Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820500 | XP_024640285.1 | 89.944 | 537 | 13 | 2 | 231 | 737 | 410 | 935 | 0.0 | 965 |
| Msa0820500 | XP_024640285.1 | 96.121 | 232 | 4 | 1 | 1 | 232 | 1 | 227 | 2.18e-145 | 459 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820500 | sp|Q9ZVC9|FRS3_ARATH | 51.056 | 521 | 203 | 10 | 218 | 736 | 380 | 850 | 2.06e-160 | 488 |
| Msa0820500 | sp|Q9ZVC9|FRS3_ARATH | 37.190 | 242 | 116 | 7 | 1 | 239 | 1 | 209 | 2.80e-30 | 131 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820500 | A0A396HY69 | 89.944 | 537 | 13 | 2 | 231 | 737 | 410 | 935 | 0.0 | 965 |
| Msa0820500 | A0A396HY69 | 96.121 | 232 | 4 | 1 | 1 | 232 | 1 | 227 | 1.04e-145 | 459 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820500 | MtrunA17_Chr5g0440451 | 89.944 | 537 | 13 | 2 | 231 | 737 | 410 | 935 | 0.0 | 965 |
| Msa0820500 | MtrunA17_Chr5g0440451 | 96.121 | 232 | 4 | 1 | 1 | 232 | 1 | 227 | 2.00e-149 | 459 |
| Msa0820500 | MtrunA17_Chr3g0118611 | 59.369 | 507 | 165 | 5 | 231 | 736 | 307 | 773 | 0.0 | 560 |
| Msa0820500 | MtrunA17_Chr3g0118611 | 56.044 | 91 | 39 | 1 | 143 | 232 | 34 | 124 | 5.16e-23 | 104 |
| Msa0820500 | MtrunA17_Chr5g0448511 | 51.636 | 275 | 124 | 3 | 228 | 493 | 193 | 467 | 2.62e-87 | 285 |
| Msa0820500 | MtrunA17_Chr5g0448761 | 50.000 | 262 | 126 | 3 | 231 | 487 | 429 | 690 | 1.10e-76 | 263 |
| Msa0820500 | MtrunA17_Chr5g0448761 | 34.028 | 144 | 78 | 3 | 47 | 173 | 59 | 202 | 1.08e-17 | 87.8 |
| Msa0820500 | MtrunA17_Chr2g0329031 | 35.849 | 265 | 161 | 5 | 236 | 497 | 372 | 630 | 1.51e-41 | 162 |
| Msa0820500 | MtrunA17_Chr3g0080351 | 34.553 | 246 | 155 | 3 | 237 | 480 | 434 | 675 | 1.03e-39 | 157 |
| Msa0820500 | MtrunA17_Chr1g0202341 | 33.208 | 265 | 164 | 3 | 231 | 486 | 413 | 673 | 2.53e-39 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0820500 | AT2G27110.3 | 51.056 | 521 | 203 | 10 | 218 | 736 | 235 | 705 | 3.50e-163 | 488 |
| Msa0820500 | AT2G27110.2 | 51.056 | 521 | 203 | 10 | 218 | 736 | 380 | 850 | 2.09e-161 | 488 |
| Msa0820500 | AT2G27110.2 | 37.190 | 242 | 116 | 7 | 1 | 239 | 1 | 209 | 2.85e-31 | 131 |
| Msa0820500 | AT2G27110.1 | 51.056 | 521 | 203 | 10 | 218 | 736 | 380 | 850 | 2.09e-161 | 488 |
| Msa0820500 | AT2G27110.1 | 37.190 | 242 | 116 | 7 | 1 | 239 | 1 | 209 | 2.85e-31 | 131 |
| Msa0820500 | AT4G38170.1 | 47.082 | 257 | 134 | 1 | 228 | 482 | 200 | 456 | 4.79e-75 | 253 |
| Msa0820500 | AT4G38180.1 | 45.221 | 272 | 144 | 3 | 231 | 497 | 438 | 709 | 1.45e-74 | 257 |
| Msa0820500 | AT4G38180.1 | 32.934 | 167 | 94 | 2 | 24 | 173 | 49 | 214 | 1.25e-20 | 97.8 |
| Msa0820500 | AT4G15090.1 | 36.131 | 274 | 165 | 5 | 231 | 500 | 414 | 681 | 6.14e-45 | 173 |
| Msa0820500 | AT4G15090.5 | 36.131 | 274 | 165 | 5 | 231 | 500 | 414 | 681 | 6.14e-45 | 173 |
Find 202 sgRNAs with CRISPR-Local
Find 437 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTCGGGATCTCATGCTG+AGG | 0.664329 | 5_3:-57871510 | Msa0820500:CDS |
| AAAGTTGCGAGGCGATGATT+AGG | 0.441916 | 5_3:-57871280 | Msa0820500:CDS |
| AACTCGCTGACGAGACACTG+GGG | 0.704142 | 5_3:+57869285 | None:intergenic |
| AACTCGCTGACGAGGCACTG+GGG | 0.710997 | 5_3:+57869351 | None:intergenic |
| AACTCGCTGACGAGGCATTG+GGG | 0.657743 | 5_3:+57869318 | None:intergenic |
| AACTCGGGATCTCATGCTGA+GGG | 0.600343 | 5_3:-57871509 | Msa0820500:CDS |
| AACTTGCTTACGTGCCATTG+GGG | 0.617053 | 5_3:+57869384 | None:intergenic |
| AAGGATGACTGAAAGTTGCG+AGG | 0.682372 | 5_3:-57871291 | Msa0820500:CDS |
| AAGTTAAGTTTCAAGTATCC+AGG | 0.425548 | 5_3:-57867141 | Msa0820500:CDS |
| AATGAATCACGGAAATATGC+TGG | 0.507187 | 5_3:+57870303 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GATGTTCATGTGATAACTAT+CGG | - | chr5_3:57866123-57866142 | Msa0820500:three_prime_UTR | 30.0% | |
| TTTATAGTGAGTATGCTAGA+CGG | - | chr5_3:57866307-57866326 | Msa0820500:three_prime_UTR | 30.0% | |
| ATGATTAGGATAGAGCAAAA+GGG | - | chr5_3:57866453-57866472 | Msa0820500:three_prime_UTR | 30.0% | |
| GGTTGTTACAAAATTTGTGA+AGG | - | chr5_3:57866488-57866507 | Msa0820500:three_prime_UTR | 30.0% | |
| CTGACAATAGTCAAAAGATT+AGG | - | chr5_3:57866670-57866689 | Msa0820500:intron | 30.0% | |
| CCCATTAAAAAGAAGACATT+AGG | - | chr5_3:57866768-57866787 | Msa0820500:intron | 30.0% | |
| TTAAAAAGAAGACATTAGGG+AGG | - | chr5_3:57866772-57866791 | Msa0820500:intron | 30.0% | |
| TAAAAAGAAGACATTAGGGA+GGG | - | chr5_3:57866773-57866792 | Msa0820500:intron | 30.0% | |
| ACAAAATCATTTGACCATGA+TGG | + | chr5_3:57867019-57867038 | None:intergenic | 30.0% | |
| ATAGACAAATATGAACTCAG+AGG | - | chr5_3:57867347-57867366 | Msa0820500:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 57866120 | 57871621 | 57866120 | ID=Msa0820500;Name=Msa0820500 |
| chr5_3 | mRNA | 57866120 | 57871621 | 57866120 | ID=Msa0820500-mRNA-1;Parent=Msa0820500;Name=Msa0820500-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0.5|0.6|1|1|1|5|456|737 |
| chr5_3 | exon | 57866120 | 57866620 | 57866120 | ID=Msa0820500-mRNA-1:exon:17547;Parent=Msa0820500-mRNA-1 |
| chr5_3 | exon | 57867081 | 57867194 | 57867081 | ID=Msa0820500-mRNA-1:exon:17546;Parent=Msa0820500-mRNA-1 |
| chr5_3 | exon | 57868375 | 57868524 | 57868375 | ID=Msa0820500-mRNA-1:exon:17545;Parent=Msa0820500-mRNA-1 |
| chr5_3 | exon | 57869165 | 57870374 | 57869165 | ID=Msa0820500-mRNA-1:exon:17544;Parent=Msa0820500-mRNA-1 |
| chr5_3 | exon | 57870927 | 57871621 | 57870927 | ID=Msa0820500-mRNA-1:exon:17543;Parent=Msa0820500-mRNA-1 |
| chr5_3 | CDS | 57870927 | 57871621 | 57870927 | ID=Msa0820500-mRNA-1:cds;Parent=Msa0820500-mRNA-1 |
| chr5_3 | CDS | 57869165 | 57870374 | 57869165 | ID=Msa0820500-mRNA-1:cds;Parent=Msa0820500-mRNA-1 |
| chr5_3 | CDS | 57868375 | 57868524 | 57868375 | ID=Msa0820500-mRNA-1:cds;Parent=Msa0820500-mRNA-1 |
| chr5_3 | CDS | 57867081 | 57867194 | 57867081 | ID=Msa0820500-mRNA-1:cds;Parent=Msa0820500-mRNA-1 |
| chr5_3 | CDS | 57866576 | 57866620 | 57866576 | ID=Msa0820500-mRNA-1:cds;Parent=Msa0820500-mRNA-1 |
| chr5_3 | three_prime_UTR | 57866120 | 57866575 | 57866120 | ID=Msa0820500-mRNA-1:three_prime_utr;Parent=Msa0820500-mRNA-1 |
| Gene Sequence |
>Msa0820500 ATGGATGTTCATGTGATAACTATCGGAGAAGGAAGTGGATCGATGGAACATAATGTGGATGCTGAATTTGGTCAAGTTAATGATGCTGGAAACTCGGGATCTCATGCTGAGGGTATGGTTTCGGAGCCATATTTTTCGGAGCCGTATTTGGGCATGGTATTTGATTCTGAAGATTCTGCTAAAAGTTTTTATAGTGAGTATGCTAGACGGTCGGGTTTCAGCTCAAAAGCTGGTTTGCACGGTCAGTTTAATGCTGATGGGACAAATATGTTCCGGGAGTTTGTGTGTAGCAGGGAAGGTTTGAAAAGAAGGATGACTGAAAGTTGCGAGGCGATGATTAGGATAGAGCAAAAGGGTCAAAATAAGTGGGTTGTTACAAAATTTGTGAAGGAACATAGCCATTCTCTGGCGGTACCTGATAAGGTGGAAAAACTTCAACCAGTGAAGCACTTTTCTAGTGTTGGAAGAACCATGCCTGAAACTTACAAAGGACTGGGGCTTGTTCCAACTGGTGTCATGTATATATCTATTGATGCGCATCACGTTTCCTCTGACAATAGTCAAAAGATTAGGAATTCTCCTGCAGCTGCCGCGAAAACGAGTCATCCAGTTAAAAATTCCACTTCGATGCCTTATAATGTGAGGCAACCCATTAAAAAGAAGACATTAGGGAGGGATGCTCGGGACCTGCTGGAAGGGCATGATTGGCTTCAGTCCTTGTATAGTGCTCGTGATCAATGGGTTCCAGCATATTTCCGTGATTCATTTTTCGCTGTAATATCTCCTACTCAAGGATTTGAAGCCTCTTTTTTGGACGGCGTTGTCAACCGGCATACAACATTACCAATGTTCTTTAGGCAGTATGAACTAGCTCTGGAGAAATGGTTTGAAAAGGAAATAGAATCAGATTTTGAGACAATTAGCACTAATCCAGTTCTAAAGACACCTTCGCCAATGGAGAATCAGGTTGCTAAGCTTTATACACAGAGAATATTTTTGAAGTTTCAAGAAGAGCTAGTTGAAACTTTTGCTTATACCGCCAACAGAATCGAAGAGGATGGGGTGAATAGCATATTTGGAGTTGCAAAGTTTGATGATGTCGAAAAGTCGTACACTGTCACATTAAACCAGTCTGAATTGAGAGCTAACTGTACATGCCAAATGTTTGAGTTTTCAGGCATTCTTTGTAGGCACATTTTAACTGTTTTTACCGTGTCTAATGTATTCACTATACCATCCCACTACATCTTAAAACGGTGGACAATGGAAGCAAAAAACAGTATTGTATTGGATGAATGTGGTGGTGAATCACTTGCATATGAGTCTTTGACATCACGGTATGCCAATCTGTGCCGGGAAGCCATCAGATATGCTGAGGAAGGTGCTGTAGCTATGGAAACTTATGATGCTGCAATGACTGGTCTTAGAGAAGGTGGAAAGAAAGTTGATGTCATGAAGAGAAATATTGCCAAAGTGTCATCAACTAATCATCGACTCAGCTGGAATGATTGCTCCGATCCCGACAAGAGAACCCCTACTTCAACATCAGATACAAGTACAACCCCTCTTTTATGGCCACTTAACGATGAAACATCGACGTTTAATCTTAATGACGTCGATGCTCCTGTTCAACCAATGGCTGATCTGAATTTACCACAAACGACCCCAATGGCACGTAAGCAAGTTGATGTTCCATCCCCAGTGCCTCGTCAGCGAGTTGAGAGTCCATCCCCAATGCCTCGTCAGCGAGTTGATAGTCCATCCCCAGTGTCTCGTCAGCGAGTTGATGGTCCACGACCAGTGCCTCGTAGGCGAGTTGATGGTCCATCCCCAATGCCTCGTCAGCGAGATGATGGTCCGCCCCCAGCGCCTCTTCAGCAAGATGATGGTCCACCTGAAAACACGTTGCAAGATTACAACAAGAGTCCATCAACAGAATATGAGGTTAAGTTCCATCTCTCTCGGGTTACTGTGGGACCTGTTTTGAAGTCTATGACTGAAGTCGGGGAACAACTTTCAACAACAGCAAATAAGGTTGCTGTAATAAATCTCAAGCTTGATAATGCCCGGGTTTCTGGCGAGTCTGAAGTTAAGTTTCAAGTATCCAGGGATGCATTGGGTGCTGTGCTACGATCAATGGCTTATATCCGCGAGCAACTTTCTTGTGCTGGCGACGCGAACTCAGAACCTATGTCAAAAAAGCGTCGAAACTGA |
| Protein sequence |
>Msa0820500 MDVHVITIGEGSGSMEHNVDAEFGQVNDAGNSGSHAEGMVSEPYFSEPYLGMVFDSEDSAKSFYSEYARRSGFSSKAGLHGQFNADGTNMFREFVCSREGLKRRMTESCEAMIRIEQKGQNKWVVTKFVKEHSHSLAVPDKVEKLQPVKHFSSVGRTMPETYKGLGLVPTGVMYISIDAHHVSSDNSQKIRNSPAAAAKTSHPVKNSTSMPYNVRQPIKKKTLGRDARDLLEGHDWLQSLYSARDQWVPAYFRDSFFAVISPTQGFEASFLDGVVNRHTTLPMFFRQYELALEKWFEKEIESDFETISTNPVLKTPSPMENQVAKLYTQRIFLKFQEELVETFAYTANRIEEDGVNSIFGVAKFDDVEKSYTVTLNQSELRANCTCQMFEFSGILCRHILTVFTVSNVFTIPSHYILKRWTMEAKNSIVLDECGGESLAYESLTSRYANLCREAIRYAEEGAVAMETYDAAMTGLREGGKKVDVMKRNIAKVSSTNHRLSWNDCSDPDKRTPTSTSDTSTTPLLWPLNDETSTFNLNDVDAPVQPMADLNLPQTTPMARKQVDVPSPVPRQRVESPSPMPRQRVDSPSPVSRQRVDGPRPVPRRRVDGPSPMPRQRDDGPPPAPLQQDDGPPENTLQDYNKSPSTEYEVKFHLSRVTVGPVLKSMTEVGEQLSTTANKVAVINLKLDNARVSGESEVKFQVSRDALGAVLRSMAYIREQLSCAGDANSEPMSKKRRN* |
