AlfalfaGEDB | Msa0816410

M. sativa cultivar zhongmu-4 / Msa0816410

KEGG: K09422|K16166
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816410	G7K1W1	67.782	239	19	3	4	184	49	287	1.58e-98	296

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TFs/TRs:

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Gene ID	Type	Classification
Msa0816410	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816410	MtrunA17_Chr7g0220341	48.511	235	51	6	10	176	19	251	3.06e-58	183
Msa0816410	MtrunA17_Chr8g0360111	48.085	235	54	7	8	184	17	241	3.25e-46	152
Msa0816410	MtrunA17_Chr7g0229101	61.006	159	48	5	33	184	41	192	1.52e-44	146
Msa0816410	MtrunA17_Chr5g0434931	42.857	245	71	8	6	184	25	266	6.10e-42	142
Msa0816410	MtrunA17_Chr7g0228991	41.633	245	70	9	6	184	19	256	7.55e-39	134
Msa0816410	MtrunA17_Chr5g0434891	46.203	158	48	6	33	176	5	139	8.54e-31	109
Msa0816410	MtrunA17_Chr5g0434911	37.705	244	79	7	7	184	10	246	1.55e-28	107
Msa0816410	MtrunA17_Chr5g0434841	33.803	284	83	8	4	184	8	289	5.53e-26	101
Msa0816410	MtrunA17_Chr5g0434861	57.955	88	33	3	97	182	14	99	3.49e-24	91.7
Msa0816410	MtrunA17_Chr7g0229131	45.000	120	54	4	33	147	41	153	2.42e-22	88.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 37 sgRNAs with CRISPR-Local

Find 668 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTCCATGGACATATG+GGG	0.650224	5_3:+50717595	Msa0816410:CDS
AAGGCTTACATTCACAAATA+TGG	0.336485	5_3:+50717632	Msa0816410:CDS
AATAAGAGAGGCGTGAGAAA+AGG	0.498118	5_3:+50717578	Msa0816410:CDS
ACAAATATGGTGAAGGAAAA+TGG	0.248786	5_3:+50717645	Msa0816410:CDS
ACTATTGGAATACACATCTG+CGG	0.687334	5_3:+50725820	Msa0816410:CDS
AGAAAAGGTCCATGGACATA+TGG	0.400008	5_3:+50717593	Msa0816410:CDS
AGAAGAGAACAACTTTGATA+TGG	0.390818	5_3:+50726184	Msa0816410:CDS
AGAGAACAACTTTGATATGG+AGG	0.585265	5_3:+50726187	Msa0816410:CDS
ATTATGGTTCCAAATCAAAT+TGG	0.344731	5_3:+50726029	Msa0816410:CDS
ATTCATTATACCTGTTCTTT+TGG	0.205114	5_3:-50717678	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCCTATATATATCTGTGTT+TGG	+	chr5_3:50717517-50717536	Msa0816410:intron	30.0%
GTCCAAACACAGATATATAT+AGG	-	chr5_3:50717522-50717541	None:intergenic	30.0%
TATCTGTGTTTGGACAATTA+AGG	+	chr5_3:50717527-50717546	Msa0816410:intron	30.0%
GACAATTAAGGAAATCGTAT+AGG	+	chr5_3:50717539-50717558	Msa0816410:intron	30.0%
TTAAGGAAATCGTATAGGAA+AGG	+	chr5_3:50717544-50717563	Msa0816410:intron	30.0%
AAGGCTTACATTCACAAATA+TGG	+	chr5_3:50717632-50717651	Msa0816410:CDS	30.0%
TACATTCACAAATATGGTGA+AGG	+	chr5_3:50717638-50717657	Msa0816410:CDS	30.0%
ACAAATATGGTGAAGGAAAA+TGG	+	chr5_3:50717645-50717664	Msa0816410:CDS	30.0%
CATTTAATTCCCAAAAGAAC+AGG	+	chr5_3:50717668-50717687	Msa0816410:CDS	30.0%
TGCATGGGATAATATAGATT+TGG	-	chr5_3:50718031-50718050	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	50717449	50726217	50717449	ID=Msa0816410;Name=Msa0816410
chr5_3	mRNA	50717449	50726217	50717449	ID=Msa0816410-mRNA-1;Parent=Msa0816410;Name=Msa0816410-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0\|0\|0\|0.75\|0\|0\|4\|0\|184
chr5_3	exon	50717449	50717452	50717449	ID=Msa0816410-mRNA-1:exon:15232;Parent=Msa0816410-mRNA-1
chr5_3	exon	50717549	50717689	50717549	ID=Msa0816410-mRNA-1:exon:15233;Parent=Msa0816410-mRNA-1
chr5_3	exon	50725805	50726050	50725805	ID=Msa0816410-mRNA-1:exon:15234;Parent=Msa0816410-mRNA-1
chr5_3	exon	50726057	50726217	50726057	ID=Msa0816410-mRNA-1:exon:15235;Parent=Msa0816410-mRNA-1
chr5_3	CDS	50717449	50717452	50717449	ID=Msa0816410-mRNA-1:cds;Parent=Msa0816410-mRNA-1
chr5_3	CDS	50717549	50717689	50717549	ID=Msa0816410-mRNA-1:cds;Parent=Msa0816410-mRNA-1
chr5_3	CDS	50725805	50726050	50725805	ID=Msa0816410-mRNA-1:cds;Parent=Msa0816410-mRNA-1
chr5_3	CDS	50726057	50726217	50726057	ID=Msa0816410-mRNA-1:cds;Parent=Msa0816410-mRNA-1

Gene Sequence

>Msa0816410

ATGAGAAATCGTATAGGAAAGGTGCGTATGGAGAATAAGAGAGGCGTGAGAAAAGGTCCATGGACATATGGGGAAGACAACTTACTGAAGGCTTACATTCACAAATATGGTGAAGGAAAATGGCATTTAATTCCCAAAAGAACAGCTAATGATGTGAAAAACTATTGGAATACACATCTGCGGAAGAAGGTTCTTTCAGAATCATTAGAAAAAAATGAAAAAGAGAGATCTAAGGAAACAATGAAAGCTCATAAAGTTATTAAACCTCGACCTATAACACTTTCAACTCATTCATCTTTCATGACACAACCAATATTGAATTCAAATAAAGATAGTAAAATTCACATGGATCGTCATGACTCATCAGAGATTATGGTTCCAAATCAAATTGATTGTGCTTCTGCTTCACAACTAAGTCTAGGTAATGTTCCCGTACCATGTGGAATGTGGTCGGACAGTTTATGGAACTTGGAAGAACAAGTAAACAGTGACAAAATTGGCTCATGCTCTTCACTACAAGAAGAGAACAACTTTGATATGGAGGTTCCAAAT

Protein sequence

>Msa0816410

MRNRIGKVRMENKRGVRKGPWTYGEDNLLKAYIHKYGEGKWHLIPKRTANDVKNYWNTHLRKKVLSESLEKNEKERSKETMKAHKVIKPRPITLSTHSSFMTQPILNSNKDSKIHMDRHDSSEIMVPNQIDCASASQLSLGNVPVPCGMWSDSLWNLEEQVNSDKIGSCSSLQEENNFDMEVPN