AlfalfaGEDB | Msa0808130

M. sativa cultivar zhongmu-4 / Msa0808130

KEGG: K14491
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0808130	QSD99668.1	79.931	289	56	2	13	301	39	325	6.84e-162	464

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0808130	sp\|A2XE31\|ORR21_ORYSI	37.748	302	140	6	10	264	12	312	4.26e-53	187

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0808130	A0A896WET6	79.931	289	56	2	13	301	39	325	3.27e-162	464

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TFs/TRs:

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Gene ID	Type	Classification
Msa0808130	TF	GARP-ARR-B

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0808130	MtrunA17_Chr4g0009901	75.665	263	63	1	1	263	1	262	4.98e-141	399
Msa0808130	MtrunA17_Chr4g0009851	63.245	302	108	3	1	300	1	301	1.17e-131	377
Msa0808130	MtrunA17_Chr4g0009981	61.446	249	94	2	53	300	1	248	1.47e-99	293
Msa0808130	MtrunA17_Chr1g0161431	46.774	248	100	4	10	226	11	257	1.28e-67	222
Msa0808130	MtrunA17_Chr4g0011071	67.391	138	44	1	55	192	35	171	1.96e-63	198
Msa0808130	MtrunA17_Chr5g0423031	96.629	89	3	0	1	89	1	89	6.01e-58	184
Msa0808130	MtrunA17_Chr5g0423031	83.158	95	14	1	185	277	80	174	4.08e-41	141
Msa0808130	MtrunA17_Chr2g0294911	41.445	263	126	4	10	248	29	287	2.17e-57	194
Msa0808130	MtrunA17_Chr4g0067981	43.852	244	108	4	10	226	31	272	6.97e-56	192
Msa0808130	MtrunA17_Chr3g0123131	41.224	245	115	5	10	226	13	256	3.16e-54	186

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0808130	AT2G01760.1	40.784	255	115	5	7	226	4	257	1.37e-53	180
Msa0808130	AT3G16857.1	39.924	263	111	3	10	226	33	294	1.97e-53	185
Msa0808130	AT3G16857.2	39.924	263	111	3	10	226	33	294	2.28e-53	186
Msa0808130	AT1G67710.1	40.816	245	115	5	11	226	8	251	8.84e-49	170
Msa0808130	AT2G01760.2	36.749	283	115	6	7	226	4	285	1.26e-47	165
Msa0808130	AT2G25180.1	39.004	241	122	5	10	226	13	252	9.59e-45	160
Msa0808130	AT4G31920.1	38.889	234	128	5	10	230	13	244	2.01e-42	154
Msa0808130	AT3G16857.3	38.839	224	90	3	49	226	2	224	1.14e-40	150
Msa0808130	AT5G58080.1	37.209	258	133	6	10	244	14	265	2.75e-40	149
Msa0808130	AT2G01760.3	38.342	193	84	4	68	226	1	192	5.27e-33	124

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Find 57 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATGATGCACAACAAAAT+TGG	0.295199	5_3:-31888666	Msa0808130:CDS
AAGTCATAGGCATCCATGTT+TGG	0.461471	5_3:+31889579	None:intergenic
AATTAATCACGACACAATCT+GGG	0.566059	5_3:+31888584	None:intergenic
AATTCATCAGTTGATTATTT+CGG	0.292540	5_3:-31888565	Msa0808130:CDS
ACACAATCTGGGAACAAGTT+GGG	0.508030	5_3:+31888595	None:intergenic
ACATCTCCTCAATGGTGTTA+AGG	0.419121	5_3:+31889810	None:intergenic
ACATGTTGCTAGTCATTTAC+AGG	0.286115	5_3:-31888959	Msa0808130:intron
ACCAAGATGAAAGTTATCCG+CGG	0.659940	5_3:-31888693	Msa0808130:CDS
AGCATGTTGAACAAAGTCAT+AGG	0.527015	5_3:+31889566	None:intergenic
AGGAACAATTCAAGATGATG+TGG	0.609752	5_3:-31889325	Msa0808130:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAGTAACTAACCTTGATAA+TGG	+	chr5_3:31888671-31888690	None:intergenic	30.0%
CTTTGTAACAGAACTTAATG+TGG	+	chr5_3:31889054-31889073	None:intergenic	30.0%
GTTACAAAGTCTATCACATA+TGG	-	chr5_3:31889065-31889084	Msa0808130:intron	30.0%
TACAAAGTCTATCACATATG+GGG	-	chr5_3:31889067-31889086	Msa0808130:intron	30.0%
TTGAATTGTTCCTCATACAA+CGG	+	chr5_3:31889113-31889132	None:intergenic	30.0%
ATGTTCAAGGTCATAGAAAA+CGG	-	chr5_3:31889204-31889223	Msa0808130:CDS	30.0%
ACAAAGCGAAGAAAAGAATT+AGG	+	chr5_3:31889357-31889376	None:intergenic	30.0%
TCGATTGATGAGAAAAAATG+TGG	-	chr5_3:31889643-31889662	Msa0808130:CDS	30.0%
CGATTGATGAGAAAAAATGT+GGG	-	chr5_3:31889644-31889663	Msa0808130:CDS	30.0%
AAGATGATGCACAACAAAAT+TGG	-	chr5_3:31889779-31889798	Msa0808130:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	31888561	31889906	31888561	ID=Msa0808130;Name=Msa0808130
chr5_3	mRNA	31888561	31889906	31888561	ID=Msa0808130-mRNA-1;Parent=Msa0808130;Name=Msa0808130-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0\|0\|0\|1\|1\|1\|5\|0\|313
chr5_3	exon	31889789	31889906	31889789	ID=Msa0808130-mRNA-1:exon:10555;Parent=Msa0808130-mRNA-1
chr5_3	exon	31889538	31889687	31889538	ID=Msa0808130-mRNA-1:exon:10554;Parent=Msa0808130-mRNA-1
chr5_3	exon	31889126	31889434	31889126	ID=Msa0808130-mRNA-1:exon:10553;Parent=Msa0808130-mRNA-1
chr5_3	exon	31888960	31889036	31888960	ID=Msa0808130-mRNA-1:exon:10552;Parent=Msa0808130-mRNA-1
chr5_3	exon	31888561	31888848	31888561	ID=Msa0808130-mRNA-1:exon:10551;Parent=Msa0808130-mRNA-1
chr5_3	CDS	31889789	31889906	31889789	ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
chr5_3	CDS	31889538	31889687	31889538	ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
chr5_3	CDS	31889126	31889434	31889126	ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
chr5_3	CDS	31888960	31889036	31888960	ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1
chr5_3	CDS	31888561	31888848	31888561	ID=Msa0808130-mRNA-1:cds;Parent=Msa0808130-mRNA-1

Gene Sequence

>Msa0808130

ATGGCAGTTTTCATTGACAACCCATCATTCCCTGATGGTCTCAACGTTATTGCAATTGATCATGACACCACTTTCCTTAACACCATTGAGGAGATGTGTAATCGATGCCATTATCAAGTTACCAAATGCATTACAGCTTCTGATGCCTTGAACTTGTTGGAAAGAAAGGACTGTTTTGATGTAATGTTAATTGATGCTCATATGCCAAACATGGATGCCTATGACTTTGTTCAACATGCTACTGTACAACTCAATATTCCTGTTATTATGATGTCTACTGAATATCCCACATTAAGTTCTGTTACAAAGTCTATCACATATGGGGCTTGCGAATATTGGACCAAGCCGTTGTATGAGGAACAATTCAAGATGATGTGGCAGCATGTTGTAAGAAAAGGTTTGACAGAAAATAAAAAACATGAAAATATTGGAAGCTTTGATGTTCAAGGTCATAGAAAACGGGAAAGAGAAGATGATAATGTCACAAAAGAAACAAATGCGAAGAAGCCCCGTTTGTCATGGTCACCGGAACTGCATCAACGATTTTTATGGGCTGTCAATCAACTAGGCCTTGATAAGGCAAAACCTAAAATGATTCTCAAAAAGATGGATGTTCATGGTTTGAGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGAAACTATTTGAAGAGATCGATTGATGAGAAAAAATGTGGGAAGAAAAAATCAAAGCTGAGTGATGACTTTCAAGCTCATCAGATTGACCGGAACCAACATGATGATGTTCAAGATGAATGGATTGACCAAGATGAAAGTTATCCGCGGGTACAAGATGATGCACAACAAAATTGGATGGAAATTGGCAAATCCGACAATATTCGCAACTTTTTTTCTGACATACCCAACTTGTTCCCAGATTGTGTCGTGATTAATTCATCAGTTGATTATTTCGGCTAG

Protein sequence

>Msa0808130

MAVFIDNPSFPDGLNVIAIDHDTTFLNTIEEMCNRCHYQVTKCITASDALNLLERKDCFDVMLIDAHMPNMDAYDFVQHATVQLNIPVIMMSTEYPTLSSVTKSITYGACEYWTKPLYEEQFKMMWQHVVRKGLTENKKHENIGSFDVQGHRKREREDDNVTKETNAKKPRLSWSPELHQRFLWAVNQLGLDKAKPKMILKKMDVHGLSVGHVASHLQKYRNYLKRSIDEKKCGKKKSKLSDDFQAHQIDRNQHDDVQDEWIDQDESYPRVQDDAQQNWMEIGKSDNIRNFFSDIPNLFPDCVVINSSVDYFG*