AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0814700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0814700 MtrunA17_Chr5g0422571 93.160 307 9 3 1 307 1 295 0.0 587
Msa0814700 MtrunA17_Chr1g0198651 70.120 251 60 5 21 269 23 260 9.72e-116 335
Msa0814700 MtrunA17_Chr2g0328921 67.890 218 56 4 68 274 84 298 1.32e-106 313
Msa0814700 MtrunA17_Chr1g0173841 61.538 247 60 4 71 288 144 384 5.80e-106 314
Msa0814700 MtrunA17_Chr7g0258011 62.821 234 60 3 76 288 118 345 1.31e-103 306
Msa0814700 MtrunA17_Chr8g0352011 62.174 230 64 5 76 288 112 335 1.23e-102 304
Msa0814700 MtrunA17_Chr2g0321291 62.009 229 68 5 63 274 68 294 3.60e-100 296
Msa0814700 MtrunA17_Chr4g0026521 63.470 219 64 4 78 283 76 291 2.30e-98 291
Msa0814700 MtrunA17_Chr4g0053341 64.390 205 63 3 68 269 32 229 1.25e-94 279
Msa0814700 MtrunA17_Chr1g0200421 38.596 171 87 8 108 264 226 392 1.22e-24 103
Msa0814700 MtrunA17_Chr1g0181381 33.163 196 113 5 77 263 10 196 1.86e-23 100
Msa0814700 MtrunA17_Chr8g0382121 40.278 144 80 5 128 267 127 268 2.14e-23 97.8
Msa0814700 MtrunA17_Chr4g0029521 33.000 200 123 6 64 261 4 194 5.03e-23 99.0
Msa0814700 MtrunA17_Chr4g0040571 31.771 192 120 4 85 275 27 208 2.42e-22 97.1
Msa0814700 MtrunA17_Chr2g0320341 38.065 155 88 5 116 264 235 387 3.69e-22 95.5
Msa0814700 MtrunA17_Chr1g0151351 35.057 174 101 6 89 260 33 196 6.79e-22 95.5
Msa0814700 MtrunA17_Chr1g0199101 36.306 157 87 6 113 267 63 208 1.11e-21 95.1
Msa0814700 MtrunA17_Chr7g0263941 33.889 180 106 6 86 263 23 191 1.83e-21 93.6
Msa0814700 MtrunA17_Chr1g0185121 30.841 214 135 6 66 276 8 211 2.23e-21 94.0
Msa0814700 MtrunA17_Chr8g0373541 33.005 203 112 7 93 281 26 218 2.28e-21 94.0
Msa0814700 MtrunA17_Chr8g0342421 34.868 152 86 5 120 269 85 225 2.66e-21 94.0
Msa0814700 MtrunA17_Chr2g0330171 35.443 158 88 6 108 263 58 203 4.47e-21 93.2
Msa0814700 MtrunA17_Chr7g0268961 37.500 144 77 6 120 261 45 177 4.61e-21 93.2
Msa0814700 MtrunA17_Chr2g0326381 38.571 140 76 4 120 258 55 185 1.05e-20 91.7
Msa0814700 MtrunA17_Chr1g0210821 35.374 147 84 4 118 263 65 201 1.37e-20 92.0
Msa0814700 MtrunA17_Chr1g0191991 35.443 158 83 6 114 263 74 220 2.62e-20 90.9
Msa0814700 MtrunA17_Chr1g0162971 37.671 146 78 5 120 263 64 198 2.90e-20 90.5
Msa0814700 MtrunA17_Chr1g0163001 35.294 153 86 5 113 263 59 200 8.18e-20 89.7
Msa0814700 MtrunA17_Chr3g0123941 39.583 144 78 7 128 267 139 277 3.55e-19 86.3
Msa0814700 MtrunA17_Chr3g0141431 33.721 172 102 6 92 261 17 178 3.58e-19 87.0
Msa0814700 MtrunA17_Chr2g0324991 36.806 144 78 7 113 253 8 141 1.66e-17 78.2
Msa0814700 MtrunA17_Chr6g0479921 30.612 147 89 6 118 261 50 186 4.46e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0814700 AT1G13290.1 67.681 263 57 6 21 278 12 251 1.87e-117 340
Msa0814700 AT3G57670.1 72.632 190 40 2 108 297 199 376 7.92e-101 301
Msa0814700 AT1G51220.1 53.952 291 102 6 7 269 34 320 3.57e-100 298
Msa0814700 AT3G20880.1 63.594 217 46 4 54 264 204 393 2.20e-97 293
Msa0814700 AT1G08290.1 70.056 177 45 1 110 286 164 332 1.15e-95 286
Msa0814700 AT1G34790.1 63.333 210 64 3 76 273 83 291 2.26e-91 274
Msa0814700 AT1G34370.3 38.235 170 91 6 108 264 9 177 6.21e-27 107
Msa0814700 AT1G34370.2 40.397 151 83 5 120 264 238 387 2.22e-26 108
Msa0814700 AT1G34370.1 40.397 151 83 5 120 264 238 387 2.22e-26 108
Msa0814700 AT5G22890.1 39.785 186 94 11 91 264 181 360 1.67e-24 102
Msa0814700 AT5G66730.1 32.673 202 121 6 71 268 4 194 1.18e-22 98.2
Msa0814700 AT1G55110.3 37.013 154 85 5 118 269 84 227 5.35e-22 95.9
Msa0814700 AT1G55110.1 37.013 154 85 5 118 269 84 227 5.35e-22 95.9
Msa0814700 AT1G55110.2 37.013 154 85 5 118 269 84 227 5.35e-22 95.9
Msa0814700 AT3G50700.1 32.673 202 123 5 71 269 6 197 1.07e-21 95.1
Msa0814700 AT5G03150.1 37.063 143 80 4 120 261 76 209 1.51e-21 95.1
Msa0814700 AT5G44160.1 33.523 176 106 4 94 268 34 199 1.66e-21 94.7
Msa0814700 AT2G02080.1 37.255 153 83 5 118 268 75 216 1.87e-21 94.7
Msa0814700 AT2G02080.4 37.255 153 83 5 118 268 75 216 1.87e-21 94.7
Msa0814700 AT2G02080.5 37.255 153 83 5 118 268 75 216 1.87e-21 94.7
Msa0814700 AT2G02080.3 37.255 153 83 5 118 268 75 216 1.87e-21 94.7
Msa0814700 AT2G02070.2 37.255 153 83 5 118 268 73 214 2.27e-21 94.7
Msa0814700 AT2G02070.1 37.255 153 83 5 118 268 73 214 2.27e-21 94.7
Msa0814700 AT1G03840.1 33.523 176 106 5 94 268 38 203 2.96e-21 94.4
Msa0814700 AT1G03840.2 34.091 176 103 7 94 268 38 201 5.21e-21 93.6
Msa0814700 AT5G44160.2 36.000 150 85 4 120 268 21 160 5.61e-21 92.8
Msa0814700 AT2G02080.6 37.086 151 82 5 121 269 1 140 8.37e-21 92.4
Msa0814700 AT2G02080.2 37.086 151 82 5 121 269 1 140 8.37e-21 92.4
Msa0814700 AT1G14580.1 36.986 146 79 5 118 261 74 208 3.94e-20 90.9
Msa0814700 AT1G14580.2 36.986 146 79 5 118 261 74 208 3.94e-20 90.9
Msa0814700 AT1G14580.3 36.986 146 79 5 118 261 88 222 5.03e-20 90.5
Msa0814700 AT3G45260.2 35.333 150 87 4 120 268 62 202 6.19e-20 90.1
Msa0814700 AT3G45260.1 35.333 150 87 4 120 268 62 202 6.19e-20 90.1
Msa0814700 AT5G60470.2 34.104 173 104 5 92 263 39 202 6.44e-20 90.1
Msa0814700 AT5G60470.3 34.104 173 104 5 92 263 35 198 8.11e-20 89.7
Msa0814700 AT3G13810.2 34.615 156 92 4 113 267 87 233 9.13e-20 89.7
Msa0814700 AT5G60470.1 37.241 145 81 5 120 263 58 193 9.33e-20 89.4
Msa0814700 AT3G13810.4 34.615 156 92 4 113 267 86 232 9.64e-20 89.7
Msa0814700 AT3G13810.1 34.615 156 92 4 113 267 86 232 9.64e-20 89.7
Msa0814700 AT3G13810.3 34.615 156 92 4 113 267 73 219 1.00e-19 89.7
Msa0814700 AT3G13810.5 34.615 156 92 4 113 267 81 227 1.12e-19 89.7
Msa0814700 AT3G13810.6 35.099 151 88 4 118 267 72 213 1.22e-19 89.4
Msa0814700 AT4G02670.2 34.211 152 90 4 113 263 71 213 8.52e-19 86.3
Msa0814700 AT4G02670.1 34.211 152 90 4 113 263 69 211 9.63e-19 86.3
Msa0814700 AT1G25250.3 30.216 139 88 4 118 254 54 185 2.12e-11 64.3
Msa0814700 AT1G25250.1 30.216 139 88 4 118 254 31 162 2.13e-11 64.3

Find 67 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACTCCCCTTAGAGATTC+TGG 0.179929 5_3:-46959869 None:intergenic
CTCCTATTCAAGGTCAATAT+TGG 0.253994 5_3:+46959111 Msa0814700:CDS
TCAACAGTGTCATTACTATT+AGG 0.287208 5_3:-46958837 None:intergenic
AAAGATCATGTTCGTGCTTT+CGG 0.314009 5_3:+46960157 Msa0814700:CDS
GGTTTGTGGTTGTGTGTTCC+AGG 0.317646 5_3:-46958891 None:intergenic
GAAAAGGACCAGAATCTCTA+AGG 0.322420 5_3:+46959861 Msa0814700:CDS
TGATGATCAGGTTTACTAAT+AGG 0.342578 5_3:-46959077 None:intergenic
TCACTCTCACTTGGCCCTCC+TGG 0.348650 5_3:+46958873 Msa0814700:CDS
TTTAATGTGGAGGATAGTTT+AGG 0.370427 5_3:-46958912 None:intergenic
TAGTAAACCTGATCATCATT+TGG 0.397302 5_3:+46959082 Msa0814700:CDS
AGCATGGAGCGAAGCCATTC+GGG 0.401651 5_3:+46960017 Msa0814700:CDS
TGGAGGATAGTTTAGGTTTG+TGG 0.408461 5_3:-46958905 None:intergenic
GGGAAACCATTTGCAGTTAG+AGG 0.410419 5_3:+46960052 Msa0814700:CDS
GAAGATCAGGAAGTATTGTT+AGG 0.411354 5_3:+46960214 Msa0814700:CDS
TTGGCTTCAGCTCCTATTCA+AGG 0.424273 5_3:+46959101 Msa0814700:CDS
AAGCATGGAGCGAAGCCATT+CGG 0.440022 5_3:+46960016 Msa0814700:CDS
CATGTTCGTGCTTTCGGTGA+TGG 0.445219 5_3:+46960163 Msa0814700:CDS
TCTCCTGCTCAAATCCTCAT+TGG 0.454551 5_3:+46959140 Msa0814700:CDS
CGTTGAGAGTTGTGAAGATC+AGG 0.455624 5_3:+46960201 Msa0814700:CDS
ACCACTTTGCTCATCATCAA+TGG 0.458380 5_3:-46959000 None:intergenic
CAACCGTTTCAATAATATGC+AGG 0.466033 5_3:+46959202 Msa0814700:CDS
CAGTCACCTCTAACTGCAAA+TGG 0.466119 5_3:-46960058 None:intergenic
AGTAGAAGAGTGATTTAATG+TGG 0.470478 5_3:-46958925 None:intergenic
TCTTCCATTTCACTCTCACT+TGG 0.472544 5_3:+46958864 Msa0814700:CDS
GCTGAAGCCAAATGATGATC+AGG 0.472827 5_3:-46959089 None:intergenic
ATCCAATATTGACCTTGAAT+AGG 0.474167 5_3:-46959113 None:intergenic
TAACCTGCATATTATTGAAA+CGG 0.475929 5_3:-46959205 None:intergenic
TCCTTGAGTGGCCTTGACCT+TGG 0.476401 5_3:-46959962 None:intergenic
AGGTTCAATTGAGGATATGT+AGG 0.476900 5_3:-46958817 None:intergenic
AGTCTTAACATTGATGAAGC+TGG 0.481814 5_3:-46959893 None:intergenic
AGGGCCAAGTGAGAGTGAAA+TGG 0.483147 5_3:-46958868 None:intergenic
GTATATATCAGATGCATATG+TGG 0.492007 5_3:+46959819 Msa0814700:intron
ATTACTATTAGGTTCAATTG+AGG 0.492152 5_3:-46958826 None:intergenic
CCTTGCTATTGCTGTGCAGA+GGG 0.509164 5_3:+46959917 Msa0814700:CDS
TATATATCAGATGCATATGT+GGG 0.512415 5_3:+46959820 Msa0814700:intron
TCCTTGCTATTGCTGTGCAG+AGG 0.519467 5_3:+46959916 Msa0814700:CDS
CAGATGCATATGTGGGGACA+TGG 0.520986 5_3:+46959827 Msa0814700:intron
CATGGATCACAATACAGAAA+AGG 0.532079 5_3:+46959845 Msa0814700:CDS
CACTTCAAACACACTACAAG+AGG 0.550716 5_3:+46959993 Msa0814700:CDS
TGTGGTTGTGTGTTCCAGGA+GGG 0.551458 5_3:-46958887 None:intergenic
AAAAGGACCAGAATCTCTAA+GGG 0.555739 5_3:+46959862 Msa0814700:CDS
TCCAAGGTCAAGGCCACTCA+AGG 0.567553 5_3:+46959961 Msa0814700:CDS
CATTTGCAGTTAGAGGTGAC+TGG 0.568578 5_3:+46960059 Msa0814700:CDS
ATGAGGATTTGAGCAGGAGA+AGG 0.568596 5_3:-46959137 None:intergenic
TCACAACTCTCAACGGTGTG+TGG 0.569324 5_3:-46960193 None:intergenic
CTGATCTTCACAACTCTCAA+CGG 0.574461 5_3:-46960200 None:intergenic
GGTCCAATGAGGATTTGAGC+AGG 0.576870 5_3:-46959143 None:intergenic
GGAGAATGTGTGAGCTTGCA+TGG 0.580463 5_3:-46958796 None:intergenic
CCCTCTGCACAGCAATAGCA+AGG 0.583701 5_3:-46959917 None:intergenic
TAATGATAACATCGATCGAA+TGG 0.586962 5_3:+46960291 Msa0814700:CDS
CCATTCGGGTGTAGAAAGTG+TGG 0.590964 5_3:+46960031 Msa0814700:CDS
ACACACTACAAGAGGAAGCA+TGG 0.591744 5_3:+46960001 Msa0814700:CDS
AGAACAACATAGAGCATCCA+AGG 0.593445 5_3:+46959945 Msa0814700:CDS
CCACACTTTCTACACCCGAA+TGG 0.593700 5_3:-46960031 None:intergenic
GAATATGATGAGGAATTTGA+AGG 0.593705 5_3:+46960241 Msa0814700:CDS
ACAGTGCAAGAGAACTGTGT+TGG 0.625514 5_3:-46959164 None:intergenic
AGAAGAGTGATTTAATGTGG+AGG 0.629008 5_3:-46958922 None:intergenic
ACATAGAGCATCCAAGGTCA+AGG 0.630952 5_3:+46959951 Msa0814700:CDS
TTGTGGTTGTGTGTTCCAGG+AGG 0.639378 5_3:-46958888 None:intergenic
AGAACTGTGTTGGTCCAATG+AGG 0.645090 5_3:-46959154 None:intergenic
AGTGTTCTGAAATCCTTGAG+TGG 0.647415 5_3:-46959974 None:intergenic
GTAACAGTTGCACTTCACAT+TGG 0.647512 5_3:+46959023 Msa0814700:CDS
CATTCGGGTGTAGAAAGTGT+GGG 0.658306 5_3:+46960032 Msa0814700:CDS
ACCATTGATGATGAGCAAAG+TGG 0.669448 5_3:+46958999 Msa0814700:CDS
GTTAGGTGATGAATATGATG+AGG 0.719613 5_3:+46960231 Msa0814700:CDS
ATATATCAGATGCATATGTG+GGG 0.720271 5_3:+46959821 Msa0814700:intron
AAAGGACCAGAATCTCTAAG+GGG 0.721082 5_3:+46959863 Msa0814700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATAAACAAATATTAG+TGG + chr5_3:46959496-46959515 Msa0814700:intron 10.0%
!!! TTTTTATAAACAAATATTAG+TGG + chr5_3:46959496-46959515 Msa0814700:intron 10.0%
!! AAAAAAAAAAAGTTTACTTG+AGG - chr5_3:46959359-46959378 None:intergenic 15.0%
!! AAAAAAAAAAAGTTTACTTG+AGG - chr5_3:46959359-46959378 None:intergenic 15.0%
!!! ATTGTTCATTTTTATCTTCT+AGG - chr5_3:46959657-46959676 None:intergenic 20.0%
!!! ATTGTTCATTTTTATCTTCT+AGG - chr5_3:46959657-46959676 None:intergenic 20.0%
! ATTACTATTAGGTTCAATTG+AGG - chr5_3:46958829-46958848 None:intergenic 25.0%
! TAACCTGCATATTATTGAAA+CGG - chr5_3:46959208-46959227 None:intergenic 25.0%
!!! CTAGTTCTTGACAAATTTTA+TGG - chr5_3:46959537-46959556 None:intergenic 25.0%
!! AAATGACAAAATTTTGTGCT+TGG - chr5_3:46959752-46959771 None:intergenic 25.0%
! TATATATCAGATGCATATGT+GGG + chr5_3:46959820-46959839 Msa0814700:intron 25.0%
! ATTACTATTAGGTTCAATTG+AGG - chr5_3:46958829-46958848 None:intergenic 25.0%
! TAACCTGCATATTATTGAAA+CGG - chr5_3:46959208-46959227 None:intergenic 25.0%
!!! CTAGTTCTTGACAAATTTTA+TGG - chr5_3:46959537-46959556 None:intergenic 25.0%
!! AAATGACAAAATTTTGTGCT+TGG - chr5_3:46959752-46959771 None:intergenic 25.0%
! TATATATCAGATGCATATGT+GGG + chr5_3:46959820-46959839 Msa0814700:intron 25.0%
! TCAACAGTGTCATTACTATT+AGG - chr5_3:46958840-46958859 None:intergenic 30.0%
TTTAATGTGGAGGATAGTTT+AGG - chr5_3:46958915-46958934 None:intergenic 30.0%
AGTAGAAGAGTGATTTAATG+TGG - chr5_3:46958928-46958947 None:intergenic 30.0%
! CAATGGTAAAGTTTTGCTTT+TGG - chr5_3:46958986-46959005 None:intergenic 30.0%
! TGTTGTGTTTTGTGAAATGT+TGG - chr5_3:46959057-46959076 None:intergenic 30.0%
TGATGATCAGGTTTACTAAT+AGG - chr5_3:46959080-46959099 None:intergenic 30.0%
TAGTAAACCTGATCATCATT+TGG + chr5_3:46959082-46959101 Msa0814700:CDS 30.0%
ATCCAATATTGACCTTGAAT+AGG - chr5_3:46959116-46959135 None:intergenic 30.0%
AACGATTCAAGATGGTATTA+AGG - chr5_3:46959466-46959485 None:intergenic 30.0%
! AAAGTGTTAACGATTCAAGA+TGG - chr5_3:46959474-46959493 None:intergenic 30.0%
!!! AGGAGTATTGACAAATTTTC+TGG - chr5_3:46959574-46959593 None:intergenic 30.0%
ATGTCCTAATGCATACTATA+TGG + chr5_3:46959597-46959616 Msa0814700:intron 30.0%
TCTTCCATATAGTATGCATT+AGG - chr5_3:46959604-46959623 None:intergenic 30.0%
GTATATATCAGATGCATATG+TGG + chr5_3:46959819-46959838 Msa0814700:intron 30.0%
ATATATCAGATGCATATGTG+GGG + chr5_3:46959821-46959840 Msa0814700:intron 30.0%
GAATATGATGAGGAATTTGA+AGG + chr5_3:46960241-46960260 Msa0814700:CDS 30.0%
TAATGATAACATCGATCGAA+TGG + chr5_3:46960291-46960310 Msa0814700:CDS 30.0%
! TCAACAGTGTCATTACTATT+AGG - chr5_3:46958840-46958859 None:intergenic 30.0%
TTTAATGTGGAGGATAGTTT+AGG - chr5_3:46958915-46958934 None:intergenic 30.0%
AGTAGAAGAGTGATTTAATG+TGG - chr5_3:46958928-46958947 None:intergenic 30.0%
! CAATGGTAAAGTTTTGCTTT+TGG - chr5_3:46958986-46959005 None:intergenic 30.0%
! TGTTGTGTTTTGTGAAATGT+TGG - chr5_3:46959057-46959076 None:intergenic 30.0%
TGATGATCAGGTTTACTAAT+AGG - chr5_3:46959080-46959099 None:intergenic 30.0%
TAGTAAACCTGATCATCATT+TGG + chr5_3:46959082-46959101 Msa0814700:CDS 30.0%
ATCCAATATTGACCTTGAAT+AGG - chr5_3:46959116-46959135 None:intergenic 30.0%
AACGATTCAAGATGGTATTA+AGG - chr5_3:46959466-46959485 None:intergenic 30.0%
! AAAGTGTTAACGATTCAAGA+TGG - chr5_3:46959474-46959493 None:intergenic 30.0%
!!! AGGAGTATTGACAAATTTTC+TGG - chr5_3:46959574-46959593 None:intergenic 30.0%
ATGTCCTAATGCATACTATA+TGG + chr5_3:46959597-46959616 Msa0814700:intron 30.0%
TCTTCCATATAGTATGCATT+AGG - chr5_3:46959604-46959623 None:intergenic 30.0%
GTATATATCAGATGCATATG+TGG + chr5_3:46959819-46959838 Msa0814700:intron 30.0%
ATATATCAGATGCATATGTG+GGG + chr5_3:46959821-46959840 Msa0814700:intron 30.0%
GAATATGATGAGGAATTTGA+AGG + chr5_3:46960241-46960260 Msa0814700:CDS 30.0%
TAATGATAACATCGATCGAA+TGG + chr5_3:46960291-46960310 Msa0814700:CDS 30.0%
AGGTTCAATTGAGGATATGT+AGG - chr5_3:46958820-46958839 None:intergenic 35.0%
AGAAGAGTGATTTAATGTGG+AGG - chr5_3:46958925-46958944 None:intergenic 35.0%
!! TGGTAAAGTTTTGCTTTTGG+TGG - chr5_3:46958983-46959002 None:intergenic 35.0%
! GTGTTTTGTGAAATGTTGGT+TGG - chr5_3:46959053-46959072 None:intergenic 35.0%
CTCCTATTCAAGGTCAATAT+TGG + chr5_3:46959111-46959130 Msa0814700:CDS 35.0%
CAACCGTTTCAATAATATGC+AGG + chr5_3:46959202-46959221 Msa0814700:CDS 35.0%
TGTATCTTGAACTACTAGCT+AGG - chr5_3:46959285-46959304 None:intergenic 35.0%
AGTATGCATTAGGACATTGA+AGG - chr5_3:46959594-46959613 None:intergenic 35.0%
CATGGATCACAATACAGAAA+AGG + chr5_3:46959845-46959864 Msa0814700:CDS 35.0%
AAAAGGACCAGAATCTCTAA+GGG + chr5_3:46959862-46959881 Msa0814700:CDS 35.0%
AGTCTTAACATTGATGAAGC+TGG - chr5_3:46959896-46959915 None:intergenic 35.0%
AGAACACATGAGAAAAACTG+TGG + chr5_3:46960082-46960101 Msa0814700:CDS 35.0%
AGAAAAACTGTGGAAAGCTT+TGG + chr5_3:46960092-46960111 Msa0814700:CDS 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr5_3:46960106-46960125 Msa0814700:CDS 35.0%
AAAGATCATGTTCGTGCTTT+CGG + chr5_3:46960157-46960176 Msa0814700:CDS 35.0%
!! GAAGATCAGGAAGTATTGTT+AGG + chr5_3:46960214-46960233 Msa0814700:CDS 35.0%
GTTAGGTGATGAATATGATG+AGG + chr5_3:46960231-46960250 Msa0814700:CDS 35.0%
! AACATCGATCGAATGGTTTT+CGG + chr5_3:46960298-46960317 Msa0814700:CDS 35.0%
AGGTTCAATTGAGGATATGT+AGG - chr5_3:46958820-46958839 None:intergenic 35.0%
AGAAGAGTGATTTAATGTGG+AGG - chr5_3:46958925-46958944 None:intergenic 35.0%
!! TGGTAAAGTTTTGCTTTTGG+TGG - chr5_3:46958983-46959002 None:intergenic 35.0%
! GTGTTTTGTGAAATGTTGGT+TGG - chr5_3:46959053-46959072 None:intergenic 35.0%
CTCCTATTCAAGGTCAATAT+TGG + chr5_3:46959111-46959130 Msa0814700:CDS 35.0%
CAACCGTTTCAATAATATGC+AGG + chr5_3:46959202-46959221 Msa0814700:CDS 35.0%
TGTATCTTGAACTACTAGCT+AGG - chr5_3:46959285-46959304 None:intergenic 35.0%
AGTATGCATTAGGACATTGA+AGG - chr5_3:46959594-46959613 None:intergenic 35.0%
CATGGATCACAATACAGAAA+AGG + chr5_3:46959845-46959864 Msa0814700:CDS 35.0%
AAAAGGACCAGAATCTCTAA+GGG + chr5_3:46959862-46959881 Msa0814700:CDS 35.0%
AGTCTTAACATTGATGAAGC+TGG - chr5_3:46959896-46959915 None:intergenic 35.0%
AGAACACATGAGAAAAACTG+TGG + chr5_3:46960082-46960101 Msa0814700:CDS 35.0%
AGAAAAACTGTGGAAAGCTT+TGG + chr5_3:46960092-46960111 Msa0814700:CDS 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr5_3:46960106-46960125 Msa0814700:CDS 35.0%
AAAGATCATGTTCGTGCTTT+CGG + chr5_3:46960157-46960176 Msa0814700:CDS 35.0%
!! GAAGATCAGGAAGTATTGTT+AGG + chr5_3:46960214-46960233 Msa0814700:CDS 35.0%
GTTAGGTGATGAATATGATG+AGG + chr5_3:46960231-46960250 Msa0814700:CDS 35.0%
! AACATCGATCGAATGGTTTT+CGG + chr5_3:46960298-46960317 Msa0814700:CDS 35.0%
TCTTCCATTTCACTCTCACT+TGG + chr5_3:46958864-46958883 Msa0814700:CDS 40.0%
! TGGAGGATAGTTTAGGTTTG+TGG - chr5_3:46958908-46958927 None:intergenic 40.0%
!! ACCATTGATGATGAGCAAAG+TGG + chr5_3:46958999-46959018 Msa0814700:CDS 40.0%
ACCACTTTGCTCATCATCAA+TGG - chr5_3:46959003-46959022 None:intergenic 40.0%
GTAACAGTTGCACTTCACAT+TGG + chr5_3:46959023-46959042 Msa0814700:CDS 40.0%
AGCTAGGCAATAGACAGTTT+AGG - chr5_3:46959269-46959288 None:intergenic 40.0%
GAAAAGGACCAGAATCTCTA+AGG + chr5_3:46959861-46959880 Msa0814700:CDS 40.0%
AAAGGACCAGAATCTCTAAG+GGG + chr5_3:46959863-46959882 Msa0814700:CDS 40.0%
TTTACTCCCCTTAGAGATTC+TGG - chr5_3:46959872-46959891 None:intergenic 40.0%
! AGAACAACATAGAGCATCCA+AGG + chr5_3:46959945-46959964 Msa0814700:CDS 40.0%
!! AGTGTTCTGAAATCCTTGAG+TGG - chr5_3:46959977-46959996 None:intergenic 40.0%
CACTTCAAACACACTACAAG+AGG + chr5_3:46959993-46960012 Msa0814700:CDS 40.0%
CTGATCTTCACAACTCTCAA+CGG - chr5_3:46960203-46960222 None:intergenic 40.0%
TCTTCCATTTCACTCTCACT+TGG + chr5_3:46958864-46958883 Msa0814700:CDS 40.0%
! TGGAGGATAGTTTAGGTTTG+TGG - chr5_3:46958908-46958927 None:intergenic 40.0%
!! ACCATTGATGATGAGCAAAG+TGG + chr5_3:46958999-46959018 Msa0814700:CDS 40.0%
ACCACTTTGCTCATCATCAA+TGG - chr5_3:46959003-46959022 None:intergenic 40.0%
GTAACAGTTGCACTTCACAT+TGG + chr5_3:46959023-46959042 Msa0814700:CDS 40.0%
AGCTAGGCAATAGACAGTTT+AGG - chr5_3:46959269-46959288 None:intergenic 40.0%
GAAAAGGACCAGAATCTCTA+AGG + chr5_3:46959861-46959880 Msa0814700:CDS 40.0%
AAAGGACCAGAATCTCTAAG+GGG + chr5_3:46959863-46959882 Msa0814700:CDS 40.0%
TTTACTCCCCTTAGAGATTC+TGG - chr5_3:46959872-46959891 None:intergenic 40.0%
! AGAACAACATAGAGCATCCA+AGG + chr5_3:46959945-46959964 Msa0814700:CDS 40.0%
!! AGTGTTCTGAAATCCTTGAG+TGG - chr5_3:46959977-46959996 None:intergenic 40.0%
CACTTCAAACACACTACAAG+AGG + chr5_3:46959993-46960012 Msa0814700:CDS 40.0%
CTGATCTTCACAACTCTCAA+CGG - chr5_3:46960203-46960222 None:intergenic 40.0%
GCTGAAGCCAAATGATGATC+AGG - chr5_3:46959092-46959111 None:intergenic 45.0%
TTGGCTTCAGCTCCTATTCA+AGG + chr5_3:46959101-46959120 Msa0814700:CDS 45.0%
ATGAGGATTTGAGCAGGAGA+AGG - chr5_3:46959140-46959159 None:intergenic 45.0%
TCTCCTGCTCAAATCCTCAT+TGG + chr5_3:46959140-46959159 Msa0814700:CDS 45.0%
! AGAACTGTGTTGGTCCAATG+AGG - chr5_3:46959157-46959176 None:intergenic 45.0%
ACAGTGCAAGAGAACTGTGT+TGG - chr5_3:46959167-46959186 None:intergenic 45.0%
! ACATAGAGCATCCAAGGTCA+AGG + chr5_3:46959951-46959970 Msa0814700:CDS 45.0%
ACACACTACAAGAGGAAGCA+TGG + chr5_3:46960001-46960020 Msa0814700:CDS 45.0%
! CATTCGGGTGTAGAAAGTGT+GGG + chr5_3:46960032-46960051 Msa0814700:CDS 45.0%
GGGAAACCATTTGCAGTTAG+AGG + chr5_3:46960052-46960071 Msa0814700:CDS 45.0%
CAGTCACCTCTAACTGCAAA+TGG - chr5_3:46960061-46960080 None:intergenic 45.0%
CATTTGCAGTTAGAGGTGAC+TGG + chr5_3:46960059-46960078 Msa0814700:CDS 45.0%
CGTTGAGAGTTGTGAAGATC+AGG + chr5_3:46960201-46960220 Msa0814700:CDS 45.0%
GCTGAAGCCAAATGATGATC+AGG - chr5_3:46959092-46959111 None:intergenic 45.0%
TTGGCTTCAGCTCCTATTCA+AGG + chr5_3:46959101-46959120 Msa0814700:CDS 45.0%
ATGAGGATTTGAGCAGGAGA+AGG - chr5_3:46959140-46959159 None:intergenic 45.0%
TCTCCTGCTCAAATCCTCAT+TGG + chr5_3:46959140-46959159 Msa0814700:CDS 45.0%
! AGAACTGTGTTGGTCCAATG+AGG - chr5_3:46959157-46959176 None:intergenic 45.0%
ACAGTGCAAGAGAACTGTGT+TGG - chr5_3:46959167-46959186 None:intergenic 45.0%
! ACATAGAGCATCCAAGGTCA+AGG + chr5_3:46959951-46959970 Msa0814700:CDS 45.0%
ACACACTACAAGAGGAAGCA+TGG + chr5_3:46960001-46960020 Msa0814700:CDS 45.0%
! CATTCGGGTGTAGAAAGTGT+GGG + chr5_3:46960032-46960051 Msa0814700:CDS 45.0%
GGGAAACCATTTGCAGTTAG+AGG + chr5_3:46960052-46960071 Msa0814700:CDS 45.0%
CAGTCACCTCTAACTGCAAA+TGG - chr5_3:46960061-46960080 None:intergenic 45.0%
CATTTGCAGTTAGAGGTGAC+TGG + chr5_3:46960059-46960078 Msa0814700:CDS 45.0%
CGTTGAGAGTTGTGAAGATC+AGG + chr5_3:46960201-46960220 Msa0814700:CDS 45.0%
!!! TTGTATTTAATTTAAAAAAA+AGG + chr5_3:46959719-46959738 Msa0814700:intron 5.0%
!!! TTGTATTTAATTTAAAAAAA+AGG + chr5_3:46959719-46959738 Msa0814700:intron 5.0%
AGGGCCAAGTGAGAGTGAAA+TGG - chr5_3:46958871-46958890 None:intergenic 50.0%
TGTGGTTGTGTGTTCCAGGA+GGG - chr5_3:46958890-46958909 None:intergenic 50.0%
TTGTGGTTGTGTGTTCCAGG+AGG - chr5_3:46958891-46958910 None:intergenic 50.0%
GGTTTGTGGTTGTGTGTTCC+AGG - chr5_3:46958894-46958913 None:intergenic 50.0%
GGTCCAATGAGGATTTGAGC+AGG - chr5_3:46959146-46959165 None:intergenic 50.0%
CAGATGCATATGTGGGGACA+TGG + chr5_3:46959827-46959846 Msa0814700:intron 50.0%
!! TCCTTGCTATTGCTGTGCAG+AGG + chr5_3:46959916-46959935 Msa0814700:CDS 50.0%
!! CCTTGCTATTGCTGTGCAGA+GGG + chr5_3:46959917-46959936 Msa0814700:CDS 50.0%
AAGCATGGAGCGAAGCCATT+CGG + chr5_3:46960016-46960035 Msa0814700:CDS 50.0%
CCACACTTTCTACACCCGAA+TGG - chr5_3:46960034-46960053 None:intergenic 50.0%
CCATTCGGGTGTAGAAAGTG+TGG + chr5_3:46960031-46960050 Msa0814700:CDS 50.0%
CATGTTCGTGCTTTCGGTGA+TGG + chr5_3:46960163-46960182 Msa0814700:CDS 50.0%
TCACAACTCTCAACGGTGTG+TGG - chr5_3:46960196-46960215 None:intergenic 50.0%
AGGGCCAAGTGAGAGTGAAA+TGG - chr5_3:46958871-46958890 None:intergenic 50.0%
TGTGGTTGTGTGTTCCAGGA+GGG - chr5_3:46958890-46958909 None:intergenic 50.0%
TTGTGGTTGTGTGTTCCAGG+AGG - chr5_3:46958891-46958910 None:intergenic 50.0%
GGTTTGTGGTTGTGTGTTCC+AGG - chr5_3:46958894-46958913 None:intergenic 50.0%
GGTCCAATGAGGATTTGAGC+AGG - chr5_3:46959146-46959165 None:intergenic 50.0%
CAGATGCATATGTGGGGACA+TGG + chr5_3:46959827-46959846 Msa0814700:intron 50.0%
!! TCCTTGCTATTGCTGTGCAG+AGG + chr5_3:46959916-46959935 Msa0814700:CDS 50.0%
!! CCTTGCTATTGCTGTGCAGA+GGG + chr5_3:46959917-46959936 Msa0814700:CDS 50.0%
AAGCATGGAGCGAAGCCATT+CGG + chr5_3:46960016-46960035 Msa0814700:CDS 50.0%
CCACACTTTCTACACCCGAA+TGG - chr5_3:46960034-46960053 None:intergenic 50.0%
CCATTCGGGTGTAGAAAGTG+TGG + chr5_3:46960031-46960050 Msa0814700:CDS 50.0%
CATGTTCGTGCTTTCGGTGA+TGG + chr5_3:46960163-46960182 Msa0814700:CDS 50.0%
TCACAACTCTCAACGGTGTG+TGG - chr5_3:46960196-46960215 None:intergenic 50.0%
CCCTCTGCACAGCAATAGCA+AGG - chr5_3:46959920-46959939 None:intergenic 55.0%
TCCAAGGTCAAGGCCACTCA+AGG + chr5_3:46959961-46959980 Msa0814700:CDS 55.0%
! TCCTTGAGTGGCCTTGACCT+TGG - chr5_3:46959965-46959984 None:intergenic 55.0%
AGCATGGAGCGAAGCCATTC+GGG + chr5_3:46960017-46960036 Msa0814700:CDS 55.0%
CCCTCTGCACAGCAATAGCA+AGG - chr5_3:46959920-46959939 None:intergenic 55.0%
TCCAAGGTCAAGGCCACTCA+AGG + chr5_3:46959961-46959980 Msa0814700:CDS 55.0%
! TCCTTGAGTGGCCTTGACCT+TGG - chr5_3:46959965-46959984 None:intergenic 55.0%
AGCATGGAGCGAAGCCATTC+GGG + chr5_3:46960017-46960036 Msa0814700:CDS 55.0%
TCACTCTCACTTGGCCCTCC+TGG + chr5_3:46958873-46958892 Msa0814700:CDS 60.0%
TCACTCTCACTTGGCCCTCC+TGG + chr5_3:46958873-46958892 Msa0814700:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 46958798 46960327 46958798 ID=Msa0814700;Name=Msa0814700
chr5_3 mRNA 46958798 46960327 46958798 ID=Msa0814700-mRNA-1;Parent=Msa0814700;Name=Msa0814700-mRNA-1;_AED=0.06;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|307
chr5_3 exon 46958798 46959223 46958798 ID=Msa0814700-mRNA-1:exon:14218;Parent=Msa0814700-mRNA-1
chr5_3 exon 46959830 46960327 46959830 ID=Msa0814700-mRNA-1:exon:14219;Parent=Msa0814700-mRNA-1
chr5_3 CDS 46958798 46959223 46958798 ID=Msa0814700-mRNA-1:cds;Parent=Msa0814700-mRNA-1
chr5_3 CDS 46959830 46960327 46959830 ID=Msa0814700-mRNA-1:cds;Parent=Msa0814700-mRNA-1
Gene Sequence

>Msa0814700

ATGCAAGCTCACACATTCTCCTACATATCCTCAATTGAACCTAATAGTAATGACACTGTTGATGAGTCTTCCATTTCACTCTCACTTGGCCCTCCTGGAACACACAACCACAAACCTAAACTATCCTCCACATTAAATCACTCTTCTACTACTCTTCATCATCATCATCATAATCATAATCACCACCAAAAGCAAAACTTTACCATTGATGATGAGCAAAGTGGTGTAACAGTTGCACTTCACATTGGTCTTCCAACCAACATTTCACAAAACACAACACCTATTAGTAAACCTGATCATCATTTGGCTTCAGCTCCTATTCAAGGTCAATATTGGATTCCTTCTCCTGCTCAAATCCTCATTGGACCAACACAGTTCTCTTGCACTGTTTGCAATAAAATGTTCAACCGTTTCAATAATATGCAGATGCATATGTGGGGACATGGATCACAATACAGAAAAGGACCAGAATCTCTAAGGGGAGTAAAACCAGCTTCATCAATGTTAAGACTTCCTTGCTATTGCTGTGCAGAGGGATGCAAGAACAACATAGAGCATCCAAGGTCAAGGCCACTCAAGGATTTCAGAACACTTCAAACACACTACAAGAGGAAGCATGGAGCGAAGCCATTCGGGTGTAGAAAGTGTGGGAAACCATTTGCAGTTAGAGGTGACTGGAGAACACATGAGAAAAACTGTGGAAAGCTTTGGTTTTGTATCTGTGGATCTGATTTCAAACACAAGAGATCTCTTAAAGATCATGTTCGTGCTTTCGGTGATGGTCATGCTCCACACACCGTTGAGAGTTGTGAAGATCAGGAAGTATTGTTAGGTGATGAATATGATGAGGAATTTGAAGGAGATGAAAATTATGAAGATGATGATGATAATGATAACATCGATCGAATGGTTTTCGGTTTTTAA
Protein sequence

>Msa0814700

MQAHTFSYISSIEPNSNDTVDESSISLSLGPPGTHNHKPKLSSTLNHSSTTLHHHHHNHNHHQKQNFTIDDEQSGVTVALHIGLPTNISQNTTPISKPDHHLASAPIQGQYWIPSPAQILIGPTQFSCTVCNKMFNRFNNMQMHMWGHGSQYRKGPESLRGVKPASSMLRLPCYCCAEGCKNNIEHPRSRPLKDFRTLQTHYKRKHGAKPFGCRKCGKPFAVRGDWRTHEKNCGKLWFCICGSDFKHKRSLKDHVRAFGDGHAPHTVESCEDQEVLLGDEYDEEFEGDENYEDDDDNDNIDRMVFGF*