AlfalfaGEDB | Msa0816600

M. sativa cultivar zhongmu-4 / Msa0816600

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816600	XP_003598029.2	45.946	259	90	4	16	225	257	514	6.02e-62	220
Msa0816600	XP_003598029.2	51.724	232	89	2	217	448	2	210	2.04e-64	226
Msa0816600	XP_003598029.2	69.002	471	121	4	1	447	1	470	0.0	639

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816600	sp\|Q9XIB4\|Y1475_ARATH	38.400	125	74	2	5	129	20	141	4.69e-19	88.2
Msa0816600	sp\|Q9XIB4\|Y1475_ARATH	36.036	111	70	1	238	348	32	141	3.56e-14	74.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816600	G7IYE8	45.946	259	90	4	16	225	257	514	2.87e-62	220
Msa0816600	G7IYE8	51.724	232	89	2	217	448	2	210	9.76e-65	226
Msa0816600	G7IYE8	69.002	471	121	4	1	447	1	470	0.0	639

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TFs/TRs:

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Gene ID	Type	Classification
Msa0816600	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816600	MtrunA17_Chr3g0077411	69.002	471	121	4	1	447	1	470	0.0	639
Msa0816600	MtrunA17_Chr3g0077411	51.724	232	89	2	217	448	2	210	1.88e-68	226
Msa0816600	MtrunA17_Chr3g0077411	45.946	259	90	4	16	225	257	514	5.53e-66	220
Msa0816600	MtrunA17_Chr3g0077361	60.460	478	124	4	1	448	20	462	0.0	526
Msa0816600	MtrunA17_Chr3g0077361	48.148	216	105	2	16	225	284	498	1.05e-51	182
Msa0816600	MtrunA17_Chr3g0077461	64.663	416	118	6	36	438	22	421	0.0	522
Msa0816600	MtrunA17_Chr3g0077461	48.945	237	90	4	16	224	232	465	2.23e-65	217
Msa0816600	MtrunA17_Chr4g0009491	61.860	430	135	3	35	445	11	430	0.0	512
Msa0816600	MtrunA17_Chr4g0009491	54.359	195	67	2	254	448	11	183	1.67e-57	195
Msa0816600	MtrunA17_Chr4g0009491	48.544	206	65	1	16	180	220	425	5.39e-55	189

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816600	AT1G49475.1	38.400	125	74	2	5	129	20	141	4.76e-20	88.2
Msa0816600	AT1G49475.1	36.036	111	70	1	238	348	32	141	3.62e-15	74.3
Msa0816600	AT3G18990.1	38.384	99	61	0	21	119	6	104	1.35e-19	90.1
Msa0816600	AT3G18990.1	40.404	99	59	0	240	338	6	104	1.26e-18	87.0
Msa0816600	AT4G01580.1	37.037	108	68	0	14	121	23	130	2.05e-19	86.3
Msa0816600	AT4G01580.1	36.441	118	73	1	225	340	13	130	1.94e-17	80.9
Msa0816600	AT3G18990.2	38.384	99	61	0	21	119	6	104	2.90e-19	89.4
Msa0816600	AT3G18990.2	40.404	99	59	0	240	338	6	104	2.65e-18	86.7
Msa0816600	AT3G18960.2	36.111	108	69	0	14	121	23	130	7.53e-19	84.7
Msa0816600	AT3G18960.2	34.454	119	76	1	224	340	12	130	2.77e-16	77.4

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Find 93 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGAGTTGAAGATATG+TGG	0.601427	5_3:+51104313	Msa0816600:CDS
AAAATTCTCAAGGAAAGCAG+TGG	0.599450	5_3:+51103299	Msa0816600:CDS
AAAATTCTCATGGAGAGCAA+TGG	0.544047	5_3:+51104825	Msa0816600:CDS
AAACTCATACATAACTCCAT+CGG	0.639008	5_3:-51103410	None:intergenic
AAATCAATGCGAATAACGTT+TGG	0.379436	5_3:+51103355	Msa0816600:CDS
AAATGTTTGGAATCCTTCTC+CGG	0.487728	5_3:-51101972	None:intergenic
AAATTCTCAAGGAAAGCAGT+GGG	0.637805	5_3:+51103300	Msa0816600:CDS
AAATTCTCATGGAGAGCAAT+GGG	0.473190	5_3:+51104826	Msa0816600:CDS
AAGACTTTATAACAATATTT+GGG	0.361172	5_3:+51104239	Msa0816600:CDS
AAGGATTGGGAACGAAAAGA+AGG	0.522583	5_3:-51103107	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGTATGTTTGGACATTCA+AGG	+	chr5_3:51102522-51102541	Msa0816600:intron	30.0%
TTAGTGTGTCTTTCATTCTA+TGG	+	chr5_3:51102561-51102580	Msa0816600:intron	30.0%
GACGAGTTTATAACAAGATT+TGG	+	chr5_3:51102819-51102838	Msa0816600:CDS	30.0%
ATTTCCTATCCATTCAAAAC+TGG	+	chr5_3:51103053-51103072	Msa0816600:CDS	30.0%
TTTCCTATCCATTCAAAACT+GGG	+	chr5_3:51103054-51103073	Msa0816600:CDS	30.0%
AAATCAATGCGAATAACGTT+TGG	+	chr5_3:51103355-51103374	Msa0816600:CDS	30.0%
TTGGATTCTTTAGATTTGCA+AGG	+	chr5_3:51103374-51103393	Msa0816600:CDS	30.0%
TGGATTCTTTAGATTTGCAA+GGG	+	chr5_3:51103375-51103394	Msa0816600:CDS	30.0%
AAACTCATACATAACTCCAT+CGG	-	chr5_3:51103413-51103432	None:intergenic	30.0%
ACAGCCTCTTATGTAAAAAT+AGG	+	chr5_3:51103689-51103708	Msa0816600:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	51101933	51104892	51101933	ID=Msa0816600;Name=Msa0816600
chr5_3	mRNA	51101933	51104892	51101933	ID=Msa0816600-mRNA-1;Parent=Msa0816600;Name=Msa0816600-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0.4\|0\|1\|1\|1\|6\|0\|448
chr5_3	exon	51101933	51102037	51101933	ID=Msa0816600-mRNA-1:exon:15347;Parent=Msa0816600-mRNA-1
chr5_3	exon	51102810	51103145	51102810	ID=Msa0816600-mRNA-1:exon:15348;Parent=Msa0816600-mRNA-1
chr5_3	exon	51103241	51103486	51103241	ID=Msa0816600-mRNA-1:exon:15349;Parent=Msa0816600-mRNA-1
chr5_3	exon	51104047	51104121	51104047	ID=Msa0816600-mRNA-1:exon:15350;Parent=Msa0816600-mRNA-1
chr5_3	exon	51104229	51104687	51104229	ID=Msa0816600-mRNA-1:exon:15351;Parent=Msa0816600-mRNA-1
chr5_3	exon	51104767	51104892	51104767	ID=Msa0816600-mRNA-1:exon:15352;Parent=Msa0816600-mRNA-1
chr5_3	CDS	51101933	51102037	51101933	ID=Msa0816600-mRNA-1:cds;Parent=Msa0816600-mRNA-1
chr5_3	CDS	51102810	51103145	51102810	ID=Msa0816600-mRNA-1:cds;Parent=Msa0816600-mRNA-1
chr5_3	CDS	51103241	51103486	51103241	ID=Msa0816600-mRNA-1:cds;Parent=Msa0816600-mRNA-1
chr5_3	CDS	51104047	51104121	51104047	ID=Msa0816600-mRNA-1:cds;Parent=Msa0816600-mRNA-1
chr5_3	CDS	51104229	51104687	51104229	ID=Msa0816600-mRNA-1:cds;Parent=Msa0816600-mRNA-1
chr5_3	CDS	51104767	51104892	51104767	ID=Msa0816600-mRNA-1:cds;Parent=Msa0816600-mRNA-1

Gene Sequence

>Msa0816600

ATGCAGCCTCGCCGGAGAAGATTTGTTCCGTCACCGGCGCCGGAGAAGGATTCCAAACATTTTATGAAAACCATTCTTCCCCCTCCCATGCATACCAAAGAAATAAGAATTCCAGACGAGTTTATAACAAGATTTGGGAATGAACTCAACAGTGTTGCTACGATTACTGTTCCTGACGGTTGCGAATGGCATATGGAAGTGAAGAAATGTGGCAGCGAAGTTTTCTTTTGCAAAAATTGGCAACCGTTCGCAGAACACTACTCTATCTGTTATGGTTGTTATGTAGATTTCAAGTATGAAGGGAATTCAAAGTTTAATGTTGTTATATATGATACAAATTCTGTCGAGATTTCCTATCCATTCAAAACTGGGAAGACTAATGGAGAACCAAACCCAAAAAACCCTTCTTTTCGTTCCCAATCCTTTGACTGTCGATATGCGTATGTTCCTGCTGAATTCGCTGCAAAGTATTTGAAACCAAATGTTCTGTTCAAGCTCCAAAATTCTCAAGGAAAGCAGTGGGAAGTTTATTGTGTATTGAATAAAAGCGGTGGAAAATCAATGCGAATAACGTTTGGATTCTTTAGATTTGCAAGGGAAAACAATCTATCCGATGGAGTTATGTATGAGTTTGAGCTTATCAAGAGGAAGCCGCGTGTTGTGTTACAAGTTACAGCTTCTCACACGGCGGAGAAGGAAGTTAGGGAATCCAAACATTTTAAGAAGGCCATACTTCCGTCACACATTCATGCCAAACAAATTAGAATTCCAGAAGACTTTATAACAATATTTGGGAATGAACTCAAGAATGTTGCTACAGTTACTGTTCCGGATGGTCTAATTTGGAAAATGGAGTTGAAGATATGTGGCAAGGACGTTTTCTTTTGCAACAAATGGCACCAGTTTTCAAAATACTATTCTATAGGATACAGTTGTTACTTGTCTTTCAAATATGAAGGAAATTCAAATTTTAGTGTTATTATATTTGATGCAACATCTGTTGAAATTTGCTATCCATTCAAAACTCCAAGTACTAATGGAGAACCAAACAAAGAGTGTCATAGTCCTAGGAAAAGGTCCAAGGTCGAAACAAGTGAGAGTCAAGAGAAAAAGGTCAAAAACATGTCTAAATATGCTTCTAAAAGGGCTGAAGATGCTGCCAATGCATTCAGCCCAAAAAACCCTTATTTTCGTTCAAAGTGCTCCAAAGGAAGATATGTGTATGTTACTTCTAAATTTGCTTCAAAGTATCTGAAGCCAGATGATCCTATCAAGCTCCAAAATTCTCATGGAGAGCAATGGGAAGTTTCTTGTATATTCTATAACCCCGAAACATCAGCAATGTGA

Protein sequence

>Msa0816600

MQPRRRRFVPSPAPEKDSKHFMKTILPPPMHTKEIRIPDEFITRFGNELNSVATITVPDGCEWHMEVKKCGSEVFFCKNWQPFAEHYSICYGCYVDFKYEGNSKFNVVIYDTNSVEISYPFKTGKTNGEPNPKNPSFRSQSFDCRYAYVPAEFAAKYLKPNVLFKLQNSQGKQWEVYCVLNKSGGKSMRITFGFFRFARENNLSDGVMYEFELIKRKPRVVLQVTASHTAEKEVRESKHFKKAILPSHIHAKQIRIPEDFITIFGNELKNVATVTVPDGLIWKMELKICGKDVFFCNKWHQFSKYYSIGYSCYLSFKYEGNSNFSVIIFDATSVEICYPFKTPSTNGEPNKECHSPRKRSKVETSESQEKKVKNMSKYASKRAEDAANAFSPKNPYFRSKCSKGRYVYVTSKFASKYLKPDDPIKLQNSHGEQWEVSCIFYNPETSAM*