AlfalfaGEDB | Msa0820950

M. sativa cultivar zhongmu-4 / Msa0820950

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820950	AES92029.1	80.311	193	17	2	29	200	31	223	3.36e-107	317

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820950	G7JQ00	80.311	193	17	2	29	200	31	223	1.60e-107	317

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TFs/TRs:

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Gene ID	Type	Classification
Msa0820950	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820950	MtrunA17_Chr5g0393501	32.308	195	108	4	29	199	45	239	8.37e-28	105
Msa0820950	MtrunA17_Chr4g0044671	27.222	180	110	2	29	187	28	207	4.26e-14	68.2
Msa0820950	MtrunA17_Chr4g0034781	26.667	180	111	2	29	187	22	201	1.53e-13	66.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 53 sgRNAs with CRISPR-Local

Find 317 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCGTGATGTTTCACTCTA+AGG	0.341127	5_3:+58640258	None:intergenic
ACACGATGCTGCCGCACTCA+TGG	0.487735	5_3:+58640459	None:intergenic
ACCCTATCATTCCATGAGTG+CGG	0.640367	5_3:-58640470	Msa0820950:CDS
ACGGAAATGATATTGTCATA+AGG	0.522697	5_3:+58640218	None:intergenic
AGATTTGCGATTTCCAATTT+GGG	0.327058	5_3:-58642432	Msa0820950:CDS
AGCCGTTGTTGACAGATGGT+TGG	0.550734	5_3:-58640612	Msa0820950:CDS
AGGAATTTGCCGAAAGTATC+AGG	0.335653	5_3:+58640278	None:intergenic
AGTCAGCCGTTGTTGACAGA+TGG	0.548377	5_3:-58640616	Msa0820950:CDS
ATCTACTTCCACCAAGGTAC+TGG	0.428620	5_3:+58640539	None:intergenic
ATGTGATGTTCGGAAAAGGT+TGG	0.606400	5_3:-58640163	Msa0820950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATTTGCGATTTCCAATTT+GGG	-	chr5_3:58640117-58640136	Msa0820950:CDS	30.0%
TGGTCCCTTAAGTATTTATT+TGG	-	chr5_3:58640227-58640246	Msa0820950:CDS	30.0%
ATTTCTCTTTCCTCTTACTT+CGG	+	chr5_3:58640518-58640537	None:intergenic	30.0%
AGAAAATCTAGAGAGTCATA+GGG	+	chr5_3:58640879-58640898	None:intergenic	30.0%
TATGCTATGAAAAATAGGGA+TGG	+	chr5_3:58640990-58641009	None:intergenic	30.0%
AATCATTGGGAATAGGAAAA+TGG	-	chr5_3:58641099-58641118	Msa0820950:intron	30.0%
TCATTAGTACATGTTTAGGA+GGG	+	chr5_3:58641219-58641238	None:intergenic	30.0%
TTGATTAGCAATTAGTGTAG+GGG	+	chr5_3:58641380-58641399	None:intergenic	30.0%
ATCTTCCTATCACTCTATTT+CGG	+	chr5_3:58641546-58641565	None:intergenic	30.0%
ATAGGAAGATATGCGTTTAA+AGG	-	chr5_3:58641556-58641575	Msa0820950:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	58640070	58642501	58640070	ID=Msa0820950;Name=Msa0820950
chr5_3	mRNA	58640070	58642501	58640070	ID=Msa0820950-mRNA-1;Parent=Msa0820950;Name=Msa0820950-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0\|0\|0\|0.75\|0\|0\|4\|0\|200
chr5_3	exon	58642417	58642501	58642417	ID=Msa0820950-mRNA-1:exon:17842;Parent=Msa0820950-mRNA-1
chr5_3	exon	58640604	58640745	58640604	ID=Msa0820950-mRNA-1:exon:17841;Parent=Msa0820950-mRNA-1
chr5_3	exon	58640444	58640579	58640444	ID=Msa0820950-mRNA-1:exon:17840;Parent=Msa0820950-mRNA-1
chr5_3	exon	58640070	58640306	58640070	ID=Msa0820950-mRNA-1:exon:17839;Parent=Msa0820950-mRNA-1
chr5_3	CDS	58642417	58642501	58642417	ID=Msa0820950-mRNA-1:cds;Parent=Msa0820950-mRNA-1
chr5_3	CDS	58640604	58640745	58640604	ID=Msa0820950-mRNA-1:cds;Parent=Msa0820950-mRNA-1
chr5_3	CDS	58640444	58640579	58640444	ID=Msa0820950-mRNA-1:cds;Parent=Msa0820950-mRNA-1
chr5_3	CDS	58640070	58640306	58640070	ID=Msa0820950-mRNA-1:cds;Parent=Msa0820950-mRNA-1

Gene Sequence

>Msa0820950

ATTTGGGTAAGGGGTTTGCGATTTGGGGAAGAGGTGGAGAAGGTTCCAGATTTGCGATTTCCAATTTGGGGAAGAGGAGGAGAAGAACTACAACAATTATTCGTTGCTAAATATTTGTACGAGCTACCGGAGCAATGGCACTTGCTAGATGGTGATGGGAATACTCATAACGTCATGTTCAACAAATCAGTTAGTCAGCCGTTGTTGACAGATGGTTGGAGTGCATTAAGTAAGATTATTGCTTTCCAGTACCTTGGTGGAAGTAGATTTCAAATCTTTGTTTTTGCTCATAACATTGCTCCAGCTGATTATCCACCCTATCATTCCATGAGTGCGGCAGCATCGTGTTATTGTACCTTTGAAGAACTACCTGATACTTTCGGCAAATTCCTTAGAGTGAAACATCACGATTACGTCATGTTGTGCAGACCTTATGACAATATCATTTCCGTGAGACTCAAATATACAGACGACCCCATACCAAATGTGATGTTCGGAAAAGGTTGGACGAGTTTTTGTGCTGCCAATGGTATTGAGGGCGGAGACGTGTTGATTTTCAAGTGCAAGTGCGTCATGGACAAAAAATATGTTATCGTATCC

Protein sequence

>Msa0820950

IWVRGLRFGEEVEKVPDLRFPIWGRGGEELQQLFVAKYLYELPEQWHLLDGDGNTHNVMFNKSVSQPLLTDGWSALSKIIAFQYLGGSRFQIFVFAHNIAPADYPPYHSMSAAASCYCTFEELPDTFGKFLRVKHHDYVMLCRPYDNIISVRLKYTDDPIPNVMFGKGWTSFCAANGIEGGDVLIFKCKCVMDKKYVIVS