AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0810610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0810610 MtrunA17_Chr5g0427001 50.000 122 44 3 1 105 1 122 2.24e-32 114
Msa0810610 MtrunA17_Chr1g0161721 40.972 144 46 2 1 105 490 633 6.91e-26 100
Msa0810610 MtrunA17_Chr1g0161711 64.789 71 25 0 35 105 556 626 1.64e-25 99.4
Msa0810610 MtrunA17_Chr1g0161701 58.750 80 29 1 30 105 552 631 9.32e-25 97.4
Msa0810610 MtrunA17_Chr8g0392271 60.000 70 27 1 36 105 562 630 1.92e-20 85.1
Msa0810610 MtrunA17_Chr3g0121971 57.971 69 29 0 37 105 570 638 2.58e-20 84.7
Msa0810610 MtrunA17_Chr7g0267441 47.561 82 43 0 31 112 575 656 7.01e-20 83.6
Msa0810610 MtrunA17_Chr5g0434141 57.746 71 30 0 35 105 575 645 2.14e-19 82.0
Msa0810610 MtrunA17_Chr4g0025601 41.053 95 56 0 11 105 590 684 1.29e-18 79.7
Msa0810610 MtrunA17_Chr2g0321921 52.174 69 33 0 37 105 590 658 1.36e-18 79.7
Msa0810610 MtrunA17_Chr8g0377951 55.072 69 31 0 37 105 554 622 1.37e-18 79.7
Msa0810610 MtrunA17_Chr7g0274261 50.000 64 30 1 42 105 517 578 2.82e-15 70.1
Msa0810610 MtrunA17_Chr8g0354401 56.061 66 27 2 40 105 44 107 3.37e-15 65.9
Msa0810610 MtrunA17_Chr1g0190581 38.318 107 59 3 3 105 470 573 3.70e-15 70.1
Msa0810610 MtrunA17_Chr8g0354311 51.515 66 30 1 40 105 27 90 1.27e-14 65.1
Msa0810610 MtrunA17_Chr3g0082941 44.186 86 45 2 20 105 143 225 1.93e-14 67.8
Msa0810610 MtrunA17_Chr8g0354351 50.746 67 31 1 39 105 321 385 3.87e-14 67.0
Msa0810610 MtrunA17_Chr1g0148461 43.939 66 35 1 45 110 430 493 9.75e-14 65.9
Msa0810610 MtrunA17_Chr8g0354431 50.000 72 34 2 39 110 330 399 1.31e-13 65.5
Msa0810610 MtrunA17_Chr8g0354421 48.485 66 32 1 40 105 352 415 4.91e-13 63.9
Msa0810610 MtrunA17_Chr8g0361251 46.753 77 36 2 32 105 484 558 5.13e-13 63.9
Msa0810610 MtrunA17_Chr6g0475111 43.478 69 37 1 42 110 578 644 7.48e-13 63.5
Msa0810610 MtrunA17_Chr4g0061081 42.857 84 44 2 22 105 499 578 1.22e-12 62.8
Msa0810610 MtrunA17_Chr3g0081011 40.659 91 51 2 15 105 304 391 1.55e-12 62.4
Msa0810610 MtrunA17_Chr1g0148501 42.424 66 36 1 45 110 423 486 1.65e-12 62.4
Msa0810610 MtrunA17_Chr7g0236561 48.529 68 33 1 38 105 492 557 2.02e-12 62.0
Msa0810610 MtrunA17_Chr2g0327671 46.970 66 33 1 40 105 491 554 2.06e-12 62.0
Msa0810610 MtrunA17_Chr8g0354331 47.761 67 33 1 39 105 88 152 2.09e-12 62.0
Msa0810610 MtrunA17_Chr8g0354441 46.970 66 33 1 40 105 339 402 2.46e-12 62.0
Msa0810610 MtrunA17_Chr6g0475001 43.243 74 36 2 41 110 558 629 2.93e-12 61.6
Msa0810610 MtrunA17_Chr6g0475131 50.000 62 29 1 44 105 2 61 3.00e-12 61.2
Msa0810610 MtrunA17_Chr3g0081001 45.833 72 37 1 39 110 331 400 3.07e-12 61.6
Msa0810610 MtrunA17_Chr6g0474871 43.243 74 36 2 41 110 551 622 3.08e-12 61.6
Msa0810610 MtrunA17_Chr8g0347571 43.243 74 36 2 41 110 556 627 3.30e-12 61.6
Msa0810610 MtrunA17_Chr4g0030441 46.970 66 33 1 40 105 488 551 3.42e-12 61.6
Msa0810610 MtrunA17_Chr8g0354271 46.970 66 33 1 40 105 324 387 3.45e-12 61.6
Msa0810610 MtrunA17_Chr1g0206561 38.961 77 45 1 34 110 312 386 3.91e-12 61.2
Msa0810610 MtrunA17_Chr1g0206551 43.478 69 37 1 42 110 26 92 5.13e-12 60.8
Msa0810610 MtrunA17_Chr1g0194541 42.105 76 42 1 31 106 270 343 6.03e-12 60.8
Msa0810610 MtrunA17_Chr2g0320111 44.118 68 36 1 39 106 79 144 6.11e-12 60.5
Msa0810610 MtrunA17_Chr6g0474901 43.243 74 36 2 41 110 552 623 7.04e-12 60.5
Msa0810610 MtrunA17_Chr1g0206261 43.478 69 37 1 42 110 80 146 7.10e-12 60.5
Msa0810610 MtrunA17_Chr6g0474811 43.243 74 36 2 41 110 559 630 7.33e-12 60.5
Msa0810610 MtrunA17_Chr1g0206461 33.981 103 66 1 3 105 285 385 7.94e-12 60.5
Msa0810610 MtrunA17_Chr1g0201611 43.243 74 40 1 37 110 72 143 8.43e-12 60.1
Msa0810610 MtrunA17_Chr7g0245131 51.613 62 27 2 45 105 528 587 9.25e-12 60.1
Msa0810610 MtrunA17_Chr2g0299041 41.176 68 39 1 43 110 105 171 9.46e-12 60.1
Msa0810610 MtrunA17_Chr7g0236341 45.205 73 38 1 33 105 500 570 1.01e-11 60.1
Msa0810610 MtrunA17_Chr4g0048581 51.724 58 26 1 48 105 528 583 1.08e-11 60.1
Msa0810610 MtrunA17_Chr3g0091461 46.269 67 34 1 37 103 789 853 1.20e-11 60.1
Msa0810610 MtrunA17_Chr7g0236571 47.059 68 34 1 38 105 326 391 1.24e-11 59.7
Msa0810610 MtrunA17_Chr6g0474861 41.892 74 37 2 41 110 272 343 1.27e-11 59.7
Msa0810610 MtrunA17_Chr6g0474831 43.243 74 36 2 41 110 13 84 1.55e-11 59.3
Msa0810610 MtrunA17_Chr4g0053521 34.951 103 65 1 3 105 1010 1110 1.59e-11 59.7
Msa0810610 MtrunA17_Chr2g0292501 35.238 105 62 2 5 105 470 572 1.65e-11 59.7
Msa0810610 MtrunA17_Chr5g0428571 35.052 97 61 1 14 110 261 355 1.74e-11 59.3
Msa0810610 MtrunA17_Chr2g0277731 44.444 81 38 3 29 105 278 355 1.83e-11 59.3
Msa0810610 MtrunA17_Chr1g0154311 46.377 69 31 2 41 105 337 403 1.85e-11 59.3
Msa0810610 MtrunA17_Chr2g0280771 41.667 72 40 1 39 110 470 539 2.08e-11 59.3
Msa0810610 MtrunA17_Chr1g0181291 45.902 61 33 0 45 105 505 565 2.64e-11 58.9
Msa0810610 MtrunA17_Chr7g0236551 35.238 105 60 3 7 105 438 540 2.72e-11 58.9
Msa0810610 MtrunA17_Chr2g0278861 51.786 56 26 1 48 103 285 339 2.74e-11 58.9
Msa0810610 MtrunA17_Chr8g0342071 51.613 62 28 1 45 106 443 502 2.84e-11 58.9
Msa0810610 MtrunA17_Chr8g0369211 45.588 68 35 1 45 112 622 687 2.90e-11 58.9
Msa0810610 MtrunA17_Chr8g0340081 35.354 99 62 1 7 105 462 558 2.95e-11 58.9
Msa0810610 MtrunA17_Chr1g0206451 40.260 77 44 1 34 110 355 429 3.36e-11 58.5
Msa0810610 MtrunA17_Chr8g0354281 43.662 71 38 1 40 110 324 392 3.55e-11 58.5
Msa0810610 MtrunA17_Chr5g0428581 43.939 66 35 1 40 105 33 96 3.55e-11 58.5
Msa0810610 MtrunA17_Chr2g0280731 37.778 90 54 1 16 105 515 602 3.59e-11 58.5
Msa0810610 MtrunA17_Chr6g0475091 41.892 74 37 2 41 110 567 638 3.75e-11 58.5
Msa0810610 MtrunA17_Chr6g0453571 42.029 69 38 1 42 110 331 397 4.16e-11 58.2
Msa0810610 MtrunA17_Chr2g0318301 38.679 106 58 4 4 105 451 553 4.23e-11 58.5
Msa0810610 MtrunA17_Chr3g0081061 41.791 67 37 1 39 105 25 89 4.27e-11 55.8
Msa0810610 MtrunA17_Chr6g0475201 40.541 74 38 2 41 110 560 631 4.29e-11 58.2
Msa0810610 MtrunA17_Chr4g0042341 43.750 64 34 1 42 105 514 575 4.31e-11 58.2
Msa0810610 MtrunA17_Chr2g0323231 40.909 66 37 1 45 110 172 235 4.34e-11 58.2
Msa0810610 MtrunA17_Chr8g0340111 50.820 61 28 1 45 105 498 556 5.03e-11 58.2
Msa0810610 MtrunA17_Chr4g0042361 41.791 67 37 1 39 105 527 591 5.25e-11 58.2
Msa0810610 MtrunA17_Chr2g0280751 47.541 61 30 1 45 105 520 578 5.50e-11 58.2
Msa0810610 MtrunA17_Chr3g0081031 41.791 67 37 1 39 105 340 404 5.83e-11 57.8
Msa0810610 MtrunA17_Chr8g0364671 36.364 77 47 1 30 106 41 115 7.17e-11 57.4
Msa0810610 MtrunA17_Chr7g0221191 48.387 62 30 1 45 106 388 447 7.66e-11 57.8
Msa0810610 MtrunA17_Chr5g0413981 33.333 78 51 1 30 106 41 118 7.70e-11 57.4
Msa0810610 MtrunA17_Chr1g0203631 43.939 66 35 1 47 112 988 1051 7.92e-11 57.8
Msa0810610 MtrunA17_Chr2g0280831 44.776 67 35 1 39 105 83 147 8.07e-11 57.4
Msa0810610 MtrunA17_Chr4g0048631 45.312 64 33 1 42 105 523 584 8.28e-11 57.4
Msa0810610 MtrunA17_Chr1g0206291 42.029 69 38 1 42 110 344 410 8.47e-11 57.4
Msa0810610 MtrunA17_Chr3g0112111 42.308 78 35 3 29 105 333 401 8.78e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0810610 AT2G01820.1 56.180 89 39 0 17 105 550 638 1.26e-25 100
Msa0810610 AT1G24650.1 48.454 97 50 0 9 105 504 600 4.11e-23 93.2
Msa0810610 AT1G66150.1 59.155 71 29 0 35 105 571 641 1.25e-20 85.9
Msa0810610 AT3G23750.1 53.623 69 32 0 37 105 563 631 6.74e-19 80.9
Msa0810610 AT5G28680.1 35.849 106 62 4 3 105 470 572 3.08e-14 67.8
Msa0810610 AT5G28680.2 35.849 106 62 4 3 105 470 572 3.08e-14 67.8
Msa0810610 AT1G61390.2 44.928 69 36 1 42 110 342 408 4.63e-13 64.3
Msa0810610 AT1G61390.1 44.928 69 36 1 42 110 510 576 4.76e-13 64.3
Msa0810610 AT3G46290.1 47.826 69 34 1 37 105 470 536 8.69e-13 63.5
Msa0810610 AT1G61430.4 39.130 92 48 2 20 105 428 517 2.39e-12 62.4
Msa0810610 AT1G61430.2 39.130 92 48 2 20 105 451 540 2.48e-12 62.4
Msa0810610 AT1G61430.6 39.130 92 48 2 20 105 451 540 2.48e-12 62.4
Msa0810610 AT1G61430.3 39.130 92 48 2 20 105 460 549 2.63e-12 62.0
Msa0810610 AT1G61430.8 38.710 93 49 2 20 106 451 541 3.29e-12 62.0
Msa0810610 AT1G61430.7 38.710 93 49 2 20 106 451 541 3.29e-12 62.0
Msa0810610 AT1G61430.9 38.710 93 49 2 20 106 451 541 3.38e-12 62.0
Msa0810610 AT1G61430.10 38.710 93 49 2 20 106 428 518 3.41e-12 62.0
Msa0810610 AT1G61430.11 38.710 93 49 2 20 106 428 518 3.54e-12 62.0
Msa0810610 AT1G61430.12 38.710 93 49 2 20 106 428 518 3.54e-12 62.0
Msa0810610 AT1G61430.13 38.710 93 49 2 20 106 428 518 3.54e-12 62.0
Msa0810610 AT1G61380.1 49.231 65 31 1 42 106 478 540 4.27e-12 61.6
Msa0810610 AT1G61380.2 49.231 65 31 1 42 106 331 393 4.85e-12 61.2
Msa0810610 AT5G59700.1 46.970 66 33 1 40 105 470 533 5.14e-12 61.2
Msa0810610 AT4G21410.2 40.541 74 42 1 37 110 342 413 5.86e-12 61.2
Msa0810610 AT1G61460.1 38.824 85 45 2 26 105 423 505 7.67e-12 60.8
Msa0810610 AT1G11300.2 49.254 67 32 1 39 105 481 545 8.93e-12 60.8
Msa0810610 AT1G79620.5 47.059 68 34 1 45 112 657 722 9.33e-12 60.5
Msa0810610 AT1G11300.1 49.254 67 32 1 39 105 496 560 9.39e-12 60.5
Msa0810610 AT1G79620.1 47.059 68 34 1 45 112 631 696 9.50e-12 60.5
Msa0810610 AT5G54380.1 50.000 64 30 1 42 105 500 561 9.98e-12 60.5
Msa0810610 AT1G61420.3 43.478 69 37 1 42 110 334 400 1.09e-11 60.5
Msa0810610 AT1G61420.5 43.478 69 37 1 42 110 336 402 1.09e-11 60.5
Msa0810610 AT1G61400.2 47.692 65 32 1 42 106 490 552 1.12e-11 60.5
Msa0810610 AT1G79620.3 47.059 68 34 1 45 112 631 696 1.13e-11 60.5
Msa0810610 AT1G11050.1 42.857 77 41 2 30 105 272 346 1.15e-11 60.5
Msa0810610 AT1G61400.1 47.692 65 32 1 42 106 490 552 1.15e-11 60.5
Msa0810610 AT1G79620.2 47.059 68 34 1 45 112 631 696 1.18e-11 60.5
Msa0810610 AT4G27300.1 43.056 72 36 2 37 105 482 551 1.32e-11 60.1
Msa0810610 AT4G21410.3 43.478 69 37 1 37 105 342 408 1.70e-11 59.7
Msa0810610 AT1G11303.1 44.156 77 41 1 29 105 486 560 1.82e-11 59.7
Msa0810610 AT4G21380.2 48.438 64 29 2 48 111 522 581 1.91e-11 59.7
Msa0810610 AT1G61440.3 37.634 93 50 2 20 106 445 535 1.92e-11 59.7
Msa0810610 AT4G21410.1 42.857 70 38 1 37 106 217 284 1.97e-11 59.7
Msa0810610 AT1G68690.4 48.387 62 30 1 45 106 370 429 2.04e-11 59.7
Msa0810610 AT1G61360.1 48.438 64 31 1 42 105 488 549 2.06e-11 59.7
Msa0810610 AT3G09780.1 44.118 68 37 1 45 111 513 580 2.13e-11 59.7
Msa0810610 AT4G23160.3 37.647 85 51 1 26 110 913 995 2.26e-11 59.7
Msa0810610 AT4G23160.2 37.647 85 51 1 26 110 913 995 2.26e-11 59.7
Msa0810610 AT1G61360.2 48.438 64 31 1 42 105 407 468 2.28e-11 59.7
Msa0810610 AT1G61360.3 48.438 64 31 1 42 105 407 468 2.28e-11 59.7
Msa0810610 AT1G61360.4 48.438 64 31 1 42 105 407 468 2.28e-11 59.7
Msa0810610 AT1G61440.1 35.644 101 63 1 6 106 432 530 2.40e-11 59.3
Msa0810610 AT4G23160.1 37.647 85 51 1 26 110 333 415 2.42e-11 59.3
Msa0810610 AT1G68690.1 42.254 71 39 1 36 106 361 429 2.45e-11 59.3
Msa0810610 AT1G68690.3 42.254 71 39 1 36 106 361 429 2.45e-11 59.3
Msa0810610 AT1G68690.2 42.254 71 39 1 36 106 361 429 2.45e-11 59.3
Msa0810610 AT1G65800.2 46.377 69 33 2 43 111 514 578 2.66e-11 59.3
Msa0810610 AT4G23140.1 37.647 85 51 1 26 110 325 407 2.66e-11 59.3
Msa0810610 AT4G21400.3 38.158 76 45 1 35 110 332 405 2.71e-11 59.3
Msa0810610 AT4G23140.2 37.647 85 51 1 26 110 325 407 2.72e-11 59.3
Msa0810610 AT4G21400.6 38.158 76 45 1 35 110 344 417 2.74e-11 59.3
Msa0810610 AT4G21400.4 38.158 76 45 1 35 110 344 417 2.74e-11 59.3
Msa0810610 AT4G21400.5 38.158 76 45 1 35 110 344 417 2.74e-11 59.3
Msa0810610 AT4G21400.8 38.158 76 45 1 35 110 205 278 2.75e-11 59.3
Msa0810610 AT4G21400.2 38.158 76 45 1 35 110 332 405 2.79e-11 59.3
Msa0810610 AT4G21400.7 38.158 76 45 1 35 110 306 379 2.79e-11 59.3
Msa0810610 AT4G21400.1 38.158 76 45 1 35 110 344 417 2.80e-11 59.3
Msa0810610 AT3G58690.1 44.776 67 35 1 39 105 74 138 3.04e-11 58.9
Msa0810610 AT1G65790.3 48.438 64 29 2 48 111 515 574 3.23e-11 58.9
Msa0810610 AT1G65790.2 48.438 64 29 2 48 111 515 574 3.23e-11 58.9
Msa0810610 AT1G70740.1 40.000 80 44 2 33 110 41 118 3.39e-11 58.9
Msa0810610 AT5G38560.1 46.774 62 31 1 45 106 332 391 3.71e-11 58.9
Msa0810610 AT4G21390.1 43.284 67 36 1 39 105 516 580 3.77e-11 58.9
Msa0810610 AT1G70740.2 40.000 80 44 2 33 110 29 106 3.78e-11 58.9
Msa0810610 AT1G61500.3 46.154 65 33 1 42 106 486 548 3.80e-11 58.9
Msa0810610 AT3G04690.1 34.579 107 62 5 3 105 466 568 3.92e-11 58.9
Msa0810610 AT1G61500.1 46.154 65 33 1 42 106 481 543 3.99e-11 58.9
Msa0810610 AT4G21390.2 43.284 67 36 1 39 105 516 580 4.01e-11 58.9
Msa0810610 AT4G33430.1 46.774 62 32 1 42 103 279 339 4.12e-11 58.9
Msa0810610 AT1G61500.2 46.154 65 33 1 42 106 463 525 4.22e-11 58.9
Msa0810610 AT1G61420.2 46.875 64 32 1 42 105 486 547 4.80e-11 58.5
Msa0810610 AT1G61420.4 46.875 64 32 1 42 105 486 547 4.80e-11 58.5
Msa0810610 AT1G11280.3 42.029 69 38 1 42 110 483 549 4.94e-11 58.5
Msa0810610 AT1G11280.2 42.029 69 38 1 42 110 495 561 4.94e-11 58.5
Msa0810610 AT1G11280.1 42.029 69 38 1 42 110 505 571 4.95e-11 58.5
Msa0810610 AT5G61350.1 43.939 66 35 1 40 105 513 576 4.95e-11 58.5
Msa0810610 AT4G39110.1 49.180 61 29 1 45 105 519 577 4.97e-11 58.5
Msa0810610 AT1G11280.4 42.029 69 38 1 42 110 493 559 4.99e-11 58.5
Msa0810610 AT1G61420.1 46.875 64 32 1 42 105 484 545 5.03e-11 58.5
Msa0810610 AT1G11280.5 42.029 69 38 1 42 110 495 561 5.46e-11 58.5
Msa0810610 AT4G00970.2 36.000 100 62 1 6 105 298 395 6.09e-11 58.2
Msa0810610 AT4G00970.1 36.000 100 62 1 6 105 298 395 6.89e-11 58.2
Msa0810610 AT1G19390.1 48.387 62 30 1 45 106 444 503 6.99e-11 58.2
Msa0810610 AT5G38990.1 47.692 65 31 2 42 105 515 577 8.00e-11 58.2
Msa0810610 AT4G21380.1 48.438 64 29 2 48 111 522 581 8.79e-11 57.8
Msa0810610 AT1G16670.1 36.264 91 45 2 31 110 9 97 9.79e-11 57.8
Msa0810610 AT1G70450.1 45.161 62 32 1 45 106 42 101 9.92e-11 57.8

Find 24 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCTGGGGAGAGGCGATTT+TGG 0.227893 5_3:+39201940 Msa0810610:CDS
GCTTGTCTCAAAACTTGAAT+TGG 0.299756 5_3:-39201892 None:intergenic
GAAGAATGGTTGTTGTAAAA+AGG 0.361623 5_3:+39201992 Msa0810610:CDS
AATCAAACATCAACCATTGA+TGG 0.364988 5_3:-39202379 None:intergenic
AATGGTATACAAAGGAGAAT+TGG 0.416857 5_3:+39201963 Msa0810610:CDS
GCTTTCACTAAAGTTGTTTG+TGG 0.428019 5_3:-39201914 None:intergenic
TGAAATGGAGGTTCTTAGTA+AGG 0.443014 5_3:+39202062 Msa0810610:CDS
CAATGAGTTAAAGTCTGAAA+TGG 0.444144 5_3:+39202047 Msa0810610:CDS
CTGAAATGGTTAGTGACAAC+GGG 0.470216 5_3:+39202022 Msa0810610:CDS
TTCCAAAATCGCCTCTCCCC+AGG 0.495145 5_3:-39201942 None:intergenic
CTTTAGTGAAAGCAACATCC+TGG 0.497024 5_3:+39201924 Msa0810610:CDS
CTCAATTGTCAATCCATCAA+TGG 0.503650 5_3:+39202366 Msa0810610:CDS
AGAATTGGACGATGGAAGAA+TGG 0.508160 5_3:+39201978 Msa0810610:CDS
CCTGCAGTATGGTTGCATCA+TGG 0.528181 5_3:+39201764 None:intergenic
TTTAGTGAAAGCAACATCCT+GGG 0.545525 5_3:+39201925 Msa0810610:CDS
ACTATGTGCAACTGTTGCAC+TGG 0.547330 5_3:-39201818 None:intergenic
GAAAGCAACATCCTGGGGAG+AGG 0.561671 5_3:+39201931 Msa0810610:CDS
CATAGTGGTCATTCTAGAGA+TGG 0.571005 5_3:+39201835 Msa0810610:CDS
TGAGTTAAAGTCTGAAATGG+AGG 0.617703 5_3:+39202050 Msa0810610:CDS
AGTGCAACAGTTGCACATAG+TGG 0.620945 5_3:+39201820 Msa0810610:CDS
GCTGAAATGGTTAGTGACAA+CGG 0.646083 5_3:+39202021 Msa0810610:CDS
TACAAAGGAGAATTGGACGA+TGG 0.659170 5_3:+39201970 Msa0810610:CDS
TTAGTAGCAATTCGTCTGTG+TGG 0.666585 5_3:-39201859 None:intergenic
TTAGTGAAAGCAACATCCTG+GGG 0.694507 5_3:+39201926 Msa0810610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGCTTATAAATATTTTA+TGG - chr5_3:39202306-39202325 None:intergenic 10.0%
!!! TATTTAATAGTTGCTTGTTT+TGG + chr5_3:39202241-39202260 Msa0810610:intron 20.0%
!!! ATATTAGTTTTTCGTCTGTG+TGG + chr5_3:39201787-39201806 Msa0810610:CDS 30.0%
!!! GATTTTGGAATGGTATACAA+AGG + chr5_3:39201955-39201974 Msa0810610:CDS 30.0%
AATGGTATACAAAGGAGAAT+TGG + chr5_3:39201963-39201982 Msa0810610:CDS 30.0%
GAAGAATGGTTGTTGTAAAA+AGG + chr5_3:39201992-39202011 Msa0810610:CDS 30.0%
AATGGTTGTTGTAAAAAGGA+TGG + chr5_3:39201996-39202015 Msa0810610:CDS 30.0%
CAATGAGTTAAAGTCTGAAA+TGG + chr5_3:39202047-39202066 Msa0810610:CDS 30.0%
ATTACCTGTATAATAGCCAA+TGG + chr5_3:39202340-39202359 Msa0810610:intron 30.0%
GCTTGTCTCAAAACTTGAAT+TGG - chr5_3:39201895-39201914 None:intergenic 35.0%
GCTTTCACTAAAGTTGTTTG+TGG - chr5_3:39201917-39201936 None:intergenic 35.0%
TTTAGTGAAAGCAACATCCT+GGG + chr5_3:39201925-39201944 Msa0810610:CDS 35.0%
! TGAGTTAAAGTCTGAAATGG+AGG + chr5_3:39202050-39202069 Msa0810610:CDS 35.0%
TGAAATGGAGGTTCTTAGTA+AGG + chr5_3:39202062-39202081 Msa0810610:CDS 35.0%
TAAGGTTAGACACAAGCATT+TGG + chr5_3:39202080-39202099 Msa0810610:intron 35.0%
CTCAATTGTCAATCCATCAA+TGG + chr5_3:39202366-39202385 Msa0810610:CDS 35.0%
! CATAGTGGTCATTCTAGAGA+TGG + chr5_3:39201835-39201854 Msa0810610:CDS 40.0%
TTAGTAGCAATTCGTCTGTG+TGG - chr5_3:39201862-39201881 None:intergenic 40.0%
CTTTAGTGAAAGCAACATCC+TGG + chr5_3:39201924-39201943 Msa0810610:CDS 40.0%
TTAGTGAAAGCAACATCCTG+GGG + chr5_3:39201926-39201945 Msa0810610:CDS 40.0%
TACAAAGGAGAATTGGACGA+TGG + chr5_3:39201970-39201989 Msa0810610:CDS 40.0%
AGAATTGGACGATGGAAGAA+TGG + chr5_3:39201978-39201997 Msa0810610:CDS 40.0%
AAAAAGGATGGCAGCTGAAA+TGG + chr5_3:39202008-39202027 Msa0810610:CDS 40.0%
GCTGAAATGGTTAGTGACAA+CGG + chr5_3:39202021-39202040 Msa0810610:CDS 40.0%
CTGAAATGGTTAGTGACAAC+GGG + chr5_3:39202022-39202041 Msa0810610:CDS 40.0%
TATTGTCCTTACCTGTCTCA+AGG + chr5_3:39202143-39202162 Msa0810610:intron 40.0%
ATTGACAATTGAGCTGCCAT+TGG - chr5_3:39202359-39202378 None:intergenic 40.0%
ACTATGTGCAACTGTTGCAC+TGG - chr5_3:39201821-39201840 None:intergenic 45.0%
AGTGCAACAGTTGCACATAG+TGG + chr5_3:39201820-39201839 Msa0810610:CDS 45.0%
AAGCATTTGGTTGCACTTCG+TGG + chr5_3:39202093-39202112 Msa0810610:intron 45.0%
TCTCATTGTCGTCCAAGCAA+TGG - chr5_3:39202119-39202138 None:intergenic 45.0%
GATGCTTAACCAATGCCCTT+GGG + chr5_3:39202263-39202282 Msa0810610:intron 45.0%
ATGCTTAACCAATGCCCTTG+GGG + chr5_3:39202264-39202283 Msa0810610:intron 45.0%
AATGCTAACCAATGCCCCAA+GGG - chr5_3:39202281-39202300 None:intergenic 45.0%
! GCTGCCATTGGCTATTATAC+AGG - chr5_3:39202347-39202366 None:intergenic 45.0%
! AGAGTGCCTTGAGACAGGTA+AGG - chr5_3:39202152-39202171 None:intergenic 50.0%
!! TGCTAAGAGTGCCTTGAGAC+AGG - chr5_3:39202157-39202176 None:intergenic 50.0%
GGATGCTTAACCAATGCCCT+TGG + chr5_3:39202262-39202281 Msa0810610:intron 50.0%
GAATGCTAACCAATGCCCCA+AGG - chr5_3:39202282-39202301 None:intergenic 50.0%
GAAAGCAACATCCTGGGGAG+AGG + chr5_3:39201931-39201950 Msa0810610:CDS 55.0%
! ATCCTGGGGAGAGGCGATTT+TGG + chr5_3:39201940-39201959 Msa0810610:CDS 55.0%
TTCCAAAATCGCCTCTCCCC+AGG - chr5_3:39201945-39201964 None:intergenic 55.0%
!! GGGGAGAGGCGATTTTGGAA+TGG + chr5_3:39201945-39201964 Msa0810610:CDS 55.0%
TGCACTTCGTGGCCATTGCT+TGG + chr5_3:39202104-39202123 Msa0810610:intron 55.0%
AACCAATGCCCTTGGGGCAT+TGG + chr5_3:39202270-39202289 Msa0810610:intron 55.0%
! AACCAATGCCCCAAGGGCAT+TGG - chr5_3:39202275-39202294 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 39201772 39202392 39201772 ID=Msa0810610;Name=Msa0810610
chr5_3 mRNA 39201772 39202392 39201772 ID=Msa0810610-mRNA-1;Parent=Msa0810610;Name=Msa0810610-mRNA-1;_AED=0.61;_eAED=0.61;_QI=0|0|0|0.5|1|1|2|0|112
chr5_3 exon 39201772 39202083 39201772 ID=Msa0810610-mRNA-1:exon:11905;Parent=Msa0810610-mRNA-1
chr5_3 exon 39202366 39202392 39202366 ID=Msa0810610-mRNA-1:exon:11906;Parent=Msa0810610-mRNA-1
chr5_3 CDS 39201772 39202083 39201772 ID=Msa0810610-mRNA-1:cds;Parent=Msa0810610-mRNA-1
chr5_3 CDS 39202366 39202392 39202366 ID=Msa0810610-mRNA-1:cds;Parent=Msa0810610-mRNA-1
Gene Sequence

>Msa0810610

ATGGTTGCATCATGGATATTAGTTTTTCGTCTGTGTGGTCACACTTCCAGTGCAACAGTTGCACATAGTGGTCATTCTAGAGATGGACCACACAGACGAATTGCTACTAATATGATCATTCCAATTCAAGTTTTGAGACAAGCCACAAACAACTTTAGTGAAAGCAACATCCTGGGGAGAGGCGATTTTGGAATGGTATACAAAGGAGAATTGGACGATGGAAGAATGGTTGTTGTAAAAAGGATGGCAGCTGAAATGGTTAGTGACAACGGGTTCAATGAGTTAAAGTCTGAAATGGAGGTTCTTAGTAAGCTCAATTGTCAATCCATCAATGGTTGA
Protein sequence

>Msa0810610

MVASWILVFRLCGHTSSATVAHSGHSRDGPHRRIATNMIIPIQVLRQATNNFSESNILGRGDFGMVYKGELDDGRMVVVKRMAAEMVSDNGFNELKSEMEVLSKLNCQSING*