AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0825370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0825370 MtrunA17_Chr5g0447111 95.152 330 14 1 1 328 1 330 0.0 650
Msa0825370 MtrunA17_Chr3g0103031 64.438 329 114 3 1 327 1 328 1.03e-152 431
Msa0825370 MtrunA17_Chr3g0124921 46.894 322 166 4 5 323 2 321 4.69e-99 295
Msa0825370 MtrunA17_Chr1g0159831 44.954 327 173 4 7 327 5 330 7.25e-99 295
Msa0825370 MtrunA17_Chr5g0447191 54.335 173 78 1 152 323 15 187 5.53e-60 190
Msa0825370 MtrunA17_Chr4g0029841 33.482 224 125 5 98 321 77 276 3.64e-29 113
Msa0825370 MtrunA17_Chr3g0114411 33.537 164 105 3 133 292 70 233 1.52e-13 69.7
Msa0825370 MtrunA17_Chr7g0259501 28.000 150 96 3 153 291 197 345 2.29e-12 67.4
Msa0825370 MtrunA17_Chr5g0394261 29.078 141 97 3 163 302 536 674 3.52e-12 67.4
Msa0825370 MtrunA17_Chr7g0259851 29.231 130 90 2 163 292 526 653 4.24e-12 67.0
Msa0825370 MtrunA17_Chr1g0185861 27.815 151 97 3 153 292 201 350 8.32e-12 65.9
Msa0825370 MtrunA17_Chr3g0125321 28.289 152 103 3 174 323 94 241 8.78e-12 64.3
Msa0825370 MtrunA17_Chr5g0440341 29.487 156 90 4 153 292 191 342 2.65e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0825370 AT1G55760.1 57.317 328 138 2 1 327 1 327 2.69e-138 395
Msa0825370 AT1G21780.2 48.137 322 164 3 7 327 5 324 3.01e-110 324
Msa0825370 AT1G21780.1 48.137 322 164 3 7 327 5 324 3.01e-110 324
Msa0825370 AT1G01640.1 29.787 188 128 4 142 327 3 188 1.95e-15 74.3
Msa0825370 AT3G56230.1 29.870 154 105 3 142 292 90 243 3.99e-14 72.0
Msa0825370 AT2G39760.2 28.947 152 96 2 151 291 182 332 1.08e-13 71.2
Msa0825370 AT2G39760.4 28.947 152 96 2 151 291 182 332 1.14e-13 71.2
Msa0825370 AT2G39760.3 28.947 152 96 2 151 291 182 332 1.14e-13 71.2
Msa0825370 AT1G01640.2 29.586 169 115 4 161 327 1 167 2.09e-13 68.2
Msa0825370 AT2G39760.1 28.947 152 96 2 151 291 182 332 2.64e-13 70.5
Msa0825370 AT1G05690.1 26.415 159 108 4 149 301 40 195 1.79e-11 65.1
Msa0825370 AT5G63160.2 28.824 170 114 4 161 325 23 190 3.74e-11 63.5
Msa0825370 AT5G63160.1 28.824 170 114 4 161 325 23 190 4.53e-11 63.5
Msa0825370 AT3G06190.5 27.632 152 98 3 151 291 190 340 7.98e-11 62.8
Msa0825370 AT2G30600.4 28.671 143 97 3 138 275 326 468 9.73e-11 63.5
Msa0825370 AT2G30600.1 28.671 143 97 3 138 275 326 468 9.73e-11 63.5
Msa0825370 AT2G30600.3 28.671 143 97 3 138 275 326 468 9.73e-11 63.5
Msa0825370 AT2G30600.2 28.671 143 97 3 138 275 326 468 9.73e-11 63.5

Find 59 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATTGACATTGAGTTTC+TGG 0.099574 5_3:+65148513 Msa0825370:CDS
ATGCAATATCTTGTGAAATT+TGG 0.204904 5_3:+65149296 Msa0825370:CDS
TGGCAAAATATATGATATTC+AGG 0.211529 5_3:+65149316 Msa0825370:CDS
TGGATAGGTTGTTCGGTTAT+TGG 0.221135 5_3:+65149475 Msa0825370:three_prime_UTR
ATTGATACAAAGTTGCAATT+TGG 0.261172 5_3:-65149250 None:intergenic
GTGGGGAAGCTTGCTCTATT+TGG 0.265550 5_3:+65148823 Msa0825370:CDS
GGTAGTGAGTGCTCTTTATT+TGG 0.277202 5_3:+65149554 Msa0825370:three_prime_UTR
GAAATACAAGAATTCTTCTA+AGG 0.281648 5_3:-65147939 None:intergenic
CCATTGAACTAGACGATTCA+TGG 0.288711 5_3:-65147993 None:intergenic
CAATTGCATCTTTCATAGTT+AGG 0.300214 5_3:+65148246 Msa0825370:CDS
GCTTGCTCTATTTGGCCTAA+TGG 0.324580 5_3:+65148831 Msa0825370:CDS
GATAGCTTTGTGTGGGAGAT+TGG 0.337706 5_3:+65148469 Msa0825370:CDS
AGAATTTCTTATACATAGAT+TGG 0.343658 5_3:+65149124 Msa0825370:CDS
ACTTGTCAGCTGCATGTAAT+AGG 0.350791 5_3:-65149148 None:intergenic
TCAAAGAAATTCTTGATGCT+TGG 0.368629 5_3:+65149393 Msa0825370:CDS
AGATAAACTTCTTAGCAATA+AGG 0.369571 5_3:+65148447 Msa0825370:CDS
AGCAAAATCTTGACTCAATC+AGG 0.374788 5_3:+65148290 Msa0825370:CDS
GTTAGGATTCTTAGTTATGC+TGG 0.386999 5_3:+65148263 Msa0825370:CDS
AGGGTTGTGTATGATTGTTT+TGG 0.399192 5_3:-65148222 None:intergenic
GCCATAAAGAAAATTTATAA+AGG 0.401897 5_3:-65149087 None:intergenic
TCAAAATAGCCATGTGGAAC+TGG 0.403978 5_3:+65148062 Msa0825370:CDS
TTCTCTTATCATTCGACCTA+GGG 0.404879 5_3:-65148892 None:intergenic
CACAATCCAAGTTGTCAACT+TGG 0.433648 5_3:-65147862 None:intergenic
CTAACTATGAAAGATGCAAT+TGG 0.440177 5_3:-65148245 None:intergenic
ATGTATATAACTATATATAT+AGG 0.448250 5_3:+65149533 Msa0825370:three_prime_UTR
CCATGAATCGTCTAGTTCAA+TGG 0.453483 5_3:+65147993 Msa0825370:CDS
TCATTCGACCTAGGGATTCA+AGG 0.458286 5_3:-65148884 None:intergenic
CTAAACATGGATAGGTTGTT+CGG 0.462308 5_3:+65149468 Msa0825370:three_prime_UTR
CTCTGCTGCATGAATCCATT+AGG 0.476375 5_3:-65148846 None:intergenic
TGTCAACTTGGAAACATGAT+AGG 0.478674 5_3:-65147850 None:intergenic
CTTCTCTTATCATTCGACCT+AGG 0.496175 5_3:-65148893 None:intergenic
ATGTTTCCAAGTTGACAACT+TGG 0.509102 5_3:+65147856 Msa0825370:five_prime_UTR
GAAATAACATTGTCTAGGAC+AGG 0.509771 5_3:-65147781 None:intergenic
GGCAAAATATATGATATTCA+GGG 0.516583 5_3:+65149317 Msa0825370:CDS
CATTCGACCTAGGGATTCAA+GGG 0.532711 5_3:-65148883 None:intergenic
AGAAGGAAATAACATTGTCT+AGG 0.540582 5_3:-65147786 None:intergenic
GAGATTGGAGTTCCATTACC+TGG 0.545273 5_3:+65148484 Msa0825370:CDS
ATGAGCTCTTATGCTTCCCT+CGG 0.548636 5_3:-65148949 None:intergenic
AGAAAGAAAGAAACAAAAGA+AGG 0.552175 5_3:-65147803 None:intergenic
AGATTCTACTAAACATGGAT+AGG 0.562341 5_3:+65149460 Msa0825370:three_prime_UTR
CAATAAGGATAGCTTTGTGT+GGG 0.566302 5_3:+65148462 Msa0825370:CDS
ACTATGAAAGATGCAATTGG+AGG 0.571379 5_3:-65148242 None:intergenic
TTTGAAAATTACATGTCCAA+AGG 0.574384 5_3:+65148537 Msa0825370:CDS
GCAGCGGCCCTTGAATCCCT+AGG 0.588324 5_3:+65148876 Msa0825370:CDS
AACAATCAACGTCAATTCCG+AGG 0.588705 5_3:+65148932 Msa0825370:CDS
AGTTGACAACTTGGATTGTG+AGG 0.593060 5_3:+65147865 Msa0825370:five_prime_UTR
CTATGAAAGATGCAATTGGA+GGG 0.614089 5_3:-65148241 None:intergenic
AGACTAGATTCTACTAAACA+TGG 0.614589 5_3:+65149455 Msa0825370:three_prime_UTR
GATATGAAGGCATTTATCAG+TGG 0.615660 5_3:+65148157 Msa0825370:intron
GCAATAAGGATAGCTTTGTG+TGG 0.617349 5_3:+65148461 Msa0825370:CDS
ACAATCAACGTCAATTCCGA+GGG 0.626764 5_3:+65148933 Msa0825370:CDS
TTGACTTTACCAGTTCCACA+TGG 0.646739 5_3:-65148071 None:intergenic
ATAACATTGTCTAGGACAGG+AGG 0.649366 5_3:-65147778 None:intergenic
GAAAACATGCTGCGAAACAC+AGG 0.659506 5_3:-65148996 None:intergenic
TTATCAGTGGAAAAGAACTG+TGG 0.666641 5_3:+65148170 Msa0825370:CDS
CATTAGAGTGCTAAACACAA+AGG 0.666694 5_3:-65149584 None:intergenic
AAAAGATTGAATTGACATGT+CGG 0.667795 5_3:-65149060 None:intergenic
GCAGCAGAGACAAAATGCAG+CGG 0.684257 5_3:+65148860 Msa0825370:CDS
ATCCATTCAAAATAGCCATG+TGG 0.702367 5_3:+65148056 Msa0825370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAACAAAATATAAAGTAAA+TGG - chr5_3:65148692-65148711 None:intergenic 10.0%
!! ATGTATATAACTATATATAT+AGG + chr5_3:65149533-65149552 Msa0825370:three_prime_UTR 10.0%
!! TAAACAAAATATAAAGTAAA+TGG - chr5_3:65148692-65148711 None:intergenic 10.0%
!! ATGTATATAACTATATATAT+AGG + chr5_3:65149533-65149552 Msa0825370:three_prime_UTR 10.0%
!!! ATCAAGGAAAATTTTAAATT+AGG - chr5_3:65148792-65148811 None:intergenic 15.0%
!!! ACCTTTATAAATTTTCTTTA+TGG + chr5_3:65149086-65149105 Msa0825370:CDS 15.0%
!! AGAAAAAAATAAAACAATCA+TGG - chr5_3:65149505-65149524 None:intergenic 15.0%
!!! ATCAAGGAAAATTTTAAATT+AGG - chr5_3:65148792-65148811 None:intergenic 15.0%
!!! ACCTTTATAAATTTTCTTTA+TGG + chr5_3:65149086-65149105 Msa0825370:CDS 15.0%
!! AGAAAAAAATAAAACAATCA+TGG - chr5_3:65149505-65149524 None:intergenic 15.0%
!! ATTATATACTTACATACCTT+TGG - chr5_3:65148556-65148575 None:intergenic 20.0%
!! ATGGATAACTAATAAAAGTA+TGG - chr5_3:65148583-65148602 None:intergenic 20.0%
!! GCCATAAAGAAAATTTATAA+AGG - chr5_3:65149090-65149109 None:intergenic 20.0%
!! AGAATTTCTTATACATAGAT+TGG + chr5_3:65149124-65149143 Msa0825370:CDS 20.0%
!!! TCTTCCAAATTTTTAAAGAT+AGG + chr5_3:65149431-65149450 Msa0825370:three_prime_UTR 20.0%
!!! TCTTCCTATCTTTAAAAATT+TGG - chr5_3:65149438-65149457 None:intergenic 20.0%
!! ATTATATACTTACATACCTT+TGG - chr5_3:65148556-65148575 None:intergenic 20.0%
!! ATGGATAACTAATAAAAGTA+TGG - chr5_3:65148583-65148602 None:intergenic 20.0%
!! GCCATAAAGAAAATTTATAA+AGG - chr5_3:65149090-65149109 None:intergenic 20.0%
!! AGAATTTCTTATACATAGAT+TGG + chr5_3:65149124-65149143 Msa0825370:CDS 20.0%
!!! TCTTCCAAATTTTTAAAGAT+AGG + chr5_3:65149431-65149450 Msa0825370:three_prime_UTR 20.0%
!!! TCTTCCTATCTTTAAAAATT+TGG - chr5_3:65149438-65149457 None:intergenic 20.0%
! AGAAAGAAAGAAACAAAAGA+AGG - chr5_3:65147806-65147825 None:intergenic 25.0%
! GAAATACAAGAATTCTTCTA+AGG - chr5_3:65147942-65147961 None:intergenic 25.0%
!!! GTTTTGTTCTTATGATATGA+AGG + chr5_3:65148144-65148163 Msa0825370:intron 25.0%
! AGATAAACTTCTTAGCAATA+AGG + chr5_3:65148447-65148466 Msa0825370:CDS 25.0%
!! ATTATGAATTTTCCAGGTAA+TGG - chr5_3:65148499-65148518 None:intergenic 25.0%
!! ATGTCAATTATGAATTTTCC+AGG - chr5_3:65148505-65148524 None:intergenic 25.0%
! TTTGAAAATTACATGTCCAA+AGG + chr5_3:65148537-65148556 Msa0825370:CDS 25.0%
!! ACTTTTATTAGTTATCCATG+TGG + chr5_3:65148584-65148603 Msa0825370:intron 25.0%
!!! ATAATCATAGTATTGATCTG+AGG + chr5_3:65148743-65148762 Msa0825370:intron 25.0%
!!! TAATCATAGTATTGATCTGA+GGG + chr5_3:65148744-65148763 Msa0825370:intron 25.0%
!!! ATTTTCCTTGATTTGAATGA+AGG + chr5_3:65148800-65148819 Msa0825370:intron 25.0%
!!! TTTTCCTTGATTTGAATGAA+GGG + chr5_3:65148801-65148820 Msa0825370:intron 25.0%
! AAAAGATTGAATTGACATGT+CGG - chr5_3:65149063-65149082 None:intergenic 25.0%
!!! TGATATTTTTGAGTTAAGAG+AGG + chr5_3:65149172-65149191 Msa0825370:CDS 25.0%
! ATTGATACAAAGTTGCAATT+TGG - chr5_3:65149253-65149272 None:intergenic 25.0%
! ATGCAATATCTTGTGAAATT+TGG + chr5_3:65149296-65149315 Msa0825370:CDS 25.0%
! TGGCAAAATATATGATATTC+AGG + chr5_3:65149316-65149335 Msa0825370:CDS 25.0%
! GGCAAAATATATGATATTCA+GGG + chr5_3:65149317-65149336 Msa0825370:CDS 25.0%
! AGAAAGAAAGAAACAAAAGA+AGG - chr5_3:65147806-65147825 None:intergenic 25.0%
! GAAATACAAGAATTCTTCTA+AGG - chr5_3:65147942-65147961 None:intergenic 25.0%
!!! GTTTTGTTCTTATGATATGA+AGG + chr5_3:65148144-65148163 Msa0825370:intron 25.0%
! AGATAAACTTCTTAGCAATA+AGG + chr5_3:65148447-65148466 Msa0825370:CDS 25.0%
!! ATTATGAATTTTCCAGGTAA+TGG - chr5_3:65148499-65148518 None:intergenic 25.0%
!! ATGTCAATTATGAATTTTCC+AGG - chr5_3:65148505-65148524 None:intergenic 25.0%
! TTTGAAAATTACATGTCCAA+AGG + chr5_3:65148537-65148556 Msa0825370:CDS 25.0%
!! ACTTTTATTAGTTATCCATG+TGG + chr5_3:65148584-65148603 Msa0825370:intron 25.0%
!!! ATAATCATAGTATTGATCTG+AGG + chr5_3:65148743-65148762 Msa0825370:intron 25.0%
!!! TAATCATAGTATTGATCTGA+GGG + chr5_3:65148744-65148763 Msa0825370:intron 25.0%
!!! ATTTTCCTTGATTTGAATGA+AGG + chr5_3:65148800-65148819 Msa0825370:intron 25.0%
!!! TTTTCCTTGATTTGAATGAA+GGG + chr5_3:65148801-65148820 Msa0825370:intron 25.0%
! AAAAGATTGAATTGACATGT+CGG - chr5_3:65149063-65149082 None:intergenic 25.0%
!!! TGATATTTTTGAGTTAAGAG+AGG + chr5_3:65149172-65149191 Msa0825370:CDS 25.0%
! ATTGATACAAAGTTGCAATT+TGG - chr5_3:65149253-65149272 None:intergenic 25.0%
! ATGCAATATCTTGTGAAATT+TGG + chr5_3:65149296-65149315 Msa0825370:CDS 25.0%
! TGGCAAAATATATGATATTC+AGG + chr5_3:65149316-65149335 Msa0825370:CDS 25.0%
! GGCAAAATATATGATATTCA+GGG + chr5_3:65149317-65149336 Msa0825370:CDS 25.0%
AGAAGGAAATAACATTGTCT+AGG - chr5_3:65147789-65147808 None:intergenic 30.0%
!!! ATGATTGTTTTGGACATTTC+TGG - chr5_3:65148215-65148234 None:intergenic 30.0%
CTAACTATGAAAGATGCAAT+TGG - chr5_3:65148248-65148267 None:intergenic 30.0%
CAATTGCATCTTTCATAGTT+AGG + chr5_3:65148246-65148265 Msa0825370:CDS 30.0%
!!! CTTTTATTTCTACATCAGTG+TGG - chr5_3:65148429-65148448 None:intergenic 30.0%
CATAATTGACATTGAGTTTC+TGG + chr5_3:65148513-65148532 Msa0825370:CDS 30.0%
! CATGTTTTCACATAATGTCA+AGG + chr5_3:65149010-65149029 Msa0825370:CDS 30.0%
TCAAAGAAATTCTTGATGCT+TGG + chr5_3:65149393-65149412 Msa0825370:CDS 30.0%
AGACTAGATTCTACTAAACA+TGG + chr5_3:65149455-65149474 Msa0825370:three_prime_UTR 30.0%
AGATTCTACTAAACATGGAT+AGG + chr5_3:65149460-65149479 Msa0825370:three_prime_UTR 30.0%
AGAAGGAAATAACATTGTCT+AGG - chr5_3:65147789-65147808 None:intergenic 30.0%
!!! ATGATTGTTTTGGACATTTC+TGG - chr5_3:65148215-65148234 None:intergenic 30.0%
CTAACTATGAAAGATGCAAT+TGG - chr5_3:65148248-65148267 None:intergenic 30.0%
CAATTGCATCTTTCATAGTT+AGG + chr5_3:65148246-65148265 Msa0825370:CDS 30.0%
!!! CTTTTATTTCTACATCAGTG+TGG - chr5_3:65148429-65148448 None:intergenic 30.0%
CATAATTGACATTGAGTTTC+TGG + chr5_3:65148513-65148532 Msa0825370:CDS 30.0%
! CATGTTTTCACATAATGTCA+AGG + chr5_3:65149010-65149029 Msa0825370:CDS 30.0%
TCAAAGAAATTCTTGATGCT+TGG + chr5_3:65149393-65149412 Msa0825370:CDS 30.0%
AGACTAGATTCTACTAAACA+TGG + chr5_3:65149455-65149474 Msa0825370:three_prime_UTR 30.0%
AGATTCTACTAAACATGGAT+AGG + chr5_3:65149460-65149479 Msa0825370:three_prime_UTR 30.0%
! GAAATAACATTGTCTAGGAC+AGG - chr5_3:65147784-65147803 None:intergenic 35.0%
TGTCAACTTGGAAACATGAT+AGG - chr5_3:65147853-65147872 None:intergenic 35.0%
! ATGTTTCCAAGTTGACAACT+TGG + chr5_3:65147856-65147875 Msa0825370:five_prime_UTR 35.0%
ATCCATTCAAAATAGCCATG+TGG + chr5_3:65148056-65148075 Msa0825370:CDS 35.0%
!! TTCCACATGGCTATTTTGAA+TGG - chr5_3:65148061-65148080 None:intergenic 35.0%
!! GATATGAAGGCATTTATCAG+TGG + chr5_3:65148157-65148176 Msa0825370:intron 35.0%
TTATCAGTGGAAAAGAACTG+TGG + chr5_3:65148170-65148189 Msa0825370:CDS 35.0%
! AGGGTTGTGTATGATTGTTT+TGG - chr5_3:65148225-65148244 None:intergenic 35.0%
CTATGAAAGATGCAATTGGA+GGG - chr5_3:65148244-65148263 None:intergenic 35.0%
ACTATGAAAGATGCAATTGG+AGG - chr5_3:65148245-65148264 None:intergenic 35.0%
GTTAGGATTCTTAGTTATGC+TGG + chr5_3:65148263-65148282 Msa0825370:CDS 35.0%
AGCAAAATCTTGACTCAATC+AGG + chr5_3:65148290-65148309 Msa0825370:CDS 35.0%
!! CAATAAGGATAGCTTTGTGT+GGG + chr5_3:65148462-65148481 Msa0825370:CDS 35.0%
TTCTCTTATCATTCGACCTA+GGG - chr5_3:65148895-65148914 None:intergenic 35.0%
CTAAACATGGATAGGTTGTT+CGG + chr5_3:65149468-65149487 Msa0825370:three_prime_UTR 35.0%
! CATTAGAGTGCTAAACACAA+AGG - chr5_3:65149587-65149606 None:intergenic 35.0%
! GAAATAACATTGTCTAGGAC+AGG - chr5_3:65147784-65147803 None:intergenic 35.0%
TGTCAACTTGGAAACATGAT+AGG - chr5_3:65147853-65147872 None:intergenic 35.0%
! ATGTTTCCAAGTTGACAACT+TGG + chr5_3:65147856-65147875 Msa0825370:five_prime_UTR 35.0%
ATCCATTCAAAATAGCCATG+TGG + chr5_3:65148056-65148075 Msa0825370:CDS 35.0%
!! TTCCACATGGCTATTTTGAA+TGG - chr5_3:65148061-65148080 None:intergenic 35.0%
!! GATATGAAGGCATTTATCAG+TGG + chr5_3:65148157-65148176 Msa0825370:intron 35.0%
TTATCAGTGGAAAAGAACTG+TGG + chr5_3:65148170-65148189 Msa0825370:CDS 35.0%
! AGGGTTGTGTATGATTGTTT+TGG - chr5_3:65148225-65148244 None:intergenic 35.0%
CTATGAAAGATGCAATTGGA+GGG - chr5_3:65148244-65148263 None:intergenic 35.0%
ACTATGAAAGATGCAATTGG+AGG - chr5_3:65148245-65148264 None:intergenic 35.0%
GTTAGGATTCTTAGTTATGC+TGG + chr5_3:65148263-65148282 Msa0825370:CDS 35.0%
AGCAAAATCTTGACTCAATC+AGG + chr5_3:65148290-65148309 Msa0825370:CDS 35.0%
!! CAATAAGGATAGCTTTGTGT+GGG + chr5_3:65148462-65148481 Msa0825370:CDS 35.0%
TTCTCTTATCATTCGACCTA+GGG - chr5_3:65148895-65148914 None:intergenic 35.0%
CTAAACATGGATAGGTTGTT+CGG + chr5_3:65149468-65149487 Msa0825370:three_prime_UTR 35.0%
! CATTAGAGTGCTAAACACAA+AGG - chr5_3:65149587-65149606 None:intergenic 35.0%
! ATAACATTGTCTAGGACAGG+AGG - chr5_3:65147781-65147800 None:intergenic 40.0%
CACAATCCAAGTTGTCAACT+TGG - chr5_3:65147865-65147884 None:intergenic 40.0%
AGTTGACAACTTGGATTGTG+AGG + chr5_3:65147865-65147884 Msa0825370:five_prime_UTR 40.0%
CCATTGAACTAGACGATTCA+TGG - chr5_3:65147996-65148015 None:intergenic 40.0%
! CCATGAATCGTCTAGTTCAA+TGG + chr5_3:65147993-65148012 Msa0825370:CDS 40.0%
TCAAAATAGCCATGTGGAAC+TGG + chr5_3:65148062-65148081 Msa0825370:CDS 40.0%
TTGACTTTACCAGTTCCACA+TGG - chr5_3:65148074-65148093 None:intergenic 40.0%
!! GCAATAAGGATAGCTTTGTG+TGG + chr5_3:65148461-65148480 Msa0825370:CDS 40.0%
ATTTCTGAGTCAGTACCACA+TGG - chr5_3:65148602-65148621 None:intergenic 40.0%
! TCCTTGATTTGAATGAAGGG+TGG + chr5_3:65148804-65148823 Msa0825370:intron 40.0%
! CCTTGATTTGAATGAAGGGT+GGG + chr5_3:65148805-65148824 Msa0825370:intron 40.0%
! CTTGATTTGAATGAAGGGTG+GGG + chr5_3:65148806-65148825 Msa0825370:intron 40.0%
CTTCTCTTATCATTCGACCT+AGG - chr5_3:65148896-65148915 None:intergenic 40.0%
AACAATCAACGTCAATTCCG+AGG + chr5_3:65148932-65148951 Msa0825370:CDS 40.0%
ACAATCAACGTCAATTCCGA+GGG + chr5_3:65148933-65148952 Msa0825370:CDS 40.0%
ACTTGTCAGCTGCATGTAAT+AGG - chr5_3:65149151-65149170 None:intergenic 40.0%
TGGATAGGTTGTTCGGTTAT+TGG + chr5_3:65149475-65149494 Msa0825370:three_prime_UTR 40.0%
GGTAGTGAGTGCTCTTTATT+TGG + chr5_3:65149554-65149573 Msa0825370:three_prime_UTR 40.0%
! ATAACATTGTCTAGGACAGG+AGG - chr5_3:65147781-65147800 None:intergenic 40.0%
CACAATCCAAGTTGTCAACT+TGG - chr5_3:65147865-65147884 None:intergenic 40.0%
AGTTGACAACTTGGATTGTG+AGG + chr5_3:65147865-65147884 Msa0825370:five_prime_UTR 40.0%
CCATTGAACTAGACGATTCA+TGG - chr5_3:65147996-65148015 None:intergenic 40.0%
! CCATGAATCGTCTAGTTCAA+TGG + chr5_3:65147993-65148012 Msa0825370:CDS 40.0%
TCAAAATAGCCATGTGGAAC+TGG + chr5_3:65148062-65148081 Msa0825370:CDS 40.0%
TTGACTTTACCAGTTCCACA+TGG - chr5_3:65148074-65148093 None:intergenic 40.0%
!! GCAATAAGGATAGCTTTGTG+TGG + chr5_3:65148461-65148480 Msa0825370:CDS 40.0%
ATTTCTGAGTCAGTACCACA+TGG - chr5_3:65148602-65148621 None:intergenic 40.0%
! TCCTTGATTTGAATGAAGGG+TGG + chr5_3:65148804-65148823 Msa0825370:intron 40.0%
! CCTTGATTTGAATGAAGGGT+GGG + chr5_3:65148805-65148824 Msa0825370:intron 40.0%
! CTTGATTTGAATGAAGGGTG+GGG + chr5_3:65148806-65148825 Msa0825370:intron 40.0%
CTTCTCTTATCATTCGACCT+AGG - chr5_3:65148896-65148915 None:intergenic 40.0%
AACAATCAACGTCAATTCCG+AGG + chr5_3:65148932-65148951 Msa0825370:CDS 40.0%
ACAATCAACGTCAATTCCGA+GGG + chr5_3:65148933-65148952 Msa0825370:CDS 40.0%
ACTTGTCAGCTGCATGTAAT+AGG - chr5_3:65149151-65149170 None:intergenic 40.0%
TGGATAGGTTGTTCGGTTAT+TGG + chr5_3:65149475-65149494 Msa0825370:three_prime_UTR 40.0%
GGTAGTGAGTGCTCTTTATT+TGG + chr5_3:65149554-65149573 Msa0825370:three_prime_UTR 40.0%
!! GATAGCTTTGTGTGGGAGAT+TGG + chr5_3:65148469-65148488 Msa0825370:CDS 45.0%
GAGATTGGAGTTCCATTACC+TGG + chr5_3:65148484-65148503 Msa0825370:CDS 45.0%
CCCACCCTTCATTCAAATCA+AGG - chr5_3:65148808-65148827 None:intergenic 45.0%
GCTTGCTCTATTTGGCCTAA+TGG + chr5_3:65148831-65148850 Msa0825370:CDS 45.0%
CTCTGCTGCATGAATCCATT+AGG - chr5_3:65148849-65148868 None:intergenic 45.0%
CATTCGACCTAGGGATTCAA+GGG - chr5_3:65148886-65148905 None:intergenic 45.0%
TCATTCGACCTAGGGATTCA+AGG - chr5_3:65148887-65148906 None:intergenic 45.0%
ATGAGCTCTTATGCTTCCCT+CGG - chr5_3:65148952-65148971 None:intergenic 45.0%
GAAAACATGCTGCGAAACAC+AGG - chr5_3:65148999-65149018 None:intergenic 45.0%
!! GATAGCTTTGTGTGGGAGAT+TGG + chr5_3:65148469-65148488 Msa0825370:CDS 45.0%
GAGATTGGAGTTCCATTACC+TGG + chr5_3:65148484-65148503 Msa0825370:CDS 45.0%
CCCACCCTTCATTCAAATCA+AGG - chr5_3:65148808-65148827 None:intergenic 45.0%
GCTTGCTCTATTTGGCCTAA+TGG + chr5_3:65148831-65148850 Msa0825370:CDS 45.0%
CTCTGCTGCATGAATCCATT+AGG - chr5_3:65148849-65148868 None:intergenic 45.0%
CATTCGACCTAGGGATTCAA+GGG - chr5_3:65148886-65148905 None:intergenic 45.0%
TCATTCGACCTAGGGATTCA+AGG - chr5_3:65148887-65148906 None:intergenic 45.0%
ATGAGCTCTTATGCTTCCCT+CGG - chr5_3:65148952-65148971 None:intergenic 45.0%
GAAAACATGCTGCGAAACAC+AGG - chr5_3:65148999-65149018 None:intergenic 45.0%
! GTGGGGAAGCTTGCTCTATT+TGG + chr5_3:65148823-65148842 Msa0825370:CDS 50.0%
GCAGCAGAGACAAAATGCAG+CGG + chr5_3:65148860-65148879 Msa0825370:CDS 50.0%
! GTGGGGAAGCTTGCTCTATT+TGG + chr5_3:65148823-65148842 Msa0825370:CDS 50.0%
GCAGCAGAGACAAAATGCAG+CGG + chr5_3:65148860-65148879 Msa0825370:CDS 50.0%
GCAGCGGCCCTTGAATCCCT+AGG + chr5_3:65148876-65148895 Msa0825370:CDS 65.0%
GCAGCGGCCCTTGAATCCCT+AGG + chr5_3:65148876-65148895 Msa0825370:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 65147763 65149631 65147763 ID=Msa0825370;Name=Msa0825370
chr5_3 mRNA 65147763 65149631 65147763 ID=Msa0825370-mRNA-1;Parent=Msa0825370;Name=Msa0825370-mRNA-1;_AED=0.10;_eAED=0.10;_QI=202|1|1|1|1|1|4|207|328
chr5_3 exon 65147763 65148083 65147763 ID=Msa0825370-mRNA-1:exon:20367;Parent=Msa0825370-mRNA-1
chr5_3 exon 65148166 65148311 65148166 ID=Msa0825370-mRNA-1:exon:20368;Parent=Msa0825370-mRNA-1
chr5_3 exon 65148440 65148558 65148440 ID=Msa0825370-mRNA-1:exon:20369;Parent=Msa0825370-mRNA-1
chr5_3 exon 65148822 65149631 65148822 ID=Msa0825370-mRNA-1:exon:20370;Parent=Msa0825370-mRNA-1
chr5_3 five_prime_UTR 65147763 65147964 65147763 ID=Msa0825370-mRNA-1:five_prime_utr;Parent=Msa0825370-mRNA-1
chr5_3 CDS 65147965 65148083 65147965 ID=Msa0825370-mRNA-1:cds;Parent=Msa0825370-mRNA-1
chr5_3 CDS 65148166 65148311 65148166 ID=Msa0825370-mRNA-1:cds;Parent=Msa0825370-mRNA-1
chr5_3 CDS 65148440 65148558 65148440 ID=Msa0825370-mRNA-1:cds;Parent=Msa0825370-mRNA-1
chr5_3 CDS 65148822 65149424 65148822 ID=Msa0825370-mRNA-1:cds;Parent=Msa0825370-mRNA-1
chr5_3 three_prime_UTR 65149425 65149631 65149425 ID=Msa0825370-mRNA-1:three_prime_utr;Parent=Msa0825370-mRNA-1
Gene Sequence

>Msa0825370

ATGAATGATTATGCATACAAAGTTGAAACCATGAATCGTCTAGTTCAATGGAAAATTCACAATTTATCAACATGCACTTATATCAAAGCTGATCCATTCAAAATAGCCATGTGGAACTGGCATTTATCAGTGGAAAAGAACTGTGGTTTACGTATTGAATTATATCCAGAAATGTCCAAAACAATCATACACAACCCTCCAATTGCATCTTTCATAGTTAGGATTCTTAGTTATGCTGGAAATAGCAAAATCTTGACTCAATCAGAAATAAAAGATAAACTTCTTAGCAATAAGGATAGCTTTGTGTGGGAGATTGGAGTTCCATTACCTGGAAAATTCATAATTGACATTGAGTTTCTGGATTTGAAAATTACATGTCCAAAGGGTGGGGAAGCTTGCTCTATTTGGCCTAATGGATTCATGCAGCAGAGACAAAATGCAGCGGCCCTTGAATCCCTAGGTCGAATGATAAGAGAAGATATCTATACAGACATAACAATCAACGTCAATTCCGAGGGAAGCATAAGAGCTCATCGAGCTGTTCTTGCTTCACAATCACCTGTGTTTCGCAGCATGTTTTCACATAATGTCAAGGAGACTGATTTGTCTACGATAAACATCACCGACATGTCAATTCAATCTTTTCAGACCTTTATAAATTTTCTTTATGGCAATGTCAATGATGAAGAATTTCTTATACATAGATTGGACCTATTACATGCAGCTGACAAGTATGATATTTTTGAGTTAAGAGAGGCATGTGAAAAGAGTCTTCAAGAAGATATTGATCTAAAAAATGTACTTGAGAGACTCCAAATTGCAACTTTGTATCAATTAACAACGTTGAAGTTTAGTTGTATGCAATATCTTGTGAAATTTGGCAAAATATATGATATTCAGGGTGATTTAAGTTCTTTCTTGCAAACTGCTGATAGAGATTTGATATGTGAAGTCTTCAAAGAAATTCTTGATGCTTGGAAAAAGTGA
Protein sequence

>Msa0825370

MNDYAYKVETMNRLVQWKIHNLSTCTYIKADPFKIAMWNWHLSVEKNCGLRIELYPEMSKTIIHNPPIASFIVRILSYAGNSKILTQSEIKDKLLSNKDSFVWEIGVPLPGKFIIDIEFLDLKITCPKGGEACSIWPNGFMQQRQNAAALESLGRMIREDIYTDITINVNSEGSIRAHRAVLASQSPVFRSMFSHNVKETDLSTINITDMSIQSFQTFINFLYGNVNDEEFLIHRLDLLHAADKYDIFELREACEKSLQEDIDLKNVLERLQIATLYQLTTLKFSCMQYLVKFGKIYDIQGDLSSFLQTADRDLICEVFKEILDAWKK*