Msa0808130 AT1G49190.1 31.429 245 132 6 10 221 30 271 5.51e-31 120
Msa0808130 AT1G67710.2 39.572 187 84 4 68 226 1 186 1.70e-29 117
Msa0808130 AT5G49240.1 31.646 237 134 6 9 218 37 272 3.65e-29 113
Msa0808130 AT5G07210.2 27.306 271 140 7 14 230 16 283 2.10e-21 95.1
Msa0808130 AT1G67710.3 38.922 167 73 4 88 226 12 177 4.83e-21 93.2
Msa0808130 AT5G07210.1 26.882 279 139 9 14 230 16 291 3.46e-19 88.6
Msa0808130 AT1G49190.2 36.508 126 78 2 10 134 30 154 1.04e-17 84.0
Msa0808130 AT1G68210.1 26.296 270 141 8 8 221 7 274 3.89e-17 82.4
Msa0808130 AT5G59570.2 56.667 60 26 0 167 226 139 198 1.22e-15 76.3
Msa0808130 AT5G59570.1 56.667 60 26 0 167 226 139 198 1.22e-15 76.3
Msa0808130 AT5G05090.1 59.322 59 24 0 168 226 80 138 1.38e-15 75.9
Msa0808130 AT2G40970.1 57.627 59 25 0 168 226 104 162 2.31e-15 74.7
Msa0808130 AT3G10760.1 59.322 59 24 0 168 226 104 162 2.54e-15 75.9
Msa0808130 AT3G46640.3 50.685 73 33 1 157 226 129 201 2.60e-15 75.9
Msa0808130 AT3G46640.2 50.685 73 33 1 157 226 129 201 2.98e-15 75.5
Msa0808130 AT3G46640.1 50.685 73 33 1 157 226 129 201 2.98e-15 75.5
Msa0808130 AT3G04030.1 56.923 65 26 2 158 221 35 98 4.40e-15 75.5
Msa0808130 AT3G04030.3 56.923 65 26 2 158 221 35 98 4.57e-15 75.5
Msa0808130 AT5G18240.2 56.923 65 26 2 158 221 35 98 1.11e-14 74.3
Msa0808130 AT5G18240.3 56.923 65 26 2 158 221 35 98 1.11e-14 74.3
Msa0808130 AT5G18240.1 56.923 65 26 2 158 221 35 98 1.21e-14 74.3
Msa0808130 AT5G18240.4 56.923 65 26 2 158 221 35 98 1.21e-14 74.3
Msa0808130 AT5G18240.5 56.923 65 26 2 158 221 35 98 1.26e-14 74.3
Msa0808130 AT4G04605.1 45.455 77 40 2 167 241 12 88 2.29e-14 71.6
Msa0808130 AT3G04030.2 53.125 64 29 1 158 221 35 97 4.44e-14 72.8
Msa0808130 AT2G01060.1 50.588 85 38 3 158 238 4 88 4.65e-14 71.6
Msa0808130 AT4G13640.1 52.778 72 30 2 165 232 33 104 4.66e-14 71.6
Msa0808130 AT4G13640.2 52.778 72 30 2 165 232 33 104 4.89e-14 71.6
Msa0808130 AT5G44190.1 56.897 58 25 0 168 225 146 203 8.37e-14 71.6
Msa0808130 AT2G20570.1 42.529 87 44 1 145 225 123 209 9.94e-14 71.6
Msa0808130 AT5G44190.2 56.897 58 25 0 168 225 146 203 1.01e-13 71.2
Msa0808130 AT2G40260.1 47.945 73 37 1 151 222 64 136 2.28e-13 70.5
Msa0808130 AT5G06800.1 46.835 79 35 3 169 240 192 270 2.91e-13 70.1
Msa0808130 AT5G29000.4 36.697 109 61 2 129 236 198 299 2.94e-13 69.7
Msa0808130 AT3G24120.1 56.897 58 24 1 165 221 37 94 3.05e-13 69.3
Msa0808130 AT3G24120.2 56.897 58 24 1 165 221 37 94 3.17e-13 69.3
Msa0808130 AT5G29000.1 35.714 112 64 2 129 239 155 259 5.57e-13 69.3
Msa0808130 AT5G29000.3 35.714 112 64 2 129 239 155 259 5.57e-13 69.3
Msa0808130 AT1G14600.1 59.259 54 21 1 170 222 24 77 5.68e-13 68.2
Msa0808130 AT5G29000.2 35.714 112 64 2 129 239 198 302 5.90e-13 69.3
Msa0808130 AT2G42660.1 47.692 65 33 1 159 222 40 104 6.62e-13 67.8
Msa0808130 AT5G06800.2 45.238 84 34 3 169 240 192 275 7.52e-13 68.9
Msa0808130 AT2G20570.2 58.182 55 23 0 171 225 171 225 9.81e-13 68.9
Msa0808130 AT4G28610.1 47.297 74 37 2 169 241 225 297 1.90e-12 67.8
Msa0808130 AT5G06800.3 57.407 54 22 1 169 221 192 245 2.76e-12 66.6
Msa0808130 AT2G02060.2 50.000 62 30 1 170 230 30 91 3.11e-12 65.9
Msa0808130 AT2G02060.1 50.000 62 30 1 170 230 30 91 3.11e-12 65.9
Msa0808130 AT2G38300.1 50.877 57 27 1 167 222 52 108 3.47e-12 66.6
Msa0808130 AT3G12730.1 53.448 58 26 1 165 221 19 76 4.06e-12 65.5
Msa0808130 AT2G20400.2 59.259 54 21 1 169 221 231 284 4.13e-12 66.6
Msa0808130 AT2G20400.1 59.259 54 21 1 169 221 231 284 4.20e-12 66.6
Msa0808130 AT1G79430.2 56.897 58 24 1 165 221 30 87 5.28e-12 66.2
Msa0808130 AT3G04450.1 50.000 66 23 2 166 221 226 291 6.75e-12 66.2
Msa0808130 AT1G69580.1 36.066 122 67 5 169 284 34 150 7.08e-12 65.9
Msa0808130 AT3G04450.4 50.000 66 23 2 166 221 186 251 7.74e-12 65.9
Msa0808130 AT3G04450.2 50.000 66 23 2 166 221 186 251 7.74e-12 65.9
Msa0808130 AT1G69580.2 55.556 54 23 1 169 221 34 87 7.82e-12 65.5
Msa0808130 AT3G04450.3 50.000 66 23 2 166 221 226 291 8.13e-12 65.5
Msa0808130 AT5G24470.1 29.032 124 83 3 13 132 49 171 8.37e-12 66.2
Msa0808130 AT5G45580.2 50.769 65 31 1 158 221 12 76 9.36e-12 65.1
Msa0808130 AT5G45580.1 55.172 58 25 1 165 221 3 60 9.65e-12 64.7
Msa0808130 AT3G13040.3 48.529 68 32 2 168 232 240 307 1.31e-11 65.5
Msa0808130 AT3G13040.1 48.529 68 32 2 168 232 240 307 1.31e-11 65.5
Msa0808130 AT3G13040.2 48.529 68 32 2 168 232 240 307 1.31e-11 65.5
Msa0808130 AT4G18020.3 46.970 66 34 1 160 225 288 352 5.68e-11 63.5
Msa0808130 AT4G18020.2 46.970 66 34 1 160 225 288 352 5.68e-11 63.5
Msa0808130 AT4G18020.1 46.970 66 34 1 160 225 288 352 5.68e-11 63.5
Msa0808130 AT4G18020.6 46.970 66 34 1 160 225 288 352 5.68e-11 63.5
Msa0808130 AT4G18020.9 46.970 66 34 1 160 225 288 352 5.68e-11 63.5
Msa0808130 AT4G18020.8 46.970 66 34 1 160 225 288 352 5.76e-11 63.5
Msa0808130 AT4G18020.11 46.970 66 34 1 160 225 288 352 5.76e-11 63.5
Msa0808130 AT4G18020.4 46.970 66 34 1 160 225 288 352 5.76e-11 63.5
Msa0808130 AT4G18020.5 46.970 66 34 1 160 225 288 352 5.76e-11 63.5
Msa0808130 AT4G18020.10 46.970 66 34 1 160 225 288 352 5.76e-11 63.5
Msa0808130 AT4G18020.7 46.970 66 34 1 160 225 288 352 5.76e-11 63.5

Find 57 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAAAATTGGATGGAAAT+TGG 0.274934 5_3:-31888655 Msa0808130:CDS
CATATGGGGCTTGCGAATAT+TGG 0.282422 5_3:-31889364 Msa0808130:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 5_3:-31888959 Msa0808130:intron
AATTCATCAGTTGATTATTT+CGG 0.292540 5_3:-31888565 Msa0808130:CDS
AAGATGATGCACAACAAAAT+TGG 0.295199 5_3:-31888666 Msa0808130:CDS
GAATTAATCACGACACAATC+TGG 0.322128 5_3:+31888583 None:intergenic
TTCTGATGCCTTGAACTTGT+TGG 0.365455 5_3:-31889646 Msa0808130:CDS
GTTACAAAGTCTATCACATA+TGG 0.379871 5_3:-31889380 Msa0808130:CDS
GAACATCATCATGTTGGTTC+CGG 0.380466 5_3:+31888731 None:intergenic
CTTGAACTTGTTGGAAAGAA+AGG 0.398808 5_3:-31889637 Msa0808130:CDS
CAAGTAACTAACCTTGATAA+TGG 0.405402 5_3:+31889777 None:intergenic
CTTTCTTTCCAACAAGTTCA+AGG 0.409488 5_3:+31889638 None:intergenic
GCAATAACGTTGAGACCATC+AGG 0.409612 5_3:+31889854 None:intergenic
TTCCGGTGACCATGACAAAC+GGG 0.410735 5_3:+31889172 None:intergenic
CCCGCGGATAACTTTCATCT+TGG 0.418067 5_3:+31888692 None:intergenic
ACATCTCCTCAATGGTGTTA+AGG 0.419121 5_3:+31889810 None:intergenic
ATTGGAAGCTTTGATGTTCA+AGG 0.429684 5_3:-31889254 Msa0808130:CDS
ATGTTCAAGGTCATAGAAAA+CGG 0.433159 5_3:-31889241 Msa0808130:CDS
ATCAGAAGCTGTAATGCATT+TGG 0.433763 5_3:+31889662 None:intergenic
AAGTCATAGGCATCCATGTT+TGG 0.461471 5_3:+31889579 None:intergenic
TGGCAGCATGTTGTAAGAAA+AGG 0.472283 5_3:-31889305 Msa0808130:CDS
TTACAAAGTCTATCACATAT+GGG 0.475108 5_3:-31889379 Msa0808130:CDS
CATCTTGAACATCATCATGT+TGG 0.479924 5_3:+31888725 None:intergenic
TTGAGACCATCAGGGAATGA+TGG 0.480727 5_3:+31889863 None:intergenic
ATGATGATGTTCAAGATGAA+TGG 0.487260 5_3:-31888720 Msa0808130:CDS
TTCAAGCTCATCAGATTGAC+CGG 0.488681 5_3:-31888750 Msa0808130:CDS
GTTCCGGTGACCATGACAAA+CGG 0.489781 5_3:+31889171 None:intergenic
TTGGACCAAGCCGTTGTATG+AGG 0.493917 5_3:-31889345 Msa0808130:CDS
CTCAATGGTGTTAAGGAAAG+TGG 0.494366 5_3:+31889817 None:intergenic
TGTTCAAGGTCATAGAAAAC+GGG 0.495163 5_3:-31889240 Msa0808130:CDS
GCCCCGTTTGTCATGGTCAC+CGG 0.505882 5_3:-31889174 Msa0808130:CDS
ACACAATCTGGGAACAAGTT+GGG 0.508030 5_3:+31888595 None:intergenic
TTATGGGCTGTCAATCAACT+AGG 0.526348 5_3:-31889134 Msa0808130:CDS
AGCATGTTGAACAAAGTCAT+AGG 0.527015 5_3:+31889566 None:intergenic
TTGAATTGTTCCTCATACAA+CGG 0.530062 5_3:+31889335 None:intergenic
CACTTTCCTTAACACCATTG+AGG 0.536078 5_3:-31889816 Msa0808130:CDS
AGGAAGGAACCATACTATCA+AGG 0.538573 5_3:+31889111 None:intergenic
TGTAATCGATGCCATTATCA+AGG 0.541272 5_3:-31889788 Msa0808130:intron
GACACAATCTGGGAACAAGT+TGG 0.557153 5_3:+31888594 None:intergenic
TGATGCACAACAAAATTGGA+TGG 0.559172 5_3:-31888662 Msa0808130:CDS
TCGATTACACATCTCCTCAA+TGG 0.564409 5_3:+31889802 None:intergenic
AATTAATCACGACACAATCT+GGG 0.566059 5_3:+31888584 None:intergenic
CGAAGAAGCCCCGTTTGTCA+TGG 0.567178 5_3:-31889181 Msa0808130:CDS
TTTGTAACAGAACTTAATGT+GGG 0.567790 5_3:+31889395 None:intergenic
TGAGACCATCAGGGAATGAT+GGG 0.571071 5_3:+31889864 None:intergenic
GATGTTCATGGTTTGAGTGT+AGG 0.572619 5_3:-31888982 Msa0808130:CDS
CTTTGTAACAGAACTTAATG+TGG 0.582713 5_3:+31889394 None:intergenic
TTGTTCCTCATACAACGGCT+TGG 0.596667 5_3:+31889340 None:intergenic
TGATGCTCATATGCCAAACA+TGG 0.604750 5_3:-31889592 Msa0808130:CDS
CCAAGATGAAAGTTATCCGC+GGG 0.608633 5_3:-31888692 Msa0808130:CDS
AGGAACAATTCAAGATGATG+TGG 0.609752 5_3:-31889325 Msa0808130:CDS
TGTGCATCATCTTGTACCCG+CGG 0.630231 5_3:+31888676 None:intergenic
GACAACCCATCATTCCCTGA+TGG 0.634739 5_3:-31889869 Msa0808130:CDS
TACAAAGTCTATCACATATG+GGG 0.656295 5_3:-31889378 Msa0808130:CDS
ACCAAGATGAAAGTTATCCG+CGG 0.659940 5_3:-31888693 Msa0808130:CDS
CAATAACGTTGAGACCATCA+GGG 0.683752 5_3:+31889855 None:intergenic
TCCGGTGACCATGACAAACG+GGG 0.701794 5_3:+31889173 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAACATGAAAATAT+TGG - chr5_3:31889173-31889192 Msa0808130:CDS 10.0%
!! AATAAAAAACATGAAAATAT+TGG - chr5_3:31889173-31889192 Msa0808130:CDS 10.0%
!! TTTATGGAGTTTATTATAAT+TGG - chr5_3:31889580-31889599 Msa0808130:CDS 15.0%
!! TTTATGGAGTTTATTATAAT+TGG - chr5_3:31889580-31889599 Msa0808130:CDS 15.0%
!! AAGAAAAACTTACTAATAAC+AGG + chr5_3:31888923-31888942 None:intergenic 20.0%
!! TGTTTGTATTATTTAACAAC+TGG - chr5_3:31889374-31889393 Msa0808130:CDS 20.0%
!!! ATCTTTTTGAGAATCATTTT+AGG + chr5_3:31889442-31889461 None:intergenic 20.0%
!! TAAAATGATTCTCAAAAAGA+TGG - chr5_3:31889441-31889460 Msa0808130:intron 20.0%
!! AATTCATCAGTTGATTATTT+CGG - chr5_3:31889880-31889899 Msa0808130:CDS 20.0%
!! AAGAAAAACTTACTAATAAC+AGG + chr5_3:31888923-31888942 None:intergenic 20.0%
!! TGTTTGTATTATTTAACAAC+TGG - chr5_3:31889374-31889393 Msa0808130:CDS 20.0%
!!! ATCTTTTTGAGAATCATTTT+AGG + chr5_3:31889442-31889461 None:intergenic 20.0%
!! TAAAATGATTCTCAAAAAGA+TGG - chr5_3:31889441-31889460 Msa0808130:intron 20.0%
!! AATTCATCAGTTGATTATTT+CGG - chr5_3:31889880-31889899 Msa0808130:CDS 20.0%
!!! AACTCTAGAATTGATGAATA+AGG + chr5_3:31888704-31888723 None:intergenic 25.0%
! TTTGTAACAGAACTTAATGT+GGG + chr5_3:31889053-31889072 None:intergenic 25.0%
! TTACAAAGTCTATCACATAT+GGG - chr5_3:31889066-31889085 Msa0808130:intron 25.0%
! TTAAAATCACACAAAACATG+TGG + chr5_3:31889553-31889572 None:intergenic 25.0%
! AAAAAAGTTGCGAATATTGT+CGG + chr5_3:31889821-31889840 None:intergenic 25.0%
!!! AACTCTAGAATTGATGAATA+AGG + chr5_3:31888704-31888723 None:intergenic 25.0%
! TTTGTAACAGAACTTAATGT+GGG + chr5_3:31889053-31889072 None:intergenic 25.0%
! TTACAAAGTCTATCACATAT+GGG - chr5_3:31889066-31889085 Msa0808130:intron 25.0%
! TTAAAATCACACAAAACATG+TGG + chr5_3:31889553-31889572 None:intergenic 25.0%
! AAAAAAGTTGCGAATATTGT+CGG + chr5_3:31889821-31889840 None:intergenic 25.0%
CAAGTAACTAACCTTGATAA+TGG + chr5_3:31888671-31888690 None:intergenic 30.0%
! CATTATCAAGGTTAGTTACT+TGG - chr5_3:31888669-31888688 Msa0808130:CDS 30.0%
CTTTGTAACAGAACTTAATG+TGG + chr5_3:31889054-31889073 None:intergenic 30.0%
GTTACAAAGTCTATCACATA+TGG - chr5_3:31889065-31889084 Msa0808130:intron 30.0%
TACAAAGTCTATCACATATG+GGG - chr5_3:31889067-31889086 Msa0808130:intron 30.0%
TTGAATTGTTCCTCATACAA+CGG + chr5_3:31889113-31889132 None:intergenic 30.0%
ATGTTCAAGGTCATAGAAAA+CGG - chr5_3:31889204-31889223 Msa0808130:CDS 30.0%
! AACTGCATCAACGATTTTTA+TGG - chr5_3:31889294-31889313 Msa0808130:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG - chr5_3:31889295-31889314 Msa0808130:CDS 30.0%
ACAAAGCGAAGAAAAGAATT+AGG + chr5_3:31889357-31889376 None:intergenic 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG - chr5_3:31889411-31889430 Msa0808130:CDS 30.0%
TCGATTGATGAGAAAAAATG+TGG - chr5_3:31889643-31889662 Msa0808130:CDS 30.0%
CGATTGATGAGAAAAAATGT+GGG - chr5_3:31889644-31889663 Msa0808130:CDS 30.0%
! ATGATGATGTTCAAGATGAA+TGG - chr5_3:31889725-31889744 Msa0808130:intron 30.0%
AAGATGATGCACAACAAAAT+TGG - chr5_3:31889779-31889798 Msa0808130:intron 30.0%
CAACAAAATTGGATGGAAAT+TGG - chr5_3:31889790-31889809 Msa0808130:CDS 30.0%
AATTAATCACGACACAATCT+GGG + chr5_3:31889864-31889883 None:intergenic 30.0%
CAAGTAACTAACCTTGATAA+TGG + chr5_3:31888671-31888690 None:intergenic 30.0%
! CATTATCAAGGTTAGTTACT+TGG - chr5_3:31888669-31888688 Msa0808130:CDS 30.0%
CTTTGTAACAGAACTTAATG+TGG + chr5_3:31889054-31889073 None:intergenic 30.0%
GTTACAAAGTCTATCACATA+TGG - chr5_3:31889065-31889084 Msa0808130:intron 30.0%
TACAAAGTCTATCACATATG+GGG - chr5_3:31889067-31889086 Msa0808130:intron 30.0%
TTGAATTGTTCCTCATACAA+CGG + chr5_3:31889113-31889132 None:intergenic 30.0%
ATGTTCAAGGTCATAGAAAA+CGG - chr5_3:31889204-31889223 Msa0808130:CDS 30.0%
! AACTGCATCAACGATTTTTA+TGG - chr5_3:31889294-31889313 Msa0808130:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG - chr5_3:31889295-31889314 Msa0808130:CDS 30.0%
ACAAAGCGAAGAAAAGAATT+AGG + chr5_3:31889357-31889376 None:intergenic 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG - chr5_3:31889411-31889430 Msa0808130:CDS 30.0%
TCGATTGATGAGAAAAAATG+TGG - chr5_3:31889643-31889662 Msa0808130:CDS 30.0%
CGATTGATGAGAAAAAATGT+GGG - chr5_3:31889644-31889663 Msa0808130:CDS 30.0%
! ATGATGATGTTCAAGATGAA+TGG - chr5_3:31889725-31889744 Msa0808130:intron 30.0%
AAGATGATGCACAACAAAAT+TGG - chr5_3:31889779-31889798 Msa0808130:intron 30.0%
CAACAAAATTGGATGGAAAT+TGG - chr5_3:31889790-31889809 Msa0808130:CDS 30.0%
AATTAATCACGACACAATCT+GGG + chr5_3:31889864-31889883 None:intergenic 30.0%
TGTAATCGATGCCATTATCA+AGG - chr5_3:31888657-31888676 Msa0808130:CDS 35.0%
ATCAGAAGCTGTAATGCATT+TGG + chr5_3:31888786-31888805 None:intergenic 35.0%
CTTTCTTTCCAACAAGTTCA+AGG + chr5_3:31888810-31888829 None:intergenic 35.0%
!! CTTGAACTTGTTGGAAAGAA+AGG - chr5_3:31888808-31888827 Msa0808130:CDS 35.0%
AGCATGTTGAACAAAGTCAT+AGG + chr5_3:31888882-31888901 None:intergenic 35.0%
ATTCAGTAGACATCACTACA+AGG + chr5_3:31889028-31889047 None:intergenic 35.0%
AGGAACAATTCAAGATGATG+TGG - chr5_3:31889120-31889139 Msa0808130:intron 35.0%
! ATTGGAAGCTTTGATGTTCA+AGG - chr5_3:31889191-31889210 Msa0808130:CDS 35.0%
TGTTCAAGGTCATAGAAAAC+GGG - chr5_3:31889205-31889224 Msa0808130:CDS 35.0%
AAAAATCGTTGATGCAGTTC+CGG + chr5_3:31889293-31889312 None:intergenic 35.0%
AGCGAAGAAAAGAATTAGGA+AGG + chr5_3:31889353-31889372 None:intergenic 35.0%
CTCAAAAAGATGGATGTTCA+TGG - chr5_3:31889451-31889470 Msa0808130:intron 35.0%
ACATGTTGCTAGTCATTTAC+AGG - chr5_3:31889486-31889505 Msa0808130:intron 35.0%
CATCTTGAACATCATCATGT+TGG + chr5_3:31889723-31889742 None:intergenic 35.0%
TGATGCACAACAAAATTGGA+TGG - chr5_3:31889783-31889802 Msa0808130:intron 35.0%
GAATTAATCACGACACAATC+TGG + chr5_3:31889865-31889884 None:intergenic 35.0%
TGTAATCGATGCCATTATCA+AGG - chr5_3:31888657-31888676 Msa0808130:CDS 35.0%
ATCAGAAGCTGTAATGCATT+TGG + chr5_3:31888786-31888805 None:intergenic 35.0%
CTTTCTTTCCAACAAGTTCA+AGG + chr5_3:31888810-31888829 None:intergenic 35.0%
!! CTTGAACTTGTTGGAAAGAA+AGG - chr5_3:31888808-31888827 Msa0808130:CDS 35.0%
AGCATGTTGAACAAAGTCAT+AGG + chr5_3:31888882-31888901 None:intergenic 35.0%
ATTCAGTAGACATCACTACA+AGG + chr5_3:31889028-31889047 None:intergenic 35.0%
AGGAACAATTCAAGATGATG+TGG - chr5_3:31889120-31889139 Msa0808130:intron 35.0%
! ATTGGAAGCTTTGATGTTCA+AGG - chr5_3:31889191-31889210 Msa0808130:CDS 35.0%
TGTTCAAGGTCATAGAAAAC+GGG - chr5_3:31889205-31889224 Msa0808130:CDS 35.0%
AAAAATCGTTGATGCAGTTC+CGG + chr5_3:31889293-31889312 None:intergenic 35.0%
AGCGAAGAAAAGAATTAGGA+AGG + chr5_3:31889353-31889372 None:intergenic 35.0%
CTCAAAAAGATGGATGTTCA+TGG - chr5_3:31889451-31889470 Msa0808130:intron 35.0%
ACATGTTGCTAGTCATTTAC+AGG - chr5_3:31889486-31889505 Msa0808130:intron 35.0%
CATCTTGAACATCATCATGT+TGG + chr5_3:31889723-31889742 None:intergenic 35.0%
TGATGCACAACAAAATTGGA+TGG - chr5_3:31889783-31889802 Msa0808130:intron 35.0%
GAATTAATCACGACACAATC+TGG + chr5_3:31889865-31889884 None:intergenic 35.0%
CAATAACGTTGAGACCATCA+GGG + chr5_3:31888593-31888612 None:intergenic 40.0%
! CTCAATGGTGTTAAGGAAAG+TGG + chr5_3:31888631-31888650 None:intergenic 40.0%
CACTTTCCTTAACACCATTG+AGG - chr5_3:31888629-31888648 Msa0808130:CDS 40.0%
! ACATCTCCTCAATGGTGTTA+AGG + chr5_3:31888638-31888657 None:intergenic 40.0%
TCGATTACACATCTCCTCAA+TGG + chr5_3:31888646-31888665 None:intergenic 40.0%
TTCTGATGCCTTGAACTTGT+TGG - chr5_3:31888799-31888818 Msa0808130:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG - chr5_3:31888853-31888872 Msa0808130:intron 40.0%
AAGTCATAGGCATCCATGTT+TGG + chr5_3:31888869-31888888 None:intergenic 40.0%
TGGCAGCATGTTGTAAGAAA+AGG - chr5_3:31889140-31889159 Msa0808130:CDS 40.0%
! TTATGGGCTGTCAATCAACT+AGG - chr5_3:31889311-31889330 Msa0808130:CDS 40.0%
! TCAACTAGGCCTTGATAGTA+TGG - chr5_3:31889325-31889344 Msa0808130:CDS 40.0%
AGGAAGGAACCATACTATCA+AGG + chr5_3:31889337-31889356 None:intergenic 40.0%
GATGTTCATGGTTTGAGTGT+AGG - chr5_3:31889463-31889482 Msa0808130:intron 40.0%
TTCAAGCTCATCAGATTGAC+CGG - chr5_3:31889695-31889714 Msa0808130:intron 40.0%
!! GAACATCATCATGTTGGTTC+CGG + chr5_3:31889717-31889736 None:intergenic 40.0%
ACCAAGATGAAAGTTATCCG+CGG - chr5_3:31889752-31889771 Msa0808130:intron 40.0%
ACACAATCTGGGAACAAGTT+GGG + chr5_3:31889853-31889872 None:intergenic 40.0%
CAATAACGTTGAGACCATCA+GGG + chr5_3:31888593-31888612 None:intergenic 40.0%
! CTCAATGGTGTTAAGGAAAG+TGG + chr5_3:31888631-31888650 None:intergenic 40.0%
CACTTTCCTTAACACCATTG+AGG - chr5_3:31888629-31888648 Msa0808130:CDS 40.0%
! ACATCTCCTCAATGGTGTTA+AGG + chr5_3:31888638-31888657 None:intergenic 40.0%
TCGATTACACATCTCCTCAA+TGG + chr5_3:31888646-31888665 None:intergenic 40.0%
TTCTGATGCCTTGAACTTGT+TGG - chr5_3:31888799-31888818 Msa0808130:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG - chr5_3:31888853-31888872 Msa0808130:intron 40.0%
AAGTCATAGGCATCCATGTT+TGG + chr5_3:31888869-31888888 None:intergenic 40.0%
TGGCAGCATGTTGTAAGAAA+AGG - chr5_3:31889140-31889159 Msa0808130:CDS 40.0%
! TTATGGGCTGTCAATCAACT+AGG - chr5_3:31889311-31889330 Msa0808130:CDS 40.0%
! TCAACTAGGCCTTGATAGTA+TGG - chr5_3:31889325-31889344 Msa0808130:CDS 40.0%
AGGAAGGAACCATACTATCA+AGG + chr5_3:31889337-31889356 None:intergenic 40.0%
GATGTTCATGGTTTGAGTGT+AGG - chr5_3:31889463-31889482 Msa0808130:intron 40.0%
TTCAAGCTCATCAGATTGAC+CGG - chr5_3:31889695-31889714 Msa0808130:intron 40.0%
!! GAACATCATCATGTTGGTTC+CGG + chr5_3:31889717-31889736 None:intergenic 40.0%
ACCAAGATGAAAGTTATCCG+CGG - chr5_3:31889752-31889771 Msa0808130:intron 40.0%
ACACAATCTGGGAACAAGTT+GGG + chr5_3:31889853-31889872 None:intergenic 40.0%
TGAGACCATCAGGGAATGAT+GGG + chr5_3:31888584-31888603 None:intergenic 45.0%
TTGAGACCATCAGGGAATGA+TGG + chr5_3:31888585-31888604 None:intergenic 45.0%
GCAATAACGTTGAGACCATC+AGG + chr5_3:31888594-31888613 None:intergenic 45.0%
CATATGGGGCTTGCGAATAT+TGG - chr5_3:31889081-31889100 Msa0808130:intron 45.0%
TTGTTCCTCATACAACGGCT+TGG + chr5_3:31889108-31889127 None:intergenic 45.0%
CCAAGATGAAAGTTATCCGC+GGG - chr5_3:31889753-31889772 Msa0808130:intron 45.0%
GACACAATCTGGGAACAAGT+TGG + chr5_3:31889854-31889873 None:intergenic 45.0%
TGAGACCATCAGGGAATGAT+GGG + chr5_3:31888584-31888603 None:intergenic 45.0%
TTGAGACCATCAGGGAATGA+TGG + chr5_3:31888585-31888604 None:intergenic 45.0%
GCAATAACGTTGAGACCATC+AGG + chr5_3:31888594-31888613 None:intergenic 45.0%
CATATGGGGCTTGCGAATAT+TGG - chr5_3:31889081-31889100 Msa0808130:intron 45.0%
TTGTTCCTCATACAACGGCT+TGG + chr5_3:31889108-31889127 None:intergenic 45.0%
CCAAGATGAAAGTTATCCGC+GGG - chr5_3:31889753-31889772 Msa0808130:intron 45.0%
GACACAATCTGGGAACAAGT+TGG + chr5_3:31889854-31889873 None:intergenic 45.0%
!!! TGATTTTAAATTTAAATTTA+TGG - chr5_3:31889564-31889583 Msa0808130:CDS 5.0%
!!! TGATTTTAAATTTAAATTTA+TGG - chr5_3:31889564-31889583 Msa0808130:CDS 5.0%
GACAACCCATCATTCCCTGA+TGG - chr5_3:31888576-31888595 Msa0808130:CDS 50.0%
TTGGACCAAGCCGTTGTATG+AGG - chr5_3:31889100-31889119 Msa0808130:intron 50.0%
TTCCGGTGACCATGACAAAC+GGG + chr5_3:31889276-31889295 None:intergenic 50.0%
GTTCCGGTGACCATGACAAA+CGG + chr5_3:31889277-31889296 None:intergenic 50.0%
CCCGCGGATAACTTTCATCT+TGG + chr5_3:31889756-31889775 None:intergenic 50.0%
TGTGCATCATCTTGTACCCG+CGG + chr5_3:31889772-31889791 None:intergenic 50.0%
GACAACCCATCATTCCCTGA+TGG - chr5_3:31888576-31888595 Msa0808130:CDS 50.0%
TTGGACCAAGCCGTTGTATG+AGG - chr5_3:31889100-31889119 Msa0808130:intron 50.0%
TTCCGGTGACCATGACAAAC+GGG + chr5_3:31889276-31889295 None:intergenic 50.0%
GTTCCGGTGACCATGACAAA+CGG + chr5_3:31889277-31889296 None:intergenic 50.0%
CCCGCGGATAACTTTCATCT+TGG + chr5_3:31889756-31889775 None:intergenic 50.0%
TGTGCATCATCTTGTACCCG+CGG + chr5_3:31889772-31889791 None:intergenic 50.0%
CGAAGAAGCCCCGTTTGTCA+TGG - chr5_3:31889264-31889283 Msa0808130:CDS 55.0%
TCCGGTGACCATGACAAACG+GGG + chr5_3:31889275-31889294 None:intergenic 55.0%
CGAAGAAGCCCCGTTTGTCA+TGG - chr5_3:31889264-31889283 Msa0808130:CDS 55.0%
TCCGGTGACCATGACAAACG+GGG + chr5_3:31889275-31889294 None:intergenic 55.0%
GCCCCGTTTGTCATGGTCAC+CGG - chr5_3:31889271-31889290 Msa0808130:CDS 60.0%
GCCCCGTTTGTCATGGTCAC+CGG - chr5_3:31889271-31889290 Msa0808130:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 31888561 31889906 31888561 ID=Msa0808130;Name=Msa0808130
chr5_3 mRNA 31888561 31889906 31888561 ID=Msa0808130-mRNA-1;Parent=Msa0808130;Name=Msa0808130-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|1|1|5|0|313
chr5_3 exon 31889789 31889906 31889789 ID=Msa0808130-mRNA-1:exon:10555;Parent=Msa0808130-mRNA-1
chr5_3 exon 31889538 31889687 31889538 ID=Msa0808130-mRNA-1:exon:10554;Parent=Msa0808130-mRNA-1
chr5_3 exon 31889126 31889434 31889126 ID=Msa0808130-mRNA-1:exon:10553;Parent=Msa0808130-mRNA-1
chr5_3 exon 31888960 31889036 31888960 ID=Msa0808130-mRNA-1:exon:10552;Parent=Msa0808130-mRNA-1
chr5_3 exon 31888561 31888848 31888561 ID=Msa0808130-mRNA-1:exon:10551;Parent=Msa0808130-mRNA-1
chr5_3 CDS 31889789 31889906 31889789 ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
chr5_3 CDS 31889538 31889687 31889538 ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
chr5_3 CDS 31889126 31889434 31889126 ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
chr5_3 CDS 31888960 31889036 31888960 ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
chr5_3 CDS 31888561 31888848 31888561 ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
Gene Sequence

>Msa0808130

ATGGCAGTTTTCATTGACAACCCATCATTCCCTGATGGTCTCAACGTTATTGCAATTGATCATGACACCACTTTCCTTAACACCATTGAGGAGATGTGTAATCGATGCCATTATCAAGTTACCAAATGCATTACAGCTTCTGATGCCTTGAACTTGTTGGAAAGAAAGGACTGTTTTGATGTAATGTTAATTGATGCTCATATGCCAAACATGGATGCCTATGACTTTGTTCAACATGCTACTGTACAACTCAATATTCCTGTTATTATGATGTCTACTGAATATCCCACATTAAGTTCTGTTACAAAGTCTATCACATATGGGGCTTGCGAATATTGGACCAAGCCGTTGTATGAGGAACAATTCAAGATGATGTGGCAGCATGTTGTAAGAAAAGGTTTGACAGAAAATAAAAAACATGAAAATATTGGAAGCTTTGATGTTCAAGGTCATAGAAAACGGGAAAGAGAAGATGATAATGTCACAAAAGAAACAAATGCGAAGAAGCCCCGTTTGTCATGGTCACCGGAACTGCATCAACGATTTTTATGGGCTGTCAATCAACTAGGCCTTGATAAGGCAAAACCTAAAATGATTCTCAAAAAGATGGATGTTCATGGTTTGAGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGAAACTATTTGAAGAGATCGATTGATGAGAAAAAATGTGGGAAGAAAAAATCAAAGCTGAGTGATGACTTTCAAGCTCATCAGATTGACCGGAACCAACATGATGATGTTCAAGATGAATGGATTGACCAAGATGAAAGTTATCCGCGGGTACAAGATGATGCACAACAAAATTGGATGGAAATTGGCAAATCCGACAATATTCGCAACTTTTTTTCTGACATACCCAACTTGTTCCCAGATTGTGTCGTGATTAATTCATCAGTTGATTATTTCGGCTAG
Protein sequence

>Msa0808130

MAVFIDNPSFPDGLNVIAIDHDTTFLNTIEEMCNRCHYQVTKCITASDALNLLERKDCFDVMLIDAHMPNMDAYDFVQHATVQLNIPVIMMSTEYPTLSSVTKSITYGACEYWTKPLYEEQFKMMWQHVVRKGLTENKKHENIGSFDVQGHRKREREDDNVTKETNAKKPRLSWSPELHQRFLWAVNQLGLDKAKPKMILKKMDVHGLSVGHVASHLQKYRNYLKRSIDEKKCGKKKSKLSDDFQAHQIDRNQHDDVQDEWIDQDESYPRVQDDAQQNWMEIGKSDNIRNFFSDIPNLFPDCVVINSSVDYFG*