AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0809220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0809220 MtrunA17_Chr5g0425261 73.897 272 67 3 14 281 70 341 3.04e-133 382
Msa0809220 MtrunA17_Chr5g0424801 74.265 272 66 3 14 281 70 341 4.41e-133 382
Msa0809220 MtrunA17_Chr5g0424741 52.751 309 105 6 14 282 70 377 4.34e-94 287
Msa0809220 MtrunA17_Chr5g0424931 52.685 298 98 9 14 275 77 367 6.27e-86 266
Msa0809220 MtrunA17_Chr1g0178011 53.585 265 102 9 25 275 81 338 2.89e-73 232
Msa0809220 MtrunA17_Chr5g0417281 44.275 262 123 9 26 275 81 331 6.96e-60 199
Msa0809220 MtrunA17_Chr2g0289221 46.964 247 98 11 26 255 91 321 5.72e-56 184
Msa0809220 MtrunA17_Chr2g0277461 45.455 264 118 11 26 275 85 336 1.40e-55 186
Msa0809220 MtrunA17_Chr5g0424791 75.833 120 27 1 25 142 32 151 2.89e-54 173
Msa0809220 MtrunA17_Chr5g0436761 42.910 268 124 9 25 275 81 336 1.39e-53 181
Msa0809220 MtrunA17_Chr5g0417211 46.332 259 104 12 26 265 82 324 7.01e-52 175
Msa0809220 MtrunA17_Chr2g0277531 38.583 254 129 11 14 255 80 318 3.39e-42 147
Msa0809220 MtrunA17_Chr5g0425651 63.393 112 39 2 168 278 1 111 1.70e-41 139
Msa0809220 MtrunA17_Chr3g0115271 33.945 327 145 11 11 275 63 380 3.67e-41 150
Msa0809220 MtrunA17_Chr1g0181211 33.677 291 133 11 47 281 120 406 8.14e-33 127
Msa0809220 MtrunA17_Chr2g0326761 30.592 304 153 9 25 275 77 375 1.16e-32 127
Msa0809220 MtrunA17_Chr4g0028971 33.101 287 127 11 54 280 107 388 2.61e-32 125
Msa0809220 MtrunA17_Chr1g0199681 32.734 278 131 9 54 280 191 463 2.95e-31 122
Msa0809220 MtrunA17_Chr7g0264171 29.592 294 138 11 51 281 126 413 4.52e-29 116
Msa0809220 MtrunA17_Chr5g0425661 47.101 138 58 5 14 137 92 228 1.53e-26 104
Msa0809220 MtrunA17_Chr7g0264161 31.364 220 131 8 51 255 87 301 7.79e-24 98.6
Msa0809220 MtrunA17_Chr2g0276781 28.881 277 155 9 25 275 94 354 8.88e-22 95.1
Msa0809220 MtrunA17_Chr7g0239061 27.715 267 170 8 25 275 85 344 2.38e-21 94.0
Msa0809220 MtrunA17_Chr4g0009751 28.030 264 173 7 25 275 90 349 5.98e-21 92.8
Msa0809220 MtrunA17_Chr2g0285461 29.391 279 153 12 25 275 95 357 1.87e-19 88.6
Msa0809220 MtrunA17_Chr2g0282961 26.293 232 157 6 56 275 158 387 3.33e-19 87.8
Msa0809220 MtrunA17_Chr8g0376771 28.159 277 159 11 25 275 94 356 2.06e-18 85.5
Msa0809220 MtrunA17_Chr8g0385791 26.423 246 157 7 52 280 158 396 4.85e-18 84.3
Msa0809220 MtrunA17_Chr5g0433301 28.520 277 158 11 25 275 96 358 2.35e-17 82.4
Msa0809220 MtrunA17_Chr4g0070181 27.542 236 159 5 56 280 126 360 4.61e-17 81.6
Msa0809220 MtrunA17_Chr8g0346711 27.925 265 167 8 27 275 102 358 5.21e-17 81.3
Msa0809220 MtrunA17_Chr3g0109391 27.437 277 161 10 25 275 98 360 5.61e-17 81.3
Msa0809220 MtrunA17_Chr2g0301251 27.615 239 155 7 56 280 126 360 7.13e-17 80.9
Msa0809220 MtrunA17_Chr4g0046371 25.862 232 157 7 56 275 148 376 7.75e-17 80.5
Msa0809220 MtrunA17_Chr4g0029671 29.237 236 146 9 56 275 166 396 2.15e-16 79.3
Msa0809220 MtrunA17_Chr1g0156321 29.412 238 143 10 56 275 146 376 2.47e-15 76.3
Msa0809220 MtrunA17_Chr8g0389081 27.542 236 150 9 56 275 136 366 1.12e-13 71.2
Msa0809220 MtrunA17_Chr2g0326281 27.338 278 157 11 34 275 143 411 1.23e-13 71.2
Msa0809220 MtrunA17_Chr8g0356211 24.034 233 148 7 69 284 9 229 1.24e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0809220 AT4G30080.1 34.202 307 139 12 25 275 84 383 1.34e-35 135
Msa0809220 AT1G77850.1 31.438 299 153 12 25 275 83 377 2.00e-34 131
Msa0809220 AT1G77850.2 31.438 299 153 12 25 275 83 377 2.13e-34 131
Msa0809220 AT2G28350.2 31.419 296 142 13 41 280 97 387 6.43e-30 119
Msa0809220 AT2G28350.1 31.419 296 142 13 41 280 97 387 6.67e-30 119
Msa0809220 AT2G46530.3 29.787 282 169 10 14 275 99 371 6.58e-25 104
Msa0809220 AT2G46530.1 29.787 282 169 10 14 275 78 350 8.13e-25 104
Msa0809220 AT2G46530.2 30.709 254 146 10 38 275 24 263 1.78e-23 100
Msa0809220 AT3G61830.1 29.412 272 157 12 25 275 97 354 1.89e-23 100
Msa0809220 AT3G61830.2 29.412 272 157 12 25 275 46 303 2.06e-23 100
Msa0809220 AT1G59750.1 29.740 269 163 9 25 275 93 353 6.43e-22 96.3
Msa0809220 AT1G59750.3 29.740 269 163 9 25 275 93 353 6.43e-22 96.3
Msa0809220 AT1G59750.2 29.740 269 163 9 25 275 93 353 7.12e-22 95.9
Msa0809220 AT1G59750.4 29.740 269 163 9 25 275 93 353 7.31e-22 95.9
Msa0809220 AT1G34390.1 28.920 287 156 11 25 284 98 363 7.75e-21 92.8
Msa0809220 AT1G35520.1 29.137 278 161 12 25 280 98 361 4.07e-20 90.9
Msa0809220 AT1G34410.1 29.225 284 161 13 25 284 98 365 1.89e-19 88.6
Msa0809220 AT4G23980.1 26.978 278 179 9 25 284 84 355 2.22e-19 88.6
Msa0809220 AT4G23980.2 26.978 278 179 9 25 284 84 355 2.36e-19 88.6
Msa0809220 AT1G35240.1 28.723 282 165 12 25 284 98 365 5.23e-19 87.4
Msa0809220 AT1G30330.3 28.758 306 166 13 5 275 71 359 5.62e-19 87.4
Msa0809220 AT1G30330.2 28.758 306 166 13 5 275 71 359 5.62e-19 87.4
Msa0809220 AT1G30330.1 28.758 306 166 13 5 275 69 357 5.83e-19 87.4
Msa0809220 AT1G34310.1 28.975 283 163 12 25 284 98 365 7.20e-19 87.0
Msa0809220 AT5G37020.2 28.315 279 156 12 25 275 94 356 1.52e-18 86.3
Msa0809220 AT2G33860.2 28.870 239 142 9 56 275 2 231 1.66e-18 85.5
Msa0809220 AT2G33860.1 28.870 239 142 9 56 275 159 388 1.73e-18 85.9
Msa0809220 AT5G37020.1 28.315 279 156 12 25 275 94 356 1.99e-18 85.9
Msa0809220 AT5G37020.3 28.309 272 154 11 29 275 6 261 7.09e-18 84.3
Msa0809220 AT5G62000.4 25.442 283 168 9 25 280 132 398 1.94e-17 82.8
Msa0809220 AT5G62000.2 25.442 283 168 9 25 280 132 398 1.96e-17 82.8
Msa0809220 AT5G62000.5 25.442 283 168 9 25 280 132 398 1.96e-17 82.8
Msa0809220 AT5G62000.3 25.442 283 168 9 25 280 132 398 1.96e-17 82.8
Msa0809220 AT5G62000.1 25.442 283 168 9 25 280 132 398 1.96e-17 82.8
Msa0809220 AT1G35540.1 27.239 268 165 10 25 275 98 352 2.49e-17 82.4
Msa0809220 AT5G60450.1 27.165 254 155 10 44 275 160 405 2.29e-15 76.6
Msa0809220 AT5G60450.2 27.165 254 155 10 44 275 136 381 2.65e-15 76.6
Msa0809220 AT5G20730.3 27.848 237 158 6 56 280 127 362 6.57e-15 75.5
Msa0809220 AT5G20730.1 27.848 237 158 6 56 280 127 362 7.57e-15 75.5
Msa0809220 AT5G20730.2 28.270 237 156 7 56 280 127 361 1.32e-14 74.7
Msa0809220 AT1G19850.2 28.448 232 153 6 56 275 147 377 1.98e-14 73.9
Msa0809220 AT1G19850.1 28.448 232 153 6 56 275 158 388 2.01e-14 73.9
Msa0809220 AT1G19220.1 26.582 237 160 7 56 280 126 360 1.12e-13 71.6

Find 61 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAAAACAACAGAATCTT+TGG 0.228767 5_3:+35284883 None:intergenic
TGTCTCCATCATCGTCTTCT+TGG 0.236516 5_3:+35285128 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.254844 5_3:-35284532 Msa0809220:CDS
ATGAAGATTGGTTGGGAATC+TGG 0.311617 5_3:-35284647 Msa0809220:CDS
CGTAGGTGCAACCCCATTCT+TGG 0.311998 5_3:+35284966 None:intergenic
GATCTTTCCCAAGCTTGATT+TGG 0.314401 5_3:-35285026 Msa0809220:CDS
CTTTCACCCGCTTCAGCTGC+AGG 0.324014 5_3:+35284809 None:intergenic
ACCTCAAGAATGCGCCAATT+AGG 0.328543 5_3:+35284518 None:intergenic
TGTTCCCTTAGGCTGATAAT+AGG 0.341760 5_3:+35284574 None:intergenic
CCTCAAGAATGCGCCAATTA+GGG 0.362876 5_3:+35284519 None:intergenic
TCCATCATCGTCTTCTTGGC+CGG 0.379595 5_3:+35285132 None:intergenic
AGATTCCCAACCAATCTTCA+TGG 0.412679 5_3:+35284649 None:intergenic
GCCGGGAACTACAGGAGGAG+GGG 0.417463 5_3:+35285150 None:intergenic
CTTGGTCAGTTACGGGAGTC+AGG 0.426329 5_3:+35285176 None:intergenic
TGGCCGGGAACTACAGGAGG+AGG 0.428083 5_3:+35285148 None:intergenic
CGACCTTAAAAGCCAAGAAT+GGG 0.432454 5_3:-35284978 Msa0809220:CDS
TCTGCTGGAAAGATATTAGT+TGG 0.452740 5_3:-35284854 Msa0809220:CDS
GACTCGTCCAAATCAAGCTT+GGG 0.456946 5_3:+35285019 None:intergenic
CCGCACTCTCAGTTAAATTC+CGG 0.457010 5_3:+35284783 None:intergenic
TCTTCTTGGCCGGGAACTAC+AGG 0.462528 5_3:+35285142 None:intergenic
CCATCATCGTCTTCTTGGCC+GGG 0.465753 5_3:+35285133 None:intergenic
CCGGGAACTACAGGAGGAGG+GGG 0.475602 5_3:+35285151 None:intergenic
AAAGACCTATTATCAGCCTA+AGG 0.480294 5_3:-35284579 Msa0809220:CDS
AACTTCCCTGCAGCTGAAGC+GGG 0.481484 5_3:-35284815 Msa0809220:CDS
TTATCATAGGTTAACTGGGA+TGG 0.485250 5_3:-35283905 Msa0809220:intron
TCACACAAATTCCTAGCTGT+TGG 0.489140 5_3:+35284704 None:intergenic
AGACTCGTCCAAATCAAGCT+TGG 0.490804 5_3:+35285018 None:intergenic
GTAGATGATGCCATGAAGAT+TGG 0.497274 5_3:-35284659 Msa0809220:CDS
CCCTAATTGGCGCATTCTTG+AGG 0.497342 5_3:-35284519 Msa0809220:intron
GCAGCTGAAGCGGGTGAAAG+AGG 0.499828 5_3:-35284806 Msa0809220:CDS
CCGACCTTAAAAGCCAAGAA+TGG 0.501034 5_3:-35284979 Msa0809220:CDS
ATACAAACATAGTCAATCCT+TGG 0.507379 5_3:-35283865 Msa0809220:CDS
AGATATTAGTTGGAATTCGC+AGG 0.508600 5_3:-35284844 Msa0809220:CDS
TTTGAATACAGTTGAATTGG+CGG 0.515164 5_3:-35284759 Msa0809220:CDS
CTGAAGCGGGTGAAAGAGGC+CGG 0.516670 5_3:-35284802 Msa0809220:CDS
ATGGTTCTTGTGGAAGCAGT+AGG 0.527864 5_3:+35285245 None:intergenic
ACAGTAGAGATTGTTCCCTT+AGG 0.533124 5_3:+35284563 None:intergenic
TGGTTCTTGTGGAAGCAGTA+GGG 0.540249 5_3:+35285246 None:intergenic
TAACTTCCCTGCAGCTGAAG+CGG 0.546068 5_3:-35284816 Msa0809220:CDS
ATGATGCCATGAAGATTGGT+TGG 0.554453 5_3:-35284655 Msa0809220:CDS
TTGCAAGCTTCAAGCTGCCA+AGG 0.562587 5_3:+35283848 None:intergenic
CCCGGCCAAGAAGACGATGA+TGG 0.562611 5_3:-35285133 Msa0809220:CDS
TGATGCCATGAAGATTGGTT+GGG 0.569149 5_3:-35284654 Msa0809220:CDS
TTTCACCCGCTTCAGCTGCA+GGG 0.570879 5_3:+35284810 None:intergenic
ACGATCAAGAATGGTTCTTG+TGG 0.577736 5_3:+35285235 None:intergenic
TGAATTGGCGGACAAGAACA+TGG 0.578093 5_3:-35284747 Msa0809220:CDS
GGAGCAGTTGAGACGATGCA+TGG 0.578742 5_3:+35285197 None:intergenic
GGCCGGGAACTACAGGAGGA+GGG 0.579671 5_3:+35285149 None:intergenic
TGGACGGTAACGATCAAGAA+TGG 0.583957 5_3:+35285226 None:intergenic
TTGTGTATTATCCAACAGCT+AGG 0.593766 5_3:-35284715 Msa0809220:CDS
GAGACGATGCATGGAATGGA+TGG 0.598558 5_3:+35285206 None:intergenic
ACAGTAGACTACGTACTGGC+TGG 0.603214 5_3:-35284937 Msa0809220:CDS
GGTTCTTGTGGAAGCAGTAG+GGG 0.606289 5_3:+35285247 None:intergenic
CCGGAATTTAACTGAGAGTG+CGG 0.606695 5_3:-35284783 Msa0809220:CDS
AAGACCTATTATCAGCCTAA+GGG 0.614032 5_3:-35284578 Msa0809220:CDS
CGATGCATGGAATGGATGGA+CGG 0.620709 5_3:+35285210 None:intergenic
AGTTGAGACGATGCATGGAA+TGG 0.633630 5_3:+35285202 None:intergenic
TTGTGTGAGAGTCTTGACAT+AGG 0.637006 5_3:+35285096 None:intergenic
TCTTGGCCGGGAACTACAGG+AGG 0.657068 5_3:+35285145 None:intergenic
ATCAAGTTAGAGCATTCGAT+AGG 0.670484 5_3:+35285046 None:intergenic
GACCTTAAAAGCCAAGAATG+GGG 0.680749 5_3:-35284977 Msa0809220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAATAGATATATAAAATT+TGG - chr5_3:35284703-35284722 Msa0809220:CDS 10.0%
!! TCAAACAAATCATAAAATTA+TGG + chr5_3:35284629-35284648 None:intergenic 15.0%
!!! TTCTTACCATTTTTTATCAT+AGG - chr5_3:35285140-35285159 Msa0809220:CDS 20.0%
!! AGTTAACCTATGATAAAAAA+TGG + chr5_3:35285149-35285168 None:intergenic 20.0%
!!! ATTTTTTATCATAGGTTAAC+TGG - chr5_3:35285148-35285167 Msa0809220:CDS 20.0%
!!! TTTTTTATCATAGGTTAACT+GGG - chr5_3:35285149-35285168 Msa0809220:CDS 20.0%
!!! TTTGTATTTTGCAAGATTTT+AGG + chr5_3:35285180-35285199 None:intergenic 20.0%
!!! CTCAATTTTAGAATTGCATT+TGG + chr5_3:35284789-35284808 None:intergenic 25.0%
CATGAAAACAACAGAATCTT+TGG + chr5_3:35284178-35284197 None:intergenic 30.0%
!!! GGTTTTGAATACAGTTGAAT+TGG - chr5_3:35284296-35284315 Msa0809220:intron 30.0%
TTTGAATACAGTTGAATTGG+CGG - chr5_3:35284299-35284318 Msa0809220:intron 30.0%
! GAATTTGTGTGATTTTGTGA+TGG - chr5_3:35284365-35284384 Msa0809220:intron 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - chr5_3:35284526-35284545 Msa0809220:CDS 30.0%
ACATAGTAGATTCTTCTGTT+TGG - chr5_3:35284728-35284747 Msa0809220:CDS 30.0%
! CATTTGGAACTTATCCTATA+CGG + chr5_3:35284773-35284792 None:intergenic 30.0%
AACAAGGGAATTAAGAATGT+TGG + chr5_3:35284838-35284857 None:intergenic 30.0%
TACAATGAGAAATGCTTAGA+AGG + chr5_3:35284999-35285018 None:intergenic 30.0%
ATACAAACATAGTCAATCCT+TGG - chr5_3:35285193-35285212 Msa0809220:CDS 30.0%
!!! TGCAAAAGTTTTGAATGACA+GGG - chr5_3:35285239-35285258 Msa0809220:CDS 30.0%
! ATCAAGTTAGAGCATTCGAT+AGG + chr5_3:35284015-35284034 None:intergenic 35.0%
!! TAGTCTACTGTTTTTCACGT+AGG + chr5_3:35284112-35284131 None:intergenic 35.0%
AAAAACAGTAGACTACGTAC+TGG - chr5_3:35284117-35284136 Msa0809220:intron 35.0%
! GTTTTCATGAAAAACTCTGC+TGG - chr5_3:35284189-35284208 Msa0809220:intron 35.0%
TCTGCTGGAAAGATATTAGT+TGG - chr5_3:35284204-35284223 Msa0809220:intron 35.0%
AGATATTAGTTGGAATTCGC+AGG - chr5_3:35284214-35284233 Msa0809220:intron 35.0%
TTGTGTATTATCCAACAGCT+AGG - chr5_3:35284343-35284362 Msa0809220:intron 35.0%
!!! TGATTTTGTGATGGATGCTA+AGG - chr5_3:35284374-35284393 Msa0809220:intron 35.0%
AAAGACCTATTATCAGCCTA+AGG - chr5_3:35284479-35284498 Msa0809220:intron 35.0%
AAGACCTATTATCAGCCTAA+GGG - chr5_3:35284480-35284499 Msa0809220:intron 35.0%
GGAATTAAGAATGTTGGAGA+TGG + chr5_3:35284832-35284851 None:intergenic 35.0%
TCTATCAAGTGCAAGAACAA+GGG + chr5_3:35284853-35284872 None:intergenic 35.0%
TTATCATAGGTTAACTGGGA+TGG - chr5_3:35285153-35285172 Msa0809220:CDS 35.0%
! CTGTCATTCAAAACTTTTGC+AGG + chr5_3:35285240-35285259 None:intergenic 35.0%
!!! CTGCAAAAGTTTTGAATGAC+AGG - chr5_3:35285238-35285257 Msa0809220:CDS 35.0%
ACGATCAAGAATGGTTCTTG+TGG + chr5_3:35283826-35283845 None:intergenic 40.0%
TTGTGTGAGAGTCTTGACAT+AGG + chr5_3:35283965-35283984 None:intergenic 40.0%
GATCTTTCCCAAGCTTGATT+TGG - chr5_3:35284032-35284051 Msa0809220:intron 40.0%
!!! CCATTCTTGGCTTTTAAGGT+CGG + chr5_3:35284082-35284101 None:intergenic 40.0%
CGACCTTAAAAGCCAAGAAT+GGG - chr5_3:35284080-35284099 Msa0809220:intron 40.0%
GACCTTAAAAGCCAAGAATG+GGG - chr5_3:35284081-35284100 Msa0809220:intron 40.0%
!! AACCCCATTCTTGGCTTTTA+AGG + chr5_3:35284086-35284105 None:intergenic 40.0%
TCACACAAATTCCTAGCTGT+TGG + chr5_3:35284357-35284376 None:intergenic 40.0%
GTAGATGATGCCATGAAGAT+TGG - chr5_3:35284399-35284418 Msa0809220:intron 40.0%
ATGATGCCATGAAGATTGGT+TGG - chr5_3:35284403-35284422 Msa0809220:intron 40.0%
! TGATGCCATGAAGATTGGTT+GGG - chr5_3:35284404-35284423 Msa0809220:intron 40.0%
AGATTCCCAACCAATCTTCA+TGG + chr5_3:35284412-35284431 None:intergenic 40.0%
! ATGAAGATTGGTTGGGAATC+TGG - chr5_3:35284411-35284430 Msa0809220:intron 40.0%
TGTTCCCTTAGGCTGATAAT+AGG + chr5_3:35284487-35284506 None:intergenic 40.0%
ACAGTAGAGATTGTTCCCTT+AGG + chr5_3:35284498-35284517 None:intergenic 40.0%
GTCTATCAAGTGCAAGAACA+AGG + chr5_3:35284854-35284873 None:intergenic 40.0%
AAACACTCCTGTTGCTAAGT+AGG - chr5_3:35285018-35285037 Msa0809220:CDS 40.0%
TCAAGATCCTACTTAGCAAC+AGG + chr5_3:35285028-35285047 None:intergenic 40.0%
! TTTTGAATGACAGGGTTGAG+TGG - chr5_3:35285247-35285266 Msa0809220:CDS 40.0%
TGGTTCTTGTGGAAGCAGTA+GGG + chr5_3:35283815-35283834 None:intergenic 45.0%
ATGGTTCTTGTGGAAGCAGT+AGG + chr5_3:35283816-35283835 None:intergenic 45.0%
TGGACGGTAACGATCAAGAA+TGG + chr5_3:35283835-35283854 None:intergenic 45.0%
AGTTGAGACGATGCATGGAA+TGG + chr5_3:35283859-35283878 None:intergenic 45.0%
TGTCTCCATCATCGTCTTCT+TGG + chr5_3:35283933-35283952 None:intergenic 45.0%
GACTCGTCCAAATCAAGCTT+GGG + chr5_3:35284042-35284061 None:intergenic 45.0%
AGACTCGTCCAAATCAAGCT+TGG + chr5_3:35284043-35284062 None:intergenic 45.0%
CCGACCTTAAAAGCCAAGAA+TGG - chr5_3:35284079-35284098 Msa0809220:intron 45.0%
CCGCACTCTCAGTTAAATTC+CGG + chr5_3:35284278-35284297 None:intergenic 45.0%
CCGGAATTTAACTGAGAGTG+CGG - chr5_3:35284275-35284294 Msa0809220:intron 45.0%
! TGAATTGGCGGACAAGAACA+TGG - chr5_3:35284311-35284330 Msa0809220:intron 45.0%
CCTCAAGAATGCGCCAATTA+GGG + chr5_3:35284542-35284561 None:intergenic 45.0%
ACCTCAAGAATGCGCCAATT+AGG + chr5_3:35284543-35284562 None:intergenic 45.0%
!! AAGCTAGCTTTGTGCCGTAT+AGG - chr5_3:35284756-35284775 Msa0809220:CDS 45.0%
GGTTCTTGTGGAAGCAGTAG+GGG + chr5_3:35283814-35283833 None:intergenic 50.0%
CGATGCATGGAATGGATGGA+CGG + chr5_3:35283851-35283870 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG + chr5_3:35283855-35283874 None:intergenic 50.0%
AGGGGGTTCTTGGTCAGTTA+CGG + chr5_3:35283893-35283912 None:intergenic 50.0%
TCCATCATCGTCTTCTTGGC+CGG + chr5_3:35283929-35283948 None:intergenic 50.0%
! ACAGTAGACTACGTACTGGC+TGG - chr5_3:35284121-35284140 Msa0809220:intron 50.0%
TAACTTCCCTGCAGCTGAAG+CGG - chr5_3:35284242-35284261 Msa0809220:intron 50.0%
CCCTAATTGGCGCATTCTTG+AGG - chr5_3:35284539-35284558 Msa0809220:CDS 50.0%
TTGCAAGCTTCAAGCTGCCA+AGG + chr5_3:35285213-35285232 None:intergenic 50.0%
GGAGCAGTTGAGACGATGCA+TGG + chr5_3:35283864-35283883 None:intergenic 55.0%
CTTGGTCAGTTACGGGAGTC+AGG + chr5_3:35283885-35283904 None:intergenic 55.0%
GGGGGTTCTTGGTCAGTTAC+GGG + chr5_3:35283892-35283911 None:intergenic 55.0%
TCTTCTTGGCCGGGAACTAC+AGG + chr5_3:35283919-35283938 None:intergenic 55.0%
CCATCATCGTCTTCTTGGCC+GGG + chr5_3:35283928-35283947 None:intergenic 55.0%
CGTAGGTGCAACCCCATTCT+TGG + chr5_3:35284095-35284114 None:intergenic 55.0%
AACTTCCCTGCAGCTGAAGC+GGG - chr5_3:35284243-35284262 Msa0809220:intron 55.0%
TTTCACCCGCTTCAGCTGCA+GGG + chr5_3:35284251-35284270 None:intergenic 55.0%
CTACAGGAGGAGGGGGTTCT+TGG + chr5_3:35283903-35283922 None:intergenic 60.0%
TCTTGGCCGGGAACTACAGG+AGG + chr5_3:35283916-35283935 None:intergenic 60.0%
CCCGGCCAAGAAGACGATGA+TGG - chr5_3:35283925-35283944 Msa0809220:intron 60.0%
CTTTCACCCGCTTCAGCTGC+AGG + chr5_3:35284252-35284271 None:intergenic 60.0%
GCAGCTGAAGCGGGTGAAAG+AGG - chr5_3:35284252-35284271 Msa0809220:intron 60.0%
CTGAAGCGGGTGAAAGAGGC+CGG - chr5_3:35284256-35284275 Msa0809220:intron 60.0%
CCGGGAACTACAGGAGGAGG+GGG + chr5_3:35283910-35283929 None:intergenic 65.0%
CCCCCTCCTCCTGTAGTTCC+CGG - chr5_3:35283907-35283926 Msa0809220:intron 65.0%
GCCGGGAACTACAGGAGGAG+GGG + chr5_3:35283911-35283930 None:intergenic 65.0%
GGCCGGGAACTACAGGAGGA+GGG + chr5_3:35283912-35283931 None:intergenic 65.0%
TGGCCGGGAACTACAGGAGG+AGG + chr5_3:35283913-35283932 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 35283799 35285281 35283799 ID=Msa0809220;Name=Msa0809220
chr5_3 mRNA 35283799 35285281 35283799 ID=Msa0809220-mRNA-1;Parent=Msa0809220;Name=Msa0809220-mRNA-1;_AED=0.27;_eAED=0.28;_QI=0|0|0|1|1|1|2|0|293
chr5_3 exon 35284520 35285281 35284520 ID=Msa0809220-mRNA-1:exon:11140;Parent=Msa0809220-mRNA-1
chr5_3 exon 35283799 35283918 35283799 ID=Msa0809220-mRNA-1:exon:11139;Parent=Msa0809220-mRNA-1
chr5_3 CDS 35284520 35285281 35284520 ID=Msa0809220-mRNA-1:cds;Parent=Msa0809220-mRNA-1
chr5_3 CDS 35283799 35283918 35283799 ID=Msa0809220-mRNA-1:cds;Parent=Msa0809220-mRNA-1
Gene Sequence

>Msa0809220

ATGCATGTTCTTCCCCTACTGCTTCCACAAGAACCATTCTTGATCGTTACCGTCCATCCATTCCATGCATCGTCTCAACTGCTCCTGACTCCCGTAACTGACCAAGAACCCCCTCCTCCTGTAGTTCCCGGCCAAGAAGACGATGATGGAGACAATCTTGTTTCCTATGTCAAGACTCTCACACAATCTGATTGTGCAAGAGTATTATGCGTACCTATCGAATGCTCTAACTTGATCTTTCCCAAGCTTGATTTGGACGAGTCTCAGAGCATCACTGTCACCGACCTTAAAAGCCAAGAATGGGGTTGCACCTACGTGAAAAACAGTAGACTACGTACTGGCTGGTCTCACTTTGTGAAAGAGAAGAAACTCGTAGCCAAAGATTCTGTTGTTTTCATGAAAAACTCTGCTGGAAAGATATTAGTTGGAATTCGCAGGAACACTAACTTCCCTGCAGCTGAAGCGGGTGAAAGAGGCCGGAATTTAACTGAGAGTGCGGTTTTGAATACAGTTGAATTGGCGGACAAGAACATGGAGTTTGAAGTTGTGTATTATCCAACAGCTAGGAATTTGTGTGATTTTGTGATGGATGCTAAGGTTGTAGATGATGCCATGAAGATTGGTTGGGAATCTGGAATGAGAGTCAAACTTACGTTGAAGAAAGATGAATCTTCAAATTCAAAGACCTATTATCAGCCTAAGGGAACAATCTCTACTGTCTTTAATTATTCTTCTAATGTCCCTAATTGGCGCATTCTTGAGGTTAACTGGGATGGACCTAAAATCTTGCAAAATACAAACATAGTCAATCCTTGGCAGCTTGAAGCTTGCAACATACCTGCAAAAGTTTTGAATGACAGGGTTGAGTGGTACTTTGTCTGA
Protein sequence

>Msa0809220

MHVLPLLLPQEPFLIVTVHPFHASSQLLLTPVTDQEPPPPVVPGQEDDDGDNLVSYVKTLTQSDCARVLCVPIECSNLIFPKLDLDESQSITVTDLKSQEWGCTYVKNSRLRTGWSHFVKEKKLVAKDSVVFMKNSAGKILVGIRRNTNFPAAEAGERGRNLTESAVLNTVELADKNMEFEVVYYPTARNLCDFVMDAKVVDDAMKIGWESGMRVKLTLKKDESSNSKTYYQPKGTISTVFNYSSNVPNWRILEVNWDGPKILQNTNIVNPWQLEACNIPAKVLNDRVEWYFV*