AlfalfaGEDB | Msa0820920

M. sativa cultivar zhongmu-4 / Msa0820920

KEGG: -
GO: -
PFAM: PF13921.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820920	XP_003617168.2	45.294	340	108	7	15	315	8	308	2.61e-65	229
Msa0820920	XP_003617168.2	81.232	357	33	4	320	644	1	355	3.05e-176	515

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820920	sp\|Q9SM27\|MY104_ARATH	51.220	123	58	1	323	443	9	131	1.63e-33	135
Msa0820920	sp\|Q9SM27\|MY104_ARATH	39.444	180	78	5	3	164	3	169	4.60e-27	117

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820920	G7K7T1	45.294	340	108	7	15	315	8	308	1.25e-65	229
Msa0820920	G7K7T1	81.232	357	33	4	320	644	1	355	1.46e-176	515

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TFs/TRs:

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Gene ID	Type	Classification
Msa0820920	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820920	MtrunA17_Chr5g0441131	88.679	159	13	2	320	475	1	157	1.03e-90	279
Msa0820920	MtrunA17_Chr5g0441131	51.456	206	52	4	15	201	8	184	8.55e-49	169
Msa0820920	MtrunA17_Chr5g0440761	52.033	246	85	7	331	556	13	245	1.56e-63	211
Msa0820920	MtrunA17_Chr5g0440761	43.377	302	89	8	17	296	13	254	1.34e-59	200
Msa0820920	MtrunA17_Chr5g0441141	48.188	276	102	9	331	578	13	275	2.81e-63	210
Msa0820920	MtrunA17_Chr5g0441141	41.456	316	96	8	17	296	13	275	1.19e-57	196
Msa0820920	MtrunA17_Chr5g0440781	68.919	148	40	2	331	475	13	157	7.93e-61	201
Msa0820920	MtrunA17_Chr5g0440781	53.140	207	44	6	17	201	13	188	1.12e-54	184
Msa0820920	MtrunA17_Chr1g0192411	43.694	222	82	6	6	203	2	204	3.89e-39	149
Msa0820920	MtrunA17_Chr1g0192411	55.963	109	46	1	330	436	12	120	1.67e-30	124

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820920	AT2G26950.1	51.220	123	58	1	323	443	9	131	1.66e-34	135
Msa0820920	AT2G26950.1	39.444	180	78	5	3	164	3	169	4.68e-28	117
Msa0820920	AT2G32460.2	53.390	118	37	1	16	115	6	123	2.34e-33	134
Msa0820920	AT2G32460.2	43.709	151	69	4	328	474	4	142	1.43e-32	132
Msa0820920	AT2G32460.1	53.390	118	37	1	16	115	18	135	2.35e-33	134
Msa0820920	AT2G32460.1	43.709	151	69	4	328	474	16	154	1.65e-32	132
Msa0820920	AT2G26960.1	46.528	144	58	2	6	131	10	152	3.93e-33	132
Msa0820920	AT2G26960.1	46.610	118	61	1	328	443	18	135	2.53e-30	124
Msa0820920	AT5G55020.1	52.419	124	38	2	8	113	21	141	1.07e-32	132
Msa0820920	AT5G55020.1	50.877	114	52	2	330	440	26	138	2.56e-30	125

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Find 115 sgRNAs with CRISPR-Local

Find 710 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATGGAACCAGAAATT+GGG	0.497571	5_3:+58597702	Msa0820920:CDS
AAAAGAAAACTTGAAAGACA+TGG	0.648245	5_3:+58589421	Msa0820920:CDS
AAACATGATGATGAGTTGAA+TGG	0.543144	5_3:+58589469	Msa0820920:CDS
AAAGTTCTTGATGTCATTAT+CGG	0.301665	5_3:-58598227	None:intergenic
AAATATGCTATACAATAATG+TGG	0.534435	5_3:+58589705	Msa0820920:CDS
AAATCATGCTCCAAAGAAAC+TGG	0.398721	5_3:-58598972	None:intergenic
AAATTTGGAAGCTGATGTTT+TGG	0.296781	5_3:-58589670	None:intergenic
AACACGGGACTTGCACACTT+TGG	0.468274	5_3:+58597739	Msa0820920:CDS
AACTATGTATGGAAAAGGGG+AGG	0.665968	5_3:+58598947	Msa0820920:CDS
AAGAGAAAACATGAAAAGCG+TGG	0.670550	5_3:+58598262	Msa0820920:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAGCATATGTGACAAAATA+TGG	+	chr5_3:58587846-58587865	Msa0820920:CDS	30.0%
TATGTGACAAAATATGGAGT+CGG	+	chr5_3:58587852-58587871	Msa0820920:CDS	30.0%
CTGCAACCAGATTTAAAAAA+GGG	+	chr5_3:58587951-58587970	Msa0820920:CDS	30.0%
AGTCTAAAAAAGTGACATAG+AGG	+	chr5_3:58588220-58588239	Msa0820920:intron	30.0%
ACTGTAAAACTTCATTGTGA+AGG	+	chr5_3:58588407-58588426	Msa0820920:intron	30.0%
TAACCATGCCTACAAAATTA+AGG	+	chr5_3:58588444-58588463	Msa0820920:intron	30.0%
ATAATGTGTATCCAAATGCA+TGG	-	chr5_3:58588861-58588880	None:intergenic	30.0%
AATATACCAGAGGTGAAATT+TGG	+	chr5_3:58589559-58589578	Msa0820920:intron	30.0%
TTTATCTCGTCACAAATTGA+TGG	-	chr5_3:58589610-58589629	None:intergenic	30.0%
TGATATGGGACATATGTAAA+AGG	+	chr5_3:58589902-58589921	Msa0820920:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	58587722	58599118	58587722	ID=Msa0820920;Name=Msa0820920
chr5_3	mRNA	58587722	58599118	58587722	ID=Msa0820920-mRNA-1;Parent=Msa0820920;Name=Msa0820920-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|0.9\|0.22\|0.1\|10\|0\|644
chr5_3	exon	58587722	58587727	58587722	ID=Msa0820920-mRNA-1:exon:17812;Parent=Msa0820920-mRNA-1
chr5_3	exon	58587765	58588001	58587765	ID=Msa0820920-mRNA-1:exon:17813;Parent=Msa0820920-mRNA-1
chr5_3	exon	58589373	58589531	58589373	ID=Msa0820920-mRNA-1:exon:17814;Parent=Msa0820920-mRNA-1
chr5_3	exon	58589571	58589773	58589571	ID=Msa0820920-mRNA-1:exon:17815;Parent=Msa0820920-mRNA-1
chr5_3	exon	58590564	58590803	58590564	ID=Msa0820920-mRNA-1:exon:17816;Parent=Msa0820920-mRNA-1
chr5_3	exon	58590816	58590917	58590816	ID=Msa0820920-mRNA-1:exon:17817;Parent=Msa0820920-mRNA-1
chr5_3	exon	58597606	58597843	58597606	ID=Msa0820920-mRNA-1:exon:17818;Parent=Msa0820920-mRNA-1
chr5_3	exon	58597853	58597900	58597853	ID=Msa0820920-mRNA-1:exon:17819;Parent=Msa0820920-mRNA-1
chr5_3	exon	58598214	58598404	58598214	ID=Msa0820920-mRNA-1:exon:17820;Parent=Msa0820920-mRNA-1
chr5_3	exon	58598608	58599118	58598608	ID=Msa0820920-mRNA-1:exon:17821;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58587722	58587727	58587722	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58587765	58588001	58587765	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58589373	58589531	58589373	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58589571	58589773	58589571	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58590564	58590803	58590564	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58590816	58590917	58590816	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58597606	58597843	58597606	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58597853	58597900	58597853	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58598214	58598404	58598214	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1
chr5_3	CDS	58598608	58599118	58598608	ID=Msa0820920-mRNA-1:cds;Parent=Msa0820920-mRNA-1

Gene Sequence

>Msa0820920

AAGCACCAAGAAAAAGCCAACGACGGTGGTGATATGAATGATGAGTTGAAAAAAGGAATATGGTCAAAAGATGAGGATGCAATATTAGAAGCATATGTGACAAAATATGGAGTCGGAAATTGGAATGCAATATCAAAAAATGCAGGGCTTAAACGTTGTGGAAAAAGTTGTCGTTTTAGATGGTATAATCACCTGCAACCAGATTTAAAAAAGGGACCATTTAGTGAAGAAGAAGAAAAAAAATTGCCTGGAAGAACAGATAATGCGATAAAAAACTTTTGGAATGCAAGAAAAAGAAAACTTGAAAGACATGGCTTGTCACCCTACCCTCCTAGCTTAAAACATGATGATGAGTTGAATGGTAATGGAGAAAGTAGTTTGAGTGGTTCCAGCAACCAACAAGTGAAATTTGGAAAAGATTTTTTGGGTAGAAAATATCCATCAATTTGTGACGAGATAAATGAAAACAACGTTGAAAAATGTTCAAACTTATTTGATGTCCCAAAACATCAGCTTCCAAATTTTTCTGATGATTCAAATATGCTATACAATAATGTGGGAAGTACTTCCATAAGCAATCATACCACCCCAACTTTGATTGACAGTGAGTATCCGCAAAATCCACCACCTAAGGAGACTCAATATCATATGCCAGAATCAATTGCTCCACATAGTAGTGGTTCATTGCAGTCCATTTTCTATACACCAAAGATTTCGGAAGGCTCAGATGCTAGTCGAATGCATTCCTCTGAGATAAATCTAGTTGATAAAAAAGGGAAGAGGCCAATTTCGTTCGAGAATAATGTTGGAGAAAACCGTAGCAACTTTATAGATTTGGATCTTTTTTCATTAGATAATGAAAATAACAATAACTTGGATGACAAGGTTCCACACGATATAAAGGATACCTTAGATGCAGTTATATATGGAAAAAGCTTCCATCCTAATATCATCATCATGTCTTCATTATCGGGCACTAATAAAGGGTTGAAAAAAGAAAAGTGGTCAAAAGAAGAGGATGAAATATTAAAAGCATATGTGGAAAAACATGGAACCAGAAATTGGGACGAGGTATCAAAGAACACGGGACTTGCACACTTTGGAAGAAGTTGCCGTTTTAGATGGTATAATAACCTACAACCTGGTGTAAAAAAGGGTCCATTTAGTAAAGAAGAAGAAAATAAATTACGTAAGAAGTTTGGTGGATTCAAATGGTCTAAAATCGCTCGCGAGTTGCCTGGAAGAACCGATAATGACATCAAGAACTTTTGGAATGCGAGAAAGAGAAAACATGAAAAGCGTGGCTTGTCACCCTTCCCTAACAACATGGAACTGGATGATGAGTTGAATGGTGGTTCCAGTGGCCAACAAGGTGAGGATTGTCAAGAAAATAACTTTGATGTCTCGAATAAGCAGTTTAAAAAGGGTGGCGCTGGAATTTCACCTATACTTTTGACTATGCAATCTGATCTGCCTCCACTCCCTCAGAATCCACCACCTATGGAGACTCAATATCAGATGTCAGAATCAATTTCTCCATATACAGAAGAGAATTGGGAAGGTTTGGATGCTAGTCGCGTGCATTCCTCTGAGATAAATTTTATTGATGAAGAAGGTAAGAAGCCAATTTCTTTGGAGAATGATGTTGAAGACCATGACGACTTAATTGCTATAGATATTTGGTTAAACTATGACTTAAATGAAGAAGACATTCACAACTTACATGACAAGCTTCAGCATGATGTAAATGATTCCTTGTACACAACTATGTATGGAAAAGGGGAGGAGCCAGTTTCTTTGGAGCATGATTTTGAAGTAAACCATGGCGACTTAATGAATCCAAAACTTTGGGTTGACATCAACGAATTACTCAATGACGATGACAATAACTCTGATCATGACAAGCTTCTACAACTATATATGGAGAAGGCATAG

Protein sequence

>Msa0820920

KHQEKANDGGDMNDELKKGIWSKDEDAILEAYVTKYGVGNWNAISKNAGLKRCGKSCRFRWYNHLQPDLKKGPFSEEEEKKLPGRTDNAIKNFWNARKRKLERHGLSPYPPSLKHDDELNGNGESSLSGSSNQQVKFGKDFLGRKYPSICDEINENNVEKCSNLFDVPKHQLPNFSDDSNMLYNNVGSTSISNHTTPTLIDSEYPQNPPPKETQYHMPESIAPHSSGSLQSIFYTPKISEGSDASRMHSSEINLVDKKGKRPISFENNVGENRSNFIDLDLFSLDNENNNNLDDKVPHDIKDTLDAVIYGKSFHPNIIIMSSLSGTNKGLKKEKWSKEEDEILKAYVEKHGTRNWDEVSKNTGLAHFGRSCRFRWYNNLQPGVKKGPFSKEEENKLRKKFGGFKWSKIARELPGRTDNDIKNFWNARKRKHEKRGLSPFPNNMELDDELNGGSSGQQGEDCQENNFDVSNKQFKKGGAGISPILLTMQSDLPPLPQNPPPMETQYQMSESISPYTEENWEGLDASRVHSSEINFIDEEGKKPISLENDVEDHDDLIAIDIWLNYDLNEEDIHNLHDKLQHDVNDSLYTTMYGKGEEPVSLEHDFEVNHGDLMNPKLWVDINELLNDDDNNSDHDKLLQLYMEKA*